Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the introduction of dominant-negative p53 mutants suppresses temperature shift-induced senescence in immortal human fibroblasts expressing a thermolabile sv40 large t antigen. | immortal human fibroblasts, svts8 cells, which express a heat-labile sv40 large t antigen, induces a senescence-like phenomenon in response to upward shift in temperature. cells with arrested division show strong induction of senescence-associated beta-galactosidase. we examined how p53 and prb are involved in this phenomenon since they are major targets of the t antigen. transfection of cells with plasmids encoding the wild-type t antigen or human papilloma virus type 16 e6/e7 proteins complete ... | 1999 | 10050041 |
| human papilloma virus type 16 e6 gene expression in oral exophytic epithelial lesions as detected by in situ rtpcr. | we adopted an in situ reverse transcriptase polymerase chain reaction method of detecting and determining the frequency of early (e6) gene expression of human papilloma virus type 16 at the individual cell level in a sample of oral exophytic lesions with various degrees of epithelial hyperplasia and dysplasia in immunosuppressed and immunocompetent patients. | 1999 | 10052376 |
| risk factors for abnormal anal cytology in young heterosexual women. | although anal cancers are up to four times more common in women than men, little is known about the natural history of anal human papillomavirus (hpv) infections and hpv-related anal lesions in women. this study reports on the prevalence of and risks for anal cytological abnormalities over a 1-year period in a cohort of young women participating in a study of the natural history of cervical hpv infection. in addition to their regularly scheduled sexual behavior interviews and cervical testing, c ... | 1999 | 10067816 |
| immune responses against human papillomavirus (hpv) type 16 virus-like particles in a cohort study of women with cervical intraepithelial neoplasia. ii. systemic but not local iga responses correlate with clearance of hpv-16. | to investigate whether there is an association between local or systemic igg and iga responses against human papillomavirus (hpv) type 16 virus-like particles (vlp) containing l1 and l2 and the possible influence of these responses on clearance of hpv-16 and its associated lesions, cervical mucus samples from 125 patients and plasma samples from 100 patients, all participating in a non-intervention cohort study of women with abnormal cytology, were analysed. the results show that local igg and i ... | 1999 | 10073701 |
| effects of yokuinin on the therapeutic efficacy of a new benzoxazinorifamycin krm-1648 against mycobacterium avium infection. | the chinese traditional medicine, yokuinin, which has anti-inflammatory effects and anti-human papilloma virus activity, was examined for its effects on the therapeutic efficacy of a benzoxazinorifamycin krm-1648 (krm) against myobacterium avium infection in mice. reverse transcription-pcr analysis revealed that yokuinin increased the mrna expression of all test cytokines in lung tissues of infected ice at week 8, in the order transforming growth factor-beta (tgf-beta) > ifn-gamma > tnf-alpha > ... | 1999 | 10075281 |
| kinetic selection of hpv 16 e6/e7-directed antisense nucleic acids: anti-proliferative effects on hpv 16-transformed cells. | the e6/e7-coding sequences of the human papillomavirus type 16 (hpv 16) were probed for kinetic accessibility in vitro by pools of catalytic antisense rna. only long-chain complementary rna and very few antisense sequences with a 3' portion complementary to a 10 nt window within unspliced and spliced e6-coding target sequences showed fast annealing with k(ass) values of up to 10(4) m-1s-1 indicating that the majority of e6/e7 rna sequences are unfavourable targets for antisense inhibitors and ri ... | 1999 | 10075988 |
| induction of tumor antigen-specific immunity using plasmid dna immunization in mice. | we have evaluated the ability of bioballistic "gene gun" immunization of mice with plasmid dna encoding clinically relevant tumor antigens to induce protective antitumor immunity. mice immunized with plasmid cdna encoding the cervical carcinoma-associated human papillomavirus 16-e7 gene product exhibited potent anti-e7-specific cytotoxic t lymphocytes and were protected completely against a subsequent challenge with the e7+ c3 sarcoma. of perhaps greater clinical interest, genetic immunization u ... | 1999 | 10078966 |
| colonization of in vitro-formed cervical human papillomavirus- associated (pre)neoplastic lesions with dendritic cells: role of granulocyte/macrophage colony-stimulating factor. | the purpose of this study was to investigate the ability of cd1a+ langerhans/dendritic cells (lcs/dcs) to infiltrate human papillomavirus (hpv)-associated (pre)neoplastic lesions of the uterine cervix. migration of lcs/dcs in the presence of keratinocytes derived from normal cervix and hpv-transformed cell lines was evaluated in boyden chambers and in organotypic cultures and correlated with granulocyte/macrophage colony-stimulating factor (gm-csf) production by the cells, as determined by elisa ... | 1999 | 10079255 |
| synergism with germ line transcription factor oct-4: viral oncoproteins share the ability to mimic a stem cell-specific activity. | activation of transcription by oct-4 from remote binding sites requires a cofactor that is restricted to embryonal stem cells. the adenovirus e1a protein can mimic the activity of this stem cell-specific factor and stimulates oct-4 activity in differentiated cells. here we characterize the oct-4-e1a interaction and show that the e1a 289r protein harbors two independent oct-4 binding sites, both of which specifically interact with the pou domain of oct-4. furthermore, we demonstrate that, like e1 ... | 1999 | 10082529 |
| control of replicative life span in human cells: barriers to clonal expansion intermediate between m1 senescence and m2 crisis. | the accumulation of genetic abnormalities in a developing tumor is driven, at least in part, by the need to overcome inherent restraints on the replicative life span of human cells, two of which-senescence (m1) and crisis (m2)-have been well characterized. here we describe additional barriers to clonal expansion (mint) intermediate between m1 and m2, revealed by abrogation of tumor-suppressor gene (tsg) pathways by individual human papillomavirus type 16 (hpv16) proteins. in human fibroblasts, a ... | 1999 | 10082577 |
| [virologic aspects of juvenile laryngeal papillomatosis]. | juvenile laryngeal papillomatosis is the most common benign tumor of the larynx in childhood. the specific etiological factors are non-oncogenic human papillomavirus types 6 and 11. in the present study two cases (a 6-year-old male and a 5 and a half-year-old female) operated five times each and harbouring type 11 dna in papillomas excised in the first operations are analysed from the following virological aspects: 1. the examination of vertical transmission by general primer-polymerase chain re ... | 1999 | 10083813 |
| overexpression of d-type cyclins, e2f-1, sv40 large t antigen and hpv16 e7 rescue cell cycle arrest of tsbn462 cells caused by the ccg1/taf(ii)250 mutation. | tsbn462 cells, which have a point mutation in ccg1/taf(ii)250, a component of tfiid complex, arrest in g1 at the nonpermissive temperature of 39.5 degrees c. overexpression of d-type cyclins rescued the cell cycle arrest of tsbn462 cells, suggesting that the cell cycle arrest was through rb. consistent with this, overexpression of e2f-1, whose function is repressed by the hypophosphorylated form of rb, also rescued the cell cycle arrest. moreover, expression of the viral oncoproteins sv40 large ... | 1999 | 10086334 |
| cumulation of tp53 mutations and p16ink4a/p15ink4b homozygous deletions in human papilloma virus type 16 positive scrotal cancer. | scrotal cancer is the first described occupational cancer. the frequency of occupation-related scrotal cancer is very rare because of better hygiene and protective clothing. human papilloma viruses (oncogenic types 16 and 18) were reported as the causative agents in the pathogenesis of scrotal cancers. e5, e6, and e7 proteins, expressed by human papilloma virus type 16, affect the cell cycle at the g1 checkpoint. tp53, p16ink4a, and p15ink4b were reported as the transcription factors that regula ... | 1999 | 10087941 |
| comparison of in-situ hybridization, direct and indirect in-situ pcr as well as tyramide signal amplification for the detection of hpv. | one hundred paraffin-embedded cervical biopsy specimens were tested for the presence of human papilloma virus (hpv) by in situ hybridization (ish), and by direct and indirect in situ pcr (is-pcr) in order to evaluate the efficiency of the different in situ methods in detecting hpv infection. ish was performed using either commercial dna probes or a cocktail of 5'-digoxigenin labeled oligoprimers. the same were used for ish during indirect is-pcr. to enhance the sensitivity of ish several polymer ... | 1999 | 10090569 |
| [natural course of hpv infection. usefulness of hpv analysis in cervix diagnosis]. | cervical carcinomas and their precursors (cervical dysplasia, cin1-3) are associated with human papillomavirus (hpv) infections. epidemiological and in vitro-studies have shown that some of the genital hpv types, the high risk-types 16, 18, 31 etc., code for proteins (e6/e7) which strongly influence the cell cycle and genome stability. progression from weak to severe dysplasia and to invasive cancer is associated with increasing expression of these viral oncogenes. which additional cofactors con ... | 1999 | 10091228 |
| viruses and human cancer. | several kinds of viruses cause cancer in humans, accounting for 10-20% of cancer worldwide. human cancer viruses include human papillomavirus, hepatitis b virus, epstein-barr virus, human t cell lymphotropic virus, and kaposi's sarcoma-associated herpesvirus. cancer viruses alter the machinery of the infected cell to promote their own survival and growth. in the process, they interfere with normal cellular control mechanisms, leading to abnormal growth, genetic alterations and malignancy. studie ... | 1998 | 10093516 |
| expression of the e6 and e7 genes of human papillomavirus (hpv16) extends the life span of human myoblasts. | primary human myoblasts (satellite cells), like other human cells, have a limited life span in vitro. here we show that expression of the e6e7 early region from human papillomavirus type 16 can greatly extend the life span of both fetal and satellite cell-derived myoblasts and release them from dependence on the growth factors normally necessary for their proliferation. expression of either the e6 or the e7 gene alone was not sufficient to confer this phenotype, although expression of e7 did del ... | 1999 | 10094825 |
| human papillomavirus testing in the clinical laboratory. part ii: vaginal, vulvar, perineal, and penile squamous lesions. | the accurate diagnosis of hpv-related diseases of the lower genital tract requires expertise, and sometimes even the expert may face a dilemma as to the precise nature of the biopsies submitted from colposcopically suspicious hpv-related lesions. we have evaluated the role of viral testing using dot blot hybridization (viratype) and pcr in the diagnosis of histologically typical (42 cases) and equivocal (30 cases) squamous intraepithelial lesions of the vagina (7), vulva (30), perianal epitheli ... | 1993 | 10150115 |
| dna replication specificity and functional e2 interaction of the e1 proteins of human papillomavirus types 1a and 18 are determined by their carboxyl-terminal halves. | replication of most papillomaviruses (pvs) requires the viral-encoded e1 and e2 proteins that bind to the origin of replication (ori) containing the e1- and e2-binding sites and help recruit host replication factors during the initiation of dna replication. we studied the ability of heterologous e1 and e2 proteins to interact in vivo and support replication, using the human papillomavirus (hpv) types 1a and 18 as model systems. the e1 protein of hpv-1a in combination with hpv-18 e2 supported hig ... | 1999 | 10191198 |
| ccaat displacement protein binds to and negatively regulates human papillomavirus type 6 e6, e7, and e1 promoters. | expression of human papillomavirus genes increases as the target cell, the keratinocyte, differentiates. ccaat displacement protein (cdp) is a cellular protein which has been shown in other cell types to negatively regulate gene expression in undifferentiated cells but not in differentiated cells. we have previously shown that a 66-bp purine-thymidine-rich sequence (the 66-mer) binds cdp and negatively regulates the human papillomavirus type 6 (hpv-6) e6 promoter (s. pattison, d. g. skalnik, and ... | 1999 | 10196318 |
| an aggressive and invasive growth of juvenile papillomas involving the total respiratory tract. | a malignant course of juvenile laryngeal papillomatosis has rarely been reported. in the present case the patient had had laryngeal papillomas since the age of three years. the papillomas gradually spread to the entire respiratory system, and during 30 years the patient was operated on more than 80 times. at present an invasive tumour spreading from the tongue into the parapharyngeal space, extending to the cranial base, has been demonstrated by magnetic resonance imaging (mri). intralesional th ... | 1998 | 10197157 |
| biological behavior and etiology of inflammatory cervical smears. | two hundred and fifty-seven consecutive women attending a major maternal and child health (mch) center were studied clinically, colposcopically, cytologically, and microbiologically for different gynecologic infections. out of 257 cases, 207 (80.5%) had inflammatory cervical smears, of which 183 (88.4%) were infected with one or more genital tract infections. bacterial vaginosis (risk, 22.6-fold), chlamydia (risk, 21.6-fold), and human papillomavirus (hpv) (risk, 13.5-fold) were independently as ... | 1999 | 10204101 |
| a human papillomavirus e2 transcriptional activator. the interactions with cellular splicing factors and potential function in pre-mrna processing. | the human papillomavirus (hpv) e2 protein plays an important role in transcriptional regulation of viral genes as well as in viral dna replication. unlike most types of hpv, the e2 protein of epidermodysplasia verruciformis (ev)-associated hpvs harbors a relatively long hinged region between the terminal, conserved transactivation and dna binding/dimerization domains. the sequence of ev-hpv e2 hinge contains multiple arginine/serine (rs) dipeptide repeats which are characteristic of a family of ... | 1999 | 10207001 |
| a two-tier polymerase chain reaction direct sequencing method for detecting and typing human papillomaviruses in pathological specimens. | an in-house polymerase chain reaction direct sequencing (pcr-ds) approach for hpv detection and typing was developed, taking advantage of two widely used pairs of human papillomavirus (hpv)-specific pcr primers, my09/my11 and gp5/gp6, and 33p-labeled dideoxynucleotides. in this study, 105 pathological specimens were examined: 89% were diagnosed as cervical intraepithelial neoplasia (cin) grade i-iii, 76.2% were hpv-positive by pcr-ds. the pcr using gp5/gp6 (first tier) and my09/my11 primers (sec ... | 1998 | 10207670 |
| an unusual cervical carcinoma showing exception to epitheliotropism of human papillomavirus. | human papillomaviruses (hpv) infect epithelial tissues but have not been previously detected within mesenchymal cells. during a systematic investigation of figo stage ib cervical cancers with colorimetric in situ hybridization, we detected hpv 16 dna within the stromal compartment of an unusual undifferentiated carcinoma. the mesenchymal nature of the hpv-containing cells was confirmed by immunohistochemistry and electron microscopy. no viral particles were identified. sequencing the majority of ... | 1999 | 10208473 |
| the antigen-presenting environment in normal and human papillomavirus (hpv)-related premalignant cervical epithelium. | the activation of hpv-specific t cells within the cervical microenvironment is likely to play an important part in the natural history of cervical intraepithelial neoplasia (cin). the extent and the type of t cell activation will depend critically on the expression of mhc, costimulatory cell surface molecules and cytokines by keratinocytes and langerhans cells within the cervical lesion. expression of mhc class ii (hla-a-dr and -dq), costimulatory/adhesion molecules (cd11a/18, cd50, cd54, cd58 a ... | 1999 | 10209502 |
| smoking and oral contraceptives as risk factors for cervical carcinoma in situ. | human papillomavirus (hpv) is probably a necessary but definitely not a sufficient cause of cervical carcinoma. however, it remains unclear which factors, in addition to hpv, are important for the development of cervical carcinoma and its precursor lesions. to address this issue, we conducted a case-control study nested in a population-based cohort consisting of women participating in cytological screening in one swedish county, any time during 1969 through 1995. detailed information on sexual p ... | 1999 | 10209949 |
| interleukin-12-secreting human papillomavirus type 16-transformed cells provide a potent cancer vaccine that generates e7-directed immunity. | the development of a vaccine that would be capable of preventing or curing the (pre)cancerous lesions induced by genital oncogenic human papillomaviruses (hpvs) is the focus of much research. many studies are presently evaluating vaccines based on the viral e6 and e7 oncoproteins, both of which are continually expressed by tumor cells. the success of a cancer vaccine relies, in large part, on the induction of a tumor-specific th1-type immunity. in this study, we have evaluated the ability of b7- ... | 1999 | 10209958 |
| expression patterns of the human papillomavirus type 16 transcription factor e2 in low- and high-grade cervical intraepithelial neoplasia. | specific antibodies against the c-terminus of e2, produced by affinity purification of polyclonal antisera, have been used to identify the cellular populations which express the hpv 16 e2 transcription factor, in a series of formalin-fixed, paraffin-embedded cervical tissues. cases were selected for both the presence of hpv 16 dna (confirmed by multiple gene-specific pcr detections) and the presence of multiple grades of cervical intraepithelial neoplasia (cin). the data indicate that e2 express ... | 1998 | 10211116 |
| human papillomavirus (hpv) in high-grade cervical intraepithelial neoplasia (cin) detected by morphology and polymerase chain reaction (pcr)-- a cytohistologic correlation of 277 cases treated by laser conization. | the aims of this study were to evaluate the cytohistologic correlation in women treated for high-grade lesions of the cervix uteri (hg cin), to assess the distribution of hpv features and finally to test the validity of the morphological criteria of hpv infection. the smears and biopsy specimens from 277 women treated for hg cin by laser conization were re-evaluated blindly. tissue blocks (n = 188) and 52 archival smears were examined for hpv dna using pcr. hpv changes were detected with equal f ... | 1999 | 10211617 |
| intratype sequence variation among clinical isolates of the human papillomavirus type 6 l1 orf: clustering of mutations and identification of a frequent amino acid sequence variant. | human papillomavirus type 6 (hpv-6) is the causative agent of condyloma acuminata, a common sexually transmitted disease. virus-like particles (vlps) assembled from the l1 major capsid protein represent promising candidates for prophylactic vaccines. however, any intratype sequence variation among hpv-6 l1 orfs will influence which sequence is used for a vaccine according to its prevalence in the population and its propensity for vlp production. therefore, we have analysed the entire l1 nucleoti ... | 1999 | 10211973 |
| tobacco smoking as a risk factor in anal carcinoma: an antiestrogenic mechanism? | human papillomavirus-associated anogenital carcinogenesis depends on poorly defined cofactors. smoking was recently suggested to increase the risk of anal cancer more in premenopausal women than in postmenopausal women. thus, we used our population-based anal cancer case-control study in denmark and sweden to test this hypothesis. | 1999 | 10218509 |
| [study of human papillomavirus via chemiluminescence technic and polymerase chain reaction in transitional cell carcinoma of the bladder]. | the exact ethiology of bladder carcinoma isn't yet known; an implication of human papillomavirus (hpv) has been recently hypothesized. | 1998 | 10220998 |
| human papillomavirus and prognoses of patients with cancers of the upper aerodigestive tract. | some studies have shown that human papillomavirus (hpv) infection may play not only an etiologic role in anogenital cancers but also a role in the clinical outcome. the objective of the current study was to determine whether detection of hpv dna in primary squamous cell carcinomas of the upper aerodigestive tract (uadt) is a prognostic factor in patients with the disease. | 1999 | 10223228 |
| interlaboratory agreement of different human papillomavirus dna detection and typing assays in cervical scrapes. | 1999 | 10225460 | |
| prevalence of human papilloma virus dna in head and neck cancers carrying wild-type or mutant p53 tumor suppressor genes. | the normal function of the p53 tumor suppressor protein can be perturbed by non-mutational mechanisms. the e6 protein encoded by high risk strains of human papilloma virus (hpv) targets the p53 protein resulting in enhanced degradation via the ubiquitin pathway. we have used nested pcr for detecting the presence of hpv dna in 58 primary head and neck tumors and 15 metastatic lymph nodes, which had been prescreened for p53 mutations in exons 5 to 8. hpv dna sequences were detected in 12 tumors (2 ... | 1999 | 10226517 |
| chimeric papillomavirus virus-like particles induce a murine self-antigen-specific protective and therapeutic antitumor immune response. | the use of chimeric virus-like particles represents a new strategy for delivering tumor antigens to the immune system for the initiation of antitumor immune responses. immunization of dba/2 mice with the p1a peptide derived from the p815 tumor-associated antigen p1a induced specific t-cell tolerance, resulting in progression of a regressor p815 cell line in all animals. however, immunization with a human papillomavirus type 16 l1 virus-like particle containing the p1a peptide in the absence of a ... | 1999 | 10227378 |
| human papillomavirus types 16 and 39 in a vulval carcinoma occurring in a woman with hailey-hailey disease. | a woman with hailey-hailey disease, suffering from carcinoma of the vulva, was examined by histology and for the presence of human papillomavirus (hpv) dna by polymerase chain reaction (pcr) and in situ hybridization. our diagnosis by histological examination revealed the vulval carcinoma to be a squamous cell carcinoma (scc), adjacent to lesions of hailey-hailey disease and severe dysplasia/carcinoma in situ [vulval intraepithelial neoplasia (vin) iii]. the pcr with consensus primers for the l1 ... | 1999 | 10233276 |
| allelic loss in human papillomavirus-positive and -negative vulvar squamous cell carcinomas. | vulvar squamous cell carcinoma (vscc) is a biologically and morphologically diverse disease, consisting of human papillomavirus (hpv)-positive and -negative tumors that differ in their morphological phenotypes and associated vulvar mucosal disorders. this study analyzed the frequencies of allelic loss (loss of heterozygosity (loh)) in hpv-positive and -negative vsccs to identify potential targets for the study of preinvasive diseases, to determine whether hpv status influenced patterns of loh, a ... | 1999 | 10233839 |
| characterization of a major neutralizing epitope on human papillomavirus type 16 l1. | persistent infection with human papillomavirus type 16 (hpv-16) is strongly associated with the development of cervical cancer. neutralizing epitopes present on the major coat protein, l1, have not been well characterized, although three neutralizing monoclonal antibodies (mabs) had been identified by using hpv-16 pseudovirions (r. b. roden et al., j. virol. 71:6247-6252, 1997). here, two of these mabs (h16.v5 and h16.e70) were demonstrated to neutralize authentic hpv-16 in vitro, while the thir ... | 1999 | 10233949 |
| trichostatin a up-regulates human papillomavirus type 11 upstream regulatory region-e6 promoter activity in undifferentiated primary human keratinocytes. | human papillomavirus (hpv) gene expression in squamous epithelia is differentiation dependent in benign patient lesions and in organotypic raft cultures of primary human keratinocytes (phks). using the lacz reporter in raft cultures, we previously showed that this transcriptional regulation of the hpv type 11 (hpv-11) enhancer-promoter located in the upstream regulatory region (urr) appears to have resulted from coordination between the transcription transactivators ap1, oct1, and sp1 in differe ... | 1999 | 10233965 |
| identifying women with cervical neoplasia: using human papillomavirus dna testing for equivocal papanicolaou results. | a papanicolaou (pap) test result of atypical squamous cells of undetermined significance (ascus) presents a clinical challenge. only 5% to 10% of women with ascus harbor serious cervical disease, but more than one third of the high-grade squamous intraepithelial lesions (hsils) in screening populations are identified from ascus pap test results. | 1999 | 10235153 |
| resolution of recalcitrant hand warts in an hiv-infected patient treated with potent antiretroviral therapy. | human papilloma virus (hpv)-related cutaneous manifestations occur with increased frequency and severity among hiv-infected persons. in this report, we describe an hiv-infected man with persistent, severe cutaneous hand warts that did not respond to multiple therapies, including liquid nitrogen cryotherapy, topical dinitrochlorobenzene, topical podophyllin, and intralesional interferon-alfa injections. approximately 1 year after starting a potent protease inhibitor-containing antiretroviral regi ... | 1999 | 10321624 |
| induction of the p53-target gene gadd45 in hpv-positive cancer cells. | the e6 oncoprotein of human papillomaviruses (hpvs) has the potential to functionally antagonize p53. in several experimental model systems, ectopic expression of e6 can block the genotoxic induction of the growth inhibitory p53 target gene gadd45, suggesting that the inactivation of this pathway may play a major role for hpv-associated cell transformation. here, we investigated whether this reflects the regulation of gadd45 expression in carcinoma-derived hpv-positive cells. we found that the g ... | 1999 | 10327059 |
| is human papillomavirus testing of value in clinical practice? | the objective of this review was to evaluate the current status of human papillomavirus testing in predicting the presence of high-grade or invasive disease in the cervix in women with papanicolaou smears reported as atypical squamous cells of undetermined significance (ascus) or low-grade squamous intraepithelial lesion. this is a review of many of the studies already published evaluating the utility of human papillomavirus testing as part of the triage for patients whose papanicolaou smears we ... | 1999 | 10329854 |
| cervical human papillomavirus deoxyribonucleic acid persists throughout pregnancy and decreases in the postpartum period. | our goal was to determine the persistence of human papillomavirus infection of the cervix in a prospectively evaluated cohort of pregnant women observed from the first trimester until after delivery. | 1999 | 10329863 |
| target site selection for an rna-cleaving catalytic dna. | a small catalytic dna, known as the 10-23 dna enzyme or deoxyribozyme, has been shown to efficiently hydrolyze rna at purine-pyrimidine (r-y) junctions in vitro. although these potentially cleavable junctions are ubiquitous, they are often protected from deoxyribozyme activity by rna secondary structure. we have developed a multiplex cleavage assay for screening the entire length of a target rna molecule for deoxyribozyme cleavage sites that are efficient, both in terms of kinetics and accessibi ... | 1999 | 10331809 |
| hpv in situ hybridization with catalyzed signal amplification and polymerase chain reaction in establishing cerebellar metastasis of a cervical carcinoma. | we report an unusual case of cerebellar metastasis from a cervical adenosquamous carcinoma in which molecular techniques assisted in establishing the correct diagnosis. the patient was a 43-year-old woman with surgically unresectable cervical carcinoma diagnosed 2 years before presenting with neurological symptoms. a magnetic resonance imaging scan showed a large, enhancing cerebellar lesion with significant brain stem compression. the excised cerebellar tumor resembled a small cell carcinoma an ... | 1999 | 10333232 |
| thin-layer cytology meets viral hybrid capture. | this commentary addresses the benefits of liquid-based thin-layer cervical cytology, and the applicability of residual samples for human papilloma virus assay. the potential for use of this technology in wide-scale screening as well as the many unanswered issues, in particular cost-benefit ratio and financing, are also discussed. | 1999 | 10342014 |
| the proliferative properties of tumor cells differentially correlate with the host immune responses in anogenital bowen's disease. | bowen's disease is a squamous cell carcinoma in situ that rarely invades into the underlying dermis. in order to evaluate the relationship between the cytological properties of the tumor cells and the host immune response, we have examined the expression of p53 and proliferating cell nuclear antigen (pcna), and the number of mitotic cells, clumping cells, koilocytes, langerhans cells (lcs) and dermal lymphoid cell infiltration in 18 cases of anogenital bowen's disease. when compared with normal ... | 1998 | 10342748 |
| hpv testing in the evaluation of the minimally abnormal papanicolaou smear. | minor cytologic abnormalities of the cervix, such as atypical squamous cells of undetermined significance (ascus), are vastly more common than high-grade squamous intraepithelial lesions or invasive cancer. current guidelines for the management of ascus include repeating the papanicolaou (pap) smear at specific intervals, referring all patients for colposcopy or using an adjunctive test such as hybrid capture human papillomavirus (hpv) testing or cervicography. the usefulness of the pap smear is ... | 1999 | 10348071 |
| [the application of the polymerase chain reaction technic for the detection of human papillomavirus sequences]. | the polymerase chain reaction technique was applied to detect sequences of human papillomavirus (hpv) by controls of cellular lines of cervical cancer and of tissues obtained through biopsy with a hpv-positive clinical diagnosis. a set of consensus oligonucleotides, which are complementary to a highly conserved region within the open reading frame e1 of the viral genome of hpv affecting the cervical mucosa, was used. with these primers it was possible to amplify dna sequences corresponding to hp ... | 1998 | 10349442 |
| comèl-netherton syndrome complicated by papillomatous skin lesions containing human papillomaviruses 51 and 52 and plane warts containing human papillomavirus 16. | we describe a 28-year-old woman with characteristic clinical signs of comèl-netherton syndrome (cns) who showed numerous plane warts on her face and forearms and papillomatous skin tumours affecting her groins and genitoanal skin. using human papillomavirus (hpv) type-specific primers for cutaneous and mucosal hpv types we identified hpv 16-specific sequences in plane warts and hpv 51- and hpv 52-specific dna in papillomatous skin from the patient's groins, suggesting a pathogenetic role (cofact ... | 1999 | 10354085 |
| induction of human papillomavirus-specific cd4(+) and cd8(+) lymphocytes by e7-pulsed autologous dendritic cells in patients with human papillomavirus type 16- and 18-positive cervical cancer. | human papillomavirus (hpv) type 16 (hpv 16) and hpv type 18 (hpv 18) are implicated in the induction and progression of the majority of cervical cancers. since the e6 and e7 oncoproteins of these viruses are expressed in these lesions, such proteins might be potential tumor-specific targets for immunotherapy. in this report, we demonstrate that recombinant, full-length e7-pulsed autologous dendritic cells (dc) can elicit a specific cd8(+) cytotoxic t-lymphocyte (ctl) response against autologous ... | 1999 | 10364287 |
| the human papillomavirus type 16 e6 gene alone is sufficient to induce carcinomas in transgenic animals. | high-risk human papillomaviruses (hpvs) are the causative agents of certain human cancers. hpv type 16 (hpv16) is the papillomavirus most frequently associated with cervical cancer in women. the e6 and e7 genes of hpv are expressed in cells derived from these cancers and can transform cells in tissue culture. animal experiments have demonstrated that e6 and e7 together cause tumors. we showed previously that e6 and e7 together or e7 alone could induce skin tumors in mice when these genes were ex ... | 1999 | 10364340 |
| detection of human papillomavirus types 6 and 11 in pubic and perianal hair from patients with genital warts. | genital human papillomavirus (hpv) types 6 and 11 are of clinical importance due to their role in the development of anogenital warts. a pilot study was performed to investigate whether dnas from hpv types 6 and 11 are present in hairs plucked from the pubic and perianal regions and eyebrows of patients with genital warts at present and patients with a recent history of genital warts. genital hpv dna was detected in 9 of 25 (36%) pubic hair samples and in 11 of 22 (50%) perianal hair samples by ... | 1999 | 10364596 |
| human papilloma virus dna detection in oral lesions in the greek population. | hpvs (human papilloma viruses) are small dna "epitheliotropic" viruses, implicated in cervical carcinogenesis, particularly the "high-oncogenic-risk" types hpv-16 and hpv-18. data concerning oral carcinogenesis are however, contradictory. we examined the presence of hpv and subsequently hpv-16 and hpv-18 in 102 specimens of paraffin-embedded oral tissue blocks--81 oral squamous cell carcinomas and 21 oral hyperplasias--using pcr technique followed by agarose gel electrophoresis. our results demo ... | 1999 | 10365111 |
| response to il-6 of hpv-18 cervical carcinoma cell lines. | the human papillomavirus type 18 (hpv-18) upstream regulatory region (urr) controls cell type-specific expression of the viral oncoproteins e6 and e7. the hpv-18 urr is active in the cervical carcinoma cell line hela but inactive in the hepatoma cell line hepg2. c/ebpss (nf-il-6) was shown to participate as an important regulator in hpv transcription dependent on the cell type. the finding that c/epbss is critical for hpv-18 urr activity and that c/epbss is induced by il-6 offers the opportunity ... | 1999 | 10366571 |
| oral vaccination of mice with human papillomavirus virus-like particles induces systemic virus-neutralizing antibodies. | to assess whether oral vaccination against human papillomavirus (hpv) may be feasible, we administered hpv virus-like particles (vlps) to mice by gavage. enzyme-linked immunosorbent assay (elisa) results indicated that serum anti-vlp immunoglobulin g (igg) and iga antibodies were induced after oral vaccination, and these responses demonstrated antigenic specificities that were conformationally dependent and restricted according to hpv genotype. importantly, orally induced postimmune sera were fo ... | 1999 | 10367945 |
| pap smears: screening, interpretation, treatment. | cervical cancer remains an important public health problem in the united states. cervical dysplasia and rarely carcinoma in situ can occur during adolescence, especially in adolescents who become sexually active with multiple partners or who have been exposed to hpv, especially types 16 and 18. papanicolaou smear is recommended for any adolescent who has ever been sexually active or exposed to human papillomavirus or is 18 years of age. this article discusses the new classification systems for c ... | 1999 | 10370708 |
| advances in the diagnosis and treatment of human papillomavirus infections. | during the past decade, much has been learned about the natural history of hpv. most infections occur early in one's sexual life. the overwhelming majority of infections are cleared by the host immune system and never present as warts or neoplasia. certain patient behavior such as smoking, frequent sex with many different partners, other std infections, especially hiv, and immune-suppressive drugs promote hpv expression and cause persistent infection. persistent hpv infection is very strongly re ... | 1999 | 10370842 |
| a matched prospective study of human immunodeficiency virus serostatus, human papillomavirus dna, and cervical lesions detected by cytology and colposcopy. | to compare the prevalence and type of human papillomavirus (hpv) infections in the genital tract of human-immunodeficiency-virus- (hiv) seropositive and -seronegative women matched for cytology and to examine prospectively the relationship of hpv dna, colposcopic findings and cervical squamous intraepithelial lesions (sil) in these matched seropositive and seronegative cohorts. | 1999 | 10371475 |
| activation of rna polymerase iii transcription in cells transformed by simian virus 40. | rna polymerase (pol) iii transcription is abnormally active in fibroblasts that have been transformed by simian virus 40 (sv40). this report presents evidence that two separate components of the general pol iii transcription apparatus, tfiiib and tfiiic2, are deregulated following sv40 transformation. tfiiic2 subunits are expressed at abnormally high levels in sv40-transformed cells, an effect which is observed at both protein and mrna levels. in untransformed fibroblasts, tfiiib is subject to r ... | 1999 | 10373542 |
| detection of human papilloma virus and epstein-barr virus dna in nasopharyngeal carcinoma by polymerase chain reaction. | nasopharyngeal carcinoma (npc) is an unique epithelial malignancy which occurs at a high frequency in certain regions of southeast asia. human papilloma virus (hpv) and epstein-barr virus (ebv) have both been identified in tissue specimens from npc. nevertheless, the association between viral infection and npc remains unclear. the purpose of this study was to demonstrate that simultaneous infection with ebv and hpv can occur in npc. eighty-eight fresh tissue samples which contained sufficient an ... | 1999 | 10375867 |
| evaluation of the risk of cervical intraepithelial neoplasia and human papilloma virus infection in renal transplant patients receiving immunosuppressive therapy. | to investigate the risk of cervical intraepithelial neoplasia and the coexistence of human papilloma virus (hpv) infection in renal transplant patients receiving immunosuppressive therapy. | 1999 | 10376431 |
| the nature of the attenuation of salmonella typhimurium strains expressing human papillomavirus type 16 virus-like particles determines the systemic and mucosal antibody responses in nasally immunized mice. | we have recently shown by using a recombinant salmonella typhimurium phopc strain in mice the feasibility of using a salmonella-based vaccine to prevent infection by the genital human papillomavirus type 16 (hpv16). here, we compare the hpv16-specific antibody responses elicited by nasal immunization with recombinant s. typhimurium strains harboring attenuations that, in contrast to phopc, are suitable for human use. for this purpose, chi4989 (deltacya deltacrp) and chi4990 [deltacya delta(crp-c ... | 1999 | 10377159 |
| [transformation of immortalized rat embryonic fibroblasts by the e7 gene of human type 16 papillomavirus]. | 1999 | 10377575 | |
| rapid dna mutation identification and fingerprinting using base excision sequence scanning. | base excision sequence scanning (bess) is a new polymerase chain reaction (pcr)-based mutation scanning method that locates and identifies all dna mutations. the bess method consists of two procedures that generate "t" (bess t-scan) and "g" ladders (bess g-tracker) analogous to t and g ladders of dideoxy sequencing. the bess procedures are simple to perform and require no special equipment or gels, no reaction optimization beyond pcr, and no heteroduplex formation. the samples are analyzed on st ... | 1999 | 10380756 |
| implication of p53 in growth arrest and apoptosis induced by the synthetic retinoid cd437 in human lung cancer cells. | cd437 is a novel retinoid that can induce apoptosis in a variety of tumor cell types by an unknown mechanism. we found that cd437 up-regulated the expression of p21(waf1/cip1), bax, and killer/dr5 and induced g1 arrest and rapid apoptosis in three human non-small cell lung carcinoma cell lines with wild-type p53 but not in five cell lines with mutant p53, suggesting a role for p53 in the effects of cd437. using h460 cells in which wild-type p53 protein was degraded by transfection of the human p ... | 1999 | 10383141 |
| p16/prb pathway alterations are required for bypassing senescence in human prostate epithelial cells. | the cell cycle regulatory genes p16/cdkn2 and rb are frequently deleted in prostate cancers. in this study, we examined the role of alterations in p16 and prb during growth, senescence, and immortalization in vitro of human prostate epithelial cells (hpecs). hpecs are established from normal prostate tissues and cultured on collagen-coated dishes. our results show that p16 is reproducibly elevated at senescence in hpecs. hpecs are immortalized using human papilloma virus 16 e6 and/or e7 as molec ... | 1999 | 10383161 |
| the human papillomavirus type 11 upstream regulatory region triggers hair-follicle-specific gene expression in transgenic mice. | we have generated transgenic mice carrying the urr of the human papillomavirus type 11 ligated in front of the escherichia coli beta-galactosidase coding region sequence. using x-gal staining to demonstrate beta-galactosidase production, we observed a hair-specific transcription of the reporter gene. this transcription was limited to the epithelial cells of the hair bulge region. the transgene was developmentally regulated, as no lacz staining was demonstrated during embryogenesis and specific s ... | 1999 | 10383735 |
| squamous cell carcinoma of the vulva in brazil: prognostic importance of host and viral variables. | certain clinicopathologic features of vulvar squamous cell carcinoma have been correlated with adverse prognosis. however, few large-scale studies have addressed their role in patient survival. this study examined the relationship between multiple variables and prognosis in a large group of vulvar cancers in brazil. | 1999 | 10385552 |
| anogenital human papilloma virus and the problem of persistence. | it has been known since antiquity that genital warts (condylomata acuminata) are a sexually transmitted disease. since the late 1970s it has become increasingly clear that infections by the same virus, human papilloma virus (hpv) are closely implicated in the aetiology of anogenital squamous cell carcinomas and in their precursors, known as dysplasia, intraepithelial neoplasia (cin) or squamous intraepithelial lesions (sil). the natural history of these cancer precursors is characterised by regr ... | 1998 | 10387956 |
| imiqimod in clinical practice. | imiquimod 5% cream is a new compound which modifies the immune response by stimulating the production of interferon alpha and other cytokines. it has shown remarkable promise in the treatment of external genital and perianal warts when applied overnight, three times a week. it is also associated with a lower recurrence than those found with other current treatments. this paper reviews two of the pivotal multi-centre studies which confirm its efficacy in human papilloma virus (hpv) infections. th ... | 1998 | 10387959 |
| the human papillomavirus e7 oncoprotein abrogates signaling mediated by interferon-alpha. | greater than 95% of all cervical carcinomas have been found to be associated with "high-risk" human papillomavirus (mainly types 16 and 18) infections, with the viral e6 and e7 oncoproteins essential for neoplastic development and maintenance. interferon-alpha (ifnalpha) is used in the treatment of hpv infections yet both in vivo and in vitro data suggest that the virus has developed mechanisms to avoid the effects of interferon. here we show that the hpv16 e7 oncoprotein is able to inhibit the ... | 1999 | 10388655 |
| uniform distribution of hpv 16 e6 and e7 variants in patients with normal histology, cervical intra-epithelial neoplasia and cervical cancer. | high-risk human papillomaviruses (hpv), particularly hpv 16, are associated with invasive cervical cancer (icc), and persistent high-risk hpv infection is considered to be a marker for progressive cervical intra-epithelial neoplasia (cin). however, most high-risk, hpv-infected, pre-cancerous lesions will not progress to invasion. several reports suggest that specific hpv 16 e6 and/or e7 sequence variations may be associated with a high risk for progression. no data from german patients have so f ... | 1999 | 10389753 |
| cloning of e6 and e7 genes of human papilloma virus type 18 and transformation potential of e7 gene and its mutants. | e6 and e7 genes of human papilloma virus type 18 have been subcloned from plasmid pc7, carrying an insert of dna from squamous cell carcinoma of cervix. both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. in the case of e6 gene this mutation is mapped in codon 129, in the case of e7 the same change aac to aaa mapped in codon 92. in addition both genes contain few point mutations that do not change the aminoacid sequences of the protein. two muta ... | 1999 | 10403700 |
| sialadenoma papilliferum of the palate: case report and literature review. | sialadenoma papilliferum (sp) is a rare tumor of salivary gland ducts which bears a strong histologic resemblance to the more common syringocystadenoma papilliferum (scap). we report a case occurring on the palate of a 50-year-old man, and review the clinical and histologic features of this tumor. because of the histologic similarities between these two tumors and squamous papillomas, polymerase chain reaction (pcr) for human papilloma virus (hpv) dna was performed on this tumor and on two cases ... | 1999 | 10408352 |
| screening for genital human papillomavirus: results from an international validation study on human papillomavirus sampling techniques. | the objective of this study was to determine the validity of human papillomavirus (hpv) detection using exfoliated cervical cells compared with cervical biopsy specimens in women with normal cervix and to assess whether hpv detection rates using exfoliated cells is dependent on the number and order in which cervical scrapes are taken. women undergoing hysterectomy for reasons other than cervical cancer were recruited in three hospitals in countries with varying risks of cervical cancer. after in ... | 1999 | 10408790 |
| human papillomavirus infection and other risk factors for cervical intraepithelial neoplasia in japan. | various risk factors were investigated in 167 cervical intra-epithelial neoplasia (cin) case and control pairs in japan. cin cases showed evidence of nine known risk factors including smoking and sexual behaviour. however, after adjustment for papillomavirus infection, the highest determinant, the only remaining risk factors were: being married, early age at first pregnancy and multiparity. | 1999 | 10408876 |
| establishment of an immortalized fetal intrapulmonary artery endothelial cell line. | the investigation of fetal pulmonary endothelial cell gene expression and function has been limited by the requirement for primary cells. in an effort to establish an immortalized cell line, ovine fetal pulmonary artery endothelial cells (paecs; passage 5) were permanently transfected with the e6 and e7 open reading frames of human papillomavirus type 16, and phenotypes related to nitric oxide (no) production were evaluated up to passage 28. acetylated low-density lipoprotein uptake, endothelial ... | 1999 | 10409236 |
| clinical, histopathologic, and molecular aspects of cutaneous human papillomavirus infections. | human papillomaviruses comprise a large family of double stranded dna viruses that are the etiologic agents of benign warts and anogenital cancers. at least 82 different human papillomavirus types have been identified and many remain yet uncharacterized. the development of new molecular techniques in recent years has led to an increased understanding of human papillomaviruses and their roles in carcinogenesis. several clinicopathologic entities arising from human papillomavirus infection encount ... | 1999 | 10410856 |
| human papillomavirus 18 oncoproteins e6 and e7 enhance irradiation- and chemotherapeutic agent-induced apoptosis in p53 and rb mutated cervical cancer cell lines. | tumor suppressor genes p53 and rb are important in cell cycle control. necessity of wild type p53 is implicated in irradiation-induced apoptosis. numerous tumor cells carry p53 mutations and yet are sensitive to irradiation or chemotherapeutic agents. therefore p53-and rb-independent pathways must exist to account for irradiation-induced apoptosis. we evaluated the apoptotic response of a p53- and rb-mutated, human papilloma virus (hpv) negative cervical cancer cell line (c33a), and c33a cell li ... | 1999 | 10410876 |
| genital bowen disease associated with an unusual human papillomavirus type 57b. | 1999 | 10411173 | |
| perianal bowen's disease and anal intraepithelial neoplasia: review of the literature. | the aim of this study was to review the literature with regard to perianal bowen's disease and anal intraepithelial neoplasia. | 1999 | 10411443 |
| human papillomavirus type 31 oncoproteins e6 and e7 are required for the maintenance of episomes during the viral life cycle in normal human keratinocytes. | the e6 and e7 oncoproteins of the high-risk human papillomavirus (hpv) types are able to immortalize human keratinocytes in vitro and likely contribute to the development of anogenital malignancies in vivo. the role of these oncoproteins in the productive viral life cycle, however, is not known. to begin to examine these possible roles, mutations in e6 were introduced in the context of the complete hpv 31 genome. although transfected wild-type hpv 31 genomes, as well as genomes containing an e6 ... | 1999 | 10411895 |
| short-term follow up of cervical squamous intraepithelial lesions associated with hiv and human papillomavirus infections in africa. | a prospective study in gynaecology clinics was conducted in abidjan, côte d'ivoire, to assess the short-term evolution of squamous intraepithelial lesions (sils). of 94 women with a cytological diagnosis of sil, 38 were infected with hiv. the average follow-up period after the initial smear was 5 months. detection of human papillomavirus (hpv) by polymerase chain reaction (pcr) was performed at both the time of enrolment and final follow-up smear. there were 39 cases of persistent sils. hiv-posi ... | 1999 | 10414878 |
| does plantar epidermoid cyst with human papillomavirus infection originate from the eccrine dermal duct? | 1999 | 10417539 | |
| mucosal oncogenic human papillomaviruses and extragenital bowen disease. | genital bowen disease is known to have a strong association with human papillomavirus (hpv) type 16. on the other hand, previous studies of extragenital bowen disease (ebd) that have used different hybridization techniques have produced discordant results in the detection of mucosal oncogenic hpv. | 1999 | 10421264 |
| pleiotropic effects of human papillomavirus type 16 e6 oncogene expression in human epithelial cell lines. | two human papillomavirus (hpv)-negative epithelial cell lines, hacat and c33a, were transfected with hpv-16 e6 and analysed for functional consequences which are relevant to invasive tumour progression. after transfection with e6, both cell lines invaded collagen matrices, in contrast to vector-transfected control cells. the e6-expressing cells showed a marked increase in expression of the beta1 integrin subunit, with no or relatively minor alterations in the levels of a range of integrin subuni ... | 1999 | 10423139 |
| collection devices for human papillomavirus. | human papillomavirus (hpv) testing has relied to date on samples collected by experienced health professionals. self-administered testing devices could allow hpv testing to occur in large-scale epidemiologic studies of primary care screening populations. the purpose of this study is to determine whether a self-collection device for cervicovaginal hpv infection could be developed. | 1999 | 10428251 |
| colposcopy in pregnancy: directed brush cytology compared with cervical biopsy. | to evaluate colposcopically directed brush cytology as a substitute for directed biopsy of acetowhite lesions identified during pregnancy. | 1999 | 10432127 |
| oncological complications of human immunodeficiency virus disease and hematologic consequences of their treatment. | despite advances in antiretroviral therapy and in the treatment and prevention of opportunistic infections, oncological and consequent hematologic complications of human immunodeficiency virus (hiv) infection continue to occur and are of significant clinical importance. virus-related tumors (e.g., kaposi's sarcoma, induced by human herpesvirus 8; non-hodgkin's lymphoma, linked to epstein-barr virus; and anogenital tumors, linked to human papillomavirus) are frequent in patients with hiv-induced ... | 1999 | 10433562 |
| mdm2 binds p73 alpha without targeting degradation. | the function of the p53 tumor suppressor protein is regulated by interaction with mdm2, which targets p53 for ubiquitin dependent degradation. we show here that like p53, p73 alpha forms an interaction with mdm2, both in vitro and in cells, but this does not result in the degradation of the p73 alpha protein. the human papillomavirus e6 protein also fails to degrade p73 alpha, suggesting that the mechanisms governing p73 alpha stability are distinct from those known to regulate p53 stability. ho ... | 1999 | 10435614 |
| expression of human papillomavirus 16 e2 protein in schizosaccharomyces pombe delays the initiation of mitosis. | infection by some types of human papillomavirus (hpv) is associated with the development of cervical cancer. analysis of viral dna from cervical tumours shows that the e2 gene is frequently disrupted during integration into the host cell's dna. it has therefore been suggested that loss of e2p is an important step in malignant transformation. expression of e2p in the fission yeast schizosaccharomyces pombe retards the g2-m transition, by delaying activation of cdc2p kinase. in contrast, s phase p ... | 1999 | 10435625 |
| regulation of human papillomavirus type 31 polyadenylation during the differentiation-dependent life cycle. | the l1 and l2 capsid genes of human papillomavirus type 31 (hpv-31) are expressed late in the differentiation-dependent life cycle from a promoter located in the e7 open reading frame (orf) of the early region. these late hpv genes are transcribed by rna polymerase ii which reads through the region containing early polyadenylation signals and proceeds to a poly(a) site downstream of l1. in this study, we have investigated the mechanisms regulating differentiation-dependent polyadenylation and re ... | 1999 | 10438805 |
| human papillomavirus type 16 e7 dna vaccine: mutation in the open reading frame of e7 enhances specific cytotoxic t-lymphocyte induction and antitumor activity. | a human papillomavirus type 16 e7 dna vaccine with the open reading frame encoding mutations in two zinc-binding motifs expressed a rapidly degraded e7 protein. this vaccine induced a significantly stronger e7-specific cytotoxic t-lymphocyte response and better tumor protection in mice than did a wild-type e7 dna vaccine expressing a stable e7 protein. | 1999 | 10438884 |
| nuclear import of hpv11 l1 capsid protein is mediated by karyopherin alpha2beta1 heterodimers. | l1 major capsid proteins of human papillomaviruses (hpvs) enter the nuclei of host cells at two times during the viral life cycle: 1) after infection and 2) later during the productive phase, when they assemble the replicated hpv genomic dna into infectious virions. l1 proteins are stable in two oligomeric configurations: as homopentameric capsomers, and as capsids composed of 72 capsomers. we found that intact l1 capsids of hpv type 11 cannot enter the nucleus, suggesting that capsid disassembl ... | 1999 | 10440932 |
| new concepts on the role of human papillomavirus in cell cycle regulation. | human papillomaviruses (hpvs) are strictly host-specific and also show a distinct tropism to squamous epithelial cells. upon hpv infection, only a portion of the virus reaching the nucleus seems to undergo replication, suggesting that hpv replication remains confined to a small number of cells. hpvs critically depend on the cellular machinery for the replication of their genome. viral replication is restricted to differentiated keratinocytes that are normally growth arrested. hence, hpvs have de ... | 1999 | 10442672 |
| [detection of human papillomavirus types in tumors and inflammations of the eye]. | infections by the human papillomavirus occur in the skin and in the mucosa. they may play a significant role in the neoplastic transformation of cells. at least half of all the epithelial tumours of the ocular surface and the lacrimal drainage system have been shown to be hpv associated. however, in ca. 50% of these tumors only a limited number of mucosal hpv types could be detected by commercially available test systems. this study reports of hpv specific sequences in putatively hpv associated ... | 1999 | 10448632 |