Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacillus cereus-induced myonecrosis. | a patient with an incomplete amputation due to a crush injury to his arm developed a myonecrosis with gram-positive rods noted on muscle and wound aspirates. the patient was treated for a probable clostridium perfringens infection but culture results proved the organism to be bacillus cereus. in light of the reported resistance of bacillus cereus to penicillin, this case serves to emphasize the importance of expanded empiric coverage with high-dose penicillin and an aminoglycoside pending the re ... | 1984 | 6423833 |
| beta-lactamase-catalyzed hydrolysis of acyclic depsipeptides and acyl transfer to specific amino acid acceptors. | beta-lactamases from all three classes, a, b, and c, catalyze the hydrolysis of specific acyclic depsipeptide (phch2conhcr1r2co2chr3co2h) analogs of acyl-d-alanyl-d-alanine peptides. the depsipeptides investigated, which are chemically as reactive toward nucleophiles as penicillins, are in general poor substrates, although differences between the classes of beta-lactamases have been observed: the order of effectiveness seems to be c greater than b greater than a. certain class a and c beta-lacta ... | 1984 | 6424114 |
| toxigenic bacillus cereus var. fluorescens in human milk. | human milk with high bacterial load of b. cereus var. fluorescens is reported. the organism was identified by morphological, cultural, and biochemical characteristics. lecithinase and haemolysin were produced in rabbit and bovine blood. the toxigenic nature of the organism was confirmed by production of necrosis on rabbit skin and fluid accumulation in rabbit ileal loop. an antibiotic resistogram also has been included. | 1984 | 6425376 |
| [comparative studies of the antibacterial activity of procaine powder of various origins]. | 1983 | 6426028 | |
| entomopathogenic spore-formers from soil samples of mosquito habitats in northern nigeria. | spore-forming bacteria, isolated from bottom soil samples of periodical mosquito-breeding places in kaduna, n. nigeria, were tested for mosquito pathogenicity. culex pipiens autogenicus was used for this screening. five strains, all of the bacillus thuringiensis group, were active in 85 isolates from 32 samples. the isolates were from three different serotypes (h-14, h-5a5c, and h-8a8b). the activity, compared with the sip standard, was in the known limits of b. thuringiensis israelensis. | 1984 | 6426190 |
| heat-induced temperature sensitivity of outgrowing bacillus cereus spores. | inactivation of bacillus cereus spores during cooling (10 degrees c/h) from 90 degrees c occurred in two phases. one phase occurred during cooling from 90 to 80 degrees c; the second occurred during cooling from 46 to 38 degrees c. in contrast, no inactivation occurred when spores were cooled from a maximum temperature of 80 degrees c. inactivation of spores at a constant temperature of 45 degrees c was induced by initial heat treatments from 80 to 90 degrees c. the higher temperatures accelerat ... | 1984 | 6426390 |
| bacillus cereus enterotoxins: present status. | 1984 | 6427037 | |
| diprotins a and b, inhibitors of dipeptidyl aminopeptidase iv, produced by bacteria. | 1984 | 6427168 | |
| occurrence of bacillus cereus in milk and milk products in egypt. | the presence of b. cereus spores in raw, pasteurized, and dry milk as well as in damietta and processed cheese and in a baby food product was investigated, using three selective agar media for enumeration. the organism was detected in 70, 90, and 50% of milk, in processed and damietta cheese samples, respectively. spore counts varied from 10(2) to 10(6) and from 10 to 10(2)/ml of raw and pasteurized milk, respectively. in the two types of cheese and dried milk spore densities reached 10 to 10(3) ... | 1984 | 6428076 |
| factors affecting germination and growth of bacillus cereus spores in milk. | the effect of salt concentration, acidity, and storage temperature on the spore germination of bacillus cereus and the growth rate of the organism was investigated. increasing the amount of salt to more than 5% or acidifying the milk to ph 5.0 or 4.5 greatly affected the germination and growth of b. cereus spores. when a culture of streptococcus lactis was used as an acidifying agent, b. cereus showed a normal growth rate during the first 12 h, followed by a sharpe reduction in numbers. when b. ... | 1984 | 6428077 |
| glycosidase activities of bacillus anthracis. | bacillus anthracis could be distinguished from the taxonomically related species b. cereus, b. mycoides, and b. thuringiensis by a comparison of glycosidase activities. all the bacilli tested possessed alpha-glucosidase activity, as evidenced by the hydrolysis of p-nitrophenyl-alpha-d-glucoside. in b. anthracis, the glucosidase activity could be enhanced by the addition of agents which damage cellular surface structures. treatment of b. anthracis strains with toluene. triton x-100, or mutanolysi ... | 1984 | 6429187 |
| bacillus cereus as agent of peritonitis during peritoneal dialysis. | 1984 | 6429559 | |
| [bacillus cereus infection in newborn infants]. | 1984 | 6429745 | |
| bacillus cereus meningitis and bacteremia associated with an ommaya reservoir in a patient with lymphoma. | after placement of an ommaya reservoir, meningitis and bacteremia due to bacillus cereus occurred in a patient with stage iv lymphoblastic lymphoma and meningeal involvement. bacillus species have been implicated as meningeal pathogens after lumbar punctures. these organisms have become an important cause of severe infection, especially in immunologically compromised patients. | 1984 | 6429866 |
| [medical aspects of elaboration of microbiological criteria for evaluating the quality and principles of microbiological standards of dried milk and other mixtures for children in the first year of life]. | the author demonstrates the ability of st. aureus, e. coli o iii, b. cereus and other opportunistic microorganisms to multiply in model media similar to those in the alimentary tract of children of the first year of life. describes the principal differences between microbiological criteria of evaluating the quality and safety of infant food mixtures and other products intended for children of the first year of life and these criteria for consumer goods. emphasizes that food mixtures for infant n ... | 1984 | 6429954 |
| rapid assay for tetracycline in premixes and mixed feeds. | a rapid assay for tetracyclines in premixes and mixed feeds is described, which uses the extraction and dilution systems of aoac methods, and a strain of bacillus stearothermophilus atcc 12980 selected to grow at 40 degrees c. the incubation period is 4.5 h. the rapid assay yields results similar to those obtained using the aoac methods. for a 50 g chlortetracycline (ctc)/lb commercial premix, the rapid procedure averaged 109.2% of label vs 104.4% obtained using the aoac method; for a 20 g ctc/l ... | 1984 | 6430864 |
| [bacillus cereus: a wound contaminant but also a causative agent of severe general infections?]. | 1984 | 6431304 | |
| transitory uv resistance during germination of uv-sensitive spores produced by a mutant of bacillus cereus 569. | 1984 | 6431457 | |
| [the hygienic significance of aerobic sporulating microbes]. | 1984 | 6431683 | |
| enzyme-linked lectinosorbent assay (ella) for detecting bacillus anthracis. | an enzyme-linked lectinosorbent assay (ella) has been developed for the rapid identification of bacillus anthracis. using two different lectin-conjugates, the ella test can differentiate bacillus anthracis from closely related species, such as bacillus cereus and bacillus cereus var. mycoides, in approximately two hours. in addition to having high specificity, the test can also detect small numbers of the bacterium. | 1984 | 6432529 |
| the binding and hydrolysis of sphingomyelin by phospholipase c (bacillus cereus) as shown by 31p nmr. | 31p nuclear magnetic resonance studies showed that heavily inactivated phospholipase c (bacillus cereus) initially caused line broadening in the 31p resonance from sphingomyelin thus indicating enzyme-lipid association. using larger amounts of enzyme or longer preincubation caused a displacement of the 31p resonance to a position suggesting phosphorylcholine formation. incubation of the heavily inactivated enzyme with a phosphonolipid caused a displacement of the 31p resonance suggesting hydroly ... | 1984 | 6432601 |
| prumycin produced by bacillus cereus. | 1983 | 6432762 | |
| stimulation of germination of unactivated bacillus cereus spores by ammonia. | inclusion of ammonia in germinant mixtures containing l-alanine and inosine stimulated germination of unactivated bacillus cereus spores at rates equal to those obtained using heat-activated spores without ammonia. d-alanine had little effect on germination of heat-activated spores, but severely inhibited germination of unactivated spores in the presence of ammonia. ammonia did not replace the requirement for either l-alanine or inosine: all three compounds were required for rapid germination. k ... | 1984 | 6432943 |
| identification of bacillus strains using the api system. | a system is described for the rapid and accurate identification of bacillus isolates using a matrix of results from tests in the api 20e and api 50chb strips and from supplementary tests. api system tests have been shown to be more reproducible than the classical tests. a taxonomy based upon api tests is in good agreement with those obtained by other methods. the results matrix can also be used in computer assisted identification. | 1984 | 6432953 |
| [incidence of bacillus cereus and various pathogenic microorganisms in food for infants and small children]. | 206 samples of commercially available infant food and their ingredients were examined for the content of b. cereus and coagulase-positive staphylococci as well as for the presence of salmonellae. b. cereus could be isolated from 64 samples with numbers between 3 and 460/g (only 2 samples greater than 100/g). two samples contained coagulase-positive staphylococci (4/g each), and no sample salmonellae. growth of b. cereus in reconstituted samples with counts of less than or equal to 100/g incubate ... | 1984 | 6433594 |
| [growth of test microorganisms used in determining antibiotic activity on synthetic nutrient media]. | the growth of gram-negative sporulating bacteria and yeast-like fungi used in a microbilogical assay of antibiotic activity was studied on solid synthetic media of simple composition. their reproduction with the microbial growth of different density was shown to be possible on media containing available and strictly standardized components, such as salts, glucose and disubstituted sodium phosphate. the cultures tested were not similar by their growth requirements. | 1984 | 6433784 |
| evidence for an arene oxide-nih shift pathway in the transformation of naphthalene to 1-naphthol by bacillus cereus. | bacillus cereus atcc 14579 transformed naphthalene predominately to 1-naphthol. experiments with [14c]naphthalene showed that over a 24 h period, b. cereus oxidized 5.2% of the added naphthalene. 1-naphthol accounted for approximately 80% of the total metabolites. b. cereus incubated with naphthalene under the presence of 18o2 led to the isolation of 1-naphthol that contained 94% 18o. the metabolism of [1-2h]- and [2-2h]-naphthalene by b. cereus yielded 1-naphthol which retained 95% and 94% deut ... | 1984 | 6433850 |
| effect of phospholipase c (bacillus cereus) on freshly isolated and 4-day-stored human platelets. | phospholipase c (from bacillus cereus) was used to study fresh and stored human platelets. provided that the enzyme was inactivated before lipid extraction, no significant degradation of phospholipid in fresh cells was noted, even when platelets were activated or induced to change shape by adp, collagen or thrombin. with platelets isolated from concentrates stored for transfusion for 4 days at 22 degrees c, membrane phospholipids were degraded by the enzyme to an extent depending on the ph in th ... | 1984 | 6433899 |
| an anion binding site in the active centre of phospholipase c from bacillus cereus. | the bi-zn2+-enzyme phospholipase c (bacillus cereus) is readilly inhibited by univalent anions. n.m.r. studies on the 113cd-substituted enzyme showed the presence of an inert and a perturbable metal, neither of which seemed affected by i-. x-ray crystallographic analysis showed the binding of one i- to the enzyme 4.8 a from the nearest metal (too far for a metal-halide bond). phospholipase c contains an arginine residue apparently necessary for substrate binding and i- partially protected agains ... | 1984 | 6433934 |
| a model for the secondary structure of beta-lactamases. | a 3-dimensional model, common for the secondary structures of four beta-lactamases obtained from escherichia coli, bacillus licheniformis, bacillus cereus and staphylococcus aureus, is proposed. the predictions of the structures were made by the hydrophobicity profiles method complemented by the modified chou and fasman's method. the model proposed presents 56% constancy and can be described as a 2-domain structure, in agreement with low resolution x-ray data reported for the e. coli enzyme. the ... | 1984 | 6434348 |
| salt and base sequence specific changes in the chiroptical properties of dna. | chiroptical properties of natural dna molecules differing in base composition were studied in solutions with high concentrations of monovalent sodium and caesium salts. it was found that the properties were dependent on the dna base sequence and nature of both cations and anions. a comparison with the behaviour of the synthetic molecules of dna demonstrated that the salt-induced changes in the natural molecules of dna could not be accounted for by the appearance of the left-handed z conformation ... | 1984 | 6434373 |
| bacillus cereus electron transport and proton motive force during aerotaxis. | aerotaxis (migration towards oxygen) of bacillus cereus m63, a motile strain, was inhibited by potassium cyanide and 2-heptyl-4-hydroxyquinoline n-oxide, indicating a requirement for both the terminal oxidase (cytochrome aa3) and the cytochrome b segment of the electron transport system. the concentration of oxygen that gave a half-maximal aerotactic response (k0.5) was 0.31 microm, which was similar to the km for respiration (0.80 microm). the proton motive force increased from -135 to -177 mv ... | 1984 | 6434511 |
| respiratory system of vegetative and sporulating bacillus cereus. | the composition and organization of the bacillus cereus respiratory system were studied. the abolition of nadh-dependent respiration in vegetative and sporulating cell membranes by near-uv light (360 nm) indicated that electrons reduce oxygen only through a quinone-cytochrome pathway. difference spectroscopy demonstrated the presence of cytochromes b555, c548, aa3, b562, and a2. this composition and studies with respiratory inhibitors suggested that cytochromes are organized in at least two bran ... | 1984 | 6434526 |
| immunologic profile of juvenile periodontitis. ii. neutrophil chemotaxis, phagocytosis and spore germination. | patients with juvenile periodontitis (jp) were grouped into one of the two recognized forms of the disease: a localized form affecting incisors and first molars (ljp) and a generalized form affecting more than 14 teeth (gjp). the role of the neutrophil in the etiology and pathogenesis of ljp has recently been recognized. experiments aimed at confirming previous information related to neutrophil chemotaxis defects in ljp were performed. additional experiments aimed at demonstrating significant di ... | 1984 | 6434725 |
| identification and characterization of some bacterial membrane sulfhydryl groups which are targets of bacteriostatic and antibiotic action. | covalent modification of sulfhydryl groups which become sensitive toward sulfhydryl agents during germination of bacillus cereus spores exerts a profound bacteriostatic effect, resulting in outgrowth inhibition. the modified spore components are membrane species of 13,000, 28,000, and 29,000 daltons. detergent disruption of the membrane inactivated the sulfhydryl groups. a highly sigmoid inhibition curve (n = 11.8) with diamide suggested the participation of closely neighboring sulfhydryl groups ... | 1984 | 6436249 |
| novel quantitative method for determination of molecular species of phospholipids and diglycerides. | a novel method is described for the quantitative analysis of subclasses (alk-1-enylacyl, alkylacyl, and diacyl types) and molecular species within each subclass of glycerophosphatides. diradylglycerols from phospholipase c hydrolysis of the phospholipids are converted to benzoate derivatives, the benzoates are separated into their respective subclasses by thin-layer chromatography, and quantitated by measuring absorbance at 230 nm. molecular species within individual subclasses are separated usi ... | 1984 | 6436283 |
| deoxyribose 1-phosphate: radioenzymatic and spectrophotometric assays. | a method has been developed to measure deoxyribose 1-phosphate in the presence of ribose 1-phosphate and other sugar phosphates. the specificity of the method is based on the observation that only deoxyribose 1-phosphate is hydrolyzed by heating at ph 7.4, while both deoxyribose 1-phosphate and ribose 1-phosphate remain unchanged when heated at ph 10. a tissue extract is heated at ph 10. the amount of deoxyribose 1-phosphate plus ribose 1-phosphate is determined from that of deoxyinosine plus in ... | 1984 | 6436359 |
| bacterial growth in artificial capillary spaces. | in experiments on the influence of microscopic capillaries on the growth of escherichia coli, brevibacterium linens, micrococcus flavus and bacillus cereus, a distinct delay in growth was observed. the difference in counts was greatest after 8 h. later a slight equilibration of counts was noted. with b. cereus, only slight or no spore formation was observed under microcapillary conditions. | 1984 | 6437860 |
| unusually severe food poisoning from vanilla slices. | thirty six people suffered from severe vomiting and diarrhoea 15 min to 3 h after eating vanilla slices from the same bakery. five patients were admitted to hospital, and one developed unusual skin lesions after admission. staphylococcus aureus was isolated in large numbers from vanilla slices of the same batch as those giving rise to symptoms, and from five faecal specimens obtained from affected persons. bacillus cereus and bacillus subtilis were also isolated from the slices. unbaked custard ... | 1984 | 6438231 |
| [an approach to estimation of internal ph of bacterial spores by 31p nuclear magnetic resonance]. | 1984 | 6438366 | |
| induction of deoxyribose-5-phosphate aldolase of bacillus cereus by deoxyribonucleosides. | in bacillus cereus purine ribonucleosides and deoxyribonucleosides share a common inducible catabolic pathway, leading to the formation of ribose-5-p or deoxyribose-5-p respectively inside the cell, while the purine ring remains in the external medium. both ribo- and deoxyribonucleosides are inducers of adenosine deaminase, inosine-guanosine phosphorylase and phosphopentomutase, the enzymes of the catabolic pathway. we now show that deoxyribonucleosides, but not ribonucleosides, induce the aldol ... | 1984 | 6439205 |
| an outbreak of bacillus cereus food poisoning in a school hostel, klang. | 1984 | 6439984 | |
| some microbiological aspects of inedible rendering processes. | various aspects of the bacteriology of inedible rendering have been investigated in order to establish a solid basis for future decisions concerning an up-to-date and flexible legislation on rendering. thermal death (td)-graphs for spores of b. cereus and cl. perfrigens, pa 3679 (fig. 3), and heat transmission equations for animal tissues have been determined. by using the heat transmission data for bones and the td graphs for the spores it is possible to predict the decimal reductions of spores ... | 1984 | 6441385 |
| mycocerein, a novel antifungal peptide antibiotic produced by bacillus cereus. | a peptide was obtained from culture filtrates of a bacterium which was newly isolated and tentatively named bacillus cereus sw. the peptide was composed of asx, ser, glx, leu, tyr, pro, and an unknown amino acid in a ratio of 2:1:1:1:1:1:1, but, unless hydrolyzed with hci, it was ninhydrin reaction negative. the peptide effectively inhibited the growth of all fungi and yeasts so far examined, whereas it inhibited none of the bacteria tested. | 1984 | 6441513 |
| [contamination, biotype and serotype of bacillus cereus in food]. | 1984 | 6441694 | |
| [microflora of sea shellfish]. | 1984 | 6442255 | |
| properties of neutrophils recruited into inflammatory foci with homologous or heterologous antigen in immunized ewes. | studies were undertaken to determine functional properties of neutrophils attracted into involuted mammary glands of staphylococcus aureus--immunized ewes by soluble antigens prepared from s. aureus (homologous antigen) or from bacillus cereus (heterologous antigen). the ewes were immunized with an s. aureus vaccine known to stimulate synthesis of cytophilic igg2 antibody, and the inflammatory responses were elicited 4-8 weeks later by infusing homologous antigen into one gland and heterologous ... | 1983 | 6442921 |
| [effect of benzoic and sorbic acids on bacillus cereus and torulopsis candida]. | 1984 | 6443046 | |
| analysis of biological thiols: derivatization with monobromotrimethylammoniobimane and characterization by electrophoresis and chromatography. | 1980 | 6449160 | |
| purification and characterization of a dna-dependent atpase from bacillus cereus. | a dna-dependent atpase (molecular weight 71 000) free of nuclease activity has been purified from bacillus cereus. the enzyme shows similar characteristics as the enzyme isolated from escherichia coli and bacillus subtilis. heat denatured dna stimulates the rate of atp hydrolysis to adp and pi to an extent about tenfold higher than the native dna. double stranded dna without single stranded regions is not a suitable cofactor for the enzyme. the atpase is inhibited by adenosine 5'-(beta, gamma-im ... | 1980 | 6451147 |
| enzymatic reduction of mercurous and mercuric ions in bacillus cereus. | a strain of bacillus cereus, which can grow in nutrient broth containing 50 microm hgcl2, was isolated from soil. mercurous or mercuric ion dependent oxidation of reduced nadph was demonstrated in crude extracts of cells grown in nutrient broth containing 10 microm hgcl2. the properties of this mercuric reductase were similar to those of the enzymes from r factor bearing escherichia coli in substrate specificity, heat stability, requirement of sulfhydryl compounds, sensitivity to some heavy meta ... | 1981 | 6452192 |
| separation of atp-dependent dnase to atpase and dnase. | a 250-fold purified atp-dependent dnase from bacillus cereus has been separated to dna-dependent atpase i and ii and a dnase specific for single-stranded dna (ssdnase) by means of high resolution of deae cellulose chromatography. simultaneously with the separation of atpase and ssdnase, a decrease in atp-dependent dnase activity was observed. complete separation resulted in the total loss of atp-dependent dnase activity. reconstitution of atp-stimulated dnase activity was dependent on the ratio ... | 1981 | 6456634 |
| procedure for assay of tobramycin in serum containing moxalactam. | moxalactam was examined as a substrate for 11 beta-lactamases. hydrolysis was shown only with the beta-lactamase from bacillus cereus, a nonpathogenic bacterium. tobramycin was assayed in tobramycin-moxalactam mixtures by using this beta-lactamase. | 1981 | 6457559 |
| thermal melting of poly(da-dt).poly(da-dt) in methanol-water solutions. | thermal melting of a synthetic dna poly(da-dt).poly(da-dt) was investigated in methanol-water solutions. methanol decreased melting temperature of the polynucleotide but the decrease was qualitatively different in the presence of milimolar concentrations of sodium and cesium cations. the difference is a consequence of the fact that poly(da-dt).poly(da-dt) undergoes a helix-helix conformational isomerization in methanol-water solutions which is cesium cation specific. the arising conformation is ... | 1984 | 6487316 |
| selective medium for isolation of clostridium butyricum from human feces. | a selective medium, clostridium butyricum isolation medium (bim), is described for the isolation of c. butyricum from human feces. the bim is a synthetic minimal medium and contains trimethoprim (16 micrograms/ml), d-cycloserine (10 micrograms/ml), and polymyxin b sulfate (20 micrograms/ml) as selective inhibitory agents. qualitative tests indicated that c. butyricum and other butyric acid-producing clostridia grew on bim, clostridium sphenoides and bacillus cereus produced small colonies, and o ... | 1984 | 6490827 |
| spectrophotometric and radioenzymatic determination of ribose-5-phosphate. | the present work describes an assay which is highly specific for ribose-5-phosphate. the method is based on the following three-stage enzymatic conversion: (1) ribose 5-phosphate in equilibrium ribose 1-phosphate (phosphopentomutase); (2) ribose 1-phosphate + adenine in equilibrium adenosine + pi (adenosine phosphorylase); (3) adenosine + h2o----inosine + nh3 (adenosine deaminase). ribose 5-phosphate may be determined either directly following the change in absorbance at 265 nm associated with t ... | 1984 | 6530507 |
| ovalbumin is an elastase substrate. | ovalbumin is partially homologous in sequence with the proteinase inhibitors alpha 1-proteinase inhibitor and anti-thrombin iii. the region of sequence in ovalbumin which corresponds to the reactive sites of these proteinase inhibitors is susceptible to attack by subtilisin, elastase, thermolysin, bromelain, and bacillus cereus protease. the esterase activity of elastase is not inhibited by ovalbumin, but ovalbumin is efficiently cleaved by elastase. in contrast with these proteases, trypsin doe ... | 1984 | 6568226 |
| monolithic tetracycline-containing fibers for controlled delivery to periodontal pockets. | for the purpose of developing controlled delivery devices for periodontal therapy which would release over several days, six fiber types made of tetracycline-loaded biocompatible polymers were manufactured and tested. polyethylene, polypropylene, polycaprolactone, polyurethane and cellulose acetate propionate all released their drug load within 1 day. ethylene vinyl acetate fibers, however, provided in vitro sustained release for periods up to 9 days. a bioassay was designed to measure levels of ... | 1983 | 6580409 |
| the ph-dependence of class b and class c beta-lactamases. | the classification by structure allots beta-lactamases to (at present) three classes, a, b and c. the ph-dependence of the kinetic parameters for class b and class c have been determined. they differ from each other and from class a beta-lactamases. the class b enzyme was beta-lactamase ii from bacillus cereus 569/h/9. the plots of kcat against ph for the hydrolysis of benzylpenicillin by zn(ii)-requiring beta-lactamase ii and co(ii)-requiring beta-lactamase ii were not symmetrical, but those of ... | 1983 | 6604522 |
| the coagulation factor vii in pregnancy. | the hypercoagulable state in pregnancy is partly caused by the increased activity of factor vii in plasma. we demonstrate here that this activity is reduced to levels similar to those in plasma from non-pregnant women by highly purified phospholipase c from bacillus cereus, i.e. the activity increase is due to a circulating complex of factor vii and a phospholipase c-sensitive compound. phospholipase c had no effect on the levels of factor ii and x in blood from pregnant women. this novel form o ... | 1984 | 6607066 |
| freeze-dried mixed cultures as samples for proficiency tests and collaborative studies in food microbiology. | a method is presented for preparing samples of freeze-dried mixtures of microorganisms for proficiency tests and collaborative studies. the samples may include most microorganisms that are found in routine analysis in food laboratories. transport of samples during 48 h did not decrease the number of microorganisms, nor was the variability among samples significantly affected by transport. the standard deviation of counts after 5 weeks of storage varied from 0.04 (staphylococcus aureus) to 0.17 ( ... | 1983 | 6643366 |
| antibacterially active substituted anilides of carboxylic and sulfonic acids. | anilides of carboxylic and sulfonic acids were prepared and tested for antimicrobial activity. while these anilides were ineffective against gram-negative organisms, there was a good correlation between chemical structure and biological activity against gram-positive species. both the nature and position of the benzene ring substituents and the length of the carbon side chain affected the activity and specificity of the compounds. the highest activity was observed when the acyl or sulfuryl moiet ... | 1983 | 6644743 |
| [microbiological degradation of glucosinolates in defatted rapeseed meal]. | during the degradation of thioglucosides in defatted rape seed meal (res) microorganisms were found, whose ability to degrade glucosinolates (gsl) and vinyl thio-oxazolidone (vto) was not known so far. the isolated microorganisms are two strains of bacteria of the species bacillus cereus and the yeast trichosporon cutaneum. the degradation of gsl and vto in the cultural broths by the bacillus cereus strains was the more complete the more other gram-negative bacteria from res were additionally pr ... | 1983 | 6684210 |
| phospholipids chiral at phosphorus. preparation and spectral properties of chiral thiophospholipids. | the thiophospholipid 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) was shown to be a mixture of two diastereomers by 31p nuclear magnetic resonance. the isomer that resonates at the lower field in cdcl3 (56.12 ppm) was designated as isomer a and the other (resonates at 56.07 ppm) as isomer b. phospholipase a2 from four different sources (bee venom, naja naja venom, crotalus adamanteus venom, and porcine pancreas) was shown to hydrolyze the isomer b of dppsc specifically, whereas phosph ... | 1983 | 6688028 |
| regulation of protoxin synthesis in bacillus thuringiensis. | a derivative of bacillus thuringiensis subsp. kurstaki (hd-1) formed parasporal inclusions at 25 degrees c, but not at 32 degrees c. this strain differed from the parent only in the loss of a 110-megadalton (md) plasmid, but plasmid and chromosomal copies of protoxin genes were present in both strains. on the basis of temperature shift experiments, the sensitive period appeared to be during midexponential growth, long before the time of protoxin synthesis at 3 to 4 h after the end of exponential ... | 1984 | 6725205 |
| postoperative neurosurgical infections due to bacillus species. | the cases of 2 patients with postoperative ventriculitis due to bacillus species bacteria are presented. bacillus licheniformis was isolated from one patient following removal of an intraventricular meningioma, and bacillus cereus from another patient following placement of a ventriculoperitoneal shunt. both isolates were resistant to a variety of antibiotics, but both were sensitive to gentamicin and chloramphenicol. these cases emphasize several points; (a) bacillus species, usually thought to ... | 1982 | 6758158 |
| phenylpropynal, a specific, irreversible, non-beta-lactam inhibitor of beta-lactamases. | phenylpropynal is a specific, irreversible, non-beta-lactam inhibitor of typical beta-lactamases. in the presence of millimolar concentrations of phenylpropynal, the beta-lactamase i of bacillus cereus and the beta-lactamases of staphylococcus aureus and escherichia coli become completely inactivated; the beta-lactamase ii of b. cereus is not affected. the e. coli beta-lactamase is considerably more sensitive to the reagent than the gram-positive enzymes. a variety of structural analogs of pheny ... | 1980 | 6765945 |
| atp-sensitive ribonuclease of bacillus cereus. | 1980 | 6766142 | |
| studies on the heat resistance of bacillus cereus spores and growth of the organism in boiled rice. | a comparison was made of the heat resistance of bacillus cereus spores at 95 degrees c. spores of serotype 1 strains were more resistant than those of the other types tested. however, there was little difference in the growth rate of the various serotypes in boiled rice at 22 degrees c. most samples of uncooked rice contained multiple serotypes of b. cereus. these results indicate that the cooking procedure used for the preparation of cooked rice is likely to be selective for certain serotypes, ... | 1980 | 6766156 |
| bacillus cereus autolytic endoglucosaminidase active on cell wall peptidoglycan with n-unsubstituted glucosamine residues. | an autolytic glycosidase from a lysozyme-resistant strain of bacillus cereus capable of cleaving the glycosidic linkages of n-unsubstituted glucosamine in the cell wall peptidoglycan was studied. this glycosidase activity, together with n-acetylmuramyl-l-alanine amidase activity, was found in an autolytic enzyme preparation obtained from the 20,000 x g precipitate fraction by means of autolysis followed by ammonium sulfate fractionation. the major saccharide fragments resulting from digestion of ... | 1980 | 6766437 |
| the crystalline layers in spores of bacillus cereus and bacillus thuringiensis studied by freeze-etching and high resolution electron microscopy. | the comparative morphology of spores from bacillus cereus, b. thuringiensis s-9 (wildtype), b. thuringiensis hb 9-1 (acrystaliferous mutant) and b. finitimus, was studied by the freeze-etching technique. particular attention was given to the three crystalline layers found in these spores, the pitted layer, the parasporal layer, and the basal layer of the exosporium with lattice constants of 9.2 nm, 5.8 nm and 8.0 nm respectively. the parasporal layers, corresponding to the fraction of fl of sche ... | 1980 | 6766865 |
| meningitis and bacteremia; induced by bacillus cereus. | 1980 | 6767208 | |
| panophthalmitis due to bacillus cereus. | a rapidly progressing panophthalmitis due to bacillus cereus developed in three patients. infection was associated with intravenous drug abuse in two patients and was traced to contaminated injection paraphernalia in one. in the third patient, infection was associated with a foreign-body injury to the eye. anterior chamber aspiration revealed the organism on gram's stain in one case and isolation of the bacteria in all three. despite intravenous and intraocular antibiotic therapy, the infection ... | 1980 | 6767460 |
| a bacillus cereus mutant defective in spore coat deposition. | spores of bacillus cereus mutants selected for slow response to germinants and sensitivity to lysozyme were found to be deficient in coat, but were heat-resistant and contained the same quantity of dipicolinic acid as the wild-type. while the average coat protein content of the spores was 25% of the wild-type, many spores were coatless with large whorls of coat deposited in the cytoplasm. these coat deposits were isolated in renografin gradients and found to cross-react immunologically with wild ... | 1980 | 6767805 |
| plasmid-mediated transformation in bacillus megaterium. | a transformation system was developed for bacillus megaterium by using antibiotic resistance plasmid deoxyribonucleic acid molecules derived from staphylococcus aureus and bacillus cereus. lysozyme-generated protoplasts of b. megaterium allowed uptake of plasmid deoxyribonucleic acid in the presence of polyethylene glycol. transformants expressed the antibiotic resistance determinants present on the plasmid deoxyribonucleic acid, and reisolated plasmid deoxyribonucleic acid yielded restriction e ... | 1980 | 6769902 |
| rapid isolation of bacteria from septicemic patients by use of an antimicrobial agent removal device. | the new antimicrobial removal device increased the efficacy of conventional methods for isolating bacteria from the blood of septicemic patients. the device removes as much as 100 microgram of antibiotics per ml from whole blood without a significant decrease in bacteria. of 51 patients studied, 31 yielded positive cultures, and the antimicrobial removal device permitted earlier isolation of the causative agent from blood. subcultured within the first 12 h yielded 12 positive isolates, as the 31 ... | 1980 | 6769953 |
| bacillus cereus infections. | 1980 | 6769974 | |
| [aerobic sporiferous bacteria as causative agents of inflammation]. | 1980 | 6770318 | |
| the isoelectric point of phospholipase c from bacillus cereus. | 1980 | 6771157 | |
| the hydrolysis of sphingomyelin by phospholipase c from bacillus cereus. | 1980 | 6771160 | |
| delayed germination of bacillus cereus t spores after treatment with trichloroacetic acid and their reactivation by heating. | treatment of bacillus cereus t spores with trichloroacetic acid delayed their germination. the extent of retardation depended on the concentration of trichloroacetic acid, and the temperature, ph and duration of treatment. the effect was completely reversed by subsequent heating, and this restoration of germination also depended on the temperature and duration of heat treatment. fourteen compounds were examined for their ability to suppress germination of spores. the halogenated fatty acids test ... | 1980 | 6771492 |
| measurements of the ph within dormant and germinated bacterial spores. | the ph within the core or central region of dormant spores of bacillus cereus and b. megaterium is 6.3-6.4 irrespective of the external ph. however, the spore's internal ph rises to 7.3-7.5 upon germination. the low internal ph of the dormant spore may be a contributing factor to its metabolic dormancy. | 1980 | 6771755 |
| bacillus cereus bacteremia and hemolytic anemia in a patient with hemoglobin sc disease. | a patient with hemoglobin sc disease and cholelithiasis was found to have bacillus cereus bacteremia. hemolytic anemia developed, for which common causes of hemolysis were excluded, suggesting a relationship with the bacteremia. following in vitro incubation, type o erythrocytes were hemolyzed by the culture, but not by a bacteria-free filtrate. this case confirms the association between sickle cell disorders and cholelithiasis with b cereus infections. in addition, it provides evidence for in v ... | 1980 | 6772119 |
| certain monocyclic beta-lactams are beta-lactamase substrates: nocardicin a and desthiobenzylpenicillin. | 1980 | 6772177 | |
| evidence of thermonuclease production by bacillus spp. and enterococci in naturally contaminated cheese. | of 105 thermonuclease-positive (tnase-positive) cheese samples comprising 13 types, 92 (87.6%) contained coagulase-positive staphylococci, whereas 9 (8.6%) contained microorganisms other than staphylococci as the major contaminants. of the latter group, six samples contained bacillus spp. comprising three species (b. cereus, b. licheniformis, and b. subtilis), and three contained mainly enterococci (streptococcus faecalis), which were proven to be tnase producers. the organisms responsible for t ... | 1980 | 6772291 |
| effect of n-acyl substitution at glucosamine residues on lysozyme-catalyzed hydrolysis of cell-wall peptidoglycan and its oligosaccharides. | 1980 | 6772447 | |
| heat stability of bacillus cereus enzymes within spores and in extracts. | inactivation rates for nine enzymes extracted from bacillus cereus spores were measured at several temperatures, and the temperature at which each enzyme had a half-life of 10 min (inactivation temperature) was determined. inactivation temperatures ranged from 47 degrees c for glucose 6-phosphate dehydrogenase to 70 degrees c for leucine dehydrogenase, showing that spore enzymes were not unusually heat stable. enzymes extracted from vegetative cells of b. cereus had heat stabilities similar to t ... | 1980 | 6772628 |
| distribution of calcium and other elements in cryosectioned bacillus cereus t spores, determined by high-resolution scanning electron probe x-ray microanalysis. | the distribution of a number of key elements in bacillus cereus t spores was determined by high-resolution scanning electron probe x-ray microanalysis. to circumvent the redistribution of soluble or weakly bound elements, freeze-dried cryosections of spores, which had been rapidly frozen in 50% aqueous polyvinyl pyrrolidone, were employed. the sections were examined by using a modified philips em400 electron microscope fitted with a field emission gun, scanning transmission electron microscopy a ... | 1980 | 6772633 |
| lysis of erythrocytes from stored human blood by phospholipase c (bacillus cereus). | the ability of phospholipase c (bacillus cereus) to lyse erythrocytes from human blood that had been stored under transfusion service conditions for up to 16 weeks has been examined. when incubated at 20 degrees c with enzyme (0.03 mg/ml, 55 units/ml) for up to 1 h fresh erythrocytes were not lysed. after about 4 weeks of storage a population of very readily lysed erythrocytes appeared. the morphological changes in erythrocytes from blood stored up to 16 weeks were examined by scanning electron ... | 1980 | 6773524 |
| inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopencillanic acid: mechanism. | the mechanism of the inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid, a probable suicide substrate [see loosemore, m.j., cohen, s.a., & pratt, r.f. (1980) biochemistry (preceding paper in this issue)], is described. inactivation is accompanied by covalent modification of the protein with the appearance of a characteristic chromophore at 326 nm. ultraviolet (uv) absorption, nuclear magnetic resonance (nmr), and circular dichroic (cd) spectra of the modified prote ... | 1980 | 6773559 |
| mechanism of substrate-induced inactivation of beta-lactamase i. | beta-lactamase i (from bacillus cereus 569/h) is inactivated by certain substrates (e.g. methicillin or cloxacillin) but not by others (e.g. benzylpenicillin). emzyme that had been inactivated was found to be labelled stoichiometrically, as shown by the use of radioactive methicillin. use of the penamaldate reaction showed the presence of a penicilloyl group in the enzyme inactivated by either methicillin or cloxacillin. in conditions under which enzymic activity was regained the penicilloyl gro ... | 1980 | 6773776 |
| growth of bacillus cereus in media containing plant seed materials and ingredients used in chinese cookery. | 1980 | 6773916 | |
| effect of high concentrations of carbon dioxide on growth rate of pseudomonas fragi, bacillus cereus and streptococcus cremoris. | 1980 | 6773917 | |
| [preliminary study of bacterial resistance to antibiotics isolated from food products]. | 1980 | 6774403 | |
| enzymatic deacetylation of n-acetylglucosamine residues in cell wall peptidoglycan. | an enzyme which catalyzes the hydrolysis of acetamido groups of n-acetylglucosamine residues in cell wall peptidoglycan was found in the supernatant and 20,000 x g pellet fractions of bacillus cereus. autolysis of the latter fraction resulted in solubilization and activation of the deacetylase. among various bacteria, strains of b. cereus which contain high proportions of n-unsubstituted glucosamine residues in their cell wall peptidoglycan components are particularly rich in the deacetylase. th ... | 1980 | 6774970 |
| bacterial debridement of the burn eschar: the in vivo activity of selected organisms. | 1980 | 6775153 | |
| structure of linkage region between glycerol teichoic acid and peptidoglycan in bacillus cereus ahu 1030 cell walls. | 1980 | 6775628 | |
| enumeration and confirmation of bacillus cereus in foods: collaborative study. | a collaborative study was conducted in 15 laboratories to evaluate 2 different techniques for enumerating bacillus cereus in foods. a direct plating technique using mannitol-egg yolk-poly-myxin agar and a most probable number (mpn) technique using trypticase-soy-polymyxin broth were compared for the enumeration of high and low populations of b. cereus in mashed potatoes. the collaborative results showed that the overall mean recovery obtained with the low population level was essentially the sam ... | 1980 | 6776089 |
| endophthalmitis due to bacillus cereus: case report. | an acute case of endophthalmitis following ocular trauma involving barbary branches is presented. bacillus cereus var. mycoides was cultured from lens material obtained via a limbal incision. | 1980 | 6776450 |
| [comparison of culture media for quantitative determination of bacillus cereus in various food products]. | 1980 | 6776617 |