Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes. | cortical endoplasmic reticulum (cer) is a permanent feature of yeast cells but occurs transiently in most animal cell types. ist2p is a transmembrane protein that permanently localizes to the cer in yeast. when ist2 is expressed in mammalian cells, it induces abundant cer containing ist2. ist2 cytoplasmic c-terminal peptide is necessary and sufficient to induce cer. this peptide sequence resembles classic coat protein complex i (copi) coatomer protein-binding kkxx signals, and indeed the dimeriz ... | 2010 | 20351264 |
| clp36 interacts with palladin in dorsal root ganglion neurons. | clp36, a member of the alpha-actinin-associated lim protein (alp)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. however, the underlying molecular mechanisms are not known. in this study, we performed yeast two-hybrid screening of an e18 mouse whole-body cdna library with clp36 as the bait and isolated palladin as a clp36-binding protein. palladin is an actin-binding protein and it was shown to have a role in the extension of cortical neurons. a coimmu ... | 2010 | 20381583 |
| role of potassium channels in stretch-promoted atrial natriuretic factor secretion. | the cardiac polypeptide hormone atrial natriuretic factor (anf) plays important roles in the regulation of blood volume and pressure. few specific details are known about basal or stretch-promoted anf secretion. here, we investigated the involvement of k(+) channels in anf secretion based on investigations of their nature as revealed by oligonucleotide microarray analysis and on protein-protein interactions evidenced by a yeast two-hybrid approach using a heterotrimeric galphao-1 g protein subun ... | 2009 | 20409940 |
| prestin surface expression and activity are augmented by interaction with map1s, a microtubule-associated protein. | prestin is a member of the slc26 family of anion transporters that is responsible for outer hair cell (ohc) electromotility. measures of voltage-evoked charge density (q(sp)) of prestin indicated that the protein is highly expressed in ohcs, with single cells expressing up to 10 million molecules within the lateral membrane. in contrast, charge density measures in transfected cells indicated that they express, at best, only a fifth as many proteins on their surface. we sought to determine whethe ... | 2010 | 20418376 |
| synthesis, antimicrobial, and anti-inflammatory activities of novel 5-(1-adamantyl)-4-arylideneamino-3-mercapto-1,2,4-triazoles and related derivatives. | the reaction of 5-(1-adamantyl)-4-amino-3-mercapto-1,2,4-triazole (5) with various aromatic aldehydes in ethanol or acetic acid yielded the corresponding 4-arylideneamino derivatives 6a-v. treatment of the 4-(2,6-difluoro- and dichlorobenzylideneamino) derivatives 6o and 6q with 1-substituted piperazines, and formaldehyde solution in ethanol afforded good yields of the corresponding 5-(1-adamantyl)-4-(2,6-dihalobenzylideneamino-2-(4-substituted-1-piperazinylmethyl)-1,2,4-triazoline-3-thiones 7a- ... | 2010 | 20428062 |
| yeast-incorporated gallium promotes fracture healing by increasing callus bony area and improving trabecular microstructure on ovariectomized osteopenic rats. | the purpose of this study was to analyze the impact of yeast-incorporated gallium on fracture healing in ovariectomized osteopenic rats. forty wistar female rats used were divided into three groups: sham-operated rats (sham), ovariectomized (ovx) rats, and ovx rats treated with yeast-bound gallium (yg). a standardized fracture-healing model with stable plate fixation was established for rat femoral. after 4-week stable fixation, animals were killed to prepare bones for micro-ct, biomechanical te ... | 2011 | 20446054 |
| [lowered lipids efficiency of compound red yeast rice extract in rats with hyperlipidemia]. | to observe the effects of compound red yeast rice extract (cryre) on blood lipids, hemorheology and blood coagulant system in rats with hyperlipidemia. | 2010 | 20450054 |
| antipyretic, analgesic, anti-inflammatory and antioxidant activities of two major chromenes from melicope lunu-ankenda. | melicope lunu-ankenda (gaertn.) t.g. hartley is used in indian traditional medicine for fever, improving complexion and as a tonic. previous studies have isolated fungicidal, antifeedant, anti-inflammatory and immunomodulatory compounds from melicope lunu-ankenda. this study is aimed at the isolation and biological activity screening of potential molecules from the volatile oils and extracts of melicope lunu-ankenda in the light of traditional applications. | 2010 | 20457245 |
| sparc interacts with ampk and regulates glut4 expression. | amp-activated protein kinase (ampk) is a critical regulator of glucose metabolism. to elucidate the biochemical mechanisms by which ampk regulates glucose and fat metabolism, we conducted a yeast two-hybrid screen to identify its interacting partners. a yeast two-hybrid system was used to screen a mouse embryo cdna library for proteins able to bind mouse ampk alpha 1. we also demonstrated an endogenous interaction between ampk alpha 1 and its interacting partner by co-immunoprecipitation of the ... | 2010 | 20460104 |
| prediction of functionally selective allosteric interactions at an m3 muscarinic acetylcholine receptor mutant using saccharomyces cerevisiae. | saccharomyces cerevisiae is a tractable yeast species for expression and coupling of heterologous g protein-coupled receptors with the endogenous pheromone response pathway. although this platform has been used for ligand screening, no studies have probed its ability to predict novel pharmacology and functional selectivity of allosteric ligands. as a proof of concept, we expressed a rat m(3) muscarinic acetylcholine receptor (machr) bearing a mutation (k(7.32)e) recently identified to confer pos ... | 2010 | 20466821 |
| carboxypeptidase e cytoplasmic tail mediates localization of synaptic vesicles to the pre-active zone in hypothalamic pre-synaptic terminals. | how synaptic vesicles (svs) are localized to the pre-active zone (5-200 nm beneath the active zone) in the nerve terminal, which may represent the slow response sv pool, is not fully understood. electron microscopy revealed the number of svs located in the pre-active zone, was significantly decreased in hypothalamic neurons of carboxypeptidase e knockout (cpe-ko) mice compared with wild-type mice. additionally, we found k(+)-stimulated glutamate secretion from hypothalamic embryonic neurons was ... | 2010 | 20492353 |
| transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides. | fusion proteins made up of glucagon-like peptide 1 (glp-1) and exendin-4 (ex-4) fused to a nonglycosylated form of human transferrin (glp-1-tf or ex-4-tf) were produced and characterized. glp-1-tf activated the glp-1 receptor, was resistant to inactivation by peptidases, and had a half-life of approximately 2 days, compared with 1 to 2 min for native glp-1. glp-1-tf retained the acute, glucose-dependent insulin-secretory properties of native glp-1 in diabetic animals and had a profound effect on ... | 2010 | 20498254 |
| phosphate activated glutaminase-like immunoreactivity in the nervous system from different species and in different neuronal cell types and in astrocytes. | using antibodies against pig brain phosphate activated glutaminase, the enzyme appears to be rather conservative as we have observed immuno- staining in the brain from all species investegated [pig, cow, rabbit, rat, mouse, man, fish (cod and salmon) and bird (chicken)]. in addition, phosphate activated glutaminase from cultured mouse cerebral cortex inter- neurons (mainly gaba-ergic), cerebellar granule cells (glutamatergic) and astrocytes stained in an analogous manner. however, no phosphate a ... | 1987 | 20501086 |
| mitochondria-targeted cytochrome p450 2e1 induces oxidative damage and augments alcohol-mediated oxidative stress. | the ethanol-inducible cytochrome p450 2e1 (cyp2e1) is also induced under different pathological and physiological conditions. studies including ours have shown that cyp2e1 is bimodally targeted to both the endoplasmic reticulum (microsomes) (mc cyp2e1) and mitochondria (mt cyp2e1). in this study we investigated the role of mtcyp2e1 in ethanol-mediated oxidative stress in stable cell lines expressing predominantly mt cyp2e1 or mc cyp2e1. the er+ mutation (a2l, a9l), which increases the affinity o ... | 2010 | 20529841 |
| alpha4 phosphoprotein interacts with edd e3 ubiquitin ligase and poly(a)-binding protein. | mammalian alpha4 phosphoprotein, the homolog of yeast tap42, is a component of the mammalian target-of-rapamycin (mtor) pathway that regulates ribogenesis, the initiation of translation, and cell-cycle progression. alpha4 is known to interact with the catalytic subunit of protein phosphatase 2a (pp2ac) and to regulate pp2a activity. using alpha4 as bait in yeast two-hybrid screening of a human k562 erythroleukemia cdna library, edd (e3 isolated by differential display) e3 ubiquitin ligase was id ... | 2010 | 20544796 |
| the production, characterisation and enhanced pharmacokinetics of scfv-albumin fusions expressed in saccharomyces cerevisiae. | an expression system is described for the production of monomeric scfvs and scfv antibody fragments genetically fused to human albumin (at either the n- or c-terminus or both). based upon strains of saccharomyces cerevisiae originally developed for the production of a recombinant human albumin (recombumin) this system has delivered high levels of secreted product into the supernatant of shake flask and high cell density fed-batch fermentations. specific binding to the corresponding ligand was de ... | 2010 | 20546898 |
| estrogenic pyrethroid pesticides regulate expression of estrogen receptor transcripts in mouse sertoli cells differently from 17beta-estradiol. | studies suggested that exposure to agricultural pesticides may affect male fertility. pyrethroids are widely used pesticides due to their insecticidal potency and low mammalian toxicity. a recombinant yeast assay system incorporating the human alpha-estrogen receptor was used to analyze the estrogenicity of a range of readily available pyrethroid pesticides. the commercial product ripcord plus showed estrogenic activity by this assay. to determine whether pyrethroid compounds might exert an effe ... | 2010 | 20574910 |
| preliminary safety assessment of yarrowia lipolytica extracellular lipase: results of acute and 28-day repeated dose oral toxicity studies in rats. | interest in extracellular lipase sourced from the non conventional yeast yarrowia lipolytica has increased over the last decade. the enzyme was recently suggested as a good candidate for pancreatic exocrine insufficiency treatment. however, there is still a lack of oral safety evaluation data. in this work, we conducted acute and 28-day repeated dose toxicity studies in rats. both male and female rats were first orally treated with fungal lipase at either single or repeated doses. the results de ... | 2010 | 20609427 |
| kbp interacts with scg10, linking goldberg-shprintzen syndrome to microtubule dynamics and neuronal differentiation. | goldberg-shprintzen syndrome (goshs) is a rare clinical disorder characterized by central and enteric nervous system defects. this syndrome is caused by inactivating mutations in the kinesin binding protein (kbp) gene, which encodes a protein of which the precise function is largely unclear. we show that kbp expression is up-regulated during neuronal development in mouse cortical neurons. moreover, kbp-depleted pc12 cells were defective in nerve growth factor-induced differentiation and neurite ... | 2010 | 20621975 |
| successful amelioration of mitochondrial optic neuropathy using the yeast ndi1 gene in a rat animal model. | leber's hereditary optic neuropathy (lhon) is a maternally inherited disorder with point mutations in mitochondrial dna which result in loss of vision in young adults. the majority of mutations reported to date are within the genes encoding the subunits of the mitochondrial nadh-quinone oxidoreductase, complex i. establishment of animal models of lhon should help elucidate mechanism of the disease and could be utilized for possible development of therapeutic strategies. | 2010 | 20628600 |
| glucocorticoid protection of oligodendrocytes against excitotoxin involving hypoxia-inducible factor-1alpha in a cell-type-specific manner. | glucocorticoids are commonly used in treating diseases with white matter lesions, including demyelinating diseases and spinal cord injury (sci). however, glucocorticoids are ineffective in gray matter injuries, such as head injury and stroke. the differential glucocorticoid effects in white and gray matter injuries are unclear. we report here a novel mechanism of methylprednisolone (mp), a synthetic glucocorticoid widely used for treating multiple sclerosis and sci, in protecting oligodendrocyte ... | 2010 | 20631191 |
| the role of autophagy during development in higher eukaryotes. | autophagy is a lysosome-mediated degradation pathway used by eukaryotes to recycle cytosolic components in both basal and stress conditions. several genes have been described as regulators of autophagy, many of them being evolutionarily conserved from yeast to mammals. the study of autophagy-defective model systems has made it possible to highlight the importance of correctly functioning autophagic machinery in the development of invertebrates as, for example, during the complex events of fly an ... | 2010 | 20633243 |
| pneumocystis carinii expresses an active rtt109 histone acetyltransferase. | pneumocystis (pc) remains a severe cause of pneumonia in immune compromised hosts. identification of specific targets present in pc, but lacking in humans, is paramount to developing new means to treat this infection. one such potential protein is rtt109, a unique histone acetyltransferase (hat) that acetylates h3 lysine 56 (h3-k56) in yeast and is required for dna replication. sequence homologues of rtt109 are present in other fungi, but are absent in mammals, making it a potential pan-specific ... | 2010 | 20656950 |
| the absence of genotoxicity of sucralose. | sucralose is a non-nutritive sweetener that is approximately 600 times sweeter than table sugar. it is currently approved for use in over 80 countries. evidence from chronic studies demonstrates that this compound is not carcinogenic. this report summarizes the results of genotoxicity studies that were part of the original safety assessment of sucralose-conducted early in the safety investigation and shared with regulatory agencies around the world. studies included the ames (salmonella typhimur ... | 2010 | 20691239 |
| evaluation of aqueous extract of felicia muricata leaves for anti-inflammatory, antinociceptive, and antipyretic activities. | felicia muricata thunb. (nees) (asteraceae) leaves are used in folklore medicine of south africa as an oral remedy for pain and inflammation. however, the efficacy of the plant part is yet to be validated with scientific experiments. | 2010 | 20731550 |
| marked amplification and diversification of products of ras genes from rat brain, rab gtpases, in the ciliates tetrahymena thermophila and paramecium tetraurelia. | small gtpase rab (products of ras genes from rat brain) is a widely conserved molecular switch among eukaryotes and regulates membrane trafficking pathways. it is generally considered that the number of rab encoded in the genome correlates with multicellularity; however, we found that unicellular ciliates tetrahymena thermophila (tt) and paramecium tetraurelia (pt) possess many more rab genes in their genome than the 64 hsrab genes in the human genome. we succeeded in isolating 86 cdna clones of ... | 2010 | 20738463 |
| biotin starvation with adequate glucose provision causes paradoxical changes in fuel metabolism gene expression similar in rat (rattus norvegicus), nematode (caenorhabditis elegans) and yeast (saccharomyces cerevisiae). | biotin affects the genetic expression of several glucose metabolism enzymes, besides being a cofactor of carboxylases. to explore how extensively biotin affects the expression of carbon metabolism genes, we studied the effects of biotin starvation and replenishment in 3 distantly related eukaryotes: yeast saccharomyces cerevisiae, nematode caenorhabditis elegans and rat rattus norvegicus. | 2010 | 20798549 |
| antioxidant rich grape pomace extract suppresses postprandial hyperglycemia in diabetic mice by specifically inhibiting alpha-glucosidase. | abstract: | 2010 | 20799969 |
| safety assessment of epa-rich oil produced from yeast: results of a 90-day subchronic toxicity study. | the safety of eicosapentaenoic acid (epa) oil produced from genetically modified yarrowia lipolytica yeast was evaluated following 90 days of exposure. groups of rats received 0 (olive oil), 98, 488, or 976 mg epa/kg/day, or gras fish oil or deionized water by oral gavage. rats were evaluated for in-life, neurobehavioral, anatomic and clinical pathology parameters. lower serum cholesterol (total and non-hdl) was observed in medium and high epa and fish oil groups. lower hdl was observed in high ... | 2010 | 20804805 |
| characterization of steroid receptor coactivator in sea urchin, strongylocentrotus nudus, and its involvement in embryonic development. | ligand-bound nuclear receptors (nrs) recruit coactivators such as members of the p160 steroid receptor coactivator (src) family and cyclic amp responsive element binding protein (creb)-binding protein (cbp) to specific enhancer elements and activate target gene transcription. in the present study, we isolated a novel src from the sea urchin strongylocentrotus nudus (snsrc) by using the ligand-binding domain of retinoid x receptor as a bait in a yeast two-hybrid screening. the snsrc and vertebrat ... | 2011 | 20816915 |
| a heuristic model for paradoxical effects of biotin starvation on carbon metabolism genes in the presence of abundant glucose. | we recently showed that in biotin starvation in yeast saccharomyces cerevisiae, nematode caenorhabditis elegans and rat rattus norvegicus, despite abundant glucose provision, the expression of genes for glucose utilization and lipogenesis were lowered, and for fatty acid β-oxidation and gluconeogenesis were raised, and glycolytic/fermentative flow was reduced. this work explored the mechanisms of these results. we show that they are associated with atp deficit and activation of the energy stress ... | 2010 | 20869286 |
| rap1gap interacts with ret and suppresses gdnf-induced neurite outgrowth. | glial cell line-derived neurotrophic factor (gdnf) was originally recognized for its ability to promote survival of midbrain dopaminergic neurons, but it has since been demonstrated to be crucial for the survival and differentiation of many neuronal subpopulations, including motor neurons, sympathetic neurons, sensory neurons and enteric neurons. to identify possible effectors or regulators of gdnf signaling, we performed a yeast two-hybrid screen using the intracellular domain of ret, the commo ... | 2010 | 20877310 |
| identification of novel species-selective agonists of the g-protein-coupled receptor gpr35 that promote recruitment of β-arrestin-2 and activate gα13. | the poorly characterized g-protein-coupled receptor gpr35 has been suggested as a potential exploratory target for the treatment of both metabolic disorders and hypertension. it has also been indicated to play an important role in immune modulation. a major impediment to validation of these concepts and further study of the role of this receptor has been a paucity of pharmacological tools that interact with gpr35. using a receptor-β-arrestin-2 interaction assay with both human and rat orthologue ... | 2010 | 20919992 |
| the beta1 subunit of the na,k-atpase pump interacts with megalencephalic leucoencephalopathy with subcortical cysts protein 1 (mlc1) in brain astrocytes: new insights into mlc pathogenesis. | megalencephalic leucoencephalopathy with subcortical cysts (mlc) is a rare congenital leucodystrophy caused by mutations in mlc1, a membrane protein of unknown function. mlc1 expression in astrocyte end-feet contacting blood vessels and meninges, along with brain swelling, fluid cysts and myelin vacuolation observed in mlc patients, suggests a possible role for mlc1 in the regulation of fluid and ion homeostasis and cellular volume changes. to identify mlc1 direct interactors and dissect the mol ... | 2010 | 20926452 |
| applying genetics and molecular biology to the study of the human pathogen cryptococcus neoformans. | the basidiomycete yeast crytococcus neoformans is a prominent human pathogen. it primarily infects immunocompromised individuals producing a meningoencephalitis that is lethal if untreated. recent advances in its genetics and molecular biology have made it a model system for understanding both the basidiomycota phylum and mechanisms of fungal pathogenesis. the relative ease of experimental manipulation coupled with the development of murine models for human disease allow for powerful studies in ... | 2010 | 20946836 |
| the antibiotic gentamicin inhibits specific protein trafficking functions of the arf1/2 family of gtpases. | gentamicin is a highly efficacious antibiotic against gram-negative bacteria. however, its usefulness in treating infections is compromised by its poorly understood renal toxicity. toxic effects are also seen in a variety of other organisms. while the yeast saccharomyces cerevisiae is relatively insensitive to gentamicin, mutations in any one of ∼20 genes cause a dramatic decrease in resistance. many of these genes encode proteins important for translation termination or specific protein-traffic ... | 2010 | 20956596 |
| the effect on rats of a diet with a high content of protein derived from food yeast. | 1946 | 20985911 | |
| regulation of m₃ muscarinic receptor expression and function by transmembrane protein 147. | the m₃ muscarinic acetylcholine receptor (m3r) regulates many fundamental physiological functions. to identify novel m3r-interacting proteins, we used a recently developed yeast two-hybrid screen (split ubiquitin method) to detect interactions among membrane proteins. this screen led to the identification of many novel m3r-associated proteins, including the putative membrane protein transmembrane protein 147 (tmem147). the amino acid sequence of tmem147 is highly conserved among mammals, but its ... | 2010 | 21056967 |
| antipyretic and antioxidant activities of 5-trifluoromethyl-4,5-dihydro-1h-pyrazoles in rats. | the objective of this study was to determine the effect of eight 5-hydroxy-5-trifluoromethyl-4,5-dihydro-1h-1-carboxyamidepyrazoles (tfdps) on rat body temperature and baker's yeast-induced fever. tfdps or vehicle (5% tween 80 in 0.9% nacl, 5 ml/kg) were injected subcutaneously and rectal temperature was measured as a function of time in 28-day-old male wistar rats (n = 5-12 per group). antipyretic activity was determined in feverish animals injected with baker's yeast (saccharomyces cerevisiae ... | 2010 | 21140097 |
| mammalian mthfd2l encodes a mitochondrial methylenetetrahydrofolate dehydrogenase isozyme expressed in adult tissues. | previous studies in our laboratory showed that isolated, intact adult rat liver mitochondria are able to oxidize the 3-carbon of serine and the n-methyl carbon of sarcosine to formate without the addition of any other cofactors or substrates. conversion of these 1-carbon units to formate requires several folate-interconverting enzymes in mitochondria. the enzyme(s) responsible for conversion of 5,10-methylene-tetrahydrofolate (ch(2)-thf) to 10-formyl-thf in adult mammalian mitochondria are curre ... | 2010 | 21163947 |
| protective role of raav-ndi1, serotype 5, in an acute mptp mouse parkinson's model. | defects in mitochondrial proton-translocating nadh-quinone oxidoreductase (complex i) have been implicated in a number of acquired and hereditary diseases including leigh's syndrome and more recently parkinson's disease. a limited number of strategies have been attempted to repair the damaged complex i with little or no success. we have recently shown that the non-proton-pumping, internal nadh-ubiquinone oxidoreductase (ndi1) from saccharomyces cerevisiae (baker's yeast) can be successfully inse ... | 2010 | 21188192 |
| antipyretic and antibacterial activity of chloranthus erectus (buch.-ham.) verdcourt leaf extract: a popular folk medicine of arunachal pradesh. | the main objective of this work was to study the antipyretic and antibacterial activity of c. erectus (buch.-ham.) verdcourt leaf extract in an experimental albino rat model. | 2010 | 21206616 |
| ubiquitin-dependent proteasomal degradation of human liver cytochrome p450 2e1: identification of sites targeted for phosphorylation and ubiquitination. | human liver cyp2e1 is a monotopic, endoplasmic reticulum-anchored cytochrome p450 responsible for the biotransformation of clinically relevant drugs, low molecular weight xenobiotics, carcinogens, and endogenous ketones. cyp2e1 substrate complexation converts it into a stable slow-turnover species degraded largely via autophagic lysosomal degradation. substrate decomplexation/withdrawal results in a fast turnover cyp2e1 species, putatively generated through its futile oxidative cycling, that inc ... | 2011 | 21209460 |
| antiinflammatory, analgesic and antipyretic activities of mimusops elengi linn. | in the present study, 70% ethanol extract of mimusops elengi linn. bark was assessed for antiinflammatory, analgesic and antipyretic activities in animals. the antiinflammatory activity of ethanol extract of mimusops elengi (200 mg/kg, p.o) was evaluated using carrageenan-induced paw edema and cotton pellet-induced granuloma models. analgesic effect was evaluated using acetic acid-induced writhing and eddy's hot plate models and antipyretic activity was assessed by brewer's yeast-induced pyrexia ... | 2010 | 21218059 |
| peptides and aptamers targeting hsp70: a novel approach for anticancer chemotherapy. | the inhibition of heat shock protein 70 (hsp70) is an emerging strategy in cancer therapy. unfortunately, no specific inhibitors are clinically available. by yeast two-hybrid screening, we have identified multiple peptide aptamers that bind hsp70. when expressed in human tumor cells, two among these peptide aptamers-a8 and a17-which bind to the peptide-binding and the atp-binding domains of hsp70, respectively, specifically inhibited the chaperone activity, thereby increasing the cells' sensitiv ... | 2011 | 21224349 |
| zinc-finger protein 90 negatively regulates neuron-restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes. | neuron-restrictive silencer factor (nrsf) is a zinc-finger transcription factor that binds to specific dna sequences (nrse) to repress transcription. by down-regulating the transcription of its target genes, nrsf contributes to the regulation of various biological processes, including neuronal differentiation, carcinogenesis and cardiovascular homeostasis. we previously reported that nrsf regulates expression of the cardiac fetal gene program, and that attenuation of nrsf-mediated repression con ... | 2011 | 21284946 |
| nutrient stress does not cause retrograde transport of cytoplasmic trna to the nucleus in evolutionarily diverse organisms. | intracellular trafficking of trna was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. this view has recently been challenged, however, by the discovery that trna can move retrograde from the cytoplasm back to the nucleus in saccharomyces cerevisiae and rat hepatoma h4iie cells during nutrient stress and in s. cerevisiae after intron-containing pre-trnas are spliced in the cytoplasm. contrary to studies reported, we prese ... | 2011 | 21289100 |
| [effect of beta-carotene and carotene producing yeast phaffia rhodozyma on oxidative stress in rats treated with tetrachloromethane]. | supplementation of rats' diet with beta-carotene or biomass of carotene producing yeast phaffia rhodozyma caused a decrease of aminotransferases in the blood serum as well as a decrease of lipid peroxidation products and protein carbonil groups in the liver, brain and myocardium tissues of animals treated with tetrachloromethane. when compared to the control group the activity of superoxide dismutase, catalase and glutathione peroxidase in the liver of carotene fed rats were respectively 1.6, 2. ... | 2010 | 21328879 |
| distinct regulation of p53-mediated apoptosis by protein kinase ca, d, e and ?: evidence in yeast for transcription-dependent and -independent p53 apoptotic mechanisms. | the role of individual protein kinase c (pkc) isoforms in the regulation of p53-mediated apoptosis is still uncertain. using yeast cells co-expressing the human wild-type p53 and a single mammalian pkca, d, e or ?, we showed a differential regulation of p53-mediated apoptosis by these pkc isoforms. whereas pkca and ? had no effect on p53 activity, pkcd and e stimulated a p53-mediated mitochondria-dependent apoptosis. moreover, using pifithrin-a and -µ, selective inhibitors of p53 transcriptional ... | 2011 | 21338602 |
| development of chromanes as novel inhibitors of the uncoupling proteins. | the uncoupling proteins (ucps) are mitochondrial carriers that modulate the energetic efficiency and, as a result, can lower superoxide levels. here, we describe the discovery of a small-molecule inhibitor of the ucps. screening of potential ucp1 regulators led to the identification of chromane derivatives that inhibit its proton conductance. members of the ucp family can act as a defense against oxidative stress and, thus, ucp2 plays a protective role in tumor cells. high ucp2 levels have been ... | 2011 | 21338923 |
| xenotransplantation of mitochondrial electron transfer enzyme, ndi1, in myocardial reperfusion injury. | a significant consequence of ischemia/reperfusion (i/r) is mitochondrial respiratory dysfunction, leading to energetic deficits and cellular toxicity from reactive oxygen species (ros). mammalian complex i, a nadh-quinone oxidoreductase enzyme, is a multiple subunit enzyme that oxidizes nadh and pumps protons across the inner membrane. damage to complex i leads to superoxide production which further damages complex i as well as other proteins, lipids and mtdna. the yeast, s. cerevisiae, expresse ... | 2011 | 21339825 |
| pharmacokinetic-pharmacodynamic modeling of the inhibitory effects of naproxen on the time-courses of inflammatory pain, fever, and the ex vivo synthesis of txb2 and pge2 in rats. | to quantify and compare the time-course and potency of the analgesic and antipyretic effects of naproxen in conjunction with the inhibition of pge(2) and txb(2). | 2011 | 21347567 |
| interaction of selenite and tellurite with thiol-dependent redox enzymes: kinetics and mitochondrial implications. | the interactions of selenite and tellurite with cytosolic and mitochondrial thioredoxin reductases (trxr1 and trxr2) and glutathione reductases (gr) from yeast and mammalian sources were explored. both trxr1 and trxr2 act as selenite and tellurite reductases. kinetic treatment shows that selenite has a greater affinity than tellurite with both trxr1 and trxr2. considering both k(cat) and k(m), selenite shows a better catalytic efficiency than tellurite with trxr1, whereas with trxr2, the catalyt ... | 2011 | 21397686 |
| a role for zo-1 and plekha7 in recruiting paracingulin to tight and adherens junctions of epithelial cells. | paracingulin is a 160-kda protein localized in the cytoplasmic region of epithelial tight and adherens junctions, where it regulates rhoa and rac1 activities by interacting with guanine nucleotide exchange factors. here, we investigate the molecular mechanisms that control the recruitment of paracingulin to cell-cell junctions. we show that paracingulin forms a complex with the tight junction protein zo-1, and the globular head domain of paracingulin interacts directly with zo-1 through an n-ter ... | 2011 | 21454477 |
| in vivo activity of sapindus saponaria against azole-susceptible and -resistant human vaginal candida species. | abstract: | 2011 | 21542936 |
| analysis of environmental endocrine disrupting activities in wastewater treatment plant effluents using recombinant yeast assays incorporated with exogenous metabolic activation system. | to measure the endocrine disrupting chemicals (edcs) in wastewater and evaluate the edcs removal efficiencies in the municipal wastewater treatment plants (wwtp). | 2011 | 21565684 |
| pinta: a web server for network-based gene prioritization from expression data. | pinta (available at http://www.esat.kuleuven.be/pinta/; this web site is free and open to all users and there is no login requirement) is a web resource for the prioritization of candidate genes based on the differential expression of their neighborhood in a genome-wide protein-protein interaction network. our strategy is meant for biological and medical researchers aiming at identifying novel disease genes using disease specific expression data. pinta supports both candidate gene prioritization ... | 2011 | 21602267 |
| structure-activity relationships influencing lipid-induced changes in eif2α phosphorylation and cell viability in brin-bd11 cells. | fatty acids influence the viability of eukaryotic cells differentially such that long chain saturated molecules are poorly tolerated, whereas unsaturated species are less detrimental and can be cytoprotective. the basis for these effects is unclear but studies in yeast imply that they reflect the spatial configuration of the molecules when incorporated into the er membrane. using brin-bd11 β-cells, we show that a wide range of unsaturated free fatty acids and their methyl-esters (having differin ... | 2011 | 21627968 |
| yapsin 1 immunoreactivity in alpha cells of human pancreatic islets: implications for the processing of human proglucagon by mammalian aspartic proteases. | yapsin 1 is an aspartic protease from saccharomyces cerevisiae and belongs to a class of aspartic proteases that demonstrate specificity for basic amino acids. it is capable of processing prohormone substrates at specific basic residue cleavage sites, similar to that of the prohormone convertases, to generate bioactive peptide hormones. an antibody raised against yapsin 1 was previously shown to immuno-stain endocrine cells of rat pituitary and brain as well as lysates from bovine pituitary secr ... | 2011 | 21632904 |
| loco signaling pathway in longevity. | despite the various roles of regulator of g protein signaling (rgs) protein in the g protein signaling pathway that have been defined, the function of rgs has not been characterized in longevity signaling pathways. we found that reduced expression of loco, a drosophila rgs protein, resulted in a longer lifespan of flies with stronger resistance to stress, higher mnsod activity and increased fat content. in contrast, overexpression of the loco gene shortened the fly lifespan significantly, lowere ... | 2011 | 21776417 |
| the wd40 repeat ptdins(3)p-binding protein epg-6 regulates progression of omegasomes to autophagosomes. | ptdins(3)p plays critical roles in the autophagy pathway. however, little is known about how ptdins(3)p effectors act with autophagy proteins in autophagosome formation. here we identified an essential autophagy gene in c. elegans, epg-6, which encodes a wd40 repeat-containing protein with ptdins(3)p-binding activity. epg-6 directly interacts with atg-2. epg-6 and atg-2 regulate progression of omegasomes to autophagosomes, and their loss of function causes accumulation of enlarged early autophag ... | 2011 | 21802374 |
| the n-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor. | for the development of a biomimetic odor-sensing system, we investigated the effects of replacing the n-terminus of an olfactory receptor (or) on its functional expression in the budding yeast, saccharomyces cerevisiae. using the mouse olfactory receptor or226 (mor226), three types of chimeric ors were constructed by replacing n-terminal regions of mor226 with the corresponding regions of the rat i7 receptor, which is known to be functionally expressed in yeast. the replacement of the n-terminal ... | 2012 | 21915853 |
| glutamine analogs promote cytoophidium assembly in human and drosophila cells. | ctp synthase is compartmentalized within a subcellular structure, termed the cytoophidium, in a range of organisms including bacteria, yeast, fruit fly and rat. here we show that ctp synthase is also compartmentalized into cytoophidia in human cells. surprisingly, the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-l-norleucine (don), an inhibitor of glutamine-dependent enzymes including ctp synthase. experiments in flies confirmed that don ... | 2011 | 21930098 |
| ubiquitin ligase nedd4 promotes alpha-synuclein degradation by the endosomal-lysosomal pathway. | α-synuclein is an abundant brain protein that binds to lipid membranes and is involved in the recycling of presynaptic vesicles. in parkinson disease, α-synuclein accumulates in intraneuronal inclusions often containing ubiquitin chains. here we show that the ubiquitin ligase nedd4, which functions in the endosomal-lysosomal pathway, robustly ubiquitinates α-synuclein, unlike ligases previously implicated in its degradation. purified nedd4 recognizes the carboxyl terminus of α-synuclein (residue ... | 2011 | 21953697 |
| The plasma membrane-associated GTPase Rin interacts with the dopamine transporter and is required for protein kinase C-regulated dopamine transporter trafficking. | Dopaminergic signaling and plasticity are essential to numerous CNS functions and pathologies, including movement, cognition, and addiction. The amphetamine- and cocaine-sensitive dopamine (DA) transporter (DAT) tightly controls extracellular DA concentrations and half-life. DAT function and surface expression are not static but are dynamically modulated by membrane trafficking. We recently demonstrated that the DAT C terminus encodes a PKC-sensitive internalization signal that also suppresses b ... | 2011 | 21957239 |
| Functional links between Aß toxicity, endocytic trafficking, and Alzheimer's disease risk factors in yeast. | Aß (beta-amyloid peptide) is an important contributor to Alzheimer's disease (AD). We modeled Aß toxicity in yeast by directing the peptide to the secretory pathway. A genome-wide screen for toxicity modifiers identified the yeast homolog of phosphatidylinositol binding clathrin assembly protein (PICALM) and other endocytic factors connected to AD whose relationship to Aß was previously unknown. The factors identified in yeast modified Aß toxicity in glutamatergic neurons of Caenorhabditis elega ... | 2011 | 22033521 |
| schizosaccharomyces pombe, unlike saccharomyces cerevisiae, may not directly regulate nuclear-cytoplasmic transport of spliced trnas in response to nutrient availability. | eukaryotic cells adapt to changes in nutrient levels by regulating key processes, such as gene transcription, ribosome biogenesis, and protein translation. several studies have shown that nuclear export of trnas is also regulated in saccharomyces cerevisiae and rat hepatoma h4iie cells during nutrient stress. however, recent studies suggest that nutrient stress does not affect nuclear trna export in several mammalian cell lines, including rat hepatoma h4iie. furthermore, in contrast to previous ... | 2011 | 22077425 |
| elevated levels of renal and circulating nop-7-associated 2 (nsa2) in rat and mouse models of diabetes, in mesangial cells in vitro and in patients with diabetic nephropathy. | aims/hypothesis: we previously found that nop-7-associated 2 (nsa2), which is involved in ribosomal biogenesis in yeast and is a putative cell cycle regulator in mammalian cells, is elevated in the kidney of goto-kakizaki (gk) rat, a spontaneous model of type 2 diabetes. here we tested the hypothesis that elevated nsa2 is involved in diabetic nephropathy (dn). methods: we examined nsa2/nsa2 expression and nsa2 pr ... | 2011 | 22095236 |
| map4 mechanism that stabilizes mitochondrial permeability transition in hypoxia: microtubule enhancement and dynlt1 interaction with vdac1. | mitochondrial membrane permeability has received considerable attention recently because of its key role in apoptosis and necrosis induced by physiological events such as hypoxia. the manner in which mitochondria interact with other molecules to regulate mitochondrial permeability and cell destiny remains elusive. previously we verified that hypoxia-induced phosphorylation of microtubule-associated protein 4 (map4) could lead to microtubules (mts) disruption. in this study, we established the hy ... | 2011 | 22164227 |
| preparation, characterization, and biological properties of β-glucans. | β-glucans are soluble fibers with physiological functions, such as, interference with absorption of sugars and reduction of serum lipid levels. β-glucans are found in different species, such as, rhynchelytrum repens, lentinus edodes, grifola frondosa, tremella mesenterica, tremella aurantia, zea may, agaricus blazei, phellinus baummi, saccharomyces cerevisae (yeast), and agaricus blazei murell (mushroom). analysis of the fractions reveals the presence of arabinose, glucose, xylose, and traces of ... | 2011 | 22171300 |
| safety evaluation of a proprietary food-grade, dried fermentate preparation of saccharomyces cerevisiae. | a safety evaluation was performed for epicor, a product produced by a proprietary fermentation process using saccharomyces cerevisiae. studies included the following assays: bacterial reverse mutation, mouse lymphoma cell mutagenicity, mitogenicity assay in human peripheral lymphocytes, and a cytochrome p450 ([cyp] cyp1a2 and cyp3a4) induction assessment as well as 14-day acute, 90-day subchronic, and 1-year chronic oral toxicity studies in rats. no evidence of genotoxicity or mitogenicity was s ... | 2012 | 22222580 |
| comparison of two expression systems using cos7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1. | carnitine palmitoyltransferase 1 (cpt1), catalyzing the transfer of the acyl group from acyl-coa to carnitine to form acylcarnitine, is located at the outer mitochondrial membrane. because it is easily inactivated by solubilization, expression systems using living cells are essential for its functional characterization. cos7 cells or yeast cells are often utilized for this purpose; however, the advantages/disadvantages of the use of these cells or the question as to how the cpt1 enzyme expressed ... | 2012 | 22266133 |