Publications

activity of e-3846, a new fluoroquinolone, in vitro and in experimental cystitis and pyelonephritis in rats.	the in vitro antibacterial activity of e-3846, a new fluoroquinolone carboxylic acid derivative with a pyrrol ring substituent at position 7, was evaluated in comparison with norfloxacin and ciprofloxacin. e-3846 was more active than the reference quinolones against staphylococcus species, including methicillin-resistant strains. e-3846 was similar to ciprofloxacin and more active than norfloxacin against streptococcus (enterococcus) faecalis. in general, e-3846 was more active than norfloxacin ...	1988	3134844
renal pharmacokinetic changes of gentamicin during enterococcal pyelonephritis.	in the present study, the renal pharmacokinetics of gentamicin was investigated in pyelonephritic rats infected with streptococcus (enterococcus) faecalis. four days after the induction of infection, animals were given either a single dose of gentamicin or two daily injections for 7 days. the treated animals were evaluated from 1 h to 6 months posttreatment. after a single injection, limited pharmacokinetic variations were observed, whereas after 14 injections infected kidneys demonstrated signi ...	1988	3134849
expression of the hemolysin trait in a proteolytic transconjugant of streptococcus faecalis.	streptococcus faecalis 31h-1 is a proteolytic transconjugant of strain 31b, that harbors the 38.5 mdal plasmid which codes for hemolysin in strain x14. it fails to express the hemolytic character in broth due to its proteolytic character. hemolytic activity can be demonstrated when the proteolytic activity is inhibited by 10(-4) m edta. the effect of erythromycin (on the donor cells) and of proteinase k during conjugation suggests that the conjugation system involving the 38.5 mdal plasmid of s. ...	1988	3134952
cefotaxime and flucloxacillin as antibiotic prophylaxis in cardiac transplantation.		1988	3135163
prophylactic use of cefotaxime in biliary surgery. a prospective multicentre comparison of two dosage regimens.		1988	3135165
impact of cefotaxime and ceftriaxone on the bowel and vaginal flora after single-dose prophylaxis in vaginal hysterectomy.	the aim of this randomised controlled study was to investigate the effect of a preoperative, single dose, intravenous injection of ceftriaxone 2g and cefotaxime 2g in 2 groups of 30 patients who underwent vaginal hysterectomy. the parameters were: efficacy and tolerance; impact on the aerobic bowel and vaginal flora; and serum concentrations. there were no significant differences in efficacy, haematology or clinical chemistry parameters. in the ceftriaxone group 2 patients had exanthemas, 2 had ...	1988	3135167
[bactericidal and potent antiadhesive activity of sulbenicillin].	sulbenicillin, a wide broad spectrum penicillin, is active against a lot of gram positive and gram negative bacteria. the aa. studied the activity of this molecule against urinary infections causing germs, by evaluating two parameters: his antiadhesive capability and the killing curves, in comparison with mezlocillin and piperacillin. an inhibition in adhesive capability of test-germs, due to sulbenicillin was obtained. furthermore, resulting killing curves showed more rapidity in action for sul ...	1986	3135222
infective endocarditis amongst intravenous drug abusers seen at the university hospital, kuala lumpur.		1987	3136302
[comparative bactericidal kinetics of ciprofloxacin, ofloxacin and pefloxacin alone and in combination against strains of group d streptococcus].	killing curves method was used to study bactericidal activity of the three quinolones, alone and in combination with penicillin, amoxicillin, fosfomycin, netilmicin, vancomycin against six isolates of enterococci. alone, ofloxacin showed the most initial bactericidal effect but ciprofloxacin was the most frequently bactericidal without any regrowth in 24 hours. the combinations with beta-lactams and netilmicin were sometimes synergistic in 24 hours; fosfomycin increased initial bactericidal effe ...	1988	3136422
[post-antibiotic effect of ciprofloxacin alone and in combination on streptococcus faecalis].	post-antibiotic effect (pae) of ciprofloxacin has been studied on 2 strains of s. faecalis in the logarithmic phase after 2 exposure times and at concentrations ranging 0.5-10 times the mic. at the end of the exposure period the antimicrobial is removed by dilution. similar pae is observed for ciprofloxacin and ofloxacin, higher than for pefloxacin. ciprofloxacin-fosfomycin or ciprofloxacin-vancomycin combinations are indifferent or even antagonistic (vancomycin) in regards to pae. ciprofloxacin ...	1988	3136423
[evolution from 1976 to 1986 of bacterial prevalence and sensitivity to urinary antibiotics of bacteria isolated from urine cultures at a city microbiology laboratory].	the evolution of the prevalence of the bacterial strains isolated from uti cases (bacteriuria greater than or equal to 10(5)/ml has been studied over a 11 year-period from 1976 to 1986, in a clinical practice microbiological laboratory from a rural area. e. coli was the overall most frequently isolated species, followed by p. mirabilis (74.4% and 11.2% respectively), by the klebsiella-enterobacter group (4.8%), and staphylococci (represented by coagulase negative staphylococci) (4.9%). the relat ...	1988	3136425
[phagocytosis of streptococcus faecalis by trichomonas vaginalis. electron microscopy studies].		1987	3136590
in vitro antibacterial activities of eleven antibiotics against s. faecalis.	the in vitro antibacterial activities of penicillin, ampicillin, piperacillin, azlocillin, vancomycin, erythromycin, clindamycin, gentamicin, streptomycin, cefotaxime, and ceftriaxone against 198 s. faecalis strains were investigated, employing an agar dilution technique. results were generally in accord with those of other studies. ampicillin and penicillin showed equal activities. twenty percent of the strains were resistant to erythromycin, which correlated with resistance to clindamycin. the ...	1988	3136781
conjugative plasmid transfer from enterococcus faecalis to escherichia coli.	the possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pbr322-pam beta 1 chimeric plasmid, designated pat191. this shuttle vector, which possesses the tra functions of the streptococcal plasmid pam beta 1, was conjugatively transferred from enterococcus faecalis to escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.	1988	3137216
multiply high-level-aminoglycoside-resistant enterococci isolated from patients in a university hospital.	enterococci isolated from different body sites were tested for high-level gentamicin resistance. a total of 259 enterococcal isolates were screened for resistance (mic, greater than 2,000 micrograms/ml) by a broth-tube method. thirty-nine (15.1%) were found to exhibit resistance and were confirmed by agar screening (1,000 micrograms/ml) and agar dilution mic determinations. the majority of isolates also showed high-level resistance to kanamycin and streptomycin. the remaining isolates showed hig ...	1988	3137246
phosphinic acid inhibitors of d-alanyl-d-alanine ligase.	we report the synthesis of a series of phosphinic acid dipeptide analogues, nh2ch(r1)po(oh)ch2ch(r2)co2h, related to dala-dala. the best of these compounds are potent, essentially irreversible inhibitors of dala-dala ligase, and their preferred stereochemistry was shown by chiral synthesis of (1(s)-aminoethyl)(2(r)-carboxy-1-n-propyl)phosphinic acid, 12b, and by x-ray crystallography of its derivative benzyl [1(s)-[(benzyloxycarbonyl)-amino]ethyl](2(r)-carbomethoxy-1-propyl) phosphinate, 13, to ...	1988	3137344
[the degree of dna homology in enterococci].	studies carried out by the method of dna-dna hybridization have confirmed that enterococcus faecalis, s. faecium and s. durans are independent species. as revealed in these studies, s. faecalis are homogeneous, genetically related and form an independent, sharply defined species, while s. faecium form a heterogeneous species. mobile enterococci with yellow pigmentation must not be classified with both s. faecalis and s. faecium.	1988	3137749
comparative killing activity and postantibiotic effect of streptomycin combined with ampicillin, ciprofloxacin, imipenem, piperacillin or vancomycin against strains of streptococcus faecalis and streptococcus faecium.	nine strains of streptococcus faecalis and streptococcus faecium were studied with respect to ampicillin, ciprofloxacin, imipenem, piperacillin, vancomycin and streptomycin. two strains were highly resistant (mic greater than or equal to 2,000 micrograms/ml) to streptomycin. evaluation and comparison of the killing activity with killing curves, and duration of the postantibiotic effect (pae) after exposure for 1 h with regrowth curves was done with combinations of antibiotics or alone. the overa ...	1988	3138078
aortic root abscess--echocardiographic image.	autopsy findings point to a high prevalence of perivalvular abscesses in patients with infective endocarditis. diagnosis of this complication, which may have therapeutical implications, is difficult. the authors present echocardiographic image obtained in a patient with bacterial endocarditis in whom an aortic root abscess was subsequently found at operation.	1988	3139365
transport of diamines by enterococcus faecalis is mediated by an agmatine-putrescine antiporter.	enterococcus faecalis atcc 11700 is able to use arginine and the diamine agmatine as a sole energy source. via the highly homologous deiminase pathways, arginine and agmatine are converted into co2, nh3, and the end products ornithine and putrescine, respectively. in the arginine deiminase pathway, uptake of arginine and excretion of ornithine are mediated by an arginine-ornithine antiport system. the translocation of agmatine was studied in whole cells grown in the presence of arginine, agmatin ...	1988	3139630
structure of ccf10, a peptide sex pheromone which induces conjugative transfer of the streptococcus faecalis tetracycline resistance plasmid, pcf10.	the peptide pheromone, ccf10, which induces aggregation and high frequency plasmid transfer in streptococcus faecalis cells carrying the tetracycline resistance plasmid, pcf10, was isolated and its structure determined. the molecular weight of ccf10 is 789, and its amino acid sequence is h-leu-val-thr-leu-val-phe-val-oh. pheromone activity, as determined by a clumping induction assay, was detectable at a concentration of 2.5 x 10(-11) m. a peptide of the same sequence as that of the ccf10 produc ...	1988	3139658
antimicrobial and anticancer activity of tetrahedral, chelated, diphosphine silver(i) complexes: comparison with copper and gold.	tetrahedral, bischelated ag(i) diphosphine complexes [ag(p-p)2]no3, where p-p is ph2p(ch2)2pph2 (dppe), et2p(ch2)2pph2 (depe), and cis-ph2p(ch = ch)pph2 (dppey), are potently cytotoxic to b16 melanoma cells in vitro (ic50 4 microm) and exhibit good activity against ip p388 leukemia in mice. the complex [ag(dppe)2]no3 is active against m5076 reticulum cell sarcoma. the antibacterial and antifungal activities of ag(i) diphosphine and related cu(i) and au(i) complexes were assessed. the complexes [ ...	1988	3139831
suitability of a propanolic pvp-iodophor preparation as preoperative skin disinfectant: germicidal activity and chemical stability.	the preparation solutio betadini propanolica 3% w/w, used as a pre-operative skin disinfectant was examined for its germicidal activity and its chemical stability. the additive activity of iodine and propanol resulted in a wide range of antimicrobial activity against bacteria, including bacterial spores, and fungi, with a rapid onset and sustained duration of action. the solution is easily manufactured and analyzed, its stability is guaranteed for 6 months. because of its staining property, ther ...	1988	3140540
[significance of enterococcus in the development of infectious diseases of the respiratory organs].		1988	3140547
interaction of ciprofloxacin with ampicillin and vancomycin for streptococcus faecalis.	streptococcus faecalis is known to be resistant to killing by many of the commonly used antimicrobial agents. ciprofloxacin, a member of the quinolone class of compounds, was studied for its ability to inhibit and to kill s. faecalis. the rate of killing by ciprofloxacin was slow and was comparable to that of ampicillin or vancomycin. the antimicrobial effect of combining ciprofloxacin with ampicillin or with vancomycin was also studied using checkerboard double-dilution and killing kinetics. th ...	1988	3141108
aortic endocarditis caused by gentamicin-resistant enterococcus faecalis.	a case is reported of a patient who developed aortic endocarditis after protracted hospitalization during which time the patient received multiple courses of aminoglycoside and cephalosporin therapy. the infecting organism, an enterococcus faecalis strain, was resistant to gentamicin and to the combination of ampicillin with gentamicin. valve replacement was performed after four weeks of therapy with ampicillin, and enterococci were isolated from cardiac vegetations. valve replacement may be nec ...	1988	3141162
comparative in vitro activity of cefpiramide, a new parenteral cephalosporin.	the in vitro activity of cefpiramide was compared with that of nine other cephalosporins by determining the mic values by means of an agar dilution method for 300 representative clinical isolates of nonfastidious bacteria. cefpiramide had a broad-spectrum of activity, similar to that of the third-generation cephalosporins. as judged by the mic50 and the mic90 values, cefpiramide was one of the most active cephalosporins against pseudomonas aeruginosa (mic50 2.9 micrograms/ml, mic90 64 micrograms ...	1988	3141169
laboratory evaluation of disposable and reusable disinfectant cloths for cleaning food contact surfaces.	a comparison of five methods of cleaning formica surfaces contaminated with bacteria dried in milk has been carried out. a standardized procedure was developed, and impression plates were found to be at least as sensitive as a swab-rinse method for detecting bacteria on the surfaces. the most satisfactory results were obtained with one type of disposable alcohol-impregnated wipe and with a detergent/hypochlorite solution applied with paper. a reusable cloth impregnated with disinfectant initiall ...	1988	3141202
structural relationships between senfolomycins and paulomycins.	senfolomycins a and b (antimicrob. agents chemother.-1965: 828-831, 1966) are two antibacterial agents with physico-chemical and biological properties similar to those of paulomycin. recent studies indicate that senfolomycin a (c29h36n2o16s, mw 700) has molecular composition and fast atom bombardment ms fragmentation pattern identical to those of paulomycin e. extensive nmr work indicates that the two antibiotics, which have been separated by hplc and tlc, differ only in the stereochemistry of t ...	1988	3141336
synergy between spiramycin and metronidazole in the treatment of polymicrobial infections.	the in-vitro and in-vivo activity of metronidazole and spiramycin, singly or in combination, was tested in the eradication of infection caused by bacteroides spp. alone and in combination with neisseria gonorrhoea, staphylococcus aureus, streptococcus pyogenes or str. faecalis. the in-vitro tests consisted of determinations of the mics with or without the addition of a constant amount of the other antimicrobials. the mics of metronidazole for b. melaninogenicus, b. fragilis and b. bivius were si ...	1988	3141350
serious infection due to beta-lactamase-producing streptococcus faecalis with high-level resistance to gentamicin.		1988	3141522
[an evaluation of the antibacterial effectiveness of bisdequalinium acetate and sodium hypochlorite].	the antimicrobial effectiveness of sodium hypochlorite and bis-dequalinium acetate was evaluated in vitro using three different microorganisms. the solutions were prepared in various concentrations and microorganisms were exposed to these solutions for 5, 10, 15 min. then placed into a culture medium, incubated and determined the presence or absence of growth. these results were compared with those obtained with phenol. the following conclusions were drawn from this study: 1. of the solutions te ...	1987	3141752
[in vitro antibacterial activity of cefpirome in combination with 4 aminoglycosides and 2 fluoroquinolones].	combinations of cefpirome with aminoglycosides (gentamicin, tobramycin, netilmicin, amikacin) and of cefpirome with fluorinated quinolones (pefloxacin, ofloxacin) were tested against 20 enterobacteriaceae, 6 pseudomonas aeruginosa, 6 acinetobacter calcoaceticus, 6 group d streptococci and 6 staphylococcus aureus strains, susceptible, intermediate or resistant with a low level to these different compounds. activity of combinations was evaluated using a broth microdilution checkerboard method and ...	1988	3141888
development of resistance to nalidixic acid and the fluoroquinolones after the introduction of norfloxacin and ofloxacin.	since 1975, the group resistenz of the paul-ehrlich-gesellschaft für chemotherapie has monitored the development of resistance in isolates of members of the family enterobacteriaceae, pseudomonas aeruginosa, staphylococcus aureus, and enterococcus faecalis in the federal republic of germany, west berlin, austria, and switzerland. despite a marked increase in the use of 4-quinolones, there was no increase in the percentages of nalidixic acid-resistant strains of enterobacteriaceae between 1975 an ...	1988	3142353
[substrate specificity of restriction endonuclease sfei].	the recognition sequence and cleavage site c decreases tryag of a new restriction endonuclease sfei have been determined.	1988	3142485
factors affecting transfer frequency of pam beta 1 from streptococcus faecalis to lactobacillus plantarum.	conjugal transfer of streptococcal plasmid pam beta 1 to lactobacillus plantarum by a filter mating method was examined under various conditions. the transfer frequency depended greatly on the type, pore size, and side (front or back) of the membrane filter. the passage of sterilized water through the membrane under reduced pressure after donor and recipient cells were trapped on it increased the transfer frequency about 10-fold.	1988	3142863
[conjugation transfer of the plasmid pambeta1 into various bacillus species].	streptococcal broad host range plasmid pam beta 1 was transferred by a conjugation-like process from streptococcus faecalis to 13 strains of different bacilli species. in intraspecies matings the frequencies of transfer of pam beta 1 varied from 2.10(-5) to 1.10(-8). as it was shown by comparative analysis the frequency of transfer and stability of the maintainance of plasmid pam beta 1 in bacilli were not connected. molecular weight and restriction pattern of pam beta 1 dna isolated from bacill ...	1988	3143066
differential methotrexate sensitivity: a test to distinguish enterococcus faecalis from enterococcus faecium.		1988	3143590
antibiotic resistance and production of inhibitory substances among bacterial strains isolated from the oral cavities of balb/c mice.	in the oral cavities of balb/c mice, microbial population levels are regulated by multifactorial processes. factors include the production of inhibitory substances and the exchange of genetic material. in this work, 371 isolates from different sites (saliva, tongue, teeth, and mucosa) of the oral cavities of balb/c mice were screened for resistance to antibiotics and antimicrobial activity. antibiotic-resistant strains represented 25% of the total flora. among the predominant species, all the s. ...	1988	3143751
in-vitro activity of fleroxacin compared with three other quinolones.	the in-vitro activity of fleroxacin (ro23-6240), a new synthetic quinolone, was compared with that of three other quinolones against 665 clinical isolates of aerobic bacteria by the agar dilution technique. fleroxacin showed similar activity to norfloxacin, enoxacin and pefloxacin against most isolates of enterobacteriaceae, neisseria meningitidis, n. gonorrhoeae, haemophilis influenzae, staphylococcus spp. and streptococcus faecalis. fleroxacin was the most active quinolone against enterobacter ...	1988	3144536
[synthesis and antimicrobial activity of several o-dialkylaminoalkylated adamantane ketoximes and carbohydroxamic acids].		1988	3145691
decrease of urine creatinine in vitro in spinal cord injury patients.	freshly voided urine from 3 spinal cord injury (sci) patients showed a progressive decline in creatinine concentration over 2 weeks while maintained in the refrigerator. urine creatinine concentration remained stable in normals, patients with kidney disease and 9 other sci patients. mixtures of affected and normal urine showed a progressive decline in creatinine. boiling or filtering prevented the decline while light centrifugation enhanced the decrease in the sediment but minimized it in the su ...	1988	3145822
in vitro activity of fce 22101, imipenem, and ceftazidime against over 6,000 bacterial isolates and mic quality control limits of fce 22101.	six geographically separate laboratories within the usa tested 6,198 bacterial isolates against fce 22101 (a penem), imipenem (a carbapenem) and ceftazidime (a third-generation cephalosporin). ninety-three percent of 2,749 enterobacteriaceae were inhibited by fce 22101, while 95% were susceptible to ceftazidime and 99% were susceptible to imipenem. fce 22101 had little activity against pseudomonas spp. but was active against most gram-positive pathogens, including enterococci. fce 22101 mics for ...	1988	3145868
a further insight into the mechanism of action of anthracycline antibiotics.	the uptake of adriamycin (adm) by eucaryotic and procaryotic cells and the interaction of the antibiotic with structurally organized dna were investigated. the aim of this work was to understand why adm is endowed with very low antibiotic activity in spite of being highly cytotoxic for mammalian cells, and to get a further insight into the mechanism of action of this compound. spectrophotometric, fluorometric, chiroptical, and electron microscopic techniques were used. the drug was shown to conc ...	1988	3146012
[prevention using cefotetan of post-partum and post-abortion infectious complications in intra-uterine procedures].	a prospective, randomized study was conducted in 113 women to evaluate the effect of antibiotic prophylaxis with cefotetan versus no prophylaxis in the prevention of post-partum and post-abortion sepsis. the administration of a single 2 g dose of cefotetan at the time of surgery significantly reduced the number of infectious complications, removal of the placenta or an internal inspection were carried out.	1988	3146125
comparative in-vitro activity of erythromycin, vancomycin and pristinamycin.	we have studied the in-vitro activity of erythromycin, vancomycin and pristinamycin against 1,006 clinical isolates comprising streptococci, staphylococci, neisseria gonorrhoeae, haemophilus influenzae and anaerobes. in-vitro studies show pristinamycin to inhibit staphylococci and streptococci, including erythromycin highly-resistant organisms, at a concentration of less than or equal to 0.78 mg/l. although pristinamycin's mean mic for streptococci is higher than that of erythromycin, pristinamy ...	1988	3146552
evidence for the conjugal transfer of the broad host range plasmid pip501 into strains of lactobacillus helveticus.	the conjugative broad host range plasmid pip501 was transferred from streptococcus faecalis to a series of strains of lactic streptococci used commercially as dairy starter cultures. with these transconjugants as donors the plasmid was exconjugated to two strains of lactobacillus helveticus and a commercially used strain of strep. thermophilus. there was evidence that the plasmid could transfer between isogenic derivatives of one of the strains of lact. helveticus. transfer from lact. helveticus ...	1988	3146566
the antibacterial activity of a new 3-azinomethyl-rifamycin.	the new 3-azinomethyl-rifamycin, spa-s-565, was shown to exert an effective antibacterial activity in vitro comparable to that of rifampicin. in fact, the antibacterial activity of spa-s-565 against numerous gram-positive cocci belonging to staphylococcus and streptococcus species as well as against 20 strains of mycobacterium tuberculosis, was similar to that of rifampicin. in swiss albino mice intraperitoneally infected with staphylococcus aureus oxford strain or streptococcus pyogenes, the pr ...	1988	3147199
[evaluation of the effect of biologically purified waste water on the survival of microflora].		1988	3147225
[study of bacterial contamination of peloid in the process of its use and in its formation site].		1988	3147226
[study of the pathogenicity of enterococcus faecalis--experimental mice with mixed infection with e. faecalis and other organisms].		1988	3147302
laboratory detection of high-level aminoglycoside-aminocyclitol resistance in enterococcus spp.	methods for detection of high-level resistance to aminoglycoside-aminocyclitol antibiotics were evaluated using 104 blood isolates of enterococci (97 enterococcus faecalis and 7 enterococcus faecium). kanamycin was used to predict resistance to amikacin. discrepancies between methods were resolved by time-kill studies. four methods (microscan, macrotube, microtiter, and disk diffusion) for detecting resistance to gentamicin and streptomycin were compared, using 51 consecutive strains. there were ...	1988	3148633
survival of some non-starter bacteria in naturally ripened and enzyme-accelerated cheddar cheese.	effects of the addition of a proteinase (neutrase 1-5s) and a peptidase (aminopeptidase dp-102) as agents for accelerating the ripening of cheddar cheese on the survival of some non-starter bacteria (staphylococcus aureus, enterococcus faecalis, escherichia coli and a salmonella sp.) were studied throughout a 4-month ripening period. the enzymes were found to have no significant effect on the survival of the gram-positive bacteria but some significant effects were observed, at some stages of the ...	1988	3148643
detection of a large haemolysin plasmid carrying multiple antibiotic resistance markers in streptococcus faecalis.		1987	3148809
streptococcus faecalis susceptibility to amiloride depends on medium ph.	amiloride is one of the major molecular probes in basic and applied investigations on the physiology of cation transport in animal cells. in these cells the drug also exerts growth inhibitory activity. recently, we discovered that amiloride causes growth inhibition also on bacterial cells. in this paper we report that medium ph influences amiloride activity on streptococcus faecalis. the lowering of external ph causes a drop in the susceptibility of this bacterium to amiloride up to an almost co ...	1988	3148937
characterization of beta-lactam antagonist in supplement c.	in the course of using mueller-hinton agar with 1% supplement c (sc) (difco, detroit, mi) as a susceptibility test medium for haemophilus influenzae, one lot of sc was encountered whose use was associated with markedly increased ampicillin mics. acidimetric and chromogenic cephalosporin filter paper disc tests of sc failed to detect beta-lactamase activity. macrobroth dilution mic tests to determine substrate specificity showed sc to antagonize benzylpenicillin and ampicillin but not cephalothin ...	1988	3149566
bactericidal effect and regrowth of streptococcus faecalis exposed to amoxicillin following beta-lactamase.	streptococcus faecalis usually requires high concentrations of penicillin or ampicillin to achieve killing (i.e. a high mbc/mic ratio). however, most strains show the eagle or paradoxical effect. we subjected 12 strains of s. faecalis to 0.1, 1, 10 and 100 micrograms/ml of amoxicillin. turbidometry studies have shown that 3 h after the inactivation of amoxicillin by penicillinase, there was a longer effect for 1 micrograms/ml following beta-lactamase (12 h 31 min +/- 2 h 09 min) than for 10 micr ...	1988	3149569
a medium for the detection of lancefield group d cocci in skimmed milk powder by measurement of conductivity changes.	a selective medium for the detection of lancefield group d cocci in skimmed milk powder by conductivity measurements was developed and evaluated using the bactometer m123 and malthus 128h systems. this medium promoted large changes in conductance and capacitance. the calibration curve of detection times vs concentration of lancefield group d cocci showed a linear correlation coefficient of 0.93 and the method gave comparable results in both conductivity instruments. naturally contaminated sample ...	1988	3149630
activity of amikacin and ampicillin in succession and in combination.	one strain each of escherichia coli and streptococcus faecalis were exposed to amikacin and ampicillin in combination as well as in succession. exposure to ampicillin for 1 hr followed by amikacin for 3 or 4 hr had the greatest antibacterial activity when the antibiotics were applied in succession. the least effective exposures for both organisms were 1 hr to amikacin followed by 3 or 4 hr to ampicillin. exposure to the antibiotics in combination each at 1 mic had the overall greatest antibacter ...	1988	3149928
[purulent pericarditis, pleural effusion and acute arterial occlusion of a lower limb].		1988	3150030
[effect of streptococci on epithelial cells of the vagina of albino inbred mice nmri].		1988	3150037
chemotactic and chemokinetic activity of streptococcus faecalis culture supernatant for equine neutrophils.	although equine neutrophils did not respond towards formylated methionyl peptides, streptococcus faecalis culture supernatant caused an in vitro stimulation of equine neutrophil motility when measured by an under-agarose assay. the migration of neutrophils towards the culture supernatant increased sigmoidally with the logarithmic concentration of the culture supernatant in the chemoattractant wells. the streptococcal culture supernatant was chemokinetic because it stimulated the random motility ...	1988	3150886
[venous and capillary diffusion of enterococcus faecalis during experimental bacteremia in the chicken].	capillary and venous diffusion of bacteria were studied in chickens after intravenous inoculation with a strain of enterococcus faecalis isolated from a case of septicaemia in a human neonate. one group of 13 chickens was inoculated with 10(8) bacteria/animal and another group of 20 chickens with 10(7) bacteria/animal. the level of bacteraemia in the first group of chickens was similar in capillary and venous blood. with 10(8) e. faecalis, the clearance of bacteria was very weak. the level of ba ...	1988	3150938
[surgical treatment of complications of acute pancreatitis including the use of ceftazidime (fortum)].		1988	3151031
incidence, clinical distribution, and susceptibility to several antibiotics of enterococci isolated in some italian regions.		1987	3151343
different activity of penicillins and cephalosporins on enterococci.		1987	3151354
effects of single-dose ceftriaxone on intestinal flora.		1987	3151359
[calcium hydroxide cement. verification of the influence of contact time on antimicrobial action against s. faecalis. in vitro study].		1988	3152056
[the use of granulated sugar in the treatment of anterior mediastinitis].		1988	3152768
histamine formation by enterococci isolated from home-made goat cheeses.	a survey was made of the histamine-producing capability of enterococci isolated from goat cheeses. the strains, 130 streptococcus faecium and 106 s. faecalis, were grown in trypticase soy broth histidine medium (tsbh) at 35 degrees c for 24 h and the histamine formed was determined by fluorometry. forty-one (31.5%) of the s. faecium strains and 2 (1.9%) of the s. faecalis strains produced histamine. the largest amount detected was 4.0 micrograms histamine/ml tsbh. compared with the amounts of hi ...	1988	3152802
formation of a beta-aspartyl phosphate intermediate by the vanadate-sensitive atpase of streptococcus faecalis.	the vanadate-sensitive membrane atpase of streptococcus faecalis forms, as part of the reaction cycle, an acylphosphate intermediate. the phosphorylated amino acid residue was identified by reducing the purified reconstituted phosphoenzyme with [3h]borohydride, followed by acid hydrolysis of the protein and quantitative amino acid analysis. tritiated homoserine was found to be the resulting reaction product, generated through the reduction of a beta-aspartyl phosphate residue. the s. faecalis at ...	1985	3155519
bactericidal effects of photoradiation therapy with hematoporphyrin derivative.	hematoporphyrin derivative (hpd) localizes selectively in malignant and rapidly metabolizing tissues and undergoes a cytotoxic reaction when exposed to light of a specific wavelength. hpd has been studied extensively with regard to the diagnosis and treatment of tumors but not with regard to bactericidal activity. this investigation assessed the effect of light-activated hpd on various microorganisms, on human polymorphonuclear leukocytes, and on the interactions of polymorphonuclear leukocytes ...	1985	3155547
comparative evaluation of recently developed quinolone compounds--with a note on the frequency of resistant mutants.	the antibacterial activity of the new quinolone compounds enoxacin, norfloxacin, ofloxacin and ciprofloxacin was evaluated in 300 enterobacteriaceae, 50 pseudomonas aeruginosa, 30 acinetobacter spp., 15 haemophilus influenzae, 50 streptococcus faecalis, and 70 staphylococcus aureus isolates and compared to that of nalidixic acid, gentamicin and various beta-lactam compounds. moreover, the rate of spontaneous mutants resistant to quinolone compounds was evaluated. in concentrations only insignifi ...	1985	3156025
laboratory evaluation of enoxacin: comparison with norfloxacin and nalidixic acid.	enoxacin displayed activity similar to that of norfloxacin against enterobacteria, pseudomonas aeruginosa, staphylococci, streptococci and bacteroides spp. the activity of enoxacin against many strains was reduced in acid conditions, but the ph effect was not so marked as that seen with norfloxacin. nalidixic acid was found to be more active in acid conditions, particularly against staphylococci, streptococcus faecalis and ps. aeruginosa. in conditions simulating the treatment of bacterial cysti ...	1985	3156113
therapeutic efficacy of teicoplanin in experimental enterococcal endocarditis.	the antimicrobial activities of teicoplanin and ampicillin, alone and in combination with gentamicin, were compared in experimental streptococcus faecalis endocarditis. bacterial titers in vegetations of rabbits treated with teicoplanin were significantly lower than those of untreated controls (p less than 0.01) and were equivalent to titers in ampicillin-treated animals. gentamicin increased the activities of both drugs to a comparable degree.	1985	3157344
in vitro activity of six antibiotics against multiresistant staphylococci and other gram-positive cocci.	sixty-two strains of staphylococcus aureus and coagulase-negative staphylococci, 21 streptococcus faecalis and 17 other strains of streptococci isolated from cases of endocarditis were tested for sensitivity against rifampicin, teicoplanin, vancomycin, fusidic acid, erythromycin and novobiocin. only rifampicin, novobiocin and teicoplanin were found to be active against the great majority of these strains. the microbial properties of these antibiotics suggest the necessity of combinations for eff ...	1985	3157567
in vitro activity of enoxacin compared with norfloxacin and amikacin.	the in vitro activity of enoxacin was tested against 1000 clinical isolates of gram-positive and gram-negative microorganisms and compared with that of norfloxacin and amikacin. the mic90 of enoxacin was 1 mg/l for enterobacteriaceae, 2.6 mg/l for pseudomonas aeruginosa and 6.2 mg/l or staphylococcus aureus. the inoculum effect was minimal. the mics and mbcs for pseudomonas aeruginosa strains were significantly affected by the addition of calcium and magnesium ions. synergy was occasionally obse ...	1985	3157568
the pharmacokinetics and serum and urine bactericidal activity of ciprofloxacin.	ciprofloxacin is an investigational quinolone agent possessing an impressive antibacterial spectrum. its pharmacokinetics were studied in six volunteers after 250-mg and 500-mg single oral doses, and its bactericidal activity compared to that of trimethoprim-sulfamethoxazole given to the same volunteers. mean peak serum levels were 1.45 micrograms/ml and 2.46 micrograms/ml for 250-mg and 500-mg doses, and time to peak was 1 and 1.3 hours. the 12-hour levels were 0.12 micrograms and 0.22 microgra ...	1985	3157705
[susceptibilities of clinical isolates to antibacterial agents. focusing mainly on ofloxacin (first report). reported by the research group for testing ofloxacin susceptibility of clinical isolates].	susceptibility tests were carried out on a variety of clinically isolated pathogens using the susceptibility disc method at 197 hospitals in japan between may, 1985 through march, 1986. these tests were organized by the research group for testing ofloxacin susceptibility on clinical isolates, and the results were statistically analyzed. this paper describes a comparison of susceptibilities of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, st ...	1988	3163383
in-vitro adherence of oral streptococci in the presence of sucrose and its relationship to cariogenicity in the rat.	streptococcus sanguis i gave a significantly greater percentage coverage (cell-pellicle attachment) of saliva-coated glass in the presence of sucrose than did strep. sanguis ii (p less than 0.025), and both these gave greater percentage coverages than the other species tested, between which no significant differences were noted. there was a large number of significant differences in clump size (cell-cell attachment) between species-pairs. among the mutans streptococci, there were significant dif ...	1988	3166609
reversal of activity of trimethoprim against gram-positive cocci by thymidine, thymine and 'folates'.	the reversal of the antibacterial activity of trimethoprim against different species of gram-positive cocci (staphylococcus aureus, staph. epidermidis, staph. saprophyticus, group b streptococci, streptococcus faecalis and str. faecium) by thymine, thymidine and various 'folates' (folate, folinate, dihydrofolate and tetrahydrofolate) was tested. against group b streptococci and staphylococci only thymidine antagonized trimethoprim. however, for enterococci, thymine, thymidine, dihydrofolate, tet ...	1988	3170389
glycyrrhizin stimulates growth of eubacterium sp. strain glh, a human intestinal anaerobe.	eubacterium sp. strain glh was isolated from human feces and produced two kinds of beta-d-glucuronidase (ec 3.2.1.31), one new enzyme specific for glycyrrhizin (gl) and the other for phenyl beta-d-glucuronides. gl or p-nitrophenyl-mono-beta-d-glucuronide (pnpg) stimulated the production of gl or pnpg beta-glucuronidases and the growth of strain glh in a basal medium lacking carbohydrate. d-glucuronic acid also stimulated the growth of the bacterium, but glycyrrhetic acid did not. the increase of ...	1988	3178209
ciprofloxacin in combination with metronidazole.	ciprofloxacin has a reduced activity against anaerobic pathogens. therefore, a combination of ciprofloxacin with an antimicrobial agent active against anaerobes, such as metronidazole, seems to be interesting for the treatment of mixed aerobic/anaerobic infections. high metronidazole concentrations (10 mg/l or 40 mg/l) neither affected the bactericidal efficacy of ciprofloxacin on aerobic pathogens, such as escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and enterococcus faecalis ...	1988	3182096
in vitro activity of u-76,252 (cs-807), a new oral cephalosporin.	u-76,252 is the prodrug of u-76,253. mics of u-76,253 were 0.015 to 0.06 microgram/ml for greater than or equal to 90% of the strains of streptococcus spp., haemophilus influenzae, and proteus mirabilis; 0.25 to 1 microgram/ml for branhamella catarrhalis, escherichia coli, klebsiella spp., and citrobacter diversus; 1 to 8 micrograms/ml for staphylococcus spp.; and 2 to greater than 16 micrograms/ml for other members of the family enterobacteriaceae and aeromonas hydrophila; for 72% of the latter ...	1988	3190194
effect of excimer laser on microbiological organisms.	the effect of radiation emitted from an excimer laser filled with argon fluoride gas at 193 nm on serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus, streptococcus faecalis, hemophilus influenzae, candida albicans, and aspergillus niger (collectively labeled "the microorganisms") was examined. colonies were subjected to a variable number of radiation pulses from the excimer laser applied after a 36-hour period of incubation at 37 degrees c, at which time the colonies were fully g ...	1988	3194107
[clinical laboratory approach for estimating effective administrative dose of cefoperazone. evaluation of disc susceptibility test and its interpretation system].	to interpret of the cefoperazone (cpz) disc susceptibility test, a 4 category system is used in japan, but a 3 category system is used in the u.s.a. and europe. in the 4 category interpretation system of showa cpz disc the following classification is used: ( ) mic less than or equal to 3 micrograms/ml, (++) mic greater than 3 approximately 15 micrograms/ml, (+) mic greater than 15 approximately 60 micrograms/ml, (-) mic greater than 60 micrograms/ml. in the 3 category system the classification u ...	1988	3204655
in vivo antibacterial activity of fk482, a new orally active cephalosporin.	the therapeutic activities of orally administered fk482 were compared with those of reference antibiotics against systemic and local infections with a variety of bacteria in mice and rabbits. in systemic infections in mice, oral fk482 was almost as effective as oral cefaclor (ccl) and more effective than oral cephalexin (cex) against staphylococcus aureus, escherichia coli, klebsiella pneumoniae and proteus mirabilis infections. however, fk482 afforded superior protective activity when given sub ...	1988	3209480
[evaluation of imipenem/cilastatin sodium in neonatal infections].	ten patients with infections (8 neonates and 2 infants) were treated with 10.2 mg/10.2 mg/kg-37.7 mg/37.7 mg/kg of imipenem/cilastatin sodium (ipm/cs) b.i.d. or t.i.d. by a 1-hour intravenous drip infusion. the plasma concentrations of ipm/cs were determined in 5 of the 10 patients and in the cerebrospinal fluid of 1 patient of the 5. 1. the patients studied included 5 with pneumonia and 1 each with urinary tract infection, omphalitis, suspected meningitis, periproctal abscess and suspected sept ...	1988	3210303
[current resistance status of pathogens isolated from bile and its development since 1976 in the suhl district].	bile was collected intraoperatively and through duodenal tubes from patients and was microbiologically investigated. e. coli and coliform bacteria were found to be the most common germs in bile, accounting for 51.6 per cent of all positive findings. streptococcus faecalis and proteus spec. increased remarkably to 17 per cent each. calculated antibiotic therapy was found to depend for good success on knowledge of the latest resistance rates of the most common germs. these were 67 per cent e. coli ...	1988	3213295
[use of ceftriaxone against parametritis after radical surgery for cancer of the uterine cervix].	1. the bacteria isolated from pelvic dead space drainage well reflect the characteristics of prophylactic drugs. as drugs advance from penicillin (pc) to tetracycline (tc) to the first, second and third generation cephems, detection rates for gram-negative bacilli, mainly escherichia coli, decrease remarkably, while those for gram-positive cocci, mainly enterococcus faecalis, increase. 2. the bacteria isolated from the drainage and those which later caused parametritis and purulent lymphocyst we ...	1988	3216481
partial characterisation of the inhibitory substances produced by streptococcus oralis and related species.	the production of inhibitory substances was sought by deferred antagonism. streptococcus oralis, streptococcus mitis and streptococcus sanguis produced bactericidal concentrations of hydrogen peroxide, but no detectable bacteriocin. actinomycetes and neisseria pharyngis were particularly susceptible to the lethal action of hydrogen peroxide. streptococcus milleri produced bactericidal concentrations of hydrogen peroxide and bacteriocin-like substances. s. oralis strains were inhibited by the bac ...	1988	3216798
in vitro activity of cefixime, a new oral cephalosporin.	the in vitro activities of cefixime and seven comparative oral antimicrobials were studied. mic90s of cefixime were 0.015-1 micrograms/ml for haemophilus influenzae, branhamella catarrhalis, escherichia coli, klebsiella, citrobacter diversus, proteus, providencia, aeromonas hydrophila and streptococcus. mic90s for other enterobacteriaceae were greater than or equal to 2 micrograms/ml; 44% of those strains were inhibited by less than or equal 1 microgram/ml. staphylococcus, psuedomonas aeruginosa ...	1988	3219746
an in vitro evaluation of hre 664, a novel penem antibiotic.	hre 664 is a novel parenteral penem with a broad antimicrobial spectrum. in this study, hre 664 inhibited 90% of enterobacteriaceae at less than or equal to 2.0 micrograms/ml, haemophilus influenzae and neisseria gonorrhoeae at less than or equal to 0.5 microgram/ml, oxacillin-susceptible staphylococci at less than or equal to 0.13 microgram/ml, oxacillin-resistant staphylococci at less than or equal to 8.0 micrograms/ml, enterococci at less than or equal to 8.0 micrograms/ml, and streptococci a ...	1988	3219818
[clinical laboratory approach in estimating the effective dosage of cefamandole].	reliability of the cefamandole (cmd) disc susceptibility test in estimating approximate values of mics was studied using various clinical isolates totaling 246 strains with showa discs (8 mm diameter containing 30 micrograms of cmd). clinical significance of a 4 category system for the interpretation of the cmd disc tests, which is normally used in japan, was also evaluated to determine whether this system would be suitable or not for the evaluation of a proper dose of administration. the result ...	1988	3221428
the effect of decreased portal blood flow on the biliary system.	a disturbance in the regional portal blood flow adjacent to the dilated bile duct in the liver is often observed in patients with hepatolithiasis. the effects of this disturbed portal blood flow on the biliary system, with or without cholangitis, were therefore investigated. young rabbits were divided into the following four groups; (1) controls that had a laparotomy only (n = 3), (2) those that had a ligation of the portal branch of the right posterior lobe (rp lobe) (pl) (n = 10), (3) those th ...	1988	3230727
[experimental polymicrobial osteomyelitis produced by both aerobic and anaerobic opportunistic pathogens].	experimental models of polymicrobial osteomyelitis were prepared using clinical isolates of staphylococcus epidermidis and enterococcus faecalis as aerobes and bacteroides fragilis and bacteroides bivius as anaerobes. these pathogens were used because of their opportunistic properties. two 8 mm long silk threads with the microorganism were inserted into the bone marrow of a rat. the microorganism inoculated was about 10(5) c.f.u. for aerobes and 10(6) c.f.u. for anaerobes. infected parts observe ...	1988	3235897
pharmacokinetics of leucovorin calcium after intravenous, intramuscular, and oral administration.	the pharmacokinetics of leucovorin was evaluated after intravenous, intramuscular, and oral administration in a randomized crossover study of 37 healthy men. a single 25-mg dose of leucovorin calcium was administered intravenously, intramuscularly, or orally to the subjects. blood samples were obtained immediately before and at 13 time points up to 24 hours after the leucovorin dose. the three treatment phases were separated by one-week intervals. bioavailability was assessed by measuring over 2 ...	1988	3257913
in vitro antibacterial activity of fk482, a new orally active cephalosporin.	fk482 is a new orally active cephem antibiotic which offers some advantages over the commercially available oral beta-lactam antibiotics. it displayed a broad spectrum of activity in vitro against stock strains of gram-positive and gram-negative aerobes and anaerobes. fk482 was more active in vitro than cefixime (cfix), cefaclor (ccl) or cephalexin (cex) against clinical isolates of gram-positive organisms such as methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococci incl ...	1988	3264828
[influence of sultamicillin on intestinal bacterial flora].	effects of sultamicillin (sbtpc) fine granules, a new oral beta-lactam antibiotic, on the intestinal bacterial flora were studied in tetra-contaminated mice and in pediatric patients. sbtpc was administered at a dose of 100 mg/kg once a day for 5 consecutive days to mice contaminated with 4 different species of organisms: escherichia coli, enterococcus faecalis, bacteroides fragilis and bifidobacterium breve. in all of the 4 species, bacterial populations in feces were markedly reduced on days 4 ...	1988	3266882
[urine bacterial flora and chemoantibiotic resistance: observations on ambulatory patients and hospitalized patients at the local health district no. 11 of fabriano (ancona)].	this paper reports the results of a study carried out on 4614 samples of urine from inpatients and outpatients according to the sex. the positive monomicrobic urine specimens were examined and isolated strains were tested against various antimicrobial drugs. our findings showed that 43.1% of urine specimens examined from inpatients and 38.4% from outpatients proved to be positive. among the bacterial species more frequently isolated, significant differences among enterobacter spp. in males and c ...	1988	3268917