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influence of breeding site availability on the oviposition behaviour of aedes aegypti.despite the importance of the mosquito aedes aegypti in the transmission of arboviruses, such as yellow fever, chikungunya fever and dengue fever, some aspects of their behaviour remain unknown. in the present study, the oviposition behaviour of ae. aegypti females that were exposed to different densities of breeding sites (2, 4, 8 and 16) was evaluated in laboratory and semi-field conditions. the number of breeding sites that were used was proportional to the number available, but tended toward ...201526154742
a trypsin inhibitor from clitoria fairchildiana cotyledons is active against digestive enzymes of aedes aegypti larvae.aedes aegypti, the principal mosquito vector of yellow fever, dengue fever and chikungunya fever virus-transmitted diseases, is an insect closely associated with humans and their housing habitats. as there is no commercially available vaccine, prevention is the most suggested form of avoiding disease spreading and a number of studies are being developed in order to give support to vector control operations. the present study reports on the identification of a trypsin inhibitor isolated from coty ...201526156641
the global compendium of aedes aegypti and ae. albopictus occurrence.aedes aegypti and ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. despite being pathogens of global public health importance, knowledge of their vectors' global distribution remains patchy and sparse. a global geographic database of known occurrences of ae. aegypti and ae. albopictus between 1960 and 2014 was compiled. herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and un ...201526175912
web platform using digital image processing and geographic information system tools: a brazilian case study on dengue.dengue fever is endemic in asia, the americas, the east of the mediterranean and the western pacific. according to the world health organization, it is one of the diseases of greatest impact on health, affecting millions of people each year worldwide. a fast detection of increases in populations of the transmitting vector, the aedes aegypti mosquito, is essential to avoid dengue outbreaks. unfortunately, in several countries, such as brazil, the current methods for detecting populations changes ...201526178732
characterization and biotoxicity of hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against aedes aegypti and plutella xylostella.two of the most important challenges facing humanity in the 21st century comprise food production and disease control. eco-friendly control tools against mosquito vectors and agricultural pests are urgently needed. insecticidal products of marine origin have a huge potential to control these pests. in this research, we reported a single-step method to synthesize silver nanoparticles (agnp) using the aqueous leaf extract of the seaweed hypnea musciformis, a cheap, nontoxic and eco-friendly materi ...201526184431
tree holes as larval habitats for aedes aegypti in urban, suburban and forest habitats in a dengue affected area.aedes aegypti (l.) (diptera: culicidae), the main vector of dengue and urban yellow fever in the world, is highly adapted to the human environment. artificial containers are the most common larval habitat for the species, but it may develop in tree holes and other phytotelmata. this study assessed whether tree holes in san ramón de la nueva orán, a city located in subtropical montane moist forest where dengue outbreaks occur, are relevant as larval habitat for ae. aegypti and if the species may ...201526193903
application of wmelpop wolbachia strain to crash local populations of aedes aegypti.the endosymbiotic bacteria wolbachia pipientis (wmel strain) has been successfully established in several populations of aedes aegypti, the primary dengue vector. the virulent wolbachia strain wmelpop is known to cause several pathological impacts (increased egg mortality, life shortening, etc.) reducing overall fitness in the mosquito ae. aegypti. increased egg mortality could substantially reduce egg banks in areas with a lengthy monsoonal dry season, and be employed to eliminate local populat ...201526204449
identifying genomic changes associated with insecticide resistance in the dengue mosquito aedes aegypti by deep targeted sequencing.the capacity of mosquitoes to resist insecticides threatens the control of diseases such as dengue and malaria. until alternative control tools are implemented, characterizing resistance mechanisms is crucial for managing resistance in natural populations. insecticide biodegradation by detoxification enzymes is a common resistance mechanism; however, the genomic changes underlying this mechanism have rarely been identified, precluding individual resistance genotyping. in particular, the role of ...201526206155
host-feeding pattern of aedes aegypti and aedes albopictus (diptera: culicidae) in heterogeneous landscapes of south andaman, andaman and nicobar islands, india.mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. competent vectors of dengue and chikungunya viz. aedes aegypti and aedes albopictus are widely prevalent in the andaman and nicobar archipelago. considering the vector potential, medical importance of both these mosquito species and lack of information on ho ...201526220560
extracellular synthesis of silver nanoparticles using bacillus megaterium against malarial and dengue vector (diptera: culicidae).biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. in the present study, silver nanoparticles (agnps) were synthesized extracellular method using bacillus megaterium. the agnps formations were confirmed initially through color change, and the aliquots were characterized through uv-visible spectrophotometer, followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy, and fourier transform infrared (ftir) ...201526231835
[neonatal dengue. presentation of clinical cases].dengue is an infectious disease caused by a flavivirus, with four serotypes, transmitted by the mosquito aedes aegypti. in mexico it is a public health problem, especially in the region of central and southeast of the country. the disease can be asymptomatic or present serious forms and even death. it is confirmed by detection of the ns1 antigen; igm antibodies, polymerase chain reaction and virus isolation. the vertical transmission to de newborn has been little studied. 7 cases in neonates fro ...201526233977
cry4ba and cyt1aa proteins from bacillus thuringiensis israelensis: interactions and toxicity mechanism against aedes aegypti.individual crystal proteins from bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. in all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. in the present study we investigated the effects of cyt1a98 (a cyt1aa type protein) on cry4blb (a cry4ba type toxin) insecticidal activity toward the dengue vector aedes aegypti. the bioassay analyses demonstrated t ...201526238170
the genetic architecture of a complex trait: resistance to multiple toxins produced by bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito aedes aegypti.the bacterial insecticide bacillus thuringiensis subsp. israelensis (bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. because bti toxicity relies on the action of four main toxins, resistance to bti is very likely a complex phenotype involving several genes simultaneously. dissecting the underlying genetic basis thus requires associating a quantitative measure of resistance to genetic variation at many loci in a segregating population. he ...201526238211
forced egg retention and oviposition behavior of malaria, dengue and filariasis vectors to a topical repellent diethyl-phenylacetamide.egg retention and oviposition behavior of four species of mosquito vectors viz., anopheles stephensi, aedes aegypti, aedes albopictus, and culex quinquefasciatus to a topical insect repellent diethyl-phenylacetamide (depa) at 0.1-1000 mg/l was investigated under laboratory conditions. based on oviposition activity indices, depa demonstrated concentration dependent oviposition deterrent effect to a. stephensi (-0.18 to -0.97), a. aegypti (-0.18 to -0.91) and a. albopictus (-0.50 to -0.98) females ...201526245028
effect of chloroxylon swietenia dc bark extracts against culex quinquefasciatus, aedes aegypti, and anopheles stephensi larvae.mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. this article deals with the mosquito larvicidal activity of chloroxylon swietenia dc bark extracts against late third instar larvae of culex quinquefasciatus, aedes aegypti, and anopheles stephensi. methanolic crude extract of ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. the range of concentrations of ...201526246308
mosquitocidal and oviposition repellent activities of the extracts of seaweed bryopsis pennata on aedes aegypti and aedes albopictus.the ever-increasing threat from infectious diseases and the development of insecticide resistance in mosquito populations drive the global search for new natural insecticides. the aims of this study were to evaluate the mosquitocidal activity of the extracts of seaweed bryopsis pennata against dengue vectors aedes aegypti and aedes albopictus, and determine the seaweed's toxic effect on brine shrimp nauplii (as a non-target organism). in addition, the chemical compositions of the active larvicid ...201526247928
remarkable repellency of ligusticum sinense (umbelliferae), a herbal alternative against laboratory populations of anopheles minimus and aedes aegypti (diptera: culicidae).for personal protection against mosquito bites, user-friendly natural repellents, particularly from plant origin, are considered as a potential alternative to applications currently based on synthetics such as deet, the standard chemical repellent. this study was carried out in thailand to evaluate the repellency of ligusticum sinense hexane extract (lhe) against laboratory anopheles minimus and aedes aegypti, the primary vectors of malaria and dengue fever, respectively.201526249666
parity and longevity of aedes aegypti according to temperatures in controlled conditions and consequences on dengue transmission risks.in guadeloupe, aedes aegypti mosquitoes are the only vectors of dengue and chikungunya viruses. for both diseases, vector control is the only tool for preventing epidemics since no vaccine or specific treatment is available. however, to efficiently implement control of mosquitoes vectors, a reliable estimation of the transmission risks is necessary. to become infective an ae. aegypti female must ingest the virus during a blood meal and will not be able to transmit the virus during another blood- ...201526258684
diversity and abundance of mosquitoes (diptera:culicidae) in an urban park: larval habitats and temporal variation.urban parks are areas designated for human recreation but also serve as shelter and refuge for populations of several species of native fauna, both migratory and introduced. in brazil, the effect of annual climate variations on aedes aegypti and dengue epidemics in large cities like são paulo is well known, but little is known about how such variations can affect the diversity of mosquito vectors in urban parks and the risk of disease transmission by these vectors. this study investigates the in ...201526259817
mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action.mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ...201526267052
assessment of the impact of potential tetracycline exposure on the phenotype of aedes aegypti ox513a: implications for field use.aedes aegypti is the primary vector of dengue fever, a viral disease which has an estimated incidence of 390 million infections annually. conventional vector control methods have been unable to curb the transmission of the disease. we have previously reported a novel method of vector control using a tetracycline repressible self-limiting strain of ae. aegypti ox513a which has achieved >90% suppression of wild populations.201526270533
candidate biomarkers for mosquito age-grading identified by label-free quantitative analysis of protein expression in aedes albopictus females.we applied a "shotgun" approach based on nanoliquid chromatography-high resolution mass spectrometry associated to label free quantification (lfq) to identify proteins varying with age, independently from the physiological state, in aedes albopictus, a mosquito species which in the last decades invaded temperate regions in north america and europe, creating concerns for associated high nuisance and risk of arbovirus transmission. the combined "shotgun" and lfq approach was shown to be highly sui ...201526271156
meteorologically driven simulations of dengue epidemics in san juan, pr.meteorological factors influence dengue virus ecology by modulating vector mosquito population dynamics, viral replication, and transmission. dynamic modeling techniques can be used to examine how interactions among meteorological variables, vectors and the dengue virus influence transmission. we developed a dengue fever simulation model by coupling a dynamic simulation model for aedes aegypti, the primary mosquito vector for dengue, with a basic epidemiological susceptible-exposed-infectious-re ...201526275146
heme signaling impacts global gene expression, immunity and dengue virus infectivity in aedes aegypti.blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. heme is a pro-oxidant that influences a variety of cellular processes. we performed a global analysis of heme-regulated aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. we observed an iron- and reactive oxygen species (ros)-independent signaling induced by heme that comprised genes related to redox metabolis ...201526275150
role of aedes aegypti and aedes albopictus during the 2011 dengue fever epidemics in hanoi, vietnam.to record the human cases of dengue fever (df) and investigate the aedes mosquito species circulating during the hanoi 2011 df epidemics.201526276285
physiological and biochemical effects of botanical extract from piper nigrum linn (piperaceae) against the dengue vector aedes aegypti liston (diptera: culicidae).the leaves of piper nigrum l. (piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of aedes aegypti. gc and gc-ms analyses revealed that the crude extracts contain 16 compounds. thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3r-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3h)-furanone, 3,4-bi ...201526277727
rapid biosynthesis of silver nanoparticles using crotalaria verrucosa leaves against the dengue vector aedes aegypti: what happens around? an analysis of dragonfly predatory behaviour after exposure at ultra-low doses.aedes aegypti is a primary vector of dengue, a mosquito-borne viral disease infecting 50-100 million people every year. here, we biosynthesised mosquitocidal silver nanoparticles (agnp) using the aqueous leaf extract of crotalaria verrucosa. the green synthesis of agnp was studied by uv-vis spectroscopy, sem, edx and ftir. c. verrucosa-synthesised agnps were toxic against a. aegypti larvae and pupae. lc50 of agnp ranged from 3.496 ppm (i instar larvae) to 17.700 ppm (pupae). furthermore, we eval ...201626284510
nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae).mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. dengue is a viral disease mainly vectored through the bite of aedes mosquitoes. its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depend on effective vector control measures. mangrov ...201526290219
reemergence and autochthonous transmission of dengue virus, eastern china, 2014.in 2014, 20 dengue cases were reported in the cities of wenzhou (5 cases) and wuhan (15 cases), china, where dengue has rarely been reported. dengue virus 1 was detected in 4 patients. although most of these cases were likely imported, epidemiologic analysis provided evidence for autochthonous transmission.026292098
widespread evidence for interspecific mating between aedes aegypti and aedes albopictus (diptera: culicidae) in nature.aedes aegypti and aedes albopictus, two important vectors of the dengue and chikungunya viruses to humans, often come in contact in their invasive ranges. in these circumstances, a number of factors are thought to influence their population dynamics, including resource competition among the larval stages, prevailing environmental conditions and reproductive interference in the form of satyrization. as the distribution and abundance of ae. aegypti and ae. albopictus have profound epidemiological ...201526296606
further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from myracrodruon urundeuva seeds against aedes aegypti.nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector aedes aegypti. besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. thus, the present wor ...201526299195
eco-friendly control of malaria and arbovirus vectors using the mosquitofish gambusia affinis and ultra-low dosages of mimusops elengi-synthesized silver nanoparticles: towards an integrative approach?mosquito-borne diseases represent a deadly threat for millions of people worldwide. however, the use of synthetic insecticides to control culicidae may lead to high operational costs and adverse non-target effects. plant-borne compounds have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles. their impact against biological control agents of mosquito larval populations has been poorly studied. we synthesized silver nanoparticles (agnp) using the aqueous leaf extract o ...201526300364
[control of aedes aegypti breeding sites with the program recicla por tu bienestar in merida, mexico].to determine the importance of ae. aegypti breeding-sites in merida;to evaluate the impact of recicla por tu bienestar (rxb, a recycling program) on the reduction of breeding sites and the perception of participants.201726302122
susceptibility profile of aedes aegypti from santiago island, cabo verde, to insecticides.in 2009, cabo verde diagnosed the first dengue cases, with 21,137 cases reported and aedes aegypti was identified as the vector. since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. this study aimed to assess the susceptibility of a. aegypti populations from santiago, cabo verde to insecticides and identify the mechanisms of resistance. samples of a. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in ...201526307496
tracking dengue virus intra-host genetic diversity during human-to-mosquito transmission.dengue virus (denv) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. this intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. to track changes in viral intra-host genetic diversity during horizontal transmission, we infected aedes aegypti mosquitoes by allowing them to feed on denv2-infected patients. we then p ...201526325059
detection of dengue virus in mosquito extracts and human clinical samples using a field expedient molecular platform.dengue fever occurs in localized outbreaks and can significantly erode troop strength and mission readiness. timely identification of dengue virus (denv) provides for rapid and appropriate patient management decisions, such as medical evacuation and supportive therapies, as well as help to promote force health protection through vector control and personal protective measures. the "ruggedized" advanced pathogen identification device is a field-friendly pcr (polymerase chain reaction) platform th ...201526327544
evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected aedes aegypti and aedes albopictus (diptera: culicidae).the simultaneous transmission of chikungunya virus (chikv) and dengue viruses (denv) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. groups of aedes aegypti (l.) and aedes albopictus (skuse) were orally infected with 1.5 × 10(5) pfu/ml of chikv and 3.2 × 10(6) ffu/ml of denv-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qrt-pcr. simultaneous dissemination of both viruses was detecte ...201526334820
seasonal genetic changes of aedes aegypti (diptera: culicidae) populations in selected sites of cebu city, philippines.aedes aegypti (l.) is the primary vector of dengue virus in the philippines, where dengue is endemic. we examined the genetic changes of ae. aegypti collected from three selected sites in cebu city, philippines, during the relatively wet (2011-2012) and dry seasons (2012 and 2013). a total of 493 ae. aegypti adults, reared in the laboratory from field-collected larvae, were analyzed using 11 microsatellite loci. seasonal variation was observed in allele frequencies and allelic richness. average ...201526335470
evaluating liquid and granular bacillus thuringiensis var. israelensis broadcast applications for controlling vectors of dengue and chikungunya viruses in artificial containers and tree holes.the principal vectors of chikungunya and dengue viruses typically oviposit in water-filled artificial and natural containers, including tree holes. despite the risk these and similar tree hole-inhabiting mosquitoes present to global public health, surprisingly few studies have been conducted to determine an efficient method of applying larvicides specifically to tree holes. the stihl sr 450, a backpack sprayer commonly utilized during military and civilian vector control operations, may be suita ...201526335473
introduction and establishment of aedes albopictus (diptera: culicidae) in managua, nicaragua.aedes aegypti (l.) is the main vector of dengue virus and more recently chikungunya virus in latin america. however, the asian tiger mosquito aedes albopictus (skuse, 1894) is expanding its global range and increasing its role in transmission of these diseases. in this report, we suggest that ae. albopictus was introduced to the department of managua, nicaragua, in 2010 via two independent routes and demonstrate its dissemination and establishment in urban neighborhoods by 2012. the coexistence ...201526335479
evidence for aedes aegypti (diptera: culicidae) oviposition on boats in the peruvian amazon.dengue vector aedes aegypti l. is invading peri-urban and rural areas throughout latin america. our previous research in the peruvian amazon has shown that river boats are heavily infested with immature and adult ae. aegypti mosquitoes, likely playing a major role in their long-distance dispersal and successful invasion. however, the presence of immature mosquitoes provides no information about the timing of oviposition, and whether it took place in the boats. here, we used baited ovitraps deplo ...201526335482
aedes aegypti (diptera: culicidae) in mauritania: first report on the presence of the arbovirus mosquito vector in nouakchott.aedes aegypti l. (diptera: culicidae) is a major vector of yellow fever, dengue, and chikungunya viruses throughout tropical and subtropical areas of the world. although the southernmost part of mauritania along the senegal river has long been recognized at risk of yellow fever transmission, aedes spp. mosquitoes had never been reported northwards in mauritania. here, we report the first observation of aedes aegypti aegypti (l.) and aedes (ochlerotatus) caspius (pallas, 1771) in the capital city ...201526335483
comparison of the insecticidal characteristics of commercially available plant essential oils against aedes aegypti and anopheles gambiae (diptera: culicidae).aedes aegypti and anopheles gambiae are two mosquito species that represent significant threats to global public health as vectors of dengue virus and malaria parasites, respectively. although mosquito populations have been effectively controlled through the use of synthetic insecticides, the emergence of widespread insecticide-resistance in wild mosquito populations is a strong motivation to explore new insecticidal chemistries. for these studies, ae. aegypti and an. gambiae were treated with c ...201526336230
sterol carrier protein 2, a critical host factor for dengue virus infection, alters the cholesterol distribution in mosquito aag2 cells.host factors that enable dengue virus (denv) to propagate in the mosquito host cells are unclear. it is known that cellular cholesterol plays an important role in the life cycle of denv in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. in mosquito aedes aegypti, sterol carrier protein 2 (scp-2) is critical for cellular cholesterol homeostasis. in this study, we identified scp-2 as a critical host factor for denv production in mosquito aag2 cells. tre ...201526336241
cold season mortality under natural conditions and subsequent hatching response of aedes (stegomyia) aegypti (diptera: culicidae) eggs in a subtropical city of argentina.in temperate and subtropical regions, populations of aedes (stegomyia) aegypti (l.) survive unfavorable winter conditions in the egg stage. knowing their survival rates can be of great interest for the health authorities in charge of control activities. in this study, we analyzed the mortality of ae. aegypti eggs exposed to the cold season as well as their hatching patterns under laboratory conditions in the city of resistencia, chaco, argentina. the mortality rate was 48.6%. no statistically si ...201526336247
interspecific larval competition differentially impacts adult survival in dengue vectors.mosquitoes often experience intraspecific and interspecific competition among larvae attributable to high densities and nutrient limitation, especially container mosquitoes including aedes aegypti (l.) and aedes albopictus (skuse). density-dependent effects on larvae impact adult production and adult traits that influence transmission of arboviruses. to improve our understanding of the mechanisms by which density-dependence influences transmission and identify species-specific traits, we tested ...201526336301
meta-analysis of studies on chemical, physical and biological agents in the control of aedes aegypti.aedes aegypti is a vector of international concern because it can transmit to humans three important arboviral diseases: yellow fever, dengue and chikungunya. epidemics that are repeated year after year in a variety of urban centers indicate that there are control failures, allowing the vector to continue expanding.201526341708
studies on insecticide susceptibility of aedes aegypti (linn) and aedes albopictus (skuse) vectors of dengue and chikungunya in andaman and nicobar islands, india.dengue and chikungunya are important arboviral infections in the andaman islands. competent vectors viz. aedes aegypti and aedes albopictus are widely prevalent. the most effective proven method for interrupting the transmission of these arboviruses is vector control, mediated through insecticides. currently, ddt and temephos are the insecticides used for vector control in these islands. lack of information on susceptibility necessitated assessing the susceptibility profile of a. aegypti and a. ...201526344869
behavioral response of aedes aegypti (diptera: culicidae) larvae to synthetic and natural attractants and repellents.aedes aegypti (l.) (diptera: culicidae) is the key vector of three important arboviral diseases: dengue, yellow fever, and chikungunya. immature stages of this species inhabit human-made containers placed in residential landscapes. in this study, we evaluated a few compounds in a sensitive behavioral assay with ae. aegypti larvae. the orientation of larvae to different compounds was surveyed using a performance index (pi). the pi represents the response to each odorant, where a value of +1 is in ...201526352935
microevolution of aedes aegypti.scientific research into the epidemiology of dengue frequently focuses on the microevolution and dispersion of the mosquito aedes aegypti. one of the world's largest urban agglomerations infested by ae. aegypti is the brazilian megalopolis of sao paulo, where >26,900 cases of dengue were reported until june 2015. unfortunately, the dynamics of the genetic variability of ae. aegypti in the sao paulo area have not been well studied. to reduce this knowledge gap, we assessed the morphogenetic varia ...201526360876
a multiobjective optimization approach for combating aedes aegypti using chemical and biological alternated step-size control.dengue epidemics, one of the most important viral disease worldwide, can be prevented by combating the transmission vector aedes aegypti. in support of this aim, this article proposes to analyze the dengue vector control problem in a multiobjective optimization approach, in which the intention is to minimize both social and economic costs, using a dynamic mathematical model representing the mosquitoes' population. it consists in finding optimal alternated step-size control policies combining che ...201526362231
a draft genome sequence of an invasive mosquito: an italian aedes albopictus.the draft genome sequence of italian specimens of the asian tiger mosquito aedes (stegomyia) albopictus (diptera: culicidae) was determined using a standard ngs (next generation sequencing) approach. the size of the assembled genome is comparable to that of aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive dna is concerned, most of which is made up of transposable elements. although, based on busco (benchmarking universal single-copy orthologues) analysi ...201526369436
use of centrifugal filter devices to concentrate dengue virus in mosquito per os infection experiments.dengue virus (denv) is an arbovirus transmitted to humans by the bite of infected aedes mosquitoes. experimental per os infection of mosquitoes with denv is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. we report here the convenient use of centrifugal filter devices to quickly concentrate denv particles in cell-culture supernatants. the median viral titer in concentrated-supernatants was 8.50 log10 tcid50/m ...201526372347
short-term suppression of aedes aegypti using genetic control does not facilitate aedes albopictus.under permit from the national biosafety commission for the use of genetically modified organisms, releases of a genetically engineered self-limiting strain of aedes aegypti (ox513a) were used to suppress urban pest ae. aegypti in west panama. experimental goals were to assess the effects on a coexisting population of ae. albopictus and examine operational parameters with relevance to environmental impact.201526374668
schools as potential risk sites for vector-borne disease transmission: mosquito vectors in rural schools in two municipalities in colombia.dengue and other vector-borne diseases are of great public health importance in colombia. vector surveillance and control activities are often focused at the household level. little is known about the importance of nonhousehold sites, including schools, in maintaining vector-borne disease transmission. the objectives of this paper were to determine the mosquito species composition in rural schools in 2 municipalities in colombia and to assess the potential risk of vector-borne disease transmissi ...201526375902
assessing carbon dioxide and synthetic lure-baited traps for dengue and chikungunya vector surveillance (3).the aedes mosquito vectors of dengue virus (denv) and chikungunya virus (chikv) are attracted to specific host cues that are not generated by traditional light traps. for this reason multiple companies have designed traps to specifically target those species. recently the standard trap for denv and chikv vectors, the bg-sentinel (bgs) trap, has been remodeled to be more durable and better suited for use in harsh field conditions, common during military operations, and relabeled the bg-sentinel 2 ...201526375905
arbovirus surveillance and first report of chikungunya virus in wild populations of aedes aegypti from guerrero, mexico.we carried out dengue (denv) and chikungunya virus (chikv) surveillance in wild populations of aedes aegypti from guerrero, mexico, from 2012 to 2014 following a standard national protocol of the mexican dengue control program. a total of 284 pools (15-30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase-polymerase chain reaction to detect denv and chikv. we report for the 1st time the detection of chikv from field-collected mosquitoes at acapulco and juchitán ...201526375910
risky behaviors: effects of toxorhynchites splendens (diptera: culicidae) predator on the behavior of three mosquito species.viable biocontrol agents for mosquito control are quite rare, therefore improving the efficacy of existing biological agents is an important study. we need to have a better understanding of the predation-risk behavioral responses toward prey. this research examined prey choices by toxorhynchites splendens by monitoring the behavioral responses of aedes aegypti, aedes albopictus, and anopheles sinensis larvae when exposed to the predator. the results show that tx. splendens prefers to consume ae. ...201526386041
oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions.the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ...201526399712
public health response to aedes aegypti and ae. albopictus mosquitoes invading california, usa.aedes aegypti and ae. albopictus mosquitoes, primary vectors of dengue and chikungunya viruses, were recently detected in california, usa. the threat of potential local transmission of these viruses increases as more infected travelers arrive from affected areas. public health response has included enhanced human and mosquito surveillance, education, and intensive mosquito control.026401891
erratum to: nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae). 201526412056
control of aedes aegypti and ae. albopictus, the vectors of dengue and chikungunya, by using pheromone c21 with an insect growth regulator: results of multicentric trials from 2007-12 in india.aedes mosquito control has gained much importance nowadays in view of rise in number of reported cases of dengue and chikungunya in india and other countries. in the present study, c21 attracticide (containing a pheromone and an insect growth regulator—igr, developed by defence research and development establishment (drde), gwalior, india was tested for its feasibility for surveillance and control of aedes mosquito in a multicentric mode from october 2007 to june 2012 in urban (delhi, and bengal ...201526418653
breeding sites of aedes aegypti: potential dengue vectors in dire dawa, east ethiopia.background and objectives. entomological survey was carried out from may-june to september-october 2014 to investigate the presence of dengue vectors in discarded tires and artificial water containers in houses and peridomestic areas. methods. a cross-sectional immature stage survey was done indoors and outdoors in 301 houses. mosquito larval sampling was conducted using pipette or dipper depending on container types. larvae were identified morphologically and larval indices were also calculated ...201526435712
permethrin-treated clothing as protection against the dengue vector, aedes aegypti: extent and duration of protection.dengue transmission by the mosquito vector, aedes aegypti, occurs indoors and outdoors during the day. personal protection of individuals, particularly when outside, is challenging. here we assess the efficacy and durability of different types of insecticide-treated clothing on laboratory-reared ae. aegypti.201526440967
production of serine protease inhibitors by mutagenesis and their effects on the mortality of aedes aegypti l. larvae.dengue, transmitted primarily by the bites of infected aedes aegypti l., is transmitted to millions of individuals each year in tropical and subtropical areas. dengue control strategies are primarily based on controlling the vector, using insecticides, but the appearance of resistance poses new challenges. recently, highly selective protease inhibitors by phage display were obtained for digestive enzymes of the 4th instar larvae (l4) midgut. these mutants were not confirmed as a larvicide due to ...201526444542
acetylcholinesterases from the disease vectors aedes aegypti and anopheles gambiae: functional characterization and comparisons with vertebrate orthologues.mosquitoes of the anopheles (an.) and aedes (ae.) genus are principal vectors of human diseases including malaria, dengue and yellow fever. insecticide-based vector control is an established and important way of preventing transmission of such infections. currently used insecticides can efficiently control mosquito populations, but there are growing concerns about emerging resistance, off-target toxicity and their ability to alter ecosystems. a potential target for the development of insecticide ...201526447952
fighting arboviral diseases: low toxicity on mammalian cells, dengue growth inhibition (in vitro), and mosquitocidal activity of centroceras clavulatum-synthesized silver nanoparticles.dengue is a mosquito-borne viral disease that has rapidly spread in all regions of the world in recent years. female mosquitoes, mainly aedes aegypti, transmit dengue. approximately 3,900 million people, in 128 countries, are at risk of dengue infection. recently, a focus has been provided on the potential of green-synthesized nanoparticles as inhibitors of the production of dengue viral envelope (e) protein in vero cells and downregulators of the expression of dengue viral e gene. algae are an ...201626462804
dengue in florida (usa).florida (usa), particularly the southern portion of the state, is in a precarious situation concerning arboviral diseases. the geographic location, climate, lifestyle, and the volume of travel and commerce are all conducive to arbovirus transmission. during the last decades, imported dengue cases have been regularly recorded in florida, and the recent re-emergence of dengue as a major public health concern in the americas has been accompanied by a steady increase in the number of imported cases. ...201426462955
aedes aegypti control strategies in brazil: incorporation of new technologies to overcome the persistence of dengue epidemics.dengue is considered to be the most important mosquito-borne viral disease in the world. the aedes aegypti mosquito, its vector, is highly anthropophilic and is very well adapted to urban environments. although several vaccine candidates are in advanced stages of development no licensed dengue vaccine is yet available. as a result, controlling the spread of dengue still requires that mosquitoes be targeted directly. we review the current methods of dengue vector control focusing on recent techni ...201526463204
association between three mutations, f1565c, v1023g and s996p, in the voltage-sensitive sodium channel gene and knockdown resistance in aedes aegypti from yogyakarta, indonesia.mutations in the voltage-sensitive sodium channel gene (vssc) have been identified in aedes aegypti and some have been associated with pyrethroid insecticide resistance. whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if mutations are to become markers for resistance monitoring. we describe high resolution melt (hrm) genotyping assays for assessing mutations found in ae. aegypti in indonesia (f1565c, v1023g, s996p) and ...201526463408
copulation activity, sperm production and conidia transfer in aedes aegypti males contaminated by metarhizium anisopliae: a biological control prospect.dengue is the most prevalent arboviral disease transmitted by aedes aegypti worldwide, whose chemical control is difficult, expensive, and of inconsistent efficacy. releases of metarhizium anisopliae--exposed ae. aegypti males to disseminate conidia among female mosquitoes by mating represents a promising biological control approach against this important vector. a better understanding of fungus virulence and impact on reproductive parameters of ae. aegypti, is need before testing auto-dissemina ...201526473490
a role for vector control in dengue vaccine programs.development and deployment of a successful dengue virus (denv) vaccine has confounded research and pharmaceutical entities owing to the complex nature of denv immunity and concerns over exacerbating the risk of denv hemorrhagic fever (dhf) as a consequence of vaccination. thus, consensus is growing that a combination of mitigation strategies will be needed for denv to be successfully controlled, likely involving some form of vector control to enhance a vaccine program. we present here a determin ...201526478199
larval temperature-food effects on adult mosquito infection and vertical transmission of dengue-1 virus.temperature-food interactions in the larval environment can affect life history and population growth of container mosquitoes aedes aegypti (l.) and aedes albopictus skuse, the primary vectors of chikungunya and dengue viruses. we used ae. aegypti, ae. albopictus, and dengue-1 virus (denv-1) from florida to investigate whether larval rearing temperature can alter the effects of larval food levels on ae. aegypti and ae. albopictus life history and denv-1 infection and vertical transmission. altho ...201526489999
dengue virus infection of aedes aegypti requires a putative cysteine rich venom protein.dengue virus (denv) is a mosquito-borne flavivirus that causes serious human disease and mortality worldwide. there is no specific antiviral therapy or vaccine for denv infection. alterations in gene expression during denv infection of the mosquito and the impact of these changes on virus infection are important events to investigate in hopes of creating new treatments and vaccines. we previously identified 203 genes that were ≥5-fold differentially upregulated during flavivirus infection of the ...201526491875
contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from rio de janeiro: implications for vector control.dengue is the most prevalent global arboviral disease that affects over 300 million people every year. brazil has the highest number of dengue cases in the world, with the most severe epidemics in the city of rio de janeiro (rio). the effective control of dengue is critically dependent on the knowledge of population genetic structuring in the primary dengue vector, the mosquito aedes aegypti. we analyzed mitochondrial and nuclear genomewide single nucleotide polymorphism markers generated via re ...201526495042
dengue knowledge and preventive practices in iquitos, peru.as part of a cluster-randomized trial to evaluate insecticide-treated curtains for dengue prevention in iquitos, peru, we surveyed 1,333 study participants to examine knowledge and reported practices associated with dengue and its prevention. entomological data from 1,133 of these households were linked to the survey. most participants knew that dengue was transmitted by mosquito bite (85.6%), but only few (18.6%) knew that dengue vectors bite during daytime. most commonly recognized dengue symp ...201526503276
dengue fever and dengue hemorrhagic fever in adults.dengue fever and dengue hemorrhagic fever are re-emerging diseases that are endemic in the tropics. the global prevalence of dengue cases has increased in south-east asia, africa, the western pacific, and the americas. the increasingly widespread distribution and the rising incidence of dengue virus infections are related to increased distribution of aedes aegypti, an increasingly urban population, and increasing air travel. several southeast asian countries show that the age of the reported den ...201526506734
field evaluation of the establishment potential of wmelpop wolbachia in australia and vietnam for dengue control.introduced wolbachia bacteria can influence the susceptibility of aedes aegypti mosquitoes to arboviral infections as well as having detrimental effects on host fitness. previous field trials demonstrated that the wmel strain of wolbachia effectively and durably invades ae. aegypti populations. here we report on trials of a second strain, wmelpop-pgyp wolbachia, in field sites in northern australia (machans beach and babinda) and central vietnam (tri nguyen, hon mieu island), each with contrasti ...201526510523
dengue virus infection alters post-transcriptional modification of micrornas in the mosquito vector aedes aegypti.recent discoveries regarding the importance of isomirs have increased our understanding of the regulatory complexities of the mirnaome. observed changes in the mirna profiles in mosquitoes infected with flaviviruses have implicated small rnas in the interactions between viruses and their vectors. here we analysed the isomir profiles of both uninfected and infected aedes aegypti mosquitoes with the major human pathogen dengue virus (denv). we found that several specific isomirs were significantly ...201526514826
nepenthes ampullaria (nepenthaceae) pitchers are unattractive to gravid aedes aegypti and aedes albopictus (diptera: culicidae).nepenthes pitcher plants are colonized by a variety of specialized arthropods. as aedes mosquitoes are container breeders, nepenthes pitchers are a potential candidate oviposition site for vector species, such as aedes aegypti (l.) and aedes albopictus (skuse). however, aedes spp. are not commonly encountered in nepenthes pitchers, and the environment inside the pitchers of some species is lethal to them. one exception is nepenthes ampullaria jack, whose pitchers are known to be colonized by ae. ...201626518035
historical inability to control aedes aegypti as a main contributor of fast dispersal of chikungunya outbreaks in latin america.the arrival of chikungunya fever (chikf) in latin american countries has been expected to trigger epidemics and challenge health systems. historically considered as dengue-endemic countries, abundant aedes aegypti populations make this region highly vulnerable to chikungunya virus (chikv) circulation. this review describes the current dengue and chikf epidemiological situations, as well as the role of uncontrolled ae. aegypti and aedes albopictus vectors in spreading the emerging chikv. comments ...201526518229
essential oils from zanthoxylum fagara wild lime, ruta chalepensis l. and thymus vulgaris l.: composition and activity against aedes aegypti larvae.the dengue virus is transmitted by aedes aegypti. several plants are used to control this mosquito. in the present study the chemical composition of the essential oils of ruta chalepensis, zanthoxylum fagara and thymus vulgaris were analyzed, and their activities against larvae of two a. aegypti populations were evaluated. the major compounds found in t. vulgaris were thymol and -cymene at 39.8% and 30.5%, respectively, with the major components being oxygenated monoterpenes and monoterpene hyd ...201526525020
evidence for an overwintering population of aedes aegypti in capitol hill neighborhood, washington, dc.aedes aegypti is an invasive, highly anthropophilic mosquito and a major vector for dengue and chikungunya. population persistence in the continental united states is reportedly limited to southward of the average 10°c winter isotherm, which in the east, bisects alabama, mississippi, georgia, and south carolina. we report on summer collections and genotypic analyses of ae. aegypti collected in the capitol hill neighborhood in washington, dc (wdc). analysis of a 441-bp fragment of the mitochondri ...201526526922
mitochondrial dna variants help monitor the dynamics of wolbachia invasion into host populations.wolbachia is the most widespread endosymbiotic bacterium of insects and other arthropods that can rapidly invade host populations. deliberate releases of wolbachia into natural populations of the dengue fever mosquito, aedes aegypti, are used as a novel biocontrol strategy for dengue suppression. invasion of wolbachia through the host population relies on factors such as high fidelity of the endosymbiont transmission and limited immigration of uninfected individuals, but these factors can be dif ...201526531251
[estimation of aedes aegypti (l.) (diptera: culicidae) productivity in households and public spaces in a dengue endemic city in colombia].aedes aegypti is a vector for the dengue virus in colombia. its productivity can be estimated using pupal counts.201526535548
plant-mediated biosynthesis of nanoparticles as an emerging tool against mosquitoes of medical and veterinary importance: a review.mosquitoes (diptera: culicidae) are a key threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. mosquito young instars are usually targeted with organophosphates, insect growth regulators and microbial control agents. indoors residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have strong negative effects on human health and the environment. newer and safer tools have been recently implemented to enh ...201626541154
satyrization and satyrization-resistance in competitive displacements of invasive mosquito species.competitive displacements or reductions of resident populations of insects, often effected by a related species, may be caused by a variety of mechanisms. satyrization is a form of mating interference in which males of one species mate with females of another species, significantly decreasing their fitness and not generating hybrids. satyrization has been established to be the probable cause of competitive displacements of resident mosquitoes by invasive species, especially of aedes aegypti by a ...201626542083
sirna-mediated silencing of doublesex during female development of the dengue vector mosquito aedes aegypti.the development of sex-specific traits, including the female-specific ability to bite humans and vector disease, is critical for vector mosquito reproduction and pathogen transmission. doublesex (dsx), a terminal transcription factor in the sex determination pathway, is known to regulate sex-specific gene expression during development of the dengue fever vector mosquito aedes aegypti. here, the effects of developmental sirna-mediated dsx silencing were assessed in adult females. targeting of dsx ...201526544686
how important is vertical transmission of dengue viruses by mosquitoes (diptera: culicidae)?vertical transmission of dengue viruses by mosquitoes was discovered at the end of the late 1970s and has been suggested to be a means by which these viruses persist. however, it is unclear how widespread it is in nature, and its importance in the epidemiology of this disease is still debated. here, we review the literature on vertical transmission and discuss its role in dengue's epidemiology and control. we conclude that given the number of studies that failed to find evidence of vertical tran ...201626545718
linalool oxide: generalist plant based lure for mosquito disease vectors.lack of effective vaccines and therapeutics for important arboviral diseases such as rift valley fever (rvf) and dengue, necessitates continuous monitoring of vector populations for infections in them. plant-based lures as surveillance tools has the potential of targeting mosquitoes of both sexes and females of varied physiological states; yet such lures are lacking for vectors of these diseases. here, we present evidence of the effectiveness of linalool oxide (lo), a single plant-based lure pre ...201526552398
asymptomatic humans transmit dengue virus to mosquitoes.three-quarters of the estimated 390 million dengue virus (denv) infections each year are clinically inapparent. people with inapparent dengue virus infections are generally considered dead-end hosts for transmission because they do not reach sufficiently high viremia levels to infect mosquitoes. here, we show that, despite their lower average level of viremia, asymptomatic people can be infectious to mosquitoes. moreover, at a given level of viremia, denv-infected people with no detectable sympt ...201526553981
dispersal of engineered male aedes aegypti mosquitoes.aedes aegypti, the principal vector of dengue fever, have been genetically engineered for use in a sterile insect control programme. to improve our understanding of the dispersal ecology of mosquitoes and to inform appropriate release strategies of 'genetically sterile' male aedes aegypti detailed knowledge of the dispersal ability of the released insects is needed.201526554922
dengue fever as a cause of hemophagocytic lymphohistiocytosis.dengue is endemic in more than 100 countries in southeast asia, the americas, the western pacific, africa and the eastern mediterranean regions. the virus is transmitted by aedes mosquitoes. dengue disease is the most prevalent arthropod-borne viral disease in humans and is a global and national public health concern in several countries. a seasonal pattern of dengue disease is consistently observed. the highest incidences usually correspond to the period of highest rainfall and humidity, provid ...201526558245
innate antiviral immunity against dengue virus.dengue virus (denv), the most prevalent mosquito-borne viral diseases in humans worldwide, causes dengue fever, a mild form of the disease, as well as dengue hemorrhagic fever/dengue shock syndrome, a more severe form which can be life-threatening. the four serotypes of denv (denv1-4) are positive-sense, single stranded rna virus belonging to the flaviviridae family and are transmitted by aedes aegypti and aedes albopictus mosquitoes. together, they are estimated to cause almost 100 million symp ...201526559229
production of infectious dengue virus in aedes aegypti is dependent on the ubiquitin proteasome pathway.dengue virus (denv) relies on host factors to complete its life cycle in its mosquito host for subsequent transmission to humans. denv first establishes infection in the midgut of aedes aegypti and spreads to various mosquito organs for lifelong infection. curiously, studies have shown that infectious denv titers peak and decrease thereafter in the midgut despite relatively stable viral genome levels. however, the mechanisms that regulate this decoupling of infectious virion production from vira ...201526566123
surveillance of dengue vectors using spatio-temporal bayesian modeling.at present, dengue control focuses on reducing the density of the primary vector for the disease, aedes aegypti, which is the only vulnerable link in the chain of transmission. the use of new approaches for dengue entomological surveillance is extremely important, since present methods are inefficient. with this in mind, the present study seeks to analyze the spatio-temporal dynamics of a. aegypti infestation with oviposition traps, using efficient computational methods. these methods will allow ...201526566610
the impact of indoor residual spraying of deltamethrin on dengue vector populations in the peruvian amazon.dengue is an important public health problem in the amazon area of peru, resulting in significant morbidity each year. as in other areas of the world, ultra-low volume (ulv) application of insecticides is the main strategy to reduce adult populations of the dengue vector aedes aegypti, despite growing evidence of its limitations as a single control method. this study investigated the efficacy of deltamethrin s.c. applied through indoor residual spraying (irs) of dwellings in reducing a. aegypti ...201626571068
characterization and mosquitocidal potential of neem cake-synthesized silver nanoparticles: genotoxicity and impact on predation efficiency of mosquito natural enemies.mosquitoes (diptera: culicidae) serve as important vectors for a wide number of parasites and pathogens of huge medical and veterinary importance. aedes aegypti is a primary dengue vector in tropical and subtropical urban areas. there is an urgent need to develop eco-friendly mosquitocides. in this study, silver nanoparticles (agnp) were biosynthesized using neem cake, a by-product of the neem oil extraction from the seed kernels of azadirachta indica. agnp were characterized using a variety of ...201626573518
identification of a major quantitative trait locus determining resistance to the organophosphate temephos in the dengue vector mosquito aedes aegypti.organophosphate insecticides (op) have extensively been used to control mosquitoes, such as the vector aedes aegypti. unfortunately, op resistance has hampered control programs worldwide. we used quantitative trait locus (qtl) mapping to evaluate temephos resistance in two f1 intercross populations derived from crosses between a resistant ae. aegypti strain (recr) and two susceptible strains (moyod and red). a single major effect qtl was identified on chromosome 2 of both segregating populations ...201626576515
natural vertical transmission of dengue viruses in aedes aegypti in selected sites in cebu city, philippines.we attempted to determine the vertical transmission of dengue virus (denv) in aedes aegypti in selected sites in cebu city, philippines. mosquito sub-adults were collected monthly from households and the field during the wet-dry-wet season from november, 2011 to july, 2012 and were laboratory-reared to adults. viral rna extracts in mosquitoes were assayed by hemi-nested rt-pcr. results showed that 62 (36.26%; n=679) out of 171 mosquito pools (n=2,871) were denv+. the minimum infection rate (mir) ...201526611963
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