Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| increased colonization potential of campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. | the mechanisms by which campylobacter jejuni rapidly spreads through large broiler flocks are unknown. recent evidence suggests that the minimum infective oral dose for 100% caecal colonization is about 10(4) cfu, which, with such a 'fragile' organism, may limit transmissibility. however, the colonization potential of laboratory-adapted strains may be anomalous compared with fresh isolates or those passaged in vivo. the colonization potential of two chicken and one human c. jejuni isolates, whic ... | 1996 | 8760971 |
| campylobacter jejuni infections and anti-gm1 antibodies in guillain-barré syndrome. | the group of patients with guillain-barr'e syndrome (gbs) is very heterogenous with regard to antecedent infections, immunological parameters, clinical manifestations, and response to treatment. in this study, the presumed pathogenic factors anti-gm1 antibodies and campylobacter jejuni infections were related to the clinical characteristics. serum from 154 patients with gbs, 63 patients with other neurological diseases (ond), and 50 normal controls (nc) were tested for the presence of antibodies ... | 1996 | 8773599 |
| guillain-barré syndrome in children. | the guillain-barré syndrome is a pediatric neurologic emergency and the most common cause in children of rapidly evolving, usually flaccid, weakness with associated areflexia. two treatable illnesses, namely tick paralysis and acute cord compression, demand immediate differential diagnosis. rarely, poliomyelitis still mimics infantile guillain-barré syndrome. specific precursor infections, such as campylobacter jejuni, are now recognized to affect the clinical presentation of guillain-barré synd ... | 1995 | 8776016 |
| experimental hepatitis induced by campylobacter jejuni infection in japanese quail (coturnix coturnix japonica). | to establish an experimental model for vibrionic hepatitis caused by campylobacter jejuni, japanese quails (coturnix coturnix japonica) were inoculated with c. jejuni strains isolated from chicken hepatitis (bl107) and human diarrhea (hp5113). necrotic liver lesions were formed by intra-pancreaticoduodenal vein injection by which the bacteria reached the liver directly via the portal vein, but not by intra-gastric infection. these liver lesions were observed from day 1 to 7 after the infection. ... | 1996 | 8777226 |
| infection with ciprofloxacin-resistant campylobacter jejuni in travellers returning from asia. | 1996 | 8781891 | |
| antimicrobial activity of rifabutin. | rifabutin is a spiro-piperidyl-rifamycin derived from rifamycin-s. it is structurally related to rifampin and shares many of its properties. rifabutin has a broad spectrum of antimicrobial activity. it is considerably more active than rifampin in vitro against the mycobacterium avium complex (mac), mycobacterium tuberculosis, and mycobacterium leprae. it also is active against most atypical mycobacteria, including mycobacterium kansasii, but mycobacterium chelonae is relatively resistant. rifabu ... | 1996 | 8785253 |
| comprehensive ribotyping scheme for heat-stable serotypes of campylobacter jejuni. | strains from diverse sources belonging to all 47 heat-stable penner serotypes of campylobacter jejuni were examined for polymorphism around the 16s rrna genes. penner serotype reference strains and a group of nonserotypeable isolates were included in the study. complete typeability was obtained; 30 distinct psti and 42 haeiii polymorphisms were found. three bands were detected in almost all strains with these enzymes, confirming that three copies of the 16s rrna gene are typical for c.jejuni. by ... | 1996 | 8788998 |
| analysis of hl and o serotypes of campylobacter strains by the flagellin gene typing system. | we recently developed a molecular typing system for campylobacter jejuni and campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaa (i.nachamkin, k. bohachick, and c.m. patton, j. clin. microbiol. 31:1531-1536, 1993). we extended the typing system to 83 flagellin types (designated flaa-1,flaa-2, etc.) on the basis of analysis of 404 isolates of c. jejuni and c. coli including common serotypes isolated in the united states, a selection of less com ... | 1996 | 8789000 |
| an environmentally regulated pilus-like appendage involved in campylobacter pathogenesis. | examination of strains of campylobacter jejuni, campylobacter coli, and campylobacter fetus by electron microscopy revealed that they produced peritrichous pilus-like appendages when the bacteria were grown in the presence of bile salts. various bile-salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype. morphologically, the pili were between 4 and 7 nm in width a ... | 1996 | 8793884 |
| experimental campylobacter jejuni infection in the chicken: an animal model of axonal guillain-barré syndrome. | to develop and characterise an animal model of paralytic neuropathy after campylobacter jejuni infection. campylobacter infection precedes development of many cases of guillain-barré syndrome and is particularly associated with cases having prominent axonal degeneration. understanding the pathogenesis of guillain-barré syndrome after c jejuni infection has been slowed by the lack of animal models. | 1996 | 8795599 |
| cytomegalovirus infection and guillain-barré syndrome: the clinical, electrophysiologic, and prognostic features. dutch guillain-barré study group. | guillain-barré syndrome (gbs) is usually preceded by infections, in particular cytomegalovirus (cmv) and campylobacter jejuni infection. we studied the clinical and electrophysiologic features of 20 cmv-associated gbs patients and compared the findings with earlier established data of c. jejuni-related gbs patients (n = 43) and of gbs patients without these infections (n = 71). the patients all participated in the dutch gbs trial in which we compared the effect of intravenous immune globulins an ... | 1996 | 8797462 |
| transcription of the campylobacter jejuni cell division gene ftsa. | as part of a study of genes whose transcription is maintained in stationary phase, cloned segments of dna were selected from a lambda zap ii library of campylobacter jejuni nctc 11,168. one such clone was found to encode a homologue of the escherichia coli cell division gene ftsa. examination of mrna by transcription mapping revealed that the campylobacter gene has one major and three minor transcription start sites. there were several significant differences in the structure and organisation of ... | 1996 | 8807806 |
| [the e test and quinolone-resistant campylobacter jejuni]. | the e-test is a quantitative technique for the determination of antimicrobial sensitivity. the system is based on the diffusion of a predefined exponential gradient in a determined antibiotic, using an inert plastic support which is applied to a solid culture medium and it is an alternative method for antibiotic sensitivity testing. of the series published, some specifically include microorganisms considered fastidious or difficult to grow, such as campylobacter jejuni. in recent years a progres ... | 1995 | 8808474 |
| construction of a ferritin-deficient mutant of campylobacter jejuni: contribution of ferritin to iron storage and protection against oxidative stress. | the ferritin-encoding gene (cft) of campylobacter jejuni was cloned and sequenced. the nucleotide sequence of cft had a 501 bp open reading frame for a protein with 167 amino acids and a predicted molecular mass of 19 180 da, and showed a high similarity to that of helicobacter pylori and escherichia coli ferritin genes. to determine the biological function of ferritin in c. jejuni, a ferritin-deficient mutant was constructed. the growth of ferritin-deficient strain sna 1 was clearly inhibited u ... | 1996 | 8809765 |
| pcr-based restriction fragment length polymorphism (rflp) analysis and serotyping of campylobacter jejuni isolates from diarrheic patients in china and japan. | a molecular typing approach for campylobacter jejuni was applied with restriction fragment length polymorphism (rflp) analysis of a 702-bp pcr-amplified portion of the flagellin-a (flaa) gene. we analyzed a total of 179 strains, including 69 independent clinical isolates from diarrheic patients in japan, 85 isolates in china, and 25 heat-stable (hs) serotype strains by penner and hennessy (1980) j. clin. microbiol. 12, 732-737). six afai, seven mboi, and five haeiii rflps were found in the 702-b ... | 1996 | 8810495 |
| expanded genomic map of campylobacter jejuni ua580 and localization of 23s ribosomal rrna genes by i-ceui restriction endonuclease digestion. | the genomic map of campylobacter jejuni ua580 was expanded and more precisely constructed using i-ceui, sal/i and smai restriction endonucleases in conjunction with pulsed-field gel electrophoresis (pfge). the presence of three fragments after digestion with i-ceui confirmed the presence of three copies of the 23s ribosomal rrna (rrl) gene. the genome size of campylobacter jejuni ua580 was determined to be 1725 +/- 5.9 kbp by i-ceui with fragment sizes of 1053 +/- 4.4, 361 +/- 2.7 and 311 +/- 3. ... | 1996 | 8810506 |
| dna fingerprinting and serotyping of campylobacter jejuni isolates from epidemic outbreaks. | the aim of the present investigation was to compare dna fingerprinting and serotyping (heat-stabile and heat-labile antigens) of isolates from epidemic outbreaks as well as of solitary isolates. campylobacter jejuni isolates from two epidemic outbreaks in sweden, one milkborne (35 isolates) and one waterborne (17 isolates), and one waterborne outbreak in norway (11 isolates), as well as 30 solitary isolates from swedish patients with gastroenteritis, were analyzed. a total of 93 isolates were an ... | 1996 | 8815103 |
| a rapid, colourimetric assay for cytotoxin activity in campylobacter jejuni. | cell extracts and culture supernates of campylobacter jejuni nctc 11168 and three isolates from faecal samples from patients with enteritis were tested for cytotoxic activity on hela and vero cells using a sensitive and rapid dye reduction assay which represents a simple assay for cytotoxin activity that can be assessed visually or spectrophotometrically in the wells of microplates. the assay was as sensitive as trypan blue exclusion and did not require the use of radioisotopes. a low level of c ... | 1996 | 8821400 |
| fatal septic shock with multiple organ failure due to campylobacter jejuni. | 1996 | 8824999 | |
| antineutrophil cytoplasmic antibodies in reactive arthritis. | to study the occurrence of antineutrophil cytoplasmic antibodies (anca) in reactive arthritis (rea). | 1995 | 8835566 |
| a study of nosocomial diarrhea in calcutta. | nosocomial infection is a major problem in hospitalized patients, particularly those who are debilitated. these infections may manifest as diarrhea. the spectrum of infections agents causing nosocomial diarrhea in our country is not known. | 1996 | 8840619 |
| detection of campylobacter jejuni/c.coli infection in patients with guillain-barré syndrome by serology and culture. | serum samples from 43 cases of guillain-barré syndrome (gbs), 32 non-gbs neurology patients, and 35 healthy persons from a medical institute in kerala, india, were tested for antibody levels against a campylobacter jejuni strain penner serotype 0:19 by agglutination and elisa. twenty-six percent of samples from gbs cases showed high antibody levels in all assays. of 8 stool samples of new gbs cases examined by culture, 38% were positive for c. jejuni/c. col1. the results suggest that at least a ... | 1996 | 8841044 |
| antimicrobial susceptibilities of campylobacter jejuni and campylobacter coli to 12 beta-lactam agents and combinations with beta-lactamase inhibitors. | the in vitro activities of 12 beta-lactam agents against 100 thermophilic campylobacter strains were tested. beta-lactamase production was detected in 88% of all strains tested. clavulanic acid was the best inhibitor by susceptibility testing. the beta-lactams which displayed high levels of in vitro activity against campylobacter isolates were imipenem, amoxicillin-clavulanic acid, and cefepime and, to a lesser degree, amoxicillin, ampicillin, and cefotaxime. | 1996 | 8843305 |
| early nodal changes in the acute motor axonal neuropathy pattern of the guillain-barré syndrome. | the axonal patterns of guillain-barré syndrome, associated in many cases with antecedent campylobacter jejuni infection, are now recognized as frequent causes of acute flaccid paralysis in some regions of the world. this study examined ultrastructurally the pns of seven cases of the acute motor axonal neuropathy form of guillain-barré syndrome. in this disorder previous studies of advanced cases have found wallerian-like degeneration of motor fibres in the spinal roots and peripheral nerves, wit ... | 1996 | 8852937 |
| assessment of the dose-response relationship of campylobacter jejuni. | mathematical relations describing the risk of infection after exposure to enteropathogens are important tools for the evaluation of the potential health risk from exposure via food and water. a quantitative description of the dose-response relation for campylobacter jejuni with the beta-poisson model was fitted to experimental data of infection with campylobacter jejuni (as determined by shedding of c. jejuni) obtained in human feeding studies performed by black et al. (1988). the maximum likeli ... | 1996 | 8856377 |
| sequential development of resistance to fluoroquinolones and erythromycin in an isolate of campylobacter jejuni. | a severe episode of campylobacter jejuni gastroenteritis in a patient with hiv infection was treated with ciprofloxacin and, because of therapeutic failure, subsequently with roxithromycin. after treatment, c. jejuni was again isolated from feces and shown to be resistant to both drugs. we present molecular evidence of the sequential development of both types of resistance in the patient isolate. to our knowledge, this is the first case with documented evidence showing sequential emergence of re ... | 1996 | 8861869 |
| pcr-rflp analysis of the large subunit (23s) ribosomal rna genes of campylobacter jejuni. | forty-seven strains of campylobacter jejuni were examined by pcr-rflp analysis of 23s rrna genes. seven different molecular profiles were detected by a combination of hpaii alui and ddei digest analysis. most (83%) strains, including those with different penner serotypes and from different hosts, had the same molecular profiles. the high level of conservation apparent within the 23s rdna sequences confirmed their value as targets in species-specific pcr identification assays but not for subtypic ... | 1996 | 8862021 |
| sub-typing of animal and human campylobacter spp. using rapd. | based on a 10-mer primer (5'-cctgttagcc-3'), a random amplified polymorphic dna (rapd) method for typing campylobacter coli isolated from pigs was developed. the method proved effective with a high discrimination and good reproducibility. in contrast with serotyping no untypable strains were found out of a total of 269 isolates (veterinary, food and clinical) examined. the method was also successfully applied to typing campylobacter jejuni from a similar range of sources. | 1996 | 8862022 |
| autoantibodies to peripheral nerve glycosphingolipids spg, slpg, and sgpg in guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy. | unlike cns myelin, human peripheral nerve myelin has the acidic glycosphingolipids sialosyl paragloboside (spg), sialosyl lactosaminyl paragloboside (slpg), and sulfated glucuronyl paragloboside (sgpg). to elucidate the pathogenesis of guillain-barré syndrome (gbs) and chronic inflammatory demyelinating neuropathy (cidp), we investigated the autoantibodies to peripheral nerve molecules in patients with these diseases and compared the frequency of the autoantibodies with that of autoantibody to g ... | 1996 | 8862128 |
| campylobacter bacteremia in children. | to describe clinical and laboratory data for, and to propose pathogenesis and management of, children from impoverished communities with campylobacter bacteremia. | 1996 | 8866805 |
| the application of genotyping techniques to the epidemiological analysis of campylobacter jejuni. | campylobacter jejuni serogroup reference strains and collections of sporadic and outbreak-associated isolates were examined for restriction fragment length polymorphisms (rflps), using c. jejuni random chromosomal and 16s rrna gene probes. a collection of 48 penner (hs) and 14 lior (hl) serogroup reference strains, plus 10 clinical isolates, generated 35 rflp and 26 ribotype patterns. in combination the two loci generated 48 distinct genotypes. both probes were able to differentiate between cert ... | 1996 | 8870620 |
| repetitive and arbitrary primer dna sequences in pcr-mediated fingerprinting of outbreak and sporadic isolates of campylobacter jejuni. | pcr-mediated fingerprinting with combined repetitive and arbitrary dna primers (eric-2 and 1026) was used to type campylobacter jejuni from a milk-associated outbreak, and from sporadic cases of the same and allied hs serotypes. the 14 outbreak strains had identical or similar dna band profiles whereas the 25 strains from sporadic infections were more heterogeneous with 18 different dna profiles. although pcr-based dna fingerprints lacked serotype specificity, the method was fast, simple to perf ... | 1996 | 8871111 |
| acute motor axonal neuropathy: an antibody-mediated attack on axolemma. | the acute motor axonal neuropathy (aman) form of the guillain-barre syndrome is a paralytic disorder of abrupt onset characterized pathologically by motor nerve fiber degeneration of variable severity and by sparing of sensory fibers. there is little demyelination or lymphocytic inflammation. most cases have antecedent infection with campylobacter jejuni and many have antibodies directed toward gm1 ganglioside-like epitopes, but the mechanism of nerve-fiber injury has not been defined. in 7 fata ... | 1996 | 8871584 |
| an unusual case of refractory campylobacter jejuni infection in a patient with x-linked agammaglobulinemia: successful combined therapy with maternal plasma and ciprofloxacin. | an unusual hippurate-negative strain of campylobacter jejuni caused a chronic refractory infection in a patient with x-linked agammaglobulinemia; this infection persisted for > 2 years despite therapy with various antibiotics and immunoglobulins (igs). to characterize the defense status of this patient, several in vitro studies, including those with t cells and polymorphonuclear leukocytes (pmnls), were performed. t cell responses specific for c. jejuni were only weak in this patient. chemilumin ... | 1996 | 8879775 |
| effector mechanisms in demyelinating neuropathies. | while the cause of the immune-mediated neuropathies (guillain-barr-e syndrome, gbs; cidp; multi-focal motor neuropathy and paraproteinemic neuropathies) remains elusive considerable progress has been made in delineating the effector mechanisms that underlie myelin destruction. in gbs circulating antibodies to glycolipids possibly precipitated by an antecedent campylobacter jejuni infection can be detected with high frequency. these antibodies may interfere with neuromuscular transmission and eve ... | 1996 | 8881423 |
| [guillain-barré syndrome: development of concepts]. | the features initially described by guillain et barré have been updated through the proposal of precise criteria even if a number of questions remain. hundred of works have contributed to the evolution of these concepts. this paper is a summary of several opinions: 1. from nerve inflammation (lesions have been progressively analysed) to dysimmunity (with a combination of humoral and cellular dysimmunity), 2. the most frequent initial factors seems campylobacter jejuni with particular clinical co ... | 1996 | 8881425 |
| the guillain-barré syndrome; pathogenesis and treatment. | the guillain-barré syndrome (gbs) is an acute immune mediated polyneuropathy. diagnosis of clinically defined gbs is based upon symmetrical weakness developing within 4, but usually within 2 weeks and disappearance of myotatic reflexes. there is a large variability in clinical expression, including bulbar variants. also laboratory characteristics may vary. therefore, at present, diagnosis is based on the clinical parameters. in the individual patient characteristics of emg, immunological and mic ... | 1996 | 8881429 |
| isolation and characterisation of t lymphocytes from sural nerve biopsies in patients with guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy. | to characterise cultured t lymphocytes from nerve biopsies in patients with guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy (cidp). | 1996 | 8890774 |
| dirithromycin: a new macrolide. | to review the clinical microbiology and therapeutic use of dirithromycin, emphasizing comparative data between dirithromycin and the standard macrolide erythromycin, as well as clarithromycin and azithromycin. | 1996 | 8893122 |
| immunisation of chickens to reduce intestinal colonisation with campylobacter jejuni. | 1. systemic and intestinal antibody titres were measured in chickens following subcutaneous, intraperitoneal (i.p.), oral (p.o.) and combined i.p./p.o. administration of antigen, in soluble, emulsified or microparticulate form. antigens tested included keyhole limpet haemocyanin (klh), killed campylobacter jejuni whole cells and purified campylobacter flagellin protein. 2. the effect of immunisation with purified flagellin protein or with killed c. jejuni whole cells in reducing intestinal colon ... | 1996 | 8894221 |
| the lipooligosaccharides of pathogenic gram-negative bacteria. | lipooligosaccharides (loss) are the major glycolipids expressed on mucosal gram-negative bacteria, including members of the genera neisseria, haemophilus, bordetella, and branhamella. they can also be expressed on some enteric bacteria such as campylobacter jejuni and campylobacter coli strains. los is analogous to the lipopolysaccharide (lps) found in other gram-negative families. loss share similar lipid a structures with an identical array of functional activities as lpss. loss lack o-antigen ... | 1996 | 8894399 |
| the immunopathology of guillain-barré syndrome. | identification of new antigens in different patterns of guillain-barré syndrome has led to new pathophysiological concepts of guillain-barré syndrome and the related miller-fisher syndrome. patients with guillain-barré syndrome occurring after campylobacter jejuni infection have been found to develop more frequently axonal and motor forms of the syndrome. anti-gm1 antibodies decreased na+ current in the presence of complement. in acute axonal guillain-barré syndrome, macrophages were found in th ... | 1996 | 8894406 |
| host signal transduction and endocytosis of campylobacter jejuni. | caveolae are plasma membrane invaginations found in a variety of mammalian cells and are implicated in clathrin-independent endocytosis and signal transduction. here we show that pretreatment of caco-2 cell monolayers with filipin iii, which disrupts caveolae by chelating cholesterol, significantly reduces the ability of campylobacter jejuni to enter these cells. furthermore inhibitors of host protein tyrosine phosphorylation, the phosphatidylinositol-3 kinase (pl 3-kinase) inhibitor wortmannin, ... | 1996 | 8905618 |
| severe illness in african children with diarrhoea: implications for case management strategies. | to identify clinical disorders associated with severe illness in african children with diarrhoea, we studied a group of under-5-year-olds with diarrhoea who had been brought to a large public hospital in central cote d'ivoire. the general condition of children with diarrhoea was assessed and classified according to criteria recommended by who, and then used as a nonspecific indicator of severity. of the 264 children with diarrhoea who were enrolled in the study, 196 had nonsevere illness and 68 ... | 1995 | 8907771 |
| development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari. | contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ... | 1996 | 8914757 |
| enterotoxigenic enteric bacteria causing secretory diarrhoea. | two hundred and fifty enteric bacteria isolated from cases of secretory diarrhoea of all age groups were studied for their enterotoxigenicity and prevalence of drug resistance. the principal pathogens were escherichia coli 44.4%, vibrio cholerae 28.8%, salmonella typhimurium 19.2% and campylobacter jejuni 2.4%. 104 (42.6%) strains were enterotoxigenic; v. cholerae (100%), escherichia coli (25.2%) and non-e. coli enterobacteria (6.5%). while 89.3% and 100% enterotoxigenic escherichia coli and sal ... | 1995 | 8919105 |
| [colonic ulcers caused by campylobacter jejuni in a patient with hiv infection]. | 1996 | 8924483 | |
| [probable nature of rickettsia prowazekii virulence]. | a 29.5 kda outer membrane protein (ompb) of r. prowazekii virulent breinl strain is known to differ from its counterpart in attenuated madrid e strain, while ompb of this latter one and of its virulent variant evir coincide in mobility. the infectivity of these strains for macrophages was previously shown to be different as well, and to correlate with their virulence. previously cloned r. prowazekii breinl strain, 1.644-bp insert from lambda gtll recombinant expressing as ompb in inducer-indepen ... | 1996 | 8927063 |
| pathogenic micro-organisms in slaughterhouse sludge--a survey. | during slaughtering of animals and subsequent meat processing the process water used becomes polluted with organic matter of animal origin (i.e. protein and fat). this organic sludge is, in principle, a product suitable for animal feeding. to investigate the microbiological contamination level of sludge, raw sludge was collected at pig (n = 8) and poultry (n = 5) slaughterhouses. both flocculated and aerobically activated sludge was monitored. slaughterhouse sludge was heavily contaminated with ... | 1996 | 8930709 |
| characterization of campylobacter jejuni asd gene cloned in escherichia coli. | asd gene of the human enteric pathogen, campylobacter jejuni dz72/92, has been isolated from a genomic library constructed in the expression plasmid vector puc8-2 in escherichia coli. the gene has been fished out by complementation of the asd gene deletion present in the genome of e. coli chi 6097. the smallest recombinant plasmid (puwm3) able to confer asd+ phenotype contains a 1.8 kb insert cloned into hindiii site located within the multi-cloning site of the puc8-2 vector. the origin of the i ... | 1995 | 8934665 |
| the magnetic immuno-polymerase chain reaction assay for the detection of campylobacter in milk and poultry. | a rapid and sensitive technique, based on the magnetic immuno-polymerase chain reaction assay (mipa), was developed for the detection of campylobacter jejuni in milk and chicken products. target bacteria are captured from the food sample by magnetic particles coated with a specific antibody and the bound bacteria then lysed and subjected to pcr. the mipa could detect 420 cfu g-1 of chicken after 18 h, 42 cfu g-1 after 24 h, and 4.2 cfu g-1 after 36 h enrichment. for artificially contaminated mil ... | 1996 | 8934788 |
| [antimicrobial activities of clarithromycin against clinical isolates]. | to examine the antimicrobial activity of clarithromycin (cam) against strains clinically isolated from outpatients in 1994, minimum inhibitory concentrations (mics) were determined for cam and the control drugs. the results were as follows; 1. mic50 and mic90 of cam were similar to those investigated in 1980's against many bacterial species. 2. cam showed strong antimicrobial activities against beta-lactamase producing moraxella subgenus branhamella catarrhalis, bordetella pertussis, campylobact ... | 1996 | 8935125 |
| a novel polymerase chain reaction assay for the detection and speciation of thermophilic campylobacter spp. | a novel pcr amplification method is described which is specific for the thermophilic, enteropathogenic species campylobacter jejuni, camp. coli and camp. upsaliensis. rapid, accurate speciation of amplified strains is possible on the basis of restriction fragment length polymorphisms of pcr products digested with three restriction enzymes, alui, ddei and drai. the sensitivity of detection is 25 cfu in water, and 2 x 10(3) cfu in full cream milk. an epidemiological application of the assay in det ... | 1996 | 8939024 |
| differentiation of bacterial 16s rrna genes and intergenic regions and mycobacterium tuberculosis katg genes by structure-specific endonuclease cleavage. | we describe here a new approach for analyzing nucleic acid sequences using a structure-specific endonuclease, cleavase i. we have applied this technique to the detection and localization of mutations associated with isoniazid resistance in mycobacterium tuberculosis and for differentiating bacterial genera, species and strains. the technique described here is based on the observation that single strands of dnas can assume defined conformations, which can be detected and cleaved by structure-spec ... | 1996 | 8940459 |
| [hemolytic-uremic syndrome and campylobacter jejuni enteritis]. | 1996 | 8942950 | |
| murine intranasal challenge model for the study of campylobacter pathogenesis and immunity. | campylobacter jejuni infection of mice initiated by intranasal administration was investigated as a potential model for studies of pathogenesis and immunity. by using a standard challenge (5 x 10(9) cfu), c. jejuni 81-176 was more virulent for balb/c (72% mortality) than for c3h/hej (50%), cba/caj (30%), or c58/j (0%). intranasal challenge of balb/c was used to compare the relative virulence of three reference strains; c.jejuni 81-176 was more virulent (killing 83% of challenged mice) than c. je ... | 1996 | 8945529 |
| [campylobacter jejuni infections]. | 1996 | 8948834 | |
| [campylobacter jejuni infections in a prison population coinfected with the human immunodeficiency virus]. | campylobacter jejuni is a common etiological agent of diarrhea in the general population. in recent years it has also been involved as etiological agent of intestinal and extraintestinal infections in patients infected with the human immunodeficiency virus (hiv). the clinical and microbiological features in a series of hiv+ patients infected with c. jejuni in a correctional facility are here reported. | 1996 | 8948837 |
| microbial ecology of campylobacter jejuni in a united kingdom chicken supply chain: intermittent common source, vertical transmission, and amplification by flock propagation. | a study of campylobacter jejuni on a broiler chicken farm between 1989 and 1994 gave an estimated isolation rate of 27% (3,304 of 12,233) from a 0.9% sample of 1.44 million broiler chickens from six to eight sheds over 32 consecutive rearing flocks comprising 251 broiler shed flocks. during the study, c. jejuni was found in 35.5% of the 251 shed flocks but only 9.2% (23 of 251) had campylobacter isolates in successive flocks, with 9 of those 23 sheds having the same serotype between consecutive ... | 1996 | 8953731 |
| a combined polymerase chain reaction and restriction endonuclease enzyme assay for discriminating between campylobacter coli and campylobacter jejuni. | a combined polymerase chain reaction and restriction endonuclease (re) enzyme assay was developed to discriminate between campylobacter coli and campylobacter jejuni. amplimers of the flaa gene obtained by pcr were digested with alui and hinfi to distinguish c. coli from c. jejuni. with alui digestion c. jejuni-specific bands were observed at 110, 140 and 160 bp and c. coli-specific bands at 293 and 147 bp. c. jejuni-specific bands of 349 and 109 bp were found by hinfi digestion but hinfi did no ... | 1996 | 8954352 |
| affinity of anti-gm1 antibodies in guillain-barré syndrome patients. | in this study, we investigated the affinity of anti-gm1 igg antibodies as well as their igg subclass distribution in a series of 38 patients with guillain-barré syndrome. in 7 sera elevated titres of igg anti-gm1 antibodies could be detected. with respect to affinity there were two distinct groups of anti-gm1 antibodies: one group was of high affinity and did not cross-react with other glycolipids; the other group was of low affinity and cross-reacted with asialo-gm1. igg1 was the predominant an ... | 1996 | 8964918 |
| guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy: immune mechanisms and update on current therapies. | the relation between guillain-barré syndrome and chronic inflammatory demyelinating polyneuropathy is discussed. most likely they represent parts of a continuum, arbitrarily separated by their time course. within the concept of chronic inflammatory demyelinating polyneuropathy the presence of a monoclonal gammopathy of undetermined significance is discussed. the pathogenesis of inflammatory demyelinating polyneuropathies has not been elucidated yet, but involvement of the immune system has been ... | 1995 | 8968214 |
| microtitre plate riboprobe system for detection of ultrasonicated campylobacter jejuni genomic dna. | microtitre plate nucleic acid probe hybridization systems were developed for the detection of thermophilic campylobacter and campylobacter jejuni. specific rna probes obtained by in vitro transcription of dna templates synthesized by polymerase chain reaction using two sets of specific primers incorporating bacteriophage t7 promoter sequences were immobilized on a microtitre plate. the hybridizations were carried out on samples of genomic dna sheared by ultrasonication. optimum conditions for th ... | 1996 | 8972089 |
| heat-stable serotyping antigens expressed by strains of campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide. | the role of lipopolysaccharide (lps) in the serotyping of campylobacter jejuni based on heat-stable antigens was examined using sds-page and a silver stain for carbohydrate. none of the 32 type strains of camp. jejuni expressed long-chain lps. rabbit antibodies, prepared to 10 selected strains of camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not lps. this study suggests that the heat-stable antigens of camp. jejuni, which form the basis for the established penner s ... | 1996 | 8972090 |
| a milk-borne campylobacter outbreak following an educational farm visit. | after a nursery school trip to a dairy farm, 20 (53%) of 38 children and 3 (23%) of 13 adult helpers developed gastrointestinal infection. campylobacter jejuni was isolated from 15 primary cases and from 3 of 9 secondary household cases. a cohort study of the school party found illness to be associated with drinking raw milk (relative risk 5.4, 95% confidence interval 1.4-20.4, p = 0.001). there was a significant dose response relationship between amount of raw milk consumed and risk of illness ... | 1996 | 8972669 |
| the aroa gene of campylobacter jejuni. | the gene for 5-enolpyruvylshikimate-3-phosphate (epsp) synthase (aroa) cloned from campylobacter jejuni (cj) strain 81116 was identified by complementation of an escherichia coli (ec) auxotrophic aroa mutant. the cj aroa gene has been sequenced. it encodes an enzyme of 428 amino acids (aa), that is homologous to other bacterial epsp synthases, especially that of bacillus subtilis with which it has a 39% aa identity. the transcriptional start point was mapped. it is present in an upstream open re ... | 1996 | 8973316 |
| outer membrane characteristics of campylobacter jejuni grown in chickens. | a type of in vivo phenotype of campylobacter jejuni was obtained by maintaining bacteria in the peritoneal cavities of chickens for one week. these bacteria, which had not been subcultured on laboratory media, were used to prepare outer membranes for comparison with c. jejuni grown in vitro. flagella with subunits of 65 kda and a single porin with a protein subunit of 49 kda were expressed constitutively; however, outer membrane proteins of 55, 35 and 20 kda, and intermediate-chain lipopolysacch ... | 1996 | 8978102 |
| isolation of paramyxovirus serotype 7 from ostriches (struthio camelus). | paramyxovirus serotype 7 (pmv-7) was isolated from pooled intestinal contents of two 5-month-old ostriches (struthio camelus). the pathogenicity of the virus was comparable with lentogenic strains of newcastle disease virus (pmv-1) in chicken and chicken embryo pathogenicity tests. the relationship of the virus to the observed pathology of proliferative nonsuppurative enteritis is unknown; the campylobacter jejuni isolated was presumably the primary pathogen. to our knowledge, this is the first ... | 1996 | 8980831 |
| campylobacter jejuni peritonitis in a patient with liver cirrhosis. | a 56-year-old man with alcoholic liver cirrhosis (child-pugh class c), ascites and hepatocellular carcinoma developed acute diarrhoea and fever. ascites granulocyte count was 5760 per microliters. campylobacter jejuni grew in cultures from faeces, blood and ascites. the patient was successfully treated with erythromycin. although the incidence of bacterial infections including peritonitis is high in patients with end-stage liver cirrhosis, this is one of very few cases in which campylobacter jej ... | 1996 | 8980944 |
| electron microscopy of the major outer membrane protein of campylobacter jejuni. | the surfaces of the disrupted-cell surfaces of the campylobacter jejuni strains fum158432 and m1 were examined using the negative-staining technique and electron microscopy. the surfaces of the whole cells and the outer membranes were covered with small dark dots which, in some areas, were arranged in hexagonal patterns. the hexagonal arrangement was more clearly seen in extracted outer membrane. the size of each structure was measured based on a center-to-center distance with the adjacent struc ... | 1996 | 8981348 |
| antibody to galnac-gd1a and galnac-gm1b in guillain-barré syndrome subsequent to campylobacter jejuni enteritis. | n-acetylgalactosaminyl gd1a (galnac-gd1a) is a proposed target molecule for serum antibody in some patients with guillain-barré syndrome (gbs) (kusunoki et al., 1994). we examined autoantibody to galnac-gd1a in sera from 58 gbs patients. eight gbs patients had high igg anti-galnac-gd1a antibody titers, 3 of whom also had high igm anti-galnac-gd1a antibody titers. these 8 patients had experienced gastrointestinal infection before the onset of their neurological symptoms. campylobacter jejuni was ... | 1996 | 8982115 |
| [ecological and food safety considerations about products of vegetable origin]. | media have paid much attention in recent years to emerging microbiological problems in foods of plant origin. the potential for contamination of fruits and vegetables is high because of the wide variety of conditions to which produce is exposed during growth, harvest, processing and distribution. these considerations acquire great significance in the current scenario of the new processing techniques that offer attributes of convenience and fresh-likeness in response to changes in consumption pat ... | 1994 | 8984963 |
| [clinical evaluation of azithromycin in pediatric infections]. | azithromycin (azm) was studied for its clinical efficacy in pediatric infections. the study on azm was carried out in 43 patients whose diagnoses were given as follows: pharyngitis in five cases, tonsillitis in one, bronchitis in four, pneumonia in four, mycoplasma pneumonia in 14, scarlet fever in nine, impetigo in four, pyodermia in one and campylobacter enteritis in one. the patients received azm once daily at 1.6 approximately 20.0 mg/kg body weight for three to five days. effectiveness of a ... | 1996 | 8986554 |
| haemolytic-uraemic syndrome in the hunter: public health implications. | three cases of haemolytic-uraemic syndrome in the hunter area were reported in february 1995. an investigation was initiated to identify any verocytotoxic escherichia coli in clinical samples that could be associated with the development of the disease. escherichia coli o6:h- and o2:h7 were isolated from case 1. no organisms were identified for case 2, and case 3 samples yielded campylobacter jejuni. in addition, efforts were made to trace sources of any such pathogens in food samples or in the ... | 1996 | 8987212 |
| detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr. | pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ... | 1996 | 8987720 |
| molecular mimicry of host structures by bacterial lipopolysaccharides and its contribution to disease. | the core oligosaccharides of low-molecular-weight lipopolysaccharide (lps), also termed lipooligosaccharide (los), of pathogenic neisseria spp. mimic the carbohydrate moieties of glycosphingolipids present on human cells. such mimicry may serve to camouflage the bacterial surface from the host. the los component is antigenically and/or chemically identical to lactoneoseries glycosphingolipids and can become sialylated in neisseria gonorrhoeae when the bacterium is grown in the presence of cytidi ... | 1996 | 8988391 |
| characterization of campylobacter upsaliensis fur and its localization in a highly conserved region of the campylobacter genome. | despite increasing recognition of the importance of campylobacter upsaliensis in human disease little is known about either the virulence properties or genetics of this enteric pathogen. the complete coding sequence of a c. upsaliensis gene has yet to be published. we have cloned and sequenced the complete iron-uptake regulatory (fur) gene from the type strain of this species. the c. upsaliensis fur homolog was isolated from a genomic library of c. upsaliensis atcc 43954 constructed in phage lam ... | 1996 | 8996110 |
| [helicobacter pylori: antibodies in sera of pigs and calves]. | 64 sera of pigs and 22 sera of calves were investigated in a helicobacter pylori-elisa. the test system was validated with positive and negative reference sera. in contrast to other investigations it was tried to exclude false positive results by absorbing the sera with wolinella succinogenes, campylobacter jejuni subsp.jejuni, e. coli and proteus mirabilis. this proved to be necessary, as the extinctions of some sera were reduced considerably. using this technique sera of 5 pigs and 6 calves co ... | 1996 | 8999777 |
| igm anti-gm1 antibodies in the guillain-barré syndrome: a serological predictor of the clinical course. | it has been suggested that antibodies against gm1 are involved in the pathogenesis of the guillain-barré syndrome (gbs). recently, we have developed a standardized elisa assay for anti-gm1 antibodies of igm type well-suited for longitudinal patient studies. the relationship between serum antibodies against gm1 and campylobacter jejuni was investigated in patients with gbs and in patients with c. jejuni infection. patients with a short-lasting anti-gm1 elevation had a fast recovery, whereas patie ... | 1997 | 9003245 |
| application of the mast resistotyping scheme to campylobacter jejuni and c. coli. | the mast resistotyping scheme was assessed with 228 strains of campylobacter jejuni and c. coli from enteric infections in man and from a diverse selection of other sources (livestock, chickens and river water). most (153 of 158) c. jejuni examined were of the three most common penner (heat stable, hs) serotypes, hs1, hs2 and hs4 complex. fourteen resistotypes were identified in the 158 strains of c. jejuni and 16 in the 70 isolates of c. coli. the predominant codes were 00 (44% of c. jejuni; 33 ... | 1997 | 9003743 |
| [evolution of susceptibilities of campylobacter jejuni isolated from diarrhoeal cases to fluoroquinolones in tokyo]. | recently, the increase in the number of resistant strains of campylobacter jejuni to fluoroquinolone has been reported in european countries. we also studied antimicrobial susceptibilities of 600 clinical isolates of campylobacter jejuni isolated during a 6 year period from 1989 through 1994 in four tokyo metropolitan hospitals. the susceptibility to 6 antimicrobial agents, norfloxacin (nflx), ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), erythromycin (em) and tetracycline (tc) we ... | 1996 | 9011115 |
| [water-borne campylobacter infection--probably caused by pink-footed geese. two outbreaks in nord-trøndelag, stjørtdal in 1994 and verdal in 1995]. | the authors describe two water-borne outbreaks of campylobacter gastroenteritis that occurred in central norway in 1994 and 1995. the epidemics were probably caused by contamination of drinking water by the stools of pink-footed geese on the way from svalbard to germany-netherlands. campylobacter jejuni from the stools of the geese was transmitted to the population via untreated drinking water, causing disease in 50% of the population. about 1,000 persons suffered from gastroenteritis caused by ... | 1996 | 9011997 |
| rapid identification of campylobacter jejuni strains by polymerase chain reaction & their restriction fragment length polymorphism analysis. | a polymerase chain reaction (pcr) technique was developed for specific identification of c. jejuni. a primer pair of a conserved region of flagellin a (fla a) gene identified all 15 strains of c. jejuni isolated from human faeces. none of the control strains like helicobacter pylori, vibrio cholerae escherichia coli and salmonella typhimurium except c. coli exhibited any amplified product by pcr. a predicted 450 bp could also be amplified from 4 chicken caecal contents positive for c. jejuni-c c ... | 1997 | 9029829 |
| identification of a functional homolog of the escherichia coli and salmonella typhimurium cysm gene encoding o-acetylserine sulfhydrylase b in campylobacter jejuni. | the final step of l-cysteine biosynthesis in escherichia coli and salmonella typhimurium consists of the formation of l-cysteine from o-acetylserine and sulfide. this reaction can be catalyzed by two enzymes, o-acetylserine sulfhydrylase a and o-acetylserine sulfhydrylase b, the former of which has been more rigorously characterized. in contrast to o-acetylserine sulfhydrylase a, o-acetylserine sulfhydrylase b is preferentially used for cysteine biosynthesis during anaerobic growth and is able t ... | 1997 | 9034314 |
| direct detection of campylobacter jejuni in chicken cecal contents by pcr. | campylobacter jejuni in chicken feces was detected by pcr and southern blot hybridization (sbh). the detection limits of c. jejuni in chicken feces were 34,000 cells by pcr and 340 cells by sbh. some cecal contents of chickens up to 3 weeks old were c. jejuni positive by sbh whereas all of them were negative by pcr. two of 51 cecal contents of 18-day-old chicken embryos were c. jejuni positive by pcr and sbh; but, c. jejuni were not isolated from the samples by conventional culture with selectiv ... | 1997 | 9035088 |
| multifocal motor neuropathy with conduction block after campylobacter jejuni enteritis. | 1997 | 9040761 | |
| cross-reactive antibodies against gangliosides and campylobacter jejuni lipopolysaccharides in patients with guillain-barré or miller fisher syndrome. | campylobacter jejuni was isolated from stool specimens of 3 patients with miller fisher syndrome (mfs) and 2 patients with guillain-barré syndrome (gbs). anti-gq1b antibodies in serum from all mfs patients cross-reacted with sialidase-sensitive epitopes in the lipopolysaccharide fraction of c. jejuni from these 3 mfs patients. one gbs patient had anti-gm1 antibodies that bound with lipopolysaccharide of c. jejuni from a control patient and from the other gbs patient without anti-gm1 antibodies. ... | 1997 | 9041356 |
| specific identification of the enteropathogens campylobacter jejuni and campylobacter coli by using a pcr test based on the ceue gene encoding a putative virulence determinant. | a pcr method for the rapid identification and discrimination of thermophilic campylobacter jejuni and campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceue). a nucleotide sequence divergence of approximately 13% in the ceue genes of c. jejuni and c. coli facilitated the design of two species-specific pcr primer sets. the specificity of the pcr amplification reactions was confirmed by using two nonradioactively labelled species-specific intern ... | 1997 | 9041429 |
| n-nitrosation of medicinal drugs catalysed by bacteria from human saliva and gastro-intestinal tract, including helicobacter pylori. | micro-organisms commonly present in human saliva and three dsm strains (helicobacter pylori, campylobacter jejuni and neisseria cinerea), which can be isolated from the human gastro-intestinal tract, were assayed in vitro for their capacity to catalyse n-nitrosation of a series of medicinal drugs and other compounds. following incubation at ph 7.2 in the presence of nitrate (or nitrite) for up to 24 (48) h, the yield of n-nitroso compounds (noc) was quantified by hplc equipped with a post-column ... | 1997 | 9054633 |
| use of an arbitrarily primed pcr product in the development of a campylobacter jejuni-specific pcr. | development of a pcr assay for campylobacter jejuni is based on the isolation of species-specific dna. an arbitrarily primed pcr incorporating 10-mer primers was used to generate fingerprints of c. jejuni m129 genomic dna. fingerprint products were then screened individually for their species specificity in dot blot hybridizations with 6 c. jejuni isolates, 4 campylobacter species other than c. jejuni, and 27 enteric bacterial species other than campylobacter spp. a 486-bp fingerprint product hy ... | 1997 | 9055418 |
| cloning and nucleotide sequence of the gyra gene from campylobacter fetus subsp. fetus atcc 27374 and characterization of ciprofloxacin-resistant laboratory and clinical isolates. | the gyra gene of campylobacter fetus subsp. fetus, which encodes the a subunit of dna gyrase, was cloned, and its nucleotide sequence was determined. an open reading frame of 2,586 nucleotides which encodes a polypeptide of 862 amino acids with an mr of 96,782 was identified. c. fetus subsp. fetus gyra is most closely related to campylobacter jejuni gyra, with 73% homology at the nucleotide level and 78% identity between polypeptides. the next most closely related gyra was that from helicobacter ... | 1997 | 9056011 |
| campylobacter jejuni seasonal recovery observations of retail market broilers. | this study investigated possible seasonal trends in the campylobacter jejuni carrier state of market broilers. in this study, broiler carcasses, 15 each of two major companies, were obtained from a local supermarket each month for an entire year to evaluate the presence of c. jejuni on the carcasses. direct plating and the whole carcass rinse procedure were used for c. jejuni detection. resuscitation of damaged cells and preenrichment of low numbers of micoorganisms were accomplished by hunt's p ... | 1997 | 9057212 |
| reactivity of serum igg anti-gm1 ganglioside antibodies with the lipopolysaccharide fractions of campylobacter jejuni isolates from patients with guillain-barre syndrome (gbs). | campylobacter jejuni (cj) enteritis is the most frequently recognised infection preceding guillain-barre syndrome (gbs) and this combination is commonly associated with anti-gm1 ganglioside (anti-gm1) antibodies. we have examined the hypothesis that the anti-gm1 antibodies represent an immune response against the cj lipopolysaccharide (lps). we prepared the lps fraction from 8 isolates of cj, 3 from gbs patients with acute inflammatory demyelinating polyradiculoneuropathy (aidp), 3 from patients ... | 1997 | 9058756 |
| immunological investigation of chronic inflammatory demyelinating polyradiculoneuropathy. | in order to investigate the hypothesis that chronic inflammatory demyelinating polyradiculoneuropathy (cidp) is an autoimmune disease related to the acute inflammatory form of guillain-barre syndrome (gbs), we studied 40 patients, 40 age and sex matched controls with other forms of peripheral neuropathy (onp) and 37 controls from the same family or household (fc). we sought antibodies to gangliosides gm1 and lm1 by enzyme linked immunoassay (elisa) confirmed by immuno-overlay. only 6 (15%) cidp ... | 1997 | 9058768 |
| lineages within campylobacter jejuni defined by numerical analysis of pulsed-field gel electrophoretic dna profiles. | forty-seven penner heat-stable (hs) serotype reference strains for campylobacter jejuni and 47 serologically non-typable strains were examined by pulsed field gel electrophoresis (pfge) dna restriction analysis. the smai and kpni digest profiles were compared by numerical analysis. most strains grouped differently in the two analyses but strain lineages were inferred where the two agreed. genetic relationships between reference strains in the cross-reacting hs4 complex were examined. three clona ... | 1997 | 9060876 |
| motor nerve terminal degeneration provides a potential mechanism for rapid recovery in acute motor axonal neuropathy after campylobacter infection. | we investigated the possible mechanisms of paralysis and recovery in a patient with the acute motor axonal neuropathy (aman) pattern of the guillain-barré syndrome. the aman pattern of gbs is characterized clinically by acute paralysis without sensory involvement and electrodiagnostically by low compound motor action potential amplitudes, suggesting axonal damage, without evidence of demyelination. many aman patients have serologic or culture evidence of recent campylobacter jejuni infection. pa ... | 1997 | 9065554 |
| hydrophobic characterization of thermophilic campylobacter species and adhesion to int 407 cell membranes and fibronectin. | cell surface hydrophobicity of campylobacter jejuni, c. coli, c. lari and c. upsaliensis was tested by hydrophobic interaction chromatography on octylsepharose cl-4b. the hydrophobicity was influenced by cultivation mode, presence or absence of intact lipopolysaccharide (lps) and outer membrane protein structures. species-specific differences of hydrophobic characteristics were not detected. bacteria grown in fluid medium exhibited a high degree of hydrophobicity. agar-grown bacteria showed hydr ... | 1997 | 9075218 |
| chey-mediated modulation of campylobacter jejuni virulence. | four motile, non-adherent and non-invasive mutants of campylobacter jejuni 81-176 generated by a site-specific insertional mutagenesis scheme were characterized at the molecular level and all contained a duplication of the same region of the chromosome. when this region was cloned from wild-type 81-176 and transferred into 81-176 on a shuttle plasmid, the same non-invasive phenotype as the original mutants was observed, suggesting that the region contained a repressor of adherence and invasion. ... | 1997 | 9076738 |
| comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of campylobacter jejuni. | seventeen sporadic campylobacter jejuni enteritis cases occurred in taichung city, taiwan between july 1995 and september 1995. pulsed-field gel electrophoresis (pfge) and enterobacterial repetitive intergenic consensus (eric-1) primed polymerase chain reaction (pcr) techniques were compared for the epidemiological typing of the 17 c. jejuni isolates. fourteen distinct pfge fingerprint patterns were observed. fifteen distinct pcr fingerprint patterns were demonstrated. two clusters of isolates ( ... | 1996 | 9078444 |
| acute diarrhea in under five-year-old children admitted to king mongkut prachomklao hospital, phetchaburi province. | a prospective epidemiological and clinical study of acute diarrhea in children under 5 years old was done at king mongkut prachomklao hospital in order to provide baseline data for health officers to make a strategic plan to reduce the diarrheal mortality and morbidity, which is one of the mid-decade goals for children. there were 105 cases of acute diarrhea patients admitted to the pediatric ward between may 1995 and april 1996. seventy-six per cent of them were in the younger age group (> 1 mo ... | 1997 | 9078814 |