Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| molecular characterization of interferon regulatory factor 1 in bubalus bubalis. | interferon regulatory factor 1 (irf1) is functionally diverse in the regulation of immune response and is considered to be an important candidate gene for studying disease susceptibility in mammals. in this paper, we characterized the whole sequence of the irf1 gene in river buffalo (bubalus bubalis) and compared genomic and the amino acid sequences between different species. the buffalo irf1 gene was 7099 bp long and organized into 10 exons and nine introns. its molecular structure showed exact ... | 2015 | 26400319 |
| in the elephant's seed shadow: the prospects of domestic bovids as replacement dispersers of three tropical asian trees. | as populations of the world's largest animal species decline, it is unclear how ecosystems will react to their local extirpation. due to the unique ecological characteristics of megaherbivores such as elephants, seed dispersal is one ecosystem process that may be affected as populations of large animals are decimated. in typically disturbed south asian ecosystems, domestic bovids (cattle, bosprimigenius, and buffalo, bubalus bubalis) may often be the species most available to replace asian eleph ... | 2015 | 26405735 |
| microphthalmia-associated transcription factor mutations are associated with white-spotted coat color in swamp buffalo. | a candidate gene analysis of the microphthalmia-associated transcription factor (mitf) gene was used in an attempt to identify the genetic basis for a white-spotted coat color phenotype in the asian swamp buffalo (bubalus bubalis carabanensis). ninety-three buffaloes-32 solid, 38 spotted and 23 white individuals-were sanger-sequenced for all mitf exons as well as highly conserved intronic and flanking regions. mitf cdna representing skin and iris tissue from six spotted, nine solid and one white ... | 2015 | 26417640 |
| phylogenetic group determination of escherichia coli isolated from animals samples. | this study analyzes the occurrence and distribution of phylogenetic groups of 391 strains of escherichia coli isolated from poultry, cattle, and water buffalo. the frequency of the phylogroups was a = 19%, b1 = 57%, b2 = 2.3%, c = 4.6%, d = 2.8%, e = 11%, and f = 3.3%. phylogroups a (p < 0.001) and f (p = 0.018) were associated with e. coli strains isolated from poultry, phylogroups b1 (p < 0.001) and e (p = 0.002) were associated with e. coli isolated from cattle, and phylogroups b2 (p = 0.003) ... | 2015 | 26421310 |
| molecular characterization of t-cell immunoglobulin mucin domain-3 and galectin-9 genes of swamp- and riverine-type water buffaloes. | molecular characterization of t-cell immunoglobulin mucin domain-3 (tim-3) and galectin-9 (gal-9) genes of swamp- and riverine-type water buffaloes was conducted to compare these genes with other species; determine the unique characteristic specific in water buffalo; and provide baseline information for the assessment of disease progression in buffalo species. tim-3 and gal-9 genes were amplified, purified, sequenced and characterized. the sequence result of tim-3 in both types of water buffaloe ... | 2015 | 26441033 |
| the resurrection of a species: sarcocystis bovifelis heydorn et al., 1975 is distinct from the current sarcocystis hirsuta in cattle and morphologically indistinguishable from sarcocystis sinensis in water buffaloes. | in the mid-1970s, it was established through transmission experiments and ultrastructural studies of sarcocysts by transmission electron microscopy (tem) that cattle was the intermediate host of three sarcocystis spp. using dogs, cats and humans, respectively, as definitive hosts. the cat-transmitted species with microscopic sarcocysts was initially named sarcocystis bovifelis, but it was soon renamed sarcocystis hirsuta, since it was considered to be identical with a previously named species. i ... | 2016 | 26462803 |
| traumatic reticuloperitonitis in water buffalo (bubalus bubalis): clinical findings and the associated inflammatory response. | the present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (trp) in water buffalo (bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. twenty-two buffalo with acute local trp were monitored in our study. additionally, 10 clinically healthy buffalo were randomly selected and served as controls. acute local trp was initially diagnosed by clinical examination and confirmed by ultrasonographic (usg) examina ... | 2013 | 26464911 |
| impact of buserelin acetate or hcg administration on day 5 post-ovulation on subsequent luteal profile and conception rate in murrah buffalo (bubalus bubalis). | the present study aimed to establish the impact of buserelin acetate or hcg administration on day 5 post-ovulation on subsequent luteal profile and conception rate in buffalo. the buffalo (n=45) were subjected to an estrous synchronization protocol (synthetic analog of pgf2α administered, through intramuscular route, 11 days apart), followed by artificial insemination (ai) during mid to late estrus. on day 5 post-ovulation, buffalo were administered (i.m.) normal saline (control, n=14), busereli ... | 2015 | 26471839 |
| characterization and annotation of babesia orientalis apicoplast genome. | babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (bubalus bubalis, linnaeus, 1758) transmitted by the tick rhipicephalus heamaphysaloides. it is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern china. as a member of the phylum apicomplexa, b. orientalis possesses a relatively independent and alga originated organelle the apicoplast. apicoplasts in other apicomplexa parasites are invo ... | 2015 | 26474853 |
| buffalo (bubalus bubalis) scnt embryos produced from somatic cells isolated from frozen-thawed semen: effect of trichostatin a on the in vitro and in vivo developmental potential, quality and epigenetic status. | this study examined the effects of trichostatin a (tsa) treatment of reconstructed buffalo embryos, produced by hand-made cloning using somatic cells isolated from over a decade old frozen-thawed semen, on their in vitro and in vivo developmental competence, quality and epigenetic status. following treatment of reconstructed embryos with tsa (0, 50 or 75 nm) for 10 h prior to culture, the cleavage (100.0 ± 0, 94.5 ± 2.3 and 96.1 ± 1.2%, respectively) and blastocyst rate (50.6 ± 2.3, 48.4 ± 2.7 a ... | 2016 | 26503476 |
| differentiated evolutionary relationships among chordates from comparative alignments of multiple sequences of myod and myog myogenic regulatory factors. | myod and myog are transcription factors that have essential roles in myogenic lineage determination and muscle differentiation. the purpose of this study was to compare multiple amino acid sequences of myogenic regulatory proteins to infer evolutionary relationships among chordates. protein sequences from mus musculus (p10085 and p12979), human homo sapiens (p15172 and p15173), bovine bos taurus (q7ys82 and q7ys81), wild pig sus scrofa (p49811 and p49812), quail coturnix coturnix (p21572 and p34 ... | 2015 | 26505406 |
| prospecting major genes in dairy buffaloes. | asian buffaloes (bubalus bubalis) have an important socio-economic role. the majority of the population is situated in developing countries. due to the scarce resources in these countries, very few species-specific biotechnology tools exist and a lot of cattle-derived technologies are applied to buffaloes. however, the application of cattle genomic tools to buffaloes is not straightforward and, as results suggested, despite genome sequences similarity the genetic polymorphisms are different. | 2015 | 26510479 |
| actin polymerization: an event regulated by tyrosine phosphorylation during buffalo sperm capacitation. | in the female reproductive tract, the spermatozoa undergo a series of physiological and biochemical changes, prior to gaining the ability to fertilize, that result to capacitation. however, the actin polymerization and protein tyrosine phosphorylation are the two necessary steps for capacitation. in this study, we have demonstrated the actin polymerization and established the correlation between protein tyrosine phosphorylation and actin reorganization during in vitro capacitation in buffalo (bu ... | 2015 | 26514336 |
| morphologic and genotypic characterization of psoroptes mites from water buffaloes in egypt. | species delimitation of psoroptes spp. and identity of the parasite in water buffaloes remain poorly defined. in this study, psoroptes infestation on three water buffalo farms in egypt was examined based on morphometric characteristics, especially the opisthosomal setae of adult male mites. clinical investigations showed that 28% (196/700) of the sampled animals had mange infestation. microscopic examinations of 80 skin scrapings indicated the occurrence of psoroptes mites in 17 (21.3%) samples, ... | 2015 | 26517834 |
| optixcell improves the postthaw quality and fertility of buffalo bull sperm. | the present study was conducted to compare the liposome-containing, animal protein-free, commercially available optixcell extender with the tris-citric-egg yolk extender for postthaw quality and fertility of buffalo semen. semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with an artificial vagina (at 42 °c) for 3 weeks (replicates). semen ejaculates from each buffalo bull were divided into two aliquots and diluted (at 37 °c having 50 × 10(6) sper ... | 2016 | 26522702 |
| fatal theileria orientalis n2 genotype infection among asian water buffaloes (bubalus bubalis) in a commercial dairy farm in kerala, india. | fifteen dairy buffaloes of a farm in the state of kerala, india developed fatal oriental theileriosis within 2 months of their procurement. typical piroplasms of theileria orientalis were observed in the erythrocytes of all affected animals by giemsa-leishman staining of blood smears. case fatality rate was 87·5% (seven out of eight) in the clinically progressed cases. therapeutic management with anti-theilerial drugs buparvaquone and oxytetracycline led to recovery of seven other animals in les ... | 2016 | 26522773 |
| retracted: bone morphogenetic protein 4 (bmp4) induces buffalo (bubalus bubalis) embryonic stem cell differentiation into germ cells. | the aim of the present study was to investigate the effect of bone morphogenetic protein 4 (bmp4) stimulation on differentiation of buffalo embryonic stem (es) cells into germ lineage cells. es cells were subjected to in vitro differentiation in floating and adherent cultures, under different bmp4 concentrations (20, 50 and 100 ngml(-1)) for different culture intervals (4, 8 and 14 days). qpcr analysis revealed that bmp4 at a concentration of 50-100 ngml(-1) for a culture period of 14 days led t ... | 2015 | 26529649 |
| intraspecific and interspecific genetic variation of gongylonema pulchrum and two rodent gongylonema spp. (g. aegypti and g. neoplasticum), with the proposal of g. nepalensis n. sp. for the isolate in water buffaloes from nepal. | the gullet worm (gongylonema pulchrum) has been recorded from a variety of mammals worldwide. in an earlier study, we demonstrated two separate transmission cycles in cattle (bos taurus) and wild mammals in japan based on nucleotide sequences of the ribosomal rna gene (rdna) and cytochrome c oxidase subunit i (cox-1) region of mitochondrial dna of multiple isolates of different origins. our earlier study additionally demonstrated two major cox-1 haplotypes of g. pulchrum prevalent in cattle in j ... | 2016 | 26531300 |
| a framework radiation hybrid map of buffalo chromosome 1 ordering scaffolds from buffalo genome sequence assembly. | river buffalo chromosome 1 (bbu1) is a sub-metacentric chromosome homologous to bovine chromosomes 1 and 27. in this study, we constructed a new framework radiation hybrid (rh) map from bbu1 using bburh5000 panel adding nine new genes (adrb3, atp2c1, copb2, crygs, p2ry1, slc5a3, slc20a2, sst, and zdhhc2) and one microsatellite (cssm043) to the set of markers previously mapped on bbu1. the new framework rh map of bbu1 contained 141 markers (55 genes, 2 ests, 10 microsatellites, and 74 snps) distr ... | 2015 | 26535622 |
| antimicrobial effect of malpighia punicifolia and extension of water buffalo steak shelf-life. | in the present study, a multiple approach was used to characterize malpighia punicifolia extract and to evaluate its inhibitory activity against several meat spoilage bacteria. first, volatile fraction, vitamins and phenolic compounds of the extract obtained by supercritical fluid extraction were determined by gc-ms and hplc. then, the antimicrobial action of the extract was in vitro evaluated against pseudomonas putida dsmz 291(t), pseudomonas fluorescens dsmz 50009(t), pseudomonas fragi dsmz 3 ... | 2015 | 26556435 |
| supplementation of slow-release melatonin improves recovery of ovarian cyclicity and conception in summer anoestrous buffaloes (bubalus bubalis). | the role of melatonin as a protective neurohormone against restoring cyclicity in summer anoestrous animals in photoperiod species has gained wider acceptance. this study was designed to uncover the evidence the slow-release melatonin (mlt) has on initiation of ovarian cyclicity and conception rate (cr) in summer anoestrous buffaloes. thus, buffaloes diagnosed as summer anoestrous (absence of overt signs of oestrus, concurrent rectal examination and radioimmunoassay for serum progesterone at 10 ... | 2016 | 26566713 |
| dispositions of enrofloxacin and its major metabolite ciprofloxacin in thai swamp buffaloes. | given the limited information available in this species, the aim of this study was to investigate the pharmacokinetic characteristics of enrofloxacin (er) and its major metabolite ciprofloxacin (cp) in buffaloes, bubalus bubalis. er was administered intravenously (i.v.) or subcutaneously (s.c.) to buffaloes at doses of 5.0 and 7.5 mg/kg bw, and plasma, urine and fecal samples were collected until 48 hr post-administration. the concentrations of er and cp in the plasma, urine and feces were analy ... | 2016 | 26596287 |
| profile of muscle tissue gene expression specific to water buffalo: comparison with domestic cattle by genome array. | in contrast with the past, the water buffalo is now not only a draft animal, but also an important food source of milk and meat. it is increasingly apparent that the water buffalo have huge potential for meat production, but its breeding needs to be investigated. regarding the molecular mechanisms involved in the meat quality difference between the buffalo (bubalus bulabis) and yellow cattle (bos taurus), 12 chemical-physical characteristics related to the meat quality of longissimus thoracis mu ... | 2016 | 26598327 |
| loss of heat shock protein 70 from apical region of buffalo (bubalus bubalis) sperm head after freezing and thawing. | the post-thaw fertility of frozen-thawed mammalian spermatozoa is substantially low as compared with that of fresh sperm. furthermore, the post-thaw fertility of the cryopreserved buffalo sperm has been reported to be poor as compared with that of cattle sperm. recently, heat shock protein 70 (hsp70) has been found to play a critical role in mammalian fertilization and early embryonic development in boar and cattle. however, the presence of such fertility-related hsp70 in buffalo sperm and its s ... | 2016 | 26607876 |
| magnetic resonance imaging and computer tomography of brain lesions in water buffaloes and cattle stunned with handguns or captive bolts. | owing to the demand for genuine mozzarella, some 330 water buffaloes are being slaughtered every year in switzerland albeit a stunning procedure meeting animal welfare and occupational safety requirements remains to be established. to provide a basis for improvements, we sized anatomical specifics in water buffaloes and cattle and we assessed brain lesions after stunning with captive bolts or handguns by diagnostic imaging. in water buffaloes and cattle, the median distance from the frontal skin ... | 2016 | 26610289 |
| identification of functional consequence of a novel selection signature in cyp11b1 gene for milk fat content in bubalus bubalis. | genomic selection for traits of economic importance is an emerging approach carrying tremendous potentials. many of polygenic traits as milk fat, protein and yield have been characterize at genomic level and important selection signatures have been identified. cytochrome p450 enzymes are potential loci for affecting many of dairy capabilities. present study was conducted for genomic dissection of cyp11b1 gene in riverine buffaloes and seven genetic variations were identified. out of these, one n ... | 2015 | 26629413 |
| activation and inhibition of the wnt3a signaling pathway in buffalo (bubalus bubalis) embryonic stem cells: effects of wnt3a, bio and dkk1. | this research studies the effects of activation and inhibition of wnt3a signaling pathway in buffalo (bubalus bubalis) embryonic stem (es) cell-like cells. | 2015 | 26644860 |
| molecular characterization of buffalo haptoglobin: sequence based structural comparison indicates convergent evolution between ruminants and human. | haptoglobin (hp) protein has high affinity for hemoglobin (hb) binding during intravascular hemolysis and scavenges the hemoglobin induced free radicals. earlier reports indicate about uniqueness of hp molecule in human and cattle, but in other animals, it is not much studied. in this paper, we characterized buffalo hp molecule and determined its molecular structure, evolutionary importance, and tissue expression. comparative analysis and predicted domain structure indicated that the buffalo hp ... | 2016 | 26646629 |
| trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in nili ravi buffaloes (bubalus bubalis). | our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) in vitro quality (after thawing), and (3) in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 20) from four buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mm) and frozen in french straws. at ... | 2016 | 26653954 |
| differential developmental competence and gene expression patterns in buffalo (bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells. | the developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (bubalus bubalis) nuclear transfer (nt) embryos from fetal fibroblasts (ffs), amnion mesenchymal stem cells (amscs) and in vitro fertilized (ivf) embryos were compared in the present studies. the in vitro expanded buffalo ffs showed a typical "s" shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. the in vitro cultured undifferentiated amscs showed a doubling t ... | 2016 | 26660476 |
| amnion epithelial cells of buffalo (bubalus bubalis) term placenta expressed embryonic stem cells markers and differentiated into cells of neurogenic lineage in vitro. | aim of the present study was the isolation, culture, and characterization of amniotic membrane-derived epithelial cells (ae) from term placenta collected postpartum in buffalo. we found that cultured cells were of polygonal in shape, resistance to trypsin digestion and expressed cytokeratin-18 indicating that they were of epithelial origin. these cells have negative expression of mesenchymal stem cell markers (cd29, cd44, and cd105) and positive for pluripotency marker (oct4) genes indicated tha ... | 2016 | 26670951 |
| mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat italian ricotta cheese. | ricotta cheese is a typical italian product, made with whey from various species, including cow, buffalo, sheep, and goat. ricotta cheese nominally manufactured from the last three species may be fraudulently produced using the comparatively cheaper cow whey. exposing such food frauds requires a reliable analytical method. despite the extensive similarities shared by whey proteins of the four species, a mass spectrometry-based analytical method was developed that exploits three species-specific ... | 2016 | 26675863 |
| risk analysis and seroprevalence of bovine ephemeral fever virus in cattle in the kingdom of saudi arabia. | bovine ephemeral fever virus (befv) is an arthropod-borne rhabdovirus that causes disabling clinical signs and major economic losses in cattle and water buffalo. the disease is well documented in asia, africa, and the middle east; however, the seroprevalence of befv in different regions and bovine breeds in the kingdom of saudi arabia (ksa) is unknown. the aim of this study was to analyze risk factors which affect the prevalence of antibodies against befv in small herds of cattle in four geograp ... | 2016 | 26676243 |
| strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the china/indochina border region. | the swamp type of the asian water buffalo is assumed to have been domesticated by about 4000 years bp, following the introduction of rice cultivation. previous localizations of the domestication site were based on mitochondrial dna (mtdna) variation within china, accounting only for the maternal lineage. we carried out a comprehensive sampling of china, taiwan, vietnam, laos, thailand, nepal and bangladesh and sequenced the mtdna cytochrome b gene and control region and the y-chromosomal zfy, sr ... | 2016 | 26677084 |
| molecular characterisation of sarcocystis bovifelis, sarcocystis bovini n. sp., sarcocystis hirsuta and sarcocystis cruzi from cattle (bos taurus) and sarcocystis sinensis from water buffaloes (bubalus bubalis). | about 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries (argentina, brazil, germany, new zealand, uruguay) and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology. genomic dna was extracted from 147 of these sarcocysts and used initially for pcr amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit i gene (cox1) in order to identify the sarcocysts to species and/or se ... | 2016 | 26677095 |
| genome sequences of 11 brucella abortus isolates from persistently infected italian regions. | bovine brucellosis, typically caused by brucella abortus, has been eradicated from much of the developed world. however, the disease remains prevalent in southern italy, persisting as a public and livestock health concern. we report here the whole-genome sequences of 11 isolates from cattle (bos taurus) and water buffalo (bubalus bubalis) that are representative of the current genetic diversity of b. abortus lineages circulating in italy. | 2015 | 26679575 |
| generation of a novel bacteriophage library displaying scfv antibody fragments from the natural buffalo host to identify antigens from adult schistosoma japonicum for diagnostic development. | the development of effective diagnostic tools will be essential in the continuing fight to reduce schistosome infection; however, the diagnostic tests available to date are generally laborious and difficult to implement in current parasite control strategies. we generated a series of single-chain antibody fv domain (scfv) phage display libraries from the portal lymph node of field exposed water buffaloes, bubalus bubalis, 11-12 days post challenge with schistosoma japonicum cercariae. the select ... | 2015 | 26684756 |
| detection of sarcocystis spp. in cattle (bos taurus) and water buffaloes (bubalus bubalis) in iran by pcr-rflp. | sarcocystis species are cyst-forming intracellular protozoan parasites. cattle are mainly infected with sarcocystis cruzi, sarcocystis hominis and sarcocystis hirsuta. water buffaloes are intermediate hosts for sarcocystis fusiformis, sarcocystis levinei (s. cruzi-like species), sarcocystis dubeyi, sarcocystis sinensis (s. hominis-like species) and sarcocystis buffalonis (s. hirsuta- like species). the aim of this study was identification of sarcocystis spp. in slaughtered cattle and water buffa ... | 2014 | 26688630 |
| the effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using mehsani buffalo (bubalus bubalis). | in developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. it is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. a metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic mehsani buffalo (bubalus bubalis) fed with varying proportions of roughage. the ex ... | 2016 | 26711277 |
| effect of roughage on rumen microbiota composition in the efficient feed converter and sturdy indian jaffrabadi buffalo (bubalus bubalis). | the rumen microbiota functions as an effective system for conversion of dietary feed to microbial proteins and volatile fatty acids. in the present study, metagenomic approach was applied to elucidate the buffalo rumen microbiome of jaffrabadi buffalo adapted to varied dietary treatments with the hypothesis that the microbial diversity and subsequent in the functional capacity will alter with diet change and enhance our knowledge of effect of microbe on host physiology. eight adult animals were ... | 2015 | 26714477 |
| biochemical, immunological and kinetic characterization and partial sequence analysis of a thiol proteinase inhibitor from bubalus bubalis kidney: an attempt targeting kidney disorders. | in the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use of ammonium sulphate precipitation and gel filtration chromatography on sephacryl s-100hr column. purified inhibitor is homogeneous as it displayed a single band in gel electrophoresis both under reducing and non-reducing environment and is of 65kda as revealed by gel filtration and sds page. kinetic studies revealed the presence of reversible accompanied with competitive mode of inhibition; showing max ... | 2017 | 26751400 |
| first report of cryptosporidium species in farmed and wild buffalo from the northern territory, australia. | a molecular epidemiological survey of cryptosporidium from water buffalo (bubalus bubalis) in the northern territory in australia was conducted. fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18s quantitative pcr (qpcr). positives were typed by sequence analysis of 18s nested pcr products. the qpcr prevalence of cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. sequence analysis identified two species: c. pa ... | 2016 | 26758449 |
| l-cysteine improves antioxidant enzyme activity, post-thaw quality and fertility of nili-ravi buffalo (bubalus bubalis) bull spermatozoa. | the effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of nili-ravi buffalo bull spermatozoa were studied. semen samples from 4 buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml french straws. the antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase ... | 2016 | 26762772 |
| de novo transcriptome assembly of the chinese swamp buffalo by rna sequencing and ssr marker discovery. | the chinese swamp buffalo (bubalis bubalis) is vital to the lives of small farmers and has tremendous economic importance. however, a lack of genomic information has hampered research on augmenting marker assisted breeding programs in this species. thus, a high-throughput transcriptomic sequencing of b. bubalis was conducted to generate transcriptomic sequence dataset for gene discovery and molecular marker development. illumina paired-end sequencing generated a total of 54,109,173 raw reads. af ... | 2016 | 26766209 |
| evaluation of analgesic effect of caudal epidural tramadol, tramadol-lidocaine, and lidocaine in water buffalo calves (bubalus bubalis). | aim of this study was to compare the analgesic effect of tramadol and a combination of tramadol-lidocaine with that produced by lidocaine administration in the epidural space in buffalo calves. in a prospective randomized crossover study, ten male buffalo calves were used to compare the epidural analgesic effect of tramadol (1 mg/kg) and tramadol-lidocaine combination (0.5 mg/kg and 0.11 mg/kg, resp.) with that produced by 2% lidocaine (0.22 mg/kg). loss of sensation was examined by pin-prick te ... | 2015 | 26770870 |
| rapid detection of water buffalo ricotta adulteration or contamination by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. | the improvement and development of novel technologies and analytical tools are an important first line of defence for both detecting and deterring food frauds. in order to protect the authenticity of traditional foods and safeguard geographical indications and traditional specialities at european level, protected designation of origin (pdo) legislation has been introduced as a guarantee of quality (council regulation ecc 2081/92). dairy products, especially those certified as pdo products, are a ... | 2016 | 26777680 |
| heat stress and antioxidant enzyme activity in bubaline (bubalus bubalis) oocytes during in vitro maturation. | in vitro environments like heat stress usually increase the production of reactive oxygen species in bubaline oocytes which have been implicated as one of the major causes for reduced developmental competence. oocytes during meiotic maturation are sensitive to oxidative stress, and heat stress accelerates cellular metabolism, resulting in the higher production of free radicals. therefore, the aim of present work was to assess the impact of heat stress during meiotic maturation on bubaline cumulu ... | 2016 | 26781547 |
| comparative proteomic analysis of mature and immature oocytes of the swamp buffalo (bubalus bubalis). | maternal protein components change markedly during mammalian oogenesis. many of these proteins have yet to be characterized and verified. in this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the swamp buffalo (bubalus bubalis). proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. image analysis indicated that 17 proteins were differentially express ... | 2016 | 26784167 |
| short communication: role of streptococcus pluranimalium in mediterranean buffaloes (bubalus bubalis) with different udder health statuses. | the aims of the current study were to describe presence and clinical role over time of streptococcus pluranimalium isolated in milk samples of mediterranean buffalo (mb). two hundred composite milk samples originating from 40 primiparous mb were collected at 10, 30, 60, 90, and 150d in milk (dim) and from 20 pluriparous mb at 77 to 120 dim. milk samples were used for analysis of somatic cell counts, bacteriological cultures, and identification (matrix-assisted laser desorption/ionization time-of ... | 2016 | 26805969 |
| caspase-9 inhibitor z-lehd-fmk enhances the yield of in vitro produced buffalo (bubalus bubalis) pre-implantation embryos and alters cellular stress response. | the present investigation was done to study the effect of caspase-9 inhibitor z-lehd-fmk, on in vitro produced buffalo embryos. z-lehd-fmk is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival. buffalo ovaries were collected from slaughterhouse and the oocytes were subjected to in vitro maturation (ivm), in vitro fertilization (ivf) and in vitro culture (ivc). the culture medium was supplemented with z-lehd-fmk at different concentrations i ... | 2016 | 26850530 |
| investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis). | the aim of this study was to describe modifications in plasma homocysteine (hcy), vitamin e (vite) and malondialdehyde (mda) concentrations in the first 56 days after artificial insemination (ai) in buffalo. thirty-five buffalo cows were divided, ex post, into three groups on the basis of pregnancy diagnosis: pregnant, not pregnant, with embryonic mortality. pregnancy was diagnosed by ultrasonography and plasma concentrations of pregnancy-associated glycoproteins (pags). our results showed that, ... | 2016 | 26850546 |
| involvement of the nervous system following experimental infection with pasteurella multocida b:2 in buffalo (bubalus bubalis): a clinicopathological study. | haemorrhagic septicaemia (hs) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of pasteurella multocida b:2 and e:2 in asian and african, respectively. it is well known that hs affect mainly the respiratory and digestive tracts. however, involvement of the nervous system in pathogenesis of hs has been reported in previous studies without details. in this study, nine buffalo calves of 8 months old were distributed into three groups. animals o ... | 2016 | 26850845 |
| evidence of fasciola spp. resistance to albendazole, triclabendazole and bromofenofos in water buffaloes (bubalus bubalis). | fasciolosis caused by fasciola spp. is considered the most important helminth infection of ruminants in tropical countries. anthelmintic resistance has become a global concern. this study compared the efficacy of the commonly used anthelmintics, determined the toxicity level and any indication of resistance. thirty two water buffaloes naturally-infected with fasciola spp. were used to determine the efficacy of triclabendazole (tbz), albendazole (abz), and bromofenofos (bro) using fecal egg count ... | 2015 | 26878627 |
| combined il-12 plasmid and recombinant sjgst enhance the protective and anti-pathology effect of sjgst dna vaccine against schistosoma japonicum. | schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. development of a vaccine is thought to be the most effective way to control this disease. recombinant 26-kda glutathione s-transferase (rsjgst) has previously been reported to achieve a worm reduction rate of 42-44%. to improve the efficiency of the vaccine against schistosoma japonicum, we immunized mice with a combination of pcdna vector-encoded 26-kda sjgst (pcdn ... | 2016 | 26891172 |
| isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to investigate the occurrence of bacterial species in water buffalo semen at the time of collection/processing and to assess the efficacy of some selected antibiotics (gtls; gentamycin, tylosin and linco-spectin or sp; streptomycin and penicillin) in cryodiluent on bacterial control and quality including in vivo fertility of buffalo spermatozoa. for this purpose, four experiments were conducted. in experiment 1, a total of 11 bacterial species were isolated from buffalo e ... | 2016 | 26892806 |
| identification of polymorphism in fatty acid binding protein 3 (fabp3) gene and its association with milk fat traits in riverine buffalo (bubalus bubalis). | the fatty acid binding protein 3 (fabp3) gene, known to be associated with fat percentage of milk and meat in bovines, was screened among swamp and riverine buffaloes for polymorphism detection and further association with milk fat contents. an snp g.307c > t was identified in the intron 2 (+53 exon 2) region of fabp3 gene of indian buffaloes. the snp identified was genotyped in 692 animals belonging to 15 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes an ... | 2016 | 26894500 |
| computational studies on receptor-ligand interactions between novel buffalo (bubalus bubalis) nucleotide-binding oligomerization domain-containing protein 2 (nod2) variants and muramyl dipeptide (mdp). | nucleotide binding and oligomerization domain 2 (nod2), a member of intracellular nod-like receptors (nlrs) family, recognizes the bacterial peptidoglycan, muramyl dipeptide (mdp) and initiates host immune response. the precise ligand recognition mechanism of nod2 has remained elusive, although studies have suggested leucine rich repeat (lrr) region of nod2 as the possible binding site of mdp. in this study, we identified multiple transcripts of nod2 gene in buffalo (bunod2) and at least five lr ... | 2016 | 26897084 |
| influence of cutting and deboning operations on the microbiological quality and shelf life of buffalo meat. | considering the specific biochemical composition of buffalo (bubalus bubalis) meat (high iron content, high biological value proteins and essential fatty acids, low amounts of fat and cholesterol), we evaluated the influence of cutting and deboning operations on the microbiological quality and shelf-life of vacuum-packed buffalo meat stored under refrigeration. on the processing day, samples were collected from carcass, deboning room surfaces and meat cuts. samples from meat cuts were evaluated ... | 2016 | 26897086 |
| sequence analysis of the s1pr1 gene in river buffalo. | recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. the s1pr1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. here, we performed next generation sequencing in a buffalo bac dna clone and obtained a 54.5-kb sequence encompassing the entire buffalo s1pr1 gene as well as the 27 kb upstream region ... | 2016 | 26909928 |
| exploring genetic polymorphism in innate immune genes in indian cattle (bos indicus) and buffalo (bubalus bubalis) using next generation sequencing technology. | activation of innate immunity initiates various cascades of reactions that largely contribute to defense against physical, microbial or chemical damage, prompt for damage repair and removal of causative organisms as well as restoration of tissue homeostasis. genetic polymorphism in innate immune genes plays prominent role in disease resistance capabilities in various breeds of cattle and buffalo. here we studied single nucleotide variations (snp/snv) and haplotype structure in innate immune gene ... | 2015 | 26925373 |
| isolation and identification of prepubertal buffalo (bubalus bubalis) spermatogonial stem cells. | isolation and culture of spermatogonial stem cells (sscs) are attractive for production of genetic modified offspring. in the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. to recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. two types of cells, immature sertoli cell and type a spermatogonia were obser ... | 2016 | 26954139 |
| molecular differentiation of sarcocystis buffalonis and sarcocystis levinei in water buffaloes (bubalus bubalis) from sarcocystis hirsuta and sarcocystis cruzi in cattle (bos taurus). | the purpose of the present study was to obtain sarcocysts of sarcocystis buffalonis and sarcocystis levinei from water buffaloes and characterize the isolates by molecular methods in order to determine whether the two species were genetically different from sarcocystis hirsuta and sarcocystis cruzi, respectively, from cattle, which had been characterized before. about 35 macroscopically visible (3-4 × 1-2 mm) and 20 barely visible (1-3 × 0.2 mm) sarcocysts were excised from the esophagus of 18 n ... | 2016 | 26979729 |
| development of buffalo (bubalus bubalis) embryonic stem cell lines from somatic cell nuclear transferred blastocysts. | we developed buffalo embryonic stem cell lines from somatic cell nuclear transfer derived blastocysts, produced by hand-guided cloning technique. the inner cell mass of the blastocyst was cut mechanically using a microblade and cultured onto feeder cells in buffalo embryonic stem (es) cell culture medium at 38 °c in a 5% co2 incubator. the stem cell colonies were characterized for alkaline phosphatase activity, karyotype, pluripotency and self-renewal markers like oct4, nanog, sox2, c-myc, foxd3 ... | 2015 | 26987926 |
| detection of campylobacter in human and animal field samples in cambodia. | campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ... | 2016 | 26991032 |
| ungulate malaria parasites. | haemosporida parasites of even-toed ungulates are diverse and globally distributed, but since their discovery in 1913 their characterization has relied exclusively on microscopy-based descriptions. in order to bring molecular approaches to bear on the identity and evolutionary relationships of ungulate malaria parasites, we conducted plasmodium cytb-specific nested pcr surveys using blood from water buffalo in vietnam and thailand, and goats in zambia. we found that plasmodium is readily detecta ... | 2016 | 26996979 |
| no change in mrna expression of immune-related genes in peripheral blood mononuclear cells challenged with theileria annulata in murrah buffalo (bubalus bubalis). | water buffaloes (bubalus bubalis) act as carrier to theileria annulata and show less clinical sign of tropical theileriosis as compared to indigenous and exotic cattle. differential expression of immune-related genes such as major histocompatibility complex, class ii, dq alpha 1 (mhc-dqα), signal-regulatory protein alpha (sirpa), prion protein (prnp), toll-like receptor 10 (tlr10), c-musculoaponeurotic fibrosarcoma oncogene homolog (cmaf) and v-maf avian musculoaponeurotic fibrosarcoma oncogene ... | 2016 | 26997138 |
| inhibitory potential of buffalo (bubalus bubalis) colostrum immunoglobulin g on klebsiella pneumoniae. | the unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. in this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin g on human pathogen klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. the ... | 2016 | 27017977 |
| how livestock and flooding mediate the ecological integrity of working forests in amazon river floodplains. | the contribution of working forests to tropical conservation and development depends upon the maintenance of ecological integrity under ongoing land use. assessment of ecological integrity requires an understanding of the structure, composition, and function and major drivers that govern their variability. working forests in tropical river floodplains provide many goods and services, yet the data on the ecological processes that sustain these services is scant. in flooded forests of riverside am ... | 2016 | 27039519 |
| effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (bubalus bubalis) oocytes in vitro. | the aim was to assess the effects of fetal bovine serum (fbs) and estrus buffalo serum (ebs) on in vitro maturation rate of oocytes in buffalo. | 2015 | 27047063 |
| fertility response in postpartum anoestrus buffaloes (bubalus bubalis) using modified ovsynch based timed insemination protocols. | suboestrus constitutes the largest factor responsible for poor reproductive efficiency in buffaloes. therefore, oestrus synchronization (os) and fixed-time artificial insemination (ai) is considered an alternative approach to enhance reproductive efficiency in buffaloes. thus, the present study was carried out to study the efficacy of modified ovsynch protocol with fixed time insemination in post-partum anoestrus buffaloes. | 2015 | 27047090 |
| evaluation of biochemical alterations produced by acetaminophen overdose in bubalus bubalis. | evaluation of the effect of acetaminophen (apap) overdose on biochemical parameters in buffalo calves. | 2015 | 27047122 |
| hemato-biochemical and hormonal profiles in post-partum water buffaloes (bubalus bubalis). | the objective of the present study was to compare serum as well as follicular fluid (ff) biochemical and hormonal profiles along with hematological parameters in postpartum estrus, anestrus, and cystic buffaloes. | 2015 | 27047125 |
| comparison of follicular dynamics, superovulatory response, and embryo recovery between estradiol based and conventional superstimulation protocol in buffaloes (bubalus bubalis). | to evaluate the follicular dynamics, superovulatory response, and embryo recovery following superstimulatory treatment initiated at estradiol-17β induced follicular wave emergence and its comparison with conventional superstimulatory protocol in buffaloes. | 2015 | 27047186 |
| effect of age, sex and physiological stages on hematological indices of banni buffalo (bubalus bubalis). | to determine the physiological baseline values for hematological indices of banni buffalo (bubalus bubalis) as well as to assess their alteration due to age, sex and physiological stages. | 2016 | 27051182 |
| effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (bubalus bubalis) spermatozoa. | the aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (clc) treated buffalo spermatozoa. | 2016 | 27051205 |
| ultrastructural changes in the sublingual salivary gland of prenatal buffalo (bubalus bubalis). | the present study was aimed to elucidate ultrastructural changes in the development of sublingual salivary gland of buffalo during prenatal life. | 2016 | 27057120 |
| domestic cats (felis catus) are definitive hosts for sarcocystis sinensis from water buffaloes (bubalus bubalis). | the definitive hosts of sarcocystis sinensis in water buffaloes have hitherto been unknown, but the close similarity of this species to the cat-transmitted sarcocystis bovifelis in cattle suggested they were felids. in a previous study, two domestic cats were fed macroscopic sarcocysts of sarcocystis fusiformis contained within or dissected from the esophageal muscles of water buffaloes, while no microscopic sarcocysts of s. sinensis were noticed. both cats started shedding small numbers of spor ... | 2016 | 27075117 |
| understanding tenderness variability and ageing changes in buffalo meat: biochemical, ultrastructural and proteome characterization. | understanding of biological impact of proteome profile on meat quality is vital for developing different approaches to improve meat quality. present study was conducted to unravel the differences in biochemical, ultrastructural and proteome profile of longissimus dorsi muscle between buffaloes (bubalus bubalis) of different age groups (young v. old). higher (p<0.05) myofibrillar and total protein extractability, muscle fibre diameter, and warner-bratzler shear force (wbsf) values was observed in ... | 2016 | 27076348 |
| anti-mullerian hormone (amh) concentration in follicular fluid and mrna expression of amh receptor type ii and lh receptor in granulosa cells as predictive markers of good buffalo (bubalus bubalis) donors. | high individual variability in follicular recruitment and hence in the number of embryos produced is a major factor limiting the application of reproductive technologies in buffalo. therefore, the identification of reliable markers to select embryo donors is critical to enroll buffaloes in embryo production programs. better understanding of factors involved in follicular growth is also necessary to improve the response to superovulation in this species. the aim of this work was thus to determine ... | 2016 | 27087535 |
| expression of putative stem cell marker, hepatocyte nuclear factor 4 alpha, in mammary gland of water buffalo. | buffaloes account for more than 56% of total milk production in india. cyclic remodeling of mammary glands of human, mice, cow, sheep, and goat is determined by mammary stem cells. it is logical to assume that buffalo mammary gland will have mammary stem/progenitor cells. thus far, no report exists on identification of buffalo mammary stem cells. hepatocyte nuclear factor 4 alpha (hnf4a) is a candidate marker for hepatic progenitor cells and has recently been suggested as a marker of bovine mamm ... | 2016 | 27092988 |
| flow cytometric and near-infrared raman spectroscopic investigation of quality in stained, sorted, and frozen-thawed buffalo sperm. | flow cytometry and laser tweezers raman spectroscopy have been used to investigate nili-ravi buffalo (bubalus bubalis) sperm from different samples (fresh, stained, sorted and frozen-thawed) of the flow-sorting process to optimize sperm sex sorting procedures. during the sorting and freezing-thawing processes, the two detection methods both indicated there were differences in mitochondrial activity and membrane integrity. moreover, a dispersive-type nir (near infrared reflection) use of the rama ... | 2016 | 27095613 |
| rumen adaptation of swamp buffaloes (bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet. | four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly allocated to investigate rumen adaptation of urea on feed intake, nutrient digestibility, fermentation efficiency, and microbial protein synthesis. buffaloes were fed with rice straw ad libitum for a period of 2 weeks and then were shifted to a step-up diet regimen by supplementation of concentrate containing 20 and 40 g/kg urea at 5 g/kg bw for a period of 2 and 4 weeks, respectively. the results revealed that feed intake and ... | 2016 | 27107749 |
| microorganisms in the rumen and reticulum of buffalo (bubalus bubalis) fed two different feeding systems. | the community of microorganisms in the rumen and reticulum is influenced by feeding as well as the species and geographical distribution of ruminant animals. bacteria, methanogenic archaea and ciliate protozoa existing in the rumen and reticulum were evaluated by real-time polymerase chain reaction and light microscopy in buffalo in two feeding systems, grazing and feedlot. | 2016 | 27118203 |
| water buffalo (bubalus bubalis) as a spontaneous animal model of vitiligo. | vitiligo is a multifactorial acquired depigmenting disorder. recent insights into the molecular mechanisms driving the gradual destruction of melanocytes in vitiligo will likely lead to the discovery of novel therapies, which need to be evaluated in animal models that closely recapitulate the pathogenesis of human vitiligo. in humans, vitiligo is characterized by a spontaneous loss of functional melanocytes from the epidermis, but most animal models of vitiligo are either inducible or geneticall ... | 2016 | 27124831 |
| embryonic and fetal mortality in river buffalo (bubalus bubalis). | river buffalo are able to adapt to diverse climatic zones and are widespread globally. the resource use efficiency of buffalo is highly relevant in a resource-constrained world and the increasing requirement to produce more food. buffalo clearly have an important role in meeting the growing demand for animal protein. in the mediterranean and higher latitudes, buffalo show annual cycles of ovarian activity, embryonic development, and pregnancy rate. in buffalo, the cl starts to develop early in t ... | 2016 | 27142486 |
| epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in vietnam. | a pcr-based survey of hemoprotozoa parasites detected babesia bigemina, theileria orientalis and trypanosoma theileri among cattle and water buffalo in vietnam, and a new babesia sp. closely related to babesia ovata was detected in cattle only. in addition, theileria annulata and trypanosoma evansi were not detected in both cattle and water buffalo. phylogenetic analysis detected t. orientalis mpsp genotypes 3, 5, 7 and n3 in cattle and 5, 7, n1 and n2 in water buffalo. additionally, water buffa ... | 2016 | 27149894 |
| effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa. | the effects of equilibration times (e1, 2 h; e2, 4 h; e3, 6 h), freezing rates (fr1, manual, 5 cm above liquid nitrogen (ln2 ) for 10 min, plunging in ln2 ; fr2, programmable ultra-fast, holding at +4 °c for 2 min, from 4 to -10 °c at -10 °c/min, from -10 to -20 °c at -15 °c/min, from -20 to -120 °c at -60 °c/min, holding at -120 °c for 30 sec, plunging in ln2 ), and thawing rates (t1, 37 °c for 30 sec; t2, 50 °c for 15 sec; t3, 70 °c for 7 sec) were evaluated on quality of buffalo bull spermato ... | 2016 | 27153390 |
| strategies to overcome seasonal anestrus in water buffalo. | reproductive seasonality in buffalo (bubalus bubalis) is characterized by behavioral, endocrine, and reproductive changes that occur over distinct periods of the year. during the nonbreeding season (spring and summer), the greater light-dark ratio (long days) suppresses estrus behavior and the occurrence of ovulation. anestrous buffaloes have insufficient pulsatile of lh to support the final stages of follicular development, and subsequently, estrus behavior and ovulation do not occur, limiting ... | 2016 | 27157389 |
| impact of buserelin acetate or hcg administration on the day of first artificial insemination on subsequent luteal profile and conception rate in murrah buffalo (bubalus bubalis). | this study was designed to investigate the impact of buserelin acetate (ba) or human chorionic gonadotropin (hcg) administration on the day of first artificial insemination (ai) on subsequent luteal profile (diameter of corpus luteum (cl) and plasma progesterone) and conception rate in murrah buffalo. the present experiment was carried out at two locations in 117 buffalo that were oestrus-synchronized using cloprostenol (500 μg) administered (i.m.) 11 days apart followed by ai during standing oe ... | 2016 | 27170495 |
| polymorphism in the a2m gene associated with high-quality milk in murrah buffaloes (bubalus bubalis). | the study of genes associated with host defense mechanisms, such as the a2m gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. the aim of this study was to identify polymorphisms in the a2m gene in murrah buffaloes (bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. hair follicle samples of 136 animals were collected for dna extraction, and polymorphism ... | 2016 | 27173184 |
| comparative efficacy of e-17β and gnrh administration on day 0 of a controlled internal drug release (cidr) based protocol on synchrony of wave emergence, ovulation and conception rates in murrah buffalos (bubalus bubalis). | the study was undertaken to compare the efficacy of an estradiol-17β + cidr based protocol with the conventional ovsynch + cidr based protocol for synchrony of wave emergence and ovulation in murrah buffalos. in group i (n=25), on day 0 (beginning of experiment), buffaloes were administered a cidr device (1.38 g p4) and concurrently received 1.5 mg e-17β. on day 9, the cidr was removed and a prostaglandin (pg) f2α analogue (500 µg) was administered. on day 11, buffaloes were administered a gonad ... | 2015 | 27175151 |
| high genetic diversity and distribution of bubu-dqa alleles in swamp buffaloes (bubalus bubalis carabanesis): identification of new bubu-dqa loci and haplotypes. | in this study, genetic diversity analysis of mhc class ii-dqa locus helped in identification of 25 new bubu-dqa nucleotide sequences in swamp buffaloes (bubalus bubalis carabanesis, bubu). phylogenetic analysis revealed the distribution of the buffalo dqa sequences in two major clusters of dqa1 and dqa2 genes, sharing common lineages with corresponding cattle alleles, possibly due to trans-species evolution. however, a highly divergent sequence, bubu-dqa*2501, homologous to cattle (bola) dqa3 al ... | 2016 | 27177904 |
| effect of mastitis on luteal function and pregnancy rates in buffaloes. | the aim of this study was to investigate the effects of mastitis on cl development and function and pregnancy rate in buffaloes. sixty-six buffaloes (bubalus bubalus) reared in a commercial farm at el-beheira governorate, north of egypt were used in this study. according to the visual observation of milk, physical examination of the udder and actual somatic cell count in milk, buffalo cows were divided into three groups: without mastitis (w), n = 23; subclinical mastitis (sc), n = 18; and clinic ... | 2016 | 27177967 |
| sister chromatid exchange test in river buffalo lymphocytes treated in vitro with furocoumarin extracts. | furocoumarin extracts from psoralea morisiana, the endemic sardinian legume species, were tested for their mutagenic potential on river buffalo blood cells. the results obtained performing the sister chromatid exchange (sce) test in blood cultures of five river buffalo calves (exposure to furocoumarins for 72h) and five cows (exposure to furocoumarins for 3h, in the absence and presence of s9 metabolic activator) are reported. significant differences in mean values of sces were observed in cells ... | 2016 | 27180332 |
| saliva ferning, an unorthodox estrus detection method in water buffaloes (bubalus bubalis). | estrus detection is a major problem in buffalo husbandry because of inconsistent expression of estrous signs at different seasons, and a high prevalence of the silent heat and postpartum anestrus in this species. around 50% of the estrus events in buffaloes are currently undetected in the field conditions, resulting in a huge economic loss. although the cervicovaginal fluid fern patterns confirm the estrus for a breeding decision, the fluid discharge is absent during the silent-heat condition. t ... | 2016 | 27181055 |
| utility of ultrasonography for diagnosis of superficial swellings in buffalo (bubalus bubalis). | we studied 72 buffalo with superficial swellings in the head (n=4), neck (n=5), chest wall (n=4), abdominal wall (n=28), limbs (n=16), gluteal region (n=8), perineal region (n=6) and udder (n=1). ultrasonographically, the swellings varied according to type, duration, content and location. the clinical use of ultrasound to assess these superficial swellings allowed diagnosis of abscesses (n=21), hematomas (n=11), hernias (n=17), bursitis (n=13), urethral diverticula (n=6) and tumors (n=4). ultras ... | 2016 | 27181085 |
| seroprevalence of foot-and-mouth disease in susceptible wildlife in israel. | foot-and-mouth disease (fmd) epidemics recur in israel almost every year. wild even-toed ungulates are seldom affected during these epidemics. the seroprevalence of fmd in wild ungulates during 2000 and 2005-2013 was estimated using anti-non-structural proteins elisa. overall, 209 samples were tested, comprising sera of 120 wild boar (sus scrofa lybicus), 64 mountain gazelles (gazella gazella gazella), 6 water buffaloes (bubalus bubalis), and 19 persian fallow deer (dama dama mesopotamica). none ... | 2016 | 27200364 |
| comparative pharmacokinetics of ceftiofur hydrochloride and ceftiofur sodium after administration to water buffalo (bubalus bubalis). | objective to evaluate pharmacokinetics and bioavailability after administration of ceftiofur hydrochloride and ceftiofur sodium to water buffalo (bubalus bubalis). animals 5 healthy adult water buffalo (3 males and 2 nonlactating females). procedures all animals received a dose (2.2 mg/kg) of 3 ceftiofur products (2 commercially available suspensions of ceftiofur hydrochloride [cef1 and cef2, im] and ceftiofur sodium [cef3, iv]). blood samples were collected for up to 196 hours. concentrations o ... | 2016 | 27227504 |
| genetic and genomic dissection of prolactin revealed potential association with milk production traits in riverine buffalo. | milk yield and quality has been a major selection criterion for genetic improvement in livestock species. role of prolactin gene in determining milk quality in terms of protein profile, lactose, lipids and other imperative macromolecules is very important. in this context, genetic profiling of prolactin gene in riverine buffalo of pakistan was performed and potential genetic markers were identified illustrating worth of this gene in marker-assisted selection of superior dairy buffaloes. series o ... | 2016 | 27240674 |
| use of peripheral blood for production of buffalo (bubalus bubalis) embryos by handmade cloning. | buffalo embryos were produced by handmade cloning using peripheral blood-derived lymphocytes as donor cells. although the blastocyst rate was lower (p < 0.01) for lymphocyte- than control skin fibroblast-derived embryos (6.6 ± 0.84% vs. 31.15 ± 2.97%), the total cell number (152.6 ± 23.06 vs. 160.1 ± 13.25) and apoptotic index (6.54 ± 0.95 vs. 8.45 ± 1.32) were similar. the global level of h3k9ac was higher (p < 0.05) in lymphocyte- than that in skin-derived blastocysts; whereas in ivf blastocys ... | 2016 | 27242179 |