Publications

the stressful condition as a nutritionally dependent adaptive dichotomy.	the injured body manifests a cascade of cytokine-induced metabolic events aimed at developing defense mechanisms and tissue repair. rising concentrations of counterregulatory hormones work in concert with cytokines to generate overall insulin and insulin-like growth factor 1 (igf-1), postreceptor resistance and energy requirements grounded on lipid dependency. salient features are self-sustained hypercortisolemia persisting as long as cytokines are oversecreted and down-regulation of the hypotha ...	1999	10319365
pertechnetate scintigraphy in primary congenital hypothyroidism.	primary congenital hypothyroidism (pch) is currently detected effectively by heel-stick screening. when elevated thyrotropin (tsh) and/or decreased t4 are found in the blood of neonates, they are recalled, values are confirmed in venous blood and thyroxine replacement therapy (trt) is immediately instituted, thus cretinism or severe retardation is prevented. however, in a significant percentage of neonates with abnormal blood levels of t4 or tsh, the disorder is transient. to help determine the ...	1999	10319753
in vivo expression of thyroid transcription factor-1 rna and its relation to thyroid function and follicular heterogeneity: identification of follicular thyroglobulin as a feedback suppressor of thyroid transcription factor-1 rna levels and thyroglobulin synthesis.	we used in situ hybridization to evaluate thyroid transcription factor-1 (ttf-1) rna expression in individual follicles and related this to thyroglobulin (tg) synthesis in vivo, as estimated by immunohistochemical analysis. we studied the thyroids of wistar rats treated with thyroxine (t4) or propylthiouracil (ptu), each of which modulates tsh levels, but affects follicular function and tg accumulation in the follicular lumen very differently. we show that ttf-1 rna levels in vivo correlate dire ...	1999	10319936
salsalate and salicylate binding to and their displacement of thyroxine from thyroxine-binding globulin, transthyrin, and albumin.	salsalate and its metabolite salicylate are inhibitors of thyroid hormone binding to serum transport proteins and reduce serum total thyroxine (t4) and triiodothyronine (t3) in vivo. the present study is the first to examine and compare salsalate and salicylate binding to human thyroxine-binding globulin (tbg), transthyretin (ttr), albumin (alb), and whole human serum, and displacement by salsalate and salicylate of t4 from isolated tbg, ttr, alb. salsalate and salicylate binding were studied by ...	1999	10319941
type of lymph node involvement and survival in pathologic n1 stage iii non-small cell lung carcinoma.	survival of patients with stage ii non-small lung cancer by the 1986 classification depends on the type of lymph node involvement (by direct extension or by metastases in lobar or hilar lymph nodes). the influence of these types of lymph node involvement on survival was investigated in pathologic n1 stage iii patients.	1999	10320225
developmental regulation of hepatic ceruloplasmin mrna and serum activity by exogenous thyroxine and dexamethasone.	ceruloplasmin (cp) is a copper-dependent oxidase with roles that include the regulation of iron metabolism, participation in the acute-phase response to inflammation, and antioxidant systems. although developmental increases in hepatic cp gene expression and serum activity have been described, the molecular mechanisms that are responsible for this regulation are not fully understood. the studies described here explored the possible role of glucocorticoids and thyroxine (t4) in the early neonatal ...	1999	10320628
sublingual-swallow immunotherapy (slit) in patients with asthma due to house-dust mites: a double-blind, placebo-controlled study.	a double-blind, placebo-controlled study was carried out in 85 patients with a well-documented history of perennial asthma caused by house-dust mites. patients received either placebo or sublingual immunotherapy (slit) with a standardized dermatophagoides pteronyssinus (dp)-d. farinae (df) 50/50 extract. after a run-in period, patients received increasing doses up to 300 ir every day for 4 weeks and then three times a week for the following 24 months. the cumulative dose was about 104000 ir, equ ...	1999	10321561
bacteriophage t4 rnh (rnase h) null mutations: effects on spontaneous mutation and epistatic interaction with rii mutations.	the bacteriophage t4 rnh gene encodes t4 rnase h, a relative of a family of flap endonucleases. t4 rnh null mutations reduce burst sizes, increase sensitivity to dna damage, and increase the frequency of acriflavin resistance (acr) mutations. because mutations in the related saccharomyces cerevisiae rad27 gene display a remarkable duplication mutator phenotype, we further explored the impact of rnh mutations upon the mutation process. we observed that most acr mutants in an rnh+ strain contain a ...	1999	10322013
[verification and epidemiological study on endemic iodide-induced goiter in jiangsu old yellow river flooded plain].	to verify the existence and reveal the epidemiological characteristics of endemic iodide-induced goiter in jiangsu old yellow river flooded plain, 12 spots were selected in this survey. the results showed that the total goiter rate of 22.18% by palpation and 12.83% by b-ultrasound which surpassed the 5%-cutpoint-recommended jointly by who/unicef/iccidd. the urinary iodine median was 962 micrograms/l in 607 samples. the average 24 hours i31iodine-emissivity ratio was 4.6 +/- 0.3% with most of the ...	1998	10322720
[effect of yifei kangliu yin on lung cancer metastasis and immune function].	to explore the effect of yifei kangliu yin (yfkly) on metastasis of lung cancer and immune function.	1997	10322858
expression of preproopiomelanocortin mrna and preprodynorphin mrna in brain of spontaneously hypertensive rats.	to compare the expressions of prepropiomelanocortin (pomc) mrna and preprodynorphin (ppd) mrna between 16-wk-old spontaneously hypertensive rats (shr) and age-matched normotensive wistar-kyoto rats (wky).	1997	10322923
effects of dopexamine on blood flow in multiple splanchnic sites measured by laser doppler velocimetry in rabbits undergoing cardiopulmonary bypass.	decreased gut perfusion has been reported during cardiopulmonary bypass (cpb). studies of treatments to avoid splanchnic ischaemia during cpb have given conflicting results. we studied 12 rabbits during mild hypothermic non-pulsatile cpb. tissue blood flow in three different splanchnic areas (gastric, jejunum and ileum) was measured by laser doppler velocimetry (ldv) before cpb (t0), after steady state (t1), after administration of dopexamine 2 micrograms kg-1 min-1 (t2) and 4 micrograms kg-1 mi ...	1999	10325845
a comparative study of computerised visual field testing and optic disc morphometric parameters in the follow-up of primary open angle glaucoma.	to evaluate the correlation between computerised visual field testing and optic disc morphometric parameters (rim area, rim/disc area ratio, cup/disc area ratio and optic disc surface smoothness (odss)) in the follow-up of a group of patients affected by primary open angle glaucoma (poag).	1998	10325985
[spinal localization of gorham's syndrome. case report].	the authors report a case of massive osteolysis of the spine in an eighteen years old boy presenting a t9 collapse without neurological symptoms associated to a right chylothorax which disappeared after 5 days of drainage. mri showed an increased signal on t1 and t2 weighted sequences. an orthopaedic treatment with a cast was decided as a first attempt. because on increasing pain and kyphosis, surgery was considered, with posterior segmental fixation with hartshill rectangle from t4 to l5. the r ...	1999	10327471
thyroid hormones in grey seal pups (halichoerus grypus).	the aim of the present study was to describe the changes in thyroid hormone status in grey seal (halichoerus grypus) pups from birth to weaning and moulting. plasma concentrations of total thyroxine (tt4) were highest the first two days after birth, thereafter dropping to a lower, but stable level. this pattern may reflect a high transfer rate of maternal thyroxine prepartum, prior to parturition, or postpartum via colostrum, or it may be caused by active secretory thyrocytes in late foetal stag ...	1999	10327615
post-transcriptional control of bacteriophage t4 gene 25 expression: mrna secondary structure that enhances translational initiation.	secondary structure of the mrna in the translational initiation region is an important determinant of translation efficiency. however, the secondary structures that enhance or facilitate translation initiation are rare. we have previously proposed that such structure may exist in the case of bacteriophage t4 gene 25 translational initiation region, which contains three potential shine-dalgarno sequences (sd1, sd2, and sd3) with a spacing of 8, 17, and 27 nucleotides from the initiation codon of ...	1999	10329143
acute effects of thyroid hormone analogs on sodium currents in neonatal rat myocytes.	we previously reported that t3(3,3',5-triiodo-l-thyronine) acutely increases sodium currents (ina) in neonatal rat myocytes. here we compare the effects of several thyroid hormone analogs, including t4(3,3',5,5'-tetraiodo-l-thyronine), rt3(3,3',5'-triiodo-l-thyronine), d-t3(3,3',5-triiodo-d-thyronine), 3,5-t2(3,5-diiodo-l-thyronine), dit (3,5-diiodo-l-tyrosine), mit (3-monoiodo-l-tyrosine), tetrac (3,3',5,5'-tetraiodo-thyroacetic acid), triac (3, 3',5-triiodo-thyroacetic acid), and tyrosine, on ...	1999	10329215
establishment of a new system for determination of coreceptor usages of hiv based on the human glioma np-2 cell line.	cd4 and one of the g-protein-coupled receptors (gpcrs) on the cell surface function as a receptor and a coreceptor, respectively, in infection of cells with human and simian immunodeficiency viruses (hiv/siv). to determine which gpcrs can be coreceptors for hiv (hiv-1 and hiv-2) or siv infection, several cell lines, including human osteosarcoma hos-t4 cells and human glioma u87/cd4 cells, have been used. however, these cells often show susceptibilities to some hiv or siv strains before transduct ...	1999	10329384
mutations which alter the elbow region of trna2gly reduce t4 gene 60 translational bypassing efficiency.	translating ribosomes bypass a 50 nucleotide coding gap in bacteriophage t4 gene 60 mrna between codons 46 and 47 in order to synthesize the full-length protein. bypassing of the coding gap requires peptidyl-trna2gly detachment from a gga codon (codon 46) followed by re-pairing at a matching gga codon just before codon 47. using negative selection, based on the sacb gene from bacillus subtilis, escherichia coli mutants were isolated which reduce bypassing efficiency. all of the mutations are in ...	1999	10329634
thyroid hormone induces activation of mitogen-activated protein kinase in cultured cells.	thyroid hormone [l-thyroxine (t4)] rapidly induced phosphorylation and nuclear translocation (activation) of mitogen-activated protein kinase (mapk) in hela and cv-1 cells in the absence of cytokine or growth factor. a pertussis toxin-sensitive and guanosine 5'-o-(3-thiotriphosphate)-sensitive cell surface mechanism responsive to t4 and agarose-t4, suggesting a g protein-coupled receptor, was implicated. cells depleted of mapk or treated with mapk pathway inhibitors showed reduced activation of ...	1999	10329948
hypothyroidism in elderly patients.	hypothyroidism is an endocrine condition found in elderly patients that is often confused with normal changes associated with aging. consequently, the disorder may be quite advanced before it is diagnosed and treated. depressed t4 and elevated tsh levels confirm primary hypothyroidism. patients are required to take levothyroxine for the rest of their lives. myxedema coma, a life-threatening complication of hypothyroidism, occurs primarily in elderly women and requires immediate treatment to prev ...	1999	10332556
induction chemotherapy and radiation therapy for t4 oropharyngeal carcinoma.	between 1964 and 1996, 123 patients were treated for t4 oropharyngeal carcinoma; 93 were treated with radiation therapy alone; 30 were treated with induction chemotherapy and radiation therapy. patients who received induction chemotherapy and radiation therapy were treated between 1985 and 1996; during this time 39 patients were treated with radiation therapy alone. five-year local control rates for patients undergoing chemotherapy and radiation therapy, radiation therapy alone (all patients), a ...	1999	10333250
high ligation of the inferior mesenteric artery with hypogastric nerve preservation in rectal cancer surgery.	we describe herein a technique of performing upward node dissection following high ligation of the inferior mesenteric artery for patients with t3 and t4 rectal carcinomas. the course of the hypogastric nerve is confirmed macroscopically during the procedure to ensure its preservation. this technique offers both increased radicality and the prevention of ejaculatory dysfunction.	1999	10333426
[the influence of salmon calcitonin on changes in thyroid hormone concentration and on tsh secretion. i].	the aim of this investigation was determination of salmon calcitonin (sct) influence on changes of thyroid hormones levels in serum. the investigation was carried out on a group of 26 patients. the group was divided in two different ways: into people younger and older than 65 years, and into men and women. investigation protocol consists of 4 days. on the 1st day patients received 100u of sc, on the 2nd day--100u of sc and 0.4 mg trh, on the 3rd day--0.4 mg trh, on the 4th day--0.9% solution of ...	1998	10335024
interaction of tamoxifen with concurrent cytotoxic adjuvant treatment affects lymphocytes and lymphocyte subsets counts in breast cancer patients.	the effects and interaction of endocrine and cytotoxic adjuvant treatment on measures of cellular immunity were assessed in 41 stage i-ii breast cancer patients from international breast cancer study group trials. counts of lymphocytes and lymphocyte subsets [(t, t4, t8, b, natural killer (nk) and activated t (at) cells] were assessed by flow cytometry immediately before adjuvant therapy at baseline and on day 1 of the 3rd cycle. twenty-two patients received cyclophosphamide, methotrexate and 5- ...	1999	10335933
the non-enzymatic microbicidal activity of lysozymes.	t4 lysozyme was thought to destroy bacteria by its muramidase activity. however, we demonstrate here that amphipathic helix stretches in the c-terminus of t4 lysozyme mediate its bactericidal and fungistatic activities. in heat-denatured t4 lysozyme, the enzymatic activity is completely abolished but unexpectedly, the antimicrobial functions remain preserved. small synthetic peptides corresponding to amphipathic c-terminal domains of t4 lysozyme show a microbicidal activity. its membrane disturb ...	1999	10338111
structural characterization of an engineered tandem repeat contrasts the importance of context and sequence in protein folding.	to test a different approach to understanding the relationship between the sequence of part of a protein and its conformation in the overall folded structure, the amino acid sequence corresponding to an alpha-helix of t4 lysozyme was duplicated in tandem. the presence of such a sequence repeat provides the protein with "choices" during folding. the mutant protein folds with almost wild-type stability, is active, and crystallizes in two different space groups, one isomorphous with wild type and t ...	1999	10339544
is it possible to diagnose canine hypothyroidism?	a definitive diagnosis of hypothyroidism can be difficult because of the many clinical abnormalities associated with thyroid hormone deficiency, and the lack of readily available diagnostic tests with high sensitivity and specificity. thyroid function tests should be performed only in dogs with clinical findings consistent with hypothyroidism. measurement of serum total thyroxine (t4) concentration is a useful initial screening test since most hypothyroid dogs have values below the reference ran ...	1999	10340243
hiv pathogenesis: gp120-antibody complexes bind cd4 and kill t4 cells--immunotoxin therapy should prevent the progression of hiv to aids.	current models of the role of human immunodeficiency virus (hiv) in the pathogenesis of acquired immune deficiency syndrome (aids) are inadequate and inconsistent with the literature. this article reviews a wide range of aids research and proposes the first model of hiv pathogenesis that is entirely consistent with the literature. this model is based on antibody-gp120 complex crosslinking of cd4 on the t4 cells. previously unexplained observations embraced by this model include early qualitative ...	1999	10340294
intraoperative pleural lavage in esophageal carcinoma.	cytological examination of intraoperative pleural or peritoneal lavage specimens is useful for predicting outcomes for patients with various carcinomas. there have been few reports regarding cytological examination of pleural lavage fluid in esophageal carcinoma.	1999	10340891
recombinant interferon alpha (rifn-alpha) does not potentiate the effect of iodine excess on the development of thyroid abnormalities in patients with hcv chronic active hepatitis.	to determine whether the administration of pharmacological quantities of iodine during interferon-alpha (rifn-alpha) treatment of chronic viral hepatitis b and c (hcv) would exacerbate the potential adverse effects of rifn alpha on thyroid function.	1999	10341861
kinetics of red blood cell t3 uptake in hypothyroidism with or without hormonal replacement, in the rat.	l-triiodothyronine (l-t3) is taken up and accumulated into red blood cells (rbc) by means of a specific carrier-mediated system. the aim of this study was to evaluate the reactivity of this system in relation to induced alterations in thyroid hormone (th) supply. we investigated the kinetic parameters (vmax, maximal velocity and km, michaelis constant) of washed-rbc l-t3 uptake 1) in thyroidectomized (txt) rats, 2) in txt rats administered with low doses of l-t4 (15 microg/kg/day x 14) to restor ...	1999	10342358
superior sulcus lung tumors: impact of local control on survival.	our goal was to assess patient survival and response to treatment for superior sulcus tumors treated with combined radiation therapy and surgery when possible, or with radiation alone when surgery was not possible.	1999	10343256
massive goitre (struma parenchymatosa) in geese.	in a goose flock consisting of 2300 birds of 6 months of age severe goitre was diagnosed. to the best of our knowledge this is the first report of naturally occurring goitre in geese, which is not related to the feeding of rapeseed meal. the major pathological findings included retarded growth and plumage development, significantly (300%) increased relative thyroid weight, fat accumulation in the mesenteric and abdominal region, and lipid infiltration of liver and kidney cells. subsequent hormon ...	1999	10344082
replication-induced protein synthesis and its importance to proteomics.	replication-induced protein synthesis (rips) can occur following the passage of the replisome due to transcription initiated by rna polymerase in association with: (i) negative supercoiling trailing the replisome / replication fork, (ii) hemimethylation prior to the action of dam methylase, (iii) transient derepression following passage of the replisome/replication fork and prior to renewed synthesis of the repressor gene-product, and (iv) 'sliding clamp' accessory dna-binding proteins binding t ...	1999	10344230
3-amino-5-phenoxythiophenes: syntheses and structure-function studies of a novel class of inhibitors of cellular l-triiodothyronine uptake.	a series of substituted 3-amino-5-phenoxythiophenes was synthesized starting from malodinitrile and carbon disulfide. the resulting dicyanoketenedithiolate reacts via thorpe-dieckmann cyclization with halogen methanes bearing electron-withdrawing groups to give thiophene-2-thiolates, which can be transformed into 3-amino-5-(methylsulfonyl)thiophene-4-carbonitriles. replacement of the methylsulfonyl groups by substituted phenolates provides the substituted 3-amino-5-phenoxythiophenes. some of the ...	1999	10346938
folding and stability of mutant scaffolding proteins defective in p22 capsid assembly.	bacteriophage p22 scaffolding subunits are elongated molecules that interact through their c termini with coat subunits to direct icosahedral capsid assembly. the soluble state of the subunit exhibits a partially folded intermediate during equilibrium unfolding experiments, whose c-terminal domain is unfolded (greene, b., and king, j. (1999) j. biol. chem. 274, 16135-16140). four mutant scaffolding proteins exhibiting temperature-sensitive defects in different stages of particle assembly were pu ...	1999	10347166
role of leptin in women with eating disorders.	this study investigated the role of leptin on eating behavior and reproductive function in eating disorders.	1999	10349581
nmr observation of t-tetrads in a parallel stranded dna quadruplex formed by saccharomyces cerevisiae telomere repeats.	we report here the nmr structure of the dna sequence d-tggtggc containing two repeats of saccharomyces cerevisiae telomere dna which is unique in that it has a single thymine in the repeat sequence and the number of gs can vary from one to three. the structure is a novel quadruplex incor-porating t-tetrads formed by symmetrical pairing of four ts via o4-h3 h-bonds in a plane. this is in contrast to the previous results on other telomeric sequences which contained more than one t in the repeat se ...	1999	10352174
analysis of the cooperative thermal unfolding of the td intron of bacteriophage t4.	the thermal stability of folded transcripts of the td intron of bacteriophage t4 that carried up to three base substitutions was investigated by temperature gradient gel electrophoresis (tgge) and uv melting. the unfolding of this autocatalytic group i intron is endothermic and entropically driven. although the effects of mutations in base pairs follow in most cases the expected order g-c>a-u>g.u>a.c, the extent of global destabilization varies strongly according to the helix in which substituti ...	1999	10352179
immunologic and biochemical factors in hyperemesis gravidarum with or without hyperthyroxinemia.	this study was set up to investigate the relationship between immune process and high levels of human chorionic gonadotropin-beta (betahcg) in hyperemesis patients with or without hyperthyroxinemia.	1999	10352382
[conservative surgical treatment in differentiated thyroid carcinoma].	purpose of the present study was to compare survival of patients affected by differentiated thyroid carcinoma after total and partial thyroidectomy.	1999	10352523
increased serum angiogenin concentration in colorectal cancer is correlated with cancer progression.	we have previously demonstrated that the increased expression of angiogenin (ang) in pancreatic cancer is related to cancer aggressiveness; however, the relationship between ang expression and its clinical relevance in colorectal cancer has not been demonstrated. we therefore investigated the correlation between serum ang (sang) concentration and colorectal cancer progression or the changes in sang concentrations before and after cancer resection. to determination sang concentration by elisa, se ...	1999	10353747
growth, puberty, and endocrine functions in patients with sporadic or familial neurofibromatosis type 1: a longitudinal study.	this study prospectively evaluates parameters of growth, puberty, and attained adult height in children with sporadic or familial occurrence of neurofibromatosis type 1 (nf-1), followed up longitudinally, to define the most important factors affecting these parameters.	1999	10353939
the 0.8% ultraviolet b content of an ultraviolet a sunlamp induces 75% of cyclobutane pyrimidine dimers in human keratinocytes in vitro.	tanning lamps, emitting predominantly ultraviolet (uv) a, are used widely throughout the u.k. and other countries, but little is known about the long-term risks associated with their use, especially with respect to skin cancer. we have exposed normal human epidermal keratinocytes to a commercial tanning lamp and used the comet assay in association with dna repair enzymes t4 endonuclease v and endonuclease iii to investigate the relative yields of directly formed cyclobutane pyrimidine dimers (cp ...	1999	10354066
a multifrequency electron spin resonance study of t4 lysozyme dynamics.	electron spin resonance (esr) spectroscopy at 250 ghz and 9 ghz is utilized to study the dynamics and local structural ordering of a nitroxide-labeled enzyme, t4 lysozyme (ec 3.2.1.17), in aqueous solution from 10 degrees c to 35 degrees c. two separate derivatives, labeled at sites 44 and 69, were analyzed. the 250-ghz esr spectra are well described by a microscopic ordering with macroscopic disordering (momd) model, which includes the influence of the tether connecting the probe to the protein ...	1999	10354455
combined sequential approach in locally advanced breast cancer.	the interaction between primary and adjuvant chemotherapy is a crucial point in the treatment of locally advanced breast cancer.	1999	10355574
strategies for recombinant protein recovery from canolaby precipitation	transgenic plants may prove to be one of the most economical systems for the large-scale production of proteins and peptides. our goal is to develop an approach for protein recovery from canola that will be adaptable to a wide variety of recombinant proteins. for recombinant protein recovery, the two downstream processes considered were extraction of target protein and purification of recombinant protein from host proteins and other impurities. in these experiments, t4 lysozyme has been added to ...	1999	10356267
aqueous two-phase systems containing urea: influence on phase separation and stabilization of protein conformation by phase components.	during recombinant escherichia coli fermentation with high expression levels, inclusion bodies are often formed. aqueous two-phase systems have been used in the presence of urea for the initial recovery steps. to investigate phase behavior of such systems we determined phase diagrams of poly(ethylene glycol) (peg)/sodium sulfate/urea/water and peg/dextran t-500 (dex)/urea/phosphate buffer/water at different concentrations of urea and different molecular weight of peg. peg/na2so4 aqueous two-phas ...	1999	10356268
enhancing or suppressive effects of antibodies on processing of a pathogenic t cell epitope in thyroglobulin.	thyroglobulin (tg)-specific abs occur commonly in thyroid disease, but it is not clear to what extent they affect tg processing and presentation to t cells. here we show that generation of the nondominant pathogenic tg epitope (2549-2560), containing thyroxine (t4) at position 2553 (t4(2553)), is augmented by tg-specific igg mabs that facilitate fcr-mediated internalization of tg. however, other mabs of the same (igg1) subclass enhanced tg uptake by apc but had no effect on the generation of thi ...	1999	10358139
unexpected abundance of self-splicing introns in the genome of bacteriophage twort: introns in multiple genes, a single gene with three introns, and exon skipping by group i ribozymes.	analysis of rna that can be labeled with gtp indicates the existence of group i introns in genes of at least three transcriptional classes in the genome of staphylococcus aureus bacteriophage twort. a single orf of 142 amino acids (orf142) is interrupted by three self-splicing group i introns, providing the first example of a phage gene with multiple intron insertions. twort orf142 is encoded in a message that is abundant 15-20 min after infection and is highly similar to a late gene product (or ...	1999	10359829
mutational analysis of coms: evidence for the interaction of coms and meca in the regulation of competence development in bacillus subtilis.	the development of bacillus subtilis genetic competence is a highly regulated adaptive response to stationary-phase stress. a key step in competence development is the activation of the transcriptional regulator comk, which is required for the expression of genes encoding the products that function in dna uptake. in log-phase cultures, comk is trapped in a complex composed of meca and clpc, in which it is rendered inactive. the coms gene, contained within the srf operon, is induced in response t ...	1999	10361283
effects of pyridostigmine and naloxone on the abnormal tsh response to trh during starvation in humans.	starvation is associated with a blunted tsh response to thyrotropin-releasing hormone (trh) (peak minus baseline < 5 miu/l), despite basal tsh and thyroid hormone levels within the normal range. in light of the inhibitory effect of somatostatin on tsh secretion, we examined whether this condition is caused by an increased hypothalamic somatostatinergic tone in starving subjects. the possible involvement of endogenous opioids in the mechanism underlying the abnormal tsh response to trh was also e ...	1999	10361382
bronchogenic carcinoma in young patients.	some investigators have suggested that lung cancer in young patients has a more aggressive course and poorer prognosis than lung cancer in older patients.	1999	10362088
quetiapine, a novel antipsychotic: experience in elderly patients with psychotic disorders. seroquel trial 48 study group.	this uncontrolled trial examines the safety and effects of quetiapine, a new atypical antipsychotic, in elderly patients with psychotic disorders.	1999	10362435
fold recognition study of alpha3-galactosyltransferase and molecular modeling of the nucleotide sugar-binding domain.	the structure and fold of the enzyme responsible for the biosynthesis of the xenotransplantation antigen, namely pig alpha3 galactosyltransferase, has been studied by means of computational methods. secondary structure predictions indicated that alpha3-galactosyltransferase and related protein family members, including blood group a and b transferases and forssman synthase, are likely to consist of alternating alpha-helices and beta-strands. fold recognition studies predicted that alpha3-galacto ...	1999	10362841
[hypothyroidism in patients treated for laryngeal cancer: preliminary results].	the reported incidence of hypothyroidism following surgery and/or irradiation for laryngeal cancer varies widely and the condition often is misdiagnosed. this study examines the incidence of thyroid dysfunction in patients with laryngeal cancer.	1999	10362864
vector analysis of visual evoked potentials elicited by pattern reversal and photic stimuli.	the 2d veps to pattern reversal (pr) and led goggle were studied in order to obtain a stable parameter for the functional assessing of posterior visual pathways regardless of the stimulus type used.	1999	10363760
effects of experimental hypothyroidism on 5-ht1a, 5-ht2a receptors, 5-ht uptake sites and tryptophan hydroxylase activity in mature rat brain1.	the study was aimed at investigating the repercussions of deficiency in thyroid function with and without thyroid hormone (th) replacement on the neurochemical entities which underly serotonin (5-ht) neutrotransmission, namely 5-ht1a, 5-ht2a receptors, 5-ht transporter and tryptophan hydroxylase (tph) in the mature brain. surgically thyroidectomized male wistar rats received: (1) an iodine-free diet to produce severe hypothyroidism; (2) hormonal replacement with 15 microgram/kg/day of thyroxine ...	1999	10364698
thyroid hormones regulate the expression of somatostatin receptor subtypes in the rat pituitary.	circulating tsh levels are increased in hypothyroidism and suppressed in hyperthyroidism. on the other hand, the hypothalamic hormone somatostatin suppresses basal and trh-induced tsh release, an effect which is enhanced by thyroid hormones. to investigate whether the effects of thyroid hormones on tsh secretion may be mediated in part through alterations in the gene expression of pituitary somatostatin receptors (sstr), 3-week-old male sprague-dawley rats were rendered hypothyroid with antithyr ...	1999	10364699
polychlorinated dibenzo-p-dioxins and related compounds: the blood levels of young japanese women.	we investigated pcdds and related compounds in the blood of young japanese women, approximately 20 years of age, who had not yet had children, and discussed how the teq level of pcdds and related compounds in their blood may affect the next generation. means of total teq levels were 0.063 pg/g for whole blood basis and 21 pg/g for lipid basis. teq of pcdds, pcdfs and coplanar pcbs accounted for about 43, 34 and 23% of the total teq in the whole blood basis, respectively. in the lipid basis, thei ...	1999	10365432
wavelength dependence of ultraviolet-induced dna damage distribution: involvement of direct or indirect mechanisms and possible artefacts.	dna damage profiles have been established in plasmid dna using purified dna repair enzymes and a plasmid relaxation assay, following exposure to uvc, uvb, uva or simulated sunlight (ssl). cyclobutane pyrimidine dimers (cpds) are revealed as t4 endonuclease v-sensitive sites, oxidation products at purine and pyrimidine as fpg- and nth-sensitive sites, and abasic sites are detected by nfo protein from escherichia coli. cpds are readily detected after uva exposure, though produced 10(3) and 10(5) t ...	1999	10365447
serum lipoproteins and apolipoprotein e in infants with congenital hypothyroidism.	hypothyroid adults have a high risk of atherosclerosis, secondary to increased levels of various cholesterol fractions, particularly low-density lipoprotein cholesterol (ldl-c). we investigated the existence of a correlation between thyroid hormone deficiency and serum lipoproteins and a possible effect of different apolipoprotein e (apoe) phenotypes on lipoprotein levels in 75 infants with hypothyroidism.	1999	10365572
subclinical hypothyroidism in a biethnic, urban community.	to evaluate the association between hypothyroidism, and the health status of older hispanic and non-hispanic white (nhw) men and women. to accomplish this, we determined the prevalences of the treated and untreated conditions and examined the associations between an elevated serum thyroid stimulating hormone (tsh) and cognitive and affective (mood) functions and the prevalences of symptoms and comorbidity, specifically coronary heart disease (chd), diabetes, hypertension, and hyperlipidemia.	1999	10366170
thyroid function, morphology and autoimmunity in young patients with insulin-dependent diabetes mellitus.	an association between insulin-dependent diabetes mellitus (iddm) and autoimmune thyroid disease is well recognized. we have studied the prevalence of thyroid dysfunction, autoimmunity and morphological abnormalities by ultrasonography in young diabetics.	1999	10366407
analysis of capsid portal protein and terminase functional domains: interaction sites required for dna packaging in bacteriophage t4.	bacteriophage dna packaging results from an atp-driven translocation of concatemeric dna into the prohead by the phage terminase complexed with the portal vertex dodecamer of the prohead. functional domains of the bacteriophage t4 terminase and portal gene 20 product (gp20) were determined by mutant analysis and sequence localization within the structural genes. interaction regions of the portal vertex and large terminase subunit (gp17) were determined by genetic (terminase-portal intergenic sup ...	1999	10366503
endoscopic ultrasound and fine needle aspiration for the evaluation of pancreatic masses.	endoscopic ultrasound (eus) and endoscopic ultrasound-guided fine needle aspiration (eus-fna) are accurate for the preoperative staging of pancreatic ductal carcinoma.	1999	10367874
endocrine effects, efficacy and tolerability of a 10.8-mg depot formulation of goserelin acetate administered every 13 weeks to patients with advanced prostate cancer.	to determine the endocrine effects, efficacy and tolerability of a 10.8-mg depot formulation of zoladextrade mark (goserelin acetate, zeneca pharmaceuticals, wilmington, delaware, usa), a luteinizing hormone-releasing hormone agonist analogue, when administration was extended from every 12 weeks to every 13 weeks in patients with advanced prostate cancer.	1999	10368200
inhibition of escherichia coli rna polymerase by bacteriophage t7 gene 2 protein.	the 64 amino acid residue product of bacteriophage t7 gene 2 (gp2) binds the escherichia coli rna polymerase and inhibits transcription. we localized the gp2 binding site to within 53 amino acid residues in the functionally dispensable region of the rna polymerase beta' subunit. we investigated the effect of gp2 on transcription at a -10/-35 promoter and at an "extended -10" promoter. our results indicate that binding of gp2 to the sigma70holoenzyme (esigma70) prevents promoter recognition at -1 ...	1999	10369763
pre-operative staging of recto-sigmoid colon carcinoma by upper gastrointestinal endoscopic ultrasonography.	to assess the accuracy of a new generation endoscopic ultrasonography (eus)(gf-m200) in pre-operative staging of recto-sigmoid colon carcinoma invasion and lymph node metastasis.	1999	10370633
ampullary carcinoma: prognostic significance of ploidy, cell-cycle analysis and proliferating cell nuclear antigen (pcna).	the aim of the present study is to assess the nuclear dna ploidy patterns, the fraction of cells in the various phases of the cell cycle as determined by flow cytometry and to evaluate proliferative cell-nuclear antigen (pcna) expression in order to examine the relationships between phase-two molecular factors, clinicopathological aspects and outcome of patients with cancers of the ampulla of vater.	1999	10370689
the drop in plasma thyrotropin concentrations in fasted chickens is caused by an action at the level of the hypothalamus: role of corticosterone.	fasting has severe effects on thyroid metabolism in the chicken: plasma thyroxine (t4) concentrations increase, whereas 3',5,3-triiodothyronine (t3) concentrations decrease. in the present report we studied the effect of fasting at the level of: 1) the pituitary (plasma thyrotropin (tsh) concentrations; the sensitivity of thyrotrophs to corticotropin-releasing hormone (crh) and tsh-releasing hormone (trh)); and 2) the hypothalamus (trh content). a regulatory role of corticosterone is discussed. ...	1999	10370862
both atrial resection and superior vena cava replacement in sleeve pneumonectomy for advanced lung cancer.	extended sleeve pneumonectomy including removal of the superior vena cava, right atrium and parts of left atrium on cardiopulmonary bypass was successfully performed in a 40-year-old man. the tumour was histologically proven a t4 n1 stage with margins free from tumour. adjuvant radiochemotherapy was administered postoperatively on an outpatient base. the patient did well for 7 months then he died from myocardial infarction due to metastatic infiltration of the right coronary artery. other metast ...	1999	10371135
evaluation of early reperfusion and i.v. tpa therapy using diffusion- and perfusion-weighted mri.	to characterize the effects of recombinant tissue plasminogen activator (rt-pa) therapy and early reperfusion on diffusion-weighted (dwi) and perfusion-weighted imaging (pwi) changes observed following acute ischemic injury.	1999	10371525
[our experience of carcinoma of the larynx in togo].	this is a retrospective study of 33 patients with squamous cell carcinoma of the larynx from the end ward at tokoin teaching hospital in lomé. this series is from 1981 and 1995 (1 t1, 6 t2, 15 t3, 11 t4). this is an uncommon cancer. no etiological factors were identified. the high proportion requiring emergency tracheotomy (36.36%) and of t3-t4 cases (78.78%) are explained by the long delay before the first medical visit. only 13 patients (36.36%) had a total laryngectomy. the prognosis was unfa ...	1999	10371859
left ventricular diastolic dysfunction in patients with subclinical hypothyroidism.	although subclinical hypothyroidism is frequently diagnosed, the decision to institute a substitutive therapy with l-t4 remains controversial. because the cardiovascular system is considered a main target for the action of thyroid hormone, we investigated whether subclinical hypothyroidism induces cardiovascular abnormalities. twenty-six patients (mean age, 36 +/- 12 yr) were evaluated by doppler-echocardiography, whereas a subgroup of 10 patients, randomly selected, were reevaluated after 6 mon ...	1999	10372711
functional and physical interaction between wrn helicase and human replication protein a.	the human premature aging disorder werner syndrome (ws) is associated with a large number of symptoms displayed in normal aging. the wrn gene product, a dna helicase, has been previously shown to unwind short dna duplexes (</=53 base pairs) in a reaction stimulated by single-stranded dna-binding proteins. we have studied the helicase activity of purified wrn protein on a variety of dna duplex substrates to characterize the unwinding properties of the enzyme in greater detail. wrn helicase can ca ...	1999	10373438
microsatellite alterations in superficial and locally advanced transitional cell carcinoma of the bladder.	recent studies described the existence of genetic instability associated with bladder carcinogenesis. alterations at microsatellite loci constitute a recognized tumor marker of genome instability. a series of 21 transitional cell carcinomas of the bladder (10 superficial and 11 invasive carcinomas) was analyzed for the presence of alteration in 12 microsatellite loci, in order to detect the role of microsatellite instability in genesis and progression of human bladder cancer. our preliminary res ...	1999	10373679
[assessment of sub-clinical thyroid disfunction in aged hospitalized patients in limousin].	the aims of the study were to assess the prevalence of sub-clinical thyroid dysfunction in older patients and to analyze morphological and immunological thyroid abnormalities. subjects and methods: the effect of aging on thyroid function morphology and immunology was studied in 102 french patients (70 women and 32 men) with a mean age of 81.7 +/- 7.3 years (65-101 years). all patients were hospitalized with different pathological conditions but without any thyroid disease. patients with treatmen ...	1999	10374012
[thyroid hormone determination at the public assistance hospital of paris: ordering, costs and opinions of physicians qualified in endocrinology].	to determine the distribution of orders for hormonal tests assessing thyroid function in a hospital setting. to collect the opinion of physicians specialized in endocrinology concerning free triodothyronine (ft3) assessment.	1999	10374016
vitiligo, autoimmune thyroiditis: a rare thyroid cancer arising with bone metastates on maxillofacial area.	an association between vitiligo and autoimmune thyroid disorders had previously been postulated. thyroid disorders were found in 18.5% of 15,126 patients with vitiligo, on the basis of the anamnestic data. then, we investigated 255 healthy relatives in whom we tested only t3, t4 and tsh. with the immunological investigation we detected a higher incidence of tma in vitiligo patients and in the family members. therefore, on the basis of the immunologic and thyroid pathology functional data, we obs ...	1999	10374684
unique alterations of thyroid function parameters after i.v. administration of alkylating drugs (cyclophosphamide and ifosfamide).	alkylating drugs (cyclophosphamide and ifosfamide) have been in clinical use for the treatment of malignant diseases in the past. they are most useful anticancer agents and cyclophosphamide is also widely used for its immunosuppressive properties. however the effect of alkylating drugs on thyroid hormone parameters have not been evaluated so far. three groups of patients were prospectively evaluated: group i: 15 patients with wegener's granulomatosis and 4 patients with severe scleritis received ...	1999	10376442
primary placement of a voice prosthesis on transposed colon after total pharyngolaryngoesophagectomy.	primary placement of a voice prosthesis may aid rehabilitation after total laryngectomy. methods: we present a rare clinical situation of a t4 no mo squamous cell carcinoma of the hypopharynx and esophagus in a patient who had previously undergone a transmesocolic billroth ii gastrectomy.	1999	10376757
molecular genetics of congenital hypothyroidism.	congenital thyroid gland defects - resulting in reduced production of the hormones triiodothyronine (t3) and thyroxine (t4) - can be a consequence of either reduced or absent thyroid tissue (thyroid dysgenesis) or, less frequently, of impairment in the biochemical mechanisms responsible for hormone biosynthesis (thyroid dyshormonogenesis). recent studies have revealed how mutations in the genes encoding either transcription factors or the thyroid stimulating hormone receptor cause, in humans or ...	1999	10377281
plasma lipoprotein (a) concentrations in hypothyroid, euthyroid and hyperthyroid subjects.	alterations of the lipid profile are a well known phenomenon in thyroid dysfunction. thyroid hormones regulate lipid metabolism through various mechanisms, but a key role is played by the ldl receptor pathway. thyroid hormone influence on lipoprotein (a) [lp(a)] metabolism is known.	1999	10378018
serum uric acid concentration and thyroid-stimulating-hormone (tsh): results of screening for hyperuricaemia in 2359 consecutive patients with various degrees of thyroid dysfunction.	serum uric acid concentration (sua) and hyperthyroidism have been reported to positively correlate with each other. furthermore, epidemiological data indicate that uric acid may be an independent risk factor for hypertension-associated morbidity and mortality. to evaluate whether screening for hyperuricaemia might be worthwhile in patients with hyperthyroidism we determined serum concentrations of uric acid in 2359 consecutive patients (1939 female, 420 male; age: 48 +/- 17 years, mean +/- sd) w ...	1999	10378314
effects of a low-calorie diet on resting metabolic rate and serum tri-iodothyronine levels in obese children.	the purpose of the study was to examine the effects of weight loss on resting metabolic rate (rmr) and on serum t3 levels in obese children and to investigate whether rmr changes are related to t3 changes. sixty-four healthy, overweight, children (age: 12.1+/-1.1 years, body mass index 29.3+/-4.3 kg/m2) were studied during a 6-week weight reduction programme. rmr (by indirect calorimetry) total t3, total t4, tsh and fat-free mass (ffm) (by anthropometry) were measured at baseline and after 6 wee ...	1999	10378389
differences in metallothionein expression in transplantable mouse mammary tumor lines.	in order to elucidate a possible role of metallothionein (mt) in mammary carcinogenesis, mt and sex hormone receptor (estrogen receptor, er; progesterone receptor, pr) expressions were investigated immunohistochemically in a transplantable pregnancy-dependent mouse mammary tumor (tpdmt-4) and related autonomous tumor sublines (t4-oi96, t4-oi165 and t4-oi320cy) recovered from pregnant and virgin ddd mice. tpdmt-4 showed mt expression in tumor cells, while the expression was less evident in t4-oi1 ...	1999	10378789
the heart rate turn point reliability and methodological aspects.	the aim of the study was to test protocol variations on the heart rate performance curve (hrpc) and the heart rate turn point (hrtp) according to conconi et al. (1996). respiratory gas exchange variables were used to define three phases of energy supply (i, ii, iii).	1999	10378920
serotypes and electrophoretic protein profiles of pasteurella haemolytica isolated from pneumonic ovine lungs.	serotypes and sds-page protein profiles of p. haemolytica isolated from pneumonic ovine lungs were investigated. of 268 p. haemolytica isolates, 232 (86.6%) were serotypable. a total of 12 serotypes were recognized in 20 different geographic origins of central turkey. the most common serotype was a2, followed by a7, a1 and t4. serotypes a13, a14, a16 and t15 could not be detected. in sds-page, marked differences between major bands of biotype a and t strains were found. in numerical analysis of ...	1999	10379102
mutation detection using fluorescent enzyme mismatch cleavage with t4 endonuclease vii.	mutation detection techniques are often limited by sensitivity, ease of use and short fragment lengths. enzyme mismatch cleavage (emc) is a technique capable of rapidly scanning 1 kbp fragments of dna for mutations. it relies on the ability of a bacteriophage resolvase enzyme, t4 endonuclease vii, to cleave dna at single base pair mismatches and small heteroduplex loops. originally the process was performed using radioactively labeled dna and the results analysed after denaturing polyacrylamide ...	1999	10380755
intestinal damage induced by zinc deficiency is associated with enhanced cuzn superoxide dismutase activity in rats: effect of dexamethasone or thyroxine treatment.	zinc has a wide spectrum of biological activities and its deficiency has been related to various tissue dysfunctions and alterations of normal cell metabolism. zinc also plays an important role in the antioxidant cellular defenses being a structural element of the non-mitochondrial form of the enzyme superoxide dismutase (cuznsod). we have already reported that zn deficiency induces severe alterations in the rat intestine, that are reverted by treatment with dexamethasone (dex) or thyroxine (t4) ...	1999	10381190
serum total thyroxine, total triiodothyronine, free thyroxine, and thyrotropin concentrations in epileptic dogs treated with anticonvulsants.	to determine whether administration of phenobarbital, potassium bromide, or both drugs concurrently was associated with abnormalities in baseline serum total thyroxine (t4), triiodothyronine (t3), free t4, or thyrotropin (thyroid-stimulating hormone; tsh) concentrations in epileptic dogs.	1999	10382022
solitary nodular disease and multinodular goiter: a retrospective study on suppressive versus replacement levothyroxine therapy.	the aim of the present study was to ascertain whether patients affected by solitary nodular disease of the thyroid or multinodular goiter had a different clinical outcome when treated with suppressive levo-thyroxine (l-t4) therapy rather than replacement l-t4 therapy. we evaluated, by a retrospective analysis, 36 patients who had received tsh-suppressive l-t4 therapy according to tsh value and 55 who had received replacement l-t4 therapy. fine needle aspiration cytology and thyroid scan after 13 ...	1999	10382684
thoracic pedicle screw placement guided by computed tomographic measurements.	cadaveric pedicle screw placement guided by the measurements from axial computed tomography (ct) scans in the thoracic spine was assessed in this study. axial ct scans were performed on four cadaveric thoracic spines, and the measurements included the pedicle transverse angle, inner pedicle width, and distance between the midline of the vertebra and the pedicle axis on the dorsal aspect of the lamina. with utilization of the data from ct scans, screws were directly placed into the thoracic pedic ...	1999	10382775
palatoalveolar outcome at 18 months following simultaneous primary cleft lip repair and posterior palatoplasty.	it is frequently reported that early repair of the soft palate induces narrowing of the remaining palatal cleft and thus facilitates later hard palate closure. however, to the best of our knowledge, there have been no comparative studies to test this hypothesis. the purpose of this retrospective study was to evaluate the change of palatoalveolar morphology following primary lip repair and posterior palatoplasty. dental plaster models of patients with complete unilateral cleft of lip and palate ( ...	1999	10382792
persistent efficacy of doramectin pour-on against artificially induced infections of nematodes in cattle.	two studies were conducted to determine the persistent efficacy of doramectin pour-on against an artificial, trickle challenge of mixed nematodes in calves. in each study, 42, 4-8 months old calves were randomly assigned into four groups of 10 animals each (t1-t4), plus two larval-viability monitor animals. all animals were treated with fenbendazole (10 mg kg(-1)) 14 days prior to the start of the study to clear any existing infection. doramectin pour-on at 500 microg kg(-1) was used on each ani ...	1999	10384905
molecular typing of erythromycin-resistant streptococcus pyogenes strains with the m phenotype isolated in italy.	to assess the spread of the new m phenotype, various erythromycin-resistant streptococcus pyogenes strains from three italian cities (verona, monza, florence) were characterised. each strain was analysed for the presence of genes ermam and mefa, for the ability to accumulate radioactive erythromycin in the absence of sodium arsenate, for the protein t serological type, and for the dna macrorestriction profile identified by means of pulsed-field gel electrophoresis. in a number of strains, the pr ...	1999	10385014
mimicry in primary rat hepatocyte cultures of the in vivo perivenous induction by phenobarbital of cytochrome p-450 2b1 mrna: role of epidermal growth factor and perivenous oxygen tension.	treatment of male rats with phenobarbital (pb) results in a perivenous and mid-zonal pattern of cytochrome p-450 (cyp)2b1 mrna expression within the liver acinus. the mechanism of this zonated induction is still poorly understood. in this study sinusoidal gradients of oxygen and epidermal growth factor (egf) besides those of the pituitary-dependent hormones growth hormone (gh), thyroxine (t4), and triiodothyronine (t3) were considered to be possible determinants for the zonated induction of the ...	1999	10385683
increased rate of complete eus staging of patients with esophageal cancer using the nonoptical, wire-guided echoendoscope.	incomplete endoscopic ultrasound (eus) staging procedures in patients with esophageal cancer due to obstructing malignant strictures are prone to underestimate t stage and cannot detect celiac adenopathy. eus staging in the setting of stenotic malignancies using the large caliber echoendoscope has been complicated by esophageal perforation. we report on the clinical utility of a newly developed, wire-guided echoendoscope for the complete staging of patients with esophageal cancer.	1999	10385722