Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography. | molecular imaging of a suicide transgene's expression will aid the development of efficient and precise targeting strategies, and imaging for cancer cell viability may assess therapeutic efficacy. we used the pet reporter probe, 9-(4-[18f]fluoro-3-(hydroxymethyl)butyl)guanine ([18f]fhbg) to monitor the expression of a mutant herpes simplex virus 1 thymidine kinase (hsv1-sr39tk) in c6 glioma tumors implanted subcutaneously in nude mice that were repetitively being treated with the pro-drug gancic ... | 2005 | 15592447 |
| expression of cellular proteins bcl-x(l), xiap and bax involved in apoptosis in cells infected with herpes simplex virus 1 and effect of pavine alkaloid (-)-thalimonine on virus-induced suppression of apoptosis. | the expression of three cellular proteins involved in the modulation of apoptosis, namely antiapoptotic bcl-x(l) and xiap and proapoptotic bax, was investigated in cells infected with herpes simplex virus 1 (hsv-1). to assess whether the regulation of apoptosis in virus-infected cells depends on strain specificity the wild-type (wt) strain victoria and the mutant r-100 resistant to acyclovir (acv) were used. in addition, the expression of bcl-x(l), xiap and bax was studied in cells infected with ... | 2004 | 15595215 |
| herpes simplex virus-induced, death receptor-dependent apoptosis and regression of transplanted human cancers. | inoculation of a live attenuated herpes simplex virus (hsv) vector, betah1, into human u87mg glioblastoma cells transplanted into athymic nude mice induced complete regression of tumors. the infected cells underwent histochemically confirmed apoptosis without lymphocyte infiltration after expressing cd30, cd30 ligand (cd30l), tumor necrosis factor (tnf)-alpha, tnf receptor 1 (tnf-r1), fas, and fas ligand (fas-l) with activation of caspases 3 and 8. induction of the transcripts of these receptors ... | 2004 | 15596049 |
| identification of a region in the herpes simplex virus scaffolding protein required for interaction with the portal. | the herpes simplex virus type 1 capsid is a protective shell that acts as a container for the genetic material of the virus. after assembly of the capsid, the viral dna is translocated into the capsid interior through a channel formed by the portal. the portal is composed of a dodecamer of ul6 molecules which form a ring-like structure found at a single vertex within the icosahedron. formation of portal-containing capsids minimally requires the four structural proteins (vp5, vp19c, vp23, and ul6 ... | 2005 | 15596809 |
| association of viral factors with non-familial breast cancer in taiwan by comparison with non-cancerous, fibroadenoma, and thyroid tumor tissues. | to study the etiologic factors of non-familial breast cancer, the polymerase chain reaction (pcr) and southern hybridization were used to detect six viruses including human papillomavirus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2, and human herpesvirus (hhv)-8 dna in 69 patients with breast cancer and 60 specimens from non-cancerous or other individuals with thyroid tumors or fibroadenoma (non-breast cancer controls). two specimens from patients ... | 2005 | 15602723 |
| glycine-amide is an active metabolite of the antiretroviral tripeptide glycyl-prolyl-glycine-amide. | the chemically modified tripeptide glycyl-prolyl-glycine-amide (gpg-nh(2)) inhibits replication of human immunodeficiency virus (hiv) type 1 (hiv-1) in vitro, probably by interfering with capsid formation. the aim of the present study was to determine whether the metabolites glycyl-proline (gp-oh), glycine (g-oh), prolyl-glycine-amide (pg-nh(2)), proline (p-oh), and glycine-amide (g-nh(2)) from proteolytic cleavage may inhibit the replication of hiv-1 in vitro. pg-nh(2) has previously been shown ... | 2005 | 15616273 |
| efficient and non-toxic gene transfer to cardiomyocytes using novel generation amplicon vectors derived from hsv-1. | the feasibility of gene transfer to myocardial tissue using viral vectors was investigated over the last few years. in this study we report gene transfer using a recently described improved of herpes simplex virus (hsv-1)-derived amplicon vectors and demonstrate that these vectors are a powerful and potentially very interesting tool for gene transfer into neonatal primary as well as in adult cardiac myocytes. | 2004 | 15623439 |
| involvement of three mucous membranes in herpes-induced recurrent erythema multiforme. | 2005 | 15627109 | |
| a novel role for neutrophils as a source of t cell-recruiting chemokines ip-10 and mig during the dth response to hsv-1 antigen. | analogous to cd4+ t cells, neutrophils are essential participants in delayed-type hypersensitivity (dth) to herpes simplex virus type 1 antigen. however, what role they play in this cellular immune response is unclear. the recent recognition that neutrophils are potent producers of chemokines led us to hypothesize that they may help recruit cd4+ effector t cells. in the present study, we show that neutrophil depletion was accompanied by a marked decrease in the numbers of cd4+ and cxc receptor 3 ... | 2005 | 15629884 |
| antiviral effect of chinonin against herpes simplex virus. | in order to investigate the antiviral effect of chinonin against herpes simplex virus (hsv), the encephalitis model in mice and skin infection model in guinea pigs were established by hsv- i and hsv-ii infection respectively. acyclovir was used as the positive reference drug to evaluate the antiviral capacity of chinonin. chinonin showed an obvious therapeutic effect on encephalitis in mice at doses of 25 and 50 mg/kg. at both dosages, chinonin demonstrated stronger protection than acyclovir (1 ... | 2004 | 15641710 |
| "suicide" gene therapy of breast cancer cells is only cytostatic in vitro but anti-tumoral in vivo on breast mcf7-ras tumor. | gene therapy with herpes simplex virus thymidine kinase gene (hsv-tk) is effective in various tumor models in vitro and in vivo. we compared the efficacy of the hsv-tk gene therapy in vitro and in vivo in mcf-7 and mcf7-ras cells which form tumor in athymic mice. after viral infection, cells were treated with gcv (ganciclovir) and live cells were counted. the in vitro treatment significantly inhibited cell growth but did not induce early and late apoptosis, measured, respectively, by annexin or ... | 2004 | 15646826 |
| herpes simplex virus 1 infection activates the endoplasmic reticulum resident kinase perk and mediates eif-2alpha dephosphorylation by the gamma(1)34.5 protein. | the gamma(1)34.5 protein of herpes simplex virus (hsv) plays a crucial role in virus infection. although the double-stranded rna-dependent protein kinase (pkr) is activated during hsv infection, the gamma(1)34.5 protein inhibits the activity of pkr by mediating dephosphorylation of the translation initiation factor eif-2alpha. here we show that hsv infection also induces phosphorylation of an endoplasmic reticulum (er) resident kinase perk, a hallmark of er stress response. the virus-induced pho ... | 2005 | 15650164 |
| effective gene delivery to adult neurons by a modified form of electroporation. | non-viral methods of transfection of cdnas into adult neurons and other post-mitotic cells are generally very inefficient. however, the recent development of nucleofector technology developed by amaxa biosystems allows direct delivery of cdnas into the nucleus, enabling transfection of non-dividing cells. in this study, we describe a reliable method for culturing large numbers of retinal cells from adult rats and using nucleofection, we were able to transfect cdna-encoding gfp (jellyfish green f ... | 2005 | 15652627 |
| type-specific reactivity of anti-glycoprotein g antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues. | glycoprotein g-1 (gg-1) of herpes simplex virus type 1 (hsv-1) and gg-2 of hsv-2 are the only known hsv proteins that induce type-specific human antibody responses. recently, it was shown that purified human anti-gg-1 and anti-gg-2 antibodies presented a type-specific reactivity to immunogenic stretches with high similarity between gg-1 and gg-2. in this study, the molecular basis for this type-specific recognition was investigated employing synthetic peptides covering the indicated regions, inc ... | 2005 | 15659743 |
| combination of mutated herpes simplex virus type 1 (g207 virus) with radiation for the treatment of squamous cell carcinoma of the head and neck. | g207 is an oncolytic herpes simplex virus (hsv) with deletions at both gamma134.5 loci and a lacz gene insertion inactivating the hsv ribonucleotide reductase gene. ionising radiation induces the growth arrest-inducible gene, gadd34, and ribonucleotide reductase. gadd34 is a protein that correlates with apoptosis following radiation and has homology with the g207 gamma134.5 gene. we hypothesised that the combination of radiotherapy with g207 may have a potentiating effect on viral replication an ... | 2005 | 15661558 |
| new conformationally locked bicyclic n,o-nucleoside analogues of antiviral drugs. | in order to obtain rigidity within the sugar moiety of nucleosides, the bicyclic pyrimidine derivatives of n,o-isoxazolidines were designed and synthesized by using 1,3-dipolar cycloaddition of delta(1)-pyrrolidine-1-oxide and the appropriate vinyl-nucleobases. | 2005 | 15664810 |
| the herpes simplex virus type 1 dna polymerase processivity factor, ul42, does not alter the catalytic activity of the ul9 origin-binding protein but facilitates its loading onto dna. | the herpes simplex virus type 1 ul42 dna polymerase processivity factor interacts physically with ul9 and enhances its ability to unwind short, partially duplex dna. in this report, atp hydrolysis during translocation of ul9 on single-stranded (ss) or partially duplex dna was examined in the presence and absence of ul42 to determine the effect of ul42 on the catalytic function of ul9. our studies reveal that a homodimer of ul9 is sufficient for dna translocation coupled to atp hydrolysis, and th ... | 2005 | 15673714 |
| an association of herpes simplex virus type 1 infection with type 2 diabetes. | 2005 | 15677810 | |
| human dendritic cells transduced with herpes simplex virus amplicons encoding human immunodeficiency virus type 1 (hiv-1) gp120 elicit adaptive immune responses from human cells engrafted into nod/scid mice and confer partial protection against hiv-1 challenge. | small-animal models are needed to test human immunodeficiency virus (hiv) vaccine efficacy following viral challenge. to this end, we examined hiv-1-specific immune responses following immunization of nonobese diabetic-severe combined immunodeficient mice that were repopulated with human peripheral blood lymphocytes (hu-pbl-nod/scid mice). autologous dendritic cells (dc) were transduced ex vivo with replication-defective, helper virus-free, herpes simplex virus type 1 (hsv-1) amplicons that expr ... | 2005 | 15681415 |
| role of natural killer t (nkt) cells lacking interleukin (il)-4 producing abilities on the cc-chemokine ligand 2-associated herpes simplex virus type 1 infection in human severe combined immunodeficiency (scid) mouse chimeras. | cc-chemokine ligand 2 (ccl2/monocyte chemoattractant protein 1) is known to have an important role on t helper type 2 (th2) cell generation and described to induce interleukin (il)-4 production by activated t cells. in the present study, an increase of ccl2 production in cultures of peripheral blood lymphocytes (pbl) from patients with severe thermal injuries was demonstrated. severe combined immunodeficiency (scid) mice reconstituted with pbl from healthy donors (pbl-scid chimeras) were resista ... | 2005 | 15683684 |
| rapid diagnosis of herpes simplex virus infection by a loop-mediated isothermal amplification method. | primers for herpes simplex virus type 1 (hsv 1)-specific loop-mediated isothermal amplification (lamp) method amplified hsv-1 dna, while hsv-2-specific primers amplified only hsv-2 dna; no lamp products were produced by reactions performed with other viral dnas. the sensitivities of the hsv-1- and hsv-2-specific lamp methods, determined by agarose gel electrophoresis, reached 500 and 1,000 copies/tube, respectively. the turbidity assay, however, determined the sensitivity of the hsv-1- and hsv-2 ... | 2005 | 15695716 |
| bell's palsy and herpes simplex virus: fact or mystery? | in recent years, progress has been made in the understanding of bell's palsy, the most common form of acute facial weakness. herpes simplex virus (hsv) reactivation within the geniculate ganglion with subsequent inflammation and entrapment of the nerve at the meatal foramen has been proposed to be the pathogenetic mechanism. we challenged its accuracy by analyzing our own data on the presence of viral genomic dna of hsv-1 and 2, human herpes virus (hhv)-6a/b, as well as varizella zoster virus (v ... | 2005 | 15699730 |
| the ectodomain of herpes simplex virus glycoprotein h contains a membrane alpha-helix with attributes of an internal fusion peptide, positionally conserved in the herpesviridae family. | human herpesviruses enter cells by fusion with target membranes, a process that requires three conserved glycoproteins: gb, gh, and gl. how these glycoproteins execute fusion is unknown. neural network bioinformatics predicted a membrane alpha-helix contained within the ectodomain of herpes simplex virus (hsv) gh, positionally conserved in the gh of all examined herpesviruses. evidence that it has attributes of an internal fusion peptide rests on the following lines of evidence. (i) the predicte ... | 2005 | 15709012 |
| vesiculobullous lesion on the right fifth toe. | 2005 | 15714680 | |
| phenolics with antiviral activity from millettia erythrocalyx and artocarpus lakoocha. | from the leaves of millettia erythrocalyx, a new flavone named 3',5'-dimethoxy-[2",3": 7,8]-furanoflavone and three known compounds were isolated. assays for anti-herpes simplex virus activity (hsv-1 and hsv-2) were performed on 24 phenolic compounds obtained from m. erythrocalyx and artocarpus lakoocha. it was found that the flavones ovalifolin, pongol methyl ether and millettocalyxin a, and the stilbene oxyresveratrol possessed moderate activity against both types of hsv. in addition, oxyresve ... | 2005 | 15715263 |
| possible mechanism underlying the antiherpetic activity of a proteoglycan isolated from the mycelia of ganoderma lucidum in vitro. | glpg (ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of ganoderma lucidum, etoh precipitation, and deae-cellulose column chromatography.glpg was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. its antiviral activities against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using a cytopathic inhibition assay. glpg inhibited cell death in a dose-dependent manner in hsv-infected cells. in addition, ... | 2005 | 15715944 |
| extensive primary cutaneous herpes simplex virus type 1 infection in an infant following acute rotavirus gastroenteritis. | 2004 | 15717179 | |
| multimutated herpes simplex virus g207 is a potent inhibitor of angiogenesis. | the mode of the antitumoral activity of multimutated oncolytic herpes simplex virus type 1 g207 has not been fully elucidated yet. because the antitumoral activity of many drugs involves the inhibition of tumor blood vessel formation, we determined if g207 had an influence on angiogenesis. monolayers of human umbilical vein endothelial cells and human dermal microvascular endothelial cells, but not human dermal fibroblasts, bronchial epithelial cells, and retinal glial cells, were highly sensiti ... | 2004 | 15720798 |
| regulation of ctl responses to mhc-restricted class i peptide of the gp70 tumour antigen by splenic parenchymal cd4+ t cells in mice failing immunotherapy with disc-mgm-csf. | direct intratumour injection of the disabled infectious single-cycle-herpes simplex virus-encoding murine granulocyte/macrophage colony-stimulating factor (disc-hsv-mgm-csf) into established colon carcinoma ct26 tumours induced complete tumour rejection in up to 70% of treated animals (regressors), while the remaining mice developed progressive tumours (progressors). this murine balb/c model was used to dissect the cellular mechanisms involved in tumour regression or progression following immuno ... | 2005 | 15723299 |
| liver-directed therapies in colorectal cancer. | the liver is the most common site of metastatic colorectal cancer (crc) and the status of this organ is an important determinant of overall survival in patients with advanced disease. complete resection of hepatic crc metastases can provide a long-term cure for some patients, but the majority of liver metastases are not amenable to such surgery. furthermore, most patients after curative resection ultimately suffer from recurrence, and the majority of such failures occur in the liver. various abl ... | 2005 | 15726510 |
| fluorosubstitution and 7-alkylation as prospective modifications of biologically active 6-aryl derivatives of tricyclic acyclovir and ganciclovir analogues. | a series of fluorine containing tricyclic analogues of acyclovir (acv, 1) and ganciclovir (gcv, 2) were synthesized and evaluated for their activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and cytostatic activity against hsv-1 thymidine kinase (tk) gene-transduced human osteosarcoma tumour cells. it was found that fluorine substitution reduced the antiviral activity, but most of the new compounds were pronounced cytostatic agents with potency and selectivity similar to tho ... | 2005 | 15727862 |
| identification of an essential domain in the herpes simplex virus 1 ul34 protein that is necessary and sufficient to interact with ul31 protein. | previous results have indicated that the herpes simplex virus 1 ul31 and ul34 proteins interact and form a complex at the inner nuclear membranes of infected cells, where both play important roles in the envelopment of nucleocapsids at the inner nuclear membrane. in the work described here, mapping studies using glutathione s-transferase pull-down assays indicated that amino acids 137 to 181 of the ul34 protein are sufficient to mediate an interaction with the ul31 protein. a recombinant virus ( ... | 2005 | 15731273 |
| evaluation of some pyrazoloquinolines as inhibitors of herpes simplex virus type 1 replication. | three structurally related aminopyrazoloquinoline derivatives were evaluated for their antiviral activity against herpes simplex virus type 1. these compounds were examined for their in vitro antiviral activity by two different bioassays, namely; crystal violet staining and tetrazolium dye (mts) measurement. the antiviral role of these compounds was confirmed by enumerating the infectious particles with plaque assay. the acute toxicity values of the biologically active compounds were determined ... | 2005 | 15736285 |
| orientation determination by wavelets matching for 3d reconstruction of very noisy electron microscopic virus images. | in order to perform a 3d reconstruction of electron microscopic images of viruses, it is necessary to determine the orientation (euler angels) of the 2d projections of the virus. the projections containing high resolution information are usually very noisy. this paper proposes a new method, based on weighted-projection matching in wavelet space for virus orientation determination. in order to speed the retrieval of the best match between projections from a model and real virus particle, a hierar ... | 2005 | 15743519 |
| [herpesvirus infection in patients with chronic glomerulonephritis]. | virological examinations of blood, urine and saliva in 75 patients with chronic glomerulonephritis (cg) revealed, in 95% of them, herpes-virus infections caused by herpes simplex virus, type 1 (34.4%), herpes simplex virus, type 2 (2.6%) and cytomegalovirus (12%) or mixed infections (46%). the infection rate in the control group of children without renal pathology was reliably lower. a majority of cg patients (94%) had a diagnostically significant level of antiherpetic antibodies, class igg, whi ... | 2005 | 15747870 |
| a dna recombination-based approach to eliminate papillomavirus infection. | at present, no treatments exist that effectively target and eliminate papillomaviruses (pvs) from infected cells or prevent its replication. we are employing a strategy to prevent virus replication in pv-infected cells through the conditional expression of the herpes simplex virus type 1 thymidine kinase (tk) gene. expression of tk in this system is expected to be triggered by a homologous recombination event between the endogenous pv genome and a nonexpressing tk gene cassette. recombination be ... | 2005 | 15756291 |
| avian adenovirus vector celo-tk displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors. | avian adenovirus celo is a novel adenovirus vector system with the advantages of efficient production, high virion stability, and the absence of crossreactivity with ad5-neutralizing antibodies. in this study, we evaluated the anticancer efficacy of a celo vector encoding the herpes simplex virus type 1 thymidine kinase, a prodrug-activating therapeutic gene. vectors carrying the gene for hsv-tk or egfp under the control of the hcmv promoter in place of the "nonessential" region of the celo geno ... | 2005 | 15761475 |
| herpes simplex virus targeting to the egf receptor by a gd-specific soluble bridging molecule. | herpes simplex virus 1 (hsv-1) enters cells via initial binding of envelope glycoproteins (g) c and b to cell-surface glycosaminoglycans (gags) and subsequent membrane fusion involving envelope gd, gb, and gh/gl. current insights suggest that the fusion process is initiated by interaction of gd with a cognate cellular receptor, such as the widely distributed cell adhesion molecule nectin-1. to redirect the tropism of hsv-1, we have generated a soluble adapter protein (p-v528lh) comprising the gd ... | 2005 | 15771964 |
| [molecular genetic analysis of thimidine kinase from herpes simplex virus type 1]. | 2005 | 15773560 | |
| association of human endogenous retroviruses with multiple sclerosis and possible interactions with herpes viruses. | the hypothesis that human endogenous retroviruses (hervs) play a role in autoimmune diseases is subject to increasing attention. hervs represent both putative susceptibility genes and putative pathogenic viruses in the immune-mediated neurological disease multiple sclerosis (ms). gammaretroviral herv sequences are found in reverse transcriptase-positive virions produced by cultured mononuclear cells from ms patients, and they have been isolated from ms samples of plasma, serum and csf, and chara ... | 2005 | 15782388 |
| mathematical analysis demonstrates that interferons-beta and -gamma interact in a multiplicative manner to disrupt herpes simplex virus replication. | several studies suggest that the innate interferons (ifns), ifn-alpha and ifn-beta, can act in concert with ifn-gamma to synergistically inhibit the replication of cytomegalovirus and herpes simplex virus type 1 (hsv-1). the significance of this observation is not yet agreed upon in large part because the nature and magnitude of the interaction between ifn-alpha/beta and ifn-gamma is not well defined. in the current study, we resolve this issue by demonstrating three points. first, the hyperboli ... | 2005 | 15784277 |
| cytokine profiles and inflammatory cells during hsv-1-induced acute retinal necrosis. | to investigate infiltrating cells, cytokines, and kinetics of cytokine expression during acute retinal necrosis (arn) in the uninoculated eye after inoculation of herpes simplex virus (hsv)-1 into the anterior chamber of one eye of balb/c mice. | 2005 | 15790902 |
| a branched, synthetic oligopeptide corresponding to a region of glycoprotein g of hsv-1 reacts sensitively and specifically with hsv-1 antibodies in an elisa. | herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), which are common worldwide, are so similar that antibodies directed against one serotype may crossreact with antigens from the other one. methods for specific detection of antibodies against hsv-1 or hsv-2 are based upon the antigenicities of glycoproteins g. however, due to the cost, the available commercial methods may not readily be used in developing countries. a different enzyme-linked immunosorbent assay (elisa) method, based upon a s ... | 2005 | 15794983 |
| a systematic review of the epidemiology and interaction of herpes simplex virus types 1 and 2. | to explore epidemiological evidence about the interaction of herpes simplex virus (hsv) 1 and hsv-2 infections. | 2005 | 15800084 |
| primary cutaneous plasmacytoma: a role for a triggering stimulus? | primary cutaneous plasmacytoma is a rare type of cutaneous b-cell lymphoma, characterized by clonal proliferation of plasma cells, that primarily develops in the skin. five cases have been described to date in which a local triggering stimulus may be involved in development of this skin tumour. we describe the case of a primary cutaneous plasmacytoma localized to the lower lip. this site had been affected for 15 years with recurrent herpes simplex virus-1 infection. neoplastic plasma cells were ... | 2005 | 15807675 |
| cd8+-dependent cns demyelination following ocular infection of mice with a recombinant hsv-1 expressing murine il-2. | demyelinating diseases comprise a spectrum of immunopathologic syndromes in which myelin, the fatty covering of nerve cell fibers in the brain and spinal cord, is destroyed. in this study, we have shown for the first time that ocular infection of balb/c mice with a recombinant herpes simplex virus type 1 (hsv-1) expressing il-2 (hsv-il-2) results in cns demyelination as determined by light microscopy and em. the demyelinated lesions involve periventricular white matter, brain stem, and spinal co ... | 2005 | 15817260 |
| dna polymerase gene locus of cercopithecine herpesvirus 1 is a suitable target for specific and rapid identification of viral infection by pcr technology. | the family herpesviridae comprises at least 100 herpesviruses. numerous human and animal pathogenic herpesviruses have been identified so far, including cercopithecine herpesvirus 1 (cehv-1). this virus is a member of the subfamily alphaherpesvirinae and is the most hazardous herpesvirus to man. cehv-1 is also known as b-virus or monkey b virus and as herpesvirus simiae. in order to gain more genetic information, the viral dna polymerase (dpol) gene was identified using polymerase chain reaction ... | 2005 | 15830148 |
| study of the viral infections and cytokines associated with recurrent aphthous ulceration. | mouth ulcers are one of the most common oral complaints. however, the association between oral ulceration and viruses and cytokines is uncertain. we detected the presence of human papilloma virus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2 and human herpesvirus (hhv)-8 dna in oral tissues by polymerase chain reaction (pcr) and southern hybridization techniques, and quantified the serum levels of cytokines including interleukin (il)-2, il-4, granulo ... | 2005 | 15840465 |
| detection and typing of herpes simplex dna in genital swabs by real-time polymerase chain reaction. | the lightcycler polymerase chain reaction (pcr) is a sensitive assay for the detection of herpes simplex virus (hsv) dna in muco-cutaneous swabs. software-based analysis of the probe melting temperature (tm) can be used to discriminate between hsv types (hsv-1 and hsv-2). among 76 hsv dna positive genital swabs, atypical tms were observed in 14 (18%). the 14 samples were all typed as hsv-2 by sequence alignment. in 4/14 samples, the atypical tm was associated with sequence variation at the probe ... | 2005 | 15847938 |
| dynamics of the antiviral activity of n-methanocarbathymidine against herpes simplex virus type 1 in cell culture. | n-methanocarbathymidine [(n)-mct], a thymidine analogue, exhibits potent activity in cell culture against herpes simplex virus1 (hsv-1). (n)-mct showed higher antiviral activity than ganciclovir (gcv). continuous treatment of vero cells with (n)-mct immediately or 10 h post-infection (p.i.) fully prevented the development of viral infection. however, when infected cells were treated with (n)-mct at 12 h p.i., there was only a partial inhibition (ca. 50%). additionally, continuous treatment of in ... | 2005 | 15848299 |
| lethal encephalitis in myeloid differentiation factor 88-deficient mice infected with herpes simplex virus 1. | herpes simplex virus 1 (hsv-1), a large dna virus from the herpesviridae family, is the major cause of sporadic lethal encephalitis and blindness in humans. recent studies have shown the importance of toll-like receptors (tlrs) in the immune response to hsv-1 infection. myeloid differentiation factor 88 (myd88) is a critical adaptor protein that is downstream to mediated tlr activation and is essential for the production of inflammatory cytokines. here, we studied the relationship between myd88 ... | 2005 | 15855642 |
| the role of viral and host genes in corneal infection with herpes simplex virus type 1. | herpes simplex virus infection of the eye is the leading cause of blindness due to infection in the us despite the availability of several antiviral drugs. studies with animal models have shown that three factors, innate host resistance, the host adaptive immune response, and the strain of virus interact to determine whether an infection is asymptomatic or proceeds to the development of blinding keratitis (hsk). of these, the role of adaptive immunity has received the most attention. this work h ... | 2005 | 15862167 |
| [reactivation of persistent herpes virus infection as a factor of endogenous uveitis in children]. | two hundred and fifty-five children with endogenous uveitis, aged 2 months to 15 years, were examined. 73-93% of children were chronically infected by different viruses of the human herpes group; mycoplasmal past-infection was detected in only 13% of them and chlamydia past-infection _ in 3.7% of them. herpes simplex virus of type 1 reactivated reliably more often versus other types of herpesviridae. reactivation of cytomegalovirus infection prevailed in mothers who gave birth to children with i ... | 2005 | 15881151 |
| antiviral evaluation of plants from brazilian atlantic tropical forest. | the antiviral activity of six medicinal plants from brazilian atlantic tropical forest was investigated against two viruses: herpes simplex virus type 1 (hsv-1) and poliovirus type 2 (pv-2). cuphea carthagenensis and tillandsia usneoides extracts showed the best antiherpes activity. t. usneoides dichloromethane, ethyl acetate and n-butanol extracts, and lippia alba n-butanol extract showed inhibition of hsv-1, strain 29r/acyclovir resistant. in addition, only l. alba ethyl acetate extract showed ... | 2005 | 15890472 |
| the carboxyl-terminal domain of rna polymerase ii is phosphorylated by a complex containing cdk9 and infected-cell protein 22 of herpes simplex virus 1. | the infected-cell protein 22 (icp22), a regulatory protein encoded by the alpha22 gene of herpes simplex virus 1, is required for the optimal expression of a set of late viral proteins that includes the products of the u(s)11, u(l)38, and u(l)41 genes. icp22 has two activities. thus, icp22 and the u(l)13 protein kinase mediate the activation of cdc2 and degradation of its partners, cyclins a and b. cdc2 and its new partner, the dna polymerase accessory factor (u(l)42), bind topoisomerase iialpha ... | 2005 | 15890914 |
| herpes simplex virus type 1 ul51 protein is involved in maturation and egress of virus particles. | the ul51 gene of herpes simplex virus type 1 (hsv-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. recently, we reported that ul51 protein colocalizes with golgi marker proteins in transfected cells and that targeting of ul51 protein to the golgi apparatus depends on palmitoylation of its n-terminal cysteine at position 9 (n. nozawa, t. daikoku, t. koshizuka, y. yamauchi, t. yoshikawa, and y. nishiyama, j. virol. 77:3204-3216, 2003). however, its role ... | 2005 | 15890934 |
| activation of c-jun n-terminal kinase (jnk) pathway by hsv-1 immediate early protein icp0. | the immediate early protein icp0 encoded by herpes simplex virus 1 (hsv-1) is believed to activate transcription and consequently productive infection. the precise mechanisms of icp0-mediated transactivation are under intensive study. here, we demonstrate that icp0 can strongly activate ap-1 responsive genes specifically. this activation is inhibited by c-jun (s73a), c-jun (s63/73a), tak1 (k63w), but not by p38 (af), erk1 (k71r), erk2 (k52r) and traf6 (c85a/h87a). we further investigate the rele ... | 2005 | 15896775 |
| components of the rest/corest/histone deacetylase repressor complex are disrupted, modified, and translocated in hsv-1-infected cells. | the infected cell protein (icp)0 enables gene expression and the replication of herpes simplex virus (hsv)-1 in cells infected at low multiplicities and enhances the expression of genes introduced into cells by transfection or infection. we report that a short sequence of icp0 is similar to a sequence in the amino terminus of corest, a corepressor that exists in complexes with the repressor rest and histone deacetylases (hdacs) 1 or 2 to repress cellular gene expression. in wild-type-virus-infec ... | 2005 | 15897453 |
| enhanced efficiency and specificity of ovarian cancer gene therapy in rats with a novel nonviral gene delivery system (ge7) via intraovarian artery perfusion approach. | transfer of the herpes simplex virus type i-thymidine kinase gene, followed by the administration of ganciclovir (hsv1-tk/gcv) into ovarian cancer-derived cell line either in vitro or transplanted into nude mice has been shown to provide a potential strategy for the gene therapy of ovarian cancer. we investigated the antitumor effects of hsv1-tk/gcv strategy with a chemically induced rat ovarian cancer model and a tumor-selective gene delivery by a novel nonviral gene delivery system (ge7) throu ... | 2005 | 15905861 |
| inhibition of herpes simplex virus thymidine kinases by 2-phenylamino-6-oxopurines and related compounds: structure-activity relationships and antiherpetic activity in vivo. | derivatives of the herpes simplex thymidine kinase inhibitor hbpg [2-phenylamino-9-(4-hydroxybutyl)-6-oxopurine] have been synthesized and tested for inhibitory activity against recombinant enzymes (tk) from herpes simplex types 1 and 2 (hsv-1, hsv-2). the compounds inhibited phosphorylation of [3h]thymidine by both enzymes, but potencies differed quantitatively from those of hbpg and were generally greater for hsv-2 than hsv-1 tks. changes in inhibitory potency were generally consistent with th ... | 2005 | 15916444 |
| the herpes simplex virus icp27 protein: a multifunctional post-transcriptional regulator of gene expression. | the herpes simplex virus 1 icp27 is an essential, highly conserved protein involved in various steps of herpes simplex virus 1 gene regulation as well as in the shut-off of host gene expression during infection. it functions primarily at the post-transcriptional level in inhibiting precursor mrna splicing and in promoting nuclear export of viral transcripts. these activities are discussed. | 2005 | 15916551 |
| acute hemorrhagic leukoencephalitis manifesting as intracerebral hemorrhage associated with herpes simplex virus type i. | acute hemorrhagic leukoencephalitis (ahl) is a rare and usually fatal disorder characterized by an acute onset of neurological abnormalities. it may occur in association with a viral illness or vaccination. radiology and brain biopsy are essential for the diagnosis. we present a case of ahl mimicking or manifesting as intracerebral hemorrhage associated with herpes simplex virus. a 3-year-old boy was admitted to our hospital with cerebral hemorrhage. the findings of cerebrospinal fluid, mri and ... | 2005 | 15917264 |
| herpes simplex virus 1 inhibits apoptosis through a caspase-3-dependent pathway in primary cultures of cortical neuronal cells of fetal mice. | we could induce apoptosis in primary cultures of cortical neurons of fetal mice with ceramide or sorbitol. the induction was accompanied by an increase in caspase-3 (cas-3) activity and depolarization of the inner mitochondrial membrane of neuronal cells which both could be reversed by herpes simplex virus 1 (hsv-1) infection. we conclude tha hsv-1 infection inhibited the apoptosis, induced in neuronal cells by sorbitol or ceramide, via a cas-dependent pathway. | 2005 | 15929398 |
| implications for herpes simplex virus vaccine strategies based on antibodies produced to herpes simplex virus type 1 glycoprotein gc immune evasion domains. | herpes simplex virus type i (hsv-1) glycoprotein gc (gc-1) is an immune evasion molecule that inhibits complement activation by binding c3b. three assays were used to assess whether igg antibodies produced by hsv-1 infection in humans block the interaction between c3b and gc-1. in two assays human igg had no effect, while in one assay igg partially inhibited c3b binding, which occurred at igg concentrations approaching the upper limits of those found in human serum. mice infected with hsv-1 prod ... | 2005 | 15936852 |
| induction of suppressor of cytokine signaling-3 by herpes simplex virus type 1 confers efficient viral replication. | we showed previously that infection of herpes simplex virus type 1 (hsv-1) rapidly induced the suppressor of cytokine signaling-3 (socs3), a host negative regulator of the jak/stat pathway, in the amnion cell line fl. thus, hsv-1 suppresses the interferon (ifn) signaling pathway at the step of ifn-induced phosphorylation of janus kinases during an early infection stage. in the present study, we examined socs3 induction by hsv-1 infection in several types of human cell lines. fl cells and the t-c ... | 2005 | 15939448 |
| role of oxidative damage in the pathogenesis of viral infections of the nervous system. | oxidative stress, primarily due to increased generation of reactive oxygen species (ros) and reactive nitrogen species (rns), is a feature of many viral infections. ros and rns modulate the permissiveness of cells to viral replication, regulate host inflammatory and immune responses, and cause oxidative damage to both host tissue and progeny virus. the lipid-rich nervous system is particularly susceptible to lipid peroxidation, an autocatalytic process that damages lipid-containing structures an ... | 2005 | 15944946 |
| epidemiological, clinical and laboratory aids for the diagnosis of neonatal herpes -- an australian perspective. | neonatal herpes is rare in australia, possibly because of the older average age at pregnancy compared with other developed countries, low herpes simplex virus (hsv)-2 seroprevalence in the general community, low risk of hsv-2 acquisition during pregnancy and relatively high hsv-1 seroprevalence in adults. guidelines on herpes management in pregnancy have been produced by the australian society for infectious diseases and endorsed by the australian college of obstetricians and gynaecologists. how ... | 2004 | 15955266 |
| inhibition of dna repair by a herpes simplex virus vector enhances the radiosensitivity of human glioblastoma cells. | expression of the herpes simplex virus (hsv) protein, icp0, from the viral genome, rendered two radioresistant human glioblastoma multiforme cell lines more sensitive to the effects of ionizing radiation. using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and clonogenic survival assays, u87-mg and t98 cell survival was more greatly decreased as a function of ionizing radiation dose when icp0 was preexpressed in cells compared with when icp0 was not expressed. consistent with ... | 2005 | 15958578 |
| analysis of a bovine herpesvirus 1 recombinant virus that does not express the bicp0 protein. | bovine herpesvirus 1 (bhv-1) infected-cell protein 0 (bicp0) stimulates productive infection by activating viral gene expression. in this study, an attempt was made to construct a recombinant virus with point mutations in the c(3)hc(4) zinc ring finger of bicp0, as this domain is necessary for activating viral transcription and productive infection. a virus was identified in bovine cells that induced small clusters of infected cells resembling a small plaque. instead of the expected mutations wi ... | 2005 | 15958678 |
| a microarray study to characterize the molecular mechanism of timp-3-mediated tumor rejection. | glial cell invasion is a multistep cellular process that involves a complex system of tightly regulated proteases (matrix metalloproteinases; mmps) and their endogenous inhibitors (tissue inhibitors of metalloproteinases; timps) to mediate the degradation of the basement membrane and extracellular matrix. tissue inhibitor of metalloproteinases-3 (timp-3) is a matrix-bound inhibitor of mmps. in the present study, we have overexpressed the timp3 gene in human glioma cells with a herpes simplex vir ... | 2005 | 15963930 |
| involvement of cellular death signals in the reactivation of herpes simplex virus type 1 and lambda bacteriophage from a latent state. | herpes simplex virus (hsv) 1 has adapted to the human host through two modes of infection, the acute-transient infection that may cause diseases (such as encephalitis) and the latent state, which is a source for recurrent infection and disease. while much information has been gathered on the cellular and molecular concomitants of establishment and maintenance of hsv-1 latent state, the biological basis of viral reactivation is still unclear. despite their obvious differences, hsv-1 and the bacte ... | 2005 | 15967186 |
| cytotoxic, antiviral (in-vitro and in-vivo), immunomodulatory activity and influence on mitotic divisions of three taxol derivatives: 10-deacetyl-baccatin iii, methyl (n-benzoyl-(2'r,3's)-3'-phenylisoserinate) and n-benzoyl-(2'r,3's)-3'-phenylisoserine. | the aim of this study was to evaluate cytotoxic, antiviral (in-vitro and in-vivo) and immunomodulatory activity, as well as the influence on mitotic division, of three taxol derivatives representing modified parts of its molecule: 10-deacetyl-baccatin iii, methyl (n-benzoyl-(2'r,3's)-3'-phenylisoserinate) and n-benzoyl(2'r,3's)-3'-phenylisoserine. the cytotoxicity of the compounds, assessed by the formazane method, was relatively low, with a 50% cytotoxic concentration (cc50)>500 microg ml-1. mo ... | 2005 | 15969936 |
| protein transduction of rab9 in niemann-pick c cells reduces cholesterol storage. | niemann-pick disease type c (npc) is a genetic disorder in which patient cells exhibit lysosomal accumulation of cholesterol and sphingolipids (sls) caused by defects in either npc1 or npc2 proteins. we previously demonstrated that npc1 human skin fibroblasts overexpressing endosomal rab proteins (rab7 or rab9) showed a correction in the storage disease phenotype. in the current study, we used protein transduction to further investigate rab9-mediated reduction of stored lipids in npc cells. reco ... | 2005 | 15972801 |
| physical signs for the general dental practitioner. | 2005 | 15977725 | |
| [comparison of adjuvant activities of glucosaminyl-muramyl dipeptide and of the gene coding for granulocyte-macrophage colony-stimulating factor in dna immunization against herpes simplex virus]. | adjuvant activities of granulocyte-macrophage colony-stimulating factor (gm-csf) and synthetic glucosaminyl-muramyl dipeptide (gmdp) were studied in immunization against type 1 herpes simplex virus (hsv1). gene encoding the gd hsv1 protein (pdnagd) was used as an immunogen. gene encoding gm-csf in pdnagm-csf plasmid, which was developed for eukaryotic expression, and gm-dp were used as immune response modulators. gmdp and plasmid dna with inserted gm-csf gene enhanced t-cell immune response to h ... | 2005 | 15981580 |
| a human osteosarcoma cell line expressing herpes simplex type-1 thymidine kinase: studies with radiolabeled (e)-5-(2-iodovinyl)-2'-fluoro-2'-deoxyuridine. | (e)-5-(2-iodovinyl)-2'-fluoro-2'-deoxyuridine (ivfru) is a pyrimidine nucleoside analogue that accumulates selectively in murine cells expressing herpes simplex type-1 thymidine kinase (hsv-1 tk). the uptake of [(125)i]ivfru in human 143b osteosarcoma cells transduced with a retroviral vector bearing the hsv-1 tk gene (143b-ltk cells) is now reported. | 2005 | 15982576 |
| identification of proteins phosphorylated directly by the us3 protein kinase encoded by herpes simplex virus 1. | we have developed a system to analyze the specific protein kinase activity of herpes simplex virus 1 us3 in vitro and shown that us3 directly phosphorylates viral proteins ul34, icp22, and us9 and the cellular protein bad, previously reported to be putative substrates. using this system, we determined the phosphorylation sites of ul34 and identified ul31 as a previously unreported, novel substrate of us3. this system will be useful for further identification of us3 substrates and their phosphory ... | 2005 | 15994828 |
| critical role for the oligoadenylate synthetase/rnase l pathway in response to ifn-beta during acute ocular herpes simplex virus type 1 infection. | we previously demonstrated that ifn-beta transgene treatment protects mouse trigeminal ganglia (tg) cells from acute hsv-1 infection in vitro. however, ifn-alpha6 transgene treatment does not provide protection against acute hsv-1 infection in vitro, even though equivalent levels of ifn are expressed with both transgene treatments. in the present study we show that ifn-beta transgene treatment before acute ocular hsv-1 infection protects mice from hsv-1-mediated mortality, whereas ifn-alpha6 tra ... | 2005 | 16002711 |
| the nectin-1alpha transmembrane domain, but not the cytoplasmic tail, influences cell fusion induced by hsv-1 glycoproteins. | nectin-1 is a receptor for herpes simplex virus (hsv), a member of the immunoglobulin superfamily, and a cellular adhesion molecule. to study domains of nectin-1alpha involved in cell fusion, we measured the ability of nectin-1alpha/nectin-2alpha chimeras, nectin-1alpha/cd4 chimeras, and transmembrane domain and cytoplasmic tail mutants of nectin-1alpha to promote cell fusion induced by hsv-1 glycoproteins. our results demonstrate that only chimeras and mutants containing the entire v-like domai ... | 2005 | 16005040 |
| involvement of il-6 in the paracrine production of vegf in ocular hsv-1 infection. | following ocular hsv-1 infection, neovascularisation of the avascular cornea is a critical event in the pathogenesis of herpetic stromal keratitis. this present study evaluates the role of proinflammatory cytokines such as il-6 in corneal angiogenesis following virus infection. both in vivo and in vitro data indicate that il-6 produced from virus-infected cells can stimulate noninfected resident corneal cells and other inflammatory cells in a paracrine manner to secrete vegf, a potent angiogenic ... | 2006 | 16009363 |
| herpes simplex encephalitis. | 2005 | 16018163 | |
| [elisa study on application of recombinant glycoprotein d of herpes simplex virus-1 in diagnosis of herpes simplex virus-1 infection with elisa]. | to establish a specific and sensitive serological elisa, diagnostic method, for herpes simplex virus-1 (hsv-1) infection using yeast expressed glycoprotein d (gd) of hsv-1 as coating antigen. | 2005 | 16027786 |
| [vp22 enhances the immunological activity of human cytomegalovirus pp65 dna vaccine: experimental study with mice]. | to construct the human cytomegalovirus (hcmv) pp65 dna vaccine vector and vp22 and pp65 coexpressing vector. to evaluate and compare immunological effects in mice. | 2005 | 16029549 |
| high susceptibility of a human oligodendroglial cell line to herpes simplex type 1 infection. | more than 20 infectious agents, ranging from retroviruses to mycobacteria, have been associated with multiple sclerosis onset or relapses in which oligodendrocytes, the myelin-forming cells of the central nervous system, are the initial target of the pathogenic status. in this work, the nature of the susceptibility of the human precursor oligodendroglial kg-1c cell line to herpes simplex virus type 1 (hsv-1) was investigated. infection of kg-1c cells was characterized by a high level of virus pr ... | 2005 | 16036797 |
| targeting proliferating tumor cells via the transcriptional control of therapeutic genes. | we have previously reported the construction of a cell cycle-regulated hsv-1 amplicon vector (denoted as pc8-36) that confers luciferase reporter gene activities dependent on cellular divisions. however, luciferase reporter gene is well known for its relatively high sensitivity, thus, it is crucial to evaluate the therapeutic efficacy of a transcriptional targeted vector. in this report, we have engineered the fasl and fadd genes into pc8-36 and demonstrated their efficacy for the treatment of h ... | 2006 | 16037822 |
| adeno-associated virus-mediated bone morphogenetic protein-7 gene transfer induces c2c12 cell differentiation into osteoblast lineage cells. | to investigate the effects of bone morphogenetic protein-7 (bmp7)-expressing recombinant adeno-associated virus (aav) vector on the differentiation of c2c12 cells. | 2005 | 16038629 |
| epidemiology of genital herpes simplex virus infections in a community-based sample in france: results of the herpimax study. | the objective of this study was to provide information on the prevalence of herpes simplex infections in the general population in europe. | 2005 | 16041253 |
| synthesis of linear, branched, and cyclic peptide chimera. | chimeric peptides are unnatural constructs consisting of bioactive compounds from at least two different peptide(s) and/or protein(s) or two sequences from different parts of the same protein. such multifunctional peptide combinations are prepared to enhance the biological activity or selectivity of their components. new biological effects can also be achieved with the chimera. in this chapter the synthesis of three different types of chimeric peptides will be described. in a linear chimera, two ... | 2005 | 16044540 |
| herpes simplex virus type 2 ul24 gene is a virulence determinant in murine and guinea pig disease models. | a herpes simplex virus type 2 (hsv-2) ul24 beta-glucuronidase (ul24-betagluc) insertion mutant was derived from hsv-2 strain 186 via standard marker transfer techniques. cell monolayers infected with ul24-betagluc yielded cytopathic effect with syncytium formation. ul24-betagluc replicated to wild-type viral titers in three different cell lines. ul24-betagluc was not virulent after intravaginal inoculation of balb/c mice in that all inoculated animals survived doses up to 400 times the 50% letha ... | 2005 | 16051842 |
| herpes keratitis in the absence of anterograde transport of virus from sensory ganglia to the cornea. | herpes stromal keratitis is an immunopathologic disease in the corneal stroma leading to scarring, opacity, and blindness, and it is an important problem in common corneal surgeries. paradoxically, virus antigens are largely focused in the epithelial layer of the cornea and not in the stromal layer, and viral antigens are eliminated before stromal inflammation develops. it is not clear what drives inflammation, whether viral antigens are necessary, or how viral antigens reach the stroma. it has ... | 2005 | 16055558 |
| plasmacytoid dcs help lymph node dcs to induce anti-hsv ctls. | antiviral cell-mediated immunity is initiated by the dendritic cell (dc) network in lymph nodes (lns). plasmacytoid dcs (pdcs) are known to migrate to inflamed lns and produce interferon (ifn)-alpha, but their other roles in antiviral t cell immunity are unclear. we report that ln-recruited pdcs are activated to create local immune fields that generate antiviral cytotoxic t lymphocytes (ctls) in association with lndcs, in a model of cutaneous herpes simplex virus (hsv) infection. although pdcs a ... | 2005 | 16061729 |
| the first 30 minutes in the life of a virus: unrest in the nucleus. | on entry of viral dna into cells, a competition ensues between cellular factors that silence viral genes and transcriptional factors that block silencing and transcribe the dna. in the case of herpes simplex virus 1, the first set of genes expressed after infection-are induced by a viral protein (alphatif or vp16). expression of later (beta,gamma) genes in cells infected at low ratios of virus per cells requires a transcriptional activator (icp4) that cannot block silencing and a protein, icp0, ... | 2005 | 16082207 |
| broad spectrum anti-infective potential of xanthohumol from hop (humulus lupulus l.) in comparison with activities of other hop constituents and xanthohumol metabolites. | this review summarizes the capacity of xanthohumol (xn) in comparison with additional hop constituents and metabolites to act as an antiinfective agent against microorganisms including bacteria, viruses, fungi and malarial protozoa. xn was shown to inhibit the gram-positive bacteria staphylococcus aureus and streptococcus mutans. antiviral activity was demonstrated against bovine viral diarrhea virus, cytomegalovirus, herpes simplex virus type 1 and 2 and human immunodeficiency virus 1. inhibiti ... | 2005 | 16092071 |
| common use of inaccurate antibody assays to identify infection status with herpes simplex virus type 2. | in recent proficiency testing of herpes simplex virus type-specific serologic evidence by the college of american pathologists, commercially available herpes simplex virus antibody assays that were not glycoprotein-g based demonstrated high false-positive rates (14%-88%) for herpes simplex virus type-2 antibodies in sera that were positive for herpes simplex virus type-1 antibodies but negative for herpes simplex virus type-2 antibodies. herpes simplex virus serologic testing should be performed ... | 2005 | 16098855 |
| reduced immune responses after vaccination with a recombinant herpes simplex virus type 1 vector in the presence of antiviral immunity. | due to the continuous need for new vaccines, viral vaccine vectors have become increasingly attractive. in particular, herpes simplex virus type 1 (hsv-1)-based vectors offer many advantages, such as broad cellular tropism, large dna-packaging capacity and the induction of pro-inflammatory responses. however, despite promising results obtained with hsv-1-derived vectors, the question of whether pre-existing virus-specific host immunity affects vaccine efficacy remains controversial. for this rea ... | 2005 | 16099897 |
| characterization of the triplet repeats in the central domain of the gamma134.5 protein of herpes simplex virus 1. | the gamma134.5 protein of herpes simplex virus 1 (hsv-1) consists of an amino-terminal domain, a central domain with triplet repeats (ala-thr-pro) and a carboxyl-terminal domain. the triplet repeats are a unique feature of the gamma134.5 protein encoded by hsv-1, but the number of repeats varies among different strains. notably, the central domain containing the triplet repeats is implicated in neuroinvasion. in this report, it has been shown that partial or full deletion of triplet repeats, i.e ... | 2005 | 16099898 |
| polymerase chain reaction for diagnosis of genital herpes: a missed opportunity? | this study audited the utilization of herpes simplex virus polymerase chain reaction (hsv pcr) in the investigation of recurrent anogenital ulceration at the mortimer market centre. clinic guidelines for use of hsv pcr were modified in april 2003 to expand pcr use. ninety-six case-notes belonging to patients presenting with recurrent anogenital ulceration between 1 april and 16 october 2003 were reviewed and 59 were suitable for inclusion. details of the investigations carried out at each visit ... | 2005 | 16105194 |
| a deubiquitinating enzyme encoded by hsv-1 belongs to a family of cysteine proteases that is conserved across the family herpesviridae. | we have discovered a ubiquitin (ub)-specific cysteine protease encoded within the n-terminal approximately 500 residues of the ul36 gene product, the largest (3164 aa) tegument protein of herpes simplex virus 1 (hsv-1). enzymatic activity of this fragment, ul36usp, is detectable only after cleavage of ul36usp from full-length ul36 and occurs late during viral replication. ul36usp bears no homology to known deubiquitinating enzymes (dubs) or ub binding proteins. sequence alignment of the large te ... | 2005 | 16109378 |
| neonatal herpes should be a reportable disease. | neonatal herpes is a devastating disease, the most serious complication of genital herpes, one of the most common serious congenital or perinatal infections, and the most frequent complication of sexually transmitted infections among children. nevertheless, neonatal herpes is not reportable to health authorities in most states. the potential for prevention has been enhanced by recent diagnostic and therapeutic advances, and the disease meets widely accepted criteria for reporting, including inci ... | 2005 | 16118598 |
| the impact of skin disease following renal transplantation on quality of life. | the immunosuppressive therapy a patient requires to sustain a functioning renal allograft in the long term is associated with various skin complications. while quality of life (qol) after renal transplantation has been studied, no publications document the effect of post-transplant dermatological complications on qol. | 2005 | 16120145 |