Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| silver-enhanced colloidal gold metalloimmunoassay for schistosoma japonicum antibody detection. | a silver-enhanced colloidal gold metalloimmunoassay has been proposed for the determination of schistosoma japonicum antibody (sjab) in rabbit serum. the adult worm antigen of s. japonicum (sjag) was adsorbed passively on the walls of a polystyrene microwell and then reacted with the desired sjab. the colloidal gold-labeled goat anti-rabbit igg secondary antibody was adsorbed on the walls of the polystyrene microwells through the reaction with sjab, followed by the silver enhancement process, di ... | 2005 | 16018885 |
| copro-pcr based detection of schistosoma eggs using mitochondrial dna markers. | we report on a sensitive, specific and easy to interpret pcr based diagnostic tool for the detection of schistosoma japonicum eggs in the faeces of infected mammalian hosts. primer pairs were designed to amplify regions of the mitochondrial dna of the parasite. the specificity of the pcr primers was tested using either faecal samples from non-infected hosts or hosts infected with the related schistosome species s. mansoni. sensitivity was investigated in a study, which differentiated the presenc ... | 2005 | 16038793 |
| [boost effect of recombinant il-4 on protection of schistosoma japonicum cathepsin b dna vaccine in mice against the parasite]. | to investigate the enhancement effect of il-4 expression plasmid on cathepsin b dna vaccine of schistosoma japonicum (sj) in mice. | 2005 | 16042165 |
| [protective immunity induced by multivalent dna vaccine of schistosoma japonicum mr23 x 10(3) membrane antigen and il-12 in mice]. | to develop multivalent dna vaccine pv-il12-sj23 which co-expresses sj23 and cytokine il-12, and investigate its protective efficacy in balb/c mice against challenge infection. | 2005 | 16042171 |
| [acquisition and analysis of 149 ests and 18 novel genes of schistosoma japonicum]. | to acquire and analyze adult stage schistosoma japonicum (chinese strain) expressed sequence tags and new genes from an adult s. japonicum cdna library, and to search new vaccine candidates and drug targets. | 2005 | 16042172 |
| [protective immunity of the recombinant schistosoma japonicum specific very low density lipoprotein binding protein as a vaccine candidate]. | to investigate the protective immunity against schistosoma japonicum in mice immunized with recombinant specific very low density lipoprotein binding protein (svlbp) and its potential as vaccine candidate. | 2005 | 16042176 |
| [development and identification of monoclonal antibodies against the recombinant p38 antigen of schistosoma japonicum]. | to express p38 of schistosoma japonicum in escherichia coli bl21 and develop the monoclonal antibodies (mcab) against rsjp38. | 2005 | 16042180 |
| [enhancement of the protective effect of sjc23 dna vaccine against schistosoma japonicum infection by immunostimulatory sequence]. | to investigate the effect of immunostimulatory sequence on sjc23 dna vaccine against schistosoma japonicum infection. | 2005 | 16042196 |
| a potential impact of climate change and water resource development on the transmission of schistosoma japonicum in china. | there is growing consensus among climate modellers that the unusual global warming observed in the last decades of the 20th century is primarily forced by human activities, namely greenhouse gas increases in the atmosphere. global warming will trigger alterations in physical and biological systems, including shifts in the spatio-temporal distribution of disease vectors, but the nature and extent of these changes are poorly understood. the purpose of the present study was to assess the potential ... | 2005 | 16044681 |
| sex differences in the malnourished status of chinese children due to schistosomiasis infections and inadequate dietary intake. | based on nutritional and parasitological examinations of 389 children aged 10-13 years in five primary schools in the schistosomiasis endemic dongting lake region of china, the causal factors of their retarded growth, represented by height, weight, mid-upper arm circumference and body mass index, were investigated. among the four parasites, schistosomiasis infection due to schistosoma japonicum played a significant role, with higher rates in girls than in boys. praziquantel treatment of schistos ... | 2005 | 16077466 |
| a double-blind field trial on the effects of artemether on schistosoma japonicum infection in a highly endemic focus in southern china. | to further strengthen the evidence-base of artemether for the control of schistosomiasis japonica, a randomised controlled trial was carried out in the poyang lake region, a highly endemic area in southern china. a total of 783 individuals, aged 6-60 years, were enrolled. they were first given a single oral dose of praziquantel (50 mg/kg). then, they were randomly assigned oral artemether (6 mg/kg) or placebo, administered once every 2 weeks for 9-11 doses, covering the entire transmission seaso ... | 2005 | 16112071 |
| development of antischistosomal drugs in china, with particular consideration to praziquantel and the artemisinins. | remarkable achievements have been made in the control of schistosomiasis in china, with chemotherapy playing a seminal role. from the early 1950s through the early 1980s, chinese scientists made considerable progress in discovery and development of compounds with antischistosomal properties, including antimonials, non-antimonials and various effective principles stemming from traditional herbs. however, only few compounds entered clinical testing, while others were abandoned mainly due to their ... | 2005 | 16112072 |
| the role of 'passive chemotherapy' plus health education for schistosomiasis control in china during maintenance and consolidation phase. | in order to explore the possibility of further optimising schistosomiasis control during the maintenance and consolidation phase in china, two highly endemic villages were selected to compare the strategy of 'passive chemotherapy' plus health education to that of mass chemotherapy singly. emphasis was placed on treatment coverage with praziquantel among individuals infected with schistosoma japonicum and costs incurred for treating an infected person. the results show that the former strategy wa ... | 2005 | 16112637 |
| a review of geographic information system and remote sensing with applications to the epidemiology and control of schistosomiasis in china. | geographic information system (gis) and remote sensing (rs) technologies offer new opportunities for rapid assessment of endemic areas, provision of reliable estimates of populations at risk, prediction of disease distributions in areas that lack baseline data and are difficult to access, and guidance of intervention strategies, so that scarce resources can be allocated in a cost-effective manner. here, we focus on the epidemiology and control of schistosomiasis in china and review gis and rs ap ... | 2005 | 16112638 |
| the public health significance and control of schistosomiasis in china--then and now. | the description of schistosomiasis in china dates back more than two millennia. the disease caused social and economic hardship, and the rates of morbidity and mortality were high. in the mid 1950s, when china's population was approximately 600 million, an estimated 11.6 million people were infected with schistosoma japonicum. hence, a national control programme was launched, with an emphasis on intermediate host snail control by means of environmental management. over the past 50 years, the nat ... | 2005 | 16125655 |
| use of praziquantel for clinical treatment and morbidity control of schistosomiasis japonica in china: a review of 30 years' experience. | chemotherapy is widely acknowledged as the most important, rapid and cost-effective method of reducing morbidity due to schistosome infections. the discovery of praziquantel in the 1970s has been a breakthrough for treatment of patients infected with schistosomes, including schistosoma japonicum in china, and entire communities at risk of schistosomiasis. praziquantel is usually administered in a single oral dose and has no or only mild and transient side effects. the drug is highly efficacious ... | 2005 | 16125657 |
| immunity to schistosomiasis in animals: an update. | the present paper reviews the available literature on the development of immunity to animal schistosoma infections. the majority of the studies on animal schistosomiasis were performed in cattle and pigs and only schistosoma mattheei, s. bovis and s. japonicum received particular attention, mainly because of their recognized veterinary significance or zoonotic aspect. although it is an accepted fact that acquired resistance to schistosoma is of major importance in the regulation of infection int ... | 2005 | 16138850 |
| prospects for development of a transmission blocking vaccine against schistosoma japonicum. | despite intensive long-term control programmes, schistosomiasis japonica remains a serious public health problem in china and the philippines. the termination of mass praziquantel-treatment has seen a dramatic recent rebound in both its prevalence and associated morbidity. schistosomiasis japonica is a zoonosis but, despite complicating control efforts, this feature provides a practical method for attacking schistosoma japonicum through development and deployment of a transmission blocking veter ... | 2005 | 16138851 |
| a geographic information and remote sensing based model for prediction of oncomelania hupensis habitats in the poyang lake area, china. | a model was developed using remote sensing and geographic information system technologies for habitat identification of oncomelania hupensis, the intermediate host snail of schistosoma japonicum, in the poyang lake area, china. in a first step, two multi-temporal landsat tm 5 satellite images, one from the wet and the second from the dry season, were visually classified into different land-use types. next, the normalized difference vegetation index was extracted from the images and the tasseled- ... | 2005 | 16140246 |
| application of dipstick dye immunoassay (ddia) kit for the diagnosis of schistosomiasis mekongi. | the dipstick dye immunoassay (ddia), developed in china for the detection of antibodies against schistosoma japonicum, relies on soluble egg antigen (sea) labelled with a colloidal dye. this assay is not only rapid, simple and inexpensive, but also particularly useful for screening in the field. in order to determine whether s. japonicum antigens are sufficiently cross-reactive to make the assay applicable for the diagnosis also of s. mekongi a ddia approach based on the s. japonicum sea was tri ... | 2005 | 16143289 |
| [preliminary studies on immunoprotection in mice after vaccination with schistisoma japonicum cercaria cells]. | 2004 | 16145927 | |
| remote sensing and spatial statistical analysis to predict the distribution of oncomelania hupensis in the marshlands of china. | remote sensing and spatial statistical analysis were employed to predict the distribution of oncomelania hupensis, the intermediate host snail of schistosoma japonicum, in the marshlands of jiangning county in china. surrogate indices related to environmental factors in the marshlands were derived from a landsat 7 etm+ image, and the relationship between environmental covariates and the density of o. hupensis was analyzed by stepwise regression models and ordinary kriging. although stepwise regr ... | 2005 | 16150415 |
| the role of health education and health promotion in the control of schistosomiasis: experiences from a 12-year intervention study in the poyang lake area. | the objectives of this study were to examine the short-term effects of health education and health promotion in the control of schistosomiasis, and to monitor the long-term impact on re-infection patterns. the study was carried out in six schistosoma japonicum-endemic villages located in the poyang lake area. three different interventions were implemented, namely (i) health education by means of video tapes, training in prevention of infection, and a 'rewards/punishment' programme for schoolchil ... | 2005 | 16154103 |
| an economic evaluation of the national schistosomiasis control programme in china from 1992 to 2000. | the world bank loan project, by far the largest effort in china for schistosomiasis control since control activities were initiated in the mid 1950s, was carried out for a 9-year period commencing in 1992 in the 8 provinces where schistosoma japonicum remained endemic when the project started. to evaluate its impact, a retrospective economic evaluation was done in 2001. six representative counties, i.e. huarong in hunan province, qianjiang in hubei province, yugan in jiangxi province, tongling i ... | 2005 | 16154104 |
| transmission control of schistosomiasis japonica: implementation and evaluation of different snail control interventions. | the great progress made in the control of schistosomiasis japonica in china is to some extent explained by successful intermediate host snail control, in particular with environmental management commencing some 50 years ago. by 1995, interruption of schistosoma japonicum transmission had been achieved in five of the 12 schistosome-endemic provinces while endemic areas in the remaining provinces had been reduced significantly, and snail habitats had decreased by 74%. in this paper, we review the ... | 2005 | 16154105 |
| development of a vaccine against schistosoma japonicum in china: a review. | significant progress has been made over the past 50 years in the control of schistosomiasis japonica in china. however, recent data suggest that the disease is re-emerging. by the end of 2003, schistosoma japonicum was still endemic in 110 counties in seven provinces in the southern part of china where the long-term reduction of the disease has been replaced by an increase in the number of people infected and areas infested by the intermediate host snail, i.e. oncomelania hupensis. explanations ... | 2005 | 16168945 |
| development of a novel pcr assay capable of detecting a single schistosoma japonicum cercaria recovered from oncomelania hupensis. | location and time-specific variability in schistosoma japonicum cercarial density has been shown to be high in the mountainous regions of sichuan province, china. a polymerase chain reaction (pcr) assay for the detection of schistosome cercariae in these environments would aid in the determination of environmental risk, and the identification of individual-level risk factors. here the authors present a highly sensitive and specific pcr assay for the detection of s. japonicum cercariae in laborat ... | 2005 | 16174414 |
| field evaluation of a rapid, visually-read colloidal dye immunofiltration assay for schistosoma japonicum for screening in areas of low transmission. | to determine the validity of a recently developed rapid test--a colloidal dye immunofiltration assay (cdifa)--used by health workers in field settings to identify villagers infected with schistosoma japonicum. | 2005 | 16175827 |
| transmission of schistosoma japonicum by humans and domestic animals in the yangtze river valley, anhui province, china. | the aim of the present work was to assess the relative contribution to transmission of schistosoma japonicum by humans and domestic animals in two villages in the yangtze river valley in anhui province, china. a cross-sectional survey was conducted to determine the prevalence and intensity of s. japonicum in humans, cattle, water buffaloes, horses, pigs, goats, dogs and cats. additionally, for each host species the number of individuals and the mean faecal excretion per day was determined. resul ... | 2005 | 16188215 |
| determination of serum levels of ykl-40 and hyaluronic acid in patients with hepatic fibrosis due to schistosomiasis japonica and appraisal of their clinical value. | the objectives of this study were (i) to evaluate the serum levels of human cartilage glycoprotein 39 (ykl-40) and hyaluronic acid (ha) in people infected with schistosoma japonicum, and (ii) to determine their clinical value. a total of 563 people were subjected to ultrasonography, and 60 patients were identified with either mild (n=30) or severe (n=30) hepatic fibrosis. in addition, 28 healthy subjects were included as controls. blood sera of these 88 people were examined with regard to the le ... | 2005 | 16188216 |
| estimating sensitivity and specificity of a faecal examination method for schistosoma japonicum infection in cats, dogs, water buffaloes, pigs, and rats in western samar and sorsogon provinces, the philippines. | schistosoma japonicum causes a chronic parasitic disease, which persists as a major public health concern in the philippines, the people's republic of china and indonesia. this infection is unique among helminthic zoonoses because it can infect humans and more than 40 other mammals. the objective of this study was to estimate the sensitivity and specificity of the danish bilharziasis laboratory technique in cats, dogs, pigs, water buffaloes and rats in the philippines. faecal samples from each a ... | 2005 | 16188261 |
| surveillance and control of post-transmission schistosomiasis in jiaxing prefecture, zhejiang province, china. | a surveillance system has been put in place in the zhejiang province since achieving there the criteria for transmission interruption of schistosomiasis japonica. suspected patients and special high-risk groups (e.g. the so called 'floating population' and children under 14 years of age) are screened for schistosoma japonicum using serological tests. those with positive serological result are subjected to faecal examination and if s. japonicum eggs are found they are treated with praziquantel an ... | 2005 | 16198300 |
| surveillance of schistosomiasis in five provinces of china which have reached the national criteria for elimination of the disease. | surveillance of schistosomiasis consists mainly of surveys carried out in formerly endemic areas to observe infection and re-infection of resident and mobile human populations, and domestic animals (e.g. bovines) plus snail monitoring. here, we focus on the schistosomiasis situation in the five chinese provinces which have reached the national criteria for elimination of the disease, i.e. fujian, guangdong, guangxi, shanghai and zhejiang. with the exception of three newly infected persons in gua ... | 2005 | 16198301 |
| the social and economic context and determinants of schistosomiasis japonica. | a variety of biological and social factors govern schistosomiasis japonica in china. social factors include those at a national and regional level, such as policies and patterns of development, which impact on local economic activities, and affect community, household and personal risk factors of infection. drawing on research conducted in china, we illustrate how social structural and related factors influence individual risk and prevalence of infection. at a macro-level, political changes have ... | 2005 | 16202596 |
| surveillance for schistosomiasis japonica in china from 2000 to 2003. | according to the protocol of the national surveillance project, longitudinal observation of the endemic situation of schistosomiasis japonica in china has been carried out at 20 sentinel surveillance sites, commencing in 2000. after four years of surveillance, the infection rate of schistosoma japonicum gradually decreased in seven sentinel sites. in 10 sites, infection rates remained relatively stable over time, while there was a marked increase in one site. no clear trend became apparent in th ... | 2005 | 16202597 |
| evaluation on the applied value of the dot immunogold filtration assay (digfa) for rapid detection of anti-schistosoma japonicum antibody. | the dot immunogold filtration assay (digfa) is a rapid technique for the detection of anti-schistosoma japonicum antibody. its sensitivity with regard to sera obtained from patients with acute or chronic schistosomiasis was shown to be 100 and 96.9%, respectively. the specificity when using sera of people living in an area non-endemic for schistosomiasis japonica was 100%. cross-reaction rates for paragonimiasis and clonorchiasis patients were 14.3% and 0%, respectively. parallel serum tests of ... | 2005 | 16207482 |
| identification and characterization of peptides mimicking the epitopes of metalloprotease of schistosoma japonicum. | in an attempt to isolate and characterize peptides mimicking epitopes of metalloprotease and explore their immunological protection against schistosoma japonicum (s. japonicum), polyclonal anti-metalloprotease sera was prepared to screen a 12-mer random peptide library to isolate phages binding specially to antisera igg. then, phage elisa, animal immunization, dna sequencing, western blotting and enzymatic activity neutralizing analysis were used to characterize the selected phage clones. all of ... | 2005 | 16212890 |
| schistosoma japonicum: a method for transformation by electroporation. | despite of our knowledge of genetic make up of schistosomes, a number of genes have not been characterized largely due to lack of effective transformation protocols. here we present electroporation as a strategy for effective introduction of plasmids dna into schistosomula and adults. using plasmids of pegfp-c1 as an expression vector, we first verified that the cmv promoter could direct egfp to express in primary culture cells from schistosoma japonicum. subsequently, the plasmids were introduc ... | 2005 | 16212956 |
| modeling the dynamics and control of schistosoma japonicum transmission on bohol island, the philippines. | we have investigated a mathematical model for the transmission of schistosoma japonicum in the infested region of northeastern bohol island in the philippines. the development of transmission models is important for planning control strategies. since s. japonicum has a complicated mode of transmission, the rates of transmission among its hosts cannot be measured directly by field observation. instead, they have been estimated through model analysis. the model takes into account the seasonal vari ... | 2006 | 16213188 |
| determination of the period for establishment of a liver network echogenic pattern in schistosoma japonicum infection. | schistosomiasis is caused by infection with schistosoma haematobium, s. mansoni, s. japonicum, or s. mekongi. s. japonicum infection results in liver cirrhosis at the final stage. a "network" (nw) echogenic pattern on hepatic ultrasonography appears to be specific to s. japonicum infection. the principal aim of the present study was to determine the exact year(s) or even month(s) required for the establishment of the liver nw echogenic pattern from the initial infection in young patients with sc ... | 2006 | 16216544 |
| population genetics of multi-host parasites--the case for molecular epidemiological studies of schistosoma japonicum using larval stages from naturally infected hosts. | population genetics of multi-host pathogens offers great potential for the understanding of their complex epidemiology but care must be taken to ensure that the sampling procedure does not bias estimates of population indices. the transfer of material to laboratory passage, in particular, runs the risk of bottlenecking and imposing non-random host-induced selection pressures according to the hosts used in passage. we present a novel technique allowing single-locus microsatellite genotyping of th ... | 2005 | 16255820 |
| helicobacter pylori and schistosoma japonicum co-infection in a chinese population: helminth infection alters humoral responses to h. pylori and serum pepsinogen i/ii ratio. | the effects of helminth infection on humoral igg responses and clinical outcome of gastric helicobacter pylori infection are unknown. igg and igg subclass responses to h. pylori and serum pepsinogen i/ii ratio, a marker of gastric atrophy, were investigated in a schistosoma japonicum prevalent chinese population. h. pylori, caga and igg subclass responses were assayed by elisa. serum pepsinogen i and pepsinogen ii were assayed by elisa and the pepsinogen i/ii ratio determined. in 150 subjects, i ... | 2006 | 16260169 |
| identification of immunodominant th1-type t cell epitopes from schistosoma japonicum 28 kda glutathione-s-transferase, a vaccine candidate. | th1-type cytokines produced by the stimulation of th1-type epitopes derived from defined schistosome-associated antigens are correlated with the development of resistance to the parasite infection. schistosoma mansoni 28 kda glutathione-s-transferase (sm28gst), a major detoxification enzyme, has been recognized as a vaccine candidate and a phase ii clinical trial has been carried out. sheep immunized with recombinant schistosoma japonicum 28gst (sj28gst) have shown immune protection against the ... | 2005 | 16270154 |
| design of potent inhibitors for schistosoma japonica glutathione s-transferase. | we implemented both structure-based drug design and the concept of polyvalency to discover a series of potent and unsymmetrical schistosoma japonicum glutathione s-transferase (sjgst) inhibitors 10-12. this strategy achieved not only an excellent enhancement (10- to 490-fold) in the inhibitory potency, compared to the monofunctional analogues 1-5, but was also an effective modification by selecting a hydrophobic moiety with a flexible linker. the designed compounds with a low micromolar hit demo ... | 2006 | 16275109 |
| functional significance of low-intensity polyparasite helminth infections in anemia. | we wanted to quantify the impact that polyparasite infections, including multiple concurrent low-intensity infections, have on anemia. | 2005 | 16288383 |
| [screening of a hepatic stellate cells subtracted cdna library of differentially expressed genes in mice with schistosomiasis japonica]. | to construct and screen a hepatic stellate cells(hscs) subtracted cdna library, in order to seek differentially expressed genes in mice infected with schistosoma japonicum. | 2005 | 16296601 |
| [transformation of schistosomulae by electroporation and transient expression of the enhanced green fluorescent protein (egfp) gene]. | to explore the possibility of heterogeneous gene to express in juvenile schistosoma japonicum and the application of electroporation in transformation of schistosomulae. | 2005 | 16296605 |
| [preparation and preliminary application of monoclonal antibodies against adult worm of angiostrongylus cantonensis]. | to develop and identify monoclonal antibodies (mcabs) against adult worm of angiostrongylus cantonensis and observe its applicability. | 2005 | 16296607 |
| synthesis of complex-type glycans derived from parasitic helminths. | chemical synthesis of complex-type glycans 1 and 2 derived from eggs of parasitic helminths, schistosoma mansoni and schistosoma japonicum, is described. these branched sugar chains were synthesized regio- and stereoselectively by using beta-mannosylation, desilylation under high pressure, and glycosylation in frozen solvent as key transformations. | 2006 | 16297623 |
| [the adjuvant effect of il-12 on protective immunity of schistosoma japonicum fatty acid binding protein (sj14fabp)]. | to study the immune protection of schistosoma japonicum fatty acid binding protein (sj14fabp) dna vaccine enhanced by il-12. | 2005 | 16300005 |
| [fusion expression and antigenicity analysis of miracidial antigen from eggs of schistosoma japonicum]. | to express the miracidial antigen from eggs of schistosoma japonicum (chinese mainland strain) (sjmp10), and investigate the role of the miracidial antigen during the hepatic granuloma formation of schistosomiasis. | 2005 | 16300006 |
| conquering schistosomiasis in china: the long march. | the last half-century of schistosomiasis control activities in china have brought down the overall prevalence of human infection with schistosoma japonicum to less than 10% of the level initially documented in the mid 1950s. importantly, this reduction is not only, or even mainly, due to the advent of praziquantel in the 1970s and its subsequent dramatic fall in price. instead, it is the result of a sustained, multifaceted national strategy, adapted to different eco-epidemiological settings, whi ... | 2005 | 16312039 |
| familial aggregation of human infection with schistosoma japonicum in the poyang lake region, china. | despite the success of extensive control measures that have been implemented in china for over 50 years, the number of individuals infected with schistosoma japonicum remains high in the existing endemic areas. a variance components analysis was undertaken to estimate the heritable and environmental components that contribute to s. japonicum infection in the poyang lake region of jiangxi province, pr china. the total target population was 3148 from four separate administrative villages. two thou ... | 2006 | 16321389 |
| production of a truncated soluble human semicarbazide-sensitive amine oxidase mediated by a gst-fusion protein secreted from hek293 cells. | elevated levels of semicarbazide-sensitive amine oxidase (ssao) activity have been observed in several human conditions such as congestive heart failure, diabetes mellitus, and inflammation. the reactive aldehydes and hydrogen peroxide produced by ssao have been suggested to contribute to the progression of vascular complications associated with these conditions. in addition, ssao activity has been shown to be involved in the leukocyte extravasation process at sites of inflammation. to facilitat ... | 2006 | 16325418 |
| phosphorylation of glutathione-s-transferase by protein kinase c-alpha implications for affinity-tag purification. | expression and purification of proteins as fusions with glutathione s-transferase (gst) is a standard and widely employed system. in more than 2,500 published studies, gst has been used to facilitate the purification of recombinant proteins, assess protein-protein interactions, and establish protein function. in this report, we provide evidence that gst can be phosphorylated in vitro by protein kinase c-alpha (pkc-alpha) at ser-93. therefore, since gst itself may be a target for a number of cata ... | 2005 | 16328982 |
| system level metabolic effects of a schistosoma japonicum infection in the syrian hamster. | a metabolic profiling strategy was used to investigate the metabolic responses of syrian hamsters (slac) to a schistosoma japonicum infection using high resolution 1h nuclear magnetic resonance (nmr) spectroscopy and pattern recognition. in two independent experiments, male hamsters were each infected with 100 s. japonicum cercariae. at days 34-36 post-infection, urine was obtained from hamsters housed individually in metabolism cages. at the same time, urine was collected from age- and sex-matc ... | 2006 | 16337285 |
| genetic and household risk factors for schistosoma japonicum infection in the presence of larger scale environmental differences in the mountainous transmission areas of china. | schistosoma japonicum egg excretion and kinship relationship data from 13 endemic villages in the mountainous transmission area near xichang, in sichuan province, china, were analyzed via a variance components methodology to assess the relative contribution of kinship, shared household, and shared village to the risk of infection. large intervillage differences in egg counts exist in this region due to differences in transmission potential related to environmental differences in snail density an ... | 2005 | 16354828 |
| nutritional status improves after treatment of schistosoma japonicum-infected children and adolescents. | studies addressing the relation between chronic schistosomiasis japonica and nutritional status are limited. we conducted a longitudinal treatment-reinfection study in leyte, the philippines, among 477 schistosoma japonicum-infected subjects aged 7-20 y, to evaluate changes in nutritional status after treatment with praziquantel. stool, tanner stage, anthropometric indices, and hemoglobin (hb) were evaluated at baseline, 4 wk post-treatment, and every 3 mo for 18 mo. height-for-age z-score (haz) ... | 2006 | 16365080 |
| t-helper-2 cytokine responses to sj97 predict resistance to reinfection with schistosoma japonicum. | although schistosomiasis is effectively treated with praziquantel, rapid reinfection with rebound morbidity precludes effective control based on chemotherapy alone and justifies current efforts to develop vaccines for these parasites. using a longitudinal treatment-reinfection study design with 616 participants 7 to 30 years of age, we evaluated the relationship between cytokine responses to schistosoma japonicum soluble adult worm extract (swap), sj97, sj22.6, and sj67, measured 4 weeks after t ... | 2006 | 16368992 |
| the effect of a mutagen (n-methyl-n-nitro-n-nitrosoguanidine) on cultured cells from adult schistosoma japonicum. | the transforming effect of n-methyl-n-nitro-n-nitrosoguanidine (mnng) on cultured cells from schistosoma japonicum (s. japonicum) was studied using mono-factor and orthogonal tests. under the influence of mnng, cultured cells grew well, and cell survival time was more than 246 days in low-serum medium. when treated with 3 mug/ml mnng for 48 h, the number of dividing cells increased significantly as determined by bright-field and scanning electron microscopy (sem). under these conditions, abundan ... | 2006 | 16385406 |
| schistosoma japonicum triose-phosphate isomerase plasmid dna vaccine protects pigs against challenge infection. | the protective efficacy of a schistosoma japonicum, chinese strain, triose-phosphate isomerase (tpi) plasmid dna vaccine was examined in naïve pigs. pigs were vaccinated with the tpi dna-plasmid alone, or in conjunction with il-12 as pcdna3.1-p35, pcdna3.1-p40 plasmids via intramuscular injection. control pigs were immunized with equivalent amounts of pcdna3.1. pigs were immunized 3 times at 21-day intervals and challenged 30 days after the final boost. forty-five days post-challenge, pigs were ... | 2006 | 16393355 |
| investigation on the epidemiological factors of clonorchis sinensis infection in an area of south china. | to detect the epidemiological factors of clonorchis sinensis infection in hengxian county, one of counties in guangxi zhuang autonomous region where schistosoma japonicum was endemic but eliminated in the late 1980s, a questionnaire was designed with 37 questions covering socioeconomic conditions and human behavior, contamination of the environment and fish ponds, inadequate farming/fishery practices; and the formalin-ether sedimentation technique was used to examine the feces of cats, dogs and ... | 2005 | 16438134 |
| an amplified mass piezoelectric immunosensor for schistosoma japonicum. | an ultrasensitive piezoelectric immunosensor using an amplification path based on an insoluble biocatalyzed precipitation product has proposed for schistosoma japonicum. a mercapto schistosoma japonicum antigen was self-assembled onto the quartz crystal surface via an au nanoparticle mediator monolayer to sense the schistosoma japonicum antibody (sjab). and the horseradish peroxidase labeled protein a conjugate which was bounded to the sjab by a "sandwich" format was used as a biocatalyst for th ... | 2006 | 16439107 |
| investigation of recombinant schistosoma japonicum paramyosin fragments for immunogenicity and vaccine efficacy in mice. | schistosoma japonicum paramyosin, a 97 kda myofibrillar protein, is a recognized vaccine candidate against schistosomiasis. to improve its expression and to identify protective epitopic regions on paramyosin, the published chinese schistosoma japonicum paramyosin cdna sequence was redesigned using pichia codon usage and divided into four overlapping fragments (fragments 1, 2, 3, 4) of 747, 651, 669 and 678 bp, respectively. these gene fragments were synthesized and expressed in pichia pastoris ( ... | 2006 | 16441505 |
| association between serum cytokine profiles and schistosomiasis-related hepatic fibrosis: infection by schistosoma japonicum versus s. mansoni. | 2006 | 16453272 | |
| an historical and genomic view of schistosome conjugal biology with emphasis on sex-specific gene expression. | the genetic programmes associated with the sexual biology of dioecious schistosomes remain a critically important but significantly understudied area of parasitology. throughout the last four decades, progress has been slow in describing the gross antigenic and proteomic differences linked to sexually mature schistosomes and in characterizing some of the sex-associated transcripts and regulatory mechanisms induced during developmental maturation. these investigations have been severely hindered ... | 2004 | 16454894 |
| the complete mitochondrial genomes of schistosoma haematobium and schistosoma spindale and the evolutionary history of mitochondrial genome changes among parasitic flatworms. | complete mitochondrial genome sequences for the schistosomes schistosoma haematobium and schistosoma. spindale have been characterized. s. haematobium is the causative agent of urinary schistosomiasis in humans and s. spindale uses ruminants as its definitive host; both are transmitted by freshwater snail intermediate hosts. results confirm a major gene order rearrangement among schistosomes in all traditional schistosoma species groups other than schistosoma japonicum; i.e., species groups s. m ... | 2006 | 16464618 |
| schistosomiasis japonica, anemia, and iron status in children, adolescents, and young adults in leyte, philippines 1. | observational and interventional evidence supports a relation between human schistosomiasis and anemia; however, the exact causal mechanisms remain unclear. eggs translocating across the intestinal or bladder wall may result in extracorporeal blood loss with subsequent iron deficiency. alternatively, anemia may result from cytokine-mediated dyserythropoiesis, as seen in anemia of inflammation. | 2006 | 16469997 |
| a drug-based intervention study on the importance of buffaloes for human schistosoma japonicum infection around poyang lake, people's republic of china. | schistosomiasis japonica is a zoonosis of major public health importance in southern china. we undertook a drug intervention to test the hypothesis that buffalo are major reservoirs for human infection in the marshlands/lake areas, where one million people are infected. we compared human and buffalo infection rates and intensity in an intervention village (jishan), where humans and buffalo were treated with praziquantel, and a control village (hexi), where only humans were treated, in the poyang ... | 2006 | 16474093 |
| establishment of a gis monitoring system for schistosomiasis japonica in kofu, japan. | although the incidence of human infection with schistosoma japonicum in japan fell to zero in 1977, the threat of the possible re-emergence of the disease caused by this trematode still exists. surveillance of the parasite's intermediate host, oncomelania nosophora, in kofu basin therefore began in 1996. a simple, new method for monitoring o. nosophora in an at-risk area in kofu, which is based on a geographical information system (gis), was established. at each monitoring site (of which there w ... | 2006 | 16492362 |
| "zero-length" cross-linking in solid state as an approach for analysis of protein-protein interactions. | we have developed a new approach for the analysis of interacting interfaces in protein complexes and protein quaternary structure based on cross-linking in the solid state. protein complexes are freeze-dried under vacuum, and cross-links are introduced in the solid phase by dehydrating the protein in a nonaqueous solvent creating peptide bonds between amino and carboxyl groups of the interacting peptides. cross-linked proteins are digested into peptides with trypsin in both h2(16)o and h(2)18o a ... | 2006 | 16501223 |
| re-emerging schistosomiasis in hilly and mountainous areas of sichuan, china. | despite great strides in schistosomiasis control over the past several decades in sichuan province, china the disease has re-emerged in areas where it was previously controlled. we reviewed historical records and found that schistosomiasis had re-emerged in eight counties by the end of 2004 - seven of 21 counties with transmission control and one of 25 with transmission interruption as reported in 2001 were confirmed to have local disease transmission. the average "return time" (from control to ... | 2006 | 16501732 |
| x-ray structure of glutathione s-transferase from schistosoma japonicum in a new crystal form reveals flexibility of the substrate-binding site. | the crystal structure of the 26 kda glutathione s-transferase from schistosoma japonicum (sjgst) was determined at 3 a resolution in the new space group p2(1)2(1)2(1). the structure of orthorhombic sjgst reveals unique features of the ligand-binding site and dimer interface when compared with previously reported structures. sjgst is recognized as the major detoxification enzyme of s. japonicum, a pathogenic helminth causing schistosomiasis. as resistance against the established inhibitor of sjgs ... | 2005 | 16511012 |
| structure of botulinum neurotoxin type d light chain at 1.65 a resolution: repercussions for vamp-2 substrate specificity. | the seven serotypes (a-g) of botulinum neurotoxins (bonts) function through their proteolytic cleavage of one of three proteins (snap-25, syntaxin, and vamp) that form the snare complex required for synaptic vesicle fusion. the different bonts have very specific protease recognition requirements, between 15 and 50 amino acids in length depending on the serotype. however, the structural details involved in substrate recognition remain largely unknown. here is reported the 1.65 a resolution crysta ... | 2006 | 16519520 |
| crystal structure of non-fused glutathione s-transferase from schistosoma japonicum in complex with glutathione. | schistosoma japonicum glutathione s-transferase (sjgst) is a common fusion tag in recombinant protein production, and its 3-dimensional structure has been studied in the context of drug design. we have determined the crystal structure of non-fused sjgst complexed with glutathione, and compare it to complexes between glutathione and sjgst fusion proteins. | 2005 | 16522189 |
| a cross-sectional study of the prevalence of intensity of infection with schistosoma japonicum in 50 irrigated and rain-fed villages in samar province, the philippines. | few studies have described heterogeneity in schistosoma japonicum infection intensity, and none were done in philippines. the purpose of this report is to describe the village-to-village variation in the prevalence of two levels of infection intensity across 50 villages of samar province, the philippines. | 2006 | 16526960 |
| schistosoma japonicum and s. mansoni cercariae: different effects of protein in medium, of mechanical stress, and of an intact complement system on in vitro transformation to schistosomula. | the cercariae of schistosoma japonicum were subjected in vitro to treatments known for schistosoma mansoni to generate schistosomula-like organisms. as a technical prerequisite to pipette or to otherwise handle the sticky cercariae of s. japonicum, the addition of protein to water or medium was found to abolish the stickiness of cercariae of this species. shearing forces exerted in vitro by syringe (22 g) passage are known since long to fully transform s. mansoni cercariae, but this treatment wa ... | 2006 | 16547731 |
| [immunodiagnosis of schistosoma japonicum infection in china: review and prospects]. | 2005 | 16562462 | |
| [immunological characteristics induced in mice by nucleic acid vaccine harboring the gene encoding gapdh in schistosoma japonicum]. | to study the immunological characteristics induced in c57bl/6 mice by nucleic acid vaccine harboring the gene encoding glyceraldehyde-3-phosphate dehydrogenase (gapdh) of schistosoma japonicum. | 2005 | 16562475 |
| [relationship of the intensity of schistosoma japonicum infection to the variation in individual egg count]. | to investigate the relationship of the intensity of schistosoma japonicum infection with the variation in individual egg counts. | 2005 | 16562476 |
| [studies on effect enhancement of the schistosoma japonicum dna vaccine pviv02-il12-sj23 by vegetal polysaccharides]. | to enhance the immunogenicity of the recombinant pvivo2-il12-sj23 vaccine of schistosoma japonicum by using mixed vegetal polysaccharides as adjuvant. | 2005 | 16566205 |
| [in vitro killing activity of the fractional proteins from microtus fortis serum to schistosoma japonicum juveniles]. | to explore the killing effect of different fractional proteins from microtus fortis (mf) serum to s. japonicum juveniles, and to find possible association of the proteins with the natural resistance to schistosome infection. | 2005 | 16566206 |
| [expression of hepatic bcl-2 and bax proteins in schistosome-infected mice and the role of pentoxifylline]. | to study the expression of hepatic bcl-2 and bax proteins in mice infected with schistosoma japonicum and the role of pentoxifylline (ptx) in the expression. | 2005 | 16566217 |
| [development of the specific antibodies and diagnostic antigens of schistosoma japonicum]. | 2005 | 16566226 | |
| surveillance colonoscopy should be conducted in patients with colorectal shistosomiasis even after successful treatment of the disease. | 2006 | 16569365 | |
| transcriptome profiling of lung schistosomula,in vitro cultured schistosomula and adult schistosoma japonicum. | the schistosomulum is the main target of vaccine-induced protective immunity; however, most studies have utilized schistosomula produced by mechanical transformation of infective larvae followed by in vitro culture rather than larvae isolated directly from the lungs of infected mammals. using transmission electron microscopy, we demonstrated that there was little difference in the ultrastructure of schistosoma japonicum schistosomula obtained by the two methods. however, significant differences ... | 2006 | 16570121 |
| [studies on phage displayed mimotopes of a protective monoclonal antibody (ssj14) against schistosoma japonicum]. | to obtain peptides mimicking epitope of a protective mcab ssjl4 specific to schistosoma japonicum and investigate their immuno-protection effects. a phage random 12 peptide library was screened using purified mcab ssj14, 33 clones were picked up for specificity identification by elisa. the epitope of each positive clones were detected by the sequencing analysis technique. the antigenicity of three positive clones (p1, p2 and p11) and their mixture cock-tail were further confirmed by western-blot ... | 2006 | 16572851 |
| molecular cloning and characterization of schistosoma mansoni ftz-f1 interacting protein-1 (smfip-1), a novel corepressor of the nuclear receptor smftz-f1. | in order to characterize protein cofactors of the schistosoma mansoni nuclear receptor smftz-f1, we have screened a yeast two-hybrid adult worm cdna library using a construct expressing the d, e and f domains of smftz-f1 as bait. one of the selected clones encoded a sequence without homologues in any other species, apart from schistosoma japonicum. the complete sequence was obtained by 5' and 3' rapid amplification of cdna ends (race) and comprised 3660 nucleotides with an open reading frame of ... | 2006 | 16574255 |
| long stretches of sequential and identical serine or alanine codons are compatible with an efficient full-length protein expression in escherichia coli. | the schistosoma japonicum glutathione s-transferase (gst) recombinant cdnas, carrying blocks of sequential and identical triplets, consisting of 15-30-45 gct (ala) codons or 15-30 and also up to 75 agc (ser) codons, are expressed efficiently in an escherichia coli system in the form of full-length protein chains, as detected by coomassie-stained sds-polyacrylamide gels, and soluble fusion proteins are purified by gsh-affinity chromatography. high expression levels and high yields of purified rec ... | 2006 | 16600623 |
| development of the rapid and simple elisa (whole blood-elisa) using coconut water-tween as a wash solution for whole blood sample from schistosoma japonicum-infected rabbit and human. | pbs-tween as a wash solution, prepared with distilled water, is used in elisa. in areas where schistosomiasis is endemic, however, distilled water is hard to come by. we have modified a whole blood-elisa test to use coconut water-tween as a wash solution, because coconut water is easy to come by and cheap in the tropics. we applied the test to whole blood samples from rabbits and humans infected with schistosoma japonicum. this modified whole blood-elisa was confirmed to be a rapid, simple, and ... | 2005 | 16610638 |
| phenotypical characterization of intestinal schistosoma japonicum granulomas in pigs. | the pig has been proposed as a model for human schistosomiasis japonica and the use of this animal model is increasing. the inflammatory response to schistosome infection in the liver and intestine of the pig shows morphological differences, and only the hepatic granulomas have been phenotypically characterized. the aim of the present study was to phenotypically characterize the cellular inflammatory response in the cecum by immunohistochemistry with particular reference to perioval granulomatou ... | 2005 | 16610640 |
| recombinant tegumental protein shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against schistosoma japonicum. | a polyhistidine-tagged recombinant tegumental protein schistosoma japonicum very lowdensity lipoprotein binding protein (svlbp) from adult schistosoma japonicum was expressed in escherichia coli. the affinity purified rsvlbp was used to vaccinate mice. the worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.5% and 47.6% less than that from control mice, respectively (p<0.05). there was also a marked increase in the antibody response in vaccinated mic ... | 2006 | 16612506 |
| new perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of schistosoma japonicum. | schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. here we isolated ~ 8,400 potential protein-encoding cdna contigs from schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. in tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tiss ... | 2006 | 16617374 |
| schistosomiasis and neoplasia. | schistosomiasis is endemic in at least 75 tropical and subtropical countries where 600 million people are at risk of which over 200 million are infected. three species, s. hematobium, s. mansoni and s. japonicum, account for the majority of human infections. there is sufficient evidence that s. hematobium, the predominant etiologic agent for urinary schistosomiasis, is carcinogenic to humans leading to squamous cell carcinoma of the urinary bladder, a relatively uncommon vesical cancer in nonend ... | 2006 | 16627960 |
| kinetic study on the irreversible thermal denaturation of schistosoma japonicum glutathione s-transferase. | the thermal unfolding pathway of the schistosoma japonicum glutathione s-transferase (sj26gst) was previously interpreted by applying equilibrium thermodynamics and a reversible two-state model (kaplan et al., (1997) protein science, 6, 399-406), though weak support for this interpretation was provided. in our study, thermal denaturation of sj26gst has been re-examined by differential scanning calorimetry in the ph range of 6.5-8.5 and in the presence of the substrate and s-hexylglutathione. cal ... | 2006 | 16630751 |
| transcriptomics tool for the human schistosoma blood flukes using microarray gene expression profiling. | we report the design, fabrication, and validation of a microarray covering the majority of the schistosoma japonicum and schistosoma mansoni transcriptomes. the oligonucleotide microarray contains 12,166 s. mansoni and 7055 s. japonicum target sequences. a confidence threshold of <or=0.001 (p value) was used in all analyses. the cross hybridization potential of the microarray was representative of 10,362 probes detected in both channels, while 12,052 probes hybridised to s. japonicum and 11,254 ... | 2006 | 16631746 |
| cloning the genes and dna binding properties of high mobility group b1 (hmgb1) proteins from the human blood flukes schistosoma mansoni and schistosoma japonicum. | the parasitic helminth schistosoma mansoni contains three hmgb proteins, hmgb1, hmgb2 and hmgb3, of primary amino acid sequences highly similar to vertebrate proteins. in this report we describe the characterization of the hmgb1 proteins and their genes from s. mansoni and schistosoma japonicum. the deduced amino acid sequences of hmgb1 proteins from both schistosome species are identical, and comprise 176 residues. the proteins contain the two evolutionarily highly conserved hmg-box domains, a ... | 2006 | 16644144 |
| oligonucleotide microarray analysis of strain- and gender-associated gene expression in the human blood fluke, schistosoma japonicum. | schistosomiasis is a chronic and debilitating disease caused by blood flukes (digenetic trematodes) of the genus schistosoma. schistosomiasis japonica, a zoonosis caused by schistosoma japonicum, is endemic to the philippines and china. we utilised a 22,575 feature custom oligonucleotide dna microarray designed from public domain databases of schistosome-expressed sequence tags to explore differential gene expression between the philippine (sjp) and chinese (sjc) strains of s. japonicum, and bet ... | 2006 | 16647836 |
| schistosoma japonicum eggs survive passage through dogs and chickens. | the present study sought to elucidate whether schistosoma japonicum eggs could survive a passage through dogs and chickens. three dogs and three chickens were used for the experiment. the dogs were allowed to eat goat faeces containing 80 s. japonicum eggs per gram. the chickens were given 2 ml of water containing more than 100 s. japonicum eggs per ml. the hatchability of the eggs was 51.8% prior to administration. all faeces were collected for 3 days from the dogs and for 2 days from the chick ... | 2006 | 16650599 |
| dynamics of cd4+cd25+ t cells in spleens and mesenteric lymph nodes of mice infected with schistosoma japonicum. | cd4+cd25+ t cells play a major role in modulating immune response, but few reports have been published about schistosomiasis. here, we investigated the changes in cd4+cd25+ t cell populations in spleens and mesenteric lymph nodes of mice infected with schistosoma japonicum. the proportions of cd4+cd25+ t cells in total cd4+ t cells were analyzed by flow cytometry. cd25 and foxp3 expression was measured by real-time quantitative polymerase chain reaction. the suppressive activities of cd4+cd25+ t ... | 2006 | 16680369 |