Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| gonorrhea infection in women: prevalence, effects, screening, and management. | gonorrhea is a set of clinical conditions resulting from infection with the sexually-acquired bacterial pathogen neisseria gonorrhoeae. acquisition may involve multiple mucosal sites in the lower female genital tract, including the urethra, cervix, bartholin's and skene's glands, as well as the anorectal canal, pharynx, and conjunctivae. it may spread to the upper genital tract, uterine tubes, abdominal cavity, and other systemic sites. gonorrhea is the second most commonly reported sexually-tra ... | 2011 | 21845064 |
| A new era in supraglottitis? An isolated UK case of supraglottitis secondary to Neisseria meningitidis. | We report the first UK case of supraglottitis secondary to Neisseria meningitidis. | 2011 | 21846416 |
| Emergence of a Neisseria gonorrhoeae clone showing decreased susceptibility to cefixime in England and Wales. | The third-generation cephalosporins recommended in national guidelines are amongst the last remaining effective agents for treatment of gonorrhoea. This study characterizes gonococcal isolates with decreased cefixime susceptibility from England and Wales. | 2011 | 21846672 |
| gonorrhoea surveillance, laboratory diagnosis and antimicrobial susceptibility testing of neisseria gonorrhoeae in 11 countries of the eastern part of the who european region. | quality-assured worldwide surveillance of antimicrobial resistance (amr) in neisseria gonorrhoeae is crucial for public health purposes. in the countries of the eastern part of the who european region the knowledge regarding gonococcal amr is limited, and antimicrobials of many different types, sources and quality are used for gonorrhoea treatment. this study surveyed gonorrhoea incidence, laboratory diagnosis and gonococcal amr testing in 11 independent countries of the former soviet union. the ... | 2011 | 21851423 |
| the first simultaneous study of the prevalence of neisseria gonorrhoeae and chlamydia trachomatis infections by polymerase chain reaction and standard methods. | neisseria gonorrhoeae and chlamydia trachomatis are important sexually transmissible pathogens. the aim of the present study was to determine their prevalence in symptomatic and asymptomatic people. | 2011 | 21851790 |
| Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. | Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ... | 2011 | 21853142 |
| patterns of chlamydia/gonorrhea positivity among voluntarily screened new york city public high school students. | chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) are common sexually transmitted infections that disproportionately affect adolescents. annual screening for ct for sexually active female adolescents is recommended. in 2006, new york city began conducting ct/gc education, screening, and treatment in public high schools. we examine 3-year programmatic outcomes and the relationship between sexual activity, screening, and ct/gc positivity. | 2011 | 21856516 |
| neisserial correia repeat-enclosed elements do not influence the transcription of pil genes in neisseria gonorrhoeae and neisseria meningitidis. | two human-specific neisserial pathogens, neisseria gonorrhoeae and neisseria meningitidis, require the expression of type iv pili (tfp) for initial attachment to the host during infection. however, the mechanisms controlling the assembly and functionality of tfp are poorly understood. it is known that the gonococcal pile gene, encoding the major subunit, is positively regulated by ihf, a multifunctional dna binding protein. a neisserial specific repetitive dna sequence, termed the correia repeat ... | 2011 | 21856854 |
| A Burkholderia pseudomallei macrophage infectivity potentiator-like protein has rapamycin-inhibitable peptidylprolyl isomerase activity and pleiotropic effects on virulence. | Macrophage infectivity potentiators (Mips) are a group of virulence factors encoded by pathogenic bacteria such as Legionella, Chlamydia, and Neisseria species. Mips are part of the FK506-binding protein (FKBP) family, whose members typically exhibit peptidylprolyl cis-trans isomerase (PPIase) activity which is inhibitable by the immunosuppressants FK506 and rapamycin. Here we describe the identification and characterization of BPSS1823, a Mip-like protein in the intracellular pathogen Burkholde ... | 2011 | 21859853 |
| [Nationwide antimicrobial susceptibility survey of Neisseria gonorrhoeae isolates in Japan]. | In a nationwide antimicrobial susceptibility survey of 494 Nesseria gonorrhoeae isolates collected from February 2008 to December 2009 in 3 regions of Japan, 112 (22.7%) were collected from western Japan (Kinki, Chugoku, Shikoku, and Kyushu), 277 (56.1%) from mid-eastern Japan (Kanto), and 105 (21.1%) from eastern Japan (Tokai, Hokuriku, Koushinetsu, Tohoku, and Hokkaido). Resistance to ciprofloxacin (CPFX) was 72.8%, to penicillin G (PCG) 19.8%, and to tetracycline (TC) 18.2%. Intermediate resi ... | 2011 | 21861439 |
| [A case of disseminated gonococcal infection without typical skin rash]. | Few case reports have been published on disseminated gonococcal infection in Japan. We report such a non-HIV case without typical skin rash. A 49-year-old Japanese man living in Thailand on business was seen for fever and multiple arthralgia after returning to Japan. Given the travel history, differential diagnoses included endemic viral infection such as human immunodeficiency virus (HIV), dengue fever, and chikungunya. Diagnosis was based on right-knee arthrocentesis, and synovial fluid cultur ... | 2011 | 21861441 |
| a bacterial prib with weak single-stranded dna binding activity can stimulate the dna unwinding activity of its cognate pria helicase. | bacterial dna replication restart pathways facilitate reinitiation of dna replication following disruptive encounters of a replisome with dna damage, thereby allowing complete and faithful duplication of the genome. in neisseria gonorrhoeae, the primosome proteins that catalyze dna replication restart differ from the well-studied primosome proteins of e. coli with respect to the number of proteins involved and the affinities of their physical interactions: the pria:prib interaction is weak in e. ... | 2011 | 21861872 |
| evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. | the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. | 2011 | 21864643 |
| Universal protein vaccines against Neisseria meningitidis serogroup B, Streptococcus pneumoniae and influenza. | Traditional vaccines can only be really effective when the antigenic diversity or variability of the micro-organisms in the same bacterial species or viral family is no more than marginally different, and when protection is mainly dependent from antibody-mediated immunity. One solution is to identify the antigens common to all of the strains in a species or family that are significantly capable of eliciting inactivating antibodies. A number of attempts have recently been made to develop vaccines ... | 2011 | 21865879 |
| nonhuman primate models used to study pelvic inflammatory disease caused by chlamydia trachomatis. | pelvic inflammatory disease (pid) is a global health concern that is associated with significant morbidity and is a major cause of infertility. throughout history animals have been used for anatomical studies and later as models of human disease. in particular, nonhuman primates (nhps) have permitted investigations of human disease in a biologically, physiologically, and anatomically similar system. the use of nhps as human pid models has led to a greater understanding of the primary microorgani ... | 2011 | 21869858 |
| influence of substrate modification and c-terminal truncation on the active site structure of substrate-bound heme oxygenase from neisseriae meningitidis. a 1h nmr study. | heme oxygenase (ho), from the pathogenic bacterium n. meningitidis(nmho), which secures host iron, shares many properties with mammalian hos but also exhibits some key differences. the crystal structure appears more compact, and the crystal-undetected c-terminus interacts with substrate in solution. the unique nature of substrate-protein, specifically pyrrole-i/ii-helix-2, peripheral interactions in nmho are probed by 2d (1)h nmr to reveal unique structural features controlling substrate orienta ... | 2011 | 21870860 |
| Outbreak of Neisseria meningitidis C in workers at a large food-processing plant in Brazil: challenges of controlling disease spread to the larger community. | SUMMARYAn outbreak of meningococcal disease (MD) with severe morbidity and mortality was investigated in midwestern Brazil in order to identify control measures. A MD case was defined as isolation of Neisseria meningitidis, or detection of polysaccharide antigen in a sterile site, or presence of clinical purpura fulminans, or an epidemiological link with a laboratory-confirmed case-patient, between June and August 2008. In 8 out of 16 MD cases studied, serogroup C ST103 complex was identified. F ... | 2011 | 21875453 |
| immunogenicity and safety of a haemophilus influenzae b (hib)-hepatitis b vaccine with a modified process hepatitis b component administered with concomitant pneumococcal conjugate vaccine to infants. | a hepatitis b vaccine was manufactured with a modified process (mphbv) that incorporated double the usual amount of phosphate. following a study in young adults, the mphbv was evaluated in infants in a combination hepatitis b and haemophilus influenzae b vaccine (mphbv-hib). | 2011 | 21875633 |
| role of stochastic processes in maintaining discrete strain structure in antigenically diverse pathogen populations. | many highly diverse pathogen populations appear to exist stably as discrete antigenic types despite evidence of genetic exchange. it has been shown that this may arise as a consequence of immune selection on pathogen populations, causing them to segregate permanently into discrete nonoverlapping subsets of antigenic variants to minimize competition for available hosts. however, discrete antigenic strain structure tends to break down under conditions where there are unequal numbers of allelic var ... | 2011 | 21876129 |
| adolescent female with urinary symptoms: a diagnostic challenge for the pediatrician. | the objective of this study was to determine the accuracy with which physicians diagnose sexually transmitted infections (stis) and urinary tract infections (utis) in adolescent females with urinary symptoms. secondary aims were to determine the prevalence of stis and utis in this subset of patients and to identify variables associated with a physician diagnosis of sti or uti. | 2011 | 21878833 |
| biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system. | understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ... | 2011 | 21879565 |
| Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis. | The first x-ray crystallographic structure of a CAZY family-52 glycosyltransferase, that of the membrane associated a2,3/a2,6 lipooligosaccharide sialyltransferase from Neisseria meningitidis serotype L1 (NST), has been solved to 1.95 Å resolution. The structure of NST adopts a GT-B-fold common with other glycosyltransferase (GT) families but exhibits a novel domain swap of the N-terminal 130 residues to create a functional homodimeric form not observed in any other class to date. The domain swa ... | 2011 | 21880735 |
| changes in serogroup and genotype prevalence among carried meningococci in the united kingdom during vaccine implementation. | herd immunity is important in the effectiveness of conjugate polysaccharide vaccines against encapsulated bacteria. a large multicenter study investigated the effect of meningococcal serogroup c conjugate vaccine introduction on the meningococcal population. | 2011 | 21881120 |
| successful treatment of uncomplicated gonococcal urethritis in hiv-infected patients with single-dose oral cefpodoxime. | fluoroquinolones are no longer recommended for the treatment of gonococcal infections in the united states. cephalosporins - ceftriaxone and cefixime - are the treatment of choice, as suggested by the centers for disease control and prevention (usa). there are limited data on the efficacy of cefpodoxime for the treatment of uncomplicated gonococcal infections. two cases of hiv-infected homosexual men who were successfully treated with cefpodoxime for urethritis caused by neisseria gonorrhoeae ar ... | 2010 | 21886642 |
| Age-related immunity to meningococcal serogroup C vaccination: an increase in the persistence of IgG2 correlates with a decrease in the avidity of IgG. | All children and adolescents between 1 and 19 years of age in The Netherlands received a single meningococcal serogroup C conjugate (MenCC) vaccine in 2002. During follow-up 4-5 years later, the persistence of MenC polysaccharide-specific IgG was found to be dependent on age of vaccination with higher IgG levels in the oldest immunized age categories. | 2011 | 21887261 |
| Quantification of polyphenols during retting and characterization of bacteria from the Kadinamkulam Backwaters, Kerala. | The retting environment which provides a competitive niche for specialized microbes is speculated to harbour a variety of microbes with high biodegradation potential. In this context, an effort has been made to isolate and identify bacterial species having high tolerance to phenol In vitro. Maximum polyphenol (1.897 mg l(-1)) as observed during the initial period of retting, which decreased as retting proceeded. Based on biochemical characterization, the isolated bacterial strains were identifie ... | 2011 | 21888245 |
| genotyping of two neisseria gonorrhoeae fluroquinolone-resistant strains in the brazilian amazon region. | we report two ciprofloxacin and ofloxacin-resistant neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the alfredo da matta foundation (manaus, state of amazonas, brazil). the gonococci displayed minimal inhibitory concentrations (> 32.00 µg/ml) and three mutations in the quinolone resistance-determining region (s91f and d95g in gyra and s87r in parc). both isolates were genotyped using n. gonorr ... | 2011 | 21894387 |
| a case of meningitis due to neisseria subflava after ventriculostomy. | surgical site infection (ssi) is an emerging problem in all fields of surgery, as well as in neurosurgery, with numerous strategies against ssi initiated in many countries. in this report, we describe a case of ssi caused by neisseria subflava a month after ventriculostomy placement for acute hydrocephalus. a 53-year old man was referred to our hospital, and was diagnosed with a thalamic hemorrhage with ventricular rupture. bilateral ventriculostomies were performed on the day of presentation. a ... | 2011 | 21894453 |
| Early microbial succession in redeveloping dental biofilms in periodontal health and disease. | Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ... | 2011 | 21895662 |
| identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack faba. | transcriptome analysis of the facultative anaerobe, neisseria gonorrhoeae, revealed that many genes of unknown function were induced under anaerobic conditions. mutation of one such gene, ngo1024, encoding a protein belonging to the 2-nitropropane dioxygenase-like superfamily of proteins, was found to result in an inability of gonococci to grow anaerobically. anaerobic growth of an ng1024 mutant was restored upon supplementation with unsaturated fatty acids (ufa), but not with the saturated fatt ... | 2011 | 21895795 |
| [Male urethral duplication infection: experience with 9 cases]. | To study the clinical characteristics of male urethral duplication infection and offer some guidelines for the diagnosis and treatment of the disease. | 2011 | 21898997 |
| Factors associated with HIV among female sex workers in a high HIV prevalent state of India. | Abstract The study was carried out to assess the factors associated with HIV seropositivity among female sex workers (FSWs) in Dimapur, Nagaland, a high HIV prevalence state of India. A total of 426 FSWs were recruited into the study using respondent driven sampling (RDS). Data on demographic characteristics, sexual and injecting risk behaviours were collected from them and were tested for HIV, Syphilis, Neisseria gonorrhoeae and Chlamydia trachomatis. RDS-weighted univariate and multivariate lo ... | 2011 | 21902571 |
| the first use of an investigational multicomponent meningococcal serogroup b vaccine (4cmenb) in humans. | neisseria meningitidis serogroup b is a well-recognized cause of bacterial meningitis and sepsis for which no broadly protective vaccine exists. whole genome sequencing was used to identify three antigens: factor h binding protein (fhbp), neisserial adhesin a (nada), and neisseria heparin binding antigen (nhba) for an investigational vaccine candidate (rmenb). this was the first trial of an investigational multicomponent meningococcal serogroup b vaccine (4cmenb), containing rmenb and outer memb ... | 2011 | 21904120 |
| the challenge of developing universal vaccines. | antigenic variability of immunodominant antigens is a common mechanism used by pathogens to escape the immune response. frequently, the proposed solution is a universal vaccine based on conserved antigens present on all strains of the pathogen. indeed, a lot of progress has been made in the development of vaccines that induce broad immune responses. however, truly universal vaccines are not easy to produce and still face many challenges, mostly because in those pathogens that use antigenic varia ... | 2011 | 21904572 |
| carriage of neisseria meningitidis in europe: a review of studies undertaken in the region. | meningococcal conjugate vaccines induce herd protection by preventing nasopharyngeal meningococcal acquisition, which is a prerequisite for invasive disease. thus, meningococcal carriage epidemiology is important in understanding relationships between carriage and disease. a literature search traced information on meningococcal carriage in 27 eu countries. meningococcal carriage prevalence differed within and between countries, varying across age groups, serogroup distribution and over time. car ... | 2011 | 21905785 |
| cleavage of von willebrand factor by granzyme m destroys its factor viii binding capacity. | von willebrand factor (vwf) is a pro-hemostatic multimeric plasma protein that promotes platelet aggregation and stabilizes coagulation factor viii (fviii) in plasma. the metalloproteinase adamts13 regulates the platelet aggregation function of vwf via proteolysis. severe deficiency of adamts13 is associated with thrombotic thrombocytopenic purpura, but does not always correlate with its clinical course. therefore, other proteases could also be important in regulating vwf activity. in the presen ... | 2011 | 21909423 |
| meningococcal factor h binding proteins in epidemic strains from africa: implications for vaccine development. | factor h binding protein (fhbp) is an important antigen for vaccines against meningococcal serogroup b disease. the protein binds human factor h (fh), which enables the bacteria to resist serum bactericidal activity. little is known about the vaccine-potential of fhbp for control of meningococcal epidemics in africa, which typically are caused by non-group b strains. | 2011 | 21909444 |
| induction of immune responses by purified outer membrane protein complexes from neisseria meningitidis. | a broad-spectrum vaccine against disease caused by serogroup b of neisseria meningitidis is still a challenge due to antigenic variability. in the present study outer membrane protein complexes and their components were analysed using non-denaturing 2d electrophoresis and identified using lc/ms-ms and maldi-tof. outer membrane protein complexes were purified from both the wild-type strain h44/76 and their knock-out mutants lacking pora, porb, rmpm or feta. the immune responses elicited by the wh ... | 2011 | 21911024 |
| introducing shear stress in the study of bacterial adhesion. | during bacterial infections a sequence of interactions occur between the pathogen and its host. bacterial adhesion to the host cell surface is often the initial and determining step of the pathogenesis. although experimentally adhesion is mostly studied in static conditions adhesion actually takes place in the presence of flowing liquid. first encounters between bacteria and their host often occur at the mucosal level, mouth, lung, gut, eye, etc. where mucus flows along the surface of epithelial ... | 2011 | 21912368 |
| [anti-cytokine activity of microorganisms]. | development of a method of determination of anti-cytokine activity (aca) of microorganisms, study of the prevalence and intensity of aca to pro- and anti-inflammatory cytokines in pathogenic and opportunistic bacteria. | 2011 | 21913393 |
| [meningitis and encephalitis in poland in 2009]. | in poland, 2 517 cases of neuroinfections were reported in 2009, of which 865 had bacterial aetiology, 1 244--viral aetiology, and 408-- other or unknown origin. the etiological agent was determined in 493 (57%) cases of bacterial neuroinfections. among them neisseria meningitidis was found in 190 cases, haemophilus influenzae type b (hib) in 13 cases and streptococcus pneumoniae in 163 cases. an increasing trend in pneumococcal infections incidence has been observed since 2005, and a substantia ... | 2011 | 21913462 |
| estimating the prevalence of gonococcal resistance to antimicrobials in england and wales. | gonorrhoea can rapidly develop resistance to antimicrobials and treatment options are becoming depleted. treatment guidelines require robust estimates of the prevalence of resistance but sentinel surveillance systems may not be representative nationally. | 2011 | 21917698 |
| Neisseria gonorrhoeae NspA induces specific bactericidal and opsonic antibodies in mice. | Neisseria gonorrhoeae surface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene from N. gonorrhoeae strain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) in ... | 2011 | 21918113 |
| Neisseria meningitidis B vaccines. | Invasive infections caused by Neisseria meningitidis are a serious public health problem worldwide and have a heavy economic impact. The incidence of invasive disease due to Neisseria meningitidis is highly variable according to geographical area and serogroup distribution. Since the introduction of vaccination programs with conjugated vaccine C in children and adolescents, most cases of invasive meningococcal disease in developed countries have been caused by meningococcus B. It is important to ... | 2011 | 21919622 |
| Disseminated gonococcal infection in Japan: a case report and literature review. | Disseminated gonococcal infection (DGI) is quite a rare condition, especially in the Japanese population; only 10 cases have been reported in case notes and minutes. We describe a man in which Neisseria gonorrhoeae was suspected to have infected the patient through his pharynx. He developed chills, fever, tonsillitis, papules, tenosynovitis and migratory polyarthralgia without genitourinary symptoms. After conducting a literature review, we suggest that being male is a possible risk factor and t ... | 2011 | 21921393 |
| the role of bradykinin and the effect of the bradykinin receptor antagonist icatibant in porcine sepsis. | bradykinin (bk) is regarded as an important mediator of edema, shock, and inflammation during sepsis. in this study, we evaluated the contribution of bk in porcine sepsis by blocking bk and by measuring the stable bk metabolite, bk1-5, using anesthetized pigs. the effect of bk alone, the efficacy of icatibant to block this effect, and the recovery of bk measured as plasma bk1-5 were first investigated. purified bk injected intravenously induced an abrupt fall in blood pressure, which was complet ... | 2011 | 21921836 |
| From agonist to antagonist: structure and dynamics of innate immune glycoprotein MD-2 upon recognition of variably acylated bacterial endotoxins. | The human immune response to an infection by Gram-negative bacteria involves detection of lipopolysaccharides (LPS), also known as endotoxins, which comprise the bacterial outer cell wall. Distinct from mammalian glycolipid structures, LPS have a conserved chemical pattern that is recognized by the pattern recognition receptor complex formed by myeloid differentiation protein 2 (MD-2) and toll-like receptor 4 (TLR4). A remarkable immune-mediated structure-toxicity relationship has been defined t ... | 2011 | 21924775 |
| evaluation of the siemens versant® ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae. | the siemens versant kpcr system is an automated system which combines extraction of nucleic acids from 96 samples with subsequent real-time pcr. the versant ct/gc dna 1.0 (kpcr) assay detects chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) in a multiplex real-time pcr on this system. we compared this assay with the bd probete™ et system (pt) and the roche cobas amplicor (ca). three different sets of samples were tested in the kpcr: pt pre-treated samples, prospectively collected urine ... | 2011 | 21925547 |
| cutaneous gonococcal abscess of the abdomen in a child. | virtually all pediatric cases of neisseria gonorrhoeae originate from contact with an infected adult. a cutaneous abscess caused by n. gonorrhoeae in a child is extremely rare, especially outside the genital area. we report a case of a 22-month-old boy with a gonococcal cutaneous abscess on the abdominal wall and suggest that n. gonorrhoeae should be included in the differential diagnosis of skin and soft tissue infections in children. | 2011 | 21926887 |
| Distribution of serogroups and sequence types in disease-associated and carrier strains of Neisseria meningitidis isolated in China between 2003 and 2008. | SUMMARYGiven the unpredictability of Neisseria meningitidis outbreaks and the increased prevalence of serogroup C strains following the introduction of serogroup A-based vaccines, we conducted an analysis of serogroups and sequence types (STs) in disease-associated and carrier N. meningitidis isolates that have emerged in China since 2003. We used multilocus sequence-typing techniques to investigate 371 N. meningitidis strains isolated from patients with meningitis and healthy carriers. Two line ... | 2011 | 21929839 |
| neisseria base: a comparative genomics database for neisseria meningitidis. | neisseria meningitidis is an important pathogen, causing life-threatening diseases including meningitis, septicemia and in some cases pneumonia. genomic studies hold great promise for n. meningitidis research, but substantial database resources are needed to deal with the wealth of information that comes with completely sequenced and annotated genomes. to address this need, we developed neisseria base (nbase), a comparative genomics database and genome browser that houses and displays publicly a ... | 2011 | 21930505 |
| gonorrhea: what goes around comes around. | 2011 | 21930743 | |
| fbpa - a bacterial transferrin with more to offer. | background: gram negative bacteria require iron for growth and virulence. it has been shown that certain pathogenic bacteria such as neisseria gonorrhoeae possess a periplasmic protein called ferric binding protein (fbpa), which is a node in the transport of iron from the cell exterior to the cytosol. scope of review: the relevant literature is reviewed which establishes the molecular mechanism of fbpa mediated iron transport across the periplasm to the inner membrane. major conclusions: here we ... | 2011 | 21933698 |
| a novel mechanism of high-level, broad-spectrum antibiotic resistance caused by a single base pair change in neisseria gonorrhoeae. | the mtrc-mtrd-mtre multidrug efflux pump of neisseria gonorrhoeae confers resistance to a diverse array of antimicrobial agents by transporting these toxic compounds out of the gonococcus. frequently in gonococcal strains, the expression of the mtrcde operon is differentially regulated by both a repressor, mtrr, and an activator, mtra. the mtrr gene lies 250 bp upstream of and is transcribed divergently from the mtrcde operon. previous research has shown that mutations in the mtrr coding region ... | 2011 | 21933917 |
| Trends in antimicrobial resistance in Neisseria gonorrhoeae isolated in Canada: 2000-2009. | Canada conducts surveillance of penicillin, tetracycline, erythromycin, spectinomycin, ciprofloxacin, cefixime, and ceftriaxone susceptibilities in Neisseria gonorrhoeae isolates to support development of national treatment guidelines for sexually transmitted infections. | 2011 | 21934558 |
| Sustainable antimicrobial surveillance programs essential for controlling Neisseria gonorrhoeae superbug. | 2011 | 21934559 | |
| testing men who have sex with men for urethral infection with chlamydia trachomatis and neisseria gonorrhoeae is only half the job, and we need the right tools. | 2011 | 21934566 | |
| chlamydia trachomatis prevalence and risk behaviors in parturient women aged 15 to 24 in brazil. | chlamydia trachomatis (ct) is a sexually transmitted infection having repercussions on reproductive health and impact on the foetus. our goal was to estimate the prevalence of and risk factors for ct in young parturient women in brazil. | 2011 | 21934572 |
| epidemiology of invasive meningococcal disease in austria 2010. | neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. thus, monitoring the epidemiology of invasive meningococcal disease (imd) is an important public health measure. the national reference centre for meningococci at the austrian agency for health and food safety is operating the laboratory-based surveillance database. | 2011 | 21935643 |
| Protective role of Toll-like receptor 4 in experimental gonococcal infection of female mice. | Neisseria gonorrhoeae is a common bacterial sexually transmitted infection. Like all Gram-negative bacteria, the outer membrane of the gonococcus is rich in endotoxin, a known ligand for Toll-like receptor (TLR)4. However, the role of endotoxin and that of its cognate receptor TLR4 in the mucosal response to acute gonococcal infection in the genital tract of women is unclear. To test this, we examined the course of infection after vaginal inoculation of N. gonorrhoeae in mice carrying the Lps(d) ... | 2012 | 21937985 |
| increasing trend of resistance to penicillin, tetracycline, and fluoroquinolone resistance in neisseria gonorrhoeae from pakistan (1992-2009). | emergence and spread of drug resistant neisseria gonorrhoeae is global concern. we evaluated trends of antimicrobial resistance in neisseria gonorrhoeae over years 1992-2009 in pakistan. resistance rates were compared between years (2007-2009) and (1992-2006). antimicrobial susceptibility testing was performed and interpreted according to clinical laboratory standards institute (clsi) criteria using the disk diffusion methodology against penicillin, ceftriaxone, tetracycline and ofloxacin. addit ... | 2011 | 21941568 |
| An extended ß7a7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. | The enzyme 3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the reaction between phosphoenolpyruvate and arabinose 5-phosphate (A5P) in the first committed step in the biosynthetic pathway for the formation of 3-deoxy-D-manno-octulosonate, an important component in the cell wall of Gram-negative bacteria. KDO8P synthase is evolutionarily related to the first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) synthase, which uses erythrose 4-ph ... | 2011 | 21942786 |
| Innate immune mediator profiles and their regulation in a novel polarized immortalized epithelial cell model derived from human endocervix. | The endocervix in the female reproductive tract (FRT) is susceptible to sexually transmitted pathogens such as Chlamydia trachomatis and Neisseria gonorrhoeae. Endocervical epithelial cells in vivo make innate immune mediators that likely aid in the protection from these pathogens. In vitro studies to investigate the innate epithelial cell immune response to endocervical pathogens have been hindered by the paucity of human endocervix-derived epithelial cell lines that display the differentiation ... | 2011 | 21943934 |
| comparative long-term adverse effects elicited by invasive group b and c meningococcal infections. | given the identity between neisseria meningitidis serogroup b (menb) capsular polysaccharide (polysialic acid; psa) and psa found on neural cell adhesion molecules, it has been proposed that infection with menb or vaccination with psa may be associated with subsequent autoimmune or neurological disease. | 2011 | 21946191 |
| the iron-repressed, arac-like regulator mper activates expression of feta in neisseria gonorrhoeae. | neisseria gonorrhoeae is an obligate human pathogen that causes the common sexually transmitted infection gonorrhea. gonococcal infections cause significant morbidity, particularly among women, as the organism ascends to the upper reproductive tract, resulting in pelvic inflammatory disease, ectopic pregnancy, and infertility. in the last few years, antibiotic resistance rates have risen dramatically, leading to severe restriction of treatment options for gonococcal disease. gonococcal infection ... | 2011 | 21947770 |
| pathogenic neisseria hitchhike on the uropod of human neutrophils. | polymorphonuclear neutrophils (pmns) are important components of the human innate immune system and are rapidly recruited at the site of bacterial infection. despite the effective phagocytic activity of pmns, neisseria gonorrhoeae infections are characterized by high survival within pmns. we reveal a novel type iv pilus-mediated adherence of pathogenic neisseria to the uropod (the rear) of polarized pmns. the direct pilus-uropod interaction was visualized by scanning electron microscopy and tota ... | 2011 | 21949708 |
| The soluble recombinant Neisseria meningitidis adhesin NadA(?351-405) stimulates human monocytes by binding to extracellular Hsp90. | The adhesin NadA favors cell adhesion/invasion by hypervirulent Neisseria meningitidis B (MenB). Its recombinant form NadA(?351-405,) devoid of the outer membrane domain, is an immunogenic candidate for an anti-MenB vaccine able to stimulate monocytes, macrophages and dendritic cells. In this study we investigated the molecular mechanism of NadA(?351-405) cellular effects in monocytes. We show that NadA(?351-405) (against which we obtained polyclonal antibodies in rabbits), binds to hsp90, but n ... | 2011 | 21949862 |
| evaluation of chlamydia trachomatis and neisseria gonorrhoeae detection in urine, endocervical, and vaginal specimens by a multiplexed isothermal thermophilic helicase-dependent amplification (thda) assay. | we have developed a new research assay that combines sequence-specific sample preparation and isothermal amplification for the detection of chlamydia trachomatis and neisseria gonorrhoeae infections. the assay targets both the omp gene and the cryptic plasmid of c. trachomatis and the multicopy opa gene of n. gonorrhoeae, which are amplified and detected in a single reaction. we evaluated the ability of the assay to detect c. trachomatis and n. gonorrhoeae infections in first-catch urine, swab, ... | 2011 | 21956990 |
| Differing Neisseria gonorrhoeae Bacterial Loads in the Pharynx and Rectum in Men Who Have Sex with Men: Implications for Gonococcal Detection, Transmission, and Control. | The bacterial loads for gonococcal infections of the pharynx and rectum were determined among men with male sexual partners. The median bacterial load for rectal infections (18,960 copies/swab) was significantly higher than the load for pharyngeal infections (2,100 copies/swab; P = 0.001). Bacterial loads among men with symptomatic proctitis were strikingly high (median, 278,000 copies/swab). | 2011 | 21956992 |
| implementation and impact of a meningococcal c conjugate vaccination program in 13- to 25-year-old individuals in galicia, spain. | background: in response to increased case numbers of meningococcal group c disease, catch-up vaccination strategies have been shown to be successful. this paper describes the results of a repeat vaccination program in galicia, spain, and the strategy used for it. methods and results: three vaccination waves were performed: first, in 1996/1997 with a meningococcal group a and c polysaccharide vaccine in individuals aged 18 months to 19 years; second, in 2000 with a conjugate serogroup c polysacc ... | 2011 | 21957332 |
| phenotypic and genotypic properties of neisseria gonorrhoeae isolates in norway in 2009: antimicrobial resistance warrants an immediate change in national management guidelines. | despite rapidly diminishing treatment options for neisseria gonorrhoeae and high levels of ciprofloxacin resistance worldwide, norwegian guidelines still recommend ciprofloxacin as empirical treatment for gonorrhea. the present study aimed to characterize phenotypical and genotypical properties of n. gonorrhoeae isolates in norway in 2009. all viable n. gonorrhoeae isolates (n = 114) from six university hospitals in norway (2009) were collected, representing 42% of all notified gonorrhea cases. ... | 2011 | 21960034 |
| acute bacterial meningitis in infants and children: epidemiology and management. | acute bacterial meningitis (abm) continues to be associated with high mortality and morbidity, despite advances in antimicrobial therapy. the causative organism varies with age, immune function, immunization status, and geographic region, and empiric therapy for meningitis is based on these factors. haemophilus influenzae type b (hib), streptococcus pneumoniae, and neisseria meningitidis cause the majority of cases of abm.disease epidemiology is changing rapidly due to immunization practices and ... | 2011 | 21960054 |
| pre-hospital antibiotics for meningococcal disease remains low. | 2011 | 21964019 | |
| Structure of the C-terminal Domain of Neisseria Heparin Binding Antigen (NHBA), One of the Main Antigens of a Novel Vaccine against Neisseria meningitidis. | Neisseria heparin binding antigen (NHBA), also known as GNA2132 (genome-derived Neisseria antigen 2132), is a surface-exposed lipoprotein from Neisseria meningitidis that was originally identified by reverse vaccinology. It is one the three main antigens of a multicomponent vaccine against serogroup B meningitis (4CMenB), which has just completed phase III clinical trials in infants. In contrast to the other two main vaccine components, little is known about the origin of the immunogenicity of t ... | 2011 | 21965688 |
| meningococcal purpura fulminans in children: i. initial orthopedic management. | purpura fulminans is a rare and extremely severe infection, mostly due to neisseria meningitidis frequently causing early orthopedic lesions. few studies have reported on the initial surgical management of acute purpura fulminans. the aim of this study is to look at the predictive factors in orthopedic outcome in light of the initial surgical management in children surviving initial resuscitation. | 2010 | 21966303 |
| meningococcal purpura fulminans in children. ii: late orthopedic sequelae management. | purpura fulminans is a rare and extremely severe infection, mostly due to neisseria meningitidis. nineteen patients were followed up immediately after the initial multivisceral failure in order to diagnose late-onset orthopedic sequelae. we report our experience with these 19 patients, in light of our medical follow-up protocol and surgical management. | 2010 | 21966304 |
| cefixime and ceftriaxone susceptibility of neisseria gonorrhoeae in italy from 2006 to 2010. | clin microbiol infect abstract: neisseria gonorrhoeae resistance to cephalosporins, the currently recommended treatment, and treatment failures with cefixime have been reported worldwide. the purposes of the present study were (i) to examine the susceptibility of n. gonorrhoeae isolates isolated in italy from 2006 through 2010 to cefixime (n = 293) taking into account both european committee on antimicrobial susceptibility testing (eucast) and clinical and laboratory standards institute (clsi) c ... | 2011 | 21966997 |
| pasteurella multocida cmp-sialic acid synthetase and mutants of neisseria meningitidis cmp-sialic acid synthetase with improved substrate promiscuity. | cytidine 5'-monophosphate (cmp)-sialic acid synthetases (csss) catalyze the formation of cmp-sialic acid from ctp and sialic acid, a key step for sialyltransferase-catalyzed biosynthesis of sialic acid-containing oligosaccharides and glycoconjugates. more than 50 different sialic acid forms have been identified in nature. to facilitate the enzymatic synthesis of sialosides with diverse naturally occurring sialic acid forms and their non-natural derivatives, cmp-sialic acid synthetases with promi ... | 2011 | 21968653 |
| elevated soluble urokinase receptor values in csf, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome. | the aim of the present study was to evaluate the potential role of cerebrospinal fluid soluble urokinase receptor (supar) level, infection and age as risk factors for fatal outcome in patients suspected of having meningitis and/or bacteraemia on admission to hospital. a total of 545 cerebrospinal fluid samples from patients with clinically suspected meningitis were sent to the hellenic national meningitis reference laboratory. ten of 545 (1.83%) patients died. analysis by receiver operating char ... | 2011 | 21971819 |
| antimicrobial resistance in neisseria gonorrhoeae in the uk: surveillance and management. | successful antimicrobial therapy is fundamental to the public health control of gonorrhea, in the absence of a protective immune response. neisseria gonorrhoeae, the causative agent, has presented a constant challenge for the provision of such therapy as it has demonstrated the ability to become resistant to successive highly active agents chosen for first-line treatment. acquisition of plasmids from other bacteria and long-term use of a single agent has selected both single step high-level and ... | 2011 | 21973299 |
| Neisseria sicca endocarditis requiring mitral valve replacement in a previously healthy adolescent. | Infective endocarditis due to Neisseria sicca, a normal inhabitant of the upper respiratory tract, is rarely reported but associated with embolic phenomena and large vegetations often requiring surgical intervention. We report a previously healthy 12-year-old girl who presented with prolonged fever and altered mental status. The patient developed rapidly progressive respiratory insufficiency and cardiovascular instability, and echocardiography demonstrated a large vegetation on the mitral valve. ... | 2011 | 21975499 |
| crystal structure of a bacterial homologue of the bile acid sodium symporter asbt. | high cholesterol levels greatly increase the risk of cardiovascular disease. about 50 per cent of cholesterol is eliminated from the body by its conversion into bile acids. however, bile acids released from the bile duct are constantly recycled, being reabsorbed in the intestine by the apical sodium-dependent bile acid transporter (asbt, also known as slc10a2). it has been shown in animal models that plasma cholesterol levels are considerably lowered by specific inhibitors of asbt, and asbt is t ... | 2011 | 21976025 |
| molecular characterization of quinolone-resistant neisseria gonorrhoeae isolates from brazil. | despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from brazilian locations. in the present study, 25 quinolone-resistant neisseria gonorrhoeae (qrng) strains isolated in rio de janeiro, brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyra and parc genes. they represented 16.5% of the n. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. a trend for increasing re ... | 2011 | 21976763 |
| viewpoint: gentamicin for treatment of gonococcal urethritis in malawi. | 2010 | 21977848 | |
| A novel metal transporter mediating manganese export (MntX) regulates the Mn to Fe intracellular ratio and Neisseria meningitidis virulence. | Neisseria meningitidis (Nm) and N. gonorrhoeae (Ng) are adapted to different environments within their human host. If the basis of this difference has not yet been fully understood, previous studies (including our own data) have reported that, unlike Ng, Nm tolerates high manganese concentrations. As transition metals are essential regulators of cell growth and host pathogen interactions, we aimed to address mechanisms of Nm Mn²? tolerance and its pathogenic consequences. Using bioinformatics, g ... | 2011 | 21980287 |
| structural bioinformatics of neisseria meningitidis ld-carboxypeptidase: implications for substrate binding and specificity. | neisseria meningitidis, a gram negative bacterium, is the leading cause of bacterial meningitis and severe sepsis. neisseria meningitidis genome contains 2,160 predicted coding regions including 1,000 hypothetical genes. re-annotation of n. meningitidis hypothetical proteins identified nine putative peptidases. among them, the nmb1620 protein was annotated as ld-carboxypeptidase involved in peptidoglycan recycling. structural bioinformatics studies of nmb1620 protein using homology modeling and ... | 2011 | 21983752 |
| Deletion of one nucleotide within the homonucleotide tract present in the hsdS gene alters the DNA sequence specificity of type I restriction-modification system NgoAV. | As a result of a frameshift mutation, the hsdS locus of the NgoAV type IC restriction and modification (RM) system comprises two genes, hsdS(NgoAV1) and hsdS(NgoAV2). The specificity subunit, HsdS(NgoAV), the product of the hsdS(NgoAV1) gene, is a naturally truncated form of an archetypal specificity subunit (208 N-terminal amino acids instead of 410). The presence of a homonucleotide tract of seven guanines (poly[G]) at the 3' end of the hsdS(NgoAV1) gene makes the NgoAV system a strong candida ... | 2011 | 21984785 |
| Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. | Please cite this paper as: Allard et al. (2011) Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00297.x. Background Shared seasonal patterns, such as between influenza and some respiratory bacterial infections, can create associations between phenomena not causally related. Objectives To estimate the association of influenza with subsequent bacterial infections after ... | 2011 | 21985083 |
| establishment of a human ceacam1 transgenic mouse model for the study of gonococcal infections. | neisseria gonorrhoeae is the causative microorganism for the sexually transmitted disease (std) gonorrhea and humans are its only natural host. an animal model would be a useful tool for gonorrhea research, therefore we developed the hceacam1 transgenic mice, using an eukaryotic expression vector, pcdpcam1-gi. this construct was microinjected into the zygotes of c57bl/6 mice and 22 f0 generation transgenic mice were obtained. four (lines 50, 53, 54, and 59) of the f0 generation were found to car ... | 2011 | 21986029 |
| A functional microsatellite of the macrophage migration inhibitory factor gene associated with meningococcal disease. | Macrophage migration inhibitory factor (MIF) is an abundantly expressed proinflammatory cytokine playing a critical role in innate immunity and sepsis and other inflammatory diseases. We examined whether functional MIF gene polymorphisms (-794 CATT(5-8) microsatellite and -173 G/C SNP) were associated with the occurrence and outcome of meningococcal disease in children. The CATT(5) allele was associated with the probability of death predicted by the Pediatric Index of Mortality 2 (P=0.001), whic ... | 2011 | 21990375 |
| Communicable diseases prioritized for surveillance and epidemiological research: results of a standardized prioritization procedure in Germany, 2011. | To establish strategic priorities for the German national public health institute (RKI) and guide the institute's mid-term strategic decisions, we prioritized infectious pathogens in accordance with their importance for national surveillance and epidemiological research. | 2011 | 21991334 |
| conjugate meningococcal vaccines development: gsk biologicals experience. | meningococcal diseases are serious threats to global health, and new vaccines specifically tailored to meet the age-related needs of various geographical areas are required. this paper focuses on the meningococcal conjugate vaccines developed by gsk biologicals. two combined conjugate vaccines were developed to help protect infants and young children in countries where the incidence of meningococcal serogroup c or serogroup c and y disease is important: hib-menc-tt vaccine, which offers protecti ... | 2011 | 21991444 |
| european efforts to harmonize typing of meningococci. | neisseria meningitidis causes life-threatening disease in infants, toddlers, and adolescents. besides representative case notification, public health management of the disease requires bacterial typing information. european reference laboratories and state epidemiologists in collaboration with european institutions have driven forward the harmonization of typing by rigorously adopting dna sequence typing and using common reference databases. external quality assessment has been provided by supra ... | 2011 | 21992745 |
| Evaluation of self-collected versus clinician-collected swabs for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae pharyngeal infection among men who have sex with men. | We evaluated self-sampling to detect pharyngeal Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infection among men who have sex with men attending a San Francisco STD clinic. The prevalence of pharyngeal NG and CT infection was 6.7% (32/480) and 1.3% (6/480), respectively. The percent agreement between self-collected and clinician-collected NG and CT specimens using nucleic acid amplification testing was 96.6% with a ? of 0.766 (95% confidence interval: 0.653-0.879) and 99.4% with a ? ... | 2011 | 21992980 |
| characterization of profile of multidrug-resistant neisseria gonorrhoeae using old and new definitions in india over a decade: 2000-2009. | the burden of "multidrug-resistant neisseria gonorrhoeae" (mdr-ng) was high considering the old definition (26.0%). according to the new definitions, no strain was mdr or extensively drug-resistant. the emergence of resistance to ceftriaxone and cefixime will lead to detection of mdr-ng and may be extensively drug-resistant ng, even according to the new definition. | 2011 | 21992984 |
| Trends in antimicrobial resistance of Neisseria gonorrhoeae isolated from Korean patients from 2000 to 2006. | The recent emergence and spread of antimicrobial-resistant Neisseria gonorrhoeae has compromised treatment and control of gonorrhea. We determined recent trends in antimicrobial susceptibility of the isolates, analyzed recent use of antigonococcal agents, and investigated the relationship between fluoroquinolone nonsusceptibility and amino acid substitutions within the fluoroquinolone resistance-determining regions in Korea. | 2011 | 21992988 |
| neisseria meningitidis: biology, microbiology, and epidemiology. | neisseria meningitidis (the meningococcus) causes significant morbidity and mortality in children and young adults worldwide through epidemic or sporadic meningitis and/or septicemia. in this review, we describe the biology, microbiology, and epidemiology of this exclusive human pathogen. n.meningitidis is a fastidious, encapsulated, aerobic gram-negative diplococcus. colonies are positive by the oxidase test and most strains utilize maltose. the phenotypic classification of meningococci, based ... | 2012 | 21993636 |
| classification and pathogenesis of meningococcal infections. | the clinical symptoms induced by neisseria meningitidis reflect compartmentalized intravascular and intracranial bacterial growth and inflammation. in this chapter, we describe a classification system for meningococcal disease based on the nature of the clinical symptoms. meningococci invade the subarachnoid space and cause meningitis in as many as 50-70% of patients. the bacteremic phase is moderate in patients with meningitis and mild systemic meningococcemia but graded high in patients with s ... | 2012 | 21993637 |
| detection of neisseria meningitidis in cerebrospinal fluid using a multiplex pcr and the luminex detection technology. | rapid clinical and laboratory diagnoses are the foundation for a successful management of serious infections with neisseria meningitidis. a species-specific multiplex polymerase chain reaction (pcr) coupled with fluidic microarrays using microbeads (the luminex xmap™ technology) can detect pathogens most frequently found in the cerebrospinal fluid of patients. the luminex suspension array system uniquely combines flow cytometry, microspheres, laser technology, digital signal processing, and trad ... | 2012 | 21993638 |
| generating knock-out and complementation strains of neisseria meningitidis. | the human-restricted pathogens neisseria meningitidis and neisseria gonorrhoeae are naturally competent for dna uptake. this trait has been exploited extensively for genetic manipulation of these bacteria in the laboratory. most transformation protocols were developed for n. gonorrhoeae, but appear to work also for n. meningitidis. in this chapter, we describe a number of protocols for genetic manipulation of n. meningitidis. specifically, we describe how to (1) obtain knock-out mutants containi ... | 2012 | 21993639 |