Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of cd4+ t cell responses in mice infected with schistosoma japonicum. | to better understand the interaction between schistosoma japonicum and its murine host, we characterized the immune response of cd4+ t cells generated during an experimental s. japonicum infection based on different key aspects, from gene expression to cell behavior. mouse oligonucleotide microarrays were used to compare gene expression profiles of cd4+ t cells from spleens of mice at 0, 3, 6 and 13 weeks post-infection. flow cytometry analysis was used to determine type 1 and type 2 cytokine-se ... | 2006 | 16680373 |
| expression and characterization of lactate dehydrogenase from schistosoma japonicum. | some effective antiparasitic drugs for chemotherapy schistosomiasis, such as praziquantel and artemether, are proved to act on lactate dehydrogenase (ldh) in vitro, but detailed molecular action mechanisms of these have not yet been elucidated. to this end, the sequence encoding ldh of schistosoma japonicum (sjldh) was amplified from the cdna library of the adult parasite. sequence analysis revealed an open reading frame (orf) of 999 bp, encoding 332 amino acids with a molecular weight of 36.120 ... | 2006 | 16688469 |
| translation of 'rare' codons in a cell-free protein synthesis system from escherichia coli. | we analyzed the effect of nine 'rare' codons (aga, agg, aua, ccc, cga, cgg, cua, gga, and uua) on gene expression in an escherichia coli coupled transcription/translation cell-free system, in comparison with a cell-based expression system. each reporter gene contained five consecutive repeats of a rare codon, or in some experiments, three consecutive repeats. the cell-free expression of the genes bearing the codons cga, cua, gga, and uua was not affected, although these codons, except for gga, w ... | 2006 | 16703415 |
| pubertal development predicts resistance to infection and reinfection with schistosoma japonicum. | in communities where schistosoma species are endemic, the prevalence and intensity of schistosomiasis is disproportionately high among children, compared with adults. this epidemiologic pattern is consistent with either the slow development of resistance or the requirement of host developmental changes for the expression of resistance. | 2006 | 16705573 |
| new insights into the observed age-specific resistance to reinfection with schistosoma japonicum. | 2006 | 16705574 | |
| bayesian estimation of community prevalences of schistosoma japonicum infection in china. | a bayesian approach to overcome the imperfections of an immunological test (an antibody-based elisa) and a parasitological test (kato-katz) in the detection of schistosoma japonicum infection, was used to estimate community prevalences of s. japonicum infection in china. at the same time, the similarity between the prevalence estimates based on data from elisa alone and those using data from both elisa and kato-katz tests was explored. the database from the third nationwide sampling survey of sc ... | 2006 | 16759658 |
| reconstruction and in silico analysis of the mapk signaling pathways in the human blood fluke, schistosoma japonicum. | at present, little is known about signal transduction mechanisms in schistosomes, which cause the disease of schistosomiasis. the mitogen-activated protein kinase (mapk) signaling pathways, which are evolutionarily conserved from yeast to homo sapiens, play key roles in multiple cellular processes. here, we reconstructed the hypothetical mapk signaling pathways in schistosoma japonicum and compared the schistosome pathways with those of model eukaryote species. we identified 60 homologous compon ... | 2006 | 16765950 |
| an attempt to study the economic loss arising from schistosoma japonicum infection and the benefits derived from treatment. | from the northern and southern portions of leyte province, which are endemic for schistosomiasis, a total of 801 infected individuals were interviewed, examined, and classified into mild, moderate, severe and very severe forms of disease with an assumed loss of working capacity for each category. the frequency rate or number of spells of illness for the past year under observation were correlated with the degree of incapacity to get the total days lost per person per year. following a series of ... | 2006 | 16771209 |
| concurrent comparison of three water contact measurement tools in four endemic villages of the philippines. the schistosomiasis transmission ecology in the philippines project (step). | schistosomiasis japonica is a chronic helminthic infection contracted through contact with water infested with schistosoma japonicum. the infection is associated with severe disease and is an important public health concern in philippines. | 2006 | 16772005 |
| cross-sectional associations between intensity of animal and human infection with schistosoma japonicum in western samar province, philippines. | to estimate the association between the intensity of animal infection with schistosoma japonicum and human infection in western samar province, the philippines. | 2006 | 16799728 |
| a contributory role for activated hepatic stellate cells in the dynamics of schistosoma japonicum egg-induced fibrosis. | the disease manifestations of schistosomiasis arise from the mammalian host-mediated type 2 t-helper cell-induced (th2) fibro-granulomatous inflammatory response to eggs trapped within host tissues. activated hepatic stellate cells are well described as the effector cells of hepatic fibrosis in a variety of human diseases and rodent models. the aim of this study was to further understand the mechanism of fibrosis and the role of hepatic stellate cells in hepatic schistosomiasis progression. grou ... | 2006 | 16806222 |
| 2-cys peroxiredoxins from schistosoma japonicum: the expression profile and localization in the life cycle. | peroxiredoxin (prx) is known to be an antioxidant protein that protects the organisms against various oxidative stresses and functions as a signal transductor. here, we determined the full-length cdna sequences of three types of prx from an asian blood fluke, schistosoma japonicum: prx-1, prx-2 and prx-3. according to the deduced amino acid sequences, only prx-3 had a mitochondria-targeting sequence. using rt-pcr, it was shown that these prx genes were constitutively expressed in the eggs, cerca ... | 2006 | 16806527 |
| the history of schistosomiasis japonica in taiwan. | studies of schistosomiasis japonica in taiwan indicate that the formosan strain of schistosoma japonicum is zoophilic in nature. after the discovery of the adult worm of s. japonicum in 1914, a considerable number of epidemiologic studies and experimental infection studies were conducted. this paper is a review of the history of schistosomiasis japonica in taiwan from before world war ii to 2003. | 2006 | 16849099 |
| [anti-schistosomiasis effect induced by full length dna vaccine coding paramyosin of schistosoma japonicum in c57bl/6 mice]. | to observe the anti-schistosomiasis effect in mice immunized with sjc97 dna vaccine. | 2006 | 16862901 |
| [identification of the sjarg novel gene and study on its protective potential as a vaccine]. | to recognize and identify the arginase (arg) gene of schistosoma japonicum(sj), and to study its protection potential as a vaccine. | 2006 | 16862902 |
| [construction and expression of schistosoma japonicum bivalent dna vaccines and their efficiencies of protective immunity]. | to study the efficiency of protective immunity afforded by the constructed bivalent dna vaccines of schistosoma japonicum. | 2006 | 16862904 |
| [immunoscreening of schistosomulum cdna library of schistosoma japonicum and preliminary identification of membrane-associated protein abundant in tegument]. | to obtain genes encoding the novel molecules for diagnosis of schistosomiasis. | 2006 | 16862906 |
| [study on the effect of heluoshugan capsule on liver fibrosis induced by schistosoma japonicum infection in mice]. | to investigate the effect of chinese traditional medicine heluoshugan capsule on liver fibrosis induced by schistosoma japonicum infection in mice. | 2006 | 16862912 |
| [antibody detection in sera of patients with schistosomiasis japonica by dot immunogold filtration assay]. | serum antibody of schistosomiasis patients was detected by dot immunogold filtration method (digfa) in laboratory and field, and f-elisa was used as control. the results showed that there was no significant difference between these two assays in sensitivity and specificity (p > 0.05), with a high coincidence. digfa is easy to operate and may deserve a wide application in the diagnosis of schistosomiasis. | 2006 | 16862917 |
| [inhibition of quercetin on liver fibrosis due to schistosoma japonicum infection and on the expression of immediate early gene and metalloproteinase 1 inhibitor in liver tissue of mice]. | quercetin and praziquantel were used to treat mice with hepatic fibrosis due to schistosoma japonicum infection. quercetin treatment obviously relieved the degree of hepatic fibrosis, significantly reduced the expression of immediate early gene, tissue inhibitor of metalloproteinase 1 (timp 1), types i and iii collagen compared to the control. the expression of c-jun mrna, type i and type iii collagen were reduced significantly compared to the group treated with praziquantel, whereas no differen ... | 2006 | 16862918 |
| [induction of protective immunity against schistosoma japonicum by pcdna3.1/sj20.8 nucleic acid vaccine in balb/c mice]. | sj20.8 gene was amplified by pcr and inserted into eukaryotic expression plasmid pcdna3.1 to construct recombinant plasmid pcdna3.1/sj20.8, which was then injected into the quadriceps femoris of the balb/c mice. results showed that the sj20.8 antigen was low expressed in the local tissue of the mice, and was not able to significantly reduce eggs in the liver than in the control mice. | 2006 | 16862920 |
| [national surveillance of schistosomiasis in china from 2000 to 2004]. | to specify the trends of endemic situation among twenty one national surveillance sites for schistosomiasis from 2000 to 2004. | 2006 | 16866131 |
| comparison of the role of dendritic cells and macrophages in inducing protective immunity against schistosoma japonicum. | to compare a potential role of dendritic cells (dcs) and macrophages in inducing protective immunity against infection with schistosoma japonicum. | 2006 | 16866135 |
| [construction of the female subtractive cdna library and screening of the specific expressing genes]. | to screen the schistosoma japonicum female specific expressing genes. | 2006 | 16866144 |
| [protective efficacy of co-immunization with sj26 dna and recombinant protein vaccine against schistosoma japonicum in mice]. | to study the protective efficacy of co-immunization with schistosoma japonicum glutathione s-transferase (sj26) dna and recombinant sj26 protein (rsj26 gst) vaccine against schistosoma japonicum in balb/c mice. | 2006 | 16866145 |
| [pigment deposit in liver of balb/c mice infected by schistosoma japonicum and its relation to the effect of praziquantel treatment]. | pigment is a kind of metabolite excreted by schistosome after erythrocyte ingestion. it deposited first in liver sinusoid, then outside and inside of granuloma, and around eggs. the level of pigment was in parallel with the degree of liver fibrosis (p < 0.01). after chemotherapy with praziquantel, the level of pigment decreased considerably (p < 0.01) and the size of granuloma shrinks obviously too (p < 0.01). therefore, the degree of liver fibrosis in mice with schistosome infection and the eff ... | 2006 | 16866157 |
| schistosoma japonicum: the design and experimental evaluation of a multivalent dna vaccine. | the aim of this study was to construct and evaluate the immunity efficacy of the dna multivalent vaccine pvivo(2)sjfabp-23. the vaccine was constructed and produced as follows. forty balb/c mice were divided into four groups designated pvivo(2), pvivo(2)sj23, pvivo(2)sjfabp and pvivo(2)sjfabp-23. each mouse was immunized with 100 mug of the corresponding plasmid dna by intramuscular injection. 28 days post-vaccination, the mice were challenged with s. japonicum cercariae, and the worm and egg bu ... | 2006 | 16874456 |
| does multiple hosts mean multiple parasites? population genetic structure of schistosoma japonicum between definitive host species. | multi-host parasites, those capable of infecting more than one species of host, are responsible for the majority of all zoonotic, emerging or persistent human and animal diseases and are considered one of the major challenges for the biomedical sciences in the 21st century. we characterized the population structure of the multi-host parasite schistosoma japonicum in relation to its definitive host species by genotyping miracidia collected from humans and domestic animals across five villages aro ... | 2006 | 16876170 |
| schistosoma japonicum reinfection after praziquantel treatment causes anemia associated with inflammation. | there is a relationship between schistosomiasis and anemia, although the magnitude and exact mechanisms involved are unclear. in a cohort of 580 schistosoma japonicum-infected 7- to 30-year-old patients from leyte, the philippines, we evaluated the impact of reinfection with s. japonicum after treatment with praziquantel on the mean hemoglobin level, iron-deficiency (ida) and non-iron-deficiency anemia (nida), and inflammatory markers. all participants were treated at baseline and followed up ev ... | 2006 | 16923790 |
| conus medullaris schistosomiasis. | the authors performed a study to investigate the clinical manifestations, treatment strategies, and possible pathogenesis of conus medullaris schistosomiasis. | 2006 | 16925081 |
| [cloning and expression of the succinate dehydrogenase iron-sulfur protein of schistosoma japonicum chinese strain in e. coli]. | to clone the full-length gene encoding succinate dehydrogenase iron-sulfur protein of schistosoma japonicum (sjsdisp) chinese strain and express it in escherichia coli. | 2006 | 16951498 |
| transgene constructs in coho salmon (oncorhynchus kisutch) are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite dna. | currently, little information is available regarding the molecular organization of integrated transgenes in genetically-engineered fish. we performed a detailed structural analysis of an inserted transgene in one strain (m77) of transgenic coho salmon (oncorhynchus kisutch) containing a salmon growth hormone gene construct (onmtgh1). microinjected dna was found to have inserted into a single site in the coho salmon genome, and was organized with four complete internal copies and two partial term ... | 2006 | 16952013 |
| expression of recombinant baculovirus carrying schistosoma japonicum 26 ku gst in mammalian cells. | in order to construct recombinant baculovirus carrying schistosoma japonicum 26 ku glutathione s-transferase gene (sj26), and observe the expression of sj26 in mammalian cells, the sj26 gene was amplified with plasmid pgex-3x as template by pcr, and then recombined into t vector for sequencing. sj26 gene was inserted into the downstream of cmv promoter of donor plasmid pfbdgc, and the recombinant donor plasmid pfbdgc-sj26 transformed into dh10bac, then the recombinant bacmid accmvsj26 was isolat ... | 2006 | 16961264 |
| a growing degree-days based time-series analysis for prediction of schistosoma japonicum transmission in jiangsu province, china. | it has been suggested that global warming may alter the frequency and transmission dynamics of vector-borne diseases. to test this claim for schistosomiasis, we conducted a time-series analysis from 1972-2002 for 39 of the 70 counties of jiangsu province, eastern china, where schistosoma japonicum is partially endemic. we used a modeling approach to estimate the annual growing degree-days (agdd), employing a lower temperature threshold of 15.3 degrees c. our final model included both temporal an ... | 2006 | 16968940 |
| rapid, simple, and sensitive immunoagglutination assay with sio2 particles and quartz crystal microbalance for quantifying schistosoma japonicum antibodies. | the resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. we developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses sio(2) particles and quartz crystal microbalance (qcm) for quantifying schistosoma japonicum (sj) antibodies (sjab). | 2006 | 16990420 |
| protective immunity induced by phage displayed mitochondrial related peptides of schistosoma japonicum. | new antigens and strategies are necessary for vaccine development against schistosomiasis japonica. using a pool of 43 high titred anti-swa sera from individuals residing in an schistosoma japonicum endemic area of china, we have cloned a s. japonicum gene by cdna library screening. the recombinant sj338 protein has 44-46% identity to a mitochondrial precursor receptor protein of humans and rats. immunization of mice with the recombinant sj338 conferred 27-32% (p<0.01) reduction in worm burdens ... | 2006 | 16999929 |
| sigmoid colonic carcinoma associated with deposited ova of schistosoma japonicum: a case report. | we report a case of sigmoid colonic carcinoma associated with deposited ova of schistosoma japonicum. a 57-year old woman presented with a 10-mo history of left lower quadrant abdominal pain and a 2-mo history of bloody stools. she had a significant past medical history of asymptomatic schistosomiasis japonica and constipation. a colonoscopy showed an exophytic fragile neoplasm with an ulcerating surface in the sigmoid colon. during the radical operative procedure, we noted the partially encircl ... | 2006 | 17009414 |
| immunological cross-reactivity analysis on recombinant histamine-releasing factors from schistosoma japonicum, clonorchis sinensis, and wistar rat. | studies have demonstrated a declined incidence of allergic disorders in the population with helminthic infection. though several hypotheses have been proposed to explain how helminthic infection protected people against allergies, the underlying mechanisms remain poorly understood. a human histamine-releasing factor (hrf) has been proved to be closely related to the development of allergic disorders and the homologues are ubiquitously expressed in all eukaryotic organisms including parasites. to ... | 2007 | 17024353 |
| effect of temperature on the development of schistosoma japonicum within oncomelania hupensis, and hibernation of o. hupensis. | the objectives of this investigation were to assess the effect of temperature on the development of schistosoma japonicum harboured in oncomelania hupensis and to determine the lowest temperature threshold at which the hibernation of o. hupensis occurs. in the first experiment, adult infection-free o. hupensis, collected from jiangsu province in eastern china, were infected with s. japonicum miracidia and raised at different temperatures under laboratory conditions. the development of miracidia ... | 2007 | 17031698 |
| pro-inflammatory cytokines and c-reactive protein are associated with undernutrition in the context of schistosoma japonicum infection. | schistosomiasis is associated with undernutrition, but the mechanisms involved remain unknown. we analyzed baseline and follow-up data from a longitudinal treatment-reinfection study in n = 477 schistosoma japonicum-infected subjects 7-20 years of age from leyte, the philippines. after baseline treatment with praziquantel, follow-up visits were scheduled every 3 months for 18 months; stool, venous blood, and anthropometric measurements were collected at each visit. cytokine production by periphe ... | 2006 | 17038701 |
| the role of microscopy in the investigation of paramyosin as a vaccine candidate against schistosoma japonicum. | there has been growing interest in paramyosin as a vaccine component to combat schistosomiasis. immunological and molecular techniques have been used in the past to investigate the effectiveness of a paramyosin vaccine as an anti-schistosomal treatment. however, recent localization studies at ultrastructural and morphological levels have highlighted a number of questions concerning the role of paramyosin within schistosome parasites. debates about how a non-surface protein such as paramyosin mig ... | 1998 | 17040718 |
| the application of attributable risk analysis in helminth epidemiology. | estimating the public health impact of endemic helminth infections is an important component of control strategies that aim not only to reduce the number of infections in a community, but also to improve community health. this is not straightforward, as the risk of morbidity in an infected individual can be influenced by a number of factors, and different infections can cause similar symptoms. here, mark booth describes how attributable risk analysis techniques based on 232 tables can be used to ... | 1998 | 17040864 |
| schistosoma japonicum egg antigens stimulate cd4 cd25 t cells and modulate airway inflammation in a murine model of asthma. | a number of epidemiological and clinical studies have suggested an inverse association between allergy and helminth infection, such as schistosomiasis. therefore, we hypothesize that schistosoma japonicum egg antigens, a type of native antigen, can induce production of cd4(+) cd25(+) t cells with regulatory activity, modulating airway inflammation and inhibiting asthma development. the frequency of cd4(+) cd25(+) t cells was determined by flow cytometry for mice treated with ovalbumin (ova), cd2 ... | 2007 | 17042799 |
| cytokine mrna profiles in pigs exposed prenatally and postnatally to schistosoma japonicum. | the pig is a natural host for schistosoma japonicum and a useful animal model of human infection. the aim of the present study was to assess the differences between the cytokine profiles in prenatally or postnatally s. japonicum exposed pigs. seven prenatally exposed pigs, 7 postnatally exposed pigs and 4 uninfected control pigs were compared 27 weeks post exposure. variables included worm burdens, tissue egg counts, liver pathology and mrna levels of il-2, il-4, il-10, il-12, tnf-alpha, tgf-bet ... | 2007 | 17074293 |
| electrophilic aromatic substituted luciferins as bioluminescent probes for glutathione s-transferase assays. | new highly sensitive latent bioluminescent luciferin substrates were designed and synthesized for monitoring mammalian glutathione s-transferase (gst) and schistosoma japonicum enzyme activities. | 2006 | 17082862 |
| [study on the relationship between the degree of periportal fibrosis, hepatic parenchymatous fibrosis and diameter of portal vein in schistosoma japonicum infection]. | the degree of periportal fibrosis, hepatic parenchymatous fibrosis and the diameter of portal vein in fishermen from highly endemic area of schistosomiasis japonica in dongting lake region were measured. the results showed a significant correlation between the degree of periportal fibrosis and parenchymatous fibrosis and the portal venous diameter with a correlation coefficient of 0.375 and 0.332 respectively. the authors consider that the diameter of the portal vein can be used to assess the he ... | 2006 | 17094594 |
| [protective immunity induced by recombinant schistosoma japonicum thioredoxin in mice]. | to investigate the protective immunity of the recombinant thioredoxin of schistosoma japonicum(resjctrx)in mice. | 2006 | 17094615 |
| [development of a pcr assay for detecting schistosoma japonicum-infected oncomelania hupensis]. | to establish a sensitive and specific pcr assay for detecting schistosoma japonicum-infected oncomelania hupensis. | 2006 | 17094624 |
| immunomodulation of the hepatic egg granuloma in schistosoma japonicum-infected pigs. | immunomodulation of perioval granulomas is a well-known phenomenon in schistosome-infected mice, but only little is known about granuloma modulation in other animal models of human schistosomiasis. in the present study, we explored immunomodulation of egg granulomas in the liver in a pig model of schistosomiasis japonica. granuloma size was measured and t cells, b cells and igg(+) plasma cells in granulomas were quantified in pigs at 9, 12 and 21 weeks post infection (wpi) with schistosoma japon ... | 2006 | 17096648 |
| [study of the efficacy of a monoclonal antibody biotin-avidin system for the diagnosis of schistosomiasis japonica]. | to explore the diagnostic efficiency of circulating antigen using the tm5.28 mab-biotin-avidin system for the detection of schistosomiasis japonica. | 2006 | 17097002 |
| [influence of immunization dose schemes on immunoprotective response to recombinant signaling protein 14-3-3 of schistosoma japonicum]. | to discuss the optimal immunization dose by observing the immunoprotective effects of different doses of recombinant schistosoma japonicum (chinese strain) signaling protein 14-3-3 (rsj14-3-3). | 2006 | 17097003 |
| distribution of mast cells in relation to schistosoma japonicum induced lesions in pigs. | the pathogenesis of schistosomiasis japonica has been extensively studied, however only little attention has been paid to the presence and localization of mast cells in relation to schistosoma japonicum induced lesions. the aim of the present pilot study was to assess the parasitological and pathological responses in s. japonicum infected pigs with emphasis on the description of the distribution of mast cells in relation to lesions in the liver and cecum. six pigs were exposed to 2,000 cercariae ... | 2006 | 17121286 |
| cd4+ t-cell counts, cd4+/cd8+ t-cell count ratios, and antibody levels in migrant fishermen infected with schistosoma japonicum in the dongting lake, china. | in this study, cd4(+) and cd8(+) t cells and antibody levels were measured in 94 migrant fishermen infected with schistosoma japonicum from dongting lake, china. prevalence among these fishermen was high (63.8%), with a mean infection intensity of 61.4 +/- 3.8 epg, and included a high proportion of individuals (39.4%) with substantial parenchymal fibrosis (stages > or = 2/3). the cd4(+)/cd8(+) ratio in men (1.34 +/- 0.11) was significantly lower than that of women (1.58 +/- 0.15). cd4(+) t cells ... | 2006 | 17123986 |
| novel real-time pcr for detection of schistosoma japonicum in stool. | chemotherapy has been used on a large scale in countries where the blood fluke schistosoma japonicum is endemic. this has led to a lower intensity of infections and consequently lower diagnostic values of commonly used diagnostic tests like serology and kato-katz stool smear. we designed a novel real-time pcr method for detection of s. japonicum in stool samples. further, we evaluated different versions of an inexpensive, non-commercial extraction method, rose, as well as the commercial qiaamp d ... | 2006 | 17124983 |
| collision-induced dissociative chemical cross-linking reagents and methodology: applications to protein structural characterization using tandem mass spectrometry analysis. | chemical cross-linking combined with mass spectrometry is a viable approach to study the low-resolution structure of protein and protein complexes. however, unambiguous identification of the residues involved in a cross-link remains analytically challenging. to enable a more effective analysis across various ms platforms, we have developed a novel set of collision-induced dissociative cross-linking reagents and methodology for chemical cross-linking experiments using tandem mass spectrometry (ci ... | 2006 | 17134140 |
| molecular cloning and characterization of a major egg antigen in paragonimus westermani and its use in elisa for the immunodiagnosis of paragonimiasis. | a recombinant protein of a paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of p. westermani infection. the p. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5' methionine to the 3' stop codon. the predicted amino acid sequence of this egg antigen was 40, 38, and was 35% identical to heat shock proteins from schistosoma japonicum, schistosoma mansoni, and taenia saginata. the dis ... | 2007 | 17136389 |
| treatment and reinfection of water buffaloes and cattle infected with schistosoma japonicum in yangtze river valley, anhui province, china. | the aim of the present study was to evaluate the efficacy of praziquantel treatment of schistosoma japonicum infections in cattle and water buffaloes and to assess the natural rate of reinfection after treatment. the studies were conducted on 2 islands in the yangtze river, anhui province, china, from march 2003 to january 2004. the efficacy of praziquantel was 97% when applied orally wrapped in tree leaves at the recommended doses. the efficacy was measured using a miracidium hatching technique ... | 2006 | 17152955 |
| impacts of dietary intake and helminth infection on diversity in growth among schoolchildren in rural south china: a four-year longitudinal study. | the authors carried out six transverse anthropometric, dietary, and parasitological surveys of 274 schoolchildren (138 boys and 136 girls), aged 10-12 years (in 2001), from the dongting lake region in rural south china from 2001 to 2005 to examine intra-population differences in growth. compared to the chinese references, the subject children were judged to be retarded in growth at the population level. comparisons among tertiles classified by the extents of change in weight-for-age z score from ... | 2007 | 17160977 |
| studies on the protective immunity of schistosoma japonicum bivalent dna vaccine encoding sj23 and sj14. | in order to explore the high performance bivalent dna vaccine of schistosoma japonicum, the fatty-acid-binding protein (sj14) and the 23 kda transmembrane protein (sj23) two proteins were selected to construct the dna-based vaccine. it was successful to construct a bivalent dna vaccine using three strategies: the co-expression of two genes, a fusion gene expression and two kinds of plasmids in combination (cocktail vaccine). the bivalent dna was proven to express well in vitro and in vivo by ind ... | 2007 | 17182036 |
| comparison of the kato-katz technique, hatching test and indirect hemagglutination assay (iha) for the diagnosis of schistosoma japonicum infection in china. | the kato-katz technique (duplicate 41.7 mg fecal smears), hatching test and indirect hemagglutination assay (iha) were compared for their ability to detect human schistosoma japonicum infection in two endemic villages (zhonjiang and zhuxi) in rural china. the hatching test (using a nylon bag, and based on about 30 g of feces) and iha are conventional chinese diagnostic methods. in both villages, the trends of prevalences with age and sex were comparable for the different methods. in zhuxi, kato- ... | 2007 | 17188018 |
| th2 cytokines are associated with persistent hepatic fibrosis in human schistosoma japonicum infection. | we conducted a prospective cohort study in leyte, the philippines, among 611 schistosoma japonicum-infected participants 7-30 years old, all of whom were treated with praziquantel at baseline. to detect hepatic fibrosis, abdominal ultrasound was performed at baseline and 12 months after treatment. stool for assessment of s. japonicum infection was collected at baseline and at 3, 6, 9, and 12 months after treatment. cytokines (interleukin [il]-4, il-5, il-10, il-13, tumor necrosis factor- alpha , ... | 2007 | 17191174 |
| conservation of cd4+ t cell-dependent developmental mechanisms in the blood fluke pathogens of humans. | schistosoma blood flukes are trematode parasites with a cosmopolitan distribution that infect over 200 million people globally. we previously showed that schistosoma mansoni growth and development in the mammalian host is dependent on signals from host cd4+ t cells. to gain insight into the mechanisms that underlie this dependence, we sought to determine the evolutionary origins and limits of this aspect of the host-pathogen relationship. by infecting rag-1-/- mice with a range of different schi ... | 2007 | 17196594 |
| insight into the host-parasite interplay by proteomic study of host proteins copurified with the human parasite, schistosoma japonicum. | the tegument proteins of schistosome have attracted the most attention in studies of host-parasite interplay, while the host proteins acting at the host-parasite interface remained largely elusive. here, we undertook a high-throughput proteomic approach to characterize the schistosome-adsorbed host proteins. fifty five distinct host proteins were confidently identified in s. japonicum samples, including cercaria, schistosomula, adults, eggs, and miracidia, together with tegument and eggshell pre ... | 2007 | 17211827 |
| statistical evidence for glm regression parameters: a robust likelihood approach. | when a likelihood ratio is used to measure the strength of evidence for one hypothesis over another, its reliability (i.e. how often it produces misleading evidence) depends on the specification of the working model. when the working model happens to be the 'true' or 'correct' model, the probability of observing strong misleading evidence is low and controllable. but this is not necessarily the case when the working model is misspecified. royall and tsou (j. r. stat. soc., ser. b 2003; 65:391-40 ... | 2007 | 17211856 |
| vaccination against schistosoma japonicum infection by dna vaccine encoding sj22.7 antigen. | to observe the in vitro expression of dna vaccine pcdna3-sj22.7 and its immunological effect in mice, the recombinant plasmid pcdna3-sj22.7 was used to transfect hela cells with liposome-mediated method and the expression of sj22.7 mrna and protein was examined using reverse transcription-polymerase chain reaction, sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot. then, the ability of pcdna3-sj22.7 to protect against schistosoma japonicum challenge infections was analyze ... | 2007 | 17213956 |
| construction and expression of bivalent membrane-anchored dna vaccine encoding sjl4fabp and sj26gst genes. | in order to construct a eukaryotic co-expression plasmid containing membrane-anchored sjcl4fabp and sjc26gst genes and identify their expression in vitro, sj14 and sj26 genes were obtained by rt-pcr with total rna of schistosoma japonicum adult worms as the template and cloned into eukaryotic expression plasmid pvac to construct recombinant plasmids pvac-sj14 and pvac-sj26. then a 23 amino-acid signal peptide of human interleukin-2 (il-2) upstream sj14 or sj26 gene and a membrane-anchored sequen ... | 2006 | 17219949 |
| enzymatic and nonenzymatic synthesis of glutathione conjugates: application to the understanding of a parasite's defense system and alternative to the discovery of potent glutathione s-transferase inhibitors. | a primary pathway for metabolism of electrophilic compounds in schistosoma japonicum involves glutathione s-transferase (sjgst)-catalyzed formation of glutathione (gsh) conjugates. as part of a program aimed at gaining a better understanding of the defense system of parasites, a series of aromatic halides (1-8), aliphatic halides (9, 10), epoxides (11-20), alpha,beta-unsaturated esters (21, 22), and alpha,beta-unsaturated amides (23, 24) were prepared, and their participation in glutathione conj ... | 2007 | 17226963 |
| higher serum concentrations of dheas predict improved nutritional status in helminth-infected children, adolescents, and young adults in leyte, the philippines. | pubertal development and associated downmodulation of proinflammatory cytokines may predict improved nutritional status, independent of chronic parasite infections, in developing countries. we enrolled 731 individuals, aged 7-30 y, from leyte, the philippines, where helminth infections and nutritional morbidity are highly prevalent. the following data were collected: venous blood hemoglobin and serum concentrations of ferritin, dehydroepiandrosterone sulfate (dheas), c-reactive protein and proin ... | 2007 | 17237323 |
| synthesis of complex-type glycans derived from parasitic helminths. | chemical syntheses of complex-type glycans derived from the eggs of parasitic helminths, schistosoma mansoni and schistosoma japonicum were achieved. in addition, their analogs, which lack xylose and/or fucose residue(s), are described. these branched sugar chains were synthesized regio- and stereoselectively by using beta-mannosylation, desilylation under high-pressure and glycosylation in frozen solvent as key transformations. | 2007 | 17239358 |
| vaccines against the zoonotic trematodes schistosoma japonicum, fasciola hepatica and fasciola gigantica. | schistosoma japonicum, fasciola hepatica and f. gigantica are digenetic trematodes and, therefore, possess similar life cycles. while schistosomiasis japonica has for a long time been recognised as a major disease of both humans and animals, infection with fasciolids has only been considered of relevance to animals. however, a number of recent reports indicate that fasciolosis is becoming a serious public health problem, especially in south america, egypt and iran (sporadic cases are also on the ... | 2006 | 17274848 |
| in vitro and in vivo activities of synthetic trioxolanes against major human schistosome species. | schistosomiasis is a parasitic disease that remains of considerable public health significance in tropical and subtropical environments. since the mainstay of schistosomiasis control is chemotherapy with a single drug, praziquantel, drug resistance is a concern. here, we present new data on the antischistosomal properties of representative synthetic 1,2,4-trioxolanes (ozs). exposure of adult schistosoma mansoni for 24 h to a medium containing 20 mug/ml oz209 reduced worm motor activity, induced ... | 2007 | 17283188 |
| aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner. | we examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of schistosoma japonicum and s. mansoni. activity ascribable to aspartic protease(s) from s. japonicum and s. mansoni cleaved human hemoglobin. in addition, aspartic protease activities from s. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of s. mansoni. these findings support ... | 2007 | 17294005 |
| antibody isotype responses to paramyosin, a vaccine candidate for schistosomiasis, and their correlations with resistance and fibrosis in patients infected with schistosoma japonicum in leyte, the philippines. | we examined whether antibody isotype responses to paramyosin (pm), a vaccine candidate for schistosomiasis, are associated with age-dependent resistance and pathology in liver fibrosis using human sera collected from 139 individuals infected with schistosoma japonicum in leyte, the philippines. we report that iga and igg3 responses to pm showed a positive correlation with age and that the epitopes responsible were localized predominantly within the n-terminal half of pm. in addition, the igg3 re ... | 2007 | 17297052 |
| associations between specific antibody responses and resistance to reinfection in a senegalese population recently exposed to schistosoma mansoni. | we examined associations between schistosome-specific antibody responses and reinfection in senegalese individuals recently exposed to schistosoma mansoni. the effects of treatment, age, intensity of infection and duration of exposure on schistosome-specific antibody responses were also investigated by comparing immune responses in individuals exposed for less than 3 years with responses in people exposed for more than 8 years. all individuals were bled before treatment as well as 6 and 12 weeks ... | 2007 | 17313515 |
| integrating transcriptome, proteome and glycome analyses of schistosoma biology. | publication of the transcriptomes of schistosoma mansoni and schistosoma japonicum, in conjunction with the sequencing and assembly of their genomes, has generated a comprehensive picture of schistosoma transcriptional and genomic diversity. subsequently, researchers who study conjugal and developmental biology, tegumental composition and larval or egg, secretory and excretory products have used these data, in combination with the latest '-omics' technologies, to extend large-scale screens of th ... | 2007 | 17336161 |
| schistosoma mansoni: expression of fes-like tyrosine kinase smfes in the tegument and terebratorium suggests its involvement in host penetration. | protein tyrosine kinases (ptks) are important molecules in intra- and inter-cellular communication, playing a major role in signal transduction processes. we have previously identified and characterized the molecular structure of a new ptk in schistosoma mansoni, smfes. smfes exhibits the characteristic features of fes/fps protein tyrosine kinase subfamily of which it is the first member described in helminths. herein, we show that genes orthologous to smfes are also present in other schistosoma ... | 2007 | 17350619 |
| radiology corner. answer to last month's radiology case and images: schistosomiasis japonicum involving the liver and colon. | abdominal radiography showed subtle curvilinear densities overlying the left lower quadrant and central within the pelvis (fig. 1a). this was confirmed on intravenous contrast-enhanced abdominopelvic ct to be caused by thin mucosal surface calcifications within the descending and sigmoid colons (fig. 1b, c). incidental note is made of thin right hepatic lobe capsule calcifications (fig. 1d). these colonic and hepatic calcifications are characteristic of schistosomiasis infection of the gastroint ... | 2007 | 17357784 |
| [immune response and immunopathology in inducible costimulatory molecule (icos) transgenic mice infected with schistosoma japonicum]. | to establish the icos transgenic mice schistosomiasis japonica model and to observe the immune response and immunopathology of the model. | 2006 | 17361814 |
| [cloning, expression and phylogenetic analysis of schistosoma japonicum calcyphosine gene]. | to clone and express schistosoma japonicum (sj) calcyphosine gene, and purify the expressed protein. | 2006 | 17361818 |
| [re-understanding of liver cirrhosis induced by schistosomiasis japonica]. | whether the hepatic pipestem fibrosis induced by schistosomiasis japonica can result in cirrhosis, confusion exists among parasitologists in china. evidence from national and international pathologists and clinicians confirmed that the pipestem fibrosis could develop into cirrhosis undoubtedly. owing to different pathogenic causes, the characters of cirrhosis are different. to re-understand cirrhosis induced by schistosomiasis japonica is of significance for the diagnosis and treatment of the ad ... | 2006 | 17361823 |
| [construction of sjcb2 dna vaccine and its expression in hela cells]. | a recombinant plasmid containing cathepsin b endopeptidase of schistosoma japonicum (sjcb2) was constructed, indentified by pcr, restrictive enzyme, digestion and dna sequencing, and expressed into mammalian cells. immunochemistry examination showed that the sjcb2 gene can be expressed in the eukaryotic system, providing a basis for the development of schistosome dna vaccine. | 2006 | 17361824 |
| [biological identification on sub-cultivation cells of schistosoma japonicum adult worms in vitro]. | cultivation of cells from 30-day old schistosoma japonicum (s.j) adult worms showed that the growth features of the cells were semi-floating and accumulative. the survival rate of the primary cells, passage cells prior to the 5th generation and recovered cells was all up to 90%. phases of cell division were observed during cultivation. chromosome karyotype of the 5th generation cells possessed diploid feature of the blood-flukes (2n=8 in number). ultrastructure of the 5th generation cells showed ... | 2006 | 17361829 |
| [protective effects of co-immunization with sjctpi-hsp70 and interleukin-12 dna vaccines against schistosoma japonicum challenge infection in water buffalo]. | to induce protective effect of co-immunization with s. japonicum triose-phosphate isomerase fused to heat shock protein 70 (sjctpi-hsp70) plasmid and interleukin-12 (il-12) dna vaccines against schistosoma japonicum (chinese strain) infection in water buffalo. | 2006 | 17366973 |
| [studies on the protective effect of the mutant of sj23 dna vaccine against schistosomiasis]. | to investigate the protective immunity of the vaccine against schistosomiasis, a mutant of mr 23 000 membrane protein dna (sj23dna) without the homologous sequence of me491. | 2006 | 17366974 |
| [dna vaccine encoding sjir3 induces partial immune protection against schistosoma japonicum in mice]. | to detect the protection in mice induced by dna vaccine encoding sjir3 against schistosoma japonicum (sj). | 2006 | 17366976 |
| [a novel synthesized schistosoma japonicum miracidium attractant imprinted polymer]. | a novel molecularly imprinted polymer with adequate attractability for schistosoma japonicum miracidia was prepared. when adulterated with polyvinyl alcohol (pva), the fabricated film with good swelling property was formed which can suspend on water and slowly release xf (a chemical to be published). this reusable film can well attract schistosoma japonicum miracidia, and hopefully be used in the prevention of schistosome infection. | 2006 | 17366988 |
| familial aggregation of human susceptibility to co- and multiple helminth infections in a population from the poyang lake region, china. | human helminthiases are common in china, especially in rural areas where sanitation conditions are poor. co- and multiple infections with helminths are strikingly frequent. a cross-sectional parasitological and questionnaire survey was carried out in a population of 3205 individuals belonging to 498 families from five villages in the poyang lake region, jiangxi province, china, to assess their helminth infection status and to collect information on risk factors for infection. the prevalences for ... | 2007 | 17407778 |
| [bioinformatic identification and analysis of schistosoma japonicum adenine phosphoribosyltransferase]. | to identify adenine phosphoribosyltransferases in schistosoma japonicum and analyze their structural features. | 2007 | 17425969 |
| schistosoma japonicum eggs modulate the activity of cd4+ cd25+ tregs and prevent development of colitis in mice. | crohn's disease (cd) is considered to be caused by a disorder of the immune system and helminth infections may interact with development of the disease. we induced colitis in mice by trinitrobenzenesulfonic acid (tnbs) and observed the effects of intraperitoneally injected eggs of schistosoma japonicum on the course of the disease. the inflammation in the colon was reduced in egg-treated mice and secretion of ifn-gamma (a th1 cytokine) by cultured spleen cells in vitro was greatly suppressed, an ... | 2007 | 17433300 |
| environmental effects on parasitic disease transmission exemplified by schistosomiasis in western china. | environmental effects on the transmission of many parasitic diseases are well recognized, but the role of specific factors like climate and agricultural practices in modulating transmission is seldom characterized quantitatively. based on studies of schistosoma japonicum transmission in irrigated agricultural environments in western china, a mathematical model was used to quantify environmental impacts on transmission intensity. the model was calibrated by using field data from intervention stud ... | 2007 | 17438266 |
| cloning, expression and protective immunity evaluation of the full-length cdna encoding succinate dehydrogenase iron-sulfur protein of schistosoma japonicum. | 1071-bp fragment was obtained from the schistosoma japonicum (chinese strain) adult cdna library after the 3' and 5' ends of the incomplete expression sequence tag (est) of succinate dehydrogenase iron-sulfur protein of schistosoma japonicum (sjsdisp) were amplified by the anchored pcr with 2 pairs of primers designed according to the est of sjsdisp and the sequence of multiclone sites of the library vector. sequence analysis indicated that the fragment was a full-length cdna with a complete ope ... | 2007 | 17447029 |
| hepatic changes in congenital schistosoma japonicum infections in pigs. | the inflammatory response in liver tissue from piglets congenitally infected with schistosoma japonicum was examined at two different timepoints after infection. the piglets, which were the offspring of three sows infected with 9000 s. japonicum cercariae in the 10th week of gestation, were allocated into two groups (n=9 and 17) killed 5 or 11 weeks after birth, respectively. all piglets developed a low level infection,with no significant difference between the groups. inflammatory lesions in th ... | 2007 | 17459408 |
| novel sine families from salmons validate parahucho (salmonidae) as a distinct genus and give evidence that sines can incorporate line-related 3'-tails of other sines. | short interspersed elements (sines) constitute a group of retroposons propagating in the genome via a mechanism of reverse transcription, in which they depend on the enzymatic machinery of long retroposons (lines). over 70 sine families have been described to date from the genomes of various eukaryotes. here, we characterize two novel sines from salmons (actinopterygii: salmonoidei). the first family, termed slmi, was shown to be widespread among all genera of the suborder. these sines have a tr ... | 2007 | 17470437 |
| molecular cloning and identification of a novel clonorchis sinensis gene encoding a tegumental protein. | the tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. a cdna encoding a novel tegumental protein 20.8 kda (tp20.8) was found by large-scale sequencing of a clonorchis sinensis cdna library. this new cdna was 755 bp long containing an open reading frame of 555 bp, which encoded a 20.8-kda protein with an isoelectric point of 4.33. the deduced amino acid sequence exhibits 40 and 37% identity to schistosoma japonicum s ... | 2007 | 17476530 |
| efficacy and safety of drug combinations in the treatment of schistosomiasis, soil-transmitted helminthiasis, lymphatic filariasis and onchocerciasis. | this review concerns the efficacy and safety of combinations of various drugs, including albendazole (alb), diethylcarbamazine (dec), ivermectin (ivm), mebendazole and praziquantel (pzq). there were no significant pharmacokinetic interactions when alb-pzq, alb-dec, alb-ivm or alb-ivm-pzq were co-administered. alb did not add to the cure rate of pzq in the treatment of schistosoma japonicum, s. mansoni and s. haematobium. alb and dec in combination and alone were ineffective against s. haematobiu ... | 2007 | 17481681 |
| clonorchis sinensis: expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-lpap homologue. | from a clonorchis sinensis adult cdna plasmid library, a cdna clone encoding a novel lysophosphatidic acid phosphatase (lpap) homologue was isolated. the predicted molecular weight of putative protein was 48.8 kda and the deduced amino acid sequence had 45%, 32%, and 29% identity with lpap of schistosoma japonicum, danio rerio, and homo sapiens, respectively. prediction of signal peptide and western blot analysis indicated that the cslpap homologue was an excretory-secretory antigen (es antigen) ... | 2007 | 17507009 |
| use of a 44k oligoarray to explore the transcriptome of schistosoma mansoni adult worms. | recent advances in the study of schistosoma mansoni genome and transcriptome have led to a better description of the s. mansoni gene complement. in this work, we report the design and use of a new s. mansoni 60-mer oligonucleotide microarray platform with approximately 44,000 probes, based on all publicly available cdna sequence data for s. mansoni and schistosoma japonicum. the large number of probes combined with the extensive sequence annotation available allowed a comprehensive approach, whe ... | 2007 | 17517391 |
| schistosoma mekongi and schistosoma japonicum: differences in the distribution of eggs in the viscera of mice. | the difference in the distribution of schistosoma eggs in the viscera has not been clearly elucidated in the two closely related species schistosoma japonicum and schistosoma mekongi. in this study, we quantitatively compared the distribution of eggs in mice infected with the two species. in s. mekongi-infected mice, 56.6% to 69.4% of total eggs were found in the distal small intestine 9 to 15 weeks after infection, while in s. japonicum-infected mice, 48.8% to 71.8% of eggs were found in the pr ... | 2007 | 17521955 |
| paeoniflorin inhibits tgf-beta1-mediated collagen production by schistosoma japonicum soluble egg antigen in vitro. | the main pathological characteristics of hepatic fibrosis in schistosomiasis are the proliferation of hepatic stellate cells (hscs) and the deposition of collagen type i (col i) and collagen type iii (col iii). transforming growth factor beta-1 (tgf-beta1) plays an important role in hepatic fibrosis. paeoniflorin (pae) has been reported to have immunoregulatory effects; however, the mechanism of its anti-hepatic fibrosis in s. japonicum has not been elucidated. in the present study, we found tha ... | 2007 | 17524166 |
| amino acid transport in schistosomes: characterization of the permeaseheavy chain sprm1hc. | schistosomes are human parasitic flatworms that constitute an important public health problem globally. adult parasites live in the bloodstream where they import nutrients such as amino acids across their body surface (the tegument). one amino acid transporter, schistosome permease 1 light chain, sprm1lc, a member of the glycoprotein-associated family of transporters (gpaat), has been characterized in schistosomes. only a single member of the slc3 family of glycoproteins that associate with gpaa ... | 2007 | 17545149 |