Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| mice are protected against bordetella pertussis infection by intra-nasal immunization with filamentous haemagglutinin. | intra-nasal immunization of mice with purified bordetella pertussis filamentous haemagglutinin (fha) or a crude cell sonicate was shown to protect against subsequent b. pertussis aerosol challenge. immunization with fha was found to be the most effective and resulted in complete clearance of the bacterial infection from the lungs within 14 days. serum igg and lung iga anti-fha antibodies were detectable within 4 weeks of the first immunization and anamnestic responses were seen following seconda ... | 1993 | 8472903 |
| locus controlling bordetella pertussis-induced histamine sensitization (bphs), an autoimmune disease-susceptibility gene, maps distal to t-cell receptor beta-chain gene on mouse chromosome 6. | pertussis toxin (ptx) is the primary component responsible for eliciting the majority of biological activities associated with bordetella pertussis, including the induction of several tissue-adjuvant models of organ-specific autoimmune disease. ptx, when administered in vivo, enhances vascular permeability, which is made manifest by a concomitant increase in sensitivity to a variety of agents and treatments affecting the vascular bed. one such agent is histamine, and the response to ptx, as meas ... | 1993 | 8475118 |
| biochemical and immunological properties of two forms of pertactin, the 69,000-molecular-weight outer membrane protein of bordetella pertussis. | two apparent isoforms of the virulence-associated 69,000-molecular-weight protein pertactin were purified from bordetella pertussis. mass spectrometry showed a difference of 2,060 da, which may result from differential c-terminal cleavage of a larger precursor. both forms were protective in a mouse model, eliciting bactericidal antibodies and reducing respiratory tract colonization. | 1993 | 8478113 |
| unrecognized pertussis infection in adolescents. | little information is available regarding the level of immunity to bordetella pertussis among adolescents. we measured serum antibodies in 156 healthy adolescents to the following pertussis antigens: pertussis toxin, filamentous hemagglutinin, and 69-kd outer membrane protein. in an attempt to identify intercurrent pertussis infections, we also obtained a total of 43 repeated samples during the following 5 years. using a 50% or greater rise in igg enzyme-linked immunosorbent assay titers to defi ... | 1993 | 8488807 |
| vip-induced cross-talk between g-proteins in membranes from rat anterior pituitary cells. | in order to study the activation mechanism of heterotrimeric g-proteins by agonist-liganded receptors, gtp gamma s binding to membranes was measured in rat adenohypophyseal cells after addition of dopamine (da) or vasoactive intestinal peptide (vip), which, respectively, inhibit and activate pituitary adenylyl cyclase. g-protein subunit present in anterior pituitary cells was characterized by either adp-ribosylation catalysed by bordetella pertussis and cholera toxins or by immunoblot using spec ... | 1993 | 8499223 |
| antibodies to bordetella pertussis adenylate cyclase toxin in neonatal and maternal sera. | to investigate the high prevalence among infants of antibodies to bordetella pertussis adenylate cyclase toxin (act), cord-blood sera were examined for antibodies to act, filamentous hemagglutinin (fha) and pertussis toxin (pt) using immunoblot analysis. antibodies reactive with act were the most prevalent in neonatal sera. similar reactivity of igg with act was found in each sample of a given neonatal-maternal pair, yet igm reactive with act was virtually absent in neonatal sera, suggesting tha ... | 1993 | 8499896 |
| characterization of pertussis toxin analogs containing mutations in b-oligomer subunits. | the s2, s3, and s4 subunit genes of pertussis toxin (pt) from bordetella pertussis were subjected to site-directed mutagenesis, and the resultant pt analogs were assayed for altered biological properties. pt analogs s2(t91,r92,n93) delta and s2(y102a,y103a) exhibited reduced binding to fetuin. several pt analogs with mutations in the s2, s3, or s4 subunit showed reduced in vitro toxicity, as measured in the chinese hamster ovary (cho) cell clustering assay. in particular, pt analogs s3(y82a) and ... | 1993 | 8500874 |
| flagellin gene transcription in bordetella bronchiseptica is regulated by the bvgas virulence control system. | the products of the bvgas locus activate expression of a majority of the known bordetella virulence factors but also exert negative control over a class of genes called vrg genes (bvg-repressed genes). bvgas negatively controls the production of flagella and the phenotype of motility in bordetella bronchiseptica. in this study flaa, the flagellin gene, was cloned and characterized to facilitate studies of this negative control pathway. an internal flaa probe detected hybridizing sequences on gen ... | 1993 | 8501051 |
| stimulation of secretory antibodies against bordetella pertussis antigens in the lungs of mice after oral or intranasal administration of liposome-incorporated cell-surface antigens. | lipopolysaccharide (lps) and outer membrane protein (omp) preparations of bordetella pertussis were incorporated into multilamellar liposomes composed of soya bean-derived phospholipids which were then used for oral and intranasal immunization of mice. specific antibody responses of animals immunized by either route were measured in lung washes. a specific iga response to lps was detected after immunization with the omp-containing liposomes but not with the lps-containing liposomes, indicating a ... | 1993 | 8502163 |
| composition of the peptidoglycan of haemophilus influenzae. | the composition of the peptidoglycan of haemophilus influenzae was determined by analyzing glycopeptides generated by m1 muramidase hydrolysis using high pressure liquid chromatography, fast atom bombardment mass spectrometry, and fast atom bombardment collisionally activated dissociation tandem mass spectrometry, and amino acid analysis. the structures of 17 glycopeptides, representing 96% of the total peptidoglycan, were ascertained. fifteen glycopeptides resembled species described for escher ... | 1993 | 8505290 |
| identification of a carbohydrate recognition domain in filamentous hemagglutinin from bordetella pertussis. | the adherence of bordetella pertussis to ciliated cells and macrophages is critical to colonization and infection of the respiratory tract. adherence to both types of cells involves the recognition of eukaryotic carbohydrates by the bacterial adhesin filamentous hemagglutinin (fha). the carbohydrate recognition domain (crd) of fha is considered an important antigen for subcomponent vaccines to maximize the generation of antiadherence antibodies capable of protecting against colonization. for ide ... | 1993 | 8514379 |
| enhancement of interleukin-4 production by pertussis toxin. | pertussis toxin (pt), a protein toxin of bordetella pertussis, has many biological activities, including potent adjuvant capacity and the ability to up-regulate immunoglobulin e (ige) production. interleukin-4 (il-4) is a cytokine which is essential for the ige response. accordingly, we examined the effect of pt on il-4 production. spleen and lymph node cell suspensions were prepared from immunized mice and cultured with antigen or polyclonal stimuli in vitro. il-4 secretion was assessed by bioa ... | 1993 | 8514386 |
| acellular pertussis vaccines--a solution to the pertussis problem? | the expanded knowledge of the components of bordetella pertussis has led to the production of new vaccines consisting of one or more components of the organism. the vaccines have been found to be safe and immunogenic in infants and children. early efficacy studies failed to determine what component of the vaccine and what level of antibody were needed for protection; additional studies with many more vaccines are currently in progress. the role of adults as reservoirs of b. pertussis infections ... | 1993 | 8515102 |
| acellular pertussis vaccines--a solution to the pertussis problem. | available data relating to pertussis and pertussis immunization are frequently overlooked or misinterpreted. mortality due to pertussis is underreported. most whole cell pertussis vaccines are effective; there is no evidence that pertussis vaccines cause brain damage. moreover, acellular pertussis vaccines have been used successfully in japan since 1981. third-generation, genetically derived acellular pertussis vaccines have been developed, but knowledge of antigens and concentrations required h ... | 1993 | 8515111 |
| mutations which result in constitutive expression of the bordetella pertussis filamentous haemagglutinin gene. | the expression of the filamentous haemagglutinin (fhab) of bordetella pertussis is positively regulated by the bvg locus which encodes a transcriptional activator, bvga, and a transmembrane sensor protein, bvgs. the gene encoding fhab, fhab alone, is not expressed in escherichia coli, but the introduction of the bvg locus in trans can restore fhab expression. using fhab::laczy fusions, we have isolated, in e. coli, partially bvg-independent constitutive mutants. the corresponding mutations have ... | 1995 | 8525053 |
| frequency of unrecognized bordetella pertussis infections in adults. | to investigate the frequency of unrecognized bordetella pertussis infections in adults, we performed iga and igg elisa antibody studies with four b. pertussis antigens--i.e., lymphocytosis-promoting factor, filamentous hemagglutinin, pertactin, and fimbriae-2--in 51 health care workers from whom six consecutive yearly serum samples (from 1984 to 1989) were available. overall, 90% of the subjects had a significant increase in antibody (iga or igg) to one or more antigens between 2 consecutive yea ... | 1995 | 8527557 |
| repeated sequences isolated from bordetella pertussis induce dna rearrangements and deletions at high frequency. | two repeated sequences (rs) from bordetella pertussis were cloned in escherichia coli and sequenced. the rs, called rsbp1 and rsbp3, are highly homologous to other b. pertussis rs. the recombinant plasmids containing rsbp1 and rsbp3 or transposon-like structures of these elements were not stable but segregated plasmids with deletions or rearranged dna. rs of b. pertussis seem to be able to stimulate both intra- and inter-genomic reca-independent recombination events. in at least one case, the ob ... | 1995 | 8529873 |
| efficacy of acellular pertussis vaccine in early childhood after household exposure. | to evaluate the efficacy of a three-dose primary vaccination with a diphtheria-tetanus tricomponent acellular pertussis vaccine against "typical" pertussis, defined as a spasmodic cough of 21 days or longer with confirmation of bordetella pertussis infection by culture or serology. | 1996 | 8531284 |
| immunogenicity and reactogenicity of the component acellular pertussis vaccine produced by a combination of column purified pertussis toxin and filamentous haemagglutinin. | this is the report on a prospective, single blind, comparative study of a component acellular pertussis vaccine produced by a combination of detoxified, column purified pertussis toxin (pt) and filamentous hemagglutinin (fha) combined with diphtheria and tetanus toxoids (dtcap) and the traditional acellular pertussis vaccine produced with essentially the same method as described by sato with dt (dtap) of the same manufacturer. a total of 616 infants and children received dtcap and a total of 289 ... | 1995 | 8533580 |
| comparison of media for agar dilution susceptibility testing of bordetella pertussis and bordetella parapertussis. | antimicrobial susceptibility testing of the fastidious species bordetella pertussis and bordetella parapertussis is not standardized. in an attempt to find the optimal medium for agar dilution testing, the activity of erythromycin against bordetella pertussis and bordetella parapertussis (34 isolates each) was assessed using homologous broth/agar combinations of bordet-gengou, charcoal, iso-sensitest (oxoid) and mueller-hinton media. each medium was supplemented with 5% and 20% whole defibrinate ... | 1995 | 8536725 |
| response and decline of serum igg antibodies to pertussis toxin, filamentous hemagglutinin and pertactin in children with pertussis. | the serum igg antibody response and decrease to 3 bordetella pertussis antigens was compared in children with pertussis. sera were obtained at the first clinical visit and 1, 3 and 12 months later from 89 children with > or = 3 weeks of paroxysmal cough. igg antibodies to pertussis toxin (pt), to filamentous hemagglutinin (fha) and to pertactin were determined with elisa. of 54 children with culture-confirmed pertussis or culture-confirmed familial exposure, 45 (83%) had a significant (> or = 3 ... | 1995 | 8539553 |
| [role of macrolides in the treatment of respiratory tract infections in children]. | macrolides are antibiotics with high intracellular concentrations. they have a bacteriostatic activity but are also bactericides for concentrations five times greater than the minimal inhibitory concentration, concentrations in which they reach in the respiratory tract. they are usually active on streptococcus, neisseria, moraxella catarrhalis, listeria monocytogenes, bordetella pertussis, pasteurella multocida, chlamydia, mycoplasma pneumoniae, legionella pneumophila and helicobacter pylori. th ... | 1995 | 8548000 |
| orally administered microencapsulated bordetella pertussis fimbriae protect mice from b. pertussis respiratory infection. | fimbriae from bordetella pertussis have been encapsulated in poly(lactide-co-glycolide) microparticles of a size appropriate for uptake by the immune inductive tissues of the gastrointestinal tract. mice were immunized by oral gavage with a single dose of 10 micrograms of microencapsulated fimbriae. the resulting immune responses were compared with those resulting from intraperitoneal injection of mice with equivalent amounts of fimbriae absorbed onto alhydrogel. the examination of serum and muc ... | 1996 | 8550197 |
| the ornithine decarboxylase gene odc is required for alcaligin siderophore biosynthesis in bordetella spp.: putrescine is a precursor of alcaligin. | chromosomal insertions defining bordetella bronchiseptica siderophore phenotypic complementation group iii mutants brm3 and brm5 were found to reside approximately 200 to 300 bp apart by restriction mapping of cloned genomic regions associated with the insertion markers. dna hybridization analysis using b. bronchiseptica genomic dna sequences flanking the cloned brm3 insertion marker identified homologous bordetella pertussis ut25 cosmids that complemented the siderophore biosynthesis defect of ... | 1996 | 8550442 |
| construction and characterization of bordetella pertussis reca- mutants. | antibiotic drug-resistance cassettes (drcs) were used to insertionally inactivate the wild-type bordetella pertussis reca gene cloned into a suicide vector. the mutant allele was mobilized by conjugal gene transfer from escherichia coli strain sm10 into different genetic backgrounds of b. pertussis. southern hybridization studies of one of these mutants showed that it contained a drc integrated within a reca gene situated within a clai genomic dna fragment. selected mutants were assayed to quant ... | 1995 | 8566708 |
| containment of pertussis in the regional pediatric hospital during the greater cincinnati epidemic of 1993. | to describe methods of preventing nosocomial pertussis in patients, employees, and visitors to a hospital during a communitywide epidemic in greater cincinnati. | 1995 | 8568199 |
| maintenance of bovine oocytes in prophase of meiosis i by high [camp]i. | the effects of high intracellular camp concentrations ([camp]i) on germinal vesicle maintenance of bovine cumulus-oocyte complexes were investigated, using 8-bromo-3',5'-camp (8-br-camp) or an invasive adenylate cyclase from bordetella pertussis to increase the [camp]i. the effects of interactions of these agents with macromolecular supplements in culture medium (fetal calf serum, fcs; polyvinylpyrrolidone, pvp; bsa), and different methods of processing complexes before culture, on subsequent ge ... | 1995 | 8568765 |
| a bordetella pertussis fepa homologue required for utilization of exogenous ferric enterobactin. | the bfea (bordetella ferric enterobactin) receptor gene was cloned from a bordetella pertussis chromosomal library by using a screen in escherichia coli to detect iron-repressed genes encoding exported proteins translationally fused to the e. coli phoa gene. the bfea gene encoded a protein with a molecular mass of approximately 80 kda and about 50% amino acid sequence identity to both the fepa- and pfea-encoded enterobactin receptors of e. coli and pseudomonas aeruginosa, respectively. enterobac ... | 1995 | 8574411 |
| distinct roles of the n-terminal and c-terminal precursor domains in the biogenesis of the bordetella pertussis filamentous hemagglutinin. | the 220-kda bordetella pertussis filamentous hemagglutinin (fha) is the major exported protein found in culture supernatants. the structural gene of fha has a coding potential for a 367-kda protein, and the mature form constitutes the n-terminal 60% of the 367-kda precursor. the c-terminal domain of the precursor was found to be important for the high-level secretion of full-length fha but not of truncated analogs (80 kda or less). the secretion of full-length and truncated fha polypeptides requ ... | 1996 | 8576038 |
| evaluation and validation of a monoclonal immunofluorescent reagent for direct detection of bordetella pertussis. | an outbreak of pertussis in manitoba, canada, provided an opportunity to evaluate the recently developed monoclonal antibody (mab) bl-5 for the direct detection of bordetella pertussis. the mab recognizes a lipooligosaccharide epitope. a total of 1,507 consecutive nasopharyngeal swabs for culture and companion smears for direct fluorescent-antibody (dfa) detection were evaluated at cadham provincial laboratory between september and november 1994. the cutoff for dfa positivity was four fluorescin ... | 1995 | 8576336 |
| clinical course of pertussis in immunized children. | to describe the clinical course of bordetella pertussis infection in a highly immunized childhood population, we studied prospectively endemic and epidemic pertussis in a metropolitan population with an immunization rate > 90% during an 8-year period from 1987 through 1994. patients with a possible diagnosis of pertussis were referred by family or emergency room physicians for nasopharyngeal culture. patients with a culture positive for b. pertussis were contacted by a nurse who completed a deta ... | 1995 | 8584314 |
| analysis of bordetella pertussis isolates from an epidemic by pulsed-field gel electrophoresis. | we examined genetic variation among 78 clinical isolates of bordetella pertussis, including 54 strains recovered during a 1986 pertussis epidemic. a total of 16 pulsed-field gel electrophoresis (pfge) profiles, generated with each of three different enzymes (xbai, spei, and drai), were obtained from the epidemic and sporadic isolates included in the study. indistinguishable profiles were seen among strains unrelated temporally or geographically, as well as among strains isolated sporadically fro ... | 1995 | 8586677 |
| the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species. | the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ... | 1995 | 8587109 |
| household contact study of bordetella pertussis infections. | household contacts of primary pertussis cases were evaluated. infection was determined by culture, direct fluorescent antibody assay, and serological criteria. agglutinin titers and values of elisa igg and iga antibodies to lymphocytosis-promoting factor, filamentous hemagglutinin, and pertactin were determined. in 39 households 255 subjects were exposed; 114 remained well (group 1), 53 had mild illness (group 2), and 88 had pertussis (group 3). the infection rates were 46% (group 1), 43% (group ... | 1995 | 8589145 |
| experimental autoimmune dacryoadenitis. i. lacrimal gland disease in the rat. | experimental autoimmune dacryoadenitis was produced in lewis rats by immunization with a single intradermal administration of a 3m kcl extract of exorbital lacrimal gland in cfa, when enhanced by simultaneous i.v. injection of killed bordetella pertussis. no significant lacrimal lesions were observed in control animals immunized with the extracts of harderian or salivary glands. gel filtration of the 3m kcl extract on sephacryl s-300 column yielded three protein fractions. only fraction iii (mw ... | 1987 | 8591907 |
| experimental autoimmune dacryoadenitis. ii. harderian gland disease in the rat. | experimental autoimmune dacryoadenitis was induced in 100% of lewis rats by immunization with a kcl extract of harderian gland in complete freund's adjuvant (cfa), providing that the animals had received simultaneously i.v. injection of killed bordetella pertussis. no significant pathological changes in the harderian gland were observed in control animals immunized with kcl extracts of lacrimal or salivary glands. gel filtration of the kcl extract on sephacryl s-300 column yielded three protein ... | 1987 | 8591908 |
| coupling of etb endothelin receptor to mitogen-activated protein kinase stimulation and dna synthesis in primary cultures of rat astrocytes. | astrocytes have been shown to express endothelin (et) receptors functionally coupled, via different heterotrimeric g proteins, to several intracellular pathways. to assess the relative contribution of each subtype in the astrocytic responses to et-1, effects of bq123, an antagonist selective for the et receptor subtype a (eta-r), and irl1620, an agonist selective for the et receptor subtype b (etb-r), were investigated in primary cultures of rat astrocytes. binding experiments indicated that the ... | 1996 | 8592114 |
| induction of macrophage apoptosis by bordetella pertussis adenylate cyclase-hemolysin. | we previously demonstrated that bordetella pertussis induced macrophage apoptosis and that adenylate cyclase-hemolysin expression is required for this phenomenon. in order to determine a role for other b. pertussis factors, such as pertussis toxin or the adhesins, pertactin and filamentous hemagglutinin, we compared mutants deficient in adhesin expression or purified proteins for their ability to induce cell death. we showed that only purified adenylate cyclase-hemolysin, but neither adhesins, n ... | 1995 | 8593951 |
| identification by in vitro complementation of regions required for cell-invasive activity of bordetella pertussis adenylate cyclase toxin. | the adenylate cyclase toxin (cyaa) of bordetella pertussis is a 1706-residue protein composed of an amino-terminal adenylate cyclase (ac) domain linked to a 1300-residue channel-forming rtx (repeats in toxin) haemolysin. the toxin delivers its ac domain into a variety of eukaryotic cells and impairs cellular functions by catalysing unregulated synthesis of camp from intracellular atp. we have examined toxin activities of a set of deletion derivatives of cyaa. the results indicate that cyaa does ... | 1995 | 8594322 |
| domain structure of the outer membrane transporter protein cyae of bordetella pertussis. | 1995 | 8594341 | |
| phosphorylated bvga is sufficient for transcriptional activation of virulence-regulated genes in bordetella pertussis. | in bordetella pertussis the expression of virulence factors is coordinately regulated by the bvgs and bvga proteins, members of the bacterial two-component signal transduction family, bvgs being the transmembrane sensor and bvga the regulator. activation of virulence gene expression requires phosphorylation of bvga. on the basis of observed differences in the regulation of individual genes, the existence of accessory regulators has been postulated. they were supposed to be necessary for expressi ... | 1996 | 8598192 |
| integration of multiple domains in a two-component sensor protein: the bordetella pertussis bvgas phosphorelay. | bvgs and bvga, a two-component system, regulate virulence gene expression in bordetella pertussis. bvgs is a transmembrane sensor protein that can autophosphorylate and phosphorylate bvga. phosphorylated bvga activates transcription of virulence genes. the cytoplasmic region of bvgs contains three domains separated by alanine/proline-rich sequences--the transmitter, receiver and c-terminus. we report that the c-terminal domain, like the transmitter and receiver, is an essential part of the phosp ... | 1996 | 8605872 |
| nitric oxide induction by pertussis toxin in mouse spleen cells via gamma interferon. | we examined the major pathogenic substances of bordetella pertussis for the ability to induce nitric oxide, and important biological function of macrophages, via gamma interferon in spleen cells. b. pertussis, which produces a variety of pathogenic substances, including pertussis toxin and filamentous hemagglutinin, causes a severe respiratory disease. nitric oxide was detected in the culture fluid of spleen cells stimulated with pertussis toxin or its b oligomer but not in the culture fluid of ... | 1996 | 8606094 |
| the pertussis toxin liberation genes of bordetella pertussis are transcriptionally linked to the pertussis toxin operon. | the dna sequence of the pertussis toxin operon (ptx) of bordetella pertussis predicts that transcription of the operon ends downstream from the ptxs3 gene at a possible stem-loop structure. secretion of the assembled pertussis toxin into the culture medium required the expression of 8 genes arranged in an operon (ptl) and lying 55 bp downstream from the ptx and ptl operons are cotranscribed and coregulated by the p(tox) promoter. deletion of the 55-bp dna region caused an increase in the amount ... | 1996 | 8606119 |
| polymerase chain reaction for the detection of bordetella pertussis in clinical nasopharyngeal aspirates. | a pcr procedure for the detection of bordetella pertussis in nasopharyngeal aspirates (npas) was developed with primers derived from the pertussis toxin promoter region. the amplification resulted in a 191-bp pcr product specific for b. pertussis. a total of 681 npas collected from children with cough lasting >7 days was evaluated by pcr and culture; 104 aspirates were positive by pcr and 93 by culture. sixteen cases were positive only by pcr and five culture positive aspirates were negative by ... | 1996 | 8606353 |
| structure of bordetella pertussis virulence factor p.69 pertactin. | a new generation of whooping-cough vaccines contain p.69 pertactin, a surface-exposed domain of an outer membrane protein expressed by the virulent bacterium bordetella pertussis. this protein is a virulence factor that mediates adhesion to target mammalian cells, a reaction that is in part mediated by an rgd sequence. the x-ray crystal structure of p.69 pertactin has been determined to 2.5 a. the protein fold consists of a 16-stranded parallel beta-helix with a v-shaped cross-section, and is th ... | 1996 | 8609998 |
| expression of high- and low-affinity receptors for c3a on the human mast cell line, hmc-1. | the proteolytic cleavage product of complement component 3, (c3a), is like c4a and c5a, is a potent anaphylatoxin and induces the production of inflammatory mediators in phagocytes. notably, mast cells respond to c3a with the release of vasoactive substances, including histamine. we have examined the function and receptor binding of c3a in a human leukemic mast cell line, hmc-1. similar to chemoattractant agonists in leukocytes, c3a induced rapid cytosolic free calcium concentration increases in ... | 1996 | 8625964 |
| the seroepidemiology of bordetella pertussis infections: a study of persons ages 1-65 years. | although the incidence of bordetella pertussis infections had decreased significantly since the introduction of widespread vaccination, an increase in the number of cases has occurred recently. in an attempt to define the seroepidemiology of pertussis in nashville, antibody levels to pertussis toxin (pt) and filamentous hemagglutinin (fha) were measured by elisa in 585 serum samples from healthy 1- to 65-year-old subjects. data were analyzed by smooth curve fitting and by comparison of trends in ... | 1996 | 8627081 |
| synthetic peptide derived from the bordetella pertussis bacterium reduces infarct volume after transient middle cerebral artery occlusion in the rat. | we explored the therapeutic potential of a peptide (f20) derived from the filamentous hemagglutinin of bordetella pertussis in a model of ischemic cell injury after transient (2 hours) middle cerebral artery (mca) occlusion in the rat. animals were divided into two groups-(1) f20 peptide group: rats (n = 11) were subjected to 2 hours of transient mca occlusion, and f20 peptide was administered intravenously (50 nmol) at 0 hours of reperfusion and intraperitoneally (150 nmol/dose) at 2, 4, 6, 8, ... | 1996 | 8628495 |
| pertussis deaths: report of 23 cases in the united states, 1992 and 1993. | to characterize pertussis deaths and to identify possible risk factors and prevention strategies. | 1996 | 8628595 |
| dna binding of the bordetella pertussis h1 homolog alters in vitro dna flexibility. | bph1, the bordetella pertussis h1 homolog, interacts with chromosomal dna. with dnase i protection assays, we demonstrate in this study that bph1 binds dna in a nonspecific manner and that it may cover dna fragments from end to end. although the binding was shown to be nonspecific, preferential binding sites and sites resistant to bph1 binding were identified within and upstream of the pertussis toxin promoter sequence. in the presence of dna ligase, bph1 favored the formation of multimeric dna ... | 1996 | 8631692 |
| crystal structure of the pertussis toxin-atp complex: a molecular sensor. | pertussis toxin is a major virulence factor of bordetella pertussis, the causative agent of whooping cough. the protein is a hexamer containing a catalytic subunit (s1) that is tightly associated with a pentameric cell-binding component (b-oligomer). in vitro experiments have shown that atp and a number of detergents and phospholipids assist in activating the holotoxin by destabilizing the interaction between s1 and the b-oligomer. similar processes may play a role in the activation of pertussis ... | 1996 | 8637000 |
| prevalence and incidence of adult pertussis in an urban population. | to determine the prevalence of bordetella pertussis infection among adults who have prolonged cough for 2 weeks or longer and to estimate the incidence of b pertussis infection in adults in a defined urban population. | 1996 | 8637142 |
| native, but not genetically inactivated, pertussis toxin protects mice against experimental allergic encephalomyelitis. | treatment of sjl mice with 400 ng bordetella pertussis toxin (pt) either in saline or emulsified in incomplete freund's adjuvant protected the mice against experimental autoimmune encephalomyelitis (eae) induced 28 days later by a synthetic peptide of myelin proteolipid protein (plp139-151) in complete freund's adjuvant. however, treatment with a genetically inactivated pertussis toxin in which the catalytic and nad-binding sites of the adp-ribosyltransferase subunit were modified by site-direct ... | 1996 | 8640862 |
| pertussis in german adults. | in a large pertussis vaccine efficacy trial in germany, vaccinees and/or their family members were seen if a cough illness of >14 days was reported. evidence of recent bordetella pertussis infection included a positive culture and/or polymerase chain reaction (pcr) and/or significant antibody values in agglutination and/or elisa assay. from july 1991 through february 1994, 246 adults were evaluated and 64 had evidence of b. pertussis infection; of these, 38% had whooping, 26% had a history of pr ... | 1995 | 8645831 |
| conformational transitions within the calmodulin-binding site of bordetella pertussis adenylate cyclase studied by time-resolved fluorescence of trp242 and circular dichroism. | the sequence situated around trp242 in bordetella pertussis adenylate cyclase, a bifunctional protein of 1706 amino acid residues, forms the core of the calmodulin-binding site. peptides varying in size and in affinity for calmodulin, and preserving the same sequence around trp242 were analyzed by time-resolved fluorescence spectroscopy. their dynamic properties were compared to those of the catalytic domain of b. pertussis adenylate cyclase corresponding to the first 400 amino acid residues of ... | 1996 | 8647105 |
| in vivo induction of ctl responses by recombinant adenylate cyclase of bordetella pertussis carrying viral cd8+ t cell epitopes. | exogenous ags enter the endosomal pathway and are presented to cd4+ t cells in association with class ii molecules whereas endogenously synthesized ags, such as viral proteins, are presented to cd8+ t cells in association with mhc class i molecules. therefore, most ctl activation strategies use live vectors although an alternative possibility could be to deliver the epitope into the cytosol by targeting it to an invasive nonreplicative vector. the adenylate cyclase toxin of bordetella pertussis ... | 1996 | 8648115 |
| pertussis vaccines: acellular versus whole-cell. | acellular pertussis vaccines containing purified bordetella pertussis antigens have now been extensively field tested. they produce a significantly lower rate of reactions than whole-cell vaccines and their efficacy is either comparable or superior. at least three antigens appear necessary for good protection: pertussis toxoid, filamentous haemagglutinin and pertactin (an outer-membrane protein); fimbrial agglutinogens are probably not needed. it is hoped that a cellular pertussis vaccine will s ... | 1996 | 8649299 |
| identification and characterization of bph2, a novel histone h1 homolog in bordetella pertussis. | a basic protein, bph2, with an apparent molecular mass of 18 kda was purified from bordetella pertussis, and the corresponding gene, bph2, was cloned. sequence analysis revealed some homology to the h1 class of eukaryotic histones and to algp protein of pseudomonas aeruginosa. bph2 binds both single- and double-stranded dna in a nonspecific manner. deletion of the corresponding gene in b. pertussis generated a bph2 null mutant with an altered growth rate in which the expression of two virulence ... | 1996 | 8655481 |
| a clinical validation of bordetella pertussis and bordetella parapertussis polymerase chain reaction: comparison with culture and serology using samples from patients with suspected whooping cough from a highly immunized population. | the aim of this study was to validate the performance of the polymerase chain reaction (pcr) assay for bordetella pertussis and bordetella parapertussis in comparison with both culture and serology. the number of samples positive in pcr was 2.4-fold higher than the number of samples positive in culture. in serologically confirmed cases, the sensitivity of pcr and culture depended on the duration of disease and the age of the patient, being less sensitive in older age and later in disease. the se ... | 1996 | 8656019 |
| safety and effectiveness of an acellular pertussis vaccine in subjects with down's syndrome. | we evaluated the reactogenicity and immunogenicity of an acellular pertussis vaccine in 24 subjects affected by down's syndrome and in 10 normal infants. neither general nor local adverse reactions were observed in either group of subjects. the new acellular vaccine administration elicited protective levels of antibodies in all the subjects with down's syndrome, although the geometric mean titres of igg antibodies against bordetella pertussis in these subjects were significantly lower than in no ... | 1996 | 8674075 |
| detection of bordetella pertussis by polymerase chain reaction and culture in the nasopharynx of erythromycin-treated infants with pertussis. | pertussis is a highly contagious respiratory disease and the most serious effects occur in young infants. recently it has been shown that rapid and highly specific pcr can be a useful diagnostic tool for detection of pertussis infection. to our knowledge there are no previous studies concerning the disappearance of bordetella pertussis dna from the nasopharynx during antimicrobial treatment. | 1996 | 8684877 |
| interaction between bordetella pertussis vaccine and chlorpromazine in mouse experiments. | in acute toxicity experiments the mortality of mice pretreated with bordetella pertussis vaccine increased on the first day following chlorpromazine (cpz) treatment, compared to control animals treated with cpz alone. initially, the increased drug sensitivity observed after combined treatment was attributed to summation of the toxic effects. however, the cumulation of mortality did not cease on the following days, furthermore, an increase of bacterial translocation was observed on days 6 and 7, ... | 1995 | 8689092 |
| the major fimbrial subunit of bordetella pertussis binds to sulfated sugars. | bordetella pertussis fimbriae are composed of major and minor subunits, and recently it was shown that the minor fimbrial subunit binds to vla-5, a receptor located on monocytes (w. hazenbos, c. geuijen, b. van den berg, f. mooi, and r. van furth, j. infect. dis. 171:924-929, 1995). here we present evidence that the major subunits bind to sulfated sugars, which are ubiquitous in the respiratory tract. binding was observed to chondroitin sulfate, heparan sulfate, and dextran sulfate but not to de ... | 1996 | 8698492 |
| promotion of bacteriophage induction and recombination by the cloned bordetella pertussis reca gene is copy-number dependent. | favre and coworkers (favre et al., biochimie 73:235-244, 1991) previously reported that the bordetella pertussis reca gene present at high copy number could promote a low frequency of recombination, but not bacteriophage induction in escherichia coli reca- mutants. reexamination of these phenotypes demonstrated that, in contrast to the previous study, when this gene is present at high copy number, it can stimulate a 2- to 4-log frequency of bacteriophage induction in the presence of mitomycin c, ... | 1996 | 8704660 |
| mechanisms of pertussis toxin-induced myelomonocytic cell adhesion: role of mac-1(cd11b/cd18) and urokinase receptor (cd87). | stimulation of monoblastic u937 cells with transforming growth factor beta 1 and 1,25-(oh)2 vitamin d3 (tgf-beta 1/d3) upregulates urokinase receptor (upar) and confers urokinase-dependent adhesiveness to the cells for serum- or vitronectin-coated surfaces. recent studies show that upar itself is a high-affinity adhesion receptor for vitronectin and that urokinase (upa) is an activator of this adhesive function. in the course of exploring possible g-protein involvement in this adhesion it was ob ... | 1996 | 8707356 |
| poly(lactide-co-glycolide) microencapsulation of vaccine antigens. | fimbriae from bordetella pertussis have been encapsulated in poly(lactide-co-glycolide) (plg) microspheres of a size appropriate for oral administration. the binding of antibodies which react with conformational or linear fimbrial epitopes, to fimbriae released from microspheres, suggested that the process of was not detrimental to the native integrity of the protein. mice were immunised by oral gavage with a single dose of microencapsulated fimbriae, or with fimbriae adsorbed onto alhydrogel an ... | 1996 | 8717383 |
| analysis of bordetella pertussis suspensions by elisa and flow cytometry. | 1996 | 8718594 | |
| in vitro and in vivo induction of nitric oxide by murine macrophages stimulated with bordetella pertussis. | nitric oxide (no) exhibits potent antimicrobial activity in vitro. the function of no in host defenses in vivo, however, is presently unclear. experiments were undertaken to determine the production of no in vitro from murine peritoneal and alveolar macrophages, and murine macrophage cell line (j774a.1) stimulated with bordetella pertussis or pertussis toxin (pt). in addition, we determined circulating levels of no in the sera and bronchoalveolar lavage (bal) fluids of mice infected intranasally ... | 1996 | 8731016 |
| epitope mapping the fim2 and fim3 proteins of bordetella pertussis with sera from patients infected with or vaccinated against whooping cough. | antibody-binding epitopes on the fim2 and fim3 proteins of bordetella pertussis, which have been associated with the induction of protective antibody, were located using sera from 12 patients with whooping cough and 4 vaccinated children. fifteen epitopes were identified on both fim2 and fim3. in each case 9 were recognised by serum antibody from 11 or more infected patients. epitopes associated with the highest igg activity were not the same as those associated with the highest iga activity. no ... | 1996 | 8731026 |
| calcium handling and purinoceptor subtypes involved in atp-induced contraction in rat small mesenteric arteries. | 1. the relationship between the stimulation of atp receptors, the increase in intracellular free calcium concentration ([ca2+]i; measured using the fluorescent indicator fura-2), contraction and the subtypes of purinoceptors involved were investigated in the small mesenteric artery of the rat. 2. in normal physiological solution, atp (0.001-3 mm) caused concentration-dependent increases in both [ca2+]i and contraction. both responses produced by atp (1 mm) were inhibited by 50% in the presence o ... | 1996 | 8734982 |
| detection of bordetella pertussis by rapid-cycle pcr and colorimetric microwell hybridization. | the use of rapid-cycle pcr combined with colorimetric microwell hybridization for detecting bordetella pertussis was investigated. rapid cycling was performed with an air thermocycler (model 1605; idaho technology, idaho falls, idaho). although the instrument was originally designed to be used with capillary tubes, an adapter that allows this instrument to be used with pcr tubes has recently been introduced. because of the low heat capacity of air, the thermocycler has rapid transition rates bet ... | 1996 | 8735080 |
| phosphorylation-dependent binding of bvga to the upstream region of the cyaa gene of bordetella pertussis. | in bordetella pertussis, transcription of virulence-associated genes is regulated by the bvgs and bvga proteins, members of the bacterial two-component signal-transduction family. bvgs is the transmembrane sensor and bvga, in its phosphorylated form, is believed to be the key transcriptional activator in b. pertussis. however, the bvga recognition sites in most virulence promoters have not yet been identified. to investigate the interaction of bvga with the upstream region of cyaa, the gene enco ... | 1996 | 8736528 |
| g proteins in adipocytes and preadipocytes: characterization, subcellular distribution, and potential roles for gi2 and/or gi3 in the control of cell proliferation. | guanosine triphosphate (gtp)-binding protein subunits were studied by immunoblot analysis in particulate fractions from mature adipocytes, confluent preadipocytes, and in vitro-differentiated preadipocytes. mature adipocytes express gi alpha 1, gi alpha 2, gi alpha 3, go alpha, gq/11 alpha, g13 alpha and the long and short isoforms of gs alpha, but no gz alpha or g12 alpha. confluent and differentiated preadipocytes differ in having a higher content of gi alpha 3 and g13 alpha and expressing g12 ... | 1996 | 8736707 |
| effects of dibutyryl camp and bacterial toxins on indoleamine-induced encystment of dinoflagellates. | dinoflagellates are the causative agents of red tides with worldwide occurrence and can be induced to encyst by in doleamines such as melatonin and 5-methoxytryptamine (5-mot). this biological response may be mediated via indoleamine-binding proteins or receptors. here we report the initial characterization of the signal transduction mechanisms by which indoleamines induce encystment of dinoflagellates. in particular, we explored the possible involvement of g proteins and camp in cyst formation. ... | 1996 | 8739320 |
| respiratory failure caused by dual infection with bordetella pertussis and respiratory syncytial virus. | two infants with pneumonia caused by both bordetella pertussis and respiratory syncytial virus (rs virus) suffered respiratory failure preceded by convulsion. detection of respiratory pathogens with polymerase chain reaction and enzyme-linked immunosorbent assay was crucial in the management of dually infected infants. | 1996 | 8741324 |
| pertussis toxin enhanced igg1 and ige responses to primary tetanus immunization are mediated by interleukin-4 and persist during secondary responses to tetanus alone. | pertussis toxin (ptx), the major toxin product of bordetella pertussis, has potent immunologic effect including adjuvant effects on antibody responses and sensitization for anaphylaxis. in order to further define the effect of ptx on the class and subclass of murine antibody response, we measured total and antigen specific igg subclasses and ige in balb/c mice after primary and secondary immunization with tetanus toxoid (tt). low doses of ptx (100 ng) given intravenously at the time of primary i ... | 1996 | 8744555 |
| purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by bordetella pertussis and bordetella bronchiseptica. | hydroxamate siderophores of virulent bordetella pertussis and bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(s),11,18(s)- tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone+ ++, which was previously isolated from the taxonomically-related bacterial species alcaligenes denitrificans subsp. xylosoxydans. alcal ... | 1996 | 8744901 |
| induction of mucosal immune responses against a heterologous antigen fused to filamentous hemagglutinin after intranasal immunization with recombinant bordetella pertussis. | live vaccine vectors are usually very effective and generally elicit immune responses of higher magnitude and longer duration than nonliving vectors. consequently, much attention has been turned to the engineering of oral pathogens for the delivery of foreign antigens to the gut-associated lymphoid tissues. however, no bacterial vector has yet been designed to specifically take advantage of the nasal route of mucosal vaccination. herein we describe a genetic system for the expression of heterolo ... | 1996 | 8755582 |
| a new gene locus of bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins. | recently, a novel type of regulatory mutation causing differential effects on the expression of virulence genes due to a slight overexpression of the rna polymerase alpha subunit (rpoa) was found in bordetella pertussis (n. h. carbonetti, t. m. fuchs, a. a. patamawenu, t. j. irish, h. deppisch, and r. gross, j. bacteriol. 176:7267-7273, 1994). to gather information on the molecular events behind this phenomenon, we isolated suppressor mutants of the rpoa-overexpressing strains after random mutag ... | 1996 | 8755871 |
| identification and characterization of iron-regulated bordetella pertussis alcaligin siderophore biosynthesis genes. | bordetella bronchiseptica mutants brm1, brm6, and brm9 fail to produce the native dihydroxamate siderophore alcaligin. a 4.5-kb bamhi-smal bordetella pertussis genomic dna fragment carried multiple genes required to restore alcaligin production to these siderophore-deficient mutants. phenotypic complementation analysis using subclones of the 4.5-kb genomic region demonstrated that the closely linked brm1 and brm9 mutations were genetically separable from the brm6 mutation, and both insertions ex ... | 1996 | 8759851 |
| expression and secretion of beta-glucuronidase and pertussis toxin s1 by streptomyces lividans. | streptomyces lividans iaf18, obtained by homologous cloning, is capable of over-producing xlna. to investigate the possibility of the expression of foreign genes, various coding regions of the xylanase a gene (xlna) were analysed. expression/secretion vectors were constructed containing the regulatory elements of xlna with the coding region of the leader peptide with or without the truncated structural gene encoding the first 310 amino acids of the xlna. the genes coding for the escherichia coli ... | 1996 | 8766700 |
| signal transduction and virulence regulation in bordetella pertussis. | bordetella pertussis, the causative agent of whooping cough, coordinately regulates the expression of its virulence factors in response to certain environmental stimuli. this coordinate regulation is accomplished by the bvg locus encoding the bvgs and the bvga proteins, which are members of the two-component family of bacterial signal transducing proteins. the sensor protein bvgs shows an "unorthodox" domain structure, combining the characteristic communication modules both of the two component ... | 1996 | 8767703 |
| acellular pertussis vaccine composed of genetically inactivated pertussis toxin. | whooping cough, an acute respiratory disease affecting over sixty million infants, can be prevented by vaccination. the vaccine currently used, composed of killed bacterial cells, however, has been associated with many side effects. an improved vaccine against the disease should contain pertussis toxin (pt), a major virulent factor of bordetella pertussis (b. pertussis). in order to be included in the vaccine, pt needs to be detoxified and the chemical methods used so far are not completely sati ... | 1995 | 8768791 |
| long-term human serum antibody responses after immunization with whole-cell pertussis vaccine in france. | three hundred sixty children were tested for pertussis serology 0.5 to 1.58 months after complete whole-cell pertussis vaccination. an immunoblot assay was used to detect serum antibodies to pertussis toxin, filamentous hemagglutinin, adenylate cyclase-hemolysin, and pertactin, and agglutination was used for detection of anti-agglutinogen antibodies. antibodies against pertussis toxin, pertactin, and agglutinogens decreased rapidly after vaccination but increased secondarily, suggesting exposure ... | 1996 | 8770511 |
| th1/th2 cell dichotomy in acquired immunity to bordetella pertussis: variables in the in vivo priming and in vitro cytokine detection techniques affect the classification of t-cell subsets as th1, th2 or th0. | in studies of the mechanism of immunity to bordetella pertussis in a murine respiratory infection model, we have previously demonstrated that natural infection of immunization with a whole cell vaccine induces a potent protective immune response, which is mediated by t-helper type-1 (th1) cells. in contrast an acellular vaccine generates th2 cells and is associated with delayed bacterial clearance following respiratory challenge. in the present study we have investigated the apparent th1/th2 cel ... | 1996 | 8778021 |
| the differentiation of bordetella parapertussis and bordetella bronchiseptica from humans and animals as determined by dna polymorphism mediated by two different insertion sequence elements suggests their phylogenetic relationship. | we describe a novel insertion sequence (is) element, is1002, which was found to be closely related to is481, which is found only in bordetella pertussis; we found that these two is elements have a level of sequence identity of 61.5% and also have almost identical terminal inverted repeats. is1002 was present in both b. pertussis and bordetella parapertussis strains isolated from humans. in contrast, is1002 was absent from b. parapertussis strains isolated from sheep. a dna fingerprint analysis p ... | 1996 | 8782670 |
| no cross-reactivity with filamentous hemagglutinin of bordetella pertussis in sera from patients with nontypable haemophilus influenzae pneumonia. | 1996 | 8783367 | |
| acute bordetella pertussis infection in an adult. | bordetella pertussis was transmitted from an immunized boy to his father and possibly to other family members. this case report demonstrates that although unusual, acute adult b. pertussis infection can occur. b. pertussis immunization may not prevent infection but can reduce the severity. b. pertussis was detected in sputum from the adult by direct-fluorescent antibody staining and was grown on regan-lowe medium. serology tests confirmed the infection in the adult. | 1996 | 8789030 |
| acellular pertussis vaccines: a turning point in infant and adolescent vaccination. | whooping cough, an infectious disease caused by the gram-negative bacterium bordetella pertussis, is a life-threatening disease that cannot be controlled by antibiotic treatment or other procedures of modern medicine. immunization, using a vaccine made of heat-killed bacteria, has been the only way to prevent the disease and keep the infection under control. however, the high reactogenicity of the whole-cell vaccine available so far has made vaccination very controversial, and vaccine use has be ... | 1996 | 8789596 |
| respiratory infections: community-acquired pneumonia and newer microbes. | respiratory infections, especially community-acquired forms of pneumonia (cap), are challenging for clinicians because (1) a causative microorganism can only be found in about 50% of cases; (2) initial therapy, therefore, must be based on a probable or most likely etiology in the context of the patient's overall medical condition; and (3) new microbes or those considered previously as normal flora or less virulent forms seem responsible for some cases. it is important to be acquainted with new c ... | 1996 | 8791258 |
| purification and partial characterization of a novel antibacterial agent (bac1829) produced by staphylococcus aureus ksi1829. | a novel antimicrobial agent from staphylococcus aureus ksi1829, designated bac1829, was purified by sequential steps of ammonium sulfate precipitation, sephadex g-50 gel filtration chromatography, and hydrophobic interaction chromatography. purified bac1829 has a molecular mass of 6,418 +/- 2 da. the peptide in heat stable, since full biological activity is retained after heating at 95 degrees c for 15 min, and it is destroyed by digestion with proteases. amino acid sequence analysis revealed a ... | 1996 | 8795206 |
| age-associated changes in lymphoid and antigen-presenting cell functions in mice immunized with trypanosoma cruzi antigens. | the purpose of these studies was to analyze the role of different immune cell populations in the immune response against trypanosoma cruzi antigens in aged mice. mice of different ages (3 and 12 months old) were immunized i.d. with s-105 plus bordetella pertussis as adjuvant and we compared the activities of the lymph node cells taken from 3- and 12-month-old donor animals to transfer dth or antibody production to 3-month-old recipients. this study revealed that adherent and non-adherent immune ... | 1996 | 8803920 |
| conserved sequence motifs in the unorthodox bvgs two-component sensor protein of bordetella pertussis. | the unorthodox two-component sensor protein bvgs of bordetella pertussis contains several interesting sequence motifs of unknown functional relevance, such as a histidine motif in its output domain, which is conserved among several unorthodox sensor proteins, a putative nucleotide binding site [walker box type a] in its linker region, and a region in its periplasmic domain with significant homology to the tonb protein of escherichia coli. we investigated potential functions of these sequences by ... | 1996 | 8804390 |
| genetic susceptibility to experimental autoimmune uveoretinitis in the rat is associated with an elevated th1 response. | this study examines whether genetic susceptibility vs genetic resistance to experimental autoimmune uveoretinitis (eau) are connected to a predisposition to mount a th1-dominated (ifn-gamma high, il-4 low) vs a th2-dominated (il-4 high, lfn-gamma low) response. lewis rats developed disease with high incidence after immunization with the uveitogenic peptide r16, whereas f344 rats were resistant. primed lymph node cells from both strains proliferated in culture in response to r16. however, while t ... | 1996 | 8805672 |
| in vivo and in vitro analysis of bordetella pertussis catalase and fe-superoxide dismutase mutants. | bordetella pertussis produces a catalase and a fe-superoxide dismutase. the importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. the catalase-deficient mutant survived within polymorphonuclear leukocytes, killed j774a.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. these results suggest no direct role for catalase in b. pertussis virulence. the absence o ... | 1996 | 8810507 |
| migratory response of human nk cells to monocyte-chemotactic proteins | nk cells are present mostly in blood and spleen but under certain pathological and physiological conditions rapidly accumulate at extrahematic sites. the present study investigates the responsiveness of nk cells to c-c chemokines and the mechanisms of emigration from the bloodstream. mcp-1 induced migration across polycarbonate filters of il-2-activated nk cells, whereas it was a weak attractant for unstimulated cells. the related chemokines mcp-2 and mcp-3 were also active. il-2-activated nk ce ... | 1996 | 8812655 |
| three-dimensional structure of bordetella pertussis fimbriae. | we describe the helical structure of bordetella pertussis fimbriae of serotype 3/6 as determined to a resolution of approximately 2.5 nm by three-dimensional reconstruction of negatively stained electron micrographs. the fimbria has a distinctly polar structure whose axial repeat of 13 nm contains five copies of the fim3 gene product (22 kda) in two complete turns. these subunits are connected by interactions along the fimbrial backbone which, unlike other classes of bacterial fimbriae, has no a ... | 1996 | 8812982 |
| immunomagnetic separation and solid-phase detection of bordetella pertussis. | in the present study, novel solid-phase methods were used for both sample preparation and pcr detection of bordetella pertussis. the sample preparation was performed by immunomagnetic separation with paramagnetic beads coated with polyclonal antibodies directed toward the surface antigens of the bacteria. the precoated immunobeads were directly used on nasopharyngeal aspirates to capture the bacteria on the solid support and were subsequently transferred to the pcr tube with no further manipulat ... | 1996 | 8815083 |
| validation of nested bordetella pcr in pertussis vaccine trial. | a nested pcr, using a 239-bp sequence in the pertussis toxin promoter region, was developed and evaluated. the assay differentiates bordetella pertussis, bordetella parapertussis, and bordetella bronchiseptica by restriction enzyme analysis of the amplified fragments. the diagnostic performance of the pcr was evaluated in a swedish pertussis vaccine efficacy trial which took place from 1992 to 1995, including study children and household members and using culture and serology for laboratory conf ... | 1996 | 8815088 |