Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human papillomavirus is a necessary cause of invasive cervical cancer worldwide. | a recent report that 93 per cent of invasive cervical cancers worldwide contain human papillomavirus (hpv) may be an underestimate, due to sample inadequacy or integration events affecting the hpv l1 gene, which is the target of the polymerase chain reaction (pcr)-based test which was used. the formerly hpv-negative cases from this study have therefore been reanalyzed for hpv serum antibodies and hpv dna. serology for hpv 16 vlps, e6, and e7 antibodies was performed on 49 of the 66 cases which w ... | 1999 | 10451482 |
| loss of retinoblastoma protein expression is frequent in small cell neuroendocrine carcinoma of the cervix and is unrelated to hpv type. | we have previously identified an inverse relationship between p53 and retinoblastoma protein (prb) immunoreactivity in non-small cell carcinoma of the cervix. because prb is infrequently expressed in small cell carcinoma of the lung, we analyzed 25 small cell neuroendocrine carcinomas of the cervix to test the hypotheses that 1) lack of prb expression is associated with the neuroendocrine phenotype in human papillomavirus (hpv)-associated cervical carcinoma and 2) the inverse relationship betwee ... | 1999 | 10452502 |
| the g(2) checkpoint is maintained by redundant pathways. | while p53 activity is critical for a dna damage-induced g(1) checkpoint, its role in the g(2) checkpoint has not been compelling because cells lacking p53 retain the ability to arrest in g(2) following dna damage. comparison between normal human foreskin fibroblasts (hffs) and hffs in which p53 was eliminated by transduction with human papillomavirus type 16 e6 showed that treatment with adriamycin initiated arrest in g(2) with active cyclin b/cdc2 kinase, regardless of p53 status. both e6-trans ... | 1999 | 10454534 |
| genetic susceptibility to hpv infection and cervical cancer. | squamous cell carcinoma of the cervix (sccc) is one of the leading causes of death in developing countries. infection with high-risk human papillomavirus (hpv) is the major risk factor to develop malignant lesions in the cervix. polymorphisms of the mhc and p53 genes seem to influence the outcome of hpv infection and progression to sccc, although controversial data have been reported. mhc are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in i ... | 1999 | 10454752 |
| the scymv1 hairpin ribozyme: targeting rules and cleavage of heterologous rna. | the catalytic center of the rna from the negative strand of the satellite rna of chicory yellow mottle virus type 1 (scymv1) is in the hairpin ribozyme family, has catalytic activity, and cleaves substrates before a preferred gua sequence. this is different from that of the satellite rna from the negative strand of tobacco ringspot virus (strsv) which prefers a guc sequence at the site of cleavage. the scymv1 hairpin ribozyme has now been developed for cleaving heterologous rna substrates. when ... | 1999 | 10455414 |
| cellular transcription factors regulate human papillomavirus type 16 gene expression by binding to a subset of the dna sequences recognized by the viral e2 protein. | human papillomavirus type 16 (hpv-16) is a dna tumour virus that has been implicated in the development of cervical cancer. the hpv-16 e2 protein binds to four sites that are present upstream of the viral p97 promoter and regulates transcription of the e6 and e7 oncogenes. here, it is shown that cellular transcription factors bind to two of these e2 sites. one cellular e2 site-binding factor, which is here named cef-1, binds tightly to e2 site 1. cef-2, an unrelated cellular e2 site-binding fact ... | 1999 | 10466807 |
| papillomavirus e2 induces p53-independent apoptosis in hela cells. | we have previously shown that expression of the papillomavirus e2 protein in hela cells induces p53 accumulation and causes both cell cycle arrest and apoptosis. in contrast to growth arrest, onset of apoptosis was not correlated with an increase of p53 transcriptional activity. in the present study, we conducted biochemical and genetic experiments in order to determine whether e2-induced apoptosis was independent of p53 induction. we showed that e2 did not alter the transcription of bax, a know ... | 1999 | 10467398 |
| induction of cellular immunosuppression by the human papillomavirus type 16 e7 oncogenic protein. | the human papillomavirus type 16 (hpv-16) e7 oncogenic protein is found in the culture supernatant of siha cells, a cervical carcinoma cell line. extracellular e7 protein, acting as a viral toxin in human immune cells, induces the overproduction of the immune suppressive ifn alpha cytokine by apcs, and inhibits the t-cell response to recall and allogenic antigens. these effects should be taken into account for the design of anti-human cervical carcinoma vaccines. | 1999 | 10472433 |
| th1 cytokine patterns in cervical human papillomavirus infection. | the host's immune response to cervical human papillomavirus (hpv) infection is poorly understood. in a longitudinal cohort of women with cervical hpv infections, defined by pcr-based hpv dna testing, we used exfoliated cervical cells and reverse transcription-pcr to examine the cervical mucosal mrna expression of cytokines involved in regulating cell-mediated immunity. we identified seven hpv-positive subjects who were found to have cleared their hpv infections 4 months later. in all seven, a t- ... | 1999 | 10473530 |
| characterization of the regulatory domains of the human skn-1a/epoc-1/oct-11 pou transcription factor. | the skn-1a pou transcription factor is primarily expressed in keratinocytes of murine embryonic and adult epidermis. although some pou factors expressed in a tissue-specific manner are important for normal differentiation, the biological function of skn-1a remains unknown. previous in vitro studies indicate that skn-1a has the ability to transactivate markers of keratinocyte differentiation. in this study, we have characterized skn-1a's transactivation domain(s) and engineered a dominant negativ ... | 1999 | 10473598 |
| characterization of immortalized rabbit lacrimal gland epithelial cells. | to establish an immortalized lacrimal gland epithelial cell line, the orbital lacrimal glands of normal new zealand white rabbits were multiply injected with an immortalizing amphotropic retroviral vector (lxsn16e6e7) containing the e6 and e7 genes of human papillomavirus type 16. lacrimal glands were removed after 2 d and acinar epithelial cells were isolated and cultured on matrigel-coated 60 mm2 plates containing dmem-f12 supplemented with 5% nu-serum v. transformed cells were selected in g41 ... | 1999 | 10478799 |
| allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix. | transitional cell carcinomas (tccs) of the cervix are rare neoplasms of the female genital tract. although these tumors display urothelial differentiation, there is controversy regarding their histogenetic relationship to squamous cell carcinomas (scc) of the cervix versus transitional cell carcinomas of the bladder. | 1999 | 10479494 |
| [human papillomavirus oncogenes and immortalization of cultured human cells]. | 1999 | 10483302 | |
| [cloning transforming genes from human papillomavirus type 18]. | nucleotide sequences of type 18 human papilloma virus genes e6 and e7 inserted in human dna cloned from cervical tumor are determined. the resultant sequences are compared to the prototype variant. five point mutations not leading to replacement of amino acid residues in polypeptide chain and 1 substituting the amino acid in codon 129 are detected in gene e6 sequence. in e7 sequence, one significant mutation in codon 92 is detected. both substitutions of asparagine for lysine are localized in th ... | 1998 | 10488529 |
| human papillomavirus type 16 and 18 detection in the management of mild dyskaryosis. | to determine if semi-quantitative human papillomavirus (hpv) types 16 and 18 detection by polymerase chain reaction can increase the sensitivity and specificity of repeat cytology alone for underlying high grade cervical intraepithelial neoplasia (cin). | 1999 | 10492111 |
| are benign cellular changes on a papanicolaou smear really benign? a prospective cohort study. | to determine the underlying prevalence of cervical intraepithelial neoplasia (cin) in women with benign cellular changes on a papanicolaou smear, and to evaluate follow-up strategies to identify women at high risk for serious underlying pathology. | 1999 | 10500518 |
| a broad range of human papillomavirus types detected with a general pcr method suitable for analysis of cutaneous tumours and normal skin. | a pair of degenerate pcr primers (fap59/64) was designed from two relatively conserved regions of the l1 open reading frame of most human papillomaviruses (hpv). the size of the generated amplicon was about 480 bp. pcr using these primers was found capable of amplifying dna from 87% (65/75) of the hpv types tested, its sensitivity being 1-10 copies for hpv-5, -20 and -30 clones. hpv was found in 63% (5/8) of tumour samples and in 63% (5/8) of normal skin biopsies from patients with various cutan ... | 1999 | 10501499 |
| transcription-modulatory activities of differentially spliced cdnas encoding the e2 protein of human papillomavirus type 16. | human papillomavirus (hpv) type 16 expresses a variety of alternatively spliced polycistronic mrnas encoding the e2 transcription-regulatory protein. these mrnas initiate at the p97 promoter and contain the 880/2708 (a-type), 880/2581 (a'-type) and 226/2708 (d-type) splice sites upstream from the e2 open reading frame (orf). recent studies investigating the translational capacities of partial cdnas representing three of these mrnas indicated their abilities to function in e2 protein translation, ... | 1999 | 10501502 |
| establishment of the human papillomavirus type 16 (hpv-16) life cycle in an immortalized human foreskin keratinocyte cell line. | the study of human papillomaviruses (hpvs) in cell culture has been hindered because of the difficulty in recreating the three-dimensional structure of the epithelium on which the virus depends to complete its life cycle. additionally, the study of genetic mutations in the hpv genome and its effects on the viral life cycle are difficult using the current method of transfecting molecularly cloned hpv genomes into early-passage human foreskin keratinocytes (hfks) because of the limited life span o ... | 1999 | 10502513 |
| screening for cancer: future potential. | much progress has been made in cancer screening over the past decade, but a great deal more needs to be done if screening is to make a major impact on worldwide cancer mortality. where fully implemented, cytological screening for cervical precursor lesions has had a major impact on mortality. however, the cost and required infrastructure levels are high, and new approaches are needed if screening is to be effective in the developing world. testing for the human papillomavirus and automated liqui ... | 1999 | 10505025 |
| detection of human papillomavirus (hpv) in laryngeal carcinoma cell lines provides evidence for a heterogeneic cell population. | the role of human papillomavirus (hpv) has been studied in laryngeal carcinomas with contradictory results. to evaluate the causal relationship between hpv infection and epithelial malignancies of the larynx, 27 laryngeal carcinoma cell lines from 22 patients were studied. also, paraffin-embedded biopsy samples of the original tumours were available from 12 patients. first, southern blot hybridisation (sbh) was used for the analysis of 18 cell lines and 12 original tumour sections were studied b ... | 1999 | 10505045 |
| the promoter of a novel human papillomavirus (hpv77) associated with skin cancer displays uv responsiveness, which is mediated through a consensus p53 binding sequence. | an aetiological role has been proposed for human papillomavirus (hpv) in skin carcinogenesis within the immunosuppressed patient population. to examine this possibility, we have focused on an hpv type that, to date, has been identified only in the cutaneous lesions of renal transplant recipients despite a high degree of sequence homology with other hpvs commonly found in warts in the general population. we report that the non-coding region of this virus, hpv type 77, contains a consensus binding ... | 1999 | 10508168 |
| molecular analysis of resistance to interferon in patients with laryngeal papillomatosis. | although interferon (ifn)-alpha has been used successfully as an adjuvant therapy in laryngeal papillomatosis, some patients are resistant to this treatment. in order to know which patients will benefit from the therapy, we have tried to find a relationship between the ifn response and the viral and host parameters in the lesion. detection of viral type and copy numbers by polymerase chain reaction (pcr) showed that all patients infected with human papillomavirus (hpv)-11 were sensitive to the t ... | 1999 | 10515680 |
| nasal immunization of mice with human papillomavirus type 16 (hpv-16) virus-like particles or with the hpv-16 l1 gene elicits specific cytotoxic t lymphocytes in vaginal draining lymph nodes. | human papillomavirus type 16 (hpv-16) infects the genital tract and is closely associated with the development of cervical cancer. hpv-16 initiates infection at the genital mucosal surface; thus, mucosal immune responses are likely to contribute to defense against hpv-16 infection. however, little information is available regarding the induction of immune responses in the genital tract mucosa. in this study, we evaluated the potential of intranasally administered papillomavirus vaccines to elici ... | 1999 | 10516012 |
| comparison of variant-specific hybridization and single-strand conformational polymorphism methods for detection of mixed human papillomavirus type 16 variant infections. | pcr-based variant-specific hybridization (vsh) and single-strand conformational polymorphism (sscp) analyses were compared for their capacities to detect mixed human papillomavirus type 16 (hpv-16) variant infections within clinical specimens. the sscp assay used in this comparison targets a 682-bp fragment that spans nucleotides 7445 to 222 within the hpv-16 genome. this fragment includes portions of the hpv-16 long control region and the e6 open reading frame and identifies three categories of ... | 1999 | 10523565 |
| comparative response of normal and of human papillomavirus-16 immortalized human epithelial cervical cells to benzo[a]pyrene. | laboratory evidence suggests synergism of human papillomavirus (hpv) infection with cigarette smoking behaviors in enhancing the risk of cervical cancer. in this preliminary investigation, we tested the hypothesis that hpv infection may alter the metabolic activation of tobacco smoke carcinogens, such as benzo[a]pyrene (b[a]p), thereby playing a role in the etiology of cervical cancer. we examined in vitro the metabolism and dna adduct formation of [3h]b[a]p in normal and hpv-16 immortalized hum ... | 1999 | 10523714 |
| [the role of oncogenic human papillomaviruses in tonsillar squamous cell carcinomas with functional inactivation of the retinoblastoma protein]. | in order to identify squamous cell carcinomas of the head and neck (hnscc) with common biological and clinical features, we investigated the incidence and properties of carcinomas lacking retinoblastoma protein (pr6) cell cycle control. of 208 hnscc investigated, 23 (11%) showed a lack of prb expression. the majority of these tumors (65%) were tonsillar carcinomas. the prb-negative tonsillar tumors were all stage iv, had metastasized to lymph nodes at the time of diagnosis and were in general po ... | 1999 | 10525608 |
| application of homogeneous time-resolved fluorescence (htrftm) to monitor poly-ubiquitination of wild-type p53. | rapid degradation of wild-type p53 in the human uterine cervix is induced by the infection of high-risk human papilloma virus (hpv) types 16 and 18. hpv-e6 protein plays a critical role in the poly-ubiquitination of wild-type p53 by mediating the association of p53 with e6-associated protein (e6ap). as a result, the poly-ubiquitinated p53 is rapidly and selectively degraded by the 26s proteasome. we have established a high throughput assay system to monitor poly-ubiquitination of wild-type p53 u ... | 1999 | 10539989 |
| aetiological parallel between tonsillar and anogenital squamous-cell carcinomas. | patients with human papillomavirus (hpv)-associated anogenital cancers had a 4.3-fold increased risk of tonsillar squamous-cell carcinoma. these cancer types also have histopathological and molecular biological similarities. thus hpv may be aetiologically important in tonsillar carcinogenesis. | 1999 | 10543674 |
| hpv 16 e6 blocks tnf-mediated apoptosis in mouse fibroblast lm cells. | the interaction between hosts and the viruses that infect them is a dynamic one, and a growing literature documents the fact that many viruses have developed mechanisms designed to avoid elimination by the host immune system. one of the immune strategies used by the host and targeted by virus proteins is apoptosis triggered by the cytokine tumor necrosis factor (tnf). mouse fibroblast lm cells are spontaneously sensitive to tnf. when the wild-type e6 protein from the human papillomavirus type 16 ... | 1999 | 10544129 |
| seroprevalence of human papillomavirus type 16 in pregnant women. | to determine the seroprevalence of and risk factors for human papillomavirus (hpv) type 16 capsid antibodies in a large cohort of pregnant women. | 1999 | 10546705 |
| screening for squamous intraepithelial lesions with fluorescence spectroscopy. | to evaluate the accuracy of fluorescence spectroscopy in screening for squamous intraepithelial lesions (sils) and to compare its performance with that of papanicolaou smear screening, colposcopy, cervicoscopy, cervicography, and human papillomavirus (hpv) testing. | 1999 | 10546779 |
| cell transformation by the e7 oncoprotein of human papillomavirus type 16: interactions with nuclear and cytoplasmic target proteins. | the e7 oncoprotein of human papillomavirus type 16 (hpv-16) has long been known as a potent immortalizing and transforming agent. however, the molecular mechanisms underlying cell transformation and immortalization by e7 remain largely unknown. it is believed that e7 exerts its oncogenic function at least in part by modulating cellular growth regulatory pathways. increasing experimental evidence suggests that cell transformation by e7 is mediated by the physical association of e7 with cellular r ... | 2000 | 10547667 |
| high prevalence of human papillomaviruses in the normal oral cavity of adults. | human papillomavirus (hpv) infection in the normal oral cavity was studied by the sensitive polymerase chain reaction (pcr) using primers for the l1 region of human papillomavirus dna and high fidelity amplification system. cells were scraped from the oral mucosae of 7 (mean age; 42 years) and 30 (mean age; 32 years) volunteers with and without skin warts, respectively. human papillomavirus dna was detected in 30/37 (81.1%) specimens and their copy numbers per cell were 10(-1) to 10(-4) (mean, 1 ... | 1999 | 10551162 |
| human papillomavirus in squamous metaplastic epithelium with dysplasia of the epididymis detected by pcr method. | 1999 | 10555017 | |
| detection of the e7 transform gene of human papilloma virus type 16 in human oral squamous cell carcinoma. | to determine, with the use of polymerase chain reaction, the prevalence of human papillomavirus (hpv) 16 in 30 patients with primary oral squamous cell carcinoma (oscc) and 30 healthy control patients. | 1998 | 10557171 |
| detection of adeno-associated virus in human semen: does viral infection play a role in the pathogenesis of male infertility? | to evaluate the occurrence of adeno-associated virus (aav) dna and/or human papillomavirus (hpv) dna in the semen of infertile men as a possible factor in the pathogenesis of male infertility. | 1999 | 10560983 |
| genital human papillomavirus infection among women recruited for routine cervical cancer screening or for colposcopy determined by hybrid capture ii and polymerase chain reaction. | the purpose of this study was to evaluate the clinical use of the hybrid capture (hc)-ii system for the detection of human papillomavirus (hpv) dna to identify women at risk of progression to high grade squamous intraepithelial lesions (hgsil) and carcinomas by differentiating low risk (lr) hpv types (6, 11, 42, 43, 44) and high/intermediate risk (hr) hpv types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68). five hundred and ninety-six women were enrolled in the study. among them, 466 atte ... | 1999 | 10565688 |
| pcr-rflp-detected human papilloma virus infection in a group of senegalese women attending an std clinic and identification of a new hpv-68 subtype. | cancer of the cervix is the most common malignant tumor among women in africa and, in particular, senegal. studies of the prevalence of human papilloma virus (hpv) infection in africa have mainly focused on carcinomas. data on the presence of the virus in women with normal cervical cytology are scarce. in this study, 158 cytologically normal women who had been referred to the 'institut pasteur de dakar' (senegal) for various genital complaints were investigated for the presence of hpv on exfolia ... | 1999 | 10567840 |
| favorable clinical outcome of cervical cancers infected with human papilloma virus type 58 and related types. | to determine whether the status of human-papillomavirus (hpv) infection affects the clinical outcome of cervical carcinoma (cc), hpv genotype was prospectively determined in 94 consecutive cc cases subsequently followed for a median duration of 37.5 months. with a consensus pcr-rflp method of hpv genotyping, 81 (86.2%) cancers were positive for hpv dna. they were classified, according to the phylogenic similarities, into hpv-16-related (type 16, n = 45; type 31, n = 2), hpv-58-related (type 58, ... | 1999 | 10567897 |
| penile cancer among patients with genital lichen sclerosus. | genital lichen sclerosus (ls) has sporadically been reported to be associated with penile squamous cell carcinoma (scc). | 1999 | 10570372 |
| low levels of serum vitamins a and e in blood and subsequent risk for cervical cancer: interaction with hpv seropositivity. | nutritional factors have been associated with risk of cervical cancer, but it is unclear whether the associations are of etiological significance or secondary to human papillomavirus (hpv) exposure. a delineation of this question requires a prospective study with invasive cancer as the end point. we conducted a nested case-control study in finland and sweden within a joint cohort of 405,000 women followed up for, on average, 4 years. blood samples from 38 prospective cases of invasive cervical c ... | 1999 | 10578492 |
| serological evidence for protection by human papillomavirus (hpv) type 6 infection against hpv type 16 cervical carcinogenesis. | human papillomavirus (hpv) exists as more than 100 genotypes. it is not well-established whether the different hpv types interfere with infection or pathogenesis by each other. possible interactions in cervical carcinogenesis between infection with the most common hpv types (6, 11, 16, 18 and 33) were studied in a seroepidemiological case- control study of 218 women with primary untreated cervical cancer and 219 healthy age-matched control women. as previously shown, hpv-16 seropositivity was as ... | 1999 | 10580926 |
| serum carotenoids and vitamins and risk of cervical dysplasia from a case-control study in japan. | the relationships between risk of cervical dysplasia and dietary and serum carotenoids and vitamins were investigated in a case-control study. cases were 156 women who attended papanicolaou test screening in nine institutes affiliated with japan study group of human papillomavirus (hpv) and cervical cancer and had cervical dysplasia newly histologically confirmed. age-matched controls were selected from women with normal cervical cytology attending the same clinic. blood sample and cervical exfo ... | 1999 | 10584887 |
| magnesium ions enhance the transfer of human papillomavirus e2 protein from non-specific to specific binding sites. | the human papillomavirus 16 e2 protein binds to four specific dna sequences present within the hpv 16 genome and regulates viral gene expression and dna replication. however, the e2 protein can also bind tightly to non-specific dna sequences. here, we show that in binding reactions which contain an excess of non-specific dna, magnesium ions enhance the binding of e2 to its specific sites. in contrast, in the absence of non-specific dna, magnesium ions have no effect on the binding of e2 to speci ... | 1999 | 10588894 |
| the human papillomavirus type 16 e5 protein modulates phospholipase c-gamma-1 activity and phosphatidyl inositol turnover in mouse fibroblasts. | the human papillomavirus type 16 e5 (hpv16-e5) protein is a membrane protein that has been associated with malignant growth. the protein affects growth factor-mediated signal transduction in a ligand-dependent manner. we show now that e5 expression in a31 fibroblasts results in an increased level of diacylglycerol (dag) and inositol phosphates. immunoprecipitation of phospholipase c-gamma-1 (plc-gamma-1) with specific antibodies and immunoblotting with anti-phosphotyrosine antibodies reveal a la ... | 1999 | 10597278 |
| impaired nucleotide excision repair in uv-irradiated human oral keratinocytes immortalized with type 16 human papillomavirus genome. | we previously reported that 'high risk' human papillomaviruses (hpv) induce genetic instability in human oral keratinocytes. to understand the mechanisms of hpv-induced genetic instability, we determined the nucleotide excision repair (ner) capacity of normal (nhok) and human papillomavirus type-16 immortalized oral keratinocytes (hok-16b) by strand-specific removal of uv-induced cyclobutane pyrimidine dimers (cpds) from a 16 kb fragment of the p53 gene. in nhok the ner activity was initiated in ... | 1999 | 10597299 |
| synthesis of ifn-gamma by cd8(+) t cells is preserved in hiv-infected women with hpv-related cervical squamous intraepithelial lesions. | the aim of this study was to investigate whether coinfection with hiv affects the synthesis of th1 and th2 cytokines by peripheral blood t cells of women infected with human papillomavirus (hpv). | 1999 | 10600293 |
| naturally occurring, nonregressing canine oral papillomavirus infection: host immunity, virus characterization, and experimental infection. | papillomaviruses occasionally cause severe, nonregressing or recurrent infections in their human and animal hosts. the mechanisms underlying these atypical infections are not known. canine oral papillomavirus (copv) typically regresses spontaneously and is an important model of mucosal human papillomavirus infections. a severe, naturally occurring, nonregressing copv infection provided an opportunity to investigate some aspects of viral pathogenicity and host immunity. in this case, the papillom ... | 1999 | 10600607 |
| human papillomavirus and the risk of cervical cancer. | human papillomavirus (hpv) is now recognized as the driving force behind the dysregulation of the cell cycle that underlies cervical malignant transformation. although nearly all cervical cancers contain hpv genomes, the vast majority of hpvs are not oncogenic but merely induce benign lesions. because progressive abnormalities take at least a decade to develop into invasive cancers, young women with low-grade lesions can often be managed with watchful waiting rather than ablative therapy. | 1999 | 10616548 |
| dwarfism and dysregulated proliferation in mice overexpressing the myc antagonist mad1. | the four members of the mad family are bhlhzip proteins that heterodimerize with max and act as transcriptional repressors. the switch from myc-max complexes to mad-max complexes has been postulated to couple cell-cycle arrest with differentiation. the ectopic expression of mad1 in transgenic mice led to early postnatal lethality and dwarfism and had a profound inhibitory effect on the proliferation of the hematopoietic cells and embryonic fibroblasts derived from these animals. compared to wild ... | 1999 | 10616903 |
| new method for automatic identification identification and typing of single and multiple superimposed human papillomavirus sequences. | this study of specimens of human papillomaviruses (hpv) through hpv-specific polymerase chain reaction (pcr), followed by direct sequencing, resulted in 11% (38/354) superimposed hpv sequences, signifying coinfection with more than one hpv type. to address the diagnostic problem that these superimposed ("degenerated," overlapping) sequences pose, the authors created a papillomavirus database in microsoft excel (microsoft corporation, redmond, wa, u.s.a.) and corel quattro pro 9 (corel corporatio ... | 1999 | 10617279 |
| the human papillomavirus (hpv) 16 e2 protein induces apoptosis in the absence of other hpv proteins and via a p53-dependent pathway. | the human papillomavirus (hpv) e2 protein regulates viral gene expression and is also required for viral replication. hpv-transformed cells often contain chromosomally integrated copies of the hpv genome in which the viral e2 gene is disrupted. we have shown previously that re-expression of the hpv 16 e2 protein in hpv 16-transformed cells results in cell death via apoptosis. here we show that the hpv 16 e2 protein can induce apoptosis in both hpv-transformed and non-hpv-transformed cell lines. ... | 2000 | 10617590 |
| molecular cloning and nucleotide sequence analysis of a novel human papillomavirus (type 82) associated with vaginal intraepithelial neoplasia. | the genome of a novel human papillomavirus (hpv-82) was cloned from a vaginal intraepithelial neoplasia grade i. in our series of 291 biopsy specimens, hpv-82 was identified in one case each of cervical intraepithelial neoplasia grade ii and grade iii by blot hybridization. the histological localization of hpv-82 dna in the three lesions was confirmed by in situ hybridization. the results indicated that hpv-82 is an etiologic agent for vaginal and cervical intraepithelial neoplasia. by nucleotid ... | 2000 | 10618284 |
| ultrastructural study of intranuclear inclusion bodies of pulmonary adenocarcinoma. | intranuclear inclusion bodies are sometimes observed in pulmonary adenocarcinoma by light microscopy. electron microscopic characteristics of lung cancer cells with intranuclear inclusion bodies were studied. in addition, polymerase chain reaction (pcr) was performed using primers coding for human papillomavirus (hpv) types 16, 18, and 33. eosinophilic intranuclear inclusion bodies were observed in 22 out of 285 cases by light microscopy. immunohistochemically, cancer cell nuclei stained with pe ... | 1999 | 10626688 |
| genital infection with human papillomavirus (hpv) | 1999 | 10627183 | |
| [experimental investigations and clinical use of photodynamic therapy (pdt) in the rudolf foundation hospital]. | this article addresses experimental investigations and the clinical use of pdt in the rudolfstiftung hospital, vienna. we investigated mesotetrahydroxyphenylchlorine (mthpc) and the photosensitizer hematoporphyrin derivative alone or in combination to prove photodynamic antibacterial effects on staphylococcus aureus (wild type). mthpc showed antibacterial toxicity in the dark; hematoporphyrin derivative showed suppressive growth effects only after white-light illumination. photodynamic activity ... | 1999 | 10629388 |
| hpv16 e6 oncogene variants in women with cervical intraepithelial neoplasia. | human papillomaviruses (hpvs) are strongly associated with the development of high grade cervical intraepithelial neoplasia (cin) and cervical carcinoma, with between 40-80% of patients with cervical carcinoma being attributed to a single hpv type, hpv16 depending on the methods used and geographical location of the particular study [van den brule et al., 1996]. an hpv16 e6 variant has been described which is strongly associated with high grade cin [ellis et al., 1997] and with the human leukocy ... | 2000 | 10630967 |
| hpv dna testing of self-collected vaginal samples compared with cytologic screening to detect cervical cancer. | more than half of the women diagnosed as having cervical cancer in the united states have not been screened within the last 3 years, despite many having had contact with the health care system. in many other regions of the world, there is only limited access to cervical cancer screening. | 2000 | 10632284 |
| [antibody toward human papillomavirus type 16 (hpv16) l1-capsids]. | 1999 | 10635835 | |
| induction of cell-cycle arrest in cervical cancer cells by the human immunodeficiency virus type 1 viral protein r. | to determine the ability of the human immunodeficiency virus type 1 (hiv-1) gene vpr to induce cell-cycle arrest in cervical cancer cells with or without human papillomavirus (hpv) type 16 e6 or e7 expression. | 2000 | 10636517 |
| gene gun-mediated dna vaccination induces antitumor immunity against human papillomavirus type 16 e7-expressing murine tumor metastases in the liver and lungs. | dna vaccination has emerged as an attractive approach for tumor immunotherapy. the aim of this study was to evaluate the potency of dna vaccines in preventing and treating the liver and lung metastases of a human papillomavirus-16 (hpv-16) e7-expressing murine tumor (tc-1). we used the gene gun method to vaccinate c57bl/6 mice intradermally with dna vaccines containing the hpv-16 e7 gene, the e7 gene linked to the sorting signals of the lysosome-associated membrane protein-1 (sig/e7/ lamp-1), or ... | 1999 | 10637448 |
| [critical review of colpo-histological results in cervix pathology]. | the objective of this paper was to evaluate the role of squamous metaplasia in the determination of certain colposcopic appearances. | 1999 | 10638161 |
| immunogenicity of the b monomer of escherichia coli heat-labile toxin expressed on the surface of streptococcus gordonii. | the b monomer of the escherichia coli heat-labile toxin (ltb) was expressed on the surface of the human oral commensal bacterium streptococcus gordonii. recombinant bacteria expressing ltb were used to immunize balb/c mice subcutaneously and intragastrically. the ltb monomer expressed on the streptococcal surface proved to be highly immunogenic, as ltb-specific immunoglobulin g (igg) serum titers of 140,000 were induced after systemic immunization. most significantly, these antibodies were capab ... | 2000 | 10639444 |
| detection and typing of human papillomavirus dna by pcr using consensus primers in various cervical lesions of korean women. | the association between cervical cancers and human papillomavirus (hpv) is now well established. to estimate the extent of infection with common hpvs among korean women, we have examined 224 cervical scrapes of various cervical lesions. detection and typing of hpvs were done by polymerase chain reaction (pcr) using consensus primers followed by restriction enzyme digestion and pcr using type-specific primers. the prevalence of total hpv infection in patients with cervical intraepithelial neoplas ... | 1999 | 10642935 |
| capture elisa and in vitro cell binding assay for the detection of antibodies to human papillomavirus type 6b virus-like particles in patients with anogenital warts. | to investigate human papillomavirus (hpv) virus-like particle (vlp)-specific antibody responses among anogenital warts patients, a vlp-based capture elisa was established. twenty-six percent (35/134) of control subjects and 50.0% (39/78) of patients with current anogenital warts showed igg seropositivity to hpv 6b vlps. hpv 6b vlp-specific antibody responses recognised native vlps only, and had no cross-reaction with hpv type 16 vlps. no differences in reactivity were observed between l1 and l1 ... | 1999 | 10643018 |
| expression of human papillomavirus e7 mrna in human oral and cervical neoplasia and cell lines. | human papillomaviruses (hpvs) have been strongly linked to progression of human cancers, such as cervical and oral cancers. two hpv oncoproteins, e6 and e7, can inhibit the tumor suppressor proteins, p53 and prb, respectively, resulting in a deregulation of the cell cycle. in order to further test the significance of hpv expression in oral and cervical carcinogenesis, we analyzed hpv e7 mrna in oral and cervical neoplasia and cell lines by reverse transcriptase-polymerase chain reaction (rt-pcr) ... | 1999 | 10645408 |
| p53 alterations in recurrent squamous cell cancer of the head and neck refractory to radiotherapy. | the aim of the study was to determine the incidence of p53 alterations by mutation, deletion or inactivation by mdm2 or human papillomavirus (hpv) infection in recurrent squamous cell cancer of the head and neck (scchn) refractory to radiotherapy. twenty-two tumours were studied. the p53 status of each tumour was analysed by sequencing of exons 4-9 and by immunohistochemistry. mdm2 expression was assessed by immunohistochemistry and hpv infection was assessed by polymerase chain reaction of tumo ... | 2000 | 10646894 |
| correlation between human papillomavirus-associated cervical cancer and p53 codon 72 arginine/proline polymorphism. | high-risk mucosal human papillomaviruses encode an e6 oncoprotein, which binds the cellular p53 tumor suppressor protein, thereby marking it for degradation through the ubiquitin-mediated pathway. a common p53 polymorphism at codon-72 of exon 4 results in translation to either arginine or proline. recently reported data suggested an increased susceptibility to e6/ubiquitin-mediated degradation of the arg72-p53 isoform and an over-representation of the homozygous arg72-p53 genotype in cervical ca ... | 1999 | 10647890 |
| purification of his-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. | recombinant proteins overexpressed in and purified from escherichia coli contain impurities that are toxic in biological assays. the application of affinity purification procedures is often not sufficient to remove these toxic components. we here describe a simple and fast, one-step protocol to remove these impurities highly efficiently. four recombinant proteins were overexpressed in e. coli as his-tagged fusion proteins and purified by immobilized metal chelate affinity chromatography on ni-nt ... | 2000 | 10648174 |
| antibodies against oncoproteins e6 and e7 of human papillomavirus types 16 and 18 in cervical-carcinoma patients from russia. | certain human papillomaviruses (hpv), mainly types 16 and 18, have been widely recognized as an essential etiologic factor for the development of carcinoma of the uterine cervix. the early hpv proteins e6 and e7 are consistently expressed in the tumor cells, and cervical-carcinoma patients can develop antibodies against these oncoproteins. for cervical-carcinoma patients from eastern europe and russia, detailed information on hpv dna prevalence and hpv-specific immune responses is limited. the p ... | 2000 | 10652419 |
| a novel and rapid pcr-based method for genotyping human papillomaviruses in clinical samples. | many human papillomavirus (hpv) genotypes are associated with cervical carcinoma. we demonstrate the utility of an innovative technique for genotyping of hpv in cervical tissue samples. this method provides an accurate means of identification of the specific hpv genotypes present in clinical specimens. by using the my09-my11 and the gp5(+)-gp6(+) consensus primer pairs, hpv sequences were amplified by nested pcr from dna isolated from cervical smear samples. this led to the production of an appr ... | 2000 | 10655368 |
| laryngeal cancer and human papillomavirus: hpv is absent in the majority of laryngeal carcinomas. | thirty laryngeal carcinomas from patients without pre-existing laryngeal papillomatosis were examined by pcr for the presence of hpv dna. the utmost care was taken during sectioning of the tissue blocks and dna-extraction in order to avoid false positive results. three pairs of consensus primers were used: my9/my11, gp5+/gp6+ and cpi/cpii. hpv was detected in 1/30 carcinomas. the hpv type present could not be determined, but it was not type 6, 11, 13, 16, 18, 30, 31, 33, 35 or 45. in other studi ... | 1999 | 10656604 |
| human papillomavirus types 16 e6 and e7 contribute differently to carcinogenesis. | high-risk human papillomaviruses (hpvs) are etiologically implicated in human cervical cancer. two viral genes, e6 and e7, are commonly found expressed in these cancer cells. we have previously shown that mice transgenic for the hpv-16 e6 gene or e7 gene, in which the e6 or e7 was expressed in the basal layer of epithelia, developed skin tumors. the spectrum of tumors derived from e6 and e7 mice differed, however; although most tumors derived from the e7-transgenic mice were benign, the majority ... | 2000 | 10662610 |
| prevalence of and risks for cervical human papillomavirus infection and squamous intraepithelial lesions in adolescent girls: impact of infection with human immunodeficiency virus. | data suggest that in adults, human papillomavirus (hpv) infections and their sequalae, squamous intraepithelial lesions (sils), occur more commonly among human immunodeficiency (hiv)-infected women because of the hiv-associated cd4+ t-cell immunosuppression. since adolescents are more likely to be early in the course of hiv and hpv infections, the study of both infections in this age group may help elucidate their initial relationship. | 2000 | 10665598 |
| use of fluorogenic histocompatibility leukocyte antigen-a*0201/hpv 16 e7 peptide complexes to isolate rare human cytotoxic t-lymphocyte-recognizing endogenous human papillomavirus antigens. | cervical cancer (cacx) is the second most common female malignancy worldwide and remains a clinical problem despite improvements in early detection and therapy. cacx and preinvasive cervical intraepithelial neoplasia (cin3) are strongly associated with infection by human papillomavirus (hpv), particularly types 16 and 18. two nonstructural viral proteins, e6 and e7, are constitutively expressed in cervical tumors and are crucial for the maintenance of the transformed phenotype. these proteins th ... | 2000 | 10667589 |
| vulvar neoplasia and search for human papillomavirus 16 and 18 genetic information. short communication. | the presence of hpv 16 and 18 is frequent in cases with vulvar carcinomas and intraepithelial neoplasias. | 1999 | 10668162 |
| human papillomaviruses and dna ploidy in anal condylomata acuminata. | previous studies have emphasized the usefulness of dna ploidy measurement and human papillomavirus (hpv) detection as prognostic markers in low grade cervical lesions. we addressed the eventual relationship between hpv type, dna profile, and p53 tumor suppressor protein expression in anal condylomata acuminata to eventually determine parameters which may be considered as predictive risk factors for the development of cancer. dna ploidy was assessed by image cytometry after feulgen staining of co ... | 2000 | 10668198 |
| restored expression of fragile histidine triad protein and tumorigenicity of cervical carcinoma cells. | allelic losses in the short arm of chromosome 3 are common in cervical carcinomas. the fragile histidine triad (fhit) gene at chromosome region 3p14.2 is a candidate tumor suppressor gene that may play a role in cervical tumorigenesis. we and others have identified aberrant fhit transcripts and frequent loss of fhit protein expression in primary cervical cancers and high-grade noninvasive lesions but not in normal cervical tissues. the altered expression of fhit may be due to somatic mutations o ... | 2000 | 10675384 |
| both hpv and carcinogen contribute to the development of resistance to apoptosis during oral carcinogenesis. | oral carcinomas frequently contain human papilloma virus (hpv)-16/18. as p53 is degraded through interaction with hpv-16/18 products (e6/e7), p53 dysfunction may contribute to oral carcinogenesis. furthermore, epidemiological studies suggest that smoking history may be critical for oral carcinogenesis. to delineate the involvement of hpv-16 infection and carcinogen in oral carcinogenesis, park et al have established a multistep oral carcinogenesis model. overexpression of p53 altered the express ... | 2000 | 10675494 |
| determinants of low-risk and high-risk cervical human papillomavirus infections in montreal university students. | previous studies have been inconsistent about the degree of sexual transmissibility of cervical human papillomavirus (hpv) infection. the authors hypothesize that risk factors for hpv infection vary according to hpv type. | 2000 | 10676974 |
| asymptomatic penile hpv infection: a prospective study. | the occurrence of human papillomavirus (hpv) among males was analysed with the polymerase chain reaction (pcr) method. penile brush samples were taken once from 147 males attending for a control or for hpv non-related reasons, and consecutive samples were collected from 88 males re-attending the clinic. of the males attending once, 13% (19/147) were hpv dna positive and among the re-attenders 14% (12/88) were initially positive as compared with 33% (29/88) who were positive at least at one visit ... | 2000 | 10678474 |
| [detection of human papillomavirus infection in cervical pap smears by computer-assisted cytologic test]. | the sensitivity and accuracy of cytologic computer-assisted test (cct) in diagnosis of human papillomavirus (hpv) infection in cervical pap smear were evaluated. | 1998 | 10682433 |
| [the evaluation of computer cytological test with colposcopy for the diagnosis of cervical lesions]. | to assess the value of computer cytological test (cct) with colposcopy for the diagnosis of early cervical diseases. | 1998 | 10682434 |
| nuclear matrix attachment regions of human papillomavirus type 16 repress or activate the e6 promoter, depending on the physical state of the viral dna. | two nuclear matrix attachment regions (mars) bracket a 550-bp segment of the long control region (lcr) containing the epithelial cell-specific enhancer and the e6 promoter of human papillomavirus type 16 (hpv-16). one of these mars is located in the 5' third of the lcr (5'-lcr-mar); the other lies within the e6 gene (e6-mar). to study their function, we linked these mars in various natural or artificial permutations to a chimeric gene consisting of the hpv-16 enhancer-promoter segment and a repo ... | 2000 | 10684263 |
| recombinant adeno-associated virus expressing human papillomavirus type 16 e7 peptide dna fused with heat shock protein dna as a potential vaccine for cervical cancer. | in this study, we explore a potential vaccine for human papillomavirus (hpv)-induced tumors, using heat shock protein as an adjuvant, a peptide vaccine for safety, and adeno-associated virus (aav) as a gene delivery vector. the tumor vaccine was devised by constructing a chimeric gene which contained hpv type 16 e7 cytotoxic t-lymphocyte (ctl) epitope dna (m. c. feltkamp, h. l. smits, m. p. vierboom, r. p. minnaar, b. m. de jongh, j. w. drijfhout, j. ter schegget, c. j. melief, and w. m. kast, e ... | 2000 | 10684306 |
| hela cells are phenotypically limiting in cyclin e/cdk2 for efficient human papillomavirus dna replication. | human papillomaviral (hpv) origin-containing plasmids replicate efficiently in human 293 cells or cell extracts in the presence of hpv origin-recognition protein e2 and replication initiation protein e1, whereas cervical carcinoma-derived, hpv-18-positive hela cells or cell extracts support hpv dna replication poorly. we recently showed that hpv-11 e1 interacts with cyclin/cyclin-dependent kinase (cdk) complexes through an rxl motif and is a substrate for these kinases. e1 mutations in this moti ... | 2000 | 10692408 |
| interleukin 1 receptor antagonist gene polymorphism in women with vulvar vestibulitis. | vulvar vestibulitis is a chronic inflammatory syndrome of unknown cause and pathogenesis. we examined the relation between vulvar vestibulitis and polymorphisms in the gene coding for the interleukin 1 receptor antagonist, a naturally occurring down-regulator of proinflammatory immune responses. | 2000 | 10694325 |
| value of human papillomavirus testing. | 2000 | 10694358 | |
| transforming growth factor beta 1 (tgf beta 1) down-regulates expression and function of proliferation-inducing molecules in hpv-transformed cells. | the epidemiologic association of human papillomavirus (hpv) infection with dysplasia and cervical cancer is well established. transforming growth factor beta 1 (tgf beta 1) is a growth inhibitory protein for epithelial cells. to examine the phenotype of hpv-transformed cells, we examined expression of tgf beta 1 and a number of cellular proliferation-enhancing molecules which are known to be regulated by tgf beta 1, including bcl-2, c-jun and nfkb. previous studies had identified significant ind ... | 1999 | 10697499 |
| memorandum for: science writers and editors on the journal press list: limited potential for human papillomavirus (hpv) testing for women with low-grade pap smears | 2000 | 10699059 | |
| inactivation of interferon regulatory factor-1 tumor suppressor protein by hpv e7 oncoprotein. implication for the e7-mediated immune evasion mechanism in cervical carcinogenesis. | in studying biological roles of interferon regulatory factor (irf)-1 tumor suppressor in cervical carcinogenesis, we found that hpv e7 is functionally associated with irf-1. binding assays indicate a physical interaction between irf-1 and hpv e7 in vivo and in vitro. the carboxyl-terminal transactivation domain of irf-1 was required for the interaction. transient co-expression of e7 significantly inhibits the irf-1-mediated activation of ifn-beta promoter in nih-3t3 cells. co-transfection of e7 ... | 2000 | 10702232 |
| synthesis of viral dna and late capsid protein l1 in parabasal spinous cell layers of naturally occurring benign warts infected with human papillomavirus type 1. | we investigated human papillomavirus type 1 (hpv1)-specific transcription, viral dna replication, and viral protein expression in naturally occurring benign tumors by in situ hybridization, 5-bromodeoxyuridine (brdu) incorporation, and immunohistochemistry and obtained results different from other hpv-infected benign tumors characterized so far. moderate amounts of transcripts with a putative coding potential for e6/e7, e1, and e2 were demonstrated from the first subrabasal cell layer throughout ... | 2000 | 10704337 |
| enhancement of dna vaccine potency by linkage of antigen gene to an hsp70 gene. | nucleic acid vaccines represent an attractive approach to generating antigen-specific immunity because of their stability and simplicity of delivery. however, there is still a need to increase the potency of dna vaccines. using human papillomavirus type 16 e7 as a model antigen, we evaluated the effect of linkage to mycobacterium tuberculosis heat shock protein 70 (hsp70) on the potency of antigen-specific immunity generated by naked dna vaccines. we found that vaccines containing e7-hsp70 fusio ... | 2000 | 10706121 |
| knowledge of human papillomavirus infection among young adult men and women: implications for health education and research. | human papillomavirus (hpv) infection of the genital tract is one of the most common sexually transmitted diseases (stds), and a subset of genital tract hpvs are etiologically associated with cervical cancer. the prevalence of hpv infection is highest among adolescents and young adults. this study was undertaken to explore first year college students' knowledge about hpvs and to determine whether there were gender differences in this knowledge. an anonymous survey was distributed to all first yea ... | 2000 | 10706210 |
| combined interferon-gamma and tumor necrosis factor-alpha treatment differentially affects adhesion and migration of keratinocyte-derived cells to laminin-1. | interactions with the extracellular matrix constitute basic steps in cervix carcinoma cell invasion. in this study, we examined the adhesion and migration profiles of two human papillomavirus (hpv) dna-transfected keratinocyte-derived cell lines, eil8 and 18-11s3, and of the cervix adenocarcinoma siha cell line, towards laminin-1, and the selective effect of a 24-72 h treatment of 1000 u/ml interferon-gamma (ifn-gamma) and tumor necrosis factor-alpha (tnf-alpha), a treatment that significantly d ... | 2000 | 10714393 |
| population-based study of human papillomavirus infection and cervical neoplasia in rural costa rica. | human papillomavirus (hpv) is the main cause of cervical neoplasia. because few population-based studies have investigated the prevalence of type-specific infection in relation to cervical disease, we studied a high-risk population, estimating the prevalence of hpv infection and the risk associated with various hpv types. | 2000 | 10716964 |
| [detection of human papillomavirus in cutaneous extragenital bowen's disease in immunocompetent patients]. | a specific link between human papillomavirus (hpv) types 16, 18, 31, and 33 and genital carcinomas and between hpv type 5 and cutaneous extragenital carcinomas in patients with epidermodysplasia verruciformis and renal transplant has been previously found. the aim of this prospective study was to detect hpv in cases of cutaneous extragenital bowen's disease (bd) from non-immunosuppressed patients. | 2000 | 10717561 |
| cytokine profile in genital tract secretions from female adolescents: impact of human immunodeficiency virus, human papillomavirus, and other sexually transmitted pathogens. | quantitative enzyme-linked immunosorbent assays were used to measure interleukin (il)-2, il-10, and il-12 in cervical secretions from female adolescents with and without sexually transmitted infections. compared with human immunodeficiency virus [hiv]-negative patients, hiv-positive patients had higher concentrations of il-10 (118.2 pg/ml vs. 34.5 pg/ml; p=.002) and il-12 (175.5 pg/ml vs. 85.1; p=.03). il-2 concentrations were not statistically different. furthermore, genital tract infections we ... | 2000 | 10720516 |
| development, characterization and distribution of adoptively transferred peripheral blood lymphocytes primed by human papillomavirus 18 e7--pulsed autologous dendritic cells in a patient with metastatic adenocarcinoma of the uterine cervix. | we describe a 27-year-old woman with systemic chemoresistant and radioresistant metastatic disease secondary to a recurrence of human papillomavirus (hpv) 18 infected cervical adenocarcinoma of the uterine cervix who received adoptive transfer of peripheral blood t cells stimulated with hpv 18 e7-pulsed autologous dendritic cells (dc). extensive in vitro characterization of the dc-activated t cells derived from peripheral blood mononuclear cells (pbmc) included phenotypic analysis, cytotoxicity ... | 2000 | 10726612 |