Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: structure and functions of the rfb gene cluster. | the genetic organization and functions of the shigella dysenteriae 1 rfb gene cluster, which specifies the somatic o antigen in this organism, have been studied in escherichia coli k-12 by insertion and deletion mutagenesis of pss9, a pbr322 hybrid containing the shigella rfb genes. on the basis of the sensitivity/resistance to rough-specific bacteriophage t3 of e. coli k-12 derivatives containing mutant pss9 plasmids, of the banding patterns and immunoreactivity of lps isolated from such deriva ... | 1986 | 2469933 |
| human milk immunoglobulin a antibodies to shigella virulence determinants. | because human milk is thought to protect infants from shigellosis, we evaluated milk for immunoglobulin a to shigella virulence determinants. milk was preincubated to remove antibodies unrelated to each locus of interest, using defined shigella and e. coli hybrids containing known shigella genetic segments prior to immunoblotting. the milk could not be shown to contain antibodies to chromosomally encoded virulence loci except for the expected antibodies to the products of the histidine locus. ho ... | 1989 | 2470676 |
| in vitro treatment of hep-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of salmonella typhimurium. | enteroinvasive bacteria, like salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like hep-2 cells. this phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (tnf-alpha) in a dose- and time-dependent manner. the effect was also reproduced in other cell types, including diploid embryo fibroblast cells. the tnf-alpha effect was neutralized by anti-tnf-alpha antibodies. no synergistic effect was produced by combinations of tnf-alp ... | 1989 | 2472042 |
| [a comparison of the sorption capability and standardization of plates of different shapes for immunoenzyme analysis]. | the sorption characteristics of elisa plates manufactured by the moscow and leningrad plants and by some foreign firms are compared. sorption activities and standard specifications of the plates for chemically heterogeneous antigens like ovalbumin or shigella sonnei lipopolysaccharides and for complex antigens like mycobacterium tuberculosis or s. sonnei ribosomal fractions have been examined. sorption activities of the plates varied in tests with different antigens. the sorption activity and th ... | 1989 | 2473268 |
| expression in escherichia coli k-12 of the 76,000-dalton iron-regulated outer membrane protein of shigella flexneri confers sensitivity to cloacin df13 in the absence of shigella o antigen. | one of the chromosomal segments associated with virulence in shigella flexneri encodes the production of aerobactin and the synthesis of an iron-regulated 76-kilodalton outer membrane protein believed to be the ferric-aerobactin receptor. however, s. flexneri expressing this putative aerobactin receptor, which is slightly larger than that encoded by pcolv, is insensitive to the killing action of cloacin df13, a bacteriocin which binds to other aerobactin receptor proteins and kills the cells. in ... | 1989 | 2474501 |
| a dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of shigella flexneri. | the expression of plasmid-encoded, invasion-related antigens lpa b, c and d of shigella flexneri was found to be positively regulated at transcriptional level by a 33kd protein produced by the previously defined, virulence-associated region 1 on the sali fragment b of the 230 kb invasion plasmid. the gene (designated virb) was identified and its nucleotide sequence determined. no ipa b or c was produced in the absence of an intact virb gene although lower levels of d were produced. the previousl ... | 1989 | 2474742 |
| identification of a 2-md plasmid from shigella flexneri associated with reactive arthritis. | the development of reactive arthritis, a sterile inflammatory polyarthropathy that primarily affects hla-b27 positive individuals, has been associated with previous enteric infections caused by various gram-negative bacteria. the possibility that a common bacterial epitope triggers the disease was investigated by screening a panel of documented arthritogenic shigella strains as well as 2 epidemic-associated nonarthritogenic shigella controls. a 2-md plasmid specific to the arthritogenic strains ... | 1989 | 2475114 |
| [development and use of solid-phase test-systems for the detection of o-antigen of shigella sonnei and shigella flexneri]. | the possibility of the diagnosis of dysentery caused by s. sonnei and s. flexneri, as well as the determination of the dynamics of the distribution of specific o-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. the study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, thes ... | 1989 | 2477970 |
| [use of immunoenzyme analysis in the rapid diagnosis and study of the characteristics of circulating o-antigen in biological fluids of patients with acute dysentery sonne]. | the data on the clinical approval of the original enzyme immunoassay system for the determination of somatic o-antigen in the blood serum and urine of patients with acute sonne dysentery are presented. the level of the antigen determined in the biological fluids of patients has been shown to depend on the severity of the disease. different types of dynamic curves, reflecting the level of o-antigen in the biological fluids of patients with acute sonne dysentery and characteristic of different cli ... | 1989 | 2477971 |
| production and characterisation of monoclonal antibodies to verotoxins 1 and 2 from escherichia coli of serotype o 157:h7. | fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (mabs) to purified verotoxins 1 and 2 (vt1, vt2) of escherichia coli of serotype o157:h7. of these mabs, eight were obtained by immunisation of balb/c mice with purified vt1, and six were obtained from balb/c mice immunised with purified vt2. with the exception of mab 1c5, with a heavy chain of igg2b class, antibodies produced from mice immunised with heat-treated toxin were of igm class. mabs produced from mice immu ... | 1989 | 2479749 |
| [an immunoenzyme test-system for detecting the o-antigen to sonne's shigella]. | 1988 | 2479999 | |
| [use of an immunoenzyme test-system for detecting the o-antigen to sonne's shigella in clinical practice]. | 1988 | 2480000 | |
| [an immunoenzyme method for determining the o-antigen of sonne's shigella with the use of affinity isolated antibodies]. | the sensitivities of elisa systems for assays of shigella sonnei o-antigen, developed on the basis of specific rabbit antibodies isolated from antisera by different chromatography techniques have been compared. the highest specificity and sensitivity of o-antigen determination (0.5-1 ng/ml) is achieved when using affinity-isolated antibodies to o-antigen. clinical trials of this elisa system have been carried out. | 1989 | 2481140 |
| aetiological factors of infantile diarrhoea: a community-based study. | a community-based study was undertaken to compare the organisms responsible for diarrhoea in children living in formal housing with indoor water supply and sanitation with those from a deprived environment. the role of "home remedies" was also assessed. among 373 children with diarrhoea, rotavirus was detected in 15% (in 371 symptom-free controls, 9%), and proved to be the single most common causative agent. bacterial pathogens were found in 20% of patients, with enteropathogenic escherichia col ... | 1989 | 2482008 |
| [o-specific l-hapten in the composition of shigella sonnei]. | along with classical lipopolysaccharide (lps), o-specific material not precipitated by ultracentrifugation has been isolated from the water-phenol extract of s. sonnei avirulent strain 9090 possessing complete antigenic properties. the purification of o-antigen contained in the supernatant fluid has been carried out by the gel filtration of the fluid, previously treated with ribonuclease, in a column packed with sephadex g-100. the polysaccharide nature of o-antigen thus obtained, the absence of ... | 1989 | 2482642 |
| effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins. | the interferon (ifn)-inducing activity of detoxified lipopolysaccharide (lps) was tested in rabbits treated with lps preparation derived from escherichia coli, salmonella typhi, salmonella enteritidis and shigella dysenteriae serovar 1. of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the ifn-inducing potential of lps. in contrast, acet ... | 1989 | 2484274 |
| [the fate of pathogens in waste-waters]. | a large variety of pathogenic organisms capable of transmission by the faecal-oral route may be found in wastewaters. among the bacteria salmonella, shigella, vibrio cholerae, yersinia and campylobacter are the important agents of concern. also the human enteric viruses (poliovirus, coxsackievirus, echovirus, hepatitis a virus, rotavirus) have been shown to be present in domestic waste and may not be completely removed by conventional sewage treatment processes, including chlorination. discharge ... | 1989 | 2484470 |
| molecular genetics of virulence in shigella. | the application of modern genetic techniques to shigella is revealing many interesting aspects of the genetics of virulence in these organisms. the information obtained allows rational approaches towards vaccine development to be investigated. | 1985 | 2484669 |
| studies on shiga-like toxin produced by enterohemorrhagic escherichia coli: purification and characterization of the toxin and development of methods for identifying the toxin. | a simple purification method using deae cellulose column chromatography and immunoaffinity column chromatography was developed for purifying shiga-like toxin produced by escherichia coli o157:h7. about 0.75 mg of purified toxin was obtained from 5 liters of culture (62% recovery). the purified toxin was demonstrated to be immunologically, biologically and structurally indistinguishable from shiga toxin. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga-lik ... | 1989 | 2485395 |
| [cloning of the gene relative to invasive ability of shigella flexneri 5]. | a genomic library of s. flexneri 5 large plasmid (140 md) was constructed using the cosmid pjb8 as vector. there were more than 4,000 transformants in the library. 66 clones were picked out from the library by hybridization with 17kb gene probe relative to invasive ability. a few clones were analyzed. the results indicated that all these clones tested contained recombinant plasmids, they could hybridize with 17 kb gene probe. when these recombinant plasmids were digested with ecor1, 17 kb fragme ... | 1989 | 2486252 |
| [microbiological quality of vanilla ice cream manufactured in caracas, venezuela]. | a total of 122 samples of vanilla ice cream, the base product used for all flavors, prepared by eight different large firms at the metropolitan area of caracas, venezuela, were analyzed for aerobic mesophilic and psicrophilic bacteria, staphylococcus aureus, enterobacteriaceae, and filamentous fungi. findings revealed that within the sampling, 56.6% complied with the international standards proposed for aerobic mesophilic bacteria, 68% for staphylococcus aureus, and 23% for enterobacteriaceae. t ... | 1989 | 2487021 |
| mechanism of antigenic variation in shigella flexneri bacilli. i. selective lethal effect of normal sera on mixed population of s. flexneri 1b serotype and its antigenic 3b mutant. | normal non-immune calf, human and mice sera kill-preferentially the shigella flexneri 1b serotype cells with antigenic formula i; 3, 4, 6 leaving the antigenic mutant cells of the serotype 3b with antigenic formula iii; 3, 4, 6. the strongest lethal action was observed in normal calf serum. this serum makes possible to obtain the 3b serotypes mutant from pure population of 1b serotype cells. thermal inactivation of the complement deprives the serum of its selective activity. | 1989 | 2487379 |
| [in vitro activity of various antibiotics against shigella strains]. | the in-vitro activity of ampicillin, chloramphenicol, oxytetracycline, trimethoprim-sulfamethaxazole (tmp-smx), cefoperazone, ceftriaxone, cefotaxime, ceftizoxime, ofloxacin, pefloxacin, ciprofloxacin and fleroxacin against clinically isolated strains of shigella spp. (n 42) have been determined in this study. the minimum inhibitory concentrations (mics) were established using the microtube dilution technique. in shigella strains relatively high percentage of resistance was found against ampicil ... | 1989 | 2487462 |
| [microbiological quality control of lipsticks which are on the market in our country]. | in this study, microbiological quality control of lipsticks has been investigated. in 81 samples, we found that 34 of them (42%) had total aerobic plate count and 19 of them (23.5%) were found to consist mold and yeast which are not allowed by the cosmetic regulations. in none of the samples, pathogen microorganisms such as s. aureus, p. aeruginosa, e.coli, salmonella and shigella were detected. indeed, after carefully examining of the microbiological content of these lipsticks, their biostatist ... | 1989 | 2488946 |
| [multicenter study on bacterial gastroenteritis in regional hospitals of the province of barcelona. group of microbiologists of the regional hospitals of catalonia]. | a retrospective analysis of 26,901 coprocultures is reported. of these, in 5,847 (21.7%) an enteric pathogen was isolated. the positivity rate in adults was 21.0%, and 22.1% in children. statistically significant differences (p less than 0.001) were found in the number of overall isolates throughout the years for both age groups. the most common enteropathogen was salmonella enterica spp i non typhi (59.4% of the isolates in children and 76.9% in adults). the rate of isolation of campylobacter s ... | 1989 | 2490674 |
| [increase of muscle enzymes in shigella sonnei infection]. | 1989 | 2491196 | |
| method for the detection of shigella in foods. | a method for the detection of shigella in foods was evaluated. the method consisted of selective enrichment at 37 degrees c in gn-broth supplemented with novobiocin (10 micrograms/ml) followed by subculture after 6 and 24 h incubation of 0.1 ml volumes to ss-agar with and without streptomycin (7.5 micrograms/ml). test samples were contaminated by adding shigellae to 1:10 suspensions of foods (minced meat, fresh cut vegetables and cooked peeled shrimp) in the gn-broth with novobiocin. shigella co ... | 1989 | 2494820 |
| convulsions associated with shigellosis in children. | 1989 | 2497628 | |
| four new provisional serovars of shigella. | four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ... | 1989 | 2501349 |
| [drug-resistance and its transferability of shigella strains isolated in 1986 in japan]. | two hundred and fifty shigella strains isolated in japan were examined for their drug-resistances and their conjugal transferability. drugs used and the isolation frequency of resistant strains were: sulfanilamide (sa) 96%, streptomycin (sm) 85%, tetracycline (tc) 82%, fosfomycin (fom) 76%, chloramphenicol (cp) 51%, trimethoprim (tmp) 46%, ampicillin (abpc) 45%, nalidixic acid (na) 6%, kanamycin (km) 5%, cephaloridine (cer) 4%, gentamicin (gm) 0%, amikacin (amk) 0%, colistin (cl) 0%, norfloxacin ... | 1989 | 2501419 |
| [value of determining the spectrum of blood serum fatty acids in evaluating increasing the effectiveness of antibiotic therapy of dysentery in children with prodigiozan and ephedrine]. | one hundred and ninety one children with acute sonne and flexner dysentery were observed with respect to the disease process, immunity indices and blood serum fatty acid spectrum. 104 children were treated with monomycin alone and 87 children were treated with the antibiotic in combination with prodigiozan and ephedrine as immunostimulators. it was shown that the recovery terms in the patients treated with the use of the immunostimulators decreased as compared to the patients treated with the an ... | 1989 | 2502081 |
| hemagglutinating properties of shigella dysenteriae type 1 and other shigella species. | strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ... | 1989 | 2503449 |
| infections in british clinical laboratories, 1986-87. | during 1986-87 this continuing survey showed 15 specific infections in the staff of 235 laboratories, representing 28,524 person years of exposure. the community was the probable source of four of the five cases of tuberculosis and one of the five cases of salmonellosis. occupational exposure was the probable cause of four infections by shigella flexneri, three by salmonella typhimurium, and one by s typhi, all affecting medical laboratory scientific officers (mlsos) in microbiology. occupationa ... | 1989 | 2503546 |
| surveillance of antibiotic resistance in south east asia. | antibiotic resistance in gram-negative bacteria, particularly salmonella and shigella, requires surveillance worldwide. this study describes results of surveys in hong kong, bangkok and kuala lumpur. all strains were isolated in hospitals which have large community catchment areas in addition to specialised hospital units. the prevalence of resistant strains was high in all areas. gram-negative bacteria such as enterobacter associated with hospital infections were resistant to penicillins and ce ... | 1989 | 2504618 |
| [enteroinvasive escherichia coli o29:h--isolated from travellers' diarrheal cases]. | escherichia coli strains isolated in tokyo from stool cultures of two sporadic diarrheal cases of travellers returning from south america and thailand in 1985 and 1987 respectively, were found to be enteroinvasive strains with rare serotype o29:h--. these e. coli o29:h-- strains showed typical biochemical reactions of enteroinvasive e. coli in that they were negative lysine decarboxylase and non-motile. they were positive for serèny test with guinea pig eye, cell-invasion test with hela cells, 1 ... | 1989 | 2504835 |
| [clinical studies on patients with legal communicable diseases encountered from 1981 to 1986]. | patients with legal communicable diseases admitted to kawasaki municipal hospital between 1981 and 1986 were studied. ages of patients were over 15 year-old. during this period 115 patients were admitted. out of these patients 84 had shigellosis, and 9 had amebiasis. out of 20 salmonellosis, 18 were due to salmonella typhi and 2 were due to salmonella paratyphi-a, respectively. two were admitted because of cholera. out of the patients with shigellosis, 27 were domestic and 57 were foreign infect ... | 1989 | 2504839 |
| [in vitro antibacterial effect of a new oral cephalosporin, cefixime. results of a multicenter study]. | minimal inhibitory concentrations (mic) of cefixime (cxm) were evaluated by agar dilution against 2,469 bacterial strains isolated in 10 hospitals. for enterobacteriaceae, mic 50 and 90% micrograms/ml were respectively: (i) naturally non beta lactamase producing species: e. coli and shigella 0.25-0.5; salmonella 0.06-0.25; p. mirabilis 0.008-0.032. (ii) chromosomal penicillinase producing species: klebsiella 0.06-2. (iii) chromosomal cephalosporinase producing species: e. cloacae and c. freundii ... | 1989 | 2508039 |
| [the isolation and immunologic study of ribosomal preparations from shigella flexneri]. | s. flexneri ribosomal preparations were isolated by differential centrifugation or by fractionation with polyethylene glycol-6000. their chemical composition and spectrophotometric properties were characteristic of ribosomes, and, as shown by the results of the serological assay, the content of o-specific component was, on the average, 1.4%. the ribosomal preparations were nontoxic for mice when injected intraperitoneally and intravenously in large doses and induced systemic o-antibody response ... | 1989 | 2511706 |
| [increased efficiency in the disinfection of sewage by lactic acid bacteria]. | the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ... | 1989 | 2514340 |
| shigellae and vibrionaceae species as a cause of diarrhoea among children in aden. | in aden, the incidence of diarrhoea among infants and children is still high. the present work aimed at investigation of shigelleae and vibrionaceae as etiologic agents of enteric infections. stool specimens obtained from 1056 children complaining of diarrhoea were cultured on plating media and on enrichment media. suspected colonies were then serotyped and antibiotic sensitivity tests performed. seven percent of the specimens were found positive for shigella strains. shigella boydii was the com ... | 1989 | 2519964 |
| identification of shiga-like toxin in escherichia coli strains, etiological agents of diarrheal disease. | shiga-like toxin presence, in 20 e. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees c, in the presence of chloroform and by i.v. inoculation in mice. in 4 out of 20 strains, shiga-like toxin in high titres was identified. most of the strains presented an inconstant and variable production of shiga-like toxin in comparison with shigella dysenteriae type 1 (shiga) reference strain. the authors also confirm the existence of shiga-like toxin und ... | 1989 | 2520669 |
| [bacterial isolation in infants hospitalized for acute diarrhea]. | bacteria were investigated in stools of 156 children under two years of age admitted to the pediatric wards of a general hospital at the western metropolitan area of santiago, chile, because of acute diarrhea with only one stool sample. a known pathogenic agent was isolated from 115 cases (73.7%), this being a bacteria in 87/115 (75.6%). most prevalent microorganisms were enteropathogenic escherichia coli (epec). (65.5%), specially serogroups 0111, 0119 and 055, enterotoxigenic escherichia coli ... | 1989 | 2520838 |
| [antibacterial effect of cefixime]. | cefixime (cfm) is a new hemi-synthetic orally active cephalosporin which exhibits a particular affinity for pbps 3, 1a, 1bs. its penetration through the gram negative bacilli outer membrane is similar to that of third generation cephalosporins. the mics were assessed by the agar dilution method against 2,489 bacterial strains collected in 10 hospitals. against enterobacteriaceae, mics50 and 90 are respectively (mg/l): naturally non beta-lactamase-producing species: e. coli and shigella: 0.25-0.5 ... | 1989 | 2530528 |
| [antibacterial effect of cefixime in the presence of the type of beta-lactamases produced by enterobacteriaceae]. | the susceptibility of isolates or enterobacteriaceae to orally absorbed beta-lactams (amoxicillin, cephalexin, cefaclor, cefuroxime, and cefixime), was maximum for the iminomethoxy-aminothiazolyl-cephalosporin but variable according to bacterial species. for e. coli, p. mirabilis, salmonella, shigella, k. pneumoniae, k. oxytoca (group 1) mic50 were congruent to 0.06 mg/l, and mic90 congruent to 0.12 mg/l. finally for c. freundii, e. aerogenes, e. cloacae, s. marcescens, m. morganii, mic50 were h ... | 1989 | 2530532 |
| shigellosis outbreaks at summer camps for the mentally retarded in new york state. | during 1987, four new york state summer camps for the mentally retarded and developmentally disabled experienced outbreaks of shigella sonnei gastroenteritis. cases occurred in 150 of 286 (attack rate (ar) = 52%), 167 of 295 (ar = 57%), and 25 of 114 (ar = 22%) persons in three camps, respectively; a fourth camp reported eight cases. epidemiologic investigation suggested point-source foodborne outbreaks in two camps, while person-to-person spread appeared to predominate in the other two. numerou ... | 1989 | 2530896 |
| [changes in the etiologic structure of sonne shigellosis in its multiyear dynamics]. | the dynamics of sonne dysentery morbidity in connection with changes in the structure of s. sonnei circulating among the population of leningrad for the period of 1959-1984 was studied. considering such sign as the leading fermentovar, three smaller periods were established in this stretch of time. changes in the structure of circulating shigellae were accompanied by changes in the intensity and direction of tendencies or decrease in the manifest and asymptomatic forms of infection, as well as i ... | 1989 | 2531524 |
| genetics and molecular biology of siderophore-mediated iron transport in bacteria. | the possession of specialized iron transport systems may be crucial for bacteria to override the iron limitation imposed by the host or the environment. one of the most commonly found strategies evolved by microorganisms is the production of siderophores, low-molecular-weight iron chelators that have very high constants of association for their complexes with iron. thus, siderophores act as extracellular solubilizing agents for iron from minerals or organic compounds, such as transferrin and lac ... | 1989 | 2531838 |
| [cost and effectiveness of fecal culture in the etiologic diagnosis of acute diarrhea]. | to study the cost-effectiveness of fecal culture in the detection of enteropathogenic or enteroinvasive bacteria, a review and analysis was done of fecal cultures from ambulatory or hospitalized patients treated for acute diarrhea at a buenos aires sanatorium during 1985. of 1,295 fecal cultures performed, pathogens were isolated in 369 cases (28.5%), of which 79 corresponded to shigella sp. (s. flexnerii, 49; s. sonnei, 29; s. dysenteriae, 2), 25 to salmonella sp., and 270 to enteropathogenic e ... | 1989 | 2533867 |
| human calicivirus-associated diarrhea in children attending day care centers. | we investigated human calicivirus (hcv)-associated diarrhea in children attending day care centers by using stool specimens collected in 1981-1983. we used a screening enzyme-linked immunosorbent assay (elisa) derived from reagents prepared against the sapporo strain of hcv and confirmed positive results with a blocking elisa and immunosorbent electron microscopy. hcv was detected in 11 (2.9%) of 375 diarrheal stools and in none of 86 stools from asymptomatic contacts. this incidence rate was ha ... | 1989 | 2535867 |
| identification of icsa, a plasmid locus of shigella flexneri that governs bacterial intra- and intercellular spread through interaction with f-actin. | the capacity of shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin d and involvement of f-actin was demonstrated by direct labeling of infected cells with the specific dye n-(7-ni ... | 1989 | 2542950 |
| frameshifting is required for production of the transposase encoded by insertion sequence 1. | insertion sequence is1 has two coding frames, insa and insb, which are essential for its transposition. here, we show that a frameshifting event in the -1 direction from the 3' end region of the insa frame to an open reading frame (b' frame), extending from the 5' end of the insb frame, is involved in production of the insa-b'-insb fusion protein that has is1 transposase activity. the frameshifting event is likely to have occurred at the sequence aaaaac where the insa frame overlaps the b' frame ... | 1989 | 2543983 |
| [the effect of gram-negative bacteria lipopolysaccharides on the development of rauscher leukosis in balb/c mice]. | the dose-dependent action of shigella sonnei lipopolysaccharide (lps) on the development of acute erythroleukocytosis, as well as rauscher chronic myeloid and lymphoid leukosis, in balb/c mice sensitive to rauscher virus was shown. bordetella pertussis lps in the doses used in this investigation stimulated the development of both acute erythroleukosis and chronic myeloid and lymphoid leukosis in balb/c mice infected with rauscher virus. lipid a isolated from b. pertussis lps was found to produce ... | 1989 | 2544069 |
| functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in shigella flexneri 2a. | the 7 kb virulence region-2 of the large (virulence) plasmid in shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. insertion and deletion mutagenesis, dna subcloning and sds-polyacrylamide gel electrophoresis of proteins synthesized in minicells demonstrated five genes in this region. they encode 24, 18, 62 (ipab), 41 (ipac) and 37 (ipad)-kilodalton (kd) proteins. complementation of tn5-induced mutations in region-2 with the above plasmid construc ... | 1989 | 2552264 |
| enterotoxin induced diarrhoea--an update. | the pathogenic personality or the criteria required to be a successful pathogen, of enteric bacteria includes, among others, the ability to produce potent proteins which by different intracellular mechanisms elicit what we overtly see as diarrhoea. enteropathogens belonging to several genera like vibrio, escherichia, shigella, salmonella, campylobacter, aeromonas and yersinia include species capable of elaborating strikingly similar exotoxins which seem to share common mechanisms of action invol ... | 1989 | 2554547 |
| [causes of infectious gastro-enteritis in children in dakar]. | between february 1983 and may 1988, 1,157 stools from children aged under 15 years presenting with diarrhoea and admitted to a paediatric hospital in dakar, senegal, were examined for the presence of bacterial and parasitic agents. we looked for campylobacter and rotavirus in only 245 and 111 samples respectively. enterobacteria were detected most frequently (162/264; 61.3 per cent). among these, we found 92 (34.8 per cent) strains of enteropathogenic e. coli; 40 strains (15.1 per cent) of salmo ... | 1989 | 2555813 |
| phosphate regulon in members of the family enterobacteriaceae: comparison of the phob-phor operons of escherichia coli, shigella dysenteriae, and klebsiella pneumoniae. | the structure and function of the phob and phor genes of shigella dysenteriae strains and klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. complementation tests among the genes of escherichia coli, s. dysenteriae strains, and k. pneumoniae for production of alkaline phosphatase indicate that s. dysenteriae serotype 2 and serotype 3 strains and k. pneumoniae are phoa+ phob+ phor+ but s. dysenteriae sh and serotype 1 strains are phoa phob+ phor. nucl ... | 1989 | 2556368 |
| comparative in vitro and in vivo activities of piperacillin combined with the beta-lactamase inhibitors tazobactam, clavulanic acid, and sulbactam. | tazobactam (ytr-830h), a novel beta-lactamase inhibitor, was compared with clavulanic acid and sulbactam for enhancement of the activity of piperacillin against beta-lactamase-producing, piperacillin-resistant clinical isolates. piperacillin mics were determined in media containing a fixed concentration of 2 or 4 micrograms of the inhibitors per ml. the higher concentration was generally more effective. tazobactam was superior to sulbactam in enhancing the spectrum and potency of piperacillin. a ... | 1989 | 2558615 |
| epidemiology and aetiology of acute childhood diarrhoea in burma: a rural community survey. | identical epidemiological and microbiological surveys were carried out in a rural community at intakaw, burma, in 2 months, one in the hot wet and the other in the cool dry season. the incidence of diarrhoea was highest in children under 3 years old and in the hot wet month when enterotoxigenic escherichia coli was the predominant pathogen. rotavirus was the commonest pathogen in the cool dry month but was absent in the hot wet month. shigellae, salmonellae and campylobacter were isolated in bot ... | 1989 | 2559512 |
| studies to optimize the in vitro testing of piperacillin combined with tazobactam (ytr 830). | the combination of piperacillin and the beta-lactamase inhibitor tazobactam (formerly ytr 830) was studied to determine optimal disk concentrations and dilution testing conditions. in addition, the potency of the combination was compared to that of piperacillin alone. the spectrum of piperacillin was greatly expanded by the addition to tazobactam principally against beta-lactamase producing strains of haemophilus influenzae, escherichia coli, morganella morganii, proteus vulgaris, providencia st ... | 1989 | 2560422 |
| infection and cross-infection in a paediatric gastro-enteritis unit. | a two month study to investigate the incidence of nosocomial infection was conducted in a paediatric gastroenteritis ward of a black academic hospital. enteric pathogens were identified on admission in 61 (47.2%) of 129 patients; 56 bacterial and 25 viral. six per cent of patients had a combination of bacterial and viral pathogens. enteric pathogens most frequently identified on admission were campylobacter jejuni in 22%. rotavirus in 19.3%, epec in 10.8% and shigella spp. in 6.9% patients. twen ... | 1989 | 2561089 |
| detection of enteropathogens in diarrhoeal diseases among malnourished egyptian infant and children. | the influence of the pre-existing malnutrition (pem) on the severity of diarrhoea as well as the causative organisms was studied on 60 cases. the duration of diarrhoea was prolonged in cases with pem. the stool purgative rate ranged from 4-15 times/day in pem infant while it was 3-6 times in well nourished cases (wnc) (p less than 0.05). also vomiting and dehydration was more marked among pem cases (52.9% and 32.4% of cases than in wnc cases (31.3% and 12.5% of cases) (p less than 0.05). rota vi ... | 1989 | 2562440 |
| examination of colonies and stool blots for detection of enteropathogens by dna hybridization with eight dna probes. | we compared three methods for detecting enteropathogens in 416 children with diarrhea: (i) examination of 10 lactose-fermenting and all non-lactose-fermenting escherichia coli (colony blots); (ii) examination of 300 colonies (replicate blots); and (iii) determination of the total bacterial growth of stools (stool blots). all specimens were spotted onto whatman 541 filters and hybridized with specific radiolabeled dna probes. enterotoxigenic e. coli was detected in 38 patients by examining colony ... | 1989 | 2563385 |
| endocytosis from coated pits of shiga toxin: a glycolipid-binding protein from shigella dysenteriae 1. | evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from shigella dysenteriae 1, shiga toxin, which acts by removal of a single adenine residue in 28-s ribosomal rna. inhibition of endocytosis by atp depletion of the cells prevented toxin uptake. exposure of hela s3 and vero cells to toxin at low extracellular ph, where translocation to the cytosol, but not endocytosis is inhibited, allowed the toxin to accumulate in a compartment where it was prot ... | 1989 | 2564398 |
| common themes in microbial pathogenicity. | a bacterial pathogen is a highly adapted microorganism which has the capacity to cause disease. the mechanisms used by pathogenic bacteria to cause infection and disease usually include an interactive group of virulence determinants, sometimes coregulated, which are suited for the interaction of a particular microorganism with a specific host. because pathogens must overcome similar host barriers, common themes in microbial pathogenesis have evolved. however, these mechanisms are diverse between ... | 1989 | 2569162 |
| shigella dysenteriae type 1 infections in us travellers to mexico, 1988. | in 1988, the number of shigella dysenteriae type 1 (sd1) infections reported in the usa increased five-fold over the annual mean from the previous decade. 44 (94%) of 47 interviewed patients reported recent travel to mexico; 33 (75%) of these had been tourists to the yucatan peninsula. 27 patients who had travelled to mexico were admitted to hospital, of whom 2 had a haemolytic uraemic syndrome; none died. the antimicrobial resistance pattern and plasmid profile of the yucatan strain were simila ... | 1989 | 2570242 |
| nosocomial gastroenteritis in paediatric patients. | between november 1982 and april 1985, 2228 children under the age of 5 years with acute gastroenteritis were admitted to the paediatric isolation ward of queen mary hospital, department of paediatrics, university of hong kong. in 56.2% the causative agent was identified as rotavirus, salmonella, campylobacter, shigella, escherichia coli or a combination of these pathogens. our isolation procedures included cohort nursing of all diarrhoeal patients in two separate rooms (each accommodating 6 pati ... | 1989 | 2575634 |
| [rheumatologic syndrome in gastrointestinal diseases. clinical and pathogenetic aspects]. | patients with reactive arthritis, sacroiliitis, spondylitis or reiter's syndrome following intestinal infection from yersinia, salmonella, shigella or campylobacter organisms have been reported from endemic areas and after epidemic dysenteries. possession of the antigen hla b27 affects severity and prognosis of the arthritis and is more often associated with spondylitis and reiter's syndrome. articular manifestations are also quite common systemic complications of chronic inflammatory bowel dise ... | 1988 | 2577931 |
| [synthesis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides. ii. synthesis of trisaccharide glc alpha1----3rha alpha1----2rha alpha1----ome and tetrasaccharide glcnac beta1----2(llc alpha1----3)rha alpha1----2rh alpha1----ome]. | methyl glycosides of the title linear trisaccharide and branched tetrasaccharide were synthesized by stepwise glycosylation. these oligosaccharides represent the fragments of o-antigenic polysaccharides of shigella flexneri serotypes 2b, 3a, 5b, and x. | 1985 | 2581587 |
| inflammatory bowel disease. | the place of inflammatory bowel disorders in the spondarthritis matrix has been discussed and aetiopathological and clinicoradiological features of their individual arthropathies described. particular emphasis has been placed on the arthropathies of ulcerative colitis and crohn's disease, but a comment is also included on the much rarer condition, whipple's disease. the position of reactive arthritis resulting from enteric infection in relation to the spondarthritis concept is examined in the li ... | 1985 | 2581732 |
| [effect of specimen workup on detection of shigella antigens]. | 1985 | 2582197 | |
| the role of shigella spp. in childhood diarrhoea in iran and their antibiotic resistance. | the incidence of shigellae was measured in 445 children with diarrhoea in sanandaj, kordestan, and 715 in tehran during a 1-year study. 18 (4%) patients in sanandaj and 41 (5.7%) patients in tehran were positive for various shigella species. sh. boydii (7 strains) and sh. sonnei (25 strains) were the commonest species in sanandaj and tehran respectively. among the isolates from sanandaj the highest resistance was to tetracycline (55.5%), whereas in tehran it was to sulfamethoxazole/trimethoprim ... | 1989 | 2587943 |
| comparative in vitro activity and beta-lactamase stability of fk482, a new oral cephalosporin. | fk482 is an oral aminothiazolyl hydroxyimino cephalosporin with a c-3 vinyl group. its activity was compared with those of cephalexin, cefuroxime, cefixime, and amoxicillin-clavulanate. fk482 inhibited 90% of staphylococcus aureus isolates at 1 micrograms/ml and 90% of streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae isolates at less than or equal to 0.012 micrograms/ml, superior to cephalexin and cefuroxime and similar to cefixime. it did not inhibit oxacillin-resi ... | 1989 | 2589845 |
| high incidence of false positives by a latex agglutination test for the diagnosis of clostridium difficile associated colitis in compromised patients. | detection of clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitis has been used for over a decade. because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (la) kit has attracted much attention. we compared the sensitivity and specificity of this method with the cytotoxic assay (cta) using diarrheal stools from 652 patients at a referral tertiary care center. specimens from 71 (10.9%) pa ... | 1989 | 2591165 |
| [enteropathogens detected in healthy children in 3 low income communities, in recife, state of pernambuco, brazil]. | stools of 646 healthy children between zero and five years of age who live in 3 communities of slightly different economic levels and sanitary conditions were investigated for enteropathogenic escherichia coli (epec), enteroinvasive e. coli (eiec), shigella and salmonella. cultures were positive for enterophatogens in 82 (12.69%) of the children. epec was the most frequent isolate (6.04%) followed by shigella (4.18%) and salmonella (2.17%). invasive e. coli (eiec) was detected only twice. accord ... | 1988 | 2593819 |
| causes of death and the histopathologic findings in fatal shigellosis. | thirty-seven children (median age, 2 years) with shigellosis in bangladesh were subjected to postmortem examination to determine causes of death and the spectrum of intestinal histopathology. infecting species were: shigella dysenteriae 1, 7 cases; s. dysenteriae 2, 2 cases; shigella flexneri, 23 cases; shigella boydii, 4 cases; and mixed infection with shigella boydii and shigella sonnei, 1 case. complicating conditions detected before death included malnutrition in 25 cases, pneumonia in 11 ca ... | 1989 | 2594452 |
| exotic diarrhoeal problems and poliomyelitis. | exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death. | 1989 | 2594659 |
| [restriction-modification systems of type ii in shigella strains]. | two restriction-modification systems specified by two plasmids are discovered in the clinical species of shigella. the plasmids are designated pkmr114 and pkmr115. both are of 60.800 bp and belong to the incn incompatibility group. the ecori, ecorv, hindiii restriction patterns of both plasmid dnas are identical. as shown by efficiency of plating of bacteriophage lambda vir on the strains harbouring plasmids encoding ecori, ecorii, ecoriii, ecoriv, ecorv systems and plasmids studied, the discove ... | 1989 | 2599373 |
| serological investigations of children in iran for campylobacter, yersinia and shigella. | serological investigations for campylobacter, yersinia and shigella in hospitalized children (different hospitals) in iran revealed a contamination rate of 15% for campylobacter, up to 19% for yersinia and between 1 and 12% for the various strains of shigellae. in diarrhoeal diseases the possibility of an infection with campylobacter, yersinia and shigellae must be considered. | 1989 | 2599389 |
| study of rwandese medicinal plants used in the treatment of diarrhoea i. | a series of 20 medicinal plants used in the rwandese traditional medicine to treat diarrhoeal diseases has been screened for antibacterial activity against several salmonella and shigella and for antidiarrhoeal activity on mice. fifty percent of the plants tested showed antibacterial activity against one or more microorganisms and 65% showed antidiarrhoeal activity. | 1989 | 2601351 |
| [infectious diarrhea in the adult]. | infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ... | 1989 | 2602888 |
| bacterial pathogens isolated from cockroaches trapped from paediatric wards in peninsular malaysia. | a survey was conducted in 4 paediatric wards in malaysia to determine the distribution of various species of cockroaches and to examine their gut contents for bacteria. cockroaches were trapped from food dispensing areas (kitchens), store rooms, cupboards and open wards. 104 cockroaches were caught, consisting of periplaneta americana (67.3%), blattella germanica (26%), p. brunnea (4.8%), and supella longipalpa (1.9%). bacteria were isolated from all cockroaches except 3 p. americana. many bacte ... | 1989 | 2603192 |
| mixed infections in childhood diarrhoea: results of a community study in kiambu district, kenya. | one thousand four hundred and twenty diarrhoea specimens from 846 children aged 0 to 60 months were collected and analysed for bacteria, parasites and rotavirus over a 16 month period, from june 1985 to september 1986 inclusive. the study was conducted in 4 villages situated in kiambu district, kenya. all the specimens were analysed for rotavirus and parasites, including cryptosporidium. the majority of the specimens were analysed for enteropathogenic escherichia coli (epec), shigella, salmonell ... | 1989 | 2606013 |
| signal structure of the cis-acting element recognized by virg protein, a positive regulator in agrobacterium. | virg protein is known to be the positive regulator for vir genes of ri and ti plasmids in agrobacterium. to investigate the cis-acting element recognized by virg, we have determined the transcriptional start points of the vir genes on ri plasmid (pria4b). from the analysis of the upstream sequences, it has been found that the sequences generally similar to 5'tg(a/t)aa(c/t)3' appear in phase with an 11-base pair interval, and that the -35 and -10 regions of the promoters are located nearly in the ... | 1989 | 2608465 |
| symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984. | the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ... | 1989 | 2609210 |
| cockroaches (periplaneta americana) as carriers of agents of bacterial diarrhoea in accra, ghana. | bodies and intestinal contents of 208 cockroaches (periplaneta americana), collected from kitchens in accra and some surrounding villages were cultured for enteric bacterial pathogens. six of them harboured three different serogroups of salmonella, one had shigella dysenteriae, 64 had coliforms, 13 had proteus species, two had pseudomonas species and the rest (122) carried none of the bacterial species mentioned above. the presence of salmonella species shigella dysenteriae and coliforms in thes ... | 1989 | 2611875 |
| in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea. | the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ... | 1989 | 2613604 |
| antimicrobial susceptibility of selected bacterial enteropathogens in latin america and worldwide. | we conducted an in vitro susceptibility study of bacterial pathogens to various antimicrobials. strains of shigella, salmonella, escherichia coli, and klebsiella pneumoniae collected in the 1960s, 1970s, and 1980s at the hospital infantil de mexico federico gomez were tested against ampicillin, chloramphenicol, tetracycline, trimethoprim-sulfamethoxazole, amikacin, gentamicin, and furazolidone. over the 3-decade period, the resistance of enteropathogens to furazolidone showed the least overall i ... | 1989 | 2617180 |
| in vitro susceptibility of shigella strains isolated from stool cultures of dysenteric patients. | a reprospective analysis was conducted on the susceptibility by diffusion of 100 shigella strains to 8 antimicrobial agents. these strains were isolated from 852 stool cultures obtained from dysenteric patients seen between january 1986 and april 1987. seventy-one per cent of the samples from which shigella sp were isolated were obtained from children under age 6 years; 86% of these samples revealed pus, with or without blood. the shigella serotypes isolated most often were s. flexneri (57%) and ... | 1989 | 2617181 |
| depletion of hepatic glycogen in the hypoglycaemia of fatal childhood diarrhoeal illnesses. | to determine whether depletion of liver glycogen or accumulation of liver fat (steatosis) was associated with the development of hypoglycaemia in children with fatal diarrhoeal illnesses, a case-control study was carried out comparing 17 children who had blood sugars less than or equal to 30 mg/dl with 17 age matched control children who had blood sugars greater than or equal to 59 mg/dl. the most common causes of diarrhoea in the hypoglycaemic children were shigella sp. and vibrio cholerae. the ... | 1989 | 2617656 |
| [long-term cryopreservation of microbial strains relevant to food hygiene]. | experiments on long-time cryopreservation at -20 degrees c were conducted, using 42 microbial strains of relevance to food hygiene. blood slant agar was coated with 10% glycerol solution, so that all strains were in re-culturing conditions, following 12 months of storage. re-culturing of 18 in 20 of the microbial genera used in the experiment was possible after 24 months of storage, if strains had been kept only in 10% glycerol solution. the method proved suitable particularly for strain keeping ... | 1989 | 2619482 |
| gas liquid chromatography of amines produced by the enterobacteriaceae. | 1. gas liquid chromatography (glc) was used to test the production of amines by 85 strains of enterobacteriaceae. 2. the strains tested produced cadaverine, beta-phenylethylamine, putrescine, iso-amylamine, 2-methylbutylamine and iso-butylamine. 3. although the overlap in amine production between obviously different genera and species limits the general applicability of this methodology in clinical microbiology, the results obtained demonstrate the possibility of differentiating proteus rettgeri ... | 1989 | 2620174 |
| persistent diarrhoea in a rural area of bangladesh: a community-based longitudinal study. | as part of a health impact evaluation of a water supply and sanitation project in a rural area of bangladesh, diarrhoeal morbidity was recorded in children 0-4 years of age using weekly recall in household interviews, during the period march 1984 to december 1987. during the baseline year, 1984, the incidence rate of all diarrhoea episodes (3.8 episodes per child per year), and those defined as persistent, duration greater than 14 days (0.6 episodes per child per year), showed a similar age dist ... | 1989 | 2621034 |
| increased efficiency of stool culture for the detection of salmonella and shigella. | the wampole bactigen salmonella-shigella latex agglutination test (ssla) (wampole laboratories, cranbury, new jersey) was evaluated as a possible substitute for blind subculture of selenite broths from stool cultures. recovery rates of salmonella and shigella from eosin-methylene blue (emb) agar were reviewed to determine if this medium could be eliminated from primary stool culture. salmonella was detected in 17 of 822 stools by both ssla and culture. there were 52 false-positive ssla for salmo ... | 1989 | 2625035 |
| evaluation of coagglutination test for serotyping of enteropathogenic bacteria. | results of conventional agglutination and coagglutination (coa) tests for serotyping of enteropathogenic escherichia coli (epec), shigella, salmonella and vibrio cholerae were compared. eighty isolates of different serotypes of epec falling in the wellcome polyvalent (ok) 2, 3 and 4 antisera, showed good (4+) agglutination with coa reagent up to 1:960 dilution. out of 50 strains of shigella species tested, 25 of sh. dysenteriae and 5 of sh. sonnei gave good reaction up to 1:1920 dilution and 15 ... | 1989 | 2628290 |
| efficacy of nalidixic acid in the treatment of acute bloody diarrhea. | of sixty four children (mean age 20.1 +/- 1.2 mo) with acute bloody diarrhea and high fever, 47 had infection with non-typhoidal salmonella (nts) (20), shigella (15) and enteropathogenic e. coli (epec) (12) and were treated with nalidixic acid (na). the mean duration (h) of presence of macroscopic blood in the stool following institution of treatment was significantly shorter (p less than 0.05) in those with epec (11.5 +/- 4.9) as compared to nts (30.4 +/- 15.4) or shigella groups (22.9 +/- 15.6 ... | 1989 | 2630475 |
| carriage of enteric bacterial pathogens by house flies in yangon, myanmar. | the carriage of enteric bacterial pathogens by house flies, caught from different places of human habitation and at different seasons, was quantitatively determined in the town of tamwe, yangon, myanmar. a total of 186 fly-pools, each containing 10 live flies, were caught in entomological cages by random sampling regarding season, month, frequency and place of collection. a 10% suspension of each homogenised fly-pool in sterile normal saline was prepared on the day of collection and was cultured ... | 1989 | 2632639 |
| bacterial and viral causes of acute diarrhoea in children in kuwait. | bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ... | 1989 | 2632640 |
| [frequency and characteristics of campylobacter jejuni-coli diarrhea in tunisia]. | because importance of campylobacter jejuni/coli in human disease, we studied frequency, clinical and biological symptoms of campylobacter enteritis. investigation has been realised on 216 cases of diarrhoea seen outside the hospital and aged from 4 days to 60 years, and 512 cases of hospitalised diarrhoea. outside the hospital, this bacteria has the same frequency as shigella and salmonella (3-5%). inside the hospital, it is rare. outside the hospital, this bacteria is isolated in babies and chi ... | 1989 | 2633873 |
| [pathogenic agents in acute non-enterocolic diarrheal syndrome]. | along a one year period 112 infants admitted with non enterocolic acute diarrhea were studied for isolation of potentially ethiologic agents, namely enteropathogenic bacteria (salmonella, shigella, campylobacter, classic enteropathogenic, enteroinvasive and enterotoxigenic escherichia coli), rotavirus (viral rna electrophoresis) and enteroparasites (telemann and pafs). the most frequently identified pathogen was rotavirus (57.8%), followed by thermo labile toxin producing escherichia coli (19.7% ... | 1989 | 2634863 |
| a clinical evaluation of sulbactam/ampicillin in the treatment of pediatric infections. | we have treated 42 episodes of pediatric infections with sulbactam/ampicillin since 1987. included were 9 cellulitis, 9 urinary tract infections, 5 cervical lymphadenitis, 4 meningitis, 2 thoracic empyema, 2 osteomyelitis, 2 sepsis, 1 furuncle, 1 perianal abscess, 1 dental abscess, 1 peritonsillitis, 1 salmonellosis, 1 shigellosis, 1 peritonitis, 1 suppurative thyroiditis, 1 infective endocarditis. responsible pathogens were escherichia coli in 8, staphylococcus aureus in 6, hemophilus influenza ... | 1989 | 2637593 |