Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins. | screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. the standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. a sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin g and captured ovalbumin molecules, and secondary anti-o ... | 2015 | 25963896 |
| a polyaniline based ultrasensitive potentiometric immunosensor for cardiac troponin complex detection. | an ultrasensitive immunosensor based on potentiometric elisa for the detection of a cardiac biomarker, troponin i-t-c (tn i-t-c) complex, was developed. the sensor fabrication involves typical sandwich elisa procedures, while the final signal readout was achieved using open circuit potentiometry (ocp). glassy carbon (gc) working electrodes were first coated with emulsion-polymerized polyaniline/dinonylnaphthalenesulfonic acid (pani/dnnsa) and the coated surface was utilized as a transducer layer ... | 2015 | 25966464 |
| highly sensitive and robust peroxidase-like activity of porous nanorods of ceria and their application for breast cancer detection. | porous nanorods of ceria (pn-ceria), a novel ceria nanostructure with a large surface area and a high surface ce(3+) fraction, exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate in the presence of h2o2. peroxidase-like activity of ceria originated from surface ce(3+) species as the catalytic center, thereby explaining the high performance of pn-ceria as an artificial enzyme mimicking peroxidase. compared with the natural enzyme horseradish peroxidase (hrp), pn ... | 2015 | 25968461 |
| determination of minimum enzymatic decolorization time of reactive dye solution by spectroscopic & mathematical approach. | synthetic dyes are very important for textile dyeing, paper printing, color photography and petroleum products. traditional methods of dye removal include biodegradation, precipitation, adsorption, chemical degradation, photo degradation, and chemical coagulation. dye decolorization with enzymatic reaction is an important issue for several research field (chemistry, environment) in this study, minimum decolorization time of remazol brilliant blue r dye with horseradish peroxidase enzyme was calc ... | 2015 | 25970889 |
| a general method for synthesizing enzyme-polymer conjugates in reverse emulsions using pluronic as a reactive surfactant. | using aldehyde-functionalized pluronic as the reactive surfactant, enzyme-pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. the versatility of this method was validated using horseradish peroxidase (hrp), candida rugosa lipase (crl) and candida antarctica lipase b (calb). the resulting ... | 2015 | 25977947 |
| a combination of electro-enzymatic catalysis and electrocoagulation for the removal of endocrine disrupting chemicals from water. | we in this study investigated a novel electrochemical approach combining electro-enzyme and electrocoagulation to precipitate bisphenol a (bpa) from water containing humic acid (ha). horseradish peroxidase was immobilized on the graphite felt of ti electrode as hrp-gf/ti cathode, with aluminum plate anode establishing a pair of working electrodes. bpa was 100% removed and the reduction of total organic carbon (toc) reached 95.1% after 20-min sequencing treatment with the current density of 2.3 m ... | 2015 | 25978190 |
| high-yield reactivation of anionic tobacco peroxidase overexpressed in escherichia coli. | anionic tobacco peroxidase (top) is extremely active in chemiluminescence reaction of luminol oxidation without addition of enhancers and more stable than horseradish peroxidase under antibody conjugation conditions. in addition, recombinant top (rtop) produced in escherichia coli is known to be a perfect direct electron transfer catalyst on electrodes of various origin. these features make the task of development of a high-yield reactivation protocol for rtop practically important. previous att ... | 2015 | 25986322 |
| detection of trace heavy metal ions in water by nanostructured porous si biosensors. | a generic biosensing platform, based on nanostructured porous si (psi), fabry-pérot thin films, for label-free monitoring of heavy metal ions in aqueous solutions by enzymatic activity inhibition, is described. first, we show a general detection assay by immobilizing horseradish peroxidase (hrp) within the oxidized psi nanostructure and monitor its catalytic activity in real time by reflective interferometric fourier transform spectroscopy. optical studies reveal the high specificity and sensiti ... | 2015 | 25988196 |
| ultra-high-throughput screening of an in vitro-synthesized horseradish peroxidase displayed on microbeads using cell sorter. | the c1a isoenzyme of horseradish peroxidase (hrp) is an industrially important heme-containing enzyme that utilizes hydrogen peroxide to oxidize a wide variety of inorganic and organic compounds for practical applications, including synthesis of fine chemicals, medical diagnostics, and bioremediation. to develop a ultra-high-throughput screening system for hrp, we successfully produced active hrp in an escherichia coli cell-free protein synthesis system, by adding disulfide bond isomerase dsbc a ... | 2015 | 25993095 |
| an electrochemical micrornas biosensor with the signal amplification of alkaline phosphatase and electrochemical-chemical-chemical redox cycling. | micrornas (mirnas) have been regarded as clinically important biomarkers and drug discovery targets. in this work, we reported a simple and ultrasensitive electrochemical method for mirnas detection based on single enzyme amplification and electrochemical-chemical-chemical (ecc) redox cycling. specifically, upon contact with the target mirnas, the hairpin structure of biotinylated dna immobilized on gold electrode was destroyed and the biotin group in dna was forced away from the electrode surfa ... | 2015 | 26002330 |
| enzyme catalytic nitration of aromatic compounds. | nitroaromatic compounds are important intermediates in organic synthesis. the classic method used to synthesize them is chemical nitration, which involves the use of nitric acid diluted in water or acetic acid, both harmful to the environment. with the development of green chemistry, environmental friendly enzyme catalysis is increasingly employed in chemical processes. in this work, we adopted a non-aqueous horseradish peroxidase (hrp)/nano2/h2o2 reaction system to study the structural characte ... | 2015 | 26002502 |
| high throughput flow injection bioluminometric method for olive oil antioxidant capacity. | this paper describes a rapid flow injection automated method for the determination of olive oil total antioxidant capacity. the chemistry involved is the horseradish peroxidase (hrp) catalysed oxidation of luminol by hydrogen peroxide. oxidation results in light emission (bioluminescence) that is enhanced using p-iodophenol sensitizer. olive oil (0.7ml) is extracted with two 0.7ml aliquots of 80-20% (v/v) methanol-water solvent. a 17μl aliquot of the extract containing hydrophilic antioxidants i ... | 2008 | 26003372 |
| development of hen antihepatitis b antigen igy-based conjugate for elisa assay. | chicken antibodies have many advantages to the mammalian antibodies and have several important differences against mammalian igg with regard to their specificity and large-scale production. in this study, the production, purification, and hrp conjugation of polyclonal igy against hepatitis virus surface antigen (hbsag) were carried out. | 2015 | 26015926 |
| a colorimetric-based amplification system for proteinases including mmp2 and adam8. | we have developed a new amplification system for proteinases that is sensitive, simple, and inexpensive to run, exemplified by a horseradish peroxidase (hrp)-conjugated, dual mmp2 (matrix metalloproteinase 2) and adam8 (a disintegrin and metalloproteinase 8) peptide substrate assay presented herein. the hrp-conjugated substrate is attached to beads through a 6× histidine tag and then incubated with the target enzyme, cleaving the hrp reporter. this product is subsequently removed from the unreac ... | 2015 | 26026386 |
| dual-signal amplification strategy for ultrasensitive chemiluminescence detection of pdgf-bb in capillary electrophoresis. | many efforts have been made toward the achievement of high sensitivity in capillary electrophoresis coupled with chemiluminescence detection (ce-cl). this work describes a novel dual-signal amplification strategy for highly specific and ultrasensitive cl detection of human platelet-derived growth factor-bb (pdgf-bb) using both aptamer and horseradish peroxidase (hrp) modified gold nanoparticles (hrp-aunps-aptamer) as nanoprobes in ce. both aunps and hrp in the nanoprobes could amplify the cl sig ... | 2015 | 26031509 |
| development of a novel urine alzheimer-associated neuronal thread protein elisa kit and its potential use in the diagnosis of alzheimer's disease. | alzheimer's disease (ad) is an age-related chronic degenerative disease that damages the nervous system. a noninvasive and simple method for early detection of ad is extremely important for the diagnosis and prognosis of ad. thus, we aimed to develop an enzyme-linked immunosorbent assay (elisa) kit to detect urine alzheimer-associated neuronal thread protein (ad7c-ntp), and to evaluate its clinical value for the diagnosis of ad. | 2016 | 26037289 |
| mimicking enzymatic effects of cytochrome p450 by an efficient biosensor for in vitro detection of dna damage. | a novel biosensor for detecting dna damage induced by benzo(a)pyrene (bp) and its metabolite was presented in this work. the nafion-solubilized single wall carbon nanotubes-ionic liquid (swcnts-na-il) composite film was prepared and then horseradish peroxidase (hrp) and double-stranded dna were alternately assembled on the composite film by the layer-by-layer method. the biosensor was characterized by cyclic voltammetry (cv), electrochemical impedance spectroscopy (eis), differential pulse volta ... | 2015 | 26038105 |
| strong protection against ricin challenge induced by a novel modified ricin a-chain protein in mouse model. | ricin toxin (rt) is an extremely potent toxin derived from the castor bean plant. as a possible bioterrorist weapon, it was categorized as a level b agent in international society. with the growing awareness and concerns of the "white powder incident" in recent years, it is indispensable to develop an effective countermeasure against rt intoxication. in this study we used site-directed mutagenesis and polymerase chain reaction (pcr) techniques to modify the gene of ricin a-chain (rta). as a resu ... | 2015 | 26038805 |
| biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. | enhancing the robustness of functional biomacromolecules is a critical challenge in biotechnology, which if addressed would enhance their use in pharmaceuticals, chemical processing and biostorage. here we report a novel method, inspired by natural biomineralization processes, which provides unprecedented protection of biomacromolecules by encapsulating them within a class of porous materials termed metal-organic frameworks. we show that proteins, enzymes and dna rapidly induce the formation of ... | 2015 | 26041070 |
| amplified electrochemical hydrogen peroxide reduction based on hemin/g-quadruplex dnazyme as electrocatalyst at gold particles modified heated copper disk electrode. | a new gold particles modified heated copper disk electrode (au-hcude) with direct current was fabricated. the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme (hrp-dnazyme) was self-assembled on the heated electrode and resulted in a new biosensor denoted as hrp-dnazyme/au-hcude. by controlling the temperature of the surface of the electrode, the dramatic temperature effect on the electrocatalytic reduction of h2o2 at hrp-dnazyme/au-hcude sensing platform was demonstrated. this electr ... | 2015 | 26043314 |
| a comparison of three amperometric phenoloxidase-sonogel-carbon based biosensors for determination of polyphenols in beers. | three phenoloxidases based biosensors were successfully developed using as electrochemical transducer a new type of electrode recently developed by our group: the "sonogel-carbon electrode". the employed enzymes were trametes versicolor laccase (lac), mushroom tyrosinase (tyr), and horseradish peroxidase (hrp). immobilization step was accomplished by doping the electrode surface with a mixture of the individual enzyme and nafion ion exchanger as additive-protective. the biosensor responses, opti ... | 2008 | 26047296 |
| a new generation of flowerlike horseradish peroxides as a nanobiocatalyst for superior enzymatic activity. | although various supports including nanomaterials have been widely utilized as platforms for enzymes immobilization in order to enhance their catalytic activities, most of immobilized enzymes exhibited reduced activities compared to free enzymes. in this study, for the first time, we used iron ions (fe(2+)) and horseradish peroxidase (hrp) enzyme together to synthesize flowerlike hybrid nanostructures with greatly enhanced activity and stability and reported an explanation of the enhancements in ... | 2015 | 26047912 |
| sensitive electrochemical detection of salmonella with chitosan-gold nanoparticles composite film. | an ultrasensitive electrochemical immunosensor for detection of salmonella has been developed based on using high density gold nanoparticles (gnps) well dispersed in chitosan hydrogel and modified glassy carbon electrode. the composite film has been oxidized in nacl solution and used as a platform for the immobilization of capture antibody (ab1) for biorecognition. after incubation in salmonella suspension and horseradish peroxidase (hrp) conjugated secondary antibody (ab2) solution, a sandwich ... | 2015 | 26048833 |
| chemiluminescence immunoassay using magnetic nanoparticles with targeted inhibition for the determination of ochratoxin a. | in this work, a chemiluminescence (cl) immunoassay with targeted inhibition was developed for the determination of toxins in food products. for sample treatment, amine-functionalized magnetic nanoparticles (mnps) were synthesized to extract target molecules, and horseradish peroxidase (hrp) tagged on an antibody was used as a label for cl reaction. in particular, amine-targeted inhibition using aldehyde, i.e., specifically capping the amine with an alkyl group, was developed for a non-specific e ... | 2015 | 26048840 |
| dna transducer-triggered signal switch for visual colorimetric bioanalysis. | a simple and versatile colorimetric biosensor has been developed for sensitive and specific detection of a wide range of biomolecules, such as oligonucleotides and aptamer-recognized targets. combining the signal transducer and catalyzed hairpin assembly (cha)-based signal amplification, the target dna binds with the hairpin dna to form a new nucleic acid sequence and creates a toehold in the transducer for initiating the recycle amplification reaction of cha. the catalyzed assembly process prod ... | 2015 | 26060886 |
| enzyme-triggered tyramine-enzyme repeats on prussian blue-gold hybrid nanostructures for highly sensitive electrochemical immunoassay of tissue polypeptide antigen. | a novel sandwich-type electrochemical immunoassay with sensitivity enhancement was developed for quantitative detection of tissue polypeptide antigen (tpa) by coupling with target-induced tyramine signal amplification on prussian blue-gold hybrid nanostructures. the immunosensor was prepared through immobilizing anti-tpa capture antibody on a cleaned screen-printed carbon electrode (spce). prussian blue-gold hybrid nanostructures (pbgns) labeled with horseradish peroxidase (hrp) and detection an ... | 2015 | 26067328 |
| a highly sensitive and selective hydrogen peroxide biosensor based on gold nanoparticles and three-dimensional porous carbonized chicken eggshell membrane. | a sensitive and noble amperometric horseradish peroxidase (hrp) biosensor is fabricated via the deposition of gold nanoparticles (aunps) onto a three-dimensional (3d) porous carbonized chicken eggshell membrane (cesm). due to the synergistic effects of the unique porous carbon architecture and well-distributed aunps, the enzyme-modified electrode shows an excellent electrochemical redox behavior. compared with bare glass carbon electrode (gce), the cathodic peak current of the enzymatic electrod ... | 2015 | 26069960 |
| assessment of retinal ganglion cell damage in glaucomatous optic neuropathy: axon transport, injury and soma loss. | glaucoma is a disease characterized by progressive axonal pathology and death of retinal ganglion cells (rgcs), which causes structural changes in the optic nerve head and irreversible vision loss. several experimental models of glaucomatous optic neuropathy (gon) have been developed, primarily in non-human primates and, more recently and commonly, in rodents. these models provide important research tools to study the mechanisms underlying glaucomatous damage. moreover, experimental gon provides ... | 2015 | 26070986 |
| canine distemper virus infection among wildlife before and after the epidemic. | in 2007-2008, a canine distemper virus (cdv) epidemic occurred among wild animals in wakayama prefecture, japan, and many mammals, including the wild boar and deer, were infected. in this study, cdv prevalence among wild animals was surveyed before and after the epidemic. at first, an enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase-conjugated protein a/g was established to detect cdv antibodies in many mammalian species. this established elisa was available for testing dogs ... | 2015 | 26074342 |
| a novel strategy to improve the sensitivity of antibiotics determination based on bioelectrocatalysis at molecularly imprinted polymer film electrodes. | a new strategy for the sensitive detection of kanamycin (ka) and other antibiotics based on molecularly imprinted polymer (mip) and bioelectrocatalysis was developed in the present study. the ka-polypyrrole mip films were electropolymerized on the surface of pyrolytic graphite (pg) electrodes, with pyrrole (py) serving as the monomer and ka as the template. because ka is electro-inactive, electroactive k3[fe(cn)6] was used as the probe in the cyclic voltammetric (cv) measurements. the difference ... | 2015 | 26079673 |
| physicochemical changes of few-layer graphene in peroxidase-catalyzed reactions: characterization and potential ecological effects. | the environmental implications of graphene have received much attention, however, little is known about how graphene affects or may be affected by the enzymatic reactions that are critically involved in natural organic matter transformation processes. we conducted experiments to examine the role of few-layer graphene (flg) in the reaction system of tetrabromobisphenol a (tbbpa) mediated by horseradish peroxidase (hrp). we found that tbbpa was transformed by hrp into two products that were likely ... | 2015 | 26086574 |
| the development of an electrochemical immunosensor using a thiol aromatic aldehyde and pamam-functionalized fe3o4@au nanoparticles. | in this work, a novel thiol aromatic aldehyde was synthesized. it can be used as a substrate to directly immobilize antibodies on a gold electrode, for which no additional chemical cross-linker is required. it was also applied as a linker to prepare fe3o4@au/pamam/ab2-horseradish peroxidase bioconjugates, which introduced multiple enzymes onto a sensing interface owing to the high surface-to-volume ratio of fe3o4@au nanoparticles and many functional groups of the poly(amidoamine) dendrimer (pama ... | 2015 | 26087149 |
| increased sensitivity of hiv-1 p24 elisa using a photochemical signal amplification system. | in this study we describe a photochemical signal amplification method (psam) for increasing of the sensitivity of enzyme-linked immunosorbent assay (elisa) for determination of hiv-1 p24 antigen. the photochemical signal amplification method is based on an autocatalytic photochemical reaction of a horseradish peroxidase (hrp) substrate, orthophenylenediamine (opd). | 2015 | 26090753 |
| phenylbutazone oxidation via cu,zn-sod peroxidase activity: an epr study. | we investigated the effect of cu,zn-superoxide dismutase (cu,zn-sod)-peroxidase activity on the oxidation of the nonsteroidal anti-inflammatory drug phenylbutazone (pbz). we utilized electron paramagnetic resonance (epr) spectroscopy to detect free radical intermediates of pbz, uv-vis spectrophotometry to monitor pbz oxidation, oxygen analysis to determine the involvement of c-centered radicals, and lc/ms to determine the resulting metabolites. using epr spectroscopy and spin-trapping with 5,5-d ... | 2015 | 26090772 |
| a new colorimetric platform for ultrasensitive detection of protein and cancer cells based on the assembly of nucleic acids and proteins. | an amplified colorimetric method has been developed for the detection of protein and cancer cells based on the assembly of nucleic acids and proteins for the first time. in this process, the assembly of nucleic acids was triggered by a biotinylated dna strand after a sandwich immunoreaction. the biotinylated dna strand and sandwich immunocomplex were connected by streptavidin. then, the assembly of biotinylated bovine serum albumin (biotin-bsa) and streptavidin-horseradish peroxidase (sa-hrp) oc ... | 2015 | 26092332 |
| a novel ofet-based biosensor for the selective and sensitive detection of lactate levels. | biosensors based on organic field effect transistors (ofets) are one of the more promising device applications in organic electronics. however, ofet-based biosensors are still in their early stages of development compared to other electrochemical biosensors. this study is the first to report on an extended-gate type organic field effect transistor (ofet) for lactate detection in aqueous media. here, the extended-gate electrode of the ofet was modified with layers of a lactate oxidase and a horse ... | 2015 | 26101795 |
| drosophila sperm surface alpha-l-fucosidase interacts with the egg coats through its core fucose residues. | sperm-oocyte interaction during fertilization is multiphasic, with multicomponent events, taking place between egg's glycoproteins and sperm surface receptors. protein-carbohydrate complementarities in gamete recognition have observed in cases throughout the whole evolutionary scale. sperm-associated α-l-fucosidases have been identified in various organisms. their wide distribution and known properties reflect the hypothesis that fucose and α-l-fucosidases have fundamental function(s) during gam ... | 2015 | 26101846 |
| peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces. | postsurgical peritoneal adhesion is a serious surgical complication. in situ hydrogel formation on the surface of tissues, which will develop adhesions, is a recent feasible approach to prevent peritoneal adhesion. here, we report on-tissue surface formation of a hyaluronic acid-based hydrogel by administration of a pre-hydrogel aqueous solution. the hydrogelation was initiated by contact with body fluid containing glucose on tissue surfaces. during the hydrogelation, a hyaluronic acid derivativ ... | 2015 | 26102338 |
| removal of sulfadimethoxine in soil mediated by extracellular oxidoreductases. | sulfadimethoxine (sdm) is an antibiotic commonly used in concentrated animal feeding operations and released into the environment via manure application on agricultural lands. transformation of antibiotics in soil impacts the likelihood of their entry to water bodies, uptake by plants, and thus their effect on terrestrial and aquatic organisms. we conducted experiments to incubate sdm in a sandy loam soil in the presence of humification enzymes commonly found in natural soil, laccase, horseradis ... | 2015 | 26104901 |
| pp131. regulation of heme oxygenase/carbon monoxide (ho/co) and l-arginine/nitric oxide (no) pathways by human chorionic gonadotropin (hcg) in human fetal endothelium. | ho/co and l-arginine/no pathways are critical regulators of vascular function in early human pregnancy. ho is the rate-limiting enzyme in heme catabolism leading to the formation of biliverdin, which is subsequently reduced to bilirubin by biliverdin reductase, carbon monoxide, and free iron. ho-1, the inducible isoform of the enzyme, is essential to promote placental function and fetal development (1). ho-1 is also required to maintain nitric oxide synthesis (2). human chorionic gonadotropin (h ... | 2012 | 26105453 |
| novel chemiluminescent imaging microtiter plates for high-throughput detection of multiple serum biomarkers related to down's syndrome via soybean peroxidase as label enzyme. | novel chemiluminescent (cl) imaging microtiter plates with high-throughput, low-cost, and simple operation for detection of four biomarkers related to down's syndrome screening were developed and evaluated. to enhance the sensitivity of cl immunosensing, soybean peroxidase (sbp) was used instead of horseradish peroxide (hrp) as a label enzyme. the microtiter plates were fabricated by simultaneously immobilizing four capture monoclonal antibodies, anti-inhibin-a, anti-unconjugated oestriol (anti- ... | 2015 | 26105511 |
| atp-enhanced peroxidase-like activity of gold nanoparticles. | gold nanoparticles (aunps) are known to possess intrinsic biological peroxidase-like activity that has applications in development of numerous biosensors. the reactivity of the au atoms at the surface of aunps is critical to the performance of such biosensors, yet little is known about the effect of biomolecules and ions on the peroxidase-like activity. in this work, the effect of atp and other biologically relevant molecules and ions over peroxidase-like activity of aunps are described. contrar ... | 2015 | 26111515 |
| enzyme-mediated hyaluronic acid-tyramine hydrogels for the propagation of human embryonic stem cells in 3d. | the propagation of human embryonic stem cells (hescs) in three-dimensional (3d) scaffolds facilitates the cell expansion process and supplies pluripotent cells of high quality for broad-spectrum applications in regenerative medicine. herein, we report an enzyme-mediated hyaluronic acid-tyramine (ha-tyr) hydrogel that encapsulated and propagated hescs in 3d. ha-tyr hydrogels were formed by crosslinking the tyramine moieties with horseradish peroxidase (hrp) and hydrogen peroxide (h2o2). by changi ... | 2015 | 26112373 |
| iron impurities as the active sites for peroxidase-like catalytic reaction on graphene and its derivatives. | we established four kinds of good dispersing systems of graphene and its derivatives with different structural characteristics to estimate their peroxidase-like activity. besides graphene oxide (go), it is demonstrated that defect-free graphene, low-oxygen graphene, and iron(iii)-doped graphene oxide (go-fe) are all capable of h2o2 activation to oxidize peroxidase substrates. as for defect-free graphene, the dispersibility in reaction medium exerts great impact on its catalytic activity and our ... | 2015 | 26115555 |
| in vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels. | three-dimensional (3d) hydrogel-based stem cell therapies contribute to enhanced therapeutic efficacy in treating diseases, and determining the optimal mechanical strength of the hydrogel in vivo is important for therapeutic success. we evaluated the proliferation of human neural stem cells incorporated within in situ-forming hydrogels and compared the effect of hydrogels with different elastic properties in cell/hydrogel-xenografted mice. | 2014 | 26116122 |
| one-pot synthesis of porphyrin functionalized γ-fe2o3 nanocomposites as peroxidase mimics for h2o2 and glucose detection. | meso-tetrakis(4-carboxyphenyl)-porphyrin-functionalized γ-fe2o3 nanoparticles (h2tcpp-γ-fe2o3) were successfully prepared by one-pot method under hydrothermal conditions and were found to possess intrinsic peroxidase-like activity. the h2tcpp-γ-fe2o3 nanocomposites can catalytically oxidize peroxidase substrate 3,3',5,5'-tetramethylbenzidine (tmb) in the presence of h2o2 to produce a blue color reaction, which can be easily observed by the naked eye. furthermore, kinetic studies indicate that th ... | 2015 | 26117755 |
| oxidation pathways underlying the pro-oxidant effects of apigenin. | apigenin, a natural flavone, is emerging as a promising compound for the treatment of several diseases. one of the hallmarks of apigenin is the generation of intracellular reactive oxygen species (ros), as judged by the oxidation of reduced dichlorofluorescein derivatives seen in many cell types. this study aimed to reveal some mechanisms by which apigenin can be oxidized and how apigenin-derived radicals affect the oxidation of 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein (h(2)dcf), ... | 2015 | 26119779 |
| combined experimental and theoretical study on the removal of pollutant compounds by peroxidases: affinity and reactivity toward a bioremediation catalyst. | water pollution is a significant and growing problem throughout the world, especially in developing countries. in order to minimize environmental problems, catalysts have increasingly been designed to remove pollutants from the water. in an attempt to innovate by the creation of new low-cost alternatives to efficiently remove pollutants, the enzymatic treatment has been intensely studied for this purpose. reactions catalyzed by enzymes are able to perform specific treatments, commonly with high ... | 2016 | 26130457 |
| eicosapentaenoic acid enhances heatstroke-impaired intestinal epithelial barrier function in rats. | dysfunction of the intestinal barrier plays an important role in the pathological process of heatstroke. omega-3 (or n-3) polyunsaturated fatty acids, including eicosapentaenoic acid (epa) and docosahexaenoic acid (dha), help protect the intestinal mucosal barrier. this study assessed if pretreating rats with epa or dha could alleviate heat stress-induced damage to the intestinal barrier caused by experimental heatstroke. | 2015 | 26131715 |
| electrochemical control of rapid bioorthogonal tetrazine ligations for selective functionalization of microelectrodes. | we demonstrate that bioorthogonal tetrazine ligations can be utilized to rapidly modify electrode surfaces, both with redox probes and enzymes. furthermore, we show that the redox-active nature of 1,2,4,5-tetrazines can be exploited to gain electrochemical control over surface modification. to our knowledge this is the first demonstration of controlling a tetrazine ligation by changing the redox state of one of the reactants. we utilize the redox-switchable feature of tetrazine ligations for the ... | 2015 | 26132207 |
| a novel and versatile nanomachine for ultrasensitive and specific detection of micrornas based on molecular beacon initiated strand displacement amplification coupled with catalytic hairpin assembly with dnazyme formation. | micrornas are small regulatory molecules that can be used as potential biomarkers of clinical diagnosis, and efforts have been directed towards the development of a simple, rapid, and sequence-selective analysis of micrornas. here, we report a simple and versatile colorimetric strategy for ultrasensitive and specific determination of micrornas based on molecular beacon initiated strand displacement amplification (sda) and catalytic hairpin assembly (cha) with dnazyme formation. the presence of t ... | 2015 | 26134555 |
| triple quenching of a novel self-enhanced ru(ii) complex by hemin/g-quadruplex dnazymes and its potential application to quantitative protein detection. | herein, a novel "on-off" electrochemiluminescence (ecl) aptasensor for highly sensitive determination of thrombin has been constructed based on the triple quenching of the effect of hemin/g-quadruplex dnazymes upon the ru(ii) complex-based ecl system. first, a strong initial ecl signal was achieved by the dual amplification strategies of (i) intramolecular coreaction of a self-enhanced ru(ii)-based molecule (ptca-pei-ru(ii)) and (ii) intermolecular coreaction between ptca-pei-ru(ii) and nicotina ... | 2015 | 26135920 |
| rhinovirus-induced vp1-specific antibodies are group-specific and associated with severity of respiratory symptoms. | rhinoviruses (rvs) are a major cause of common colds and induce exacerbations of asthma and chronic inflammatory lung diseases. | 2015 | 26137535 |
| nonstripping "rainbow" and multiple antigen detection (mad) western blotting. | a variation of immunoblotting method (the "rainbow western"), permits sequential detection of multiple antigens (mad) on a single protein blot without stripping off prior antibodies. because no stripping is involved, immobilized proteins are not lost from the membrane, thus allowing for multiple reprobings of the same membrane with different primary antibodies (≥12), retaining strong signal intensities for all sequential antibody probings. the procedure utilizes horseradish peroxidase (hrpase)-b ... | 2015 | 26139277 |
| other notable methods of membrane protein detection: a brief review. | several techniques have been employed to detect proteins on membranes. these include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, "coupled" probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay, and use of congo red dye (a cosmetic color named alta), pro-q emerald 488 dye, or amine-reactive dye in combination with alkaline ... | 2015 | 26139283 |
| ultrasensitive strategy based on ptpd nanodendrite/nano-flower-like@go signal amplification for the detection of long non-coding rna. | highly up-regulated in liver cancer (hulc) is a novel promising noninvasive biomarker for hepatocellular carcinoma (hcc), which is a kind of long non-coding rnas (lncrnas). but traditional methods limited hulc clinical detection for ownself drawbacks. development a new hulc detection approach is urgent and necessary. electrochemical nucleic acid sensor based on different signal amplification strategies with high sensitivity, fast, simple, and convenient, may solve this problem. herein, we propos ... | 2015 | 26143461 |
| carbohydrate derivative-functionalized biosensing toward highly sensitive electrochemical detection of cell surface glycan expression as cancer biomarker. | accurate and highly sensitive detection of glycan expression on cell surface is extremely important for cancer diagnosis and therapy. herein, a carbohydrate derivative-functionalized biosensor was developed for electrochemical detection of the expression level of cell surface glycan (mannose used as model). thiomannosyl dimer was synthesized to design the thiomannosyl-functionalized biosensor by direct and rapid one-step protocols. the biosensing surface-confined mannose could effectively mimic ... | 2015 | 26143470 |
| propagation of human ips cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase. | cell encapsulation has been investigated as a bioproduction system in the biomedical and pharmaceutical fields. we encaps-ulated human induced pluripotent stem (hips) cells in duplex microcapsules prepared from an alginate derivative possessing phenolic hydroxyl moieties, in a single-step procedure based on two competing enzymatic reactions catalyzed by horseradish peroxidase (hrp) and catalase. the encapsulated cells maintained 91.4% viability and proliferated to fill the microcapsules followin ... | 2016 | 26148179 |
| high throughput automated colorimetric method for the screening of l-lactic acid producing microorganisms. | lactic acid is a valuable and fully degradable organic acid with promising applications in poly-lactic acid production (taskila s and ojamo, 2013 [1]). despite their efficiency, the cost of the current lactic acid bio-processes is still an obstacle to this application (miller et al., 2011 [2]). to ameliorate lactic acid producing strains, researchers are using mutations and metabolic engineering techniques, as well as medium optimization. all these studies necessitate a good and high throughput ... | 2014 | 26150961 |
| clinical relevance of mir-mediated hla-g regulation and the associated immune cell infiltration in renal cell carcinoma. | in human tumors of distinct origin including renal cell carcinoma (rcc), the non-classical human leukocyte antigen g (hla-g) is frequently expressed, thereby inhibiting the cytotoxic activity of t and natural killer (nk) cells. recent studies demonstrated a strong post-transcriptional gene regulation of the hla-g by mir-152, -148a, -148b and -133a. standard methods were applied to characterize the expression and function of hla-g, hla-g-regulatory micrornas (mirs) and the immune cell infiltratio ... | 2015 | 26155421 |
| bioconjugation of antibodies to horseradish peroxidase (hrp). | the bioconjugation of an antibody to an enzymatic reporter such as horseradish peroxidase (hrp) affords an effective mechanism by which immunoassay detection of a target antigen can be achieved. the use of heterobifunctional cross-linkers to covalently link antibodies to hrp provides a simple and convenient means to maintain antibody affinity while imparting a functional reporter used for antigen detection. in this chapter, we describe a process by which sulfo-smcc is used to generate a stable m ... | 2015 | 26160562 |
| direct elisa. | first described by engvall and perlmann, the enzyme-linked immunosorbent assay (elisa) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. besides routine laboratory usage, elisa has been utilized in medical field and food industry as diagnostic and quality control tools. traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with increase in automation. depending on the antigen ... | 2015 | 26160564 |
| tyramide signal amplification for immunofluorescent enhancement. | enzyme-linked signal amplification is a key technique used to enhance the immunohistochemical detection of protein, mrna, and other molecular species. tyramide signal amplification (tsa) is based on a catalytic reporter deposit in close vicinity to the epitope of interest. the advantages of this technique are its simplicity, enhanced sensitivity, high specificity, and compatibility with modern multi-label fluorescent microscopy. here, we describe the use of a tsa kit to increase the signal of en ... | 2015 | 26160574 |
| evaluation of dye compounds' decolorization capacity of selected h. haematococca and t. harzianum strains by principal component analysis (pca). | the selected strains of microscopic fungi, haematonectria haematococca (bwiii43, k37) and trichoderma harzianum (bsiii33), decolorized the following monoathraquinone dyes with different efficiency: 0.03 % alizarin blue black b, 0.01 % carminic acid, 0.01 % poly r-478, and 0.2 % post-industrial lignin. the most effective was the removal of 0.03 % alizarin blue black b (50-60 %) and 0.01 % carminic acid (55-85 %). the principal component analysis (pca) method was applied to determine the main enzy ... | 2017 | 26160991 |
| electrochemical aptasensor for the detection of adenosine by using pdcu@mwcnts-supported bienzymes as labels. | a highly sensitive electrochemical adenosine aptasensor was fabricated by covalently immobilizing 3'-nh2-terminated capture probe (ssdna1) and thionine (th) on au-gs modified glassy carbon electrode. 3'-sh-terminated adenosine aptamer (ssdna2) was adsorbed onto palladium/copper alloyed supported on mwcnts (pdcu@mwcnts)-conjugated multiple bienzymes, glucose oxidase (gox), and horseradish peroxidase (hrp) (ssdna2/pdcu@mwcnts/hrp/gox). then, it was immobilized onto the electrode surface through th ... | 2015 | 26164010 |
| a comparison between the oxidation with laccase and horseradish peroxidase for triclosan conversion. | triclosan is a broad-spectrum biocide used in personal-care products that is suspected to be linked to the emergence of antibiotic-resistant bacteria. in the present work, the enzymes horseradish peroxidase and laccase from trametes versicolor were evaluated for the conversion of triclosan in an aqueous matrix. the removal of antibacterial activity by the enzymatic processes was evaluated by an assay based on the growth inhibition of escherichia coli k12. the horseradish peroxidase (hrp) process ... | 2016 | 26165135 |
| an extended-gate type organic fet based biosensor for detecting biogenic amines in aqueous solution. | this study is the first to report on the detection of biogenic amines in an aqueous solution using an organic field-effect transistor (ofet) device with an extended gate electrode modified with a layer of diamine oxidase and a horseradish peroxidase osmium-redox polymer. the limit of detection (lod) for histamine was estimated to be 1.2 μm. these results reveal that extended-gate type ofet devices are highly suitable enzyme-based biosensors for the detection of biogenic amine levels. | 2015 | 26165298 |
| highly sensitive chemiluminescent detection of lead ion based on its displacement of potassium in g-quadruplex dnazyme. | a simple and highly sensitive method for detecting lead ion (pb(2+)) in biosamples was developed based on its displacement of potassium in g-quadruplex dnazyme, which can catalyze the luminol-h2o2 chemiluminescence (cl) reaction. by introducing a g-rich dna sequence, ps2.m, which can fold into a g-quadruplex when binding with hemin in the presence of k(+) and act as a superior horseradish peroxidase (hrp) mimicking-enzyme, we found this dnazyme can effectively catalyze the h2o2-mediated oxidatio ... | 2015 | 26165488 |
| restriction site free cloning (rsfc) plasmid family for seamless, sequence independent cloning in pichia pastoris. | tagging proteins is a standard method facilitating protein detection, purification or targeting. when tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. ideally, multiple tags and positions are tested which however complicates molecular cloning and expression vector generation. in conventional cloning, tags are either added on pcr primers (requiring a distinct primer and pcr prod ... | 2015 | 26169367 |
| propofol injection combined with bone marrow mesenchymal stem cell transplantation better improves electrophysiological function in the hindlimb of rats with spinal cord injury than monotherapy. | the repair effects of bone marrow mesenchymal stem cell transplantation on nervous system damage are not satisfactory. propofol has been shown to protect against spinal cord injury. therefore, this study sought to explore the therapeutic effects of their combination on spinal cord injury. rat models of spinal cord injury were established using the weight drop method. rats were subjected to bone marrow mesenchymal stem cell transplantation via tail vein injection and/or propofol injection via tai ... | 2015 | 26170827 |
| artificial peroxidase/oxidase multiple enzyme system based on supramolecular hydrogel and its application as a biocatalyst for cascade reactions. | inspired by delicate structures and multiple functions of natural multiple enzyme architectures such as peroxisomes, we constructed an artificial multiple enzyme system by coencapsulation of glucose oxidases (gox) and artificial peroxidases in a supramolecular hydrogel. the artificial peroxidase was a functional complex micelle, which was prepared by the self-assembly of diblock copolymer and hemin. compared with catalase or horseradish peroxidase (hrp), the functional micelle exhibited comparab ... | 2015 | 26173996 |
| carbon nanotubes labeled with aptamer and horseradish peroxidase as a probe for highly sensitive protein biosensing by postelectropolymerization of insoluble precipitates on electrodes. | carbon nanotubes (cnts) labeled with aptamer and horseradish peroxidase (hrp) were used as a probe to amplify the impedimetric sensing of the aptamer-protein (with thrombin as the model) interaction. the hrp-biocatalyzed oxidation of 3,3-diaminobenzidine (dab) in the presence of h2o2 and the postelectropolymerization of insoluble precipitates produced on the electrode supports were used as a signal amplification route for the sensing process. thrombin was sensed by aptamer 1 immobilized on a gla ... | 2015 | 26176595 |
| enzymatic hydrolysate-induced displacement reaction with multifunctional silica beads doped with horseradish peroxidase-thionine conjugate for ultrasensitive electrochemical immunoassay. | a novel (invertase) enzymatic hydrolysate-triggered displacement reaction strategy with multifunctional silica beads, doped with horseradish peroxidase-thionine (hrp-thi) conjugate, was developed for competitive-type electrochemical immunoassay of small molecular aflatoxin b1 (afb1). the competitive-type displacement reaction was carried out on the basis of the affinity difference between enzymatic hydrolysate (glucose) and its analogue (dextran) for concanavalin a (con a) binding sites. initial ... | 2015 | 26181887 |
| development of a carbon dot (c-dot)-linked immunosorbent assay for the detection of human α-fetoprotein. | a sensitive, selective, environmentally friendly, high-throughput, well-plate-based immunosorbent assay was developed to detect human α-fetoprotein (afp) using carbon dots (c-dots). highly fluorescent c-dots were synthesized using a one-step hydrothermal reaction, with citric acid serving as the carbon source and ethylene diamine acting as the nitrogen source. the reaction conditions were optimized to obtain the desired surface functionality. then, the c-dots were used to label one member of the ... | 2015 | 26186018 |
| glucose biosensor based on gox/hrp bienzyme at liquid-crystal/aqueous interface. | glucose oxidase (gox) and horseradish peroxidase (hrp) were co-immobilized to the polyacrylicacid block of a poly(acrylicacid-b-4-cyanobiphenyl-4'-undecylacrylate) (paa-b-lcp) copolymer in water. paa-b-lcp was strongly anchored by the lcp block in 4-cyano-4'-pentylbiphenyl (5cb) which was contained in a transmission electron microscope (tem) grid for glucose detection. the optimal conditions for the performance of the tem grid glucose biosensor were studied in terms of the activity and stability ... | 2015 | 26196711 |
| protein microarray with horseradish peroxidase chemiluminescence for quantification of serum α-fetoprotein. | to develop and evaluate a protein microarray assay with horseradish peroxidase (hrp) chemiluminescence for quantification of α-fetoprotein (afp) in serum from patients with hepatocellular carcinoma (hcc). | 2015 | 26198141 |
| designing hydrophobically modified polysaccharide derivatives for highly efficient enzyme immobilization. | in this contribution, a hydrophobically modified polysaccharide derivative is synthesized in an eco-friendly solvent water by conjugation of benzylamine with the backbone of the biopolymer. owing to the presence of aromatic moieties, the resulting water-soluble polysaccharide derivative self-assembles spontaneously and selectively from solution on the surface of nanometric thin films and sheets of polystyrene (ps). the synthetic polymer modified in this way bears a biocompatible nanolayer suitab ... | 2015 | 26200740 |
| dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase. | this study describes a novel electrochemical immunosensor to amplify the electrochemiluminescence (ecl) signal for the ultrasensitive detection of salbutamol (sal) using quantum dots (qds) and gold nanoparticle (aunp) conjugated horseradish peroxidase (hrp). the electrochemical detection was based on the hrp catalyzed consumption of self-produced h2o2, which has been extensively used as a co-reactant of qds, by o-phenylenediamine (opd). the enzymatic reaction rate is proportional to the amount o ... | 2015 | 26200776 |
| a afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats. | a afferent fibers have been reported to participate in the development of the central sensitization induced by inflammation and injuries. current evidence suggests that myofascial trigger points (mtrps) induce central sensitization in the related spinal dorsal horn, and clinical studies indicate that a fibers are associated with pain behavior. because most of these clinical studies applied behavioral indexes, objective evidence is needed. additionally, mtrp-related neurons in dorsal root ganglia ... | 2015 | 26210690 |
| multienzyme inkjet printed 2d arrays. | the use of printing to produce 2d arrays is well established, and should be relatively facile to adapt for the purpose of printing biomaterials; however, very few studies have been published using enzyme solutions as inks. among the printing technologies, inkjet printing is highly suitable for printing biomaterials and specifically enzymes, as it offers many advantages. formulation of the inkjet inks is relatively simple and can be adjusted to a variety of biomaterials, while providing nonharmfu ... | 2015 | 26214072 |
| rapid and sensitive identification of marine bacteria by an improved in situ dna hybridization chain reaction (quickhcr-fish). | catalyzed reporter deposition-fluorescence in situ hybridization (card-fish) with rrna-targeted oligonucleotide probes has significantly improved the identification of microorganisms in various environmental samples. however, one of the major constraints of card-fish is the low probe penetration due to the high molecular weight of the horseradish peroxidase (hrp) label. recently, this limitation has been overcome by a novel signal amplification approach termed in situ dna-hybridization chain rea ... | 2015 | 26215142 |
| expression and clinical implication of cyclooxygenase-2 and e-cadherin in oral squamous cell carcinomas. | epithelial-mesenchymal transition (emt) and angiogenesis are crucial events for development of aggressive and often fatal oral squamous cell carcinomas (osccs). both promote cancer progression and metastasis development, but while the former induces the loss of e-cadherin expression and, hence cadherin switching; the latter produces haematic blood vessel neo-formation and contribute to oscc cell growth, tumor mass development, and dissemination. cyclooxygenase-2 (cox-2) has an important role, no ... | 2015 | 26218314 |
| microfabrication of photo-cross-linked hyaluronan hydrogels by single- and two-photon tyramine oxidation. | photo-cross-linking of tyramine-substituted hyaluronan (ha-tyr) hydrogels is demonstrated for the first time. ha-tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hmscs) with high viability. macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2d and 3d microfabrication. different degrees of cross-linking induce different sw ... | 2015 | 26222128 |
| optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles. | a simple optimization method of immobilization of avidin on magnetic nanoparticles (mnps)' surface was proposed in this study. the avidin-immobilized mnps were then developed and used to immobilize a model enzyme [horseradish peroxidase (hrp)]. the loading capacity (lc) and activity of avidin-immobilized mnps were optimized through selecting the most appropriate nanoparticle's size and shape, glutaraldehyde concentration, cross-linking reaction time, ultrasonic processing time, and initial conce ... | 2015 | 26224655 |
| studies on enzymatic oxidation of 3',4'-dihydroxy-l-phenylalanine to dopachrome using kinetic isotope effect methods. | we report the studies on the mechanism of oxidation of 3',4'-dihydroxy-l-phenylalanine (l-dopa) to neurotoxic dopachrome catalyzed by enzyme horseradish peroxidase (ec 1.11.1.7) using the kinetic (kie), and solvent (sie), isotope effect methods. for kinetic studies two specifically deuterated isotopomers: [2',5',6'-(2)h3]-l-dopa was synthesized by the acid catalyzed isotopic exchange between native l-dopa and heavy water, and [5'-(2)h]-l-dopa was synthesized in two step reaction. the first step ... | 2017 | 26224961 |
| bioinorganic nanocomposite hydrogels formed by hrp-gox-cascade-catalyzed polymerization and exfoliation of the layered composites. | the mild preparation of multifunctional nanocomposite hydrogels is of great importance for practical applications. we report that bioinorganic nanocomposite hydrogels, with calcium niobate nanosheets as cross-linkers, can be prepared by dual-enzyme-triggered polymerization and exfoliation of the layered composite. the layered hrp/calcium niobate composites (hrp=horseradish peroxidase) are formed by the assembly of the calcium niobate nanosheets with hrp. the dual-enzyme-triggered polymerization ... | 2015 | 26230284 |
| highly chemiluminescent graphene oxide hybrids bifunctionalized by n-(aminobutyl)-n-(ethylisoluminol)/horseradish peroxidase and sensitive sensing of hydrogen peroxide. | n-aminobutyl-n-ethylisoluminol and horseradish peroxidase bifunctionalized graphene oxide hybrids (abei-go@hrp) were prepared through a facile and green strategy for the first time. the hybrids exhibited excellent chemiluminescence (cl) activity over a wide range of ph from 6.1 to 13.0 when reacted with h2o2, whereas abei functionalized go had no cl emission at neutral ph and showed more than 2 orders of magnitude lower cl intensity than abei-go@hrp at ph 13.0. such strong cl emission from abei- ... | 2015 | 26230962 |
| mechanistic analysis of ultrasound assisted enzymatic desulfurization of liquid fuels using horseradish peroxidase. | this study has attempted to gain physical insight into ultrasound-assisted enzymatic desulfurization using system comprising horseradish peroxidase enzyme and dibenzothiophene (dbt). desulfurization pathway (comprising dbt-sulfoxide and dbt-sulfone as intermediates and 4-methoxy benzoic acid as final product) has been established with gc-ms analysis. intrinsic fluorescence and circular dichroism spectra of ultrasound-treated enzyme reveal conformational changes in secondary structure (reduction ... | 2015 | 26231128 |
| a repeatable assembling and disassembling electrochemical aptamer cytosensor for ultrasensitive and highly selective detection of human liver cancer cells. | in this work, a repeatable assembling and disassembling electrochemical aptamer cytosensor was proposed for the sensitive detection of human liver hepatocellular carcinoma cells (hepg2) based on a dual recognition and signal amplification strategy. a high-affinity thiolated tls11a aptamer, covalently attached to a gold electrode through au-thiol interactions, was adopted to recognize and capture the target hepg2 cells. meanwhile, the g-quadruplex/hemin/aptamer and horseradish peroxidase (hrp) mo ... | 2015 | 26231902 |
| branched zinc oxide nanorods arrays modified paper electrode for electrochemical immunosensing by combining biocatalytic precipitation reaction and competitive immunoassay mode. | branched zinc oxide nanorods (bzr) arrays, an array with high charge carries collection efficiency and specific surface area, are grown on the reduced graphene oxide-paper working electrode for the first time to construct a paper-based electrochemical (ec) immunosensor. typically, the bzr are fabricated via a simple hydrothermal process, which can provide abundant sites for antibodies loading. by combining the large surface area of porous zinc oxide (pzs) and good biocompatibility of gold nanopa ... | 2015 | 26232677 |
| [laminar location of neurons providing interhemispheric connections in the visual cortex in cats with impairments of binocular vision]. | the distributio of cells in the visual cortical layers of intact cats (n=7) and cats with experimentally induced strabismus (n=10) and monocular deprivation (n=5) was studied after microiontophoretic injection of horseradish peroxidase into the ocular-dominance columns in areas 17, 18 and the transition zone 17/18. it was found that in cats with impaired binocular vision, the callosal cells were located deeper in layers of i/ii, and higher - in layer iv, as compared to those in intact cats. also ... | 2015 | 26234033 |
| carboxylic-group-functionalized single-walled carbon nanohorns as peroxidase mimetics and their application to glucose detection. | carboxylic-group-functionalized single-walled carbon nanohorns (swcnhs-cooh) have been found to possess peroxidase-like activity for the first time. similar to natural peroxidase, swcnhs-cooh can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by h2o2 to produce a blue color solution. compared with horseradish peroxidase, swcnhs-cooh exhibit higher activity and stability under harsh reaction conditions. the catalytic activity of swcnhs-cooh depends on the concentration of h2o2. a colori ... | 2015 | 26247806 |
| [establishment and evaluation of methods for determinating cystic fibrosis transmembrane conductance regulator quantitatively]. | to establish and evaluate a ba-elisa method for the quantitative detection of cystic fibrosis transmembrane conductance regulator (cftr) protein. | 2015 | 26248424 |
| a new label-free strategy for a highly efficient chemiluminescence immunoassay. | a new label-free chemiluminescence (cl) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. the specific immunoreaction on the interface effectively inhibits the enzymatic cl reaction, thus causing a decrease in cl signals. | 2015 | 26248771 |
| label-free real-time quantification of enzyme levels by interferometric spectroscopy combined with gelatin-modified nanoporous anodic alumina photonic films. | herein, we present an interferometric sensor based on the combination of chemically functionalized nanoporous anodic alumina photonic films (naa-pfs) and reflectometric interference spectroscopy (rifs) aimed to detect trace levels of enzymes by selective digestion of gelatin. the fabrication and sensing performance of the proposed sensor were characterized in real-time by estimating the changes in effective optical thickness (i.e., sensing principle) of gelatin-modified naa-pfs (i.e., sensing el ... | 2015 | 26259031 |
| hyaluronidase-incorporated hyaluronic acid-tyramine hydrogels for the sustained release of trastuzumab. | we developed an injectable hydrogel system for the sustained release of protein drugs that incorporated both protein drugs and hyaluronidase. trastuzumab and hyaluronidase were incorporated in hydrogels composed of hyaluronic acid-tyramine (ha-tyr) conjugates through the enzymatic crosslinking utilizing hydrogen peroxide (h2o2) and horseradish peroxidase (hrp). through electrostatic interactions with the ha, trastuzumab was retained in the hydrogel to minimize its burst release. hyaluronidase wa ... | 2015 | 26260452 |
| s1 nerve is the most efficient nerve rootlet innervating the anal canal and rectum in rats. | autonomic and somatic components participate in the defecation process in mammals, combining signals from the brainstem and forebrain. the innervation pattern involved in micturition in rats has been well studied, while defecation has been less studied. the aim of the present study was to identify the most important sensory and motor nerves of the anal canal and rectum involved in defecation. the amplitudes of evoked potential of the anal canal and rectum were higher when l6 and s1 ventral rootl ... | 2015 | 26260583 |
| structure-activity relationship study on isothiocyanates: comparison of trpa1-activating ability between allyl isothiocyanate and specific flavor components of wasabi, horseradish, and white mustard. | allyl isothiocyanate (itc) (4) is the main pungent component in wasabi, and it generates an acrid sensation by activating trpa1. the flavor and pungency of itcs vary depending on the compound. however, the differences in activity to activate trpa1 between itcs are not known except for a few compounds. to investigate the effect of carbon chain length and substituents of itcs, the trpa1-activiting ability of 16 itcs was measured. since most of the itcs showed nearly equal trpa1-activiting potency, ... | 2015 | 26263397 |
| [immunochemical detection of azospirilla in soil with genus-specific antibodies]. | immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria azospirillum brasilense sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. cross-reactions with these proteins made it possible to use the above antibodies for detection of azospirilla as a genus-specific probe conjugated with horseradish peroxidase as an enzymatic label. direct immune-enzyme analysis of soil suspensions ( ... | 2015 | 26263631 |
| the molecular mechanism of the catalase-like activity in horseradish peroxidase. | horseradish peroxidase (hrp) is one of the most relevant peroxidase enzymes, used extensively in immunochemistry and biocatalysis applications. unlike the closely related catalase enzymes, it exhibits a low activity to disproportionate hydrogen peroxide (h2o2). the origin of this disparity remains unknown due to the lack of atomistic information on the catalase-like reaction in hrp. using qm(dft)/mm metadynamics simulations, we uncover the mechanism for reduction of the hrp compound i intermedia ... | 2015 | 26274391 |