Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| ixodes ricinus and its endosymbiont midichloria mitochondrii: a comparative proteomic analysis of salivary glands and ovaries. | hard ticks are hematophagous arthropods that act as vectors of numerous pathogenic microorganisms of high relevance in human and veterinary medicine. ixodes ricinus is one of the most important tick species in europe, due to its role of vector of pathogenic bacteria such as borrelia burgdorferi and anaplasma phagocytophilum, of viruses such as tick borne encephalitis virus and of protozoans as babesia spp. in addition to these pathogens, i. ricinus harbors a symbiotic bacterium, midichloria mito ... | 2015 | 26398775 |
| haemaphysalis longicornis ticks as reservoir and vector of severe fever with thrombocytopenia syndrome virus in china. | severe fever with thrombocytopenia syndrome (sfts) is an emerging hemorrhagic fever in east asia caused by sfts virus (sftsv), a newly discovered phlebovirus. the haemaphysalis longicornis tick has been suspected to be the vector of sftsv. to determine whether sftsv can be transmitted among ticks, from ticks to animals, and from animals to ticks, we conducted transmission studies between developmental stages of h. longicornis ticks and between ticks and mice. using reverse transcription pcr, we ... | 0 | 26402039 |
| first report of ivermectin resistance in field populations of rhipicephalus (boophilus) microplus (acari: ixodidae) in punjab districts of india. | the larval immersion test (lit) was used on the progenies of rhipicephalus (boophilus) microplus collected from four districts of the punjab state, india to test the resistance to ivermectin. the regression graphs of probit mortality of larval ticks plotted against log values of increasing concentrations of ivermectin were utilized for the determination of slope of mortality, lethal concentration for 50%(lc50), 95%(lc95), resistance ratios (rr50, rr95) and the resistance levels (rl). values of t ... | 2015 | 26404476 |
| development of a pan-babesia fret-qpcr and a survey of livestock from five caribbean islands. | babesia spp. are tick-borne protozoan hemoparasites and the second most common blood-borne parasites of mammals, in particular domestic animals. we used the clustal multiple alignment program and 18s rrna gene sequences of 22 babesia species from genbank to develop a pcr that could detect a wide variety of babesia spp. in a single reaction. the pan-babesia fret-qpcr we developed reliably detected b. gibsoni, b. canis, b. vogeli, b. microti, b. bovis, and b. divergens under controlled conditions ... | 2015 | 26423577 |
| integrated metabolomics, transcriptomics and proteomics identifies metabolic pathways affected by anaplasma phagocytophilum infection in tick cells. | anaplasma phagocytophilum is an emerging zoonotic pathogen that causes human granulocytic anaplasmosis. these intracellular bacteria establish infection by affecting cell function in both the vertebrate host and the tick vector, ixodes scapularis. previous studies have characterized the tick transcriptome and proteome in response to a. phagocytophilum infection. however, in the postgenomic era, the integration of omics datasets through a systems biology approach allows network-based analyses to ... | 2015 | 26424601 |
| effects of different spray formulations on the reproductive parameters of engorged rhipicephalus (boophilus) microplus females detached from experimentally infested cattle. | this present study aimed to evaluate the deleterious effects of some commercially available spray formulations (15% cypermethrin+25% chlorpyriphos+1% citronellal and 8% cypermethrin+60% ethion) on the reproductive parameters of engorged rhipicephalus (boophilus) microplus females that detached from experimentally infested cattle. the following reproductive parameters of engorged female ticks were analyzed: female weight, egg mass weight, percentage of hatchability, percentage of reduction in ovi ... | 2015 | 26427633 |
| lipopolysaccharide-induced differential expression of mirnas in male and female rhipicephalus haemaphysaloides ticks. | lipopolysaccharide (lps) stimulates the innate immune response in arthropods. in tick vectors, lps activates expression of immune genes, including those for antibacterial peptides. mirnas are 21-24 nt non-coding small rnas that regulate target mrnas at the post-transcriptional level. however, our understanding of tick innate immunity is limited to a few cellular immune reactions and some characterized immune molecules. moreover, there is little information on the regulation of the immune system ... | 2015 | 26430879 |
| development of a generic ehrlichia fret-qpcr and investigation of ehrlichioses in domestic ruminants on five caribbean islands. | the ehrlichia are obligate intracellular gram-negative tick-borne bacteria that are important human and animal pathogens. there is a need for assays to rapidly and reliably detect and differentiate the five generally recognized species into groups in a single reaction: e. canis, e. chaffeensis, e. ewingii, e. muris and e. ruminantium. | 2015 | 26438311 |
| production of serine protease inhibitors by mutagenesis and their effects on the mortality of aedes aegypti l. larvae. | dengue, transmitted primarily by the bites of infected aedes aegypti l., is transmitted to millions of individuals each year in tropical and subtropical areas. dengue control strategies are primarily based on controlling the vector, using insecticides, but the appearance of resistance poses new challenges. recently, highly selective protease inhibitors by phage display were obtained for digestive enzymes of the 4th instar larvae (l4) midgut. these mutants were not confirmed as a larvicide due to ... | 2015 | 26444542 |
| genetic characterization and molecular survey of babesia bovis, babesia bigemina and babesia ovata in cattle, dairy cattle and yaks in china. | babesiosis is an important haemoparasitic disease, caused by the infection and subsequent intra-erythrocytic multiplication of protozoa of the genus babesia that impacts the livestock industry and animal health. the distribution, epidemiology and genetic characterization of b. bigemina, b. bovis, and b. ovata in cattle in china as well as the prevalence of these protozoan agents were assessed. | 2015 | 26452623 |
| emerging tick-borne infections in mainland china: an increasing public health threat. | since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ... | 2015 | 26453241 |
| adrenergic ligands that block oviposition in the cattle tick rhipicephalus microplus affect ovary contraction. | the tyraminergic/octopaminergic system is central for the control of arthropod oviposition. previous works demonstrated that the pharmacological perturbation of this system inhibits oviposition in the cattle tick rhipicephalus microplus. in this work, we describe a physiologically active whole-mount preparation of the contractile tick ovary that allows the quantitative videometrical analysis of ovary contraction in response to different compounds. eight adrenergic ligands known to inhibit ovipos ... | 2015 | 26456007 |
| midgut proteome of an argasid tick, ornithodoros erraticus: a comparison between unfed and engorged females. | the argasid tick ornithodoros erraticus is the vector of african swine fever virus and of several borrelia species that cause human relapsing fever in the iberian peninsula. the tick midgut is part of the ectoparasite-host interface and expresses proteins that are vital for the survival of the tick. midgut proteins are therefore potential targets for drug and/or vaccine design aimed at the development of new strategies for tick control. thus, the aim of this work was the characterization of the ... | 2015 | 26459090 |
| seasonal variation and frequency distribution of ectoparasites in crossbreed cattle in southeastern brazil. | the aims of this study were to evaluate the seasonal variation and frequency distribution of rhipicephalus (boophilus) microplus, haematobia irritans, and dermatobia hominis on crossbred heifers under field conditions in the northeast of minas gerais state, southeastern brazil. from november 2007 to september 2009 (23 months), 40 heifers aged 16.6 ± 2.4 months were divided into groups a (1/4 holstein × 3/4 gir) and b (1/2 holstein × 1/2 gir) and had the monthly infestation estimated along with t ... | 2014 | 26464941 |
| assessment of the repellent effect of lippia alba essential oil and major monoterpenes on the cattle tick rhipicephalus microplus. | the control of rhipicephalus microplus (ixodida: ixodidae) is achieved using synthetic acaricides. however, resistant tick populations are widespread around the world. plant essential oils can act as repellents, keeping ticks away from hosts and decreasing the selection pressure on synthetic acaricides. the aim of this study was to evaluate the in vitro repellent effect of lippia alba essential oil on r. microplus larvae. leaves from two l. alba genotypes maintained under the same agronomic and ... | 0 | 26471008 |
| age and prior blood feeding of anopheles gambiae influences their susceptibility and gene expression patterns to ivermectin-containing blood meals. | ivermectin has been proposed as a novel malaria transmission control tool based on its insecticidal properties and unique route of acquisition through human blood. to maximize ivermectin's effect and identify potential resistance/tolerance mechanisms, it is important to understand its effect on mosquito physiology and potential to shift mosquito population age-structure. we therefore investigated ivermectin susceptibility and gene expression changes in several age groups of female anopheles gamb ... | 2015 | 26471037 |
| lysosome-associated membrane glycoprotein (lamp)--preliminary study on a hidden antigen target for vaccination against schistosomiasis. | our previously reported gene atlasing of schistosome tissues revealed transcripts that were highly enriched in the digestive tract of schistosoma mansoni. from these, we selected two candidates, sm-lamp and sm-npc2 for testing as vaccine targets. the two molecules were selected on the basis of relatively high expression in the gastrodermis, their potentially important biological function, divergence from homologous molecules of the host and possible apical membrane expression in the gastrodermis ... | 2015 | 26472258 |
| divergent viruses discovered in arthropods and vertebrates revise the evolutionary history of the flaviviridae and related viruses. | viruses of the family flaviviridae are important pathogens of humans and other animals and are currently classified into four genera. to better understand their diversity, evolutionary history, and genomic flexibility, we used transcriptome sequencing (rna-seq) to search for the viruses related to the flaviviridae in a range of potential invertebrate and vertebrate hosts. accordingly, we recovered the full genomes of five segmented jingmenviruses and 12 distant relatives of the known flavivirida ... | 2015 | 26491167 |
| integration of ixodes ricinus genome sequencing with transcriptome and proteome annotation of the naïve midgut. | in europe, ixodes ricinus ticks are the most important vectors of diseases threatening humans, livestock, wildlife and companion animals. nevertheless, genomic sequence information is missing and functional annotation of transcripts and proteins is limited. this lack of information is restricting studies of the vector and its interactions with pathogens and hosts. here we present and integrate the first analysis of the i. ricinus genome with the transcriptome and proteome of the unfed i. ricinus ... | 2015 | 26510422 |
| effective inhibition of thrombin by rhipicephalus microplus serpin-15 (rms-15) obtained in the yeast pichia pastoris. | the cattle tick (rhipicephalus microplus) affects cattle industries in tropical and subtropical countries because it is the vector of babesiosis and anaplasmosis which constitutes a threat to the health of cattle. during blooding feeding, ticks secrete saliva containing a complex of bioactive molecules into the injured site to evade host's defensive responses. serine protease inhibitors (serpins) are important anti-haemostatic molecules present in tick saliva that are necessary for a successful ... | 2016 | 26530984 |
| effect of malathion on reproductive parameters of engorged female rhipicephalus (boophilus) microplus ticks of punjab districts, india. | the present study was aimed at evaluating effects of malathion on the various reproductive parameters, namely, egg mass weight (emw), reproductive index (ri), percentage inhibition of oviposition (%io), and hatchability percentage of eggs of rhipicephalus (boophilus) microplus (canestrini 1887) females from 19 districts of punjab, india. the effect on various parameters was found to be dose dependent and more discernible upon exposure to higher concentrations. complete cessation of egg laying wa ... | 2015 | 26550485 |
| global transcriptome analysis of the tentacle of the jellyfish cyanea capillata using deep sequencing and expressed sequence tags: insight into the toxin- and degenerative disease-related transcripts. | jellyfish contain diverse toxins and other bioactive components. however, large-scale identification of novel toxins and bioactive components from jellyfish has been hampered by the low efficiency of traditional isolation and purification methods. | 2015 | 26551022 |
| ten years later: evaluation of the effectiveness of 12.5% amitraz against a field population of rhipicephalus (boophilus) microplus using field studies, artificial infestation (stall tests) and adult immersion tests. | using field trials, artificial infestations (stall tests) and in vitro adult immersion tests, the present study evaluated the acaricidal efficacy of 12.5% amitraz administered via whole body spraying against a rhipicephalus (boophilus) microplus population that did not have any contact with chemical products belonging to this acaricide family for 10 years (approximately 40 generations). two natural infestation trials, two artificial infestation trials (stall tests) and two adult immersion tests ... | 2015 | 26559460 |
| molecular evidence of spotted fever group rickettsiae and anaplasmataceae from ticks and stray dogs in bangladesh. | emerging tick-borne diseases (tbds) are important foci for human and animal health worldwide. however, these diseases are sometimes over looked, especially in countries with limited resources to perform molecular-based surveys. the aim of this study was to detect and characterize spotted fever group (sfg) rickettsiae and anaplasmataceae in bangladesh, which are important tick-borne pathogens for humans and animals worldwide. a total of 50 canine blood samples, 15 ticks collected from dogs, and 1 ... | 2016 | 26573516 |
| length and density of filiform tongue papillae: differences between tick-susceptible and resistant cattle may affect tick loads. | indicine breeds of bovines are highly resistant and taurine breeds are susceptible to the cattle tick, rhipicephalus microplus, a species which causes great damage to livestock. animals use their tongues for self-grooming, an important behavior for ridding themselves of ectoparasites. however, the role of tongue morphology, notably the filiform papillae, in this process is not known. | 2015 | 26573922 |
| insights into the development of ixodes scapularis: a resource for research on a medically important tick species. | ticks (acari: ixodida) are arthropod ectoparasites dependent on a bloodmeal from a vertebrate host at each developmental stage for completion of their life cycle. this tick feeding cycle impacts animal health by causing damage to hides, secondary infections, immune reactions and diseases caused by transmission of pathogens. the genus ixodes includes several medically important species that vector diseases, including granulocytic anaplasmosis and lyme disease. i. scapularis, commonly called the b ... | 2015 | 26576940 |
| wolbachia and the insect immune system: what reactive oxygen species can tell us about the mechanisms of wolbachia-host interactions. | wolbachia are intracellular bacteria that infect a vast range of arthropod species, making them one of the most prevalent endosymbionts in the world. wolbachia's stunning evolutionary success is mostly due to their reproductive parasitism but also to mutualistic effects such as increased host fecundity or protection against pathogens. however, the mechanisms underlying wolbachia phenotypes, both parasitic and mutualistic, are only poorly understood. moreover, it is unclear how the insect immune ... | 2015 | 26579107 |
| ixodes scapularis and ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis. | ixodid ticks are important vectors of a wide variety of viral, bacterial and protozoan pathogens of medical and veterinary importance. although several studies have elucidated tick responses to bacteria, little is known about the tick response to viruses. to gain insight into the response of tick cells to flavivirus infection, the transcriptomes and proteomes of two ixodes spp cell lines infected with the flavivirus tick-borne encephalitis virus (tbev) were analysed. | 2015 | 26582129 |
| borrelia miyamotoi disease: neither lyme disease nor relapsing fever. | borrelia miyamotoi disease (bmd) is a newly recognized borreliosis globally transmitted by ticks of the ixodes persulcatus species complex. once considered to be a tick symbiont with no public health implications, b miyamotoi is increasingly recognized as the agent of a nonspecific febrile illness often misdiagnosed as acute lyme disease without rash, or as ehrlichiosis. the frequency of its diagnosis in the northeastern united states is similar to that of human granulocytic ehrlichiosis. a diag ... | 2015 | 26593262 |
| identification and characterization of a cathepsin l-like cysteine protease from rhipicephalus (boophilus) annulatus. | the tick rhipicephalus (boophilus) annulatus is one of the most important ectoparasites of bovines and is responsible for the transmission of different pathogens such as babesia and anaplasma. cysteine proteases are involved in several host-tick interactions including invasion of host tissues, immune evasion, pathogen transmission, embryogenesis and blood digestion. in this study, the gene encoding r. annulatus cathepsin l-like enzyme (racl1) was cloned into ptz57r/t vector, sequenced and analyz ... | 2016 | 26597589 |
| antileishmanial activity of the essential oil from tetradenia riparia obtained in different seasons. | the herbaceous shrub tetradenia riparia has been traditionally used to treat inflammatory and infectious diseases. recently, a study showed that t. riparia essential oil (treo) obtained in summer has antileishmanial effects, although these results could be influenced by seasonal variation. this study evaluated the activity of the treo obtained in different seasons against leishmania (leishmania) amazonensis, in vitro and in vivo. the compounds in the treo were analysed by gas chromatography-mass ... | 2015 | 26602873 |
| emerging horizons for tick-borne pathogens: from the 'one pathogen-one disease' vision to the pathobiome paradigm. | ticks, as vectors of several notorious zoonotic pathogens, represent an important and increasing threat for human and animal health in europe. recent applications of new technology revealed the complexity of the tick microbiome, which may affect its vectorial capacity. appreciation of these complex systems is expanding our understanding of tick-borne pathogens, leading us to evolve a more integrated view that embraces the 'pathobiome'; the pathogenic agent integrated within its abiotic and bioti ... | 2015 | 26610021 |
| nellore cattle (bos indicus) and ticks within the brazilian pantanal: ecological relationships. | pantanal is a huge floodplain mostly in brazil, and its main economic activity is extensive cattle raising, in farms characterized by an extremely wildlife-rich environment. we herein describe tick infestations of cattle and of the natural environment in pantanal of nhecolândia in brazil, at areas with and without cattle during both dry and wet seasons. environmental sampling resulted in three tick species: amblyomma sculptum (423 nymphs and 518 adults), amblyomma parvum (7 nymphs and 129 adults ... | 2016 | 26613758 |
| targeted silencing of the aquaporin 2 gene of rhipicephalus (boophilus) microplus reduces tick fitness. | ticks are blood-feeding arthropods that can affect human and animal health both directly by blood-feeding and indirectly by transmitting pathogens. the cattle tick rhipicephalus (boophilus) microplus is one of the most economically important ectoparasites of bovines worldwide and it is responsible for the transmission of the protozoan babesia bovis, the etiological agent of bovine babesiosis. aquaporins (aqps) are water channel proteins implicated in physiological mechanisms of osmoregulation. m ... | 2015 | 26626727 |
| the wild side of disease control at the wildlife-livestock-human interface: a review. | the control of diseases shared with wildlife requires the development of strategies that will reduce pathogen transmission between wildlife and both domestic animals and human beings. this review describes and criticizes the options currently applied and attempts to forecast wildlife disease control in the coming decades. establishing a proper surveillance and monitoring scheme (disease and population wise) is the absolute priority before even making the decision as to whether or not to interven ... | 2014 | 26664926 |
| mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, streptomyces sp. m25 against anopheles subpictus, culex quinquefasciatus and aedes aegypti. | the present work reports the larvicidal potential of microbially synthesised silver nanoparticles (agnps) by using an actinobacterium, streptomyces sp. m25 isolated from western ghats, tamil nadu, india. the biomass of streptomyces sp. was exposed to 1 mm silver nitrate (agno3) solution. the synthesis of agnps was confirmed by visual inspection followed by instrumental analysis such as uv-vis spectroscopy, fourier transform infrared spectroscopy, x-ray diffraction (xrd), energy dispersive x-ray ... | 2013 | 26688633 |
| a review of reverse vaccinology approaches for the development of vaccines against ticks and tick borne diseases. | the field of reverse vaccinology developed as an outcome of the genome sequence revolution. following the introduction of live vaccinations in the western world by edward jenner in 1798 and the coining of the phrase 'vaccine', in 1881 pasteur developed a rational design for vaccines. pasteur proposed that in order to make a vaccine that one should 'isolate, inactivate and inject the microorganism' and these basic rules of vaccinology were largely followed for the next 100 years leading to the el ... | 2016 | 26723274 |
| chemo-profiling and bioassay of phytoextracts from ageratum conyzoides for acaricidal properties against rhipicephalus (boophilus) microplus (acari: ixodidae) infesting cattle and buffaloes in india. | in india, rhipicephalus (boophilus) microplus populations have developed a certain level of resistance to most of the acaricides marketed against tick species. to manage the problem, the present study was undertaken to evaluate the acaricidal potential of ageratum conyzoides plants against acaricides-resistant ticks infesting cattle and buffaloes. the regression analysis of dose-response data of ethanolic extract of a. conyzoides revealed lc90 value of 8.91% against reference susceptible ivri-1 ... | 2016 | 26723275 |
| exploring the immune signalling pathway-related genes of the cattle tick rhipicephalus microplus: from molecular characterization to transcriptional profile upon microbial challenge. | in dipteran insects, invading pathogens are selectively recognized by four major pathways, namely toll, imd, jnk, and jak/stat, and trigger the activation of several immune effectors. although substantial advances have been made in understanding the immunity of model insects such as drosophila melanogaster, knowledge on the activation of immune responses in other arthropods such as ticks remains limited. herein, we have deepened our understanding of the intracellular signalling pathways likely t ... | 2016 | 26724380 |
| emergence of multi-acaricide resistant rhipicephalus ticks and its implication on chemical tick control in uganda. | acaricide failure has been on the rise in the western and central cattle corridor of uganda. in this study, we identified the tick species associated with acaricide failure and determined their susceptibility to various acaricide molecules used for tick control in uganda. | 2016 | 26727991 |
| mitochondrial genome evolution and trna truncation in acariformes mites: new evidence from eriophyoid mites. | the subclass acari (mites and ticks) comprises two super-orders: acariformes and parasitiformes. most species of the parasitiformes known retained the ancestral pattern of mitochondrial (mt) gene arrangement of arthropods, and their mt trnas have the typical cloverleaf structure. all of the species of the acariformes known, however, have rearranged mt genomes and truncated mt trnas. we sequenced the mt genomes of two species of eriophyoidea: phyllocoptes taishanensis and epitrimerus sabinae. the ... | 2016 | 26732998 |
| in vitro mode of action and anti-thrombotic activity of boophilin, a multifunctional kunitz protease inhibitor from the midgut of a tick vector of babesiosis, rhipicephalus microplus. | hematophagous mosquitos and ticks avoid host hemostatic system through expression of enzyme inhibitors targeting proteolytic reactions of the coagulation and complement cascades. while most inhibitors characterized to date were found in the salivary glands, relatively few others have been identified in the midgut. among those, boophilin is a 2-kunitz multifunctional inhibitor targeting thrombin, elastase, and kallikrein. however, the kinetics of boophilin interaction with these enzymes, how it m ... | 2016 | 26745503 |
| first isolation of severe fever with thrombocytopenia syndrome virus from haemaphysalis longicornis ticks collected in severe fever with thrombocytopenia syndrome outbreak areas in the republic of korea. | severe fever with thrombocytopenia syndrome (sfts) is an emerging tick-borne infectious disease that is endemic to china, japan, and the republic of korea (rok). in this study, 8313 ticks collected from sfts outbreak areas in the rok in 2013 were used to detect the sfts virus (sftsv). a single sftsv was isolated in cell culture from one pool of haemaphysalis longicornis ticks collected from samcheok-si, gangwon province, in the rok. phylogenetic analysis showed that the sftsv isolate was cluster ... | 2016 | 26745758 |
| ixodes scapularis tick saliva proteins sequentially secreted every 24 h during blood feeding. | ixodes scapularis is the most medically important tick species and transmits five of the 14 reportable human tick borne disease (tbd) agents in the usa. this study describes lc-ms/ms identification of 582 tick- and 83 rabbit proteins in saliva of i. scapularis ticks that fed for 24, 48, 72, 96, and 120 h, as well as engorged but not detached (bd), and spontaneously detached (sd). the 582 tick proteins include proteases (5.7%), protease inhibitors (7.4%), unknown function proteins (22%), immunity ... | 2016 | 26751078 |
| insecticide resistance and management strategies in urban ecosystems. | the increased urbanization of a growing global population makes imperative the development of sustainable integrated pest management (ipm) strategies for urban pest control. this emphasizes pests that are closely associated with the health and wellbeing of humans and domesticated animals. concurrently there are regulatory requirements enforced to minimize inadvertent exposures to insecticides in the urban environment. development of insecticide resistance management (irm) strategies in urban eco ... | 2016 | 26751480 |
| the microbiome of ehrlichia-infected and uninfected lone star ticks (amblyomma americanum). | the lone star tick, amblyomma americanum, transmits several bacterial pathogens including species of anaplasma and ehrlichia. amblyomma americanum also hosts a number of non-pathogenic bacterial endosymbionts. recent studies of other arthropod and insect vectors have documented that commensal microflora can influence transmission of vector-borne pathogens; however, little is known about tick microbiomes and their possible influence on tick-borne diseases. our objective was to compare bacterial c ... | 2016 | 26751816 |
| ehrlichia minasensis sp. nov., a new species within the genus ehrlichia isolated from the tick rhipicephalus microplus. | recently, we obtained a rickettsial isolate (ehrlichia sp. ufmg-ev) from the hemolymph of rhipicephalus microplus engorged tick females. on the basis of maximum likelihood phylogenetic analysis using 16s rrna, groel, dsb, glta and trp36 nucleotide sequences we showed that ehrlichia sp. ufmg-ev belongs to the α-proteobacteria, family anaplasmataceae, genus ehrlichia. ehrlichia sp. ufmg-ev is a sister taxa of e. canis with 16s rrna, groel, dsb, glta and trp36 nucleotide sequence similarity of 98.3 ... | 2016 | 26763978 |
| genotype by environment interaction for tick resistance of hereford and braford beef cattle using reaction norm models. | the cattle tick is a parasite that adversely affects livestock performance in tropical areas. although countries such as australia and brazil have developed genetic evaluations for tick resistance, these evaluations have not considered genotype by environment (g*e) interactions. genetic gains could be adversely affected, since breedstock comparisons are environmentally dependent on the presence of g*e interactions, particularly if residual variability is also heterogeneous across environments. t ... | 2016 | 26767704 |
| therapeutic potential of tea tree oil for scabies. | globally, scabies affects more than 130 million people at any time. in the developed world, outbreaks in health institutions and vulnerable communities result in a significant economic burden. a review of the literature demonstrates the emergence of resistance toward classical scabicidal treatments and the lack of effectiveness of currently available scabicides in reducing the inflammatory skin reactions and pyodermal progression that occurs in predisposed patient cohorts. tea tree oil (tto) has ... | 2016 | 26787146 |
| in vitro detection of acaricidal resistance status of rhipicephalus (boophilus) microplus against commercial preparation of deltamethrin, flumethrin, and fipronil from north gujarat, india. | rhipicephalus (boophilus) microplus is the most common tick species in india infesting cattle and buffaloes and causing significant economic losses to dairy and leather industries by adversely affecting the milk production and quality of hides. a study to evaluate the acaricide resistance status of rhipicephalus (boophilus) microplus to deltamethrin, flumethrin, and fipronil was conducted on the samples collected from organized and unorganized farms of north gujarat state, where treatment failur ... | 2015 | 26788362 |
| eprinomectin accumulation in rhipicephalus (boophilus) microplus: pharmacokinetic and efficacy assessment. | eprinomectin (epm) is a macrocyclic lactone used against endo-ectoparasites without withdrawal time in milk and meat after its pour-on administration at 0.5mg/kg. previous experiments evaluated the efficacy of epm against rhipicephalus (boophilus) microplus in cattle. this study assessed epm efficacy against r. (b.) microplus after topical administration at two dose rates and investigated the relationship between epm systemic exposure in the host and drug concentrations accumulated in ticks reco ... | 2016 | 26790731 |
| tick salivary cholinesterase: a probable immunomodulator of host-parasite interactions. | the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini), is the most economically important cattle ectoparasite in the world. rhipicephalus microplus and rhipicephalus annulatus (say) continue to threaten u.s. cattle producers despite eradication and an importation barrier based on inspection, dipping of imported cattle in organophosphate (op) acaricide, and quarantine of infested premises. op acaricides inhibit acetylcholinesterase (ache), essential to tick central nervous sy ... | 2016 | 26794231 |
| invitro acaricidal activity of ethnoveterinary plants and green synthesis of zinc oxide nanoparticles against rhipicephalus (boophilus) microplus. | the present study was designed to investigate the invitro acaricidal effects of seven ethnoveterinary plants, zinc acetate and green synthesized zinc oxide nanoparticles against the southern cattle tick, rhipicephalus (boophilus) microplus. the selected ethnoveterinary plants were extracted using ethanol and aqueous (water) solvents at 0.02mg/ml and 0.04mg/ml concentrations. of these seven plants, lobelia leschenaultiana showed the highest percentage of tick mortality. the ethanol extracts of l. ... | 2016 | 26801601 |
| multiple mutations in the acetylcholinesterase 3 gene associated with organophosphate resistance in rhipicephalus (boophilus) microplus ticks from punjab, india. | the organophosphate (op) resistance status in rhipicephalus (boophilus) microplus ticks collected from seventeen districts located in the northwestern indian state, punjab were characterized using three data sets (bioassay, biochemical and molecular assays). adult immersion test (ait) was adopted and the resistance factors (rf) for the field isolates were determined. resistance to malathion was detected in 12 isolates among which 11 showed level i resistance status while level ii status was reco ... | 2016 | 26801603 |
| community structure, seasonal variations and interactions between native and invasive cattle tick species in benin and burkina faso. | the variation of tick abundance on ruminants had received little attention in west africa before rhipicephalus (boophilus) microplus started to invade this region in the early 2000s. ten years later, r. microplus was suspected to have replaced the native ticks. in addition to testing this hypothesis, this study investigated the interactions between native and invasive ticks and the relative role of climatic and geographical variables in the variations of tick community composition (beta diversit ... | 2016 | 26819238 |
| survey of cattle tick, riphicephalus (boophilus) microplus, resistance to amitraz and deltamethrin in new caledonia. | the evolution of tick resistance to acaricides in new caledonia was followed in two ways: through two large scale surveys in 1998 and 2014 and through the routine analysis of samples of ticks with suspected resistance. the results of the two approaches were qualitatively similar though analysis of ticks with suspected resistance gave higher frequencies of resistance, as expected of a biased sample. resistance tests using a larval packet test have been conducted since 1993 for deltamethrin and 20 ... | 2016 | 26827863 |
| linkage disequilibrium, persistence of phase and effective population size estimates in hereford and braford cattle. | the existence of moderate to high levels of linkage disequilibrium (ld) between genetic markers and quantitative trait loci (qtl) affecting traits of interest is fundamental for the success of genome-wide association (gwas) and genomic selection (gs) studies. knowledge about the extent and the pattern of ld in livestock populations is essential to determine the density of single nucleotide polymorphisms (snp) required for accurate gwas and gs. moreover, observed ld is related to historical effec ... | 2016 | 26832943 |
| in vitro activity of 3β-o-tigloylmelianol from guarea kunthiana a. juss (meliaceae) on oogenesis and ecdysis of the cattle tick rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae). | we evaluated the effects of 3β-o-tigloylmelianol from guarea kunthiana a. juss (meliaceae) on oogenesis, as a larvicide and on ecdysis of the larvae and the nymphs of the cattle tick rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae). on the oogenesis' test, 48 engorged females were divided into three groups, evaluated at 24, 48 and 72 h post-treatment. half of the females were treated with 0.01% 3β-o-tigloylmelianol diluted in distilled water and 5% dimethyl sulfoxide (dmso), wh ... | 2016 | 26844755 |
| the putative role of rhipicephalus microplus salivary serpins in the tick-host relationship. | inflammation and hemostasis are part of the host's first line of defense to tick feeding. these systems are in part serine protease mediated and are tightly controlled by their endogenous inhibitors, in the serpin superfamily (serine protease inhibitors). from this perspective ticks are thought to use serpins to evade host defenses during feeding. the cattle tick rhipicephalus microplus encodes at least 24 serpins, of which rms-3, rms-6, and rms-17 were previously identified in saliva of this ti ... | 2016 | 26844868 |
| isolation of the monooxygenase complex from rhipicephalus (boophilus) microplus - clues to understanding acaricide resistance. | the monooxygenase complex is composed of three key proteins, a cytochrome p450 (cyp), the cytochrome p450 oxidoreductase (cpr) and cytochrome b5 and plays a key role in the metabolism and detoxification of xenobiotic substances, including pesticides. in addition, overexpression of these components has been linked to pesticide resistance in several important vectors of disease. despite this, the monooxygenase complex has not been isolated from the southern cattle tick rhipicephalus (boophilus) mi ... | 2016 | 26850353 |
| an integrative approach to understanding pyrethroid resistance in rhipicephalus microplus and r. decoloratus ticks. | rhipicephalus microplus and rhipicephalus decoloratus species occur in regions with savannah and temperate climates, typically in grassland and wooded areas used as cattle pasture. both species are associated with the transmission of anaplasma and babesia spp., impacting livestock health and quality of livestock-associated products. in africa, tick control is predominantly mediated with the use of acaricides, such as synthetic pyrethroids. after several years on the market, reports of resistance ... | 2016 | 26851840 |
| efficacy of 11 brazilian essential oils on lethality of the cattle tick rhipicephalus (boophilus) microplus. | herbal extracts have been investigated as an alternative for parasite control, aiming to slow the development of resistance and to obtain low-cost biodegradable parasiticides. the goal of this study was to evaluate the efficacy, in vitro, of 11 essential oils from brazil on reproductive efficiency and lethality of the cattle tick rhipicephalus (boophilus) microplus. the effects of oils extracted from curcuma longa, zingiber officinale, lippia alba, lippia gracilis, lippia origanoides, lippia sid ... | 2016 | 26867819 |
| temporal gene expression analysis and rna silencing of single and multiple members of gene family in the lone star tick amblyomma americanum. | saliva is an integral factor in the feeding success of veterinary and medically important ticks. therefore, the characterization of the proteins present in tick saliva is an important area of tick research. here, we confirmed previously generated sialotranscriptome data using quantitative real-time pcr. the information obtained in this in-depth study of gene expression was used to measure the effects of metalloprotease gene silencing on tick feeding. we analyzed the temporal expression of seven ... | 2016 | 26872360 |
| genome-wide association study of tick resistance in south african nguni cattle. | ticks and tick-borne diseases are among the main causes of economic loss in the south african cattle industry through high morbidity and mortality rates. concerns of the general public regarding chemical residues may tarnish their perceptions of food safety and environmental health when the husbandry of cattle includes frequent use of acaricides to manage ticks. the primary objective of this study was to identify single nucleotide polymorphism (snp) markers associated with host resistance to tic ... | 2016 | 26897394 |
| missed retained tick: "look for the legs". | 2015 | 26900234 | |
| babesia bovis and babesia bigemina infection levels estimated by qpcr in angus cattle from an endemic area of são paulo state, brazil. | the levels of infection by babesia bovis and babesia bigemina were estimated by absolute quantification through the quantitative pcr technique (qpcr). fifty-one contemporaneous angus cattle were evaluated on two occasions. the number of standard female rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant edta. the blood samples were submitted to dna extraction and used to quantify th ... | 2016 | 26907097 |
| pfirr interacts with hrigf-i and activates the map-kinase and pi3-kinase signaling pathways to regulate glycogen metabolism in pinctada fucata. | the insulin-induced mitogen-activated protein kinase (mapk) and phosphatidylinositol 3-kinase (pi3k) pathways are major intracellular signaling modules and conserved among eukaryotes that are known to regulate diverse cellular processes. however, they have not been investigated in the mollusk species pinctada fucata. here, we demonstrate that insulin-related peptide receptor of p. fucata (pfirr) interacts with human recombinant insulin-like growth factor i (hrigf-i), and stimulates the mapk and ... | 2016 | 26911653 |
| cattle tick vaccine researchers join forces in catvac. | a meeting sponsored by the bill & melinda gates foundation was held at the avanti hotel, mohammedia, morocco, july 14-15, 2015. the meeting resulted in the formation of the cattle tick vaccine consortium (catvac). | 2016 | 26911668 |
| bmti-a, a kunitz type inhibitor from rhipicephalus microplus able to interfere in vessel formation. | rhipicephalus microplus is an ectoparasite responsible for transmissions of babesiosis and anaplasmosis causing large losses to livestock production. to survive r. microplus tick produces several active molecules, such as protease inhibitors. this ectoparasite has been described as a rich source of serine protease inhibitors most of them are kunitz-bpti members named bmtis which have no clear function yet. in the present work, we described the expression and functional characterization of rbmti- ... | 2016 | 26921038 |
| prediction of g protein-coupled receptor encoding sequences from the synganglion transcriptome of the cattle tick, rhipicephalus microplus. | the cattle tick, rhipicephalus (boophilus) microplus, is a pest which causes multiple health complications in cattle. the g protein-coupled receptor (gpcr) super-family presents a candidate target for developing novel tick control methods. however, gpcrs share limited sequence similarity among orthologous family members, and there is no reference genome available for r. microplus. this limits the effectiveness of alignment-dependent methods such as blast and pfam for identifying gpcrs from r. mi ... | 2016 | 26922323 |
| molecular detection of rickettsia, anaplasma, coxiella and francisella bacteria in ticks collected from artiodactyla in thailand. | a total of 79 ticks collected from sambar deer (cervus unicolor), barking deer (muntiacus muntjak) and wild boar (sus scrofa) were examined by pcr for the presence of rickettsia, anaplasma, coxiella, and francisella bacteria. of the 79 ticks, 13% tested positive for rickettsia, 15% tested positive for anaplasma, 4% tested positive for coxiella, and 3% tested positive for francisella. interestingly, triple infection with anaplasma, rickettsia and francisella was determined in a dermacentor auratu ... | 2016 | 26934997 |
| molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs. | an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ... | 2016 | 26943995 |
| acquisition of exogenous haem is essential for tick reproduction. | haem and iron homeostasis in most eukaryotic cells is based on a balanced flux between haem biosynthesis and haem oxygenase-mediated degradation. unlike most eukaryotes, ticks possess an incomplete haem biosynthetic pathway and, together with other (non-haematophagous) mites, lack a gene encoding haem oxygenase. we demonstrated, by membrane feeding, that ticks do not acquire bioavailable iron from haemoglobin-derived haem. however, ticks require dietary haemoglobin as an exogenous source of haem ... | 2016 | 26949258 |
| genetic diversity, acaricide resistance status and evolutionary potential of a rhipicephalus microplus population from a disease-controlled cattle farming area in south africa. | the southern cattle tick, rhipicephalus microplus is a hematophagous ectoparasite of great veterinary and economic importance. along with its adaptability, reproductive success and vectoring capacity, r. microplus has been reported to develop resistance to the major chemical classes of acaricides currently in use. in south africa, the mnisi community in the mpumalanga region offers a unique opportunity to study the adaptive potential of r. microplus. the aims of this study therefore included cha ... | 2016 | 26965787 |
| genetic diversity and molecular characterization of babesia motasi-like in small ruminants and ixodid ticks from china. | ovine babesioses, an important tick-borne disease of sheep and goats in china, is caused by the reproduction of intraerythrocytic protozoa of the babesia genus. babesia motasi-like is a babesia parasite that infects small ruminant in china, and two sub-groups of b. motasi-like can be subdivided based on differences in the rhoptry-associated-protein-1 gene. this study aimed to characterize the distribution, epidemiology and genetics of b. motasi-like in animals and ticks. a molecular investigatio ... | 2016 | 26976477 |
| esterase mediated resistance in deltamethrin resistant reference tick colony of rhipicephalus (boophilus) microplus. | monitoring of acaricide resistance is considered as one of the important facets of integrated tick management. in an attempt of development of resistance monitoring indicators, in the present study two reference tick lines of rhipicephalus (boophilus) microplus maintained in the entomology laboratory, indian veterinary research institute (ivri), izatnagar, india, were studied to determine the possible contributing factors involved in development of resistance to deltamethrin. electrophoretic pro ... | 2016 | 26979585 |
| matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of east african ixodid tick species. | the tick population of africa includes several important genera belonging to the family ixodidae. many of these ticks are vectors of protozoan and rickettsial pathogens including theileria parva that causes east coast fever, a debilitating cattle disease endemic to eastern, central and southern africa. effective surveillance of tick-borne pathogens depends on accurate identification and mapping of their tick vectors. a simple and reproducible technique for rapid and reliable differentiation of l ... | 2016 | 26979606 |
| alterations in the oxidative metabolism of rhipicephalus (boophilus) microplus ticks in response to exposure to the insect growth regulator fluazuron. | aiming to characterize the potential off-target effects of fluazuron on ticks, biochemical analyses were conducted to evaluate changes in the carbohydrate metabolism of rhipicephalus (boophilus) microplus ticks after exposure to fluazuron. hemolymph and fat body were collected from female ticks before and after (4, 8 and 15 days) exposure to fluazuron. spectrophotometric analyses were done to quantify glucose concentration and lactate dehydrogenase (ldh) activity in the hemolymph and the concent ... | 2016 | 26982563 |
| effects of passages through a suitable host of the fungus, metarhizium anisopliae, on the virulence of acaricide-susceptible and resistant strains of the tick, rhipicephalus microplus. | the aim of this work was to assess the virulence of strain m379 of the fungus, metarhizium anisopliae (metchnikoff) sorokin (hypocreales: clavicipitaceae) after different passages through a suitable host and at different concentrations for the control of both acaricide-susceptible and resistant strains of the tick, rhipicephalus (formerly boophilus) microplus canestrini (ixodida:ixodidae) in vitro. the highest value of lc50 for the susceptible strain corresponded to zero passage with 7.68 × 107 ... | 2011 | 26983168 |
| paralog analyses reveal gene duplication events and genes under positive selection in ixodes scapularis and other ixodid ticks. | hard ticks (family ixodidae) are obligatory hematophagous ectoparasites of worldwide medical and veterinary importance. the haploid genomes of multiple species of ixodid ticks exceed 1 gbp, prompting questions regarding gene, segmental and whole genome duplication in this phyletic group. the availability of the genome assembly for the black legged tick, ixodes scapularis, and transcriptome datasets for multiple species of ticks offers an opportunity to assess the contribution of gene duplication ... | 2016 | 26984180 |
| molecular detection and identification of piroplasms in sika deer (cervus nippon) from jilin province, china. | piroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera theileria and babesia. wildlife such as sika deer play an important role as reservoir hosts for several species of theileria and babesia. using blood samples collected from sika deer, we investigated the epidemiology of theileria spp. and babesia spp. in sika deer from jilin province in china and identified those species that cause pathogenic infections in sika deer. | 2016 | 26984286 |
| injectable fipronil for cattle: plasma disposition and efficacy against rhipicephalus microplus. | fipronil is a phenylpyrazole class insecticide. it is widely used as an insecticide in agriculture and in the control of ectoparasites in veterinary medicine. the application of fipronil in an injectable form (subcutaneously) becomes an innovation, since there is no commercially available preparation containing fipronil herein. the present study aimed at fipronil usage, applied subcutaneously in cattle, to control rhipicephalus microplus. the assessing criteria used in the research have been the ... | 2016 | 26995714 |
| population dynamics and evaluation of the partial selective treatment of crossbreed steers naturally infested with rhipicephalus (boophilus) microplus in a herd from the state of minas gerais in brazil. | the present study aimed to evaluate the population dynamics of rhipicephalus (boophilus) microplus over a period of 13 months on a rural property located in the state of minas gerais in southeastern brazil. animals were treated for ticks indoors by whole body spraying when r. (b.) microplus had an average count equal or more than 30 ticks. the study also evaluated the possibility of a partial selective treatment for bovines to control r. (b.) microplus in which only a percentage of the populatio ... | 2016 | 26995724 |
| marine natural products from new caledonia--a review. | marine micro- and macroorganisms are well known to produce metabolites with high biotechnological potential. nearly 40 years of systematic prospecting all around the new caledonia archipelago and several successive research programs have uncovered new chemical leads from benthic and planktonic organisms. after species identification, biological and/or pharmaceutical analyses are performed on marine organisms to assess their bioactivities. a total of 3582 genera, 1107 families and 9372 species ha ... | 2016 | 26999165 |
| insects for breakfast and whales for dinner: the diet and body condition of dingoes on fraser island (k'gari). | top-predators play stabilising roles in island food webs, including fraser island, australia. subsidising generalist predators with human-sourced food could disrupt this balance, but has been proposed to improve the overall health of the island's dingo (canis lupus dingo) population, which is allegedly 'starving' or in 'poor condition'. we assess this hypothesis by describing the diet and health of dingoes on fraser island from datasets collected between 2001 and 2015. medium-sized mammals (such ... | 2016 | 27009879 |
| survival and immune response of the chagas vector meccus pallidipennis (hemiptera: reduviidae) against two entomopathogenic fungi, metarhizium anisopliae and isaria fumosorosea. | chagas disease is a key health problem in latin america and is caused and transmitted by trypanosoma cruzi and triatomine bugs, respectively. control of triatomines has largely relied on the use pyrethroids, which has proved to be ineffective in the long term. alternatively, the use of entomopathogenic fungi has been implemented to control triatomine bugs. these fungi are highly efficient as they induce a reduction in immune response on insects. meccus pallidipennis is the main triatomine vector ... | 2016 | 27012246 |
| going beyond the control of quorum-sensing to combat biofilm infections. | most bacteria attach to surfaces where they form a biofilm, cells embedded in a complex matrix of polymers. cells in biofilms are much better protected against noxious agents than free-living cells. as a consequence it is very difficult to control pathogens with antibiotics in biofilm infections and novel targets are urgently needed. one approach aims at the communication between cells to form and to maintain a biofilm, a process called quorum-sensing. water soluble small-sized molecules mediate ... | 2016 | 27025518 |
| severe fever with thrombocytopenia syndrome virus antigen detection using monoclonal antibodies to the nucleocapsid protein. | severe fever with thrombocytopenia syndrome (sfts) is a tick-borne infectious disease with a high case fatality rate, and is caused by the sfts virus (sftsv). sfts is endemic to china, south korea, and japan. the viral rna level in sera of patients with sfts is known to be strongly associated with outcomes. virological sfts diagnosis with high sensitivity and specificity are required in disease endemic areas. | 2016 | 27045364 |
| tick-borne infections in human and animal population worldwide. | the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ... | 2015 | 27047089 |
| role of parasitic vaccines in integrated control of parasitic diseases in livestock. | parasitic infections adversely affect animal's health and threaten profitable animal production, thus affecting the economy of our country. these infections also play a major role in the spread of zoonotic diseases. parasitic infections cause severe morbidity and mortality in animals especially those affecting the gastrointestinal system and thus affect the economy of livestock owner by decreasing the ability of the farmer to produce economically useful animal products. due to all these reasons ... | 2015 | 27047140 |
| emergence of oriental theileriosis in cattle and its transmission through rhipicephalus (boophilus) microplus in assam, india. | the aim of the present study was to investigate the presence of theileria in blood samples of crossbred and indigenous adult cows raised under unorganized small scale farming system in a babesia and anaplasma endemic geographical area from assam, india and to see its transmission through rhipicephalus (boophilus) microplus ticks. | 2015 | 27047205 |
| atypical challenging and first case report of babesiosis in ecuador. | babesia is known to be prevalent in the eastern united states and other temperate countries but the prevalence of babesia is not well known in the tropical malaria-endemic countries because of the higher prevalence of malaria. a 72-year-old hispanic male from ecuador presenting with increasing left lower quadrant abdominal pain and distention for one year. he experienced nausea, vomiting, diarrhea, fever, chill, and myalgias. he reported 9 kg weight loss over the last two months. patient moved t ... | 2016 | 27051577 |
| extensive polymorphism of ra86 genes in field populations of rhipicephalus appendiculatus from kenya. | commercial vaccines based on recombinant forms of the bm86 tick gut antigen are used to control the southern cattle tick, rhipicephalus microplus, a 1-host species, in australia and latin america. we describe herein sequence polymorphism in genes encoding ra86 homologues of bm86 in the brown ear tick, rhipicephalus appendiculatus, isolated from four kenyan field populations and one laboratory colony. sequencing of 19 ra86 sequences defined two alleles differentiated by indels, encoding 693 amino ... | 2016 | 27051976 |
| cattle experimentally infected by anaplasma marginale: influence of splenectomy on disease pathogenesis, oxidative profile, and antioxidant status. | bovine anaplasmosis is caused by the obligate intraerythrocytic bacteria anaplasma marginale. these bacteria are transmitted by tick species such as rhipicephalus (boophilus) microplus, blood-sucking insects, and fomites (needles, clippers, and other blood contaminated equipment). during the acute phase of infection, animals may develop fever, anemia, jaundice, and hepatosplenomegaly. the aims of this study are to quantify the bacteremia by quantitative pcr in eight naïve calves experimentally i ... | 2016 | 27060744 |
| cloning and molecular analysis of voraxin-α gene of rhipicephalus (boophilus) microplus. | to identify suitable targets for development of cross-protective tick vaccine, in silico analysis was attempted and male tick derived molecule, voraxin-α was targeted. the voraxin-α homologue of rhipicephalus (boophilus) microplus was cloned, sequenced and analyzed employing standard methods. the deduced amino acids sequence analysis of the 419 bp cloned voraxin-α gene of r. (b.) microplus indicated very high (94.6 %) similarity with voraxin-α of the r. appendiculatus and moderate to low identit ... | 2016 | 27065622 |
| emerging status of anaplasmosis in cattle in hisar. | anaplasma marginale is a rickettsial pathogen responsible for progressive anemia in ruminants leading to huge economic losses. the objectives of this cross-sectional study were to determine the prevalence of anaplasmosis and therapeutic evaluation of traditional line of treatment. | 2015 | 27065645 |
| distribution of spotted fever group rickettsiae in hard ticks (ixodida: ixodidae) from panamanian urban and rural environments (2007-2013). | tick-borne rickettsiosis is an important emerging disease in panama; to date, there have been 12 confirmed cases, including eight fatalities. to evaluate the distribution of rickettsiae in panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. a total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. we found the following tick species: rhipicephalus sanguineus s.l. (present in 5 ... | 2016 | 27068930 |
| neurotrophin, p75, and trk signaling module in the developing nervous system of the marine annelid platynereis dumerilii. | in vertebrates, neurotrophic signaling plays an important role in neuronal development, neural circuit formation, and neuronal plasticity, but its evolutionary origin remains obscure. we found and validated nucleotide sequences encoding putative neurotrophic ligands (neurotrophin, nt) and receptors (trk and p75) in two annelids, platynereis dumerilii (errantia) and capitella teleta (sedentaria, for which some sequences were found recently by wilson, 2009). predicted protein sequences and structu ... | 2016 | 27069919 |
| mapping polyclonal antibody responses to bacterial infection using next generation phage display. | mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. in the first instance, pigs experimentally infected with salmonella enterica serovar typhimurium was investigated. igg samples from twelve infected pigs were probed in ... | 2016 | 27072017 |
| contributions to rickettsioses research in colombia (1917-1943), luis b. patiño camargo. | colombian physician luis benigno patiño camargo was one of the pioneers in the study of rickettsioses in south america, demonstrating for the first time in colombia the presence of rickettsia rickettsii as the etiological agent of a highly deadly exanthematic febrile syndrome in the 1930s. however, patiño-camargo performed other investigations from 1917-1943, which represent the first descriptions and scientific evidence of the presence of r. prowazekii and r. typhi in colombia. almost 60 years ... | 2016 | 27074327 |