Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| overexpression and structural characterization of the phage t4 protein dsba. | the double strand binding protein a (dsba) of bacteriophage t4 is one of several viral gene products participating in transcriptional regulation. these proteins interact or associate with the host rna polymerase core enzyme, enabling the enzyme to successively initiate transcription at different classes of viral promoters: early, middle and late. this leads to a temporally controlled expression of the t4 gene products. the dsba binding site overlaps the late promoter region, and dsba binding see ... | 1998 | 9504717 |
| identification of bacteriophage t4 virion proteins by transverse pore-gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis and dual amino acid labeling. | we have developed a horizontal n,n'-methylenebisacrylamide (bis) acrylamide gradient sodium dodecyl sulfate (sds) gel system that permits the evaluation of the purity of individual protein bands in complex mixtures. a bis gradient gel is poured vertically and, after polymerization, reoriented horizontally. a single large sample spanning the top of the gel is then run down at right angles to the gradient. the relative mobility of a few proteins varies considerably from the rest, causing them to m ... | 1997 | 9504826 |
| treatment of squamous cell carcinomas of the floor of the mouth and tongue by interstitial high-dose-rate irradiation using iridium-192. | the results are presented of afterloading high-dose-rate-radiation with iridium-192 in 34 patients with squamous cell carcinomas of the floor of the mouth and tongue. some patients were also treated surgically or given percutaneous radio- or chemotherapy. at the time of diagnosis, 28.0% had a t2 tumour, 41.9% a t3 tumour and 30.1% a t4 tumour, 41.2% had positive lymph nodes and 2.9% distant metastases. the response rate was 64.7%, 26.5% of which were complete remissions and 38.2% partial remissi ... | 1998 | 9506299 |
| quantitative eeg patterns during nose versus mouth inhalation of filtered room air in young adults with and without self-reported chemical odor intolerances. | individuals who report illness (e.g. nausea, headache) from common chemical odors tend to report cns symptoms suggestive of olfactory-limbic system involvement. this study compared the resting quantitative electroencephalographic (qeeg) patterns of young adult college students reporting subjectively elevated chemical odor intolerance ratings (hici) with those of controls reporting little or no odor intolerance (loci). each group was subdivided into those with higher (hidep) vs. lower (lodep) rat ... | 1998 | 9506309 |
| long-term changes in nodular goiter: a 5-year prospective randomized trial of levothyroxine suppressive therapy for benign cold thyroid nodules. | we studied for 5 yr a homogeneous group of 83 patients randomly assigned to a levothyroxine (l-t4) suppressive therapy or to a control group to evaluate changes in nodule or thyroid size, appearance of new nodules, and correlations with clinical parameters. in the control group, mean nodule volume increased significantly after 5 yr (2.12 +/- 1.46 vs. 1.46 +/- 0.77 ml), whereas in the treatment group it decreased, although not significantly (1.45 +/- 1.17 ml vs. 1.53 +/- 0.61 ml). baseline nodule ... | 1998 | 9506726 |
| effect of methimazole, with or without l-thyroxine, on remission rates in graves' disease. | medical treatment of graves' disease involves antithyroid drugs with or without the addition of exogenous t4. there have been conflicting reports as to whether the addition of t4 improves remission rates or delays relapse. to evaluate this issue in a north american population, 199 patients were treated with methimazole until they were euthyroid. they were then randomized to either methimazole alone in a dose sufficient to normalize tsh (group 1), or to 30 mg methimazole daily plus sufficient t4 ... | 1998 | 9506733 |
| effect of percutaneous ethanol injection therapy versus suppressive doses of l-thyroxine on benign solitary solid cold thyroid nodules: a randomized trial. | the results of studies using suppressive doses of l-t4 on benign solitary solid cold thyroid nodules have been conflicting. recently, intranodular injection of absolute ethanol has been proposed as an effective treatment, but has been evaluated only in uncontrolled studies. our objective was to evaluate the effect of two alternative medical treatment modalities, percutaneous ethanol injection therapy and l-t4, on the benign solitary solid cold thyroid nodule. in a prospective randomized clinical ... | 1998 | 9506736 |
| high serum cholesterol levels in persons with 'high-normal' tsh levels: should one extend the definition of subclinical hypothyroidism? | the association between established hypothyroidism and high cholesterol levels is well known. the aim of the present study was to investigate the effect of thyroxine (t4) administration on cholesterol levels in hypercholesterolemic subjects with tsh levels within the normal range ('high-normal' tsh compared with 'low-normal' tsh). | 1998 | 9506856 |
| anionic iodotyrosine residues are required for iodothyronine synthesis. | biosynthesis of iodothyronines in thyroglobulin occurs by oxidative coupling of two iodotyrosine residues catalyzed by thyroperoxidase. to study the mechanism of iodothyronine formation, iodine-free thyroglobulin was non-enzymatically iodinated and after removal of non-incorporated iodide, incubated with lactoperoxidase and glucose oxidase between ph 4 and 9. the amount of thyroxine (t4). 3,5,3'-tri-iodothyronine (t3), 3,3',5'-tri-iodothyronine (rt3) and 3,3'-di-iodothyronine (t2) formed was mea ... | 1998 | 9506870 |
| [interleukin-6 in patients with uveal melanoma]. | studies of the blood serum and tears of patients with uveal melanoma revealed local (14.3%) and total-systems (11.1%) production of interleukin-6 (il-6). surgery stimulates il-6 production in the serum (22.2%) and more so in tears (57.1%). the rate of il-6 detection in the serum and the iridociliary localization of the tumor correlated with tumor development from stage t3 to stage t4. however, we failed to find out precisely the role of this cytokine. | 1997 | 9508749 |
| sperm head agglutination induced by v3 peptide does not occur through a cd4 receptor. | although recent studies have shown that hiv (human immunodeficiency virus) can attach to sperm, the mechanism by which it does this is not yet understood. it has been shown that cd4 receptors on t4 cells are responsible for the binding of gp120 (glycoprotein 120) to hiv; however, the existence of cd4 receptors on sperm is controversial. the v3 peptide is part of gp120 and crucial for the syncytium formation by cd4 receptors. in this study we used an anti-cd4 antibody to block v3 peptide-induced ... | 1998 | 9509688 |
| prolactin secretion in molar and normal pregnancy. | to describe the relation between prl and hcg, t3, t4, ft4, tsh, e2 and p4 levels in normal pregnancies and those complicated with gtd. | 1998 | 9509951 |
| a randomized double-blind phase iii study of nimorazole as a hypoxic radiosensitizer of primary radiotherapy in supraglottic larynx and pharynx carcinoma. results of the danish head and neck cancer study (dahanca) protocol 5-85. | a multicenter randomized and balanced double-blind trial with the objective of assessing the efficacy and tolerance of nimorazole given as a hypoxic radiosensitizer in conjunction with primary radiotherapy of invasive carcinoma of the supraglottic larynx and pharynx. | 1998 | 9510041 |
| the ret/ptc1 rearrangement is a common feature of chernobyl-associated papillary thyroid carcinomas from belarus. | an increase in the incidence of papillary thyroid cancer has been documented in individuals exposed to chernobyl fallout in 1986. experiments using cultured human cells have suggested that radiation can induce the ret/ptc1 rearrangement involving the ret proto-oncogene. to test the hypothesis that the ret/ptc1 rearrangement is involved in the pathogenesis of chernobyl-associated papillary thyroid carcinomas, we studied a panel of 31 cases from belarus. all individuals lived in fallout-contaminat ... | 1998 | 9510121 |
| casodex (bicalutamide) 150-mg monotherapy compared with castration in patients with previously untreated nonmetastatic prostate cancer: results from two multicenter randomized trials at a median follow-up of 4 years. | to compare the efficacy, tolerability, and quality of life benefits of bicalutamide (casodex) 150-mg/day monotherapy and castration in previously untreated nonmetastatic (m0) advanced prostate cancer. | 1998 | 9510340 |
| melatonin accelerates metamorphosis in xenopus laevis. | delayed metamorphosis associated with large body size has been observed in woodhousei fowleri tadpoles reared in continuous dark (dd). to evaluate the mechanism by which dd delayed metamorphosis, light-cycle exposure was controlled and thyroxine (t4), melatonin, or drugs that alter prolactin (prl) concentrations were given to xenopus laevis tadpoles. it was hypothesized that exogenous melatonin would delay metamorphosis and increase body size, and that elevation of prl concentrations would have ... | 1998 | 9510433 |
| emergence of streptococcus pyogenes strains resistant to erythromycin in gipuzkoa, spain. | the aim of this study was to determine the evolution of resistance to macrolides and other antibiotics in strains of streptococcus pyogenes isolated in the province of gipuzkoa, spain. during the period 1984-1996, all 2561 strains of streptococcus pyogenes studied showed full susceptibility to penicillin. until 1990, only 1.2% of streptococcus pyogenes isolates were resistant to erythromycin. since then, resistance to erythromycin increased every year until 1995, when 34.8% (87/250) of streptoco ... | 1998 | 9512178 |
| hyperthyroidism: an unusual case presentation. | hyperthyroidism is the most common disorder of the thyroid. patients typically present with complaints consistent with a hypermetabolic state, including nervousness, weight loss, heat intolerance, palpitations, irritability, and tremor. this case report reviews a 34-year-old woman who presented with unilateral upper extremity weakness, weight gain, and an episode of atrial fibrillation, the latter coinciding with a 36-hour lack of sleep and excess alcohol and caffeine intake. although an extensi ... | 1998 | 9513218 |
| toxicity study of a rubber antioxidant, 2-mercaptobenzimidazole, by repeated oral administration to rats. | the chemical structure of 2-mercaptobenzimidazole (2-mbi), which is widely used as a rubber antioxidant, is partially similar to those of thiourea (tu) and ethylenethiourea (etu), both potent thyrotoxic compounds. in order to determine the oral toxicity of 2-mbi, a 28-day repeated dose toxicity study in wistar rats followed by observation over a 14-day recovery period was conducted at dose levels of 2, 10 and 50 mg/kg 2-mbi administered by gavage. no toxic deaths occurred due to 2-mbi treatment. ... | 1998 | 9513921 |
| nonsteroidal antiinflammatory drug-photosensitized formation of pyrimidine dimer in dna. | phototoxic nonsteroidal antiinflammatory drugs (nsaids) may induce dna damage in vitro upon irradiation. in this study, we investigated the ability of ketoprofen (kp), tiaprofenic acid (tia), naproxen (np) and indomethacin (ind) to photosensitize the formation of pyrimidine dimers and single strand breaks. both kinds of damage were sought by analyzing dna-drug mixtures irradiated at 313 nm by agarose gel electrophoresis. the formation of pyrimidine dimers was evidenced by using endonuclease v fr ... | 1998 | 9514078 |
| enhancement of gap junctional communication and connexin43 expression by thyroid hormones. | cells in tissues coordinate their activity by sharing ions, second messengers, and small metabolites through clusters of intercellular channels called gap junctions. the thyroid hormones 3,3',5-triiodo-l-thyronine (t3) and l-thyroxine (t4) are capable of modulating gap junctional communication (gjc) as are 1,25-dihydroxyvitamin d3, retinoic acid, and other nuclear receptor ligands. t3 and t4 were found to stimulate gjc in wb-f344 rat liver epithelial cells dose-dependently at concentrations betw ... | 1998 | 9514082 |
| studies on inhibitors of mammalian dna polymerase alpha and beta: sulfolipids from a pteridophyte, athyrium niponicum. | three sulfolipid compounds, 1, 2, and 3, have been isolated from a higher plant, a pteridophyte, athyrium niponicum, as potent inhibitors of the activities of calf dna polymerase alpha and rat dna polymerase beta. the inhibition by the sulfolipids was concentration dependent, and almost complete inhibition of dna polymerase alpha and dna polymerase beta was achieved at 6 and 8 microg/ml, respectively. the compounds did not influence the activities of calf thymus terminal deoxynucleotidyl transfe ... | 1998 | 9514090 |
| subdomain interactions as a determinant in the folding and stability of t4 lysozyme. | the folding of large, multidomain proteins involves the hierarchical assembly of individual domains. it remains unclear whether the stability and folding of small, single-domain proteins occurs through a comparable assembly of small, autonomous folding units. we have investigated the relationship between two subdomains of the protein t4 lysozyme. thermodynamically, t4 lysozyme behaves as a cooperative unit and the unfolding transition fits a two-state model. the structure of the protein, however ... | 1998 | 9514264 |
| the response of t4 lysozyme to large-to-small substitutions within the core and its relation to the hydrophobic effect. | to further examine the structural and thermodynamic basis of hydrophobic stabilization in proteins, all of the bulky non-polar residues that are buried or largely buried within the core of t4 lysozyme were substituted with alanine. in 25 cases, including eight reported previously, it was possible to determine the crystal structures of the variants. the structures of four variants with double substitutions were also determined. in the majority of cases the "large-to-small" substitutions lead to i ... | 1998 | 9514271 |
| blood selenium and glutathione peroxidase status in patients with colorectal cancer. | it is still controversial whether a low selenium level and a reduced activity of the selenium-dependent enzyme, glutathione peroxidase, in blood are associated with an increased risk and poor prognosis of cancer in humans. this study evaluates whether colorectal cancer patients have lower serum selenium and glutathione peroxidase levels than a gender-matched and age-matched control group and whether there is a correlation to clinical data and prognosis. | 1998 | 9514428 |
| crystal structure of the lysozyme from bacteriophage lambda and its relationship with v and c-type lysozymes. | like other lysozymes, the bacteriophage lambda lysozyme is involved in the digestion of bacterial walls. this enzyme is remarkable in that its mechanism of action is different from the classical lysozyme's mechanism. from the point of view of protein evolution, it shows features of lysozymes from different classes. the crystal structure of the enzyme in which all tryptophan residues have been replaced by aza-tryptophan has been solved by x-ray crystallography at 2.3 a using a combination of mult ... | 1998 | 9514719 |
| generation of ligand binding sites in t4 lysozyme by deficiency-creating substitutions. | several variants of t4 lysozyme have been identified that sequester small organic ligands in cavities or clefts. to evaluate potential binding sites for non-polar molecules, we screened a number of hydrophobic large-to-small mutants for stabilization in the presence of benzene. in addition to leu99-->ala, binding was indicated for at least five other mutants. variants met102-->ala and leu133-->gly, and a crevice mutant, phe104-->ala, were further characterized using x-ray crystallography and the ... | 1998 | 9514755 |
| a vibriophage, kvp40, with major capsid protein homologous to gp23* of coliphage t4. | the mcp gene encoding the major capsid protein (mcp) of vibriophage kvp40, a large-tailed dna phage, was cloned and sequenced. the nucleotide sequence of the mcp gene was 64.4% similar to that of gene 23 of coliphage t4. analysis of the n-terminal amino acid sequence of purified native mcp revealed that the mcp gene actually coded for a precursor, pro-mcp, whose 62 n-terminal amino acids must be removed upon maturation to mcp. thus, mature mcp would consist of 452 amino acid residues and have a ... | 1998 | 9514973 |
| extended surgical resection in t4 gastric cancer. | some physicians still consider invasion of adjacent organs by the carcinoma of stomach as a sign of incurable disease. | 1998 | 9515528 |
| endonuclease ii of coliphage t4: a recombinase disguised as a restriction endonuclease? | endoii shares with restriction endonucleases the property of cleaving foreign dna while leaving the endonuclease-encoding genome intact, ensuring the survival of one dna species in the cell. in addition, in vivo endoii cleaves a specific dna sequence and cleavage is context dependent. these context effects extend over at least 1000 bp, largely limiting cleavage to once within this distance. like homing endonucleases, in vivo endoii recognizes a long, asymmetric and degenerate consensus sequence ... | 1998 | 9515694 |
| the bacteriophage t4 asia protein: a molecular switch for sigma 70-dependent promoters. | the asia protein, encoded by bacteriophage t4, inhibits esigma70-dependent transcription at bacterial and early-phage promoters. we demonstrate that the inhibitory action of asia involves interference with the recognition of the -35 consensus promoter sequence by host rna polymerase. in vitro experiments were performed with a c-terminally labelled sigma factor that is competent for functional holoenzyme reconstitution. by protease and hydroxyl radical protein footprinting, we show that asia bind ... | 1998 | 9515707 |
| mutations of the bacteriophage t4 type ii dna topoisomerase that alter sensitivity to antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide and an antibacterial quinolone. | various antitumor and antibacterial agents target type ii dna topoisomerases, stabilizing a cleaved dna reaction intermediate and thereby converting topoisomerase into a cellular poison. two 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-amsa)-resistant bacteriophage t4 topoisomerases have previously been characterized biochemically, and we have now determined the sequence of the causative mutations. in one case, a mutation (e457k) in a conserved domain of gp39 (atpase subunit) causes resista ... | 1998 | 9515814 |
| radiation therapy after prostatectomy: now or later? | patients with pathological stage t3 or t4 prostate cancers who have undetectable prostate-specific antigen (psa) levels after radical retropubic prostatectomy (rrp) have a substantial risk of recurrence. the first sign of recurrence is a rising psa level, which precedes clinical evidence of failure by months to years. adjuvant radiation therapy can significantly reduce the risk of failure in these patients. patients with rising psa levels after rrp can be salvaged with radiation therapy. larger ... | 1998 | 9516594 |
| essential dynamics of dna containing a cis.syn cyclobutane thymine dimer lesion. | conformational properties of a uv-damaged dna decamer containing a cis.syn cyclobutane thymine dimer (pd) have been investigated by molecular dynamics (md) simulations. results from md simulations of the damaged decamer dna show a kink of approximately 21.7 degrees at the pd damaged site and a disruption of h bonding between the 5'-thymine of the pd and its complementary adenine. however, no extra-helical flipping of the 3'-adenine complementary to the pd was observed. comparison to two undamage ... | 1998 | 9518486 |
| [the staging of renal-cell carcinomas in mrt and ct--a prospective histologically controlled study]. | to evaluate the accuracy of computed tomography (ct) and magnetic resonance imaging (mri) in staging renal carcinoma. | 1998 | 9519049 |
| the influence of smoking habits on thyroid gland volume: an ultrasonic approach. | the effects of smoking habits on thyroid function, echo-texture (nodules and/or cysts) and thyroid gland volume were determined by using ultrasound and measuring serum thyroxin (t4), triiodothyronine (t3), thyrotropin (tsh) and tpo antibodies (ab-tpo) in 189 healthy smokers and non-smokers, randomly selected (111 females and 78 males) among the employees of our hospital and their relatives. when the entire group of subjects was considered the mean ratio of thyroid gland volume/body weight was fo ... | 1997 | 9519672 |
| the pnk/pnl gene (orf 86) of autographa californica nucleopolyhedrovirus is a non-essential, immediate early gene. | autographa californica nucleopolyhedrovirus (acmnpv) orf 86, located within the hindiii c fragment, potentially encodes a protein which shares sequence similarity with two t4 bacteriophage gene products, rna ligase and polynucleotide kinase. this acmnpv gene has been designated pnk/pnl but has yet to be assigned a function in virus replication. it has been classified as an immediate early virus gene, since the promoter was active in uninfected insect cells and mrna transcripts were detectable fr ... | 1998 | 9519844 |
| bacterial load and protein deposits on 15-day versus 1-day disposable hydrophilic contact lenses. | this study quantified the bacterial load and protein deposits on 1- and 15-day disposable contact lenses after use in normal wearers. | 1998 | 9520189 |
| identification of a transient excision intermediate at the crossroads between dna polymerase extension and proofreading pathways. | dna polymerases achieve accurate dna replication through a delicate balance between primer elongation and proofreading. while insufficient proofreading results in dna replication errors, indiscriminate removal of correct along with incorrect nucleotides is wasteful and may prevent completion of dna synthesis. the transition between polymerization and proofreading modes is proposed to be governed by a kinetic barrier that prevents proofreading unless the rate of primer elongation is significantly ... | 1998 | 9520396 |
| [thyroid hormone resistance. clinical, biochemical and genetic study of a family]. | we here present a family where three individuals in three generations had varying degrees of goiter, tachycardia, fatigue, hyperactivity, and learning disability. serum t3 and free t4 were elevated, whereas tsh was normal or slightly increased. the clinical findings in combination with the hormone values led to several supplementary investigations and therapies being carried out, but they had no beneficial influence on the patients' symptoms. the commonest form of thyroid hormone resistance (rth ... | 1998 | 9520578 |
| cyclobutane thymine dimers with a disrupted phosphodiester bond are refractory to t4 endonuclease v digestion but have increased sensitivity to uvrabc nuclease. | uv irradiation induces the dimerization of synthetic single-stranded, 80-mer oligonucleotides with self-complementary, alternating purine-pyrimidine sequences, and terminal 5'- and 3'-thymines; this process can be reversed by photoreactivation. the uv-induced 160-mers are sensitive to digestion by the restriction enzyme snabi, but monomers are insensitive to digestion, indicating that uv irradiation stabilizes the formation of double-stranded dna. these results suggest that uv irradiation of the ... | 1998 | 9521643 |
| an intercalated and thermally stable fapy adduct of aflatoxin b1 in a dna duplex: structural refinement from 1h nmr. | the structure of a formamidopyrimidine (fapy) adduct arising from imidazole ring opening of the initially formed trans-8, 9-dihydro-8-(n7-guanyl)-9-hydroxyaflatoxin b1 adduct under basic conditions and positioned in the 5'-d(ctatfapygattca)-3'*5'-d(tgaatcatag)-3' oligodeoxynucleotide was determined. the fapy adduct may be a major progenitor of aflatoxin b1-induced mutations in dna. the freshly prepared sample showed biphasic melting, with transitions at 28 and 56 degreesc. nmr initially showed m ... | 1998 | 9521757 |
| suppressive balf lymphocyte phenotype in a patient with prolonged stable alveolar proteinosis. | the case history of a 25-year-old man suffering from idiopathic alveolar proteinosis is described. the patient showed a prolonged stable mild disease and a distinct suppressive phenotypic profile of balf lymphocytes. specifically, a low t4/t8 ratio and a high percentage of cd11b+ t8 lymphocytes was found. correlations with disease expression are made. | 1998 | 9523372 |
| morphometric x-ray absorptiometry: reference data for vertebral dimensions. | vertebral fractures are a common and important consequence of osteoporosis and are often identified via morphometric analysis of conventional lateral spine radiographs (morphometric radiography or mrx). a new method of performing vertebral morphometry using images acquired on dual-energy x-ray absorptiometry (dxa) scanners (morphometric x-ray absorptiometry or mxa) has recently been developed. in this study, we derive reference data for vertebral heights and height ratios using mxa scans as the ... | 1998 | 9525347 |
| bombyx mori nucleopolyhedrovirus encodes a dna-binding protein capable of destabilizing duplex dna. | a dna-binding protein (designated dbp) with an apparent molecular mass of 38 kda was purified to homogeneity from bmn cells (derived from bombyx mori) infected with the b. mori nucleopolyhedrovirus (bmnpv). six peptides obtained after digestion of the isolated protein with achromobacter protease i were partially or completely sequenced. the determined amino acid sequences indicated that dbp was encoded by an open reading frame (orf16) located at nucleotides (nt) 16189 to 17139 in the bmnpv genom ... | 1998 | 9525636 |
| the action of dna ligase at abasic sites in dna. | apurinic/apyrimidinic (ap) sites occur frequently in dna as a result of spontaneous base loss or following removal of a damaged base by a dna glycosylase. the action of many ap endonuclease enzymes at abasic sites in dna leaves a 5'-deoxyribose phosphate (drp) residue that must be removed during the base excision repair process. this 5'-drp group may be removed by ap lyase enzymes that employ a beta-elimination mechanism. this beta-elimination reaction typically involves a transient schiff base ... | 1998 | 9525883 |
| random mutagenesis by whole-plasmid pcr amplification. | random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular dna sequence. as a prime example, libraries of randomized cdna clones, when translated into their corresponding proteins, can be useful in investigating the functional contributions of a mutagenized region to the overall properties of a protein. existing molecular cloning techniques for random mutagenesis are tedious and frequently plagued with high levels of background from w ... | 1998 | 9526653 |
| the effect of dehydroepiandrosterone combined with a low-fat diet in spontaneously obese dogs: a clinical trial. | dehydroepiandrosterone (dhea) has been shown to have antiobesity activity in rodents and spontaneously obese dogs. this study evaluated the effect of dhea or placebo combined with a low-fat/high-fiber diet in spontaneously obese dogs in a clinical trial. spontaneously obese, euthyroid dogs, referred to the university of wisconsin school of veterinary medicine for treatment of their obesity, were evaluated for percent overweight, rate of weight loss, serum cholesterol, plasma lipoprotein and seru ... | 1998 | 9526966 |
| preoperative hyperthermia combined with radiochemotherapy in locally advanced rectal cancer: a phase ii clinical trial. | a prospective phase ii study was performed to determine the feasibility and efficacy in terms of response rate, resectability, and morbidity in patients with locally advanced rectal cancer who received preoperative regional hyperthermia combined with radiochemotherapy (hrct). | 1998 | 9527061 |
| esophageal carcinoma: depth of tumor invasion is predictive of regional lymph node status. | the depth of tumor invasion (t) and regional lymph node status (n) are two factors that define the stage of an esophageal carcinoma. however, the arrangement of staging groups assumes that these factors are independent variables. a retrospective review of 359 consecutive patients undergoing esophageal resection was conducted to define the relationship between t and n and to determine whether t is a significant predictor of regional lymph node metastasis (n1). | 1998 | 9527214 |
| genetic analysis of the ul 15 gene locus for the putative terminase of herpes simplex virus type 1. | the herpes simplex virus (hsv-1) ul15 gene encodes one of the six viral gene products required for viral dna cleavage and packaging. ul15 is a spliced gene and encodes two separately translated proteins, ul15 and ul15.5. sequence analysis reveals that ul15 shares homology with gp 17, the large catalytic subunit of the bacteriophage t4 terminase, a protein which cleaves the polymeric t4 dna into monomers. both proteins contain a putative atp binding motif known as the walker a and b boxes. in thi ... | 1998 | 9527913 |
| physiological effects of some synthetic food colouring additives on rats. | three different synthetic chocolate colourant agents (a, b and c) were administered to healthy adult male albino rats for 30 and 60 day periods to evaluate their effects on body weight, blood picture, liver and kidney functions, blood glucose, serum and liver lipids, liver nucleic acids (dna and rna), thyroid hormones (t3 and t4) and growth hormone. in addition, histopathological examinations of liver, kidney and stomach sections were studied. these parameters were also investigated 30 days afte ... | 1997 | 9528169 |
| early coronary angiogenesis in response to thyroxine: growth characteristics and upregulation of basic fibroblast growth factor. | although a substantial coronary angiogenesis occurs after thyroid hormone treatment, its regulation and relationship to cardiac hypertrophy are not understood. this study was designed to determine (1) the onset of capillary proliferation, (2) the sites of capillary proliferation, and (3) whether basic fibroblast growth factor (bfgf) upregulation occurs in response to thyroxine administration. male sprague-dawley rats were injected daily with l-thyroxine (t4, 0.2 mg/kg s.c.). bromodeoxyuridine la ... | 1998 | 9529163 |
| the early phase of epidermal barrier regeneration is faster in patients with atopic eczema. | altered epidermal barrier function as determined by transepidermal water loss (tewl) is a typical feature in patients with atopic eczema (ae). | 1997 | 9529552 |
| compensation of decreased triose phosphate/phosphate translocator activity by accelerated starch turnover and glucose transport in transgenic tobacco. | tobacco (nicotiana tabacum l.) plants were transformed with an antisense construct of the chloroplast triose phosphate/phosphate translocator (tpt). three transformant lines of the t4 progeny, which showed a large decrease in the transcript level of the tpt were used for further biochemical and physiological characterisation. in all antisense lines tested, tpt transport activity was diminished by 50-70% compared with the wild type (wt). despite this high reduction in the transport capacity, alph ... | 1998 | 9530880 |
| free plasma catecholamines in spinal cord injured persons with different injury levels at rest and during exercise. | spinal cord lesion leads to an interruption of pathways from brain to the peripheral sympathetic nervous system, which results in pathological changes in sympathetic innervation. free epinephrine (e), norepinephrine (ne) and dopamine (da) were measured in 30 tetraplegics (tetra), 15 high-lesion paraplegics (t1 and t4, hpara), 15 paraplegics with injuries between t5 and t10 (mpara), 15 low-lesion paraplegics (below t10, lpara) and 16 non-handicapped control persons (c) at rest, at 60 and 100% of ... | 1998 | 9531449 |
| in ovo treatment with an aromatase inhibitor masculinizes postnatal hormone levels, abdominal fat pad content, and gh pulsatility in broiler chickens. | vorozole, a selective aromatase inhibitor, was administered in ovo to test the specific embryonic role of estrogen in conferring the sex distinction in gh release and body phenotype in broilers. on day 6 of incubation, eggs were injected with saline or with different concentrations of vorozole. postnatal blood samples were analyzed for t3, t4, gh, estradiol (e2), and testosterone (t). at the age of 4 wk, control and vorozole-treated birds were cannulated, and serial blood samples were withdrawn ... | 1998 | 9532425 |
| [hypothalamo-pituitary-thyroid system in patients with rheumatoid arthritis]. | study of the function of the hypothalamus-pituitary-thyroid system in rheumatoid arthritis (ra) materials and methods: three hundred and fifty patients with ra of different forms, stages, and intensity were examined. the blood and synovial fluid (sf) levels of thyroid-stimulating hormone (tsh), thyroxine (t4) and triiodothyronine (t3) were measured by immunoradiometric assay. | 1998 | 9532653 |
| [localized and locally advanced renal cell carcinoma: method of outcome prognosis]. | basing on the long-term results of nephrectomy in 180 patients with renal cell carcinoma, the detailed assessment has been made of implications of a number of factors in 5-year survival and contribution of these factors to the disease outcome. the author proposes a method of prognosticating the disease outcome in patients with localized and locally advanced renal carcinoma which enables specification of the existing international classification and more precise prognosis of the disease. at stage ... | 1998 | 9532944 |
| effect of the yang tonifying herbs on myocardial beta-adrenoceptors of hypothyroid rabbits. | new zealand white female rabbits were randomly divided into three groups, each contained six rabbits, i.e. thyroidectomized and untreated rabbits (group 1), thyroidectomized rabbits treated by the yang tonifying herbs (group 2) and sham thyroidectomized rabbits as controls (group 3). the myocardial beta-adrenoceptor density (bmax) and affinity (kd) of each group of rabbits were determined by radioligand binding assay technique on the thirtieth postoperative day and the data were handled by using ... | 1998 | 9533431 |
| inhibition of cell growth and telomerase activity of breast cancer cells in vitro by 3'-azido-3'-deoxythymidine. | the effect of zidovudine (3'-azido-3'-deoxythymidine; azt) was investigated in four breast cancer cell lines, a t4 cell leukemia, and a normal breast cell line in vitro. azt inhibited the growth of all tumoral cell lines, but it did so in a wide range of concentrations. the growth of a normal breast cell line was also inhibited, although it required a much higher concentration. furthermore, azt inhibited colony formation in soft agar and telomerase activity. these results indicated that azt can ... | 1998 | 9533539 |
| epitope mapping of t4 endonuclease vii with monoclonal antibodies reveals importance of both ends of the protein for target binding. | endonuclease vii (endo vii) of bacteriophage t4 is a holliday-structure resolving enzyme that can also recognize many other defects in dna via an altered secondary structure. the protein has a molecular mass of 18 kda and exists as a dimer in solution. here we report the production and characterization of monoclonal antibodies (mabs) directed against the highly purified enzyme. from one fusion 15 hybrid cell lines producing mabs with high affinity for endo vii could be established. the mabs were ... | 1998 | 9533878 |
| the largest (70 kda) product of the bacteriophage t4 dna terminase gene 17 binds to single-stranded dna segments and digests them towards junctions with double-stranded dna. | bacteriophage terminases are oligomeric multifunctional proteins that bind to vegetative dna, cut it and, together with portal proteins, translocate the dna into preformed heads. most terminases are encoded by two partially overlapping genes. in phage t4 they are genes 16 and 17. we have shown before that the larger of these, gene 17, can yield, in addition to a full-length 70 kda product, several shorter peptides. at least two of these, gene product (gp) 17' and gp17", are initiated in the same ... | 1998 | 9533879 |
| efficacy and safety of iodine in the postpartum period in an area of mild iodine deficiency. | iodine deficiency (even moderate) plays a major role in pregnancy associated goiter development, which is only party reversible after pregnancy. the prevalence of post partum thyroiditis is reported to be slightly lower in areas of iodine deficiency. thus iodine supplementation may be effective in decreasing pregnancy associated increase in thyroid volume, but enhances the risk of increasing the prevalence of thyroid dysfunction in the post partum period. therefore, we evaluated the effect of io ... | 1998 | 9533929 |
| central hyperthyroidism. | central hyperthyroidism is a rare condition in which thyrotoxicosis results from primary overproduction of tsh by the pituitary gland with subsequent thyroid enlargement and hyperfunction. the two known causes of central hyperthyroidism are tsh-producing pituitary tumors (tshomas) and the syndrome of prth. both of these entities are characterized by clinical thyrotoxicosis, diffuse goiters, elevated circulating levels of free t4 and t3, and a nonsuppressed serum tsh. it is critical to distinguis ... | 1998 | 9534036 |
| treatment of hyperthyroidism with radioactive iodine. | treatment of hyperthyroidism with rai has been performed for more than a half century with efficacy and safety. for its optimal use, the physician must employ appropriate patient selection criteria and clinical judgment concerning pretreatment patient preparation. the dose of the 131i needed remains an area of uncertainty and debate; thus far, it has not been possible to resolve the trade-off between efficient definitive cure of hyperthyroidism and the high incidence of post-therapy hypothyroidi ... | 1998 | 9534037 |
| influence of hypo- or hyperthyroidism on ovarian function in brahman cows. | multiparous brahman cows (n = 21) were randomly assigned during late fall within bw and body condition score (bcs) to receive either 3.0 ml of corn oil (c; n = 7), 3.0 mg/(cow x d) triiodothyronine (t3) s.c. in 3.0 ml of corn oil (hyper; n = 7), or 4.0 mg/(kg x d) 6-n-propyl-2-thiouracil (ptu; fed with concentrate) plus 3.0 ml/d corn oil (hypo; n = 7). water, minerals, and coastal bermudagrass hay were available free choice, and all cows received 3.2 kg x cow(-1) x d(-1) of 5:1 corn:soybean meal ... | 1998 | 9535350 |
| selective pituitary resistance to thyroid hormone produced by expression of a mutant thyroid hormone receptor beta gene in the pituitary gland of transgenic mice. | resistance to thyroid hormone (rth) has been subdivided into generalized resistance (grth) and pituitary resistance (prth) based on the clinical impression of absence or presence of thyrotoxicosis. however, due to lack of objective clinical and genetic criteria, the existence of prth as a distinct entity became controversial. to determine what the phenotype would be if rth was confined to the pituitary, a transgenic mouse was developed in which expression of the mutant thyroid hormone receptor ( ... | 1998 | 9535809 |
| effect of indian red scorpion (mesobuthus tamulus concanesis, pocock) venom on thyroxine and triiodothyronine in experimental acute myocarditis and its reversal by species specific antivenom. | acute myocarditis was induced in dogs and rabbits by injection of scorpion (m. tamulus concanesis, earlier called buthus tamulus) venom by s.c., i.m. or i.v. routes. a decrease in thyroxine (t4) levels was observed following i.v. injection of venom in dogs. in rabbits the venom (i.m.) did not elicit any change in t4 levels. envenomation (s.c.) resulted in a reduction in triiodothyronine (t3) levels in dogs. venom injection (s.c.) along with i.v. administration of the species specific antivenom ( ... | 1998 | 9536646 |
| efficiency of t4 gene 60 translational bypassing. | ribosomes translating bacteriophage t4 gene 60 mrna bypass 50 noncoding nucleotides from a takeoff site at codon 46 to a landing site just upstream of codon 47. a key signal for efficient bypassing is contained within the nascent peptide synthesized prior to takeoff. here we show that this signal is insensitive to the addition of coding information at its n terminus. in addition, analysis of amino-terminal fusions, which allow detection of all major products synthesized from the gene 60 mrna, sh ... | 1998 | 9537381 |
| nutritional and prognostic significance of serum hypothyroxinemia in hospitalized patients with liver cirrhosis. | a variety of severe illnesses can induce changes in thyroid hormone metabolism, leading to findings referred to as "sick euthyroid syndrome". in several groups of patients the reduction of serum thyroxine concentration (t4), characteristic of the low-t4 variant of sick euthyroid syndrome, has been found to be a good predictor of survival. although the pathophysiology of hormonal alterations has not yet been defined, nutritional deficits have been suggested to play a role. the study aimed to defi ... | 1998 | 9537848 |
| the mota transcriptional activator of bacteriophage t4 binds to its specific dna site as a monomer. | during bacteriophage t4 middle mode gene expression, the mota transcription factor binds to t4 middle promoters at a -30 mot box consensus sequence to allow activation. previous binding studies showed that mota forms multiple gel-shifted complexes with dna, and structural evidence suggested that mota dimerizes upon dna binding. we have shown that a short (13 bp) mot box dna substrate binds mota protein but fails to form slower migrating complexes. therefore, the slower migrating complexes in gel ... | 1998 | 9538016 |
| amiodarone and the thyroid: a practical guide to the management of thyroid dysfunction induced by amiodarone therapy. | amiodarone induces predictable changes in thyroid function tests that are largely explicable in terms of the physiological effects of iodide excess and inhibition of deiodinase activity. clinically relevant thyroid dysfunction is not uncommon during amiodarone therapy, and requires careful diagnosis and treatment. the diagnosis and management of thyrotoxicosis is probably best supervised by a specialist endocrinologist. control of hypothyroidism can generally be achieved simply by the addition o ... | 1998 | 9538302 |
| effect of 17 beta-estradiol, retinoic acid and tamoxifen upon primary and transplanted thyroid tumor in b6c3f1 mice fed an iodine deficient diet. | this study was aimed to establish tsh dependent, transplantable thyroid tumor (tt) in b6c3f1 (bcf1) mice. in addition, transplanted tt was examined for its growth in mice given 17 beta-estradiol (e2), retinoic acid (ra), tamoxifen (tam), t3 and t4. both sexes of bcf1 mice were observed for 12 months under idd and distilled water (dw), starting at 4 weeks of age. groups of mice received an i.p. injection of radioactive iodine (131i) once at a dose of 60 mu ci/head and/or given 0.25 mg e2 pellet s ... | 1997 | 9538564 |
| late components of the event-related potentials and their topography in parkinson's disease. | late components of the event-related potential (erp; n100, p200, n200, and p300) were elicited using an auditory oddball paradigm (with a button-press response to target stimuli) in 15 parkinson's disease (pd) patients and 50 normal control subjects. compared with control subjects, pd subjects showed a significant decrease in n200 amplitude. between-group topographical differences in n200 amplitude were evident at central (c3, cz, c4) and temporal (t5, t3, t4, t6) regions. the results may reflec ... | 1998 | 9539339 |
| neoadjuvant concurrent chemoradiotherapy followed by definitive high-dose radiotherapy or surgery for operable thoracic esophageal carcinoma. | a prospective clinical trial was undertaken to investigate the feasibility of concurrent chemoradiotherapy for esophageal carcinomas. | 1998 | 9539559 |
| context-dependent protein stabilization by methionine-to-leucine substitution shown in t4 lysozyme. | the substitution of methionines with leucines within the interior of a protein is expected to increase stability both because of a more favorable solvent transfer term as well as the reduced entropic cost of holding a leucine side chain in a defined position. together, these two terms are expected to contribute about 1.4 kcal/mol to protein stability for each met --> leu substitution when fully buried. at the same time, this expected beneficial effect may be offset by steric factors due to diffe ... | 1998 | 9541409 |
| autoimmune thyroid disease and insulin-dependent diabetes mellitus during pregnancy and post partum. | the aim of this study was to assess thyroid dysfunction and autoimmunity in pregnant insulin-dependent diabetes mellitus (iddm) women during pregnancy and early post partum. fifteen pregnant iddm women and 77 healthy pregnant women were studied. free t4, tsh, tpo-ab and tg-ab were assayed during the first and third trimester of pregnancy and 3 months post partum. in iddm women ft4 levels significantly decreased (p < 0.05) during third trimester and 3 months post partum and also tpo-ab during thi ... | 1997 | 9542278 |
| long-term action of potassium bromide on the rat thyroid gland. | male rats fed by a standard diet with determined of bromine and iodine content were exposed to a 133-day oral administration of kbr (100, 200, 400 mg br-/l drinking water). their thyroid glands showed increased growth of the epithelial cells reflected by a microfollicular rearrangement of the parenchyma due to proliferation of very small follicles with a low or zero content of colloid. morphometric analysis of thyroids of br(-)-exposed animals revealed a significant decrease in the volume of int ... | 1998 | 9542578 |
| possible role of gh/igf-1 in the ovarian function of adult hypothyroid rats. | we have monitored estrous cycle and measured serum estradiol, gh, igf-1, t4 and t3 levels in adult hypothyroid female rats which were divided into four groups: h group, hypothyroid rats without treatment; h-t4 group, hypothyroid rats injected daily with t4; ht4-ptu group, hypothyroid rats injected daily with t4 plus ptu (propylthiouracil), and h-t4-iop group, hypothyroid rats injected daily with t4 plus iop (iopanoic acid); euthyroid rats (e group) were used as control. our results indicate that ... | 1998 | 9543343 |
| effects of subchronic exposure to cadmium chloride on endocrine and metabolic functions in rainbow trout oncorhynchus mykiss. | adult and juvenile rainbow trout, oncorhynchus mykiss, were exposed for 30 days to cdcl2 in water (10 and 25 micrograms cd/l for adults, and 1 and 5 micrograms cd/l for juveniles) to investigate effects of subchronic exposures to cd on the hypothalamo-pituitary-interrenal and the hypothalamo-pituitary-thyroid axis, and on metabolic status. liver size and glycogen content and body mass gain were significantly reduced in the exposed adult fish and similar changes were detected in the juveniles. pl ... | 1998 | 9543508 |
| induction of oral tolerance in human autoimmune thyroid disease. | our laboratory has reported suppression of experimental autoimmune thyroiditis in mice by oral feeding with antigen. based on these data, we considered it possible that oral feeding of animal thyroglobulin (tg) might induce tolerance to antigen in human autoimmune thyroid disease (aitd). thirteen patients receiving thyroid hormone replacement with synthetic thyroxine (t4) (five patients with graves' disease, treated with radioiodine 4 to 11 years ago and eight patients with hashimoto's thyroidit ... | 1998 | 9545109 |
| simultaneous measurement of free thyroxine and free 3,5,3'-triiodothyronine in undiluted serum by direct equilibrium dialysis/radioimmunoassay: evidence that free triiodothyronine and free thyroxine are normal in many patients with the low triiodothyronine syndrome. | we have devised a practical, sensitive and specific method for simultaneous measurement of free thyroxine (ft4) and free triiodothyronine (ft3) in undiluted serum by direct equilibrium dialysis radioimmunoassay (ria). two hundred microliters serum sample was dialyzed against buffer (ph 7.4) for 20 hours at 37 degrees c and approximately 800 microl of the dialysate was used for measuring ft4 and ft3 simultaneously. the assay was set up in polystyrene tubes coated with anti-t4 antibody and availab ... | 1998 | 9545112 |
| visualizing metal-ion-binding sites in group i introns by iron(ii)-mediated fenton reactions. | most catalytic rnas depend on divalent metal ions for folding and catalysis. a thorough structure-function analysis of catalytic rna therefore requires the identification of the metal-ion-binding sites. here, we probed the binding sites using fenton chemistry, which makes use of the ability of fe2+ to functionally or structurally replace mg2+ at ion-binding sites and to generate short-lived and highly reactive hydroxyl radicals that can cleave nucleic acid and protein backbones in spatial proxim ... | 1998 | 9545425 |
| granule associated dnase in t4 and t8 lymphocytes from patients with autoimmune diseases. | the presence of a dnase activity associated with secretion granules was detected in t4 and t8 lymphocytes from patients with autoimmune diseases. this activity was much higher in primary biliary cirrhosis (pbc) than in graves' disease (gd) and multiple sclerosis (ms) or in healthy subjects. this granule associated dnase activity was ca(2+)-dependent, inhibited by zn2+, and higher at low ph; its molecular weight corresponded to 66kda; it was more active with double-strand than single-strand dna. ... | 1998 | 9545531 |
| pharmacological and toxicological effects of chronic porcine growth hormone administration in dogs. | the purpose of this study was to evaluate the pharmacological and toxicological effects of exogenous gh administration in normal adult dogs. because porcine gh (pgh) is structurally identical to canine gh, pgh was selected for a 14-wk study in dogs. thirty-two dogs (< 2 yr) were randomized to 4 groups (4 dogs/sex/group); 1 group was treated with the vehicle and 3 groups received pgh at 0.025, 0.1, or 1.0 iu/kg/day subcutaneously. daily clinical signs and weekly body weights were recorded. hemato ... | 1998 | 9547855 |
| the uvsy recombination protein of bacteriophage t4 forms hexamers in the presence and absence of single-stranded dna. | a prerequisite to genetic recombination in the t4 bacteriophage is the formation of the presynaptic filament-a helical nucleoprotein filament containing stoichiometric amounts of the uvsx recombinase in complex with single-stranded dna (ssdna). once formed, the filament is competent to catalyze homologous pairing and dna strand exchange reactions. an important component in the formation of the presynaptic filament is the uvsy protein, which is required for optimal uvsx-ssdna assembly in vitro, a ... | 1998 | 9548953 |
| reciprocal angulation of vertebral bodies in the sagittal plane in an asymptomatic greek population. | a prospective study conducted on several roentgenographic parameters of the standing sagittal profile of the spine in an asymptomatic greek population. | 1998 | 9549792 |
| paraparesis associated with mild congenital kyphoscoliosis in an adult. | a 48-year-old man suffering from paraparesis had congenital kyphoscoliosis due to fused wedged vertebrae between t2 and t4. the kyphoscoliosis consisted of left convex scoliosis measuring 26 degrees and kyphosis measuring 27 degrees. on ct-myelograms and axial mr images, the dura and spinal cord were deviated anterolaterally to the concave side of the curve around its apex. the spinal cord was stuck and flattened against the posterolateral margin of the vertebral body to the base of the pedicle ... | 1997 | 9549831 |
| effects of thyroid hormone (thyroxine) and testosterone on hepatic 11beta-hydroxysteroid dehydrogenase mrna and activity in pubertal hypothyroid male rats. | to investigate the effects of thyroid hormone and testosterone on 11beta-hydroxysteroid dehydrogenase type 1 (11beta-hsd1), we measured changes in hepatic 11beta-dehydrogenase activity and its mrna levels in pubertal methimazole (mmi)-induced hypothyroid male rats following treatment with thyroxine ([t4] 50 microg/kg/d) or testosterone (250 microg/d) for 14 days. hypothyroidism in male rats markedly reduced hepatic 11beta-hsd1 mrna levels and serum testosterone concentrations (p < .01). subcutan ... | 1998 | 9550548 |
| [interaction of short oligonucleotides derivatives with nucleic acids. vi. discrimination of mismatch-containing complexes upon ligation of a short oligonucleotide tandem on dna template]. | the high ligation specificity of a tetranucleotide with a pair of flanking octanucleotides on dna template by the action of t4 phage dna ligase is shown. in a tetranucleotide-dna template complex containing a mismatch, almost no ligation products are formed. the ligation of a tandem octanucleotide-tetranucleotide-octanucleotide makes it possible to identify accurately any single nucleotide substitution in a tetranucleotide binding site. | 1998 | 9551199 |
| the cat vertebral column: stance configuration and range of motion. | this study examined the configuration of the vertebral column of the cat during independent stance and in various flexed positions. the range of motion in the sagittal plane is similar across most thoracic and lumbar joints, with the exception of a lesser range at the transition region from thoracic-type to lumbar-type vertebrae. the upper thoracic column exhibits most of its range in dorsiflexion and the lower thoracic and lumbar in ventroflexion. lateral flexion is limited to less than 5 degre ... | 1998 | 9551833 |
| stimulatory effect of melatonin on the 5'-monodeiodinase activity in the liver, kidney, and brown adipose tissue during the early neonatal period of the rabbit. | the response of type i 5'-monodeiodinase activity (5'-md) to a s.c. injection or oral administration of melatonin was studied in 3-, 5-, and 7-day-old rabbits. melatonin-treated animals showed higher activity of the type i 5'-md in the liver and kidney and of type ii 5'-md in brown adipose tissue (bat). this respond to melatonin treatment was age dependent. the stimulatory effect of melatonin on renal 5'-md activity was observed only in 3- and 5-day-old rabbits and in the liver and bat during th ... | 1998 | 9551849 |
| paraplegia following intracord injection during attempted epidural anesthesia under general anesthesia. | a case of permanent paraplegia is reported following attempted epidural anesthesia for a total knee replacement in a 62-year-old woman with a history of lumbar laminectomy for a prolapsed intervertebral disc. | 1998 | 9552788 |
| automated fluorescent analysis procedure for enzymatic mutation detection. | the enzymatic mutation detection (emd) assay detects mutations or polymorphisms in dna. the assay procedure takes <1 h and is followed by electrophoretic detection. we report an automated procedure, using fluorescently labeled probe and quantitative analysis on the abi prism 377 dna sequencer, that improves on earlier methods (1, 2) by eliminating the need for sample purification, shortening the hybridization time, and increasing the signal-to-noise ratio. the emd assay uses the bacteriophage re ... | 1998 | 9554483 |
| nonnasopharyngeal lymphoepithelioma of the head and neck. | lymphoepithelioma (squamous cell carcinoma with associated lymphoid stroma) commonly occurs in the nasopharynx, rarely at other sites. as a result, the clinical course and optimal treatment of nonnasopharyngeal lymphoepithelioma of the head and neck have not been well described. this retrospective study was undertaken to analyze the clinical course of the disease in patients treated at a single institution and to formulate recommendations for treatment based on that experience as well as results ... | 1998 | 9554534 |
| expression of invasion markers cd44v6/v3, nm23 and mmp2 in laryngeal and hypopharyngeal carcinoma. | twelve laryngeal squamous cell carcinoma cases (7 laryngeal and 5 hypopharyngeal cancer; 15 samples) were analysed by immunohistochemistry for the expression of invasion markers cd44v6/v3, nm23 and matrix metalloproteinase, mmp2. the laryngeal epithelium showed cd44v6+/v3+/nm23-/mmp2- phenotype. when tumors were grouped into tnm categories the phenotype of the t2 and t3 tumors was similar, characterised by decreased cd44v3+ and lack of mmp2 expressions. meanwhile the nm23 expression was more fre ... | 1998 | 9555115 |
| [long-term functional outcome of laccourreye hemipharyngectomy-hemilaryngectomy with reference to oncologic outcome]. | various techniques for the treatment of hypopharyngeal malignancies are used to achieve disease control while preserving laryngeal function. | 1998 | 9555703 |
| divergence of a dna replication gene cluster in the t4-related bacteriophage rb69. | the genomes of bacteriophages t4 and rb69 are phylogenetically related but diverge in nucleotide sequence at many loci and are incompatible with each other in vivo. we describe here the biological implications of divergence in a genomic segment that encodes four essential dna replication proteins: gp45 (sliding clamp), gp44/62 complex (clamp loader), and gp46 (a recombination protein). we have cloned, sequenced, and expressed several overlapping segments of the rb69 gene 46-45.2-(rpba)-45-44-62 ... | 1998 | 9555879 |
| chaperones in bacteriophage t4 assembly. | protein folding in the cell is controlled at the levels of translation and post-translational modification, depends on a number of conserved proteins known as chaperones, and is catalyzed by specific enzymes, such as protein disulfide isomerase and peptidyl prolyl cis-trans isomerase. the chaperones stabilize folding intermediates and participate in assembly and disaggregation of supramolecular structures. bacteriophage t4 is an especially convenient system for studying of protein folding mechan ... | 1998 | 9556522 |