Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| on the chemistry of beta-lactamase inhibition by 6 beta-bromopenicillanic acid. | 1980 | 6253512 | |
| behavior of pathogenic bacteria in the oyster, crassostrea commercialis, during depuration, re-laying, and storage. | oysters (crassostrea commercials) harvested from major cultivation areas within the state of new south wales, australia, were commonly contaminated with low levels of the food-poisoning organisms bacillus cereus, clostridium perfringens, and vibrio parahaemolyticus. salmonella was found in oysters on only one occasion. these bacteria were cleansed from oysters during oyster purification by re-laying in a non-polluted waterway. oysters were laboratory contaminated to levels in excess 1,000 cells ... | 1980 | 6257164 |
| [food bacteriology in madagascar. laboratory activities from 1965 to 1977. techniques and results]. | 1980 | 6263201 | |
| [microbial flora of beef extract imported from australia, uruguay, italy and brazil (author's transl)]. | 1980 | 6265014 | |
| a possible role of stimulus-enhanced phosphatidylinositol turnover: calcium-sparing effect of diacylglycerol in inducing phase separation of phosphatidyl-choline/phosphatidylserine mixtures. | 1981 | 6266405 | |
| immunological detection of food-poisoning toxins. | 1980 | 6269224 | |
| food borne disease. | 1981 | 6271107 | |
| studies on (k+ + h+)-atpase. iv. effects of phospholipase c treatment. | (1) the total phospholipid content of a gradient purified (k+ + h+)-atpase preparation from pig gastric mucosa is 105 mumol per 100 mg protein, and consists of 29% sphingomyelin, 29% phosphatidylcholine, 28% phosphatidylethanolamine, 10% phosphatidylserine and 4% phosphatidylinositol. the cholesterol content corresponds to 50 mumol per 100 mg protein. (2) treatment with phospholipase c (from clostridium welchii and bacillus cereus) results in an immediate decrease of the phosphate content. up to ... | 1981 | 6272855 |
| interaction of beta-lactamase of streptomyces cacaoi. ii. cp-45,899, izumenolide and cephamycins. | inhibition of a beta-lactamase of streptomyces cacaoi by cp-45,899, izumenolide and cephamycins was investigated and compared with that of a beta-lactamase of bacillus cereus. s. cacaoi enzyme could not hydrolyze cp-45,899. instead, hydrolysis of benzylpenicillin by the enzyme was inhibited in the presence of cp-45,899. although inhibition increased gradually with time, the inhibition line produced by cp-45,899 with time less curved than that produced by clavulanic acid and ps-5. furthermore, pr ... | 1981 | 6273376 |
| unusual copper-induced sensitization of the biological damage due to superoxide radicals. | 1981 | 6273398 | |
| enzymatic hydrolysis of immobilized sphingomyelin by three bacterial phospholipases c. | through hydrophobic interaction, sphingomyelin was adsorbed to agarose beads containing octyl groups by a stepwise dilution procedure. this immobilized lipid was used as a substrate for three bacterial phospholipases c (e.c. 3.1.4.3.). the degradation with time of this substrate showed two different fractions of the substrate according to hydrolysing velocity in the early part of the time-curve when phospholipases c from bacillus cereus and clostridium perfringens were used. the early fractions ... | 1981 | 6274145 |
| enzymatic hydrolysis by bacterial phospolipases c and d of immobilized radioactive sphingomyelin and phosphatidylcholine. | an assay system for phospholipases c has been described with sphingomyelin immobilized to octyl-sepharose cl-4b as substrate. the immobilization procedure was further developed and used with [14 c-choline]-sphingomyelin and [14c-choline] phosphatidylcholine (lecithin). these immobilized radioactive phospholipids made the enzymatic assays easier to perform and made it possible to increase the sensitivity. furthermore, since release of the choline part instead of the phosphate part of the substrat ... | 1981 | 6274146 |
| induction and repression of enzymes involved in exogenous purine compound utilization of bacillus cereus. | 5'-nucleotidase, adenosine phosphorylase, adenosine deaminase and purine nucleoside phosphorylase, four enzymes involved in the utilization of exogenous compounds in bacillus cereus, were measured in extracts of this organism grown in different conditions. it was found that adenosine deaminase is inducible by addition of adenine derivatives to the growth medium, and purine, nucleoside phosphorylase by metabolizable purine and pyrimidine ribonucleosides. adenosine deaminase is repressed by inosin ... | 1981 | 6274419 |
| in vitro antimicrobial activity evaluation of cefodizime (hr221), a new semisynthetic cephalosporin. | cefodizime (hr221) is a new alpha-methoxyimino cephalosporin developed by hoechst-roussel with a reported serum half-life of over 2 h. in vitro susceptibility studies showed that the cefodizime spectrum includes all those enterobacteriaceae, staphylococci, streptococcus spp., haemophilus spp., and neisseria spp. normally susceptible to cefotaxime (hr756) or ceftizoxime (fk749) or both. cefodizime was less active (two- to eightfold) than cefotaxime or ceftizoxime against some enteric species, but ... | 1981 | 6275785 |
| [a preliminary report on the indicator of the enterovirus in hospital sewage (comparative studies on the resistance of f2 coliphage, b. cereus spore and hbsag to chemical agents) (author's transl)]. | 1981 | 6277578 | |
| molecular properties and kinetic studies on sphingomyelinase of bacillus cereus. | a sphingomyelinase of bacillus cereus was purified to a homogeneous state (512 u/mg, 2200-fold) as indicated by sds-polyacrylamide gel electrophoresis and the molecular weight (23,300) was determined by sedimentation equilibrium. the enzyme contained loosely-bound magnesium atom. the addition of mg2+ accelerated the enzyme reaction regardless of substrates and their physical state. the addition of ca2+ also accelerated the enzyme reaction slightly, when water-soluble substrates, i.e., 2-hexadeca ... | 1982 | 6284239 |
| wet and dry bacterial spore densities determined by buoyant sedimentation. | the wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of high chemical density. with metrizamide or renografin, the wet density values for the spores and permeable sephadex beads were higher than those obtained by a reference direct mass method, and some spore populations were separated into several density bands. with percoll, all of the wet density value ... | 1982 | 6285824 |
| gram-positive bacteria-induced granulocytopenia and pulmonary leukostasis in rabbits. | pneumococci have been shown to induce granulocytopenia and pulmonary leukostasis which might contribute to morbidity and mortality in pneumococcal sepsis. we studied whether other gram-positive species (groups a and b streptococci, staphylococcus aureus, bacillus cereus, and clostridium perfringens) could also induce these phenomena. rabbits were challenged with nonviable preparations of each species, and all five species induced profound granulocytopenia (mean decrease of 90%) and pulmonary leu ... | 1982 | 6286493 |
| cholinephosphotransferase activities in microsomes and neuronal nuclei isolated from immature rabbit cerebral cortex: the use of endogenously generated diacylglycerols as substrate. | a neuronal nuclear fraction (n1) and a microsomal fraction (p3) were isolated from homogenates of cerebral cortices of 15-day-old rabbits. a nuclear envelope fraction (e) was prepared from n1. to assay cholinephosphotransferase, diacylglycerols were first generated in the membranes of these subfractions using a phospholipase c (bacillus cereus) preincubation. with levels of endogenous diacylglycerols producing maximal specific cholinephosphotransferase activities, an activity ratio of 1:1:5 was ... | 1982 | 6288206 |
| 6-beta-(trifluoromethane sulfonyl)-amido-penicillanic acid sulfone: a potent inhibitor for beta-lactamases. | 1982 | 6288463 | |
| phospholipase c from bacillus cereus has sphingomyelinase activity. | purified, electrophoretically homogeneous phospholipase c (plc) preparations can be separated into two peaks by isoelectric focusing in sucrose gradients. the main peak has an isoelectric ph of 6.6-6.8 and contains two zn2+ per molecule. the more acid peak (isoelectric ph about 6.2) contains about one zn2+ per molecule and has a markedly reduced specific activity which can be raised by adding zn2+. the purified enzyme has a low sphingomyelinase activity which coincides completely with the lecith ... | 1982 | 6291130 |
| the inactivation of bacillus cereus 569/h bera-lactamase by 6-beta-(trifluoromethane-sulfonyl)amidopenicillanic acid sulfone: ph dependence and stoichiometry. | 1982 | 6294040 | |
| attenuation of enkephalin activity in neuroblastoma x glioma ng108-15 hybrid cells by phospholipases. | the role of membrane phospholipids in enkephalin receptor-mediated inhibition of adenylate cyclase (ec 4.6.1.1) activity in neuroblastoma x glioma ng108-15 hybrids was studied by selective hydrolysis of lipids with phospholipases. when ng108-15 cells were treated with phospholipase c from clostridium welchii at 37 degrees c, an enzyme concentration--dependent decrease in adenylate cyclase activity was observed. the basal and prostaglandin e1 (pge1)-stimulated adenylate cyclase activities were mo ... | 1983 | 6294248 |
| transfer of bacillus thuringiensis plasmids coding for delta-endotoxin among strains of b. thuringiensis and b. cereus. | the recently discovered high-frequency transfer of plasmids between strains of bacillus thuringiensis was used to study the genetic relationship between plasmids and production of the insecticidal delta-endotoxin crystal. three strains of b. thuringiensis transmitted the cry+ (crystal-producing) phenotype to cry- (acrystalliferous) b. thuringiensis recipients. agarose gel electrophoresis showed that one specific plasmid from each donor strain was always present in cry+ "transcipients." the size ... | 1982 | 6294667 |
| the role of phospholipids in the binding of oxytocin to its receptors in lactating rabbit mammary gland. | phospholipase c (clostridium welchii and bacillus cereus) treatment of lactating rabbit mammary gland membranes (140,000 g pellet and sucrose density gradient purified plasma membranes) resulted in a large decrease in the binding of [3h]oxytocin to these subcellular fractions. this decrease was not due to a solubilization of oxytocin receptors but was the result of the removal of phospholipids which may participate in the hormone-receptor interaction. phospholipase c treatment of the membrane fr ... | 1983 | 6298074 |
| interaction of beta-iodopenicillanate with the beta-lactamases of streptomyces albus g and actinomadura r39. | the beta-lactamases of streptomyces albus g and actinomadura r39 are inactivated by beta-iodopenicillanate. however, in contrast with the beta-lactamase i from bacillus cereus, they also efficiently catalyse the hydrolysis of the inactivator; with the s. albus g enzyme, kcat. is larger than 25s-1 and the number of turnovers before inactivation is 515. with the a. r39 enzyme, kcat. is larger than 50s-1 and the number of turnovers before inactivation is 80. after hydrolysis of the beta-lactam amid ... | 1982 | 6299270 |
| induction of phosphoribomutase in bacillus cereus growing on nucleosides. | in this paper we show that phosphoribomutase is induced in bacillus cereus by the same metabolizable purine and pyrimidine ribonucleosides previously shown to induce the purine nucleoside phosphorylase (tozzi, m.g., sgarrella, f. and ipata, p.l. (1981) biochim. biophys. acta 678, 460-466). the mutase allows ribose 1-phosphate formed from nucleosides to be utilized by the cell through the pentose cycle, upon transformation to ribose 5-phosphate. the equilibrium constant of the mutase reaction is ... | 1983 | 6299371 |
| induction of ornithine decarboxylase by treatment of guinea pig lymphocytes with phospholipase c. | treatment of guinea pig lymphocytes with clostridium perfringens phospholipase c but not with naja naja snake venom phospholipase a2 increased ornithine decarboxylase activity. the increase in ornithine decarboxylase activity was suppressed by actinomycin d or cycloheximide, suggesting that de novo syntheses of rna and protein are necessary for the increase in the enzyme activity. these results suggest that the activation of phospholipase c rather than that of phospholipase a2 is responsible for ... | 1983 | 6299785 |
| determination of cefmenoxime in human body fluids by high-performance liquid chromatography. | a rapid and reliable method for the quantitative determination of cefmenoxime in serum and urine by reversed phase high-performance liquid chromatography is described. serum was deproteinized with acetonitrile. urine was diluted with dilute acetic acid (17.5 mmol/l). separations were performed in isocratic mode using a c18 type column and a precolumn packed with perisorb rp/8. the eluant consisted of a mixture of acetonitrile and 25.0 mmol/l acetic acid in a ratio of 32/69 (vol/vol). in normal s ... | 1983 | 6301809 |
| increase in osmotic fragility of bovine erythrocytes induced by bacterial phospholipases c. | bovine erythrocytes were treated with each of three bacterial phospholipases c; phosphatidylcholine-hydrolyzing phospholipase c (pcase) of clostridium perfringens, sphingomyelinase c (smase) of bacillus cereus and phosphatidylinositol-specific phospholipase c (piase) of bacillus thuringiensis. an increase in osmotic fragility was detected by means of a coil planet centrifugation (cpc) apparatus (biomedical systems co., tokyo) after the treatment with these enzymes. the peak of hemolysis normally ... | 1983 | 6302097 |
| adsorption of sphingomyelinase of bacillus cereus onto erythrocyte membranes. | sphingomyelinase of bacillus cereus proved to be specifically adsorbed onto mammalian erythrocyte membranes in the presence of either ca2+ or ca2+ plus mg2+ in the order of sphingomyelin content; i.e., sheep, bovine greater than porcine greater than rat erythrocytes. no appreciable adsorption was observed in the presence of mg2+ alone nor in the absence of divalent metal ions. the enzyme adsorption onto bovine erythrocytes was dependent upon the incubation temperature. by shifting the temperatur ... | 1983 | 6305273 |
| bacillus cereus 569/h penicillinase serine-44 acylation by diazotized 6-aminopenicillanic acid. | penicillinase from bacillus cereus 569/h was purified to homogeneity. its active site was probed by use of an affinity label generated in situ by the diazotization of 6-aminopenicillanic acid, a catalytically poor substrate for this enzyme. the loss of activity arising during the inactivation is dependent upon ph and the penicillin:sodium nitrite ratio used. optimal inactivation was obtained at ph 4.7 and reactivation could be prevented if subsequent purification and manipulations were performed ... | 1983 | 6305423 |
| streptococcus plasmid pam alpha 1 is a composite of two separable replicons, one of which is closely related to bacillus plasmid pbc16. | a tetracycline resistance plasmid of streptococcus faecalis, pam alpha 1, is shown to contain two independent sets of replication functions, separated from each other on either side by short (300- to 400-base-pair) sequences of homology. the homologous sequences are oriented as direct repeats and therefore permit the dissociation of pam alpha 1 into its component replicons, referred to here as pam alpha 1 delta 1 and pam alpha 1 delta 2, as the reciprocal products of a simple intramolecular reco ... | 1983 | 6307973 |
| cloning and expression in escherichia coli and bacillus subtilis of the hemolysin (cereolysin) determinant from bacillus cereus. | from a cosmid gene bank of bacillus cereus gp4 in escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. these clones contained recombinant cosmids with b. cereus dna insertions of varying lengths which shared some common restriction fragments. the smallest insertion was recloned as a psti fragment into pjkk3-1, a shuttle vector which replicates in bacillus subtilis and e. coli. when this recombinant plasmid (pjkk3-1 hly-1) ... | 1983 | 6307975 |
| molecular cloning and nucleotide sequence of the type i beta-lactamase gene from bacillus cereus. | the gene for the type i beta-lactamase from bacillus cereus has been cloned in bacillus subtilis and escherichia coli. in b. subtilis, penicillinase activity is detected and the enzyme is secreted. in e. coli, the gene confers ampicillin resistance. the cloned insert is 4.3 kb in length and dna sequencing has revealed the location of the gene, its promoter and signal peptide. | 1983 | 6308567 |
| the action of sphingomyelinase of bacillus cereus on bovine erythrocyte membrane and liposomes. specific adsorption onto these membranes. | sphingomyelinase of bacillus cereus was specifically adsorbed onto sphingomyelin liposome in the presence of ca2+ or with the coexistence of ca2+ and mg2+, but not onto the liposome of phosphatidylcholine. in the presence of ca2+, the enzyme adsorption onto bovine erythrocytes and liposome increased with an increase in the amount of sphingomyelin. these results support that the major binding site for sphingomyelinase in the erythrocytes is sphingomyelin. the temperature-dependence of enzyme adso ... | 1983 | 6309753 |
| [biological effects of coordination compounds of transitional metals. the beta-lactamase inhibitory effect of cis-platin, 2-aminopyridine palladium chloride, clavulanic acid and penicillanic acid sulfonate cp 45,899 in the nitrocefin test and titertek/microtiter automatic system]. | the beta-lactamase-inhibiting activity of 6m-ethyl-pyrid-2-yl-ammine palladium-dichloride (pd 25681) and cis-dichloro-diammine-platinum(ii) was studied and compared with the enzyme inhibitory action of potassium clavulanate and the penicillanic acid sulfone cp 45899. using the nitrocefin test method and the titertek/microtiter equipment cp 45899 and potassium clavulanate were the strongest inhibitors of the bacillus cereus beta-lactamase. cis-dichloro-diammine-platinum(ii) was fourfold less acti ... | 1983 | 6312702 |
| inactivation of bacillus cereus 569/h beta-lactamase i by 6-beta-(trifluoromethane sulfonyl)amidopenicillanic acid sulfone and its n-methyl derivative. | 6-beta-(trifluoromethane sulfonyl)amidopenicillanic acid sulfone and its n-methyl derivative were found to be potent inhibitors of bacillus cereus 569/h beta-lactamase i. the rate of the inactivation of the enzyme by both compounds was found to increase with the decreasing ph of the reaction medium. the reaction of the enzyme with 6-beta-(trifluoromethane sulfonyl)amidopenicillanic acid sulfone was found to be irreversible at the ph values investigated. in contrast, the reaction with the n-methy ... | 1983 | 6315063 |
| survival of cultured cells after functional and structural disorganization of plasma membrane by bacterial haemolysins and phospholipases. | lesions were induced in the plasma membranes of cultured human fibroblasts by membrane damaging toxins of bacterial origin (haemolysins). structural disorganization of the membrane was measured as leakage of a radiolabelled small cytoplasmic marker and functional membrane damage was measured as decreased uptake of aminoisobutyrate. cell survival was scored 24 and 48 hr later by measuring uptake of trypan blue and by light microscopical evaluation of cell morphology and proliferation. the membran ... | 1983 | 6318394 |
| the microbiology of selected retail food products with an evaluation of viable counting methods. | in an inter-laboratory survey, the pour plate, surface spread, agar droplet and spiral plate methods were used in parallel with the surface drop method for enumeration of micro-organisms in foods. good agreement was obtained between all surface methods of enumeration, but there was poor agreement between molten agar methods and the surface drop method. a total of 1143 samples of food that were ready for consumption at the point of retail sale were examined. eight types of food products were chos ... | 1984 | 6319486 |
| [relations between food poisoning and ascariasis]. | during the examination of faeces for the causative agents of food poisoning, the search was also extended to the occurrence of worm eggs. ascaris lumbricoides eggs occurred six times more frequently in persons affected with food poisoning than in consumers who were not harmed by the intake of poisoned food. this relationship was observed in non-specific cases of food poisoning and in those of unknown aetiology, whereas it was less pronounced in cases of specific poisoning. this difference has no ... | 1983 | 6322473 |
| enzyme immunoassays in which biotinillated beta-lactamase is used for the detection of microbial antigens. | the performance characteristics of enzyme immunoassays are determined to a great extent by the enzyme-substrate system utilized for the immunoassay. beta-lactamases (penicillin amido-beta-lactamhydrolase ec 3.5.2.6) offer a number of advantages which might make them useful in immunoassay systems. we linked beta-lactamase from bacillus cereus with biotin and used the biotinillated enzyme to devise immunoassay systems for the detection of a number of microbial antigens. an assay system in which an ... | 1984 | 6325489 |
| comparative study of the nature and biological activities of bacterial enterotoxins. | it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ... | 1984 | 6327986 |
| physiological responses of bacillus species to concanavalin a. 2. effect on growth, oxygen uptake, enzyme activities and intracellular cyclic guanosine 3',5'-monophosphate level of b. cereus atcc 14579. | the effect of the mitogen concanavalin a (con a) on growth and several physiological aspects of bacillus cereus atcc 14579 was investigated. con a at concentrations ranging from 50 to 750 micrograms/ml stimulated growth (the growth rate increased from 0.52/h to 0.97/h, and final yield increased by 2.3-fold over the control) of the bacterial cells. con a-treated cells also increased their oxygen uptake (1.6-fold increase when treated with 750 micrograms/ml of con a). the activities of the membran ... | 1984 | 6328227 |
| [history of clostridium perfringens food poisoning]. | 1984 | 6328647 | |
| kinetics of inactivation of beta-lactamase i by 6 beta-bromopenicillanic acid. | the kinetics of the inactivation of beta-lactamase i from bacillus cereus 569 by preparations of 6 alpha-bromopenicillanic acid showed unexpected features. these can be quantitatively accounted for on the basis of the inactivator being the epimer, 6 beta-bromopenicillanic acid. at ph 9.2, the rate-determining step in the inactivation is the formation of the inactivator. when pure 6 beta-bromopenicillanic acid is used to inactivate beta-lactamase i, simple second-order kinetics are observed. the ... | 1980 | 6331385 |
| purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose. | several beta-lactamases, enzymes that play an important part in antibiotic resistance, have been purified by affinity chromatography on boronic acid gels. the procedure is rapid, appears to be selective for beta-lactamases, and allows a one-step purification of large amounts of enzyme from crude cell extracts. we have found the method useful for any beta-lactamase that is inhibited by boronic acids. two kinds of boronic acid column have been prepared, the more hydrophobic one being reserved for ... | 1984 | 6332621 |
| [microbiological study of spices. i. oregano]. | a total of 150 oregano samples from 6 different trade marks were studied. these samples were obtained at retail shops in buenos aires city and they were analyzed in order to determine the presence of the following microorganisms: 1) mesophilic aerobic plate count; 2) molds; 3) coliforms and fecal coliforms; 4) enterococci; 5) aerobes sporeforming: a) mesophilic aerobes; a1) total aerobes; a2) mesophilic anaerobes; b) thermophilic; b1) sulfide spoilage sporeformers; b2) total aerobes; b3) thermo- ... | 1984 | 6336363 |
| evaluation of the direct epifluorescent filter technique for assessing the hygienic condition of milking equipment. | the hygienic condition of 6 milking installations, 3 sanitized by circulation cleaning (cc) with chlorine-based chemicals and 3 by flushing with acidified boiling water (abw), was tested using rinses of quarter strength ringer's solution. the bacterial content of the rinses was determined using both colony counts and the direct epifluorescent filter technique (deft). a comparison of testing methods gave correlation coefficients between colony count and deft of 0.82 for plants using cc and 0.46 f ... | 1983 | 6341424 |
| effects of nitric oxide and nitrogen dioxide on bacterial growth. | the effects of low concentrations of nitric oxide (no) and nitrogen dioxide (no2) on actively dividing cultures of staphylococcus aureus, micrococcus luteus, micrococcus roseus, serratia marcescens, bacillus subtilis, bacillus circulans, bacillus megaterium, and bacillus cereus were studied. fresh cultures of each organism were incubated for 24 h at 25 degrees c on both nutrient agar and mineral salts glucose agar plates under atmospheres containing various low concentrations of no in air (0 to ... | 1983 | 6351744 |
| effect of inhibitors of trypsin-like proteolytic enzymes bacillus cereus t spore germination. | the germination of bacillus cereus t spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective inhibitors, whereas chymostatin, elastatinal, and pepstatin are inactive. a synthetic substrate of trypsin, tosyl-arginine-methyl ester, also inhibits germination. its inhibitory effect decreases as a function of incubation time in the presence of spores and is abolished by previous hydrolysis with try ... | 1983 | 6401704 |
| differentiation between spores of bacillus anthracis and bacillus cereus by a quantitative immunofluorescence technique. | a quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of bacillus anthracis and bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with b. anthracis vollum spores. the spores of 11 of the 20 b. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several b. anthracis isolates tested. however, absorption of the conjugat ... | 1983 | 6402519 |
| necrotizing pneumonia and empyema caused by bacillus cereus and clostridium bifermentans. | a patient with no known immune compromise presented with necrotizing pneumonia and a pleural effusion. thoracentesis yielded a sanguinopurulent, foul smelling exudate that showed sheets of polymorphonuclear leukocytes and many gram-positive bacilli. a tube thoracostomy was performed and treatment with intravenous penicillin g was begun. twenty-four hours later a bacillus species later identified as bacillus cereus was identified from aerobic cultures. because the organism was resistant to penici ... | 1983 | 6402966 |
| [toxigenicity of microorganisms isolated from dry products for infant and dietetic nutrition]. | the authors describe the results of studying the dissemination with bac. cereus cells and staphylococci of raw material, components and ready-made products for infants' and dietetic nutrition after varying times of storage under the conditions of uncontrolled humidity and temperature. it has been demonstrated that bac. cereus and staphylococcal strains isolated are potentially enterotoxigenic. prevention of food poisoning with the microorganisms in question should be aimed at the improvement of ... | 1983 | 6404059 |
| catalytic properties of sepharose-bound l-alanine dehydrogenase from bacillus cereus. | (1) l-alanine dehydrogenase from bacillus cereus was purified by a two-step chromatographic procedure involving cibacron-blue 3g-a sepharose 4b-cl, and sepharose 6b-cl, and immobilized on cnbr-activated sepharose 4b. (2) following immobilization via two of the six subunits, l-alanine dehydrogenase retained 66% of the specific activity of the soluble enzyme. the affinity of the immobilized enzyme for nh4+, pyruvate and l-alanine, was not different to that of the soluble form. the km of the sephar ... | 1983 | 6404304 |
| structure of teichoic-acid--glycopeptide complexes from cell walls of bacillus cereus ahu 1030. | from lysozyme digests of n-acetylated cell walls of bacillus cereus ahu 1030, two acidic polymer fractions with molecular weights of about 24000 and 45000 were isolated by ion-exchange chromatography and gel chromatography. these polymer fractions, containing glycerol, phosphorus and glucose in a molar ratio of 1.00:1.00:0.85 together with small amounts of glycopeptide components and mannosamine, were characterized as teichoic-acid-glycopeptide complexes with one and two teichoic acid chains mad ... | 1983 | 6404629 |
| [characteristics of the autoregulatory factor d2 causing autolysis of pseudomonas carboxydoflava and bacillus cereus cells]. | in the course of organoheterotrophous growth, pseudomonas carboxydoflava z-1107 and bacillus cereus 504 were found to synthesize the autoregulatory factor d2, a membranotropic lipid metabolite, and to accumulate it in the growth medium. at a low concentration, the factor activated respiration of the cells: at a high concentration, it inhibited respiration and induced autolysis of the cells. the physiological action of d2 was shown to be due to the effect of free fatty acids, in particular, palmi ... | 1983 | 6405132 |
| recent advances in management of bacterial diarrhea. | the number of recognized infectious causes of diarrhea potentially treatable with specific antibiotics has markedly increased within the past ten years. laboratories are developing and expanding their abilities to deal with these new pathogens. neither prophylaxis nor specific treatment of diarrhea in travelers is simple, practical, and safe. although enterotoxigenic escherichia coli is the most important cause of diarrhea in u.s. travelers to tropical areas, campylobacter jejuni causes acute di ... | 1983 | 6405474 |
| [in vitro study of the lipase activity of bacterial strains isolated from the rabbit cecum on c14-labeled triolein]. | the lipase activity of bacterial species isolated from the caecum of conventional rabbits was studied by means of two groups of tests: (1) bacteriologic isolation and an identification of nine aerobic and anaerobic clones and (2) incubation of each of these clones in the appropriate medium in the presence of labelled triolein (oleyl 1-14c), a biochemical analysis of the radioactivity of the fractions obtained. the main results can be expressed in the following way: maximum hydrolysis of glycerid ... | 1983 | 6405674 |
| lactoferrin and transferrin fragments react with nitrite to form an inhibitor of bacillus cereus spore outgrowth. | tryptone is a pancreatic digest of casein which contains a heterogeneous mixture of substances that react with nitrite when heated in the presence of sodium thioglycolate to form a bacteriostatic agent which inhibits outgrowth of bacillus cereus t spores. the substances which are precursors to the bacteriostatic agent can be fractionated on the basis of molecular size and charge and have properties which indicate that they are fragments of lactoferrin, an iron-binding glycoprotein. the bacterios ... | 1983 | 6405692 |
| ampicillin induced septum formation in bacillus cereus. | cells of bacillus cereus grown in the presence of subinhibitory concentrations of ampicillin at either 30 degrees or 45 degrees c exhibited an increase in the numbers of centres of septum formation per unit cell length. under identical conditions of cultivation, cells of escherichia coli grew as aseptate filaments. in general, untreated b. cereus cells grown at 45 degrees c were longer than those grown at 30 degrees c. the strain of e. coli used was unaffected in terms of filamentation by elevat ... | 1983 | 6406798 |
| meningitis and bacteremia due to bacillus cereus. a case report and a review of bacillus infections. | a patient with meningitis and bacteremia due to bacillus cereus is described. the patient had transsphenoidal hypophysectomy for chromophobe adenoma, complicated by rhinorrhea, which was corrected by subarachnoid drainage. three weeks after removal of the drain, the patient presented with meningitis and died the following day. the causative organism was identified as b. cereus. the literature on bacillus infections is reviewed with special attention to severe infections. a modified classificatio ... | 1983 | 6408023 |
| bacterial enteritis. | 1983 | 6408371 | |
| determination of dipicolinic acid in bacterial spores by derivative spectroscopy. | dipicolinic acid was extracted from approximately 0.1 mg spores or 0.5 ml of sporulating culture with 20 mm hcl for 10 min at 100 degrees c. the suspension was diluted with 5 mm ca2+, 100 mm tris, ph 7.6, centrifuged, and the first derivative of the uv absorbance spectrum recorded from 275 nm to 285 nm. dpa concentration was determined from the difference between the maximum at 276.6 nm and the minimum at 280 nm. the use of the difference between two first derivative values removed possible inte ... | 1983 | 6408947 |
| bacillus cereus infection in burns. | two patients are reported in whom severe toxicity developed about 4 days after relatively minor burn injuries and in whom the burn areas then appeared to enlarge. in both patients, b. cereus and staph. aureus were isolated and the affected burn areas had subcutaneous thrombosis and necrosis. the management is outlined and the dramatic rapidity of onset of toxicity emphasized, with special reference to increasing pain, lividity and extension of the burns. | 1983 | 6409357 |
| the antimicrobial effect of dissociated and undissociated sorbic acid at different ph levels. | the minimum inhibitory concentration of sorbic acid has been determined for bacillus subtilis, bacillus cereus, escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and candida albicans. the inhibition was shown to be due to both undissociated and dissociated acid, and the effect of each has been calculated in accordance with a proposed mathematical model. although the inhibitory action of undissociated acid was 10-600 times greater than that of dissociated acid, the latter caused mor ... | 1983 | 6409875 |
| necrotizing pneumonia and empyema caused by bacillus cereus and clostridium bifermentans. | 1983 | 6410947 | |
| distribution of heterogeneous and homologous plasmids in bacillus spp. | a total of 75 strains (including 5 reference strains) of bacillus amyloliquefaciens, b. cereus, b. circulans, b. licheniformis, b. megaterium, b. pumilus, b. sphaericus, b. subtilis, and b. thuringiensis and 36 species-unidentified bacillus strains were surveyed for plasmids by cesium chloride-ethidium bromide equilibrium centrifugation of cell lysates in a study of antibiotic resistance in host cells. of the 111 strains, 13 (including 3 reference strains) were found to harbor plasmids, and 5 of ... | 1983 | 6410988 |
| use of chromatofocusing for separation of beta-lactamases. iii. beta-lactamases of the bacillus cereus 569/h strain. | 1983 | 6411752 | |
| penetration of oxygen into bacterial colonies. | previous estimates of the depth of oxygen penetration into bacterial colonies were made after measuring actual and potential respiration rates of whole colonies, or by calculation from kinetic values determined from the growth of bacteria in liquid culture. this paper reports the use of microelectrodes to measure oxygen penetration directly. oxygen became undetectable 25-30 microns below the surface of a 120 microns deep, 18 h colony of bacillus cereus. the colony was grown on a nutrient-rich ag ... | 1983 | 6411858 |
| the bacterial flora of candling-reject and dead-in-shell turkey eggs. | the incidence of bacterial contamination in turkey eggs which were rejected at candling or were "dead in shell" was about 4%. this incidence was lower than that previously found in chicken eggs. analysis of the bacterial flora indicated that the proportion of enterobacteriaceae was higher and the proportion of micrococcus spp. lower in turkey eggs. | 1983 | 6412969 |
| bacillus cereus 569/h beta-lactamase i: cloning in escherichia coli and signal sequence determination. | the gene, penpc, for beta-lactamase i of bacillus cereus 569/h has been cloned and its expression studied in escherichia coli. the protein product from the in vitro translation of penpc was shown by gel electrophoresis to have an mr of 36 000 which is larger than the in vivo products found in b. cereus and e. coli. the dna sequence of the signal region was determined. it revealed that the smallest known mature form present in b. cereus culture fluids is preceded by 45-48 amino acids in pre-beta- ... | 1983 | 6413253 |
| non-gastrointestinal bacillus cereus infections: an analysis of exotoxin production by strains isolated over a two-year period. | isolates of bacillus cereus from 118 cases, and two maternity unit outbreaks, of non-gastrointestinal infection were grouped on the basis of their estimated probable involvement in the infections from which they were isolated: (i) high probability--48 strains; (ii) intermediate--16 strains; (iii) low--7 strains; (iv) very low ("irrelevant")--49 strains. rabbit skin test, haemolysin and phospholipase assays were used to determine exotoxin activities of strains within each group. the results sugge ... | 1983 | 6413550 |
| [membrane-active properties of a preparation from a bacterial culture broth possessing autoregulation action]. | the effect of a preparation obtained by butanol extraction of the culture fluid of b. cereus and ps. carboxydoflava and previously termed an autoregulatory factor d1 on the respiration chain within intact bacterial cells and isolated membranes was investigated. in comparable concentrations this factor d1 inhibits the endogenous respiration of b. cereus and m. lysodeikticus as well as oxidase and dehydrogenase activities of isolated membranes from these bacteria and e. coli. the factor-induced de ... | 1983 | 6413971 |
| characterization of the membrane beta-lactamase in bacillus cereus 569/h/9. | the membrane-bound beta-lactamase from bacillus cereus, strain 569/h/9, has been purified to apparent homogeneity. nonionic detergent (0.5% triton x-100) is required to keep the enzyme (traditionally called gamma-penicillinase and now called beta-lactamase iii) in solution. antibodies to beta-lactamase iii have been prepared, and the membrane-bound enzyme is immunochemically distinct from the extracellular enzymes. beta-lactamase iii has a molecular weight of 31 500, in contrast to the extracell ... | 1983 | 6414514 |
| beta-lactamase iii of bacillus cereus 569: membrane lipoprotein and secreted protein. | a third beta-lactamase in bacillus cereus 569 has been identified and characterized. it corresponds to gamma-penicillinase reported by pollock [pollock, m. r. (1956) j. gen. microbiol. 15, 154-169] but whose existence has been questioned since then. it will be called beta-lactamase iii. it resembles the class a beta-lactamases but is immunologically distinct from the major class a secreted beta-lactamase i of b. cereus. as with several other gram-positive beta-lactamases it occurs in two forms, ... | 1983 | 6414515 |
| immunofluorescence analysis of bacillus spores and vegetative cells by flow cytometry. | a commercially available flow cytometer (cytofluorograf) was used for the immunofluorescence (if) analysis of spores of bacillus anthracis, bacillus cereus, and bacillus subtilis, using fluorescein-labelled antispore conjugates. the cytometer was modified to allow analysis of known numbers of bacteria. in attempting to identify the region of the cytometer fluorescence histogram associated with the presence of stained spores, evidence was produced for signal components due to antibody bound to ex ... | 1983 | 6414791 |
| postincision steps of photoproduct removal in a mutant of bacillus cereus 569 that produces uv-sensitive spores. | an excision-defective mutant of bacillus cereus 569 is normal in incision and repair synthesis, but rejoining of incision breaks is defective, resulting in accumulation of low-molecular-weight dna after uv irradiation. the defect in removal of photoproducts by exonuclease after incision renders both vegetative cells and dormant spores of the mutant sensitive to uv. a similarity is indicated to the uvrd mutation described recently in escherichia coli. | 1983 | 6415041 |
| [is food poisoning caused by bacillus cereus more frequent?]. | 1983 | 6416806 | |
| [bacteria decomposing alpha-methylstyrol]. | two bacterial strains assimilating alpha-methylsterene as a sole carbon and energy source were isolated from the sewage of an industrial plant producing synthetic rubber. the morphological, cultural and physiologo-biochemical properties of the strains were studied and their taxonomic position was determined. one of the cultures was classified as bacillus cereus and the other as pseudomonas aeruginosa. the rate of alpha-methylsterene destruction in the chemically defined medium was shown to depen ... | 1983 | 6417462 |
| food-borne infections and intoxications--recent trends and prospects for the future. | 1983 | 6417799 | |
| studies on the penetration of lymecycline into the paranasal sinus in man. | concentration of lymecycline, a lipophilic tetracycline, in the human sinusal mucosa tissue, as measured by the "punch method" technique are fairly high. the median value of all examined pieces of mucosa amounts to 1.2 mcg/g. | 1983 | 6417972 |
| [transformation of bacillus cereus protoplasts with the plasmid dna from bacillus thuringiensis transformants]. | 1983 | 6417981 | |
| thermal denaturation of native and cross-linked bacillus cereus 569/h beta-lactamase i. | thermal denaturation of native and internally cross-linked bacillus cereus 569/h beta-lactamase i (beta-lactamhydrolase, ec 3.5.2.6) was investigated using differential scanning calorimetry. application of temperature-scanning kinetics provided an estimate of various activation parameters for the denaturation process. evidence is presented to indicate that subtle temperature-induced conformational changes preceding gross denaturation are sufficient to cause inactivation of the enzyme. | 1983 | 6418209 |
| [interspecific recombinants of bacillus thuringiensis x bacillus cereus]. | the possibility of interspecies recombination was shown by using protoplast fusion method. the bacillus thuringiensis var. galleriae strain 48s thi nic gua rifr strr and 56r gua rifr, and also bac. cereus carrying the plasmid pbc16 responsible for resistance to tetracycline (150 mcg/ml) were used. recombinants were selected on the medium containing rifampicin and tetracycline. they were shown to combine the properties of both parents. the majority of recombinants were resistant to phages tg4 and ... | 1983 | 6418614 |
| differentiation of bacillus anthracis and other bacillus species by lectins. | bacillus anthracis was agglutinated by several lectins, including those from griffonia simplicifolia, glycine max, abrus precatorius, and ricinus communis. some strains of bacillus cereus var. mycoides (b. mycoides) were strongly reactive with the lectin from helix pomatia and weakly reactive with the g. max lectin. the differential interactions between bacillus species and lectins afforded a means of distinguishing b. anthracis from other bacilli. b. cereus strains exhibited heterogeneity with ... | 1984 | 6418761 |
| penicillinase secretion in vivo and in vitro. | 1983 | 6419016 | |
| the incidence of bacillus cereus in foods in central thailand. | thirty of 53 different raw and cooked or processed foods obtained in central thailand were demonstrated to contain bacillus cereus. the level of contamination was found to depend on the type of food sampled; the mean was 3.2 x 10(6) organisms/gm on raw and cooked or processed foods ready for consumption, and 2.8 x 10(8) organisms/gm on cooked or processed foods kept overnight at kitchen temperature. b. cereus was commonly found on rice (both uncooked and boiled or fried), dried chilli pepper, sh ... | 1983 | 6419352 |
| [effects of 5-bromouracil and 5-bromodeoxyuridine in combination with 8-aza-adenine on the uv sensitivity of bacteria]. | the presence of 5-bromouracil (bu) as well as 5-bromo-2-deoxyuridine (budr) in the cultivation media of bacteria results in the distinct increase of uv sensitivity. with the nucleic acid base analogue 8-azaadenine (8-aa) a similar effect was confirmed, however, not so pronounced. in the experiments reported here the combined action of bu or budr and 8-aa on escherichia coli, proteus mirabilis, bacillus subtilis and bacillus cereus was investigated. the sensitization effect of budr does not incre ... | 1983 | 6419483 |
| [biochemical characteristics of strains of bacillus cereus]. | 1983 | 6420998 | |
| cooperative changes in the chiroptical properties of dna induced by methanol. | 1984 | 6421338 | |
| changes in membrane-associated proteins during sporulation in bacillus subtilis. | membrane proteins from vegetative and sporulating cells of bacillus subtilis were separated by the two-dimensional gel electrophoresis system using isoelectric focusing and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (o'farrell technique). membrane proteins were isolated according to published procedures. the gels were stained with coomassie blue. three different concentrations of proteins were analyzed to detect even minor constituents. over two hundred different membrane proteins ... | 1984 | 6421583 |
| isolation and some properties of an enterotoxin produced by bacillus cereus. | extracellular proteins produced by bacillus cereus b-4ac were separated by chromatography on amberlite cg-400, qae-sephadex, sephadex g-75, and hydroxylapatite. a fraction, containing three detectable antigens, obtained from chromatography on hydroxylapatite caused fluid accumulation in ligated rabbit ileal loops, was dermonecrotic to rabbit skin, was cytotoxic to cultured cells, and was lethal to mice after intravenous injection. two other fractions obtained from chromatography on hydroxylapati ... | 1984 | 6421739 |
| interspecies transduction of plasmids among bacillus anthracis, b. cereus, and b. thuringiensis. | bacteriophage cp-51, a generalized transducing phage for bacillus anthracis, b. cereus, and b. thuringiensis, mediates transduction of plasmid dna. b. cereus gp7 harbors the 2.8-megadalton multicopy tetracycline resistance plasmid, pbc16. b. thuringiensis 4d11a carries pc194, the 1.8-megadalton multicopy chloramphenicol resistance plasmid. when phage cp-51 was propagated on these strains, it transferred the plasmid-encoded antibiotic resistances to the nonvirulent weybridge (sterne) strain of b. ... | 1984 | 6421798 |
| [effects of photochemical smog from a flow reactor on bacteria. i. determination of the effects of photochemical smog on bacteria]. | to measure the damage to bacteria from photochemical smog serratia marcescens, staphylococcus epidermidis, micrococcus luteus and spores of bacillus cereus have been exposed to defined gas-mixtures. a smog-simulation-chamber has been used which allowed adjustment of reproducible and longterm constant smog formations due to the flow system. two methods have been applied to examine the bactericidal effects of the photo-chemical smog: adsorption of bacteria to membrane filters and spraying on silk ... | 1983 | 6422676 |
| [effects of photochemical smog from a flow reactor on bacteria. ii. determination of bactericidal components in photochemical smog]. | the mixture of substances in the photochemical smog could be detected by different reduction rates of exposed bacteria. beside ozone other products of the ozone/olefine-reaction could reduce the survival of exposed bacteria. for staph. epidermidis a toxic influence from the reaction products could be found only after uv-irradiation. the main components were aldehydes, hydrocarbons, radicals, peroxiradicals and radicaloxides. for peroxiacetylnitrate (pan) no bactericidal effect could be found for ... | 1983 | 6422677 |
| [effect of the addition of dmso and edta on the activity of formaldehyde and hydrogen peroxide against bacillus cereus spores]. | the question was investigated if dmso in concentrations between 1 and 6% and 0.1% edta could improve the effect of the sporocidal disinfectants formaldehyde and hydrogen-peroxide. in suspension-tests with spores of bac. cereus it was found, that the sporocidal activity of a 1.2 and 3% formaldehyde solution did not increase, in contrary it decreased significantly if edta or dmso were present in the solution. the same effect could be found with 0.8-5% hydrogenperoxide in combination with the above ... | 1983 | 6422678 |
| recent developments in separation and purification of biomolecules. | 1983 | 6422824 | |
| a case of septicaemia caused by bacillus cereus. | 1984 | 6423383 | |
| what do beta-lactamases mean for clinical efficacy? | beta-lactamases have proved to be extremely important in influencing therapy with penicillins and cephalosporins against gram-positive and gram-negative aerobic and anaerobic species. both plasmid mediated beta-lactamases which are primarily of a constitutive penicillinase type and the inducible chromosomal enzymes which are primarily cephalosporinases are important. the use of penicillins to treat haemophilus, neisseria gonorrhoeae, escherichia coli, klebsiella, salmonella, shigella and pseudom ... | 1983 | 6423546 |