Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| chlamydia psittaci, helicobacter pylori and ocular adnexal lymphoma-is there an association? the german experience. | 2007 | 17257672 | |
| a comparative study of detecting chlamydophila psittaci in pet birds using isolation in embryonated egg and polymerase chain reaction. | this study, for the first time in turkey, investigated the existence of chlamydophila psittaci and determined the prevalence of its disease, chlamydiosis, in pet birds. polymerase chain reaction (pcr) was compared with other testing methods that have been typically used in the diagnosis of c. psittaci. fecal specimens (n =96) of avian origin were tested by pcr and two identification methods, modified gimenez staining (mgs) and direct fluorescein-conjugated monoclonal antibody staining (fa). the ... | 2006 | 17274283 |
| multiple sites of exposure in an outbreak of ornithosis in workers at a poultry abattoir and farm. | cases of ornithosis amongst workers on a rural duck abattoir and farm were notified from late 2003 to health authorities in victoria, australia. in may 2004 we conducted a serological survey to identify the extent of exposure to chlamydophila psittaci amongst workers and a case control study to identify high-risk work areas for ornithosis-related pneumonia. some workers in all occupational groups showed serological evidence of exposure, while those with pneumonia were more likely to have worked ... | 2007 | 17274860 |
| health evaluation of free-ranging and captive blue-fronted amazon parrots (amazona aestiva) in the gran chaco, bolivia. | bolivia has a total of 47 species of psittacidae, seven of which have been identified in our study site, the semiarid gran chaco of the isoso. one species, the blue-fronted parrot (amazona aestiva), is frequently captured by local isoseño guaraní indians for exploitation on the national and international market. these birds are often temporarily housed in small villages under unhygienic conditions with poultry and other domestic species. on occasion, these parrots escape back to the wild. additi ... | 2005 | 17312715 |
| low prevalence of chlamydia psittaci in ocular adnexal lymphomas from cuban patients. | most ocular adnexal lymphomas (oal) are extranodal marginal zone b-cell lymphomas (emzl) of mucosa-associated lymphoid tissue (malt)-type. chronic antigen stimulation has been suggested to have a pathogenetic role in emzl and chlamydia psittaci chronic infection has been recently associated with the development of oal in a series of patients from italy. to assess this association, an evaluation of the presence of c. psittaci was made in a different oal population. dna samples were obtained from ... | 2007 | 17325853 |
| genotyping of chlamydophila psittaci in human samples. | chlamydophila (formerly chlamydia) psittaci genotypes a, b, c, and a new genotype most similar to the 6bc type strain were found in 10 humans with psittacosis by outer membrane protein a gene sequencing. genotypes b (n = 3) and c (n = 1) are endemic in nonpsittacine european birds. these birds may represent an important part of the zoonotic reservoir. | 2006 | 17326961 |
| [the case of 57-year-old patient with ornithosis]. | the authors describe the case of ornithosis in the form of severe pleuropneumonia with breathing disorders. they emphasize that the proper diagnosis and consequently the treatment have been achieved by the detailed anamnesis and not by additional tests, as the immune reaction was found after full remission of pathological lesions. | 2006 | 17338137 |
| ten years of viral and non-bacterial serology in adults with cystic fibrosis. | viral infections are associated with pulmonary exacerbations in children with cystic fibrosis (cf), but few studies have addressed the frequency in adults. this paper investigates the frequency and impact of viral infections in adults with cf receiving intravenous antibiotics. pre- and post-treatment spirometry, inflammatory markers and antibody titres against influenza a, influenza b, adenovirus, respiratory syncytial virus, mycoplasma pneumoniae, chlamydia psittaci, and coxiella burnetti were ... | 2008 | 17352838 |
| evaluation of two commercial assays for the detection of chlamydophila abortus antibodies. | two commercial enzyme-linked immunosorbent assays (elisa), the chekit-chlamydia which uses inactivated chlamydophila psittaci antigen, and the chlamydophila abortus elisa produced by the institut pourquier which uses a recombinant fragment of the 80-90 kda protein, were evaluated with the objective to determine whether the new elisas would perform as improved alternatives to the complement fixation test (cft) for the serological diagnosis of ovine enzootic abortion (oea). the results were compar ... | 2007 | 17382493 |
| immune-mediated hemolytic anemia in an eclectus parrot. | a 2-year-old female solomon island eclectus parrot (eclectus roratus) was evaluated by a veterinarian because of a 4-day history of progressive lethargy, weakness, poor appetite, and inactivity. the bird was referred to a veterinary teaching hospital for further examination. | 2007 | 17397343 |
| [two familial cases of psittacosis]. | we report here 2 cases of psittacosis in a family. in the first case, a 51-year-old woman was admitted with fever, dry cough, and a chest radiograph showed increased opacity in the right upper lung field. on a diagnosis of atypical pneumonia, minocycline was given and her clinical symptoms and abnormal laboratory data were improved. the second case was her husband, a 58-year-old man who presented with fever 4 days after his wife's admission. his chest radiograph revealed increased opacity in the ... | 2007 | 17419438 |
| lack of an association between chlamydia psittaci and ocular adnexal lymphoma. | the objective of this study was to assess whether there is pcr evidence for c. psittaci dna in ocular adnexal lymphoma specimens collected in an academic institution in the u.s. this was a retrospective, single-center study of patients from 1994 - 2004. we used 28 ocular adnexal lymphoma biopsy specimens from adult patients, 16 control lymphoma specimens from patients with systemic lymphomas not involving the ocular adnexa, and five control benign adnexal tissue samples. the presence of c. psitt ... | 2007 | 17454602 |
| detection of borrelia burgdorferi sensu lato and chlamydophila psittaci in throat and cloacal swabs from birds migrating through slovakia. | we have screened 91 migratory birds representing 32 species during the autumn of 2003 for the presence of the zoonotic pathogens borrelia and chlamydophila. using polymerase chain reaction (pcr), b. burgdorferi sensu stricto was detected in cloacal swabs and, in two causes, also in throat swabs in 8 individuals (8.7 %) representing 7 birds species; b. garinii and b. afzelii were not detected. c. psittaci was detected only in cloacal swabs; 6 birds (6.6 %) from four species were found to be posit ... | 2006 | 17455806 |
| extranodal marginal zone b-cell lymphomas of the ocular adnexa: multiparameter analysis of 34 cases including interphase molecular cytogenetics and pcr for chlamydia psittaci. | extranodal marginal zone b-cell lymphomas of malt type (malt lymphomas) show site-dependent variations in their morphologic, phenotypic, and/or cytogenetic findings. this report describes a comprehensive analysis of 34 ocular adnexa malt lymphomas, including interphase fluorescence in situ hybridization for malt lymphoma-associated cytogenetic abnormalities and polymerase chain reaction for chlamydia psittaci, which has recently been suggested to be associated with ocular adnexa lymphomas. a typ ... | 2007 | 17460465 |
| chlamydiae and atherosclerosis: can psittacine cases support the link? | atherosclerosis is a common disease in pet birds, particularly in psittacines. little is known about the role of risk factors predisposing birds to this disease. in our study, we tried to detect chlamydiae in formalin-fixed and paraffin-embedded atherosclerotic tissue from 103 pet birds to clarify their role in atherosclerosis. methods used were polymerase chain reaction (pcr), sequencing, and immunohistochemistry. histopathologic examination served to classify the extent of atherosclerotic lesi ... | 2007 | 17461260 |
| detection of helicobacter pylori and chlamydia pneumoniae genes in primary orbital lymphoma. | primary orbital non-hodgkin's lymphoma is a mucosa-associated lymphoid tissue (malt)-type extranodal marginal zone lymphoma. chronic antigen stimulation is implicated as a causative agent in the development of some mature b-cell proliferations; for example, there are associations involving helicobacter pylori with gastric or conjunctival malt lymphoma and chlamydia psittaci with ocular adnexal lymphoma. we examined the molecular signatures of h pylori and chlamydia in eight orbital lymphomas. | 2006 | 17471326 |
| absence of chlamydial infection in japanese patients with ocular adnexal lymphoma of mucosa-associated lymphoid tissue. | ocular adnexal extranodal marginal zone b-cell lymphomas of mucosa-associated lymphoid tissue (ocular adnexal malt lymphoma) are predominately low-grade, small b-cell types and may be caused by several putative inflammatory agents. twenty-three ocular adnexal malt lymphoma cases, the monoclonality of which was confirmed by examination of immunoglobulin heavy chain gene rearrangement and/or cell surface antigens, were analyzed for evidence of several causative factors. a serologic evaluation of o ... | 2007 | 17483059 |
| [detection of some zoonotic agents in the domestic pigeon (columba livia) in the city of chillán, chile]. | there is an increase in the population of doves (columba livia) as well as their contact with healthy and immunocompromised hosts. | 2007 | 17554438 |
| an outbreak of psittacosis in a bird park in japan. | an outbreak of psittacosis related to a bird park occurred in matsue city, shimane prefecture, japan, during winter 2001. seventeen cases of psittacosis (12 visitors, three staff, and two student interns) were confirmed. a cohort study was conducted among the park staff and students to determine the risk factors for the development of acute serologically confirmed psittacosis (scp) infection. being 'bird staff' had an increased risk of scp infection (rr 3.96, 95% ci 1.48-10.58). entering the sta ... | 2008 | 17559693 |
| chlamydia psittaci infection and clinicopathologic analysis of ocular adnexal lymphomas in korea. | ocular adnexal lymphoma (oal) is a mostly extranodal marginal zone lymphoma (emzl). recent findings have suggested an association between chlamydia psittaci (cp) infection and oal. we sought to confirm this issue and to analyze the clinicopathologic characteristics of oal in korea. between 1993 and 2004, 33 oal cases were identified at the asan medical center, seoul, korea. dna was extracted from paraffin-embedded tissues, and touchdown enzyme time release polymerase chain reaction was performed ... | 2007 | 17570512 |
| marginal-zone lymphoma. | the term marginal-zone lymphoma (mzl) encompasses three closely related lymphoma subtypes, namely the "low-grade b-cell lymphoma of malt type" currently named malt lymphoma, the "nodal marginal-zone b-cell lymphoma" and a provisional entity in the real classification named "primary splenic mzl with or without villous lymphocytes". these entities display different characteristics, with evident clinical and biological variations according to the organ where the lymphoma arises. marginal-zone b-cel ... | 2007 | 17583528 |
| meta-analyses of the association between chlamydia psittaci and ocular adnexal lymphoma and the response of ocular adnexal lymphoma to antibiotics. | there are conflicting reports regarding the association between chlamydia psittaci (cps) and ocular adnexal lymphoma (oal) and the efficacy of antibiotics for oal. in the current study, the authors attempted to clarify the association between cps and oal and the efficacy of antibiotics for oal. | 2007 | 17594698 |
| reovirus infections associated with high mortality in psittaciformes in the netherlands. | in the netherlands between january 2002 and december 2004, numerous psittaciformes died showing severe splenomegaly and hepatomegaly with multifocal acute necrosis. at the start of the outbreaks mostly parakeets were affected, but later larger parrots were also involved. seventy-eight birds showed the same features and six were examined completely, including a virological examination. tests for polyomavirus, pacheco's disease (herpesvirus) and circovirus psittacine beak and feather disease (pbfd ... | 2007 | 17620175 |
| effect of ovotransferrin and lactoferrins on chlamydophila psittaci adhesion and invasion in hd11 chicken macrophages. | the effect of ovotransferrin (ovotf), human lactoferrin (hlf) and bovine lactoferrin (blf) on the obligate intracellular pathogen chlamydophila (cp.) psittaci was evaluated using a model of buffalo green monkey kidney (bgm) cells and hd11 chicken macrophages as artificial hosts. firstly, the effect of transferrins on the infectivity of the bacteria was evaluated. pre-incubation of cp. psittaci with 0.5 to 5 mg/ml ovotf prior to infecting bgm cells significantly lowered the infection rate (p < 0. ... | 2007 | 17621441 |
| investigation on the agonistic and antagonistic biological activities of synthetic chlamydia lipid a and its use in in vitro enzymatic assays. | the synthetic 1,4'-bisphosphorylated penta-acyl and tetra-acyl lipid a structures representing the major molecular species of natural chlamydial lipid a were tested for their endotoxic activities as measured by interleukin-8 release from human embryonic kidney (hek) 293 cells expressing toll-like receptor (tlr) 2 or tlr4. both compounds were unable to activate hek293 cells transiently transfected with tlr2. the penta-acyl lipid a was a weak activator of hek293 cells expressing tlr4/md-2/cd14 whe ... | 2007 | 17621554 |
| q fever community-acquired pneumonia in a patient with crohn's disease on immunosuppressive therapy. | community-acquired pneumonia (cap) may be caused by typical or atypical pathogens. the three most common zoonotic atypical pathogens are chlamydophila psittaci (psittacosis), francisella tularensis (tularemia), and coxiella burnetii (q fever). atypical caps are suggested by a distinctive pattern of extrapulmonary organ involvement. zoonotic cap may be differentiated from nonzoonotic cap (chlamydia pneumoniae, mycoplasma pneumoniae, legionnaire's disease) by a recent zoonotic vector contact histo ... | 2007 | 17628200 |
| construction and immunogenicity of recombinant adenovirus expressing the major outer membrane protein (momp) of chlamydophila psittaci in chicks. | avian chlamydiosis is caused by chlamydophila psittaci. the major outer membrane protein (momp) encoded by the outer membrane protein 1 (omp1) gene is an excellent candidate for genetic engineering of a vaccine against avian chlamydiosis. in this study, the momp gene was amplified by pcr and cloned into the transfer vector pshuttle-cmv. the recombinant plasmid was obtained by recombination between the plasmid pshuttle-cmv-momp and skeleton vector padeasy-1 in escherichia coli strain bj5183. the ... | 2007 | 17640776 |
| the p47 gtpases iigp2 and irgb10 regulate innate immunity and inflammation to murine chlamydia psittaci infection. | c57bl/6j mice were 10(5)-fold more resistant to chlamydia psittaci infection than dba/2j mice by ld(100) determinations. linkage analysis using bxd recombinant inbred strains revealed a single effector locus at a 1.5-mbp region on chromosome 11 encoding a cluster of three p47 gtpases (irgb10, igtp, and iigp2). western blots of infected tissue showed that irgb10 was elevated in resistant mice and one of the two possible iigp2 protein isoforms was preferentially expressed in susceptible mice. the ... | 2007 | 17641048 |
| chlamydophila psittaci genotype e/b transmission from african grey parrots to humans. | thirty-six birds from a parrot relief and breeding centre, as well as the manager, were examined for the presence of chlamydophila psittaci. in the relief unit, 5 of 20 african grey parrots showed depression, ruffled feathers, loss of weight and mild dyspnoea. the birds received no antibiotic treatment. birds of the breeding unit, 14 blue and gold macaws and 2 green-winged macaws, were healthy. they received doxycycline at the start of each breeding season. the manager complained of shortness of ... | 2007 | 17644718 |
| serologic investigation of the prevalence of chlamydophila psittaci in occupationally-exposed subjects in eastern sicily. | the aim of the study was to investigate the occurrence of serum antibodies to c. psittaci in workers at 8 stock farms located in a rural area of eastern sicily. serum samples from 188 workers and 160 controls were tested for immunoglobulin iga, igg and igm direct against c. psittaci by microimmunofluorescent test (mif). seropositive subjects were defined as those with igg titres of 1:16-1:256, and seronegative subjects as those whose titre was <1:16. to rule out the presence of cross reactive an ... | 2007 | 17655184 |
| detection of chlamydophila psittaci antibodies from captive birds at the ninoy aquino parks and wildlife nature center, quezon city, philippines. | the present study was undertaken to detect the presence of chlamydophila psittaci antibodies in captive birds at the wildlife rescue center, ninoy aquino parks and wildlife nature center, quezon city, philippines. blood was collected from 36 birds of different species and the presence of antibodies against c. psittaci was detected using an elisa-based test kit. 25% of the samples demonstrated antibodies against c. psittaci. the results of this study confirmed the presence of c. psittaci antibodi ... | 2007 | 17655198 |
| [sporadic forms of ornithosis (psittacosis) in a clinician's practice]. | for a long time ornithosis has been considered an occupational disease, while the significance of sporadic (home) forms of the illness has been underestimated due to difficult clinical diagnostics which results from polymorphic clinical manifestations and the absence of appropriate epidemiological data in home cases of the disease. sporadic ornithosis (psittacosis) is a pathology which is spread widely enough and whose diagnosis is very difficult. one of the most typical manifestations is pulmon ... | 2007 | 17665610 |
| lymphoproliferative disease of the orbit. | malignant lymphoma of the ocular adnexa has been reported on for many years, but many steps forward have been recently made. this paper highlights the staging strategies and treatment options based on a review of the most updated and relevant bibliography. | 2007 | 17700233 |
| chlamydophila psittaci dna detection in the faeces of cage birds. | in this study, we investigated the shedding of chlamydophila psittaci in faecal samples from cage birds using pcr testing. a total of 47 faeces samples were collected from four different aviaries. main symptoms determined after clinical investigation and owner histories of the birds showed that the birds had respiratory system problems changing from mild to severe. they also showed conjunctivitis, diarrhoea or no symptoms at all. dna extractions from faeces were performed with the qiaamp dna sto ... | 2007 | 17803512 |
| a woman and her canary: a tale of chlamydiae and lymphomas. | 2007 | 17848672 | |
| microbiological aspects of bacterial lower respiratory tract illness in children: atypical pathogens. | 'atypical' lower respiratory tract pathogens often cause a distinct identifiable syndrome in adults, but in children the clinical presentation of atypical, typical and viral pneumonia is less well differentiated. specific microbiological investigations are usually required, but an understanding of their strengths and weaknesses is necessary to make interpretation possible. this review examines clinical presentation, microbiology and current evidence surrounding diagnostic techniques for mycoplas ... | 2007 | 17868919 |
| chlamydiae and mycoplasma infections in pulmonary malt lymphoma. | chlamydia pneumoniae, chlamydia trachomatis and chlamydia psittaci were detected at low frequencies (<20%) among 69 pulmonary mucosa-associated lymphoid tissue (malt) lymphomas, 30 other lymphoproliferative disorders (lpd) and 44 non-lpd. the incidence of individual chlamydiae was generally higher in malt lymphoma than non-lpd, although not reaching statistical significance. mycoplasma pneumoniae dna was not detected. | 2007 | 17876330 |
| frequency of development and associated physiological cost of azithromycin resistance in chlamydia psittaci 6bc and c. trachomatis l2. | azithromycin is a major drug used in the treatment and prophylaxis of chlamydial infections. spontaneous azithromycin-resistant mutants of chlamydia psittaci 6bc were isolated in vitro in the plaque assay at a frequency of about 10(-8). isogenic clonal variants with a(2058)c, a(2059)g, or a(2059)c mutations in the unique 23s rrna gene (escherichia coli numbering system) displayed mics for multiple macrolides (i.e., azithromycin, erythromycin, josamycin, and spiramycin) at least 100 times higher ... | 2007 | 17908942 |
| infectious disease associations in advanced stage, indolent lymphoma (follicular and nonfollicular): developing a lymphoma prevention strategy. | eradication of helicobacter pylori in gastric mucosa-associated lymphoid tumor can result in lymphoma remission. we prospectively identified/treated infections in nonbulky, advanced stage indolent lymphoma (follicular; nonfollicular lymphoma) eligible for observation. materials and methods: stool h. pylori, hepatitis c and borrelia serologies, borrelia and chlamydia fixed tissue pcr, chlamydia peripheral blood mononuclear cell pcr and hydrogen breath test for small bowel bacterial overgrowth (sb ... | 2008 | 17965114 |
| ocular adnexal malt lymphoma: an intriguing model for antigen-driven lymphomagenesis and microbial-targeted therapy. | non-hodgkin's lymphomas constitute one half of malignancies arising in the orbit and the ocular adnexae. mucosa-associated lymphoid tissue (malt)-type lymphoma is the most common histological category in this anatomic region. the incidence of ocular adnexal lymphoma of mucosa-associated lymphoid tissue-type (oaml) is increasing and recent studies offered new relevant insights in molecular, pathogenetic and therapeutic issues on these neoplasms. a pathogenetic model of antigen-driven lymphoprolif ... | 2008 | 17986622 |
| evaluation of a chlamydophila psittaci infection diagnostic platform for zoonotic risk assessment. | reports on zoonotic transmission of chlamydophila psittaci originating from poultry are incidentally published. during recent studies in european turkeys we isolated c. psittaci genotypes a, b, d, e, f, and e/b, all considered potentially dangerous for humans. this encouraged us to analyze the zoonotic risk on a belgian turkey farm, from production onset until slaughter, using a chlamydophila psittaci diagnostic platform. twenty individually marked hens, as well as the farmer and two scientists, ... | 2008 | 18003799 |
| malt lymphoma : recent advances in aetiology and molecular genetics. | mucosa-associated lymphoid tissue (malt) lymphoma is a common low grade b-cell lymphoma arising from a background of chronic inflammatory disease at a number of mucosal sites. those originating in the stomach are causatively linked to helicobacter pylori infection and eradication of the bacterium with antibiotics leads to long-term complete regression of the lymphoma in aproximately 70% of cases. now, there is further evidence of linking campylobacter jejuni, borrelia burgdorferi and chlamydia p ... | 2007 | 18040143 |
| a dutch case of atypical pneumonia after culling of h5n1 positive ducks in bavaria was found infected with chlamydophila psittaci. | 2007 | 18053568 | |
| low potency of chlamydophila lps to activate human mononuclear cells due to its reduced affinities for cd14 and lps-binding protein. | chlamydiaceae are small obligate intracellular parasites and classified as gram-negative bacteria. among chlamydiaceae-derived components, lps is known as an immunomodulator and possesses a unique lipid a structure with longer but fewer acyl chains. in this study, to elucidate the chlamydiaceae-induced immune responses, we evaluated the actions of chlamydophila psittaci lps as a chlamydiaceae lps on human pbmcs and compared with those of escherichia coli lps. similar to e. coli lps, c. psittaci ... | 2008 | 18056918 |
| serological profiling with chlamycheck, a commercial multiplex recombinant antigen western blot assay of chlamydial infections. | a new chlamydial test system, the chlamycheck assay, which uses 4 purified recombinant antigens of chlamydia trachomatis and chlamydophila pneumoniae and one antigen of chlamydophila psittaci, has been developed and commercialized. we investigated the reactivities of the recombinant antigens with sera from a group of 30 patients with acute chlamydia trachomatis infection, 88 patients consulting for sexually transmitted infections, and 46 patients with serological evidence of chlamydophila pneumo ... | 2007 | 18059568 |
| chlamydia psittaci in malt lymphomas of ocular adnexals: the austrian experience. | the presence of chlamydia (c.) psittaci, c. pneumoniae and c. trachomatis dnas in malt lymphomas of ocular adnexals from 13 austrian patients were studied. gastrointestinal malt lymphomas and gastritis specimens served as controls. of 13 malt lymphomas of the ocular adnexals, seven were positive for c. psittaci dna. in contrast, one of 17 gastrointestinal specimens tested positive. all specimens were negative for c. trachomatis and c. pneumoniae dnas. in conclusion, c. psittaci infection was obs ... | 2008 | 18061259 |
| chlamydia-psittaci-eradicating antibiotic therapy in patients with advanced-stage ocular adnexal malt lymphoma. | 2008 | 18073219 | |
| local cluster of psittacosis after bird show in the netherlands, november 2007. | 2007 | 18082112 | |
| [a contribution to the nomenclature and classification of chlamydiae]. | the results of molecular-genetic study of typical specimens of the genus chlamydophila of the family chlamydiaceae from the fse fctrsa collection of microorganisms are considered for the purpose of their taxonomy based on comparative analysis by the omp1-, omp2-, 16s rrna-, and 23s rrna-genes and by the presence of extrachromosomal plasmid with corresponding fragments of the genomes officially registered species of chlamydiae. according to the omp1-rflp-alui-profile, the rostinovo-70, 250, pp-87 ... | 2007 | 18154077 |
| multiple chlamydiaceae species in trachoma: implications for disease pathogenesis and control. | chlamydia trachomatis is a unique obligate intracellular bacterium that remains the leading cause of sexually transmitted bacterial diseases and preventable blindness worldwide. chronic ocular infections are referred to as trachoma, and predominate in developing countries. since 2001, the world health organization has promoted control strategies including antibiotics, improved hygiene, and environmental measures with limited success. consequently, a vaccine is urgently needed. integral to vaccin ... | 2008 | 18177205 |
| real-time pcr detection and quantitation of chlamydophila psittaci in human and avian specimens from a veterinary clinic cluster. | we report three cases of psittacosis in staff working in a veterinary surgery, which was related to exposure to a sick, wild psittacine bird. chlamydial genus- and chlamydial species-specific dna was detected in clinical specimens, including throat swabs, whole blood and urine. the organism load was quantified by real-time pcr (rt-pcr), which revealed 10(5)-fold more organisms in conjunctival swabs from the source bird than in the human samples. one clinic attendant was infected despite using pe ... | 2008 | 18183441 |
| [molecular genetic characterization of avian chlamydophila psittaci isolates]. | restriction enzyme analysis of the omp1 gene was used to characterize 51 avian chlamydophila psittaci isolates. the analysis confirmed the predominance of genotype a in parrot c. psittaci isolates and revealed new omp1 genotypes in corvid c. psittaci isolates. the corvid isolates proved to lack an extrachromosomal plasmid. the omp1 and rrna igs sequences were determined for the new isolates. phylogenetic analysis showed that isolate 1v, obtained from a crow, is intermediate in several characters ... | 2007 | 18186183 |
| emerging role of chlamydia and chlamydia-like organisms in adverse pregnancy outcomes. | this review considers the roles of chlamydia spp. and newly identified chlamydia-like organisms in miscarriage, stillbirths and preterm labour in both animals and humans. | 2008 | 18192789 |
| chlamydophila psittaci transmission from pet birds to humans. | we studied zoonotic transmission of chlamydophila psittaci in 39 breeding facilities for psittaciformes (cockatoos, parrots, parakeets, lories) that frequently used antimicrobial drugs. genotypes a or e/b were detected in 14.9% of humans at these facilities. information on antimicrobial drug use in psittaciformes and a c. psittaci vaccine are urgently required. | 2007 | 18214194 |
| chlamydophila spp. infection in horses with recurrent airway obstruction: similarities to human chronic obstructive disease. | recurrent airway obstruction (rao) in horses is a naturally occurring dust-induced disease mainly characterized by bronchiolitis which shows histological and pathophysiological similarities to human chronic obstructive pulmonary disease (copd). in human copd previous investigations indicated an association with chlamydophila psittaci infection. the present study was designed (1) to clarify a possible role of this infectious agent in rao and (2) to investigate the suitability of this equine disor ... | 2008 | 18230187 |
| high resolution typing of chlamydophila psittaci by multilocus vntr analysis (mlva). | a multilocus vntr analysis (mlva) system for detection of tandem repeats across the whole genome of chlamydophila psittaci has been developed. twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (a to f). thereafter, 8 loci were retained for a more complete study performed on over 150 c. psittaci isolates from different bird species and geographical origins. comparative analysis of the mlva results and those obtained fr ... | 2008 | 18242149 |
| exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes. | to explore the molecular basis of antigen recognition by germline antibodies, we have determined to high resolution the structures of the near-germline monoclonal antibody s25-2 in complex with seven distinct carbohydrate antigens based on the bacterial sugar 3-deoxy-alpha-d-manno-oct-2-ulosonic acid (kdo). in contrast to previous findings, the inherited germline kdo monosaccharide binding site is not restricted to this bacterial sugar but is able to accommodate an array of substitutions and che ... | 2008 | 18272175 |
| identification and characterization of a type iii secretion system in chlamydophila psittaci. | chlamydiaceae are obligate intracellular gram-negative bacteria replicating in vacuoles inside eukaryotic cells. it has been proven that most of them possess a type iii secretion system (t3ss) allowing them to transfer effector molecules in the host cell. we examined the existence of a t3ss in chlamydophila psittaci by studying the expression of three essential structural proteins sctw, sctc, and sctn, and one putative effector protein inca. immunofluorescence assays showed sctw and inca to be a ... | 2008 | 18275803 |
| [primary ocular adnexal malt lymphoma]. | 2008 | 18277591 | |
| iron depletion limits intracellular bacterial growth in macrophages. | many intracellular pathogens infect macrophages and these pathogens require iron for growth. here we demonstrate in vitro that the intracellular growth of chlamydia psittaci, trachomatis, and legionella pneumophila is regulated by the levels of intracellular iron. macrophages that express cell surface ferroportin, the only known cellular iron exporter, limit the intracellular growth of these bacteria. hepcidin is an antimicrobial peptide secreted by the liver in response to inflammation. hepcidi ... | 2008 | 18369153 |
| chlamydophila psittaci infections in humans during an outbreak of psittacosis from poultry in germany. | in 2005, an outbreak of severe respiratory disease in a mixed poultry flock that was infected with chlamydophila (c.) psittaci led to dissemination of the infection to at least 100 small poultry farms in 11 districts of central germany. at the same time, a total of 24 persons in contact with poultry from one of the flocks reported flu-like symptoms to their physician, thus suggesting zoonotic transmission. within 3 weeks, seven individuals had to be hospitalized, with three of them requiring int ... | 2008 | 18387139 |
| new real-time pcr tests for species-specific detection of chlamydophila psittaci and chlamydophila abortus from tissue samples. | chlamydophila psittaci and chlamydophila abortus are the causative agents of avian chlamydiosis (psittacosis) and ovine enzootic abortion, respectively. both pathogens are known to possess zoonotic potential. due to their close genetic relatedness, direct and rapid species identification is difficult. in the present study, new real-time pcr assays are reported for both species. the tests are based on highly specific probes targeting the ompa gene region and were conducted as duplex pcrs includin ... | 2009 | 18413292 |
| genotyping of chlamydophila psittaci using a new dna microarray assay based on sequence analysis of ompa genes. | the currently used genotyping system for the avian zoonotic pathogen chlamydophila (c.) psittaci has evolved from serology and is based on ompa sequence variations. it includes seven avian and two non-avian genotypes. restriction enzyme cleavage of the amplified ompa gene and, less frequently, ompa sequencing are being used for examination, but, beside methodological limitations, an increasing number of recently tested strains could not be assigned to any established genotype. | 2008 | 18419800 |
| isolation and characterization of chlamydophila psittaci isolated from laying hens with cystic oviducts. | the objective of this study was to isolate and identify a hypothetical chlamydiaceae pathogen from laying hens with an oviduct cyst, and to characterize its potential causal relation with decreased egg production. our clinical survey showed that cystic oviducts were prevalent at rates of 10% and 15.1% in breeder and commercial hen flocks, respectively. chlamydial antigens were detected in 20 of 50 pharyngeal swabs (40%) and in 17 of 20 oviduct tissues (85%) using enzyme-linked immunosorbent assa ... | 2008 | 18459300 |
| health evaluation of free-ranging humboldt penguins (spheniscus humboldti) in peru. | as part of ongoing ecological studies of humboldt penguins (spheniscus humboldti) at punta san juan, ica department, peru, health surveys were conducted in november 1992, 1993, and 1994. in the three surveys, 98 birds in total were handled for examination, and blood was collected for laboratory analysis from 90 of these birds. all birds seemed to be in good condition. body weights of females were significantly lower in 1994 than in the other years. fleas (parapsyllus humboldti) and ticks (ornith ... | 2008 | 18459309 |
| ocular adnexal lymphoma: no evidence for bacterial dna associated with lymphoma pathogenesis. | ocular adnexal lymphomas (oals) are the most common tumors of the eye, the majority being extranodal mucosa-associated lymphoid tissue (malt) lymphomas. association with chlamydia psittaci was described in some geographic areas. oal response to antibiotic therapy was reported in cases not harboring chlamydia psittaci dna, suggesting that other bacterial infection might be implicated in the pathogenesis. we examined 49 malt oals for bacterial dna using two distinct polymerase chain reaction techn ... | 2008 | 18492114 |
| chlamydophila psittaci is viable and infectious in the conjunctiva and peripheral blood of patients with ocular adnexal lymphoma: results of a single-center prospective case-control study. | ocular adnexal malt lymphoma (oaml) is linked to chlamydophila psittaci (cp) infection. viability and infectivity of cp, demonstrated by growth in culture, has not been yet investigated in these patients. we conducted a single-center prospective case-control study to assess the prevalence, viability and infectivity of cp in 20 oaml patients and 42 blood donors registered in a 6-month period. the presence of cp in conjunctival swabs and peripheral blood mononuclear cells (pbmc) of patients and do ... | 2008 | 18528860 |
| local newspaper as a diagnostic aid for psittacosis: a case report. | 2008 | 18540809 | |
| the role of infectious agents in the etiology of ocular adnexal neoplasia. | given the fact that infectious agents contribute to around 18% of human cancers worldwide, it would seem prudent to explore their role in neoplasms of the ocular adnexa: primary malignancies of the conjunctiva, lacrimal glands, eyelids, and orbit. by elucidating the mechanisms by which infectious agents contribute to oncogenesis, the management, treatment, and prevention of these neoplasms may one day parallel what is already in place for cancers such as cervical cancer, hepatocellular carcinoma ... | 2008 | 18572051 |
| expression of chlamydophila psittaci momp heat-labile toxin b subunit fusion gene in transgenic rice. | a dna fragment encoding the momp gene of chlamydophila psittaci was fused to the heat-labile toxin b subunit gene (ltb-momp) and transferred into rice callus by agrobacterium tumefaciens-mediated transformation. the ltb-momp fusion gene was detected in genomic dna from transformed rice leaves by southern blot and rt-pcr amplification. synthesis and assembly of the ltb-momp fusion protein into pentamers was detected in transformed leaf extracts by immunoblot analysis. binding of the pentamers to ... | 2008 | 18579410 |
| the detection of chlamydophila psittaci genotype c infection in dogs. | reports of canine chlamydiosis are infrequent, possibly because the pathogen is rarely considered to be a cause of disease in dogs. this report presents details of chlamydophila psittaci infection in four bitches with recurrent keratoconjunctivitis, severe respiratory distress and reduced litter size (up to 50% stillborn or non-viable puppies) in a small dog-breeding facility in germany. cell culture and immunofluorescence examination of conjunctival, nasal and pharyngeal swabs revealed chlamydi ... | 2009 | 18583165 |
| treatment of mucosa-associated lymphoid tissue-type ocular adnexal lymphoma. | new data have recently been reported in ocular adnexal lymphomas (oals), particularly of mucosa-associated lymphoid tissue (malt)-type, regarding their biological characteristics and therapeutic management. a possible association between oal and chlamydia psittaci infection has suggested new mechanisms of lymphomagenesis and opened the way for specific targeted treatment. similarly, the place of rituximab monoclonal antibody therapy and more conventional chemotherapy, as well as a 'wait-and-see' ... | 2008 | 18594208 |
| evaluation of the prophylactic use of ovotransferrin against chlamydiosis in spf turkeys. | chlamydophila (c.) psittaci infections are highly prevalent in turkeys and the economical and public health importance of these infections has been recognized since 1950. as there are no vaccines, antibiotic treatment (tetracylines, enrofloxacine) is often needed to allow marketing of poultry. in this study, we explored the use of ovotransferrin (ovotf), a natural anti-microbial protein, in preventing an experimental c. psittaci infection in specific pathogen free (spf) turkeys. turkeys were tre ... | 2008 | 18603383 |
| tetracyclines-extending the atypical spectrum. | the main features and the present position of tetracyclines are reviewed. the mechanism of their action, bacterial resistance and the most recent findings are reported. their decreased use is due to the availability of new, active, better-tolerated antibiotics. however, tetracyclines still have a place in the treatment of chlamydial and rickettsial infections, brucellosis and lyme disease. in respiratory infections, they can be employed when necessary in infections caused by chlamydia psittaci, ... | 1993 | 18611577 |
| ultrastructural studies of chlamydia-infected air sacs of chicks. | the air sac of chicks inoculated with chlamydia psittaci was examined ultrastructurally. the epithelial cells had minute chlamydial inclusion bodies consisting of a few reticulate bodies in the initial stages of infection. in the epithelial cells in the early and middle stage of infection, small- to medium-sized chlamydial inclusion bodies were found in the cytoplasm adjacent to the golgi apparatus and surrounded with many mitochondria. the cytoplasm having chlamydial inclusion bodies was rich i ... | 1992 | 18670959 |
| pathogenic staphylococci and staphylococcal infections in canaries. | staphylococcus aureus was isolated from six cases of bacterial infection in canaries, staphylococcus hyicus from three and staphylococcus intermedius from one case. staphylococcus aureus was most often involved in outbreaks of septicaemia, with or without 'megabacterium' proventriculitis. the other coccal species were mainly complicating disease caused by atoxoplasma (lankesterella), chlamydia psittaci and canary pox. | 1994 | 18671080 |
| pathogenicity for turkeys of chlamydia psittaci strains belonging to the avian serovars a, b and d. | four groups of 20 specific pathogen-free turkeys kept in isolators were exposed to aerosols of one of four chlamydia psittaci strains. these were strain 92/1293 (avian serovar d), strain 84/55 (avian serovar a), the texas turkey strain (avian serovar d) and strain 89/1326 (avian serovar b). a further group of four specific pathogen-free turkeys consisted of sham-infected controls. the birds were observed daily for clinical signs. at daily intervals for 10 days and then twice weekly up to 34 days ... | 1994 | 18671090 |
| variable association between chlamydophila psittaci infection and ocular adnexal lymphomas: methodological biases or true geographical variations? | since the first publication in 2004, a large number of reports have raised the question of an association between ocular adnexal lymphoma and chlamydia psittaci. the results of this scientific debate, however, remain controversial. the primary objective of this paper was therefore to raise important questions concerning the interpretation of the different and heterogeneous data on the association between chlamydophila psittaci and ocular adnexal lymphoma, namely the impact of the methodology use ... | 2008 | 18690086 |
| familial outbreak of psittacosis as the first chlamydia psittaci infection reported from turkey. | psittacosis, also known as parrot disease, parrot fever and ornithosis, can be transmitted to humans by inhaling dried excreta of infected birds like canaries, parakeets, parrots, pigeons and turkeys. unless it is specifically thought of and investigated, the diagnosis of psittacosis can easily be missed and without identifying the exact etiology, the disease is usually treated as atypical pneumonia. we diagnosed psittacosis in a mother and her son with friedreich ataxia, who took care of two pa ... | 2008 | 18701984 |
| an enzyme-linked immunosorbent assay (elisa) for the detection of chlamydial antibody in duck sera. | an enzyme-linked immunosorbent assay (elisa) for the detection of chlamydial antibody in duck sera is described. the assay was used to test sera from a flock of domestic pekin ducks infected with chlamydia psittaci, and sera from wildfowl (mallard). sera from 63% of the domestic birds and 75% of the wildfowl exhibited chlamydial antibody titres of >/= 1/32, indicative of current or past chlamydial infection. there was a good correlation between the results obtained with the elisa and those obtai ... | 1983 | 18766767 |
| isolation in mccoy cells of chlamydia psittaci obtained from the domestic duck (anas platyrhyncos). | chlamydia psittaci has been isolated from several organs and conjunctivae of domestic pekin ducks. mccoy cells were susceptible to infection by this chlamydial strain as demonstrated by the formation of inclusion bodies within 48 hours post-inoculation. a good correlation was found between the isolation of c. psittaci from ducks and positive elisa serology results. | 1983 | 18766790 |
| bacterial flora of clinically normal conjunctivae in the domestic duckling. | a total of 100 eyes from newly hatched and young domestic ducklings were swabbed and the bacterial flora determined by standard bacteriological culture methods. of the total number of bacteria isolated, 57% were gram negative suggesting that the majority were derived from the intestinal flora. only 2% of the eyes sampled showed no bacterial growth. the bacterial species most commonly cultured were escherichia coli, staphylococcus epidermidis, acinetobacter anitratus, klebsiella pneumoniae, non-h ... | 1985 | 18766899 |
| isolation of chlamydia psittaci from avian sources using growth in cell culture. | a method is described for the isolation of chlamydia psittaci using cell culture treated with 5-iodo-2-deoxyuridine, and subsequent identification by direct fluorescent antibody staining. the method was applied to 110 sets of tissues from a variety of avian specimens submitted for diagnosis. chlamydiae were isolated and identified in 24 specimens: 13 from parrots, 7 from turkeys and 4 from pigeons. | 1978 | 18770373 |
| [rapid diagnosis of psittacosis using a recently developed real-time pcr technique]. | a 37-year-old man was admitted with cough and fever. three days after admission he was tested using a newly developed real-time pcr technique that detects the dna of chlamydophila psittaci. the result was positive; serological investigation was not positive until 14 days later. psittacosis is a potentially life-threatening infectious disease. laboratory diagnosis relies mainly on the assessment of paired sera, but this approach has obvious disadvantages in the acute setting. routine use of the r ... | 2008 | 18788681 |
| [an outbreak of psittacosis at a bird-fanciers fair in the netherlands]. | within 2 weeks after a bird-fanciers fair in the netherlands in november 2007 11 patients presented at our hospital with fever, shivers and severe headache. dyspnea and dry cough were less common, although the chest x-ray showed a consolidation in 9 out of 11 patients. the clinical diagnosis of psittacosis was quickly confirmed using real-time pcr, although the sensitivity of this test was low (20%). in 9 patients the diagnosis was later confirmed by a rise in complement fixing antibodies in pai ... | 2008 | 18788682 |
| chlamydia infection and lymphomas: association beyond ocular adnexal lymphomas highlighted by multiple detection methods. | chlamydia psittaci (cp) has been associated to ocular adnexal lymphomas (oal) with variable geographic distribution. herein, we used multiple chlamydia detection tools to identify cp elementary bodies-containing cell and to assess cp prevalence in both nodal and extranodal lymphomas. | 2008 | 18794089 |
| two fatal cases of psittacosis caused by chlamydophila psittaci. | two fatal cases of psittacosis are described in two poultry-processing-plant employees presenting with pneumonia and respiratory failure. diagnosis was confirmed by serological and pcr methods. psittacosis due to chlamydophila psittaci infection usually has a good recovery rate, although diagnostic delay and mistreatment can lead to severe complications and even death. this report emphasizes the need for rapid differential diagnosis and management of suspected cases of atypical pneumonia to prev ... | 2008 | 18809562 |
| dna microarray-based genotyping of chlamydophila psittaci strains from culture and clinical samples. | the avian and human pathogen chlamydophila (c.) psittaci represents a genetically heterogeneous species. to facilitate epidemiological surveys, more rapid yet highly specific molecular tests are needed. currently used typing methods, i.e. serotyping and pcr-rflp, have only limited sensitivity and are incapable of covering the wide spectrum of naturally occurring types of c. psittaci strains. in the present study, a new dna microarray assay based on the arraytube (at) technology was used to genot ... | 2009 | 18950965 |
| preliminary studies on the etiology of keratoconjunctivitis in reindeer (rangifer tarandus tarandus) calves in alaska. | keratoconjunctivitis outbreaks occur each summer in reindeer (rangifer tarandus tarandus) herds in western alaska, usa. this condition has not been well characterized nor has a definitive primary etiologic agent been identified. we evaluated the eyes of 660 calves near nome, alaska, between 29 june and 14 july 2005. clinical signs of keratoconjunctivitis were observed in 26/660 calves (3.9%). samples were collected from the conjunctival sac of both affected (n=22) and unaffected (n=24) animals f ... | 2008 | 18957667 |
| simultaneous zoonotic transmission of chlamydophila psittaci genotypes d, f and e/b to a veterinary scientist. | two groups of five 1-day-old conventional turkeys were housed in negative pressure stables to become experimentally infected with avian metapneumovirus (ampv) and ornithobacterium rhinotracheale (ort) at the age of 3 weeks. however, during the first 2 weeks, turkeys started to show respiratory disease characterized by rhinitis and dyspnoea. routine bacterial and viral diagnoses remained negative. therefore, pharyngeal swabs from the turkeys and from the veterinary scientist handling the animals ... | 2009 | 18963600 |
| evaluation of bioaerosol sampling techniques for the detection of chlamydophila psittaci in contaminated air. | chlamydophila (c.) psittaci, a category b bioterrorism agent, causes respiratory disease in birds and psittacosis or parrot fever in man. the disease spreads aerogenically and no vaccines are available for either birds or man. highly sensitive c. psittaci bioaerosol monitoring methods are unavailable. we evaluated: (1) dry filtration for collecting c. psittaci from contaminated air using different samplers and membrane filters, (2) impingement into different liquid collection media by use of the ... | 2009 | 18963601 |
| chlamydial infections in feral pigeons in europe: review of data and focus on public health implications. | feral pigeons (columba livia domestica), which thrive in most european towns and cities, are commonly infected with the zoonotic bacterium chlamydophila psittaci, the agent of psittacosis (also known as ornithosis) in humans. a number of surveys carried out over the last thirty years across europe have detected high seropositivity values and high percentages of infection in feral pigeon populations. overall, when considering data from 11 european countries, seropositivity values to c. psittaci i ... | 2009 | 18977610 |
| genotyping of chlamydophila psittaci by real-time pcr and high-resolution melt analysis. | human infection with chlamydophila (chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. c. psittaci is currently grouped into seven avian genotypes: a through f and e/b. serological testing, outer membrane protein a (ompa) gene sequencing, and restriction fragment length polymorphism analysis are currently used for distinguishing these genotypes. although accurate, these methods are time-consuming and require multi ... | 2009 | 19005152 |
| diabetes mellitus in a nanday conure (nandayus nenday). | a 3-year-old male nanday conure (nandayus nenday) was presented with acute lethargy, polyuria, and polydipsia. marked persistent hyperglycemia, glycosuria, and ketonuria were also noted. the serum insulin concentration (< 3 microu/ml) was lower than reference ranges described for other psittacine birds. initial treatment included insulin, antibiotics, and supportive care. insulin therapy was adjusted based on results of serial blood glucose curves. histopathologic examination of pancreatic biops ... | 2008 | 19014099 |
| chlamydophila psittaci infections in birds: a review with emphasis on zoonotic consequences. | the first part of the present review gives an overview on the history of infectious agents of the order chlamydiales and the general infection biology of chlamydophila (c.) psittaci, the causative agent of psittacosis. in the second part, the classification of c. psittaci strains, as well as issues of epidemiology of avian chlamydiosis., disease transmission routes, clinical disease, public health significance, present legislation and recommendations for prevention and control are reviewed. | 2009 | 19054633 |
| chlamydophila psittaci seropositivity and serum levels of soluble intercellular adhesion molecule-1 in farmers. | chlamydophila infection is known as an occupational hazard to veterinarians, farmers, poultry workers. serum levels of the soluble intercellular adhesion molecule-1 (sic am-1), is associated with c. seropositivity. since no data about ic am-1 levels and c. psittaci infection are known, the aim of this work was to assess if chronic persistent c. psittaci infection constantly stimulates the expression of sic am-1, independent of the characteristic symptoms of ornithosis. c. psittaci seropositivity ... | 2008 | 19061263 |
| ocular adnexal lymphoma and infectious agents. | 2009 | 19075420 | |
| comparison of the new inodiag automated fluorescence multiplexed antigen microarray to the reference technique in the serodiagnosis of atypical bacterial pneumonia. | the aetiological diagnosis of pneumonia depends largely on culture-, antigen- or pcr-based tests. atypical agents of pneumonia include coxiella burnetii, chlamydophila pneumoniae, chlamydia psittaci, legionella pneumophila, francisella tularensis and mycoplasma pneumoniae. in these cases, serological tests are commonly used for diagnosis. all of the above species were comparatively screened for by using the inodiag multiplexed automatic immunofluorescence assay and established reference techniqu ... | 2008 | 19076843 |
| transformation and isolation of allelic exchange mutants of chlamydia psittaci using recombinant dna introduced by electroporation. | to facilitate genetic investigations in the obligate intracellular pathogens chlamydia, the ability to construct variants by homologous recombination was investigated in c. psittaci 6bc. the single rrna operon was targeted with a synthetic 16s rrna allele, harboring three nucleotide substitutions over 398 bp, which imparts resistance to kasugamycin (ksm) and spectinomycin (spc) and causes loss of one hpai restriction site. a fourth, silent mutation was introduced 654 bp downstream in the beginni ... | 2009 | 19104068 |
| prevalence of chlamydophila psittaci-positive cloacal pcr tests in wild avian casualties in the uk. | 2009 | 19122217 |