Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| peroxidase induced oligo-tyrosine cross-links during polymerization of α-lactalbumin. | horseradish peroxidase (hrp) induced cross-linking of proteins has been reported to proceed through formation of di-tyrosine cross-links. in the case of low molar mass phenolic substrates, the enzymatic oxidation is reported to lead to polymerization of the phenols. the aim of this work was to investigate if during oxidative cross-linking of proteins oligo-tyrosine cross-links are formed in addition to dityrosine. to this end, α-lactalbumin (α-la) was cross-linked using horseradish peroxidase (h ... | 2015 | 26282909 |
| optimized enzymatic colorimetric assay for determination of hydrogen peroxide (h2o2) scavenging activity of plant extracts. | the classical method to determine hydrogen peroxide (h2o2) scavenging activity of plant extracts is evaluated by measuring the disappearance of h2o2 at a wavelength of 230 nm. since this method suffers from the interference of phenolics having strong absorption in the uv region, a simple and rapid colorimetric assay was developed where plant extracts are introduced to h2o2, phenol and 4-aminoantipyrine reaction system in the presence of horseradish peroxidase (hrp). this reaction yields a quinon ... | 2015 | 26285798 |
| intelligent dna machine for the ultrasensitive colorimetric detection of nucleic acids. | as dna is employed to serve as a smart building block, an increasing interest has been devoted to the development of different dna-based machines for the specific purpose, for example, the exploration of inter- or intramolecular interaction. in the current contribution, we developed an intelligent dna machine and its operation can be designed to execute the ultrasensitive colorimetric detection of target nucleic acids. the dna machine consists of a hairpin probe (hp) and an assistant template (a ... | 2016 | 26291961 |
| enzyme-triggered formation of enzyme-tyramine concatamers on nanogold-functionalized dendrimer for impedimetric detection of hg(ii) with sensitivity enhancement. | a new impedimetric sensing strategy based on enzyme-triggered formation of enzyme-tyramine concatamers on the nanogold-functionalized poly(amidoamine) (pamam) dendrimer was designed for sensitive detection of mercury(ii) (hg(2+)) ion, coupling with enzymatic biocatalytic precipitation towards 4-choloro-1-naphthol (4-cn) on thymine (t)-rich single-stranded dna1-modified electrode. initially, nanogold-decorated pamam dendrimer (aunp-pamam) was synthesized by the in-situ reduction method, and then ... | 2016 | 26301998 |
| colorimetric aptasensor based on enzyme for the detection of vibrio parahemolyticus. | a simple colorimetric aptasensor system has been developed to detect vibrio parahemolyticus. magnetic nanoparticles (mnps) are synthesized and conjugated with specific aptamers against target and used as capture probes. in addition, this method employs gold nanoparticles (aunps) as carriers of horseradish peroxidase (hrp) and aptamers, which served as signal probes. in the presence of target, a "sandwich-type" complex of aunps-hrp-aptamer-target-aptamer-mnps is formed through specific recognitio ... | 2015 | 26302256 |
| antioxidant capacity of poly(ethylene glycol) (peg) as protection mechanism against hydrogen peroxide inactivation of peroxidases. | the ability of poly(ethylene glycol) (peg) to protect enzymatic peroxidase activity was determined for horseradish peroxidase (hrp), versatile peroxidase (vp), commercial coprinus peroxidase (bp), and chloroperoxidase (cpo). the operational stability measured as the total turnover number was determined for the four peroxidases. the presence of peg significantly increased the operational stability of vp and hrp up to 123 and 195%, respectively, and dramatically increased the total turnover number ... | 2015 | 26306530 |
| rapid legionella pneumophila determination based on a disposable core-shell fe₃o₄@poly(dopamine) magnetic nanoparticles immunoplatform. | a novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of legionella pneumophila sg1. a specific capture antibody (ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (mnps@pda-ab) and incubated with bacteria. the captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (ab-hrp), and the resulting mnps@pda-ab-legionella neumo ... | 2015 | 26320785 |
| ultrasensitive and accelerated detection of ciguatoxin by capillary electrophoresis via on-line sandwich immunoassay with rotating magnetic field and nanoparticles signal enhancement. | a sensitive and rapid on-line immunoassay for the determination of ciguatoxin ctx3c was developed based on a capillary mixing system, which was integrated with capillary electrophoresis (ce) separation and electrochemical (ec) detection. in the sandwich immunoassay system, anti-ctx3c-functionalized magnetic nanoparticles were used as immunosensing probes, and horseradish peroxidase (hrp) and anti-ctx3c antibody were bound onto the surface of gold nanoparticles (aunps) and used as recognition ele ... | 2015 | 26320955 |
| enzymatic cross-linking of α-lactalbumin to produce nanoparticles with increased foam stability. | hard colloidal nanoparticles (e.g. partly hydrophobised silica), are known to make foams with very high foam-stability. nanoparticles can also be produced from proteins by enzymatic cross-linking. such protein based particles are more suitable for food applications, but it is not known if they provide pickering foam stabilisation to the same extent as hard colloidal particles. α-lactalbumin (α-la) was cross-linked with either microbial transglutaminase (mtg) or horseradish peroxidase (hrp) to pr ... | 2015 | 26327613 |
| a recombinant antigen-based enzyme-linked immunosorbent assay (elisa) for lungworm detection in seals. | pinnipeds are frequently infected by the lungworms otostrongylus circumlitus and parafilaroides gymnurus (metastrongyloidea). infections are frequently associated with secondary bacterial bronchopneumonia and are often lethal. to date, a reliable lungworm diagnosis in individual seals is only possible during necropsy as examination of faeces collected from resting places does not allow assignment to individuals. therefore, a diagnostic tool for lungworm detection in living seals is desirable for ... | 2015 | 26329933 |
| utilization of parameters developed in layer-by-layer fabrication of protein-containing films for enzyme immobilization. | the layer-by-layer (lbl) self-assembly method has found a broad range of applications in biologically important materials. developed upon experience based on polyelectrolyte systems, various types of biomolecules have been successfully incorporated into ultrathin films with thickness in the nanometer range. we employed orthogonal experimental design to analyze the factors influencing the buildup of protein-containing lbl ultrathin films, first selecting bovine serum albumin as the exemplary prot ... | 2015 | 26330241 |
| construction of a horseradish peroxidase resistant toward hydrogen peroxide by saturation mutagenesis. | horseradish peroxidase (hrp) with a variety of potential biotechnological applications is still isolated from the horseradish root as a mixture of different isoenzymes with different biochemical properties. there is an increasing demand for preparations of high amounts of pure enzyme but its recombinant production is limited because of the lack of glycosylation in escherichia coli and different glycosylation patterns in yeasts which affects its stability parameters. the goal of this study was to ... | 2016 | 26331237 |
| sensitive electrochemical aptamer cytosensor for highly specific detection of cancer cells based on the hybrid nanoelectrocatalysts and enzyme for signal amplification. | human cancer is becoming a leading cause of death in the world and the development of a straightforward strategy for early detection of cancer is urgently required. herein, a sandwich-type electrochemical aptamer cytosensor was developed for detection of human liver hepatocellular carcinoma cells (hepg2) based on the hybrid nanoelectrocatalysts and enzyme for signal amplification. the thiolated tls11a aptamers were used as a selective bio-recognition element, attached to the gold nanoparticles ( ... | 2016 | 26332382 |
| pd-ir core-shell nanocubes: a type of highly efficient and versatile peroxidase mimic. | peroxidase mimics with dimensions on the nanoscale have received great interest as emerging artificial enzymes for biomedicine and environmental protection. while a variety of peroxidase mimics have been actively developed recently, limited progress has been made toward improving their catalytic efficiency. in this study, we report a type of highly efficient peroxidase mimic that was engineered by depositing ir atoms as ultrathin skins (a few atomic layers) on pd nanocubes (i.e., pd-ir cubes). t ... | 2015 | 26333816 |
| temperature-invariant aqueous microgels as hosts for biomacromolecules. | immobilization of enzymes on solid supports has been widely used to improve enzyme recycling, enzyme stability, and performance. we are interested in using aqueous microgels (colloidal hydrogels) as carriers for enzymes used in high-temperature reactions. these microgels should maintain their volume and colloidal stability in aqueous media up to 100 °c to serve as thermo-stable supports for enzymes. for this purpose, we prepared poly(n-hydroxyethyl acrylamide) (pheaa) microgels via a two-step sy ... | 2015 | 26335392 |
| cerebrospinal fluid aquaporin-4-immunoglobulin g disrupts blood brain barrier. | to clarify the significance of immunoglobulin g autoantibody specific for the astrocyte water channel aquaporin-4 in cerebrospinal fluid, aquaporin-4-immunoglobulin g from a neuromyelitis optica patient was administered intrathecally to naïve mice, and the distribution and pathogenic impact was evaluated. a distinct distribution pattern of aquaporin-4-immunoglobulin g deposition was observed in the subarachnoid and subpial spaces where vessels penetrate the brain parenchyma, via a paravascular r ... | 2015 | 26339679 |
| degradation-by-design: surface modification with functional substrates that enhance the enzymatic degradation of carbon nanotubes. | biodegradation of carbon-based nanomaterials has been pursued intensively in the last few years, as one of the most crucial issues for the design of safe, clinically relevant conjugates for biomedical applications. in this paper it is demonstrated that specific functional molecules can enhance the catalytic activity of horseradish peroxidase (hrp) and xanthine oxidase (xo) for the degradation of carbon nanotubes. two different azido coumarins and one cathecol derivative are linked to multi-walle ... | 2015 | 26342557 |
| ultrasensitive electrochemical immunosensor based on horseradish peroxidase (hrp)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection. | we report an ultrasensitive electrochemical immunosensor designed for the detection of protein biomarkers using horseradish peroxidase (hrp)-loaded silica-poly(acrylic acid) brushes (sio2-spaabs) as labels. hrp could be efficiently and stably accommodated in the three-dimensional architecture of the sio2-spaabs and the sio2-spaabs-hrp exhibited high catalytic performance towards o-phenylenediamine (opd) oxidation in the presence of h2o2, which resulted in significant differential pulse voltammet ... | 2016 | 26342574 |
| protein transport across membranes: comparison between lysine and guanidinium-rich carriers. | the mechanism(s) by which certain small peptides and peptide mimics carry large cargoes across membranes through exclusively non-covalent interactions has been difficult to resolve. here, we use the droplet-interface bilayer as a platform to characterize distinct mechanistic differences between two such carriers: pep-1 and a guanidinium-rich peptide mimic we call d9. while both pep-1 and d9 can carry an enzyme, horseradish peroxidase (hrp) across a lipid bilayer, we found that they do so by diff ... | 2015 | 26342679 |
| bioengineering vascularized tissue constructs using an injectable cell-laden enzymatically crosslinked collagen hydrogel derived from dermal extracellular matrix. | tissue engineering promises to restore or replace diseased or damaged tissue by creating functional and transplantable artificial tissues. the development of artificial tissues with large dimensions that exceed the diffusion limitation will require nutrients and oxygen to be delivered via perfusion instead of diffusion alone over a short time period. one approach to perfusion is to vascularize engineered tissues, creating a de novo three-dimensional (3d) microvascular network within the tissue c ... | 2015 | 26348142 |
| investigating the effect of gallium curcumin and gallium diacetylcurcumin complexes on the structure, function and oxidative stability of the peroxidase enzyme and their anticancer and antibacterial activities. | curcumin has a wide spectrum of biological and pharmacological activities including anti-inflammatory, antioxidant, antiproliferative, antimicrobial and anticancer activities. complexation of curcumin with metals has gained attention in recent years for improvement of its stability. in this study, the effect of gallium curcumin and gallium diacetylcurcumin on the structure, function and oxidative stability of horseradish peroxidase (hrp) enzyme were evaluated by spectroscopic techniques. in addi ... | 2015 | 26369539 |
| distinct enzyme-substrate interactions revealed by two dimensional kinetic comparison between dehaloperoxidase-hemoglobin and horseradish peroxidase. | the time-resolved kinetics of substrate oxidation and cosubstrate h2o2 reduction by dehaloperoxidase-hemoglobin (dhp) on a seconds-to-minutes time scale was analyzed for peroxidase substrates 2,4,6-tribromophenol (2,4,6-tbp), 2,4,6-trichlorophenol (2,4,6-tcp), and abts. substrates 2,4,6-tbp and 2,4,6-tcp show substrate inhibition at high concentration due to the internal binding at the distal pocket of dhp, whereas abts does not show substrate inhibition at any concentration. the data are consis ... | 2015 | 26375307 |
| horseradish peroxidase functionalized gold nanorods as a label for sensitive electrochemical detection of alpha-fetoprotein antigen. | in this study, a novel tracer, horseradish peroxidase (hrp) functionalized gold nanorods (au nrs) nanocomposites (hrp-au nrs), was designed to label the signal antibodies for sensitive electrochemical measurement of alpha-fetoprotein (afp). the preparation of hrp-au nrs nanocomposites and the labeling of secondary antibody (ab2) were performed by one-pot assembly of hrp and ab2 on the surface of au nrs. the immunosensor was fabricated by assembling carbon nanotubes (cnts), au nrs, and capture an ... | 2015 | 26384641 |
| a 3d µpad based on a multi-enzyme organic-inorganic hybrid nanoflower reactor. | this work reports on the development of a 3d microfluidic paper-based device (3d µpad) for glucose detection using organic-inorganic hybrid nanoflower technology to immobilize the bi-enzymatic system (glucose oxidase and horseradish peroxidase). the system is based on nanoflowerssupported on cellulose paper (the microreactor zone) coupled to 3,3',5,5'-tetramethylbenzidine (tmb) as the colorimetric probe in the detection zone. we used a digital camera for the quantitative analysis of glucose with ... | 2016 | 26386331 |
| a 3d graphene oxide microchip and a au-enwrapped silica nanocomposite-based supersandwich cytosensor toward capture and analysis of circulating tumor cells. | determination of the presence and number of circulating tumor cells (ctcs) in peripheral blood can provide clinically important data for prognosis and therapeutic response patterns. in this study, a versatile supersandwich cytosensor was successfully developed for the highly sensitive and selective analysis of ctcs using au-enwrapped silica nanocomposites (si/aunps) and three-dimensional (3d) microchips. first, 3d microchips were fabricated by a photolithography method. then, the prepared substr ... | 2015 | 26391313 |
| platinum(ii)-oligonucleotide coordination based aptasensor for simple and selective detection of platinum compounds. | wide use of platinum-based chemotherapeutic regimens for the treatment for carcinoma calls for a simple and selective detection of platinum compound in biological samples. on the basis of the platinum(ii)-base pair coordination, a novel type of aptameric platform for platinum detection has been introduced. this chemiluminescence (cl) aptasensor consists of a designed streptavidin (sa) aptamer sequence in which several base pairs were replaced by g-g mismatches. only in the presence of platinum, ... | 2015 | 26393810 |
| a cholesterol-dependent endocytic mechanism generates midbody tubules during cytokinesis. | cytokinesis is the final stage of cell division and produces two independent daughter cells. vesicles derived from internal membrane stores, such as the golgi, lysosomes, and early and recycling endosomes accumulate at the intracellular bridge (icb) during cytokinesis. here, we use electron tomography to show that many icb vesicles are not independent but connected, forming a newly described icb vesicular structure - narrow tubules that are often branched. these 'midbody tubules' labelled with h ... | 2015 | 26399547 |
| combining protein and strain engineering for the production of glyco-engineered horseradish peroxidase c1a in pichia pastoris. | horseradish peroxidase (hrp), conjugated to antibodies and lectins, is widely used in medical diagnostics. since recombinant production of the enzyme is difficult, hrp isolated from plant is used for these applications. production in the yeast pichia pastoris (p. pastoris), the most promising recombinant production platform to date, causes hyperglycosylation of hrp, which in turn complicates conjugation to antibodies and lectins. in this study we combined protein and strain engineering to obtain ... | 2015 | 26404235 |
| a novel self-assembled nano micelle as a highly efficient artificial peroxidase based on hexadecyl trimethyl ammonium bromide and cytochrome c. | a novel artificial peroxidase (ap) with highly catalytic efficiency was designed using hexadecyl trimethyl ammonium bromide (ctab, 3 mm) nano-micelles and bovine heart cytochrome c (cyt c, 0.5 µm) in 100 mm, ph 8.0 phosphate buffer at 25°c. the catalytic rate (kcat) and michaelis-menten (km) of the ap were determined to be 0.311 ± 0.013 s(-1) and 8.64 ± 0.6 µm. the catalytic efficiency was 0.0360 ± 0.0020 µm(-1)s(-1) (about 50% the efficiency of native horseradish peroxidase). the ultraviolet-vi ... | 2015 | 26406069 |
| anti-inflammatory activity of horseradish (armoracia rusticana) root extracts in lps-stimulated macrophages. | horseradish (armoracia rusticana) is a perennial crop belonging to the brassicaceae family. horseradish root is used as a condiment due to its extremely pungent flavour, deriving from the high content of glucosinolates and their breakdown products such as isothiocyanates and other sulfur compounds. horseradish also has a long history in ethnomedicine. in this study the anti-inflammatory potential of three accessions of armoracia rusticana on lipopolysaccharide from e. coli treated j774a.1 murine ... | 2015 | 26411988 |
| microgel coating of magnetic nanoparticles via bienzyme-mediated free-radical polymerization for colorimetric detection of glucose. | this study describes a new strategy for the fabrication of magnetic core-shell microgels by free-radical polymerization triggered by the cascade reaction of glucose oxidase (gox) and horseradish peroxidase (hrp). the mild polymerization around the interface of the magnetic nanoparticles permits the mild coating of the microgel layer with excellent characteristics for various applications in biocatalysis and medical diagnostics, as well as in clinical fields. the immobilized bienzyme within the m ... | 2015 | 26412343 |
| conformational changes of a chemically modified hrp: formation of a molten globule like structure at ph 5. | horseradish peroxidase is an all alpha-helical enzyme, which widely used in biochemistry applications mainly because of its ability to enhance the weak signals of target molecules. this monomeric heme-containing plant peroxidase is also used as a reagent for the organic synthesis, biotransformation, chemiluminescent assays, immunoassays, bioremediation, and treatment of wastewaters as well. accordingly, enhancing stability and catalytic activity of this protein for biotechnological uses has been ... | 2014 | 26417287 |
| hydrogen peroxide-independent generation of superoxide catalyzed by soybean peroxidase in response to ferrous ion. | it is well documented that extracellular alkalization occurs in plants under the challenges by pathogenic microbes. this may eventually induce the ph-dependent extracellular peroxidase-mediated oxidative burst at the site of microbial challenges. by employing the purified proteins of horseradish peroxidase as a model, we have recently proposed a likely role for free fe(2+) in reduction of ferric enzyme of plant peroxidases into ferrous intermediate and oxygen-bound form of enzyme known as compou ... | 2015 | 26417938 |
| target-catalyzed dna four-way junctions for cret imaging of microrna, concatenated logic operations, and self-assembly of dna nanohydrogels for targeted drug delivery. | here we report a target-catalyzed dna four-way junction (dna-4wj) on the basis of toehold-mediated dna strand displacement reaction (tm-sdr), which is readily applied in enzyme-free amplified chemiluminescence resonance energy transfer (cret) imaging of microrna. in this system, the introduction of target microrna-let-7a (mir-let-7a) activates a cascade of assembly steps with four dna hairpins, followed by a disassembly step in which the target microrna is displaced and released from dna-4wj to ... | 2015 | 26420675 |
| optimized design of a nanostructured spce-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles. | the demand for on-site nanodevices is constantly increasing. the technology development for the design of such devices is highly regarded. in this work, we report the design of a disposable platform that is structured with cauliflower-shaped gold nanoparticles (cfaunps) and we show its applications in immunosensing and enzyme-based detection. the electrochemical reduction of au(iii) allows for the electrodeposition of highly dispersed cauliflower-shaped gold nanoparticles on the surface of scree ... | 2015 | 26425435 |
| one-step protein conjugation to upconversion nanoparticles. | the emerging upconversion nanoparticles offer a fascinating library of ultrasensitive luminescent probes for a range of biotechnology applications from biomarker discovery to single molecule tracking, early disease diagnosis, deep tissue imaging, and drug delivery and therapies. the effective bioconjugation of inorganic nanoparticles to the molecule-specific proteins, free of agglomeration, nonspecific binding, or biomolecule deactivation, is crucial for molecular recognition of target molecules ... | 2015 | 26429146 |
| self-assembly of ferritin nanoparticles into an enzyme nanocomposite with tunable size for ultrasensitive immunoassay. | the self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these superstructures display collective properties that are different to those displayed by individual nanoparticles or bulk samples. however, there are increasing bottlenecks in terms of size control and multifunctionalization of nanoparticle assemblies. in this study, we developed a self-assembly strategy for construction of multifunctional nanoparticle assemblie ... | 2015 | 26431499 |
| visible-light-induced activity control of peroxidase bound to fe-doped titanate nanosheets with nanometric lateral dimensions. | catalytic performance of horseradish peroxidase (hrp) electrostatically adsorbed on nanometric and semiconducting fe-doped titanate (ft) nanosheets was successfully manipulated by visible light illumination. a colloidal solution of ft with a narrow band gap corresponding to a visible light region was fabricated through a hydrolysis reaction of metals sources. hrp could be easily bound to the ft at ph = 4 through an electrostatic interaction between them, and the formed hrp-ft was utilized for th ... | 2015 | 26439703 |
| chemiluminometric immunosensor for high-sensitivity cardiac troponin i employing a polymerized enzyme conjugate as a tracer. | to detect high-sensitivity cardiac troponin i (hs-ctni; <0.01 ng/ml) at points of care, we developed a rapid immunosensor by using horseradish peroxidase polymerized in 20 molecules on average (poly-hrp) as a tracer conjugated with streptavidin (sa-poly-hrp). as shown in the conventional system, enhanced sensitivity could be achieved by using a sequential binding scheme for the complex formation to contain the huge molecular tracer. we used a 2-dimensional chromatographic technology to carry out ... | 2015 | 26442606 |
| tetracycline impregnation affects degradation of porcine collagen matrix in healthy and diabetic rats. | the present study evaluated the degradation of collagen matrix (cm) immersed in tetracycline (ttc) or phosphate-buffered saline (pbs) in diabetic and normoglycemic rats. | 2016 | 26445855 |
| specific determination of influenza h7n2 virus based on biotinylated single-domain antibody from a phage-displayed library. | the unpredicted spread of avian influenza virus subtype h7n2 in the world is threatening animals and humans. specific and effective diagnosis and supervision are required to control the influenza. however, the existing detecting methods are laborious, are time-consuming, and require appropriate laboratory facilities. to tackle this problem, we isolated vhh antibodies against the h7n2 avian influenza virus (aiv) and performed an enzyme-linked immunosorbent assay (elisa) to detect the h7n2 virus. ... | 2016 | 26450565 |
| hierarchical porous tio2 fabricated from magnolia grandiflora petals templates for the immobilization and electrical wiring of proteins. | hierarchical porous titanium dioxide (hp-tio2) was prepared using natural magnolia grandiflora petals as templates and was further employed for the immobilization of horseradish peroxidase (hrp). scanning electron microscopy (sem) and nitrogen adsorption-desorption measurement revealed the wrinkled and porous structure of the obtained hp-tio2. fourier transform infrared spectroscopy (ft-ir) indicated the effective immobilization and conformation retention of hrp on hp-tio2. hrp immobilized on hp ... | 2015 | 26452785 |
| enzyme catalytic amplification of mirna-155 detection with graphene quantum dot-based electrochemical biosensor. | a specific and sensitive method was developed for quantitative detection of mirna by integrating horseradish peroxidase (hrp)-assisted catalytic reaction with a simple electrochemical rna biosensor. the electrochemical biosensor was constructed by a double-stranded dna structure. the structure was formed by the hybridization of thiol-tethered oligodeoxynucleotide probes (capture dna), assembled on the gold electrode surface, with target dna and aminated indicator probe (nh2-dna). after the const ... | 2016 | 26453906 |
| pathogenesis of optic disc edema in raised intracranial pressure. | optic disc edema in raised intracranial pressure was first described in 1853. ever since, there has been a plethora of controversial hypotheses to explain its pathogenesis. i have explored the subject comprehensively by doing basic, experimental and clinical studies. my objective was to investigate the fundamentals of the subject, to test the validity of the previous theories, and finally, based on all these studies, to find a logical explanation for the pathogenesis. my studies included the fol ... | 2016 | 26453995 |
| aptamer-phage reporters for ultrasensitive lateral flow assays. | we introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. m13 phage displaying an in vivo biotinylatable peptide (avitag) genetically fused to the phage tail protein piii were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (hrp) reporter enzymes covalently attached to the pviii coat prot ... | 2015 | 26456715 |
| biocatalytic etching of semiconductor cadmium sulfide nanoparticles as a new platform for the optical detection of analytes. | we report for the first time the enzymatic etching of cadmium sulfide nanoparticles (cds nps). the fluorescence of semiconductor cds nps is modulated irreversibly by an enzymatic reaction catalyzed by horseradish peroxidase (hrp). we observed blue-shifts of the corresponding fluorescence peaks for cds nps and a decrease in the intensity of the fluorescence signal. | 2015 | 26456861 |
| preparation and preliminary application of monoclonal antibodies to the receptor binding region of clostridium difficile toxin b. | a previous nationwide chinese epidemiological study revealed through isolation of a‑b+ clostridium difficile strains, which produce toxin b (tcdb), but not toxin a tcda, that the strains are widespread and more frequent in east asian countries,. the development of a process capable of detecting tcdb is required in microbiological laboratories in order to facilitate the control of the a‑b+ c. difficile strains, however, no diagnostic reagents have been developed to date. the aim of the present st ... | 2015 | 26459027 |
| horseradish peroxidase-mediated, iodide-catalyzed cascade reaction for plasmonic immunoassays. | this report outlines an enzymatic cascade reaction for signal transduction and amplification for plasmonic immunoassays by using horseradish peroxidase (hrp)-mediated aggregation of gold nanoparticles (aunps). hrp-catalyzed oxidation of iodide and iodide-catalyzed oxidation of cysteine is employed to modulate the plasmonic signals of aunps. it agrees well with the current immunoassay platforms and allows naked-eye readout with enhanced sensitivity, which holds great promise for applications in r ... | 2015 | 26460152 |
| the free heme concentration in healthy human erythrocytes. | heme, the prosthetic group of hemoglobin, may be released from its host due to an intrinsic instability of hemoglobin and accumulate in the erythrocytes. free heme is in the form of hematin (fe(3+) protoporphyrin ix oh) and follows several pathways of biochemical toxicity to tissues, cells, and organelles since it catalyzes the production of reactive oxygen species. to determine concentration of soluble free heme in human erythrocytes, we develop a new method. we lyse the red blood cells and iso ... | 2015 | 26460266 |
| singlet molecular oxygen generated in dark biological process. | ultraweak chemiluminescence arising from biomolecules oxidation has been attributed to the radiative deactivation of singlet molecular oxygen [(1)o2] and electronically excited triplet carbonyl products involving dioxetane intermediates. as examples, we will discuss the generation of (1)o2 from lipid hydroperoxides, which involves a cyclic mechanism from a linear tetraoxide intermediate. the generation of (1)o2 in aqueous solution via energy transfer from the excited triplet acetone arising from ... | 2014 | 26461325 |
| inflammation-enhanced drug-induced liver injury. | drug-induced liver injury is a major concern in clinical studies as well as in post-marketing surveillance. previous evidence suggests that drug exposure during periods of inflammation can increase an individual's susceptibility to toxicity. inflammation caused by infections or endotoxin markedly activates nadph oxidase that generates superoxide radicals by transferring electrons from nadph. in the phagosome, superoxide radicals spontaneously form hydrogen peroxide (h2o2) and other reactive oxyg ... | 2014 | 26461367 |
| single nanoparticle to 3d supercage: framing for an artificial enzyme system. | a facile strategy has been developed to fabricate cu(oh)2 supercages (scs) as an artificial enzyme system with intrinsic peroxidase-mimic activities (pma). scs with high catalytic activity and excellent recyclability were generated via direct conversion of amorphous cu(oh)2 nanoparticles (nps) at room temperature. more specifically, the process that takes a single nanoparticle to a 3d supercage involves two basic steps. first, with addition of a copper-ammonia complex, the cu(2+) ions that are l ... | 2015 | 26464081 |
| single chip spr and fluorescent elisa assay of prostate specific antigen. | a multi-channel system combining fluidics and micropatterned plasmonic materials with wavelength interrogation surface plasmon resonance (spr) and fluorescence detection was integrated from the combination of a small and motorized fluorescence microscope mounted on a portable 4-channel spr instrument. the spr and fluorescent measurements were performed based on the same detection area in a multi-channel fluidic, with a sensing scheme for prostate-specific antigen (psa) consisting of a sandwich a ... | 2015 | 26467689 |
| detection of ochratoxin a (ota) in coffee using chemiluminescence resonance energy transfer (cret) aptasensor. | we report a chemiluminescence resonance energy transfer (cret) aptasensor for the detection of ochratoxin a (ota) in roasted coffee beans. the aptamer sequences used in this study are 5'-dnazyme-linker-ota aptamer-3'-dabcyl. dabcyl at the end of the ota aptamer region plays as a quencher in cret aptasensor. when hemin and ota are added, the dabcyl-labeled ota aptamer approaches to the g-quadruplex-hemin complex by formation of the g-quadruplex-ota complex. the g-quadruplex-hemin complexes posses ... | 2016 | 26471659 |
| development of a novel method for determination of mercury based on its inhibitory effect on horseradish peroxidase activity followed by monitoring the surface plasmon resonance peak of gold nanoparticles. | a highly sensitive and simple indirect spectrophotometric method has been developed for the determination of trace amounts of inorganic mercury (hg(2+)) in aqueous media. the method is based on the inhibitory effect of hg(2+) on the activity of horseradish peroxidase (hrp) in the oxidation of ascorbic acid by hydrogen peroxide followed by the reduction of au(3+) to au-nps by unreacted ascorbic acid and the measurement of the absorbance of localized surface plasmon resonance (lspr) peak of gold n ... | 2016 | 26474243 |
| accurate detection of adenylation domain functions in nonribosomal peptide synthetases by an enzyme-linked immunosorbent assay system using active site-directed probes for adenylation domains. | a significant gap exists between protein engineering and enzymes used for the biosynthesis of natural products, largely because there is a paucity of strategies that rapidly detect active-site phenotypes of the enzymes with desired activities. herein, we describe a proof-of-concept study of an enzyme-linked immunosorbent assay (elisa) system for the adenylation (a) domains in nonribosomal peptide synthetases (nrpss) using a combination of active site-directed probes coupled to a 5'-o-n-(aminoacy ... | 2015 | 26474351 |
| electrochemical sensing platforms based on the different carbon derivative incorporated interface. | their effects on the properties of these biosensors. biosensors were prepared by horseradish peroxidase (hrp) immobilization on the composite electrodes composed of carbon black, carbon nanofiber (cnf), extended graphite, multiwalled carbon nanotube (mwcnt), reduced graphene oxide (rego) and poly(glycidyl methacrylateco-vinylferrocene) (p(gma-co-vfc)) as mediator, covalent linker, and host matrix for carbon derivatives. the modified pencil graphite electrode (pge) was used for the detection of h ... | 2016 | 26478373 |
| thionine-modified poly(glycidyl methacrylate) nanospheres as labels of antibodies for biosensing applications. | monodisperse poly(glycidyl methacrylate) (pgma) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. the effects of various reaction parameters on the particle properties were investigated. the particle size was controlled in the range of 350-420 nm. to introduce carboxyl groups, the pgma nanospheres were hydrolyzed and oxidized with kmno4. subsequently, the enzyme horseradish peroxidase (hrp) and the electron mediator thionine were c ... | 2015 | 26479119 |
| tailor-made boronic acid functionalized magnetic nanoparticles with a tunable polymer shell-assisted for the selective enrichment of glycoproteins/glycopeptides. | biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. in this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multifunctionalized magnetic nanoparticles (nps) using a distillation-precipitation polymerization (dpp) technique. the polymer shell is obtained through copolymerization of two monomers-affinity ligand 3-acrylaminophenylboronic acid (aapba) and a hydrophilic functional monomer. the ... | 2015 | 26479332 |
| click synthesis of hydrophilic maltose-functionalized iron oxide magnetic nanoparticles based on dopamine anchors for highly selective enrichment of glycopeptides. | the development of methods to isolate and enrich low-abundance glycopeptides from biological samples is crucial to glycoproteomics. herein, we present an easy and one-step surface modification strategy to prepare hydrophilic maltose functionalized fe3o4 nanoparticles (nps). first, based on the chelation of the catechol ligand with iron atoms, azido-terminated dopamine (da) derivative was assembled on the surface of magnetic fe3o4 nanoparticles by sonication. second, the hydrophilic maltose-funct ... | 2015 | 26479949 |
| bio-based wrinkled surfaces harnessed from biological design principles of wood and peroxidase activity. | a new and simple approach for surface wrinkling inspired by polymer assemblies in wood fibers is introduced. a hard skin is synthesized on a linear polysaccharide support that resembles the structural units of the cell wall. this skin, a wood mimetic layer, is produced through immersion in a solution containing phenolic precursor and subsequent surface reaction by horseradish peroxidase. a patterned surface with micron-scale wrinkles is formed upon drying and as a result of inhomogeneous shrinka ... | 2015 | 26489384 |
| general approach to the immobilization of glycoenzyme chains inside calcium alginate beads for bioassay. | a general method to obtain the efficient entrapment of mixtures of glycoenzymes in calcium alginate hydrogel is proposed in this paper. as a proof of principle, three glycoenzymes acting in series (trehalase, glucose oxidase, and horseradish peroxidase) have been coimmobilized in calcium alginate beads. the release of the enzymes from the hydrogel mesh (leakage) is avoided by exploiting the enzyme's aggregation induced by the concanavalin a. the aggregation process has been monitored by dynamic ... | 2015 | 26489723 |
| gelatin-loaded p(hema-gma) cryogel for high-capacity immobilization of horseradish peroxidase. | poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(hema-gma)] cryogel discs were prepared under sub-zero temperatures. gelatin was attached covalently on the p(hema-gma) cryogel discs and reversible immobilization of horseradish peroxidase (hrp) was performed. the p(hema-gma) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. hrp immobilization capacity of p(hema-gma)/gelatin cryogel discs was 24.8 mg/g. removal of phenol from ... | 2016 | 26508304 |
| interrogating the activities of conformational deformed enzyme by single-molecule fluorescence-magnetic tweezers microscopy. | characterizing the impact of fluctuating enzyme conformation on enzymatic activity is critical in understanding the structure-function relationship and enzymatic reaction dynamics. different from studying enzyme conformations under a denaturing condition, it is highly informative to manipulate the conformation of an enzyme under an enzymatic reaction condition while monitoring the real-time enzymatic activity changes simultaneously. by perturbing conformation of horseradish peroxidase (hrp) mole ... | 2015 | 26512103 |
| intrauterine growth restriction in neonatal piglets affects small intestinal mucosal permeability and mrna expression of redox-sensitive genes. | neonates with intrauterine growth restriction (iugr) show lower efficiency of nutrient utilization compared to normal birth weight (nbw) newborns. this study was conducted using neonatal piglets as a model to test the hypothesis that iugr affects the intestinal barrier function, intestinal structure, and antioxidant system development during the suckling period. the small intestinal mucosae were obtained from iugr and nbw littermates in the suckling period (d 0, 3, 8, and 19 postnatal). the epit ... | 2016 | 26514167 |
| the biological effects of vanadyl curcumin and vanadyl diacetylcurcumin complexes: the effect on structure, function and oxidative stability of the peroxidase enzyme, antibacterial activity and cytotoxic effect. | curcumin has multiple pharmacological effects, but it has poor stability. complexation of curcumin with metals improves its stability. here, the effects of vanadyl curcumin and vanadyl diacetylcurcumin on the function and structure of horseradish peroxidase enzyme were evaluated by spectroscopic techniques. cytotoxic effect of the complexes was also assessed on mcf-7 breast cancer, bladder and lncap prostate carcinoma cell line. the results showed that the complexes improve catalytic activity of ... | 2016 | 26516945 |
| transformation, products, and pathways of chlorophenols via electro-enzymatic catalysis: how to control toxic intermediate products. | chlorophenols can be easily oxidized into chlorobenzoquinones (cbqs), which are highly toxic and have been linked to bladder cancer risk. herein, we report the transformation, products, and pathways of 2,4-dichlorophenol (dcp) by horseradish peroxidase (hrp) and electro-generated hydrogen peroxide (h2o2) and suggest methods to control the formation of toxic intermediate products. after a 10-min electroenzymatic process, 99.7% dcp removal may be achieved under optimal conditions. a total of 16 re ... | 2016 | 26519798 |
| perineural capsaicin induces the uptake and transganglionic transport of choleratoxin b subunit by nociceptive c-fiber primary afferent neurons. | the distribution of spinal primary afferent terminals labeled transganglionically with the choleratoxin b subunit (ctb) or its conjugates changes profoundly after perineural treatment with capsaicin. injection of ctb conjugated with horseradish peroxidase (hrp) into an intact nerve labels somatotopically related areas in the ipsilateral dorsal horn with the exceptions of the marginal zone and the substantia gelatinosa, whereas injection of this tracer into a capsaicin-pretreated nerve also resul ... | 2015 | 26520849 |
| quantification of sorafenib in human serum by competitive enzyme-linked immunosorbent assay. | the multikinase inhibitor sorafenib has been used in the treatment of hepatocellular carcinoma, renal cell carcinoma, and differentiated thyroid carcinoma. here we have demonstrated the production of the first specific antibody against sorafenib. anti-sorafenib serum was obtained by immunizing mice with an antigen conjugated with bovine serum albumin and carboxylic modified 4-(4-aminophenoxy)-n-methyl-2-pyridinecarboxamide (ampc) using the n-succinimidyl ester method. enzyme labeling of sorafeni ... | 2015 | 26521829 |
| [goldmag particle-based chemiluminescence immunoassay for human high sensitive c-reactive protein]. | to develop a sensitive, accurate detection method for high sensitive c-reactive protein (hscrp). | 2015 | 26522353 |
| nanoprobe-initiated enzymatic polymerization for highly sensitive electrochemical dna detection. | electrochemical dna (e-dna) sensors have been greatly developed and play an important role in early diagnosis of different diseases. to determine the extremely low abundance of dna biomarkers in clinical samples, scientists are making unremitting efforts toward achieving highly sensitive and selective e-dna sensors. here, a novel e-dna sensor was developed taking advantage of the signal amplification efficiency of nanoprobe-initiated enzymatic polymerization (niep). in the niep based e-dna senso ... | 2015 | 26524941 |
| shenqi fuzheng injection attenuates irradiation-induced brain injury in mice via inhibition of the nf-κb signaling pathway and microglial activation. | radiation-induced brain injury (ribi) is the most common and severe adverse effect induced by cranial radiation therapy (crt). in the present study, we examined the effects of the traditional chinese medicine shenqi fuzheng injection (sfi) on ribi in mice, and explored the underlying mechanisms. | 2015 | 26526200 |
| detection of hydrogen peroxide in photosystem ii (psii) using catalytic amperometric biosensor. | hydrogen peroxide (h2o2) is known to be generated in photosystem ii (psii) via enzymatic and non-enzymatic pathways. detection of h2o2 by different spectroscopic techniques has been explored, however its sensitive detection has always been a challenge in photosynthetic research. during the recent past, fluorescence probes such as amplex red (ar) has been used but is known to either lack specificity or limitation with respect to the minimum detection limit of h2o2. we have employed an electrochem ... | 2015 | 26528319 |
| sensitive electrochemical immunoassay for chlorpyrifos by using flake-like fe3o4 modified carbon nanotubes as the enhanced multienzyme label. | a highly sensitive electrochemical immunoassay of chlorpyrifos (cpf) was developed by using a biocompatible quinone-rich polydopamine nanospheres modified glass carbon electrode as the sensor platform and multi-horseradish peroxidase-flake like fe3o4 coated carbon nanotube nanocomposites as the signal label. due to the quinone-rich polydopamine nanospheres, the platform exhibited excellent fixing capacity by simple coating of sticky polydopamine nanospheres and subsequent oxidization. by copreci ... | 2015 | 26547497 |
| horseradish peroxidase and aptamer dual-functionalized nanoprobe for the amplification detection of alpha-methylacyl-coa racemase. | alpha-methylacyl-coa racemase (amacr) is over-expressed in many cancer types and can serve as a novel diagnostic biomarker. development of convenient and sensitive detection methods of amacr is of particular importance for cancer diagnosis. aptamers are a type of recognition elements, which possess many advantages over antibody, making them suitable for applications in biosensing and biotechnology. in this work, we use the efficient surface modification of gold nanoparticles (aunps) to prepare t ... | 2015 | 26547498 |
| functionalized nanofiber meshes enhance immunosorbent assays. | three-dimensional substrates with high surface-to-volume ratios and subsequently large protein binding capacities are of interest for advanced immunosorbent assays utilizing integrated microfluidics and nanosensing elements. a library of bioactive and antifouling electrospun nanofiber substrates, which are composed of high-molecular-weight poly(oxanorbornene) derivatives, is described. specifically, a set of copolymers are synthesized from three 7-oxanorbornene monomers to create a set of water ... | 2015 | 26551162 |
| which one of the two common reporter systems is more suitable for chemiluminescent enzyme immunoassay: alkaline phosphatase or horseradish peroxidase? | alkaline phosphatase and horseradish peroxidase are the most commonly used reporter systems in chemiluminescent enzyme immunoassay (cleia). which one, therefore, would be better when establishing a cleia method for a new target substance? there was no standard answer. in this study, both reporters were compared systematically including luminescence kinetics, conjugation methods, optimal condition and detection performance, using two common drugs, sd-methoxy-pyrimidine and enrofloxacin, as determ ... | 2016 | 26552992 |
| pioneer neurons of the antennal nervous system project to protocerebral pioneers in the grasshopper schistocerca gregaria. | the twin nerve tracts of the antenna of the grasshopper schistocerca gregaria are established early in embryogenesis by sibling pairs of pioneers which delaminate from the epithelium into the lumen at the antennal tip. these cells can be uniquely identified via their co-expression of the neuronal labels horseradish peroxidase and the lipocalin lazarillo. the apical pioneers direct axons toward the antennal base where they encounter guidepost-like cells called base pioneers which transiently expr ... | 2015 | 26553379 |
| synthesis and characterization of an in situ forming hydrogel using tyramine conjugated high methoxyl gum tragacanth. | in this study, an enzyme catalyzed in situ forming hydrogel based on tyramine conjugated high methoxyl content gum tragacanth (ta-hmgt) was prepared and characterized. ta-hmgt was synthesized via heterogeneous ammonolysis of methyl ester groups of hmgt. then, the hydrogel was prepared via horseradish peroxidase catalyzed coupling reaction in the presence of hydrogen peroxide. hydrogel properties, such as gelation time, swelling/degradation behavior and rheological properties could be adjusted by ... | 2016 | 26553882 |
| synthesis and characterization of starch-poly(methyl acrylate) graft copolymers using horseradish peroxidase. | horseradish peroxidase (hrp)-mediated graft polymerization in the presence of hydrogen peroxide (h2o2) and acetylacetone (acac) has been successfully applied to the synthesis of starch-poly(methyl acrylate) (pma). the graft copolymer was characterized by fourier transform infrared (ft-ir), elemental analysis, nuclear magnetic resonance ((1)h nmr and (13)c nmr), and differential scanning calorimetry (dsc). ft-ir, elemental analysis and nmr confirmed that methyl acrylate (ma) was grafted onto star ... | 2016 | 26572441 |
| modeling of enhanced catalysis in multienzyme nanostructures: effect of molecular scaffolds, spatial organization, and concentration. | colocalized multistep enzymatic reaction pathways within biological catabolic and metabolic processes occur with high yield and specificity. spatial organization on membranes or surfaces may be associated with increased efficiency of intermediate substrate transfer. using a new brownian dynamics package, geombd, we explored the geometric features of a surface-anchored enzyme system by parallel coarse-grained brownian dynamics simulations of substrate diffusion over microsecond (μs) to millisecon ... | 2015 | 26574226 |
| carboxylesterase converts amplex red to resorufin: implications for mitochondrial h2o2 release assays. | amplex red is a fluorescent probe that is widely used to detect hydrogen peroxide (h2o2) in a reaction where it is oxidised to resorufin by horseradish peroxidase (hrp) as a catalyst. this assay is highly rated amongst other similar probes thanks to its superior sensitivity and stability. however, we report here that amplex red is readily converted to resorufin by a carboxylesterase without requiring h2o2, horseradish peroxidase or oxygen: this reaction is seen in various tissue samples such as ... | 2016 | 26577176 |
| development of a c3c-based elisa method for the determination of anti-complementary potency of bupleurum polysaccharides. | traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. however, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. the main objective of our study was to develop a specific enzyme-linked immunosorbent assay (elisa) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. by using ... | 2015 | 26579461 |
| enhancement of the chemiluminescence response of enzymatic reactions by plasmonic surfaces for biosensing applications. | we report the enhancement of chemiluminescence response of horseradish peroxidase (hrp) in bioassays by plasmonic surfaces, which are comprised of (i) silver island films (sifs) and (ii) metal thin films (silver, gold, copper, and nickel, 1 nm thick) deposited onto glass slides. a model bioassay, based on the interactions of avidin-modified hrp with a monolayer of biotinylated poly(ethylene-glycol)-amine, was employed to evaluate the ability of plasmonic surfaces to enhance chemiluminescence res ... | 2017 | 26582101 |
| phosphopeptide detection with biotin-labeled phos-tag. | protein kinases are widely considered to be invaluable target enzymes for drug discovery and for diagnosing diseases and assessing their prognosis. effective analytical techniques for measuring the activities of cellular protein kinases are therefore required for studies in the field of phosphoproteomics. we have recently developed a highly sensitive microarray-based technique for tracing the activities of protein kinases. a series of peptides that are specific substrates of various protein kina ... | 2016 | 26584916 |
| polydimethylsiloxane microfluidic chemiluminescence immunodevice with the signal amplification strategy for sensitive detection of human immunoglobin g. | a polydimethylsiloxane (pdms) microfluidic chemiluminescence (cl) immunodevice for sensitive detection of human immunoglobin g (igg) with the signal amplification strategy was developed in this work. the immunodevice was prepared by covalently immobilizing capture antibodies (abs) on the silanized microchannel of microfluidic chip. gold nanoparticles (aunps) functionalized with a high molar ratio of horseradish peroxidase (hrp) were used as an ab label for signal amplification. using a sandwich ... | 2016 | 26592629 |
| feedback from visual cortical area 7 to areas 17 and 18 in cats: how neural web is woven during feedback. | to investigate the feedback effect from area 7 to areas 17 and 18, intrinsic signal optical imaging combined with pharmacological, morphological methods and functional magnetic resonance imaging (fmri) was employed. a spatial frequency-dependent decrease in response amplitude of orientation maps was observed in areas 17 and 18 when area 7 was inactivated by a local injection of gaba, or by a lesion induced by liquid nitrogen freezing. the pattern of orientation maps of areas 17 and 18 after the ... | 2016 | 26592718 |
| self-propelled enzyme-based motors for smart mobile electrochemical and optical biosensing. | a millimeter-sized tubular motor for mobile biosensing of h2o2 in environmental and relevant clinical samples is reported. the concept relies on the self-propelled motion by the marangoni effect, where the asymmetric release of sds surfactant induces fluid convection and rapid dispersion of horseradish peroxidase (hrp) enzyme into the sample solution. this efficient movement together with the continuous release of fresh enzyme leads to greatly accelerated enzymatic reaction processes without the ... | 2015 | 26595193 |
| transplantation of erythropoietin gene-modified neural stem cells improves the repair of injured spinal cord. | the protective effects of erythropoietin on spinal cord injury have not been well described. here, the eukaryotic expression plasmid pcdna3.1 human erythropoietin was transfected into rat neural stem cells cultured in vitro. a rat model of spinal cord injury was established using a free falling object. in the human erythropoietin-neural stem cells group, transfected neural stem cells were injected into the rat subarachnoid cavity, while the neural stem cells group was injected with non-transfect ... | 2015 | 26604911 |
| a reusable electrochemical immunosensor fabricated using a temperature-responsive polymer for cancer biomarker proteins. | in the present study, we describe a reusable electrochemical immunosensor for the repeated detection of cancer biomarkers using a single platform. the integration of a temperature-responsive polymer on the electrode surface enables easy manipulation of the biological sensing interface (i.e., addition of biotin, streptavidin, and antibody), thus allowing for temperature-induced regeneration and disruption of the interface architecture of the electrode surface. using our immunosensor, we demonstra ... | 2016 | 26606310 |
| mechanism of the biomolecular synthesis of pedot:pss: importance of heme degradation by hydrogen peroxide. | the use of biomolecules as oxidants for the synthesis of conducting polymers provides an important tool for the control of polymer properties. using | 2016 | 26609551 |
| the fucomic potential of mosquitoes: fucosylated n-glycan epitopes and their cognate fucosyltransferases. | fucoconjugates are key mediators of protein-glycan interactions in prokaryotes and eukaryotes. as examples, n-glycans modified with the non-mammalian core α1,3-linked fucose have been detected in various organisms ranging from plants to insects and are immunogenic in mammals. the rabbit polyclonal antibody raised against plant horseradish peroxidase (anti-hrp) is able to recognize the α1,3-linked fucose epitope and is also known to specifically stain neural tissues in the fruit fly drosophila me ... | 2015 | 26617287 |
| improvements in neuroelectrophysiological and rear limb functions in rats with spinal cord injury after schwann cell transplantation in combination with a c5a receptor antagonist. | we measured the effect of schwann cell transplantation and complement factor 5a (c5a) receptor antagonist on nerve function recovery in rats with spinal cord injury. experimental spinal cord injury was induced in eighty wistar rats and these were randomly divided into four treatment groups: culture medium and saline injection (control group), schwann cell injection (cell transplantation group), c5a receptor antagonist injection (c5a receptor antagonist group), and both schwann cell and c5a recep ... | 2015 | 26634479 |
| plasmonic enzyme-linked immunosorbent assay using nanospherical brushes as a catalase container for colorimetric detection of ultralow concentrations of listeria monocytogenes. | plasmonic enzyme-linked immunosorbent assay (pelisa) based on catalase (cat)-mediated gold nanoparticle growth exhibits ultrahigh sensitivity for detecting disease-related biomarkers using sandwich formats. however, the limit of detection (lod) of this strategy for listeria monocytogenes is only around 10(3) cfu/ml, which considerably exceeds the amount of l. monocytogenes commonly present in food products (<100 cfu/g). herein, we report an improved pelisa method for detection of l. monocytogene ... | 2015 | 26646325 |
| electrochemical immunosensor for interferon-γ based on disposable ito detector and hrp-antibody-conjugated nano gold as signal tag. | tuberculosis is the most frequent cause of infection-related death worldwide. a new disposable electrochemical immunosensor with low cost and simple fabrication was proposed to detect interferon-γ (ifn-γ). diallyldimethylammonium chloride (pdda) and au nanoparticle (aunp) composite were used to provide an efficient biointerface, horseradish peroxidase (hrp)-labeled antibody-conjugated aunp (hrp-ab2-aunp) bioconjugates were used as a novel signal tag. the large amounts of hrp on the signal tag ca ... | 2016 | 26652410 |
| employment of bromophenol red and bovine serum albumin as luminol signal co-enhancer in chemiluminescent detection of sequence-specific dna. | bromophenol red, known as chemical indicator, was found to act as a novel potent signal enhancer of the peroxidase-catalyzed luminol-h2o2 chemiluminescent (cl) reaction. it was found interestingly that bovine serum albumin (bsa) played a role in the enhanced chemiluminescent reaction (ecr). the addition of 2.5 mg ml(-1) bsa into bromophenol red-enhance cl system showed 36 times stronger cl signal than that without addition of bsa. mechanism study showed that the luminophors in the ecr were still ... | 2016 | 26653448 |
| a sensitive surface-enhanced raman scattering enzyme-catalyzed immunoassay of respiratory syncytial virus. | respiratory viruses have become a major global health challenge which would benefit from advances in screening methods for early diagnosis. respiratory syncytial virus (rsv) is one of the most important pathogen causing severe lower respiratory tract infections. here we present a novel surface-enhanced raman scattering (sers) enzyme-catalyzed immunoassay of rsv by employing peroxidase substrate 3, 3'-5, 5'-tetramethylbenzidine (tmb) as raman molecule. horseradish peroxidase (hrp) attached to the ... | 2016 | 26653454 |
| effects of beta-hydroxybutyrate on brain vascular permeability in rats with traumatic brain injury. | this study investigates the effect of beta-hydroxybutyrate (bhb) on blood-brain barrier (bbb) integrity during traumatic brain injury (tbi) in rats. evans blue (eb) and horseradish peroxidase (hrp) were used as determinants of bbb permeability. glutathione (gsh) and malondialdehyde (mda) levels were estimated in the right (injury side) cerebral cortex of animals. the gene expression levels for occludin, glucose transporter (glut)-1, aquaporin4 (aqp4) and nuclear factor-kappab (nf-κb) were perfor ... | 2016 | 26656066 |
| magnetic nanoparticles pcr enzyme-linked gene assay for quantitative detection of bcr/abl fusion gene in chronic myelogenous leukemia. | magnetic nanoparticles (mnps) have been widely used in medical diagnostic research. in this work, two technologies, mnps and polymerase chain reaction (pcr), were combined to increase detection sensitivity and specificity. a novel technique based on the mnps-pcr enzyme-linked gene assay (melga) was developed for detection of the bcr/abl abnormal gene in chronic myelogenous leukemia (cml) patients. | 2016 | 26667895 |
| ultrasensitive analysis of glucose in serum by capillary electrophoresis with lif detection in combination with signal amplification strategies and on-column enzymatic assay. | a highly specific and sensitive method for glucose quantification in human serum samples based on on-column enzymatic assay is described. in this method, the head of the capillary was used as a nanoliter-microreactor, the diluted samples spiked with a novel fluorogenic reagent named 2-[6-(4'-amino) phenoxy-3h-xanthen-3-on-9-yl] benzoic acid (apf), and the mixed enzyme solutions of glucose oxidase (gox) and horseradish peroxidase (hrp), were individually injected into the capillary. hydrogen pero ... | 2016 | 26668076 |