Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| infection is the major trigger of hemophagocytic syndrome in adult patients treated with biological therapies. | hemophagocytic syndromes (hemophagocytic lymphohistiocytosis, hlh) are characterized by a wide range of etiologies, symptoms, and outcomes, but have a common etiopathogenic pathway leading to organ damage: an excessive inflammatory response. biological therapies have been proposed as a therapeutic option for refractory hlh, but have also been related to the development of hlh in severe immunosuppressed patients. | 2016 | 26277577 |
| phospholipase a1 modulates the cell envelope phospholipid content of brucella melitensis, contributing to polymyxin resistance and pathogenicity. | a subset of bacterial pathogens, including the zoonotic brucella species, are highly resistant against polymyxin antibiotics. bacterial polymyxin resistance has been attributed primarily to the modification of lipopolysaccharide; however, it is unknown what additional mechanisms mediate high-level resistance against this class of drugs. this work identified a role for the brucella melitensis gene bvea (bmeii0681), encoding a predicted esterase, in the resistance of b. melitensis to polymyxin b. ... | 2015 | 26282427 |
| patterns of hepatosplenic brucella abscesses on cross-sectional imaging: a review of clinical and imaging features. | while diffuse involvement of liver and spleen is frequently seen in brucellosis, suppurative abscesses caused by brucella are less common but well described. with the increased availability of cross-sectional imaging techniques, reports have become more frequent. four patients with hepatosplenic abscesses caused by brucella spp. are described and included in a review of 115 previously published cases. clinical characteristics and patterns on ultrasound (us) and computed tomography imaging were a ... | 2015 | 26283749 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| aorto-pulmonary fistula accompanied by root abscess and destruction of native aortic valve caused by brucellosis. | brucellosis endocarditis is a zoonosis infection of cardiovascular system with world-wide distribution, which is endemic in many provinces of the iran. the present report describes an exceptional case of fistulization between the aorta and pulmonary artery by brucella melitensis in a 34-year-old patient. he presented with the complaints of fever and weight loss and congestive heart failure. he was strongly positive for brucellosis by serological reaction and conventional microbiological cultures ... | 2015 | 26322294 |
| mass spectrometry data from proteomics-based screening of immunoreactive proteins of fully virulent brucella strains using sera from naturally infected animals. | here, we provide the dataset associated with our research article on comprehensive screening of brucella immunoreactive proteins using sera of naturally infected hosts published in biochemical and biophysical research communications wareth et al., 2015 [1]. whole-cell protein extracts were prepared from brucella abortus and brucella melitensis, separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and subsequently western blotting was carried out using sera from bo ... | 2015 | 26322324 |
| in situ characterization of splenic brucella melitensis reservoir cells during the chronic phase of infection in susceptible mice. | brucella are facultative intracellular gram-negative coccobacilli that chronically infect humans as well as domestic and wild-type mammals, and cause brucellosis. alternatively activated macrophages (m2a) induced by il-4/il-13 via stat6 signaling pathways have been frequently described as a favorable niche for long-term persistence of intracellular pathogens. based on the observation that m2a-like macrophages are induced in the spleen during the chronic phase of b. abortus infection in mice and ... | 2015 | 26376185 |
| simultaneous immunization of mice with omp31 and tf provides protection against brucella melitensis infection. | brucella vaccines consisting of live attenuated brucella strains are currently used in livestock, but safety concerns preclude their application in humans. subunit vaccines have recently emerged as safe and efficacious alternatives in both humans and animals. in this study, subunit vaccines were developed that consisted of a recombinant outer membrane protein (romp31) and the trigger factor chaperone protein (rtf) of brucella melitensis, either alone or in combination. balb/c mice that were immu ... | 2015 | 26384448 |
| a case of acute septic arthritis hip caused by brucella melitensis in an adolescent child. | brucella is among the most common zoonotic diseases affecting humans. although musculoskeletal involvement is seen in a large proportion of patients, the disease is often diagnosed late or misdiagnosed due to its subtle nature and rarity, and lack of awareness among clinicians. in this report, a 12-year-old girl was diagnosed with acute septic arthritis of the hip based on clinico-radiological features, and managed with standard treatment, including arthrotomy. however, the child did not respond ... | 2015 | 26392450 |
| survey of omp19 immunogenicity against brucella abortus and brucella melitensis: influence of nanoparticulation versus traditional immunization. | brucellosis is the most common zoonotic bacterial disease. prevention of human brucellosis is achieved through pasteurization of dairy products, appropriate sanitation and vaccination of domestic animals against the brucella species. b. abortus unlipidated 19 kda outer membrane protein (u-omp19) is a promising candidate for a subunit vaccine against brucellosis. this study investigates immunogenicity of omp19 alone and with freund's adjuvant (omp19-ifa) and n-trimethyl chitosan (tmc/omp19) nanop ... | 2015 | 26395469 |
| cd8+ t cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic brucella melitensis infection. | brucella melitensis is a well-adapted zoonotic pathogen considered a scourge of mankind since recorded history. in some cases, initial infection leads to chronic and reactivating brucellosis, incurring significant morbidity and economic loss. the mechanism by which b. melitensis subverts adaptive immunological memory is poorly understood. previous work has shown that brucella-specific cd8(+) t cells express gamma interferon (ifn-γ) and can transition to long-lived memory cells but are not polyfu ... | 2015 | 26416901 |
| endogenous endophthalmitis caused by brucella melitensis. | brucella is an intracellular gram-negative pathogen that acts as a facultative parasite. b. melitensis endogenous endophthalmitis is quite rare. we herein report an unusual case of b. melitensis endophthalmitis with a good outcome after appropriate management. | 2016 | 26444521 |
| brucellosis outbreak in a rural endemic region of mexico - a comprehensive investigation. | brucellosis is a worldwide zoonotic disease. generally, humans can be infected by either the consumption of raw milk and fresh cheeses made from unpasteurised milk or by contact with infected animals, mainly in endemic regions. in this study, we investigated a brucellosis outbreak in state of guanajuato, an endemic region of mexico. microbiological culture of human blood, raw milk from cows and goats, and fresh cheeses was performed to isolate brucella. identification of the bacteria was done by ... | 2015 | 26455370 |
| genetic characterization of brucella melitensis and brucella abortus geographical clusters in italy. | the genetic diversity of brucella melitensis and brucella abortus strains isolated in 199 cattle and sheep from 156 brucellosis outbreaks which occurred in 8 regions of southern italy in 2011, was determined using a multiple-locus variable number of tandem repeats analysis approach. the existence of possible genetic clusters was verified through a hierarchical cluster analysis based on 'single link', which is closely related to the minimum spanning tree. the hamming weighted distance matrix was ... | 2015 | 26455376 |
| etiological diagnosis of community-acquired pneumonia in adult patients: a prospective hospital-based study in mashhad, iran. | pneumonia is the third most common cause of death in the world, and mortality is highest for patients who require hospitalization. | 2015 | 26464771 |
| serological diagnosis of bovine brucellosis using b. melitensis strain b115. | bovine brucellosis is diagnosed by official tests, such as rose bengal plate test (rbpt) and complement fixation test (cft). both tests detect antibodies directed against the lipolysaccharide (lps) of brucella cell wall. despite their good sensitivity, those tests do not discriminate between true positive and false positive serological reactions (fpsr), the latter being generated by animals infected with other gram negative microorganisms that share components of brucella lps. in this study, an ... | 2015 | 26476137 |
| novel vaccine candidates against brucella melitensis identified through reverse vaccinology approach. | global health therapeutics is a rapidly emerging facet of postgenomics medicine. in this connection, brucella melitensis is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. presently, no licensed vaccines are available for human brucellosis. here, we report the identification of potential vaccine candidates against b. melitensis using a reverse vaccinology approach. based on a systematic screening of exoproteome and secretome of b. melitensis 16 m, we identifi ... | 2015 | 26479901 |
| brucella infection of the thoracic vertebral arch presenting with an epidural abscess: a case report. | although brucella spondylitis and brucella discitis have been frequently reported, brucella infection of the vertebral arch is rare and has not been previously described. we present the first case of brucella infection of the thoracic vertebral arch with epidural abscess formation and discuss the clinical key points. | 2015 | 26496896 |
| systematic review of brucellosis in the middle east: disease frequency in ruminants and humans and risk factors for human infection. | a systematic review of studies providing frequency estimates of brucellosis in humans and ruminants and risk factors for brucella spp. seropositivity in humans in the middle east was conducted to collate current knowledge of brucellosis in this region. eight databases were searched for peer-reviewed original arabic, english, french and persian journal articles; the search was conducted on june 2014. two reviewers evaluated articles for inclusion based on pre-defined criteria. of 451 research art ... | 2016 | 26508323 |
| detection and discrimination of two brucella species by multiplex real-time pcr and high-resolution melt analysis curve from human blood and comparison of results using rflp. | rapid and accurate detection of brucella abortus and brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. this study reveals a new method in detection of clinical samples of brucellosis using real-time pcr and high-resolution melt (hrm) curve analysis. | 2015 | 26523223 |
| acute abdomen caused by brucellar hepatic abscess. | brucellosis, a zoonosis that is common worldwide, is endemic in many countries, primarily those of the mediterranean region (including turkey). human brucellosis is a systemic infection with a wide clinical spectrum. although hepatic involvement is very common during the course of chronic brucellosis, hepatic abscess is a very rare complication of brucella spp. infection. we present a case of hepatic abscess caused by brucella melitensis, which resembled the clinical presentation of surgical acu ... | 2016 | 26526924 |
| serological diagnostic potential of recombinant outer membrane proteins (romps) from brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay. | brucella melitensis is the most important pathogenic species of brucella spp. which affects goats and sheep and causes caprine and ovine brucellosis, respectively. serological tests for diagnosis of brucellosis such as rose bengal plate test (rbpt) and enzyme-linked immunosorbent assay (elisa) usually utilize smooth lipopolysaccharides (s-lps) as a diagnostic antigen which could give false positive serological reactions. outer membrane proteins (omp) of b. melitensis have been used as alternativ ... | 2015 | 26530141 |
| immunization with individual proteins of the lrp/asnc family induces protection against brucella melitensis 16m challenges in mice. | brucellosis is one of the most common zoonoses worldwide. subunit vaccines are promising for the prevention of human brucellosis. in our previous protective antigen screening studies, we identified a new protective antigen, bmei0357, which belongs to the lrp/asnc protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. in the present study, the brucella genome annotation was screened and a total of six proteins were identified as members of the lrp/asnc fam ... | 2015 | 26579099 |
| rocket and two dimensional immunoelectrophoresis in diagnosis of caprine brucellosis. | brucellosis is a major bacterial zoonosis of global importance with the causative organisms of gram-negative facultative intracellular pathogens. the aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. | 2015 | 26587475 |
| [brucella melitensis is an agent in pediatric arthritis: case series]. | 2015 | 26593815 | |
| brucella melitensis 16mδtcfsr as a potential live vaccine allows for the differentiation between natural and vaccinated infection. | brucellosis is a zoonotic disease that poses a serious threat to public health and safety. although the live attenuated vaccines targeting brucellosis, such as m5-90, are effective, there are a number of drawbacks to their use. for example, the vaccines are unable to differentiate between the natural and vaccinated forms of the infection, and these vaccines have also been shown to cause abortion in pregnant animals. therefore, a safer and more potent vaccine is required. in the present study, a ... | 2015 | 26622461 |
| innocuity and immune response to brucella melitensis rev.1 vaccine in camels (camelus dromedarius). | a field trial was conducted in a camel brucellosis-free herd to evaluate antibody response to the brucella melitensis rev.1 vaccine in camels and assess shedding of the vaccine strain in milk. twenty eight camels were divided into four groups according to their age and vaccination route. groups a (n=3) and b (n=3) consisted of non-pregnant lactating female camels, vaccinated through subcutaneous and conjunctival routes, respectively. groups c (n=10) consisted of 8-11 months old calves vaccinated ... | 2014 | 26623347 |
| [undulant fever and autoimmune hemolytic anemia in a 20-year-old exchange student from jordan - the human brucellosis as an important differential diagnosis in migrants]. | a 20-year-old jordanian exchange student presents with recurrent fever, night sweats, cough, and swelling and redness around the ankle. physical examination further reveals bilateral ankle arthritis and painful cervical lymphadenopathy. | 2015 | 26625239 |
| brucella suis strain 2 vaccine is safe and protective against heterologous brucella spp. infections. | brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. brucella abortus, brucella melitensis and brucella suis are the major pathogenic species to humans. vaccination with live attenuated b. suis strain 2 (s2) vaccine is an essential and critical component in the control of brucellosis in china. the s2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. however, there a ... | 2016 | 26626213 |
| multiple-locus variable-number tandem-repeat analysis of brucella isolates from patients in xinjiang china. | this study is to characterize and identify the human brucella strains in xinjiang, china with multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) scheme. | 2015 | 26629067 |
| a retrospective study of 39 patients treated with anterior approach of thoracic and lumbar spondylodiscitis: clinical manifestations, anterior surgical treatment, and outcome. | the aim of this study is to report our 39 patients treated with anterior debridement and autologous iliac bone grafting with or without anterior instrumentation, which is the presumed treatment of choice for thoracic or lumbar spondylodiscitis.our patients underwent surgical treatment of spondylodiscitis using anterior debridement and autologous iliac bone grafting with or without anterior instrumentation and were analyzed with a mean follow-up of 8 years (range, 2-11 years). kaneda 2-rod system ... | 2015 | 26632729 |
| molecular study of brucellosis in camels by the use of taqman® real-time pcr. | brucellosis is a zoonosis of economic importance that reduces productivity in livestock enterprises as it induces abortion in infected animals. a study was designed aimed at detecting brucella in blood and lymph node specimens from camels by the use of real-time pcr in iran. sample collection and dna extraction were done on blood (n = 135) and lymph node (n = 135) samples collected from 135 camels (abattoir survey) from both sexes at various ages in different seasons. the real-time pcr for speci ... | 2015 | 26689877 |
| comparative transcriptome analysis of brucella melitensis in an acidic environment: identification of the two-component response regulator involved in the acid resistance and virulence of brucella. | brucella melitensis, encounters a very stressful environment in phagosomes, especially low ph levels. so identifying the genes that contribute to the replication and survival within an acidic environment is critical in understanding the pathogenesis of the brucella bacteria. in our research, comparative transcriptome with rna-seq were used to analyze the changes of genes in normal-medium culture and in ph4.4-medium culture. the results reveal that 113 genes expressed with significant differences ... | 2016 | 26691825 |
| typing discrepancy between phenotypic and molecular characterization revealing an emerging biovar 9 variant of smooth phage-resistant b. abortus strain 8416 in china. | a newly isolated smooth colony morphology phage-resistant strain 8416 isolated from a 45-year-old cattle farm cleaner with clinical features of brucellosis in china was reported. the most unusual phenotype was its resistance to two brucella phages tbilisi and weybridge, but sensitive to berkeley 2, a pattern similar to that of brucella melitensis biovar 1. vitek 2 biochemical identification system found that both strain 8416 and b. melitensis strains shared positive ilatk, but negative in other ... | 2015 | 26696984 |
| identification of phosphoribosyl-amp cyclohydrolase, as drug target and its inhibitors in brucella melitensis bv. 1 16m using metabolic pathway analysis. | brucella melitensis is a pathogenic gram-negative bacterium which is known for causing zoonotic diseases (brucellosis). the organism is highly contagious and has been reported to be used as bioterrorism agent against humans. several antibiotics and vaccines have been developed but these antibiotics have exhibited the sign of antibiotic resistance or ineffective at lower concentrations, which imposes an urgent need to identify the novel drugs/drug targets against this organism. in this work, meta ... | 2017 | 26725317 |
| rna-seq reveals the critical role of cspa in regulating brucella melitensis metabolism and virulence. | brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. the ability of brucella to survive and multiply in the hostile environment of host macrophages is essential for its virulence. the cold shock protein cspa plays an important role in the virulence of b. melitensis. to analyze the genes regulated by cspa, the whole transcriptomes of b. melitensis niδcspa and its parental wild-type strain, b. melitensis ni, were sequenced and analyzed using the solexa/i ... | 2016 | 26740105 |
| nasal vaccination stimulates cd8(+) t cells for potent protection against mucosal brucella melitensis challenge. | brucellosis remains a significant zoonotic threat worldwide. humans and animals acquire infection via their oropharynx and upper respiratory tract following oral or aerosol exposure. after mucosal infection, brucellosis develops into a systemic disease. mucosal vaccination could offer a viable alternative to conventional injection practices to deter disease. using a nasal vaccination approach, the δznua b. melitensis was found to confer potent protection against pulmonary brucella challenge, and ... | 2016 | 26752510 |
| in silico design, cloning and high level expression of l7/l12-tomp31 fusion protein of brucella antigens. | globally, brucella melitensis and b. abortus are the most common cause of human brucellosis. the outer membrane protein 31 (omp31) and l7/l12 are immunodominant and protective antigens conserved in human brucella pathogens which are considered as potential vaccine candidates. we aimed to design the fusion protein from brucella l7/l12 and truncated omp31proteins, in silico, clone the fusion in pet28a vector, and express it in escherichia coli host. two possible fusion forms, l7/l12-tomp31 and tom ... | 2017 | 26752992 |
| prevalence and risk factors for foot and mouth disease infection in small ruminants in israel. | during the last decade, 27% of the foot and mouth disease (fmd) outbreaks in israel affected small ruminant (sr) farms. fmd outbreaks reoccur in israel despite vaccination of all livestock and application of control measures. we performed a cross-sectional serological study, aimed at estimating the prevalence of fmd infection in sr in israel and the possible risk factors for infection. overall, 2305 samples of adult sheep (n=1948) and goats (n=357) were collected during 2011-14 in two separate s ... | 2016 | 26774447 |
| characterization of ribonuclease iii from brucella. | bacterial ribonuclease iii (rnase iii) is a highly conserved endonuclease, which plays pivotal roles in rna maturation and decay pathways by cleaving double-stranded structure of rnas. here we cloned rncs gene from the genomic dna of brucella melitensis, and analyzed the cleavage properties of rnase iii from brucella. we identified brucella-encoding small rna (srna) by high-throughput sequencing and northern blot, and found that srna of brucella and homo mirna precursor (pre-mirna) can be bound ... | 2016 | 26778206 |
| outbreaks of brucellosis related to the consumption of unpasteurized camel milk. | brucellosis is the most frequent zoonosis reported in qatar, mainly related to exposure to infected camels. an outbreak of human brucellosis in 14 members of a family living in a rural area in qatar is reported herein. clinical, epidemiological and laboratory results from all 14 patients with brucella and 12 non-confirmed family members were collected from files. all patients reported fever for a maximum of 14 days, associated with arthralgia (6 patients), weakness (4 patients), headache (4 pati ... | 2017 | 26796768 |
| [the effects of repetitive extragenic palindromic sequences from brucella melitensis dna on the toll-like receptor 9-mediated interferon-α production]. | to screen the repetitive extragenic palindromic sequences with activation of toll-like receptor 9 (tlr9) activity from brucella melitensis dna, providing new ideas and new targets for prevention and treatment of brucellosis. | 2015 | 26813141 |
| vaccination control programs for multiple livestock host species: an age-stratified, seasonal transmission model for brucellosis control in endemic settings. | brucella melitensis causes production losses in ruminants and febrile disease in humans in africa, central asia, the middle east and elsewhere. although traditionally understood to affect primarily sheep and goats, it is also the predominant brucella species that affects cows in some endemic areas. despite this, no licensed vaccine is available specifically for use against b. melitensis in cows. the mainstay of most control programs is vaccination of sheep and goats with a live vaccine, rev-1. t ... | 2016 | 26825313 |
| immunogenicity and protective efficacy of brucella abortus recombinant protein cocktail (romp19+rp39) against b. abortus 544 and b. melitensis 16m infection in murine model. | in this study, the immunogenicity and protective efficacy of recombinant proteins omp19 (ro) and p39 (rp) from brucella abortus were evaluated individually and compared with the cocktail protein (ro+rp) against b. abortus 544 and brucella melitensis 16m infection in balb/c mouse model. intra-peritoneal (i.p.) immunization with ro+rp cocktail developed substantially higher antibody titers predominant with th1 mediated isotypes (igg2a/2b). western blot analysis using anti-ro+rp antibodies showed s ... | 2016 | 26826463 |
| evaluation of quantum dot immunofluorescence and a digital cmos imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarrays. | organic fluorescent dyes are widely used for the visualization of bound antibody in a variety of immunofluorescence assays. however, the detection equipment is often expensive, fragile, and hard to deploy widely. quantum dots (qdot) are nanocrystals made of semiconductor materials that emit light at different wavelengths according to the size of the crystal, with increased brightness and stability. here, we have evaluated a small benchtop "personal" optical imager (arraycam) developed for quanti ... | 2016 | 26842269 |
| analysis of the first temperate broad host range brucellaphage (bipbo1) isolated from b. inopinata. | brucella species are important human and animal pathogens. though, only little is known about mobile genetic elements of these highly pathogenic bacteria. to date, neither plasmids nor temperate phages have been described in brucellae. we analyzed genomic sequences of various reference and type strains and identified a number of putative prophages residing within the brucella chromosomes. by induction, phage bipbo1 was isolated from brucella inopinata. bipbo1 is a siphovirus that infects several ... | 2016 | 26858702 |
| antibacterial and antibiofilm effects of boron on different bacteria. | boron (b) compounds are used in many fields ranging from medicine to industry. in this study, boric acid (ba) and disodium octaborate tetrahydrate (dot) were evaluated for their antibacterial effects and antibiofilm capacities on selected strains of clinical and type cultures that are of veterinary concern (staphylococcus aureus atcc 25923, aeromonas hydrophila atcc 19570, pseudomonas aeruginosa atcc 27853, brucella melitensis rev1 and field isolates of vibrio anguillarum, aeromonas hydrophila, ... | 2016 | 26864941 |
| structural studies of lipopolysaccharide-defective mutants from brucella melitensis identify a core oligosaccharide critical in virulence. | the structures of the lipooligosaccharides frombrucella melitensismutants affected in the wbkd and manbcoreproteins have been fully characterized using nmr spectroscopy. the results revealed that disruption ofwbkdgives rise to a rough lipopolysaccharide (r-lps) with a complete core structure (β-d-glcp-(1→4)-α-kdop-(2→4)[β-d-glcpn-(1→6)-β-d-glcpn-(1→4)[β-d-glcpn-(1→6)]-β-d-glcpn-(1→3)-α-d-manp-(1→5)]-α-kdop-(2→6)-β-d-glcpn3n4p-(1→6)-α-d-glcpn3n1p), in addition to components lacking one of the ter ... | 2016 | 26867577 |
| delta-pgm, a new live-attenuated vaccine against brucella suis. | brucellosis is one of the most widespread zoonosis in the world affecting many domestic and wild animals including bovines, goats, pigs and dogs. each species of the brucella genus has a particular tropism toward different mammals being the most relevant for human health brucella abortus, brucella melitensis and brucella suis that infect bovines, goats/camelids and swine respectively. although for b. abortus and b. melitensis there are vaccines available, there is no efficient vaccine to protect ... | 2016 | 26899373 |
| acute brucellosis following accidental exposure to brucella melitensis rev 1 vaccine. | brucellosis is mainly transmitted to humans by direct contact with infected animals, consumption of non-pasteurized dairy products or through inhalation of aerosols. however, the disease may also be transmitted by exposure to brucella vaccination that is used in veterinary medicine. in the literature, there were a few case reports of persons who developed brucellosis after unintentional inoculation or conjunctival exposure to the live brucella vaccine. here, we describe a sheep farmer with acute ... | 2015 | 26907981 |
| the brucella melitensis m5-90 phosphoglucomutase (pgm) mutant is attenuated and confers protection against wild-type challenge in balb/c mice. | brucellae are gram-negative intracellular bacterial pathogens that infect humans and animals, bringing great economic burdens to developing countries. live attenuated brucella vaccines (strain m5-90 or others) are the most efficient means for prevention and control of animal brucellosis. however, these vaccines have several drawbacks, including residual virulence in animals, and difficulties in differentiating natural infection from vaccine immunization, which limit their application. a vaccine ... | 2016 | 26925620 |
| brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (vulpes vulpes). | two slow-growing, gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains f60t and f965), isolated in austria from mandibular lymph nodes of two red foxes (vulpes vulpes), were subjected to a polyphasic taxonomic analysis. in a recent study, both isolates were assigned to the genus brucella but could not be attributed to any of the existing species. hence, we have analysed both strains in further detail to determine their exact taxonomic position and genetic relatedness to other ... | 2016 | 26928956 |
| the first report of brucella suis biovar 1 isolation in human in turkey. | brucella melitensis and b. abortus are the species generally isolated from human samples in turkey. several studies have also demonstrated the presence of antibodies against b. canis. | 2017 | 26947547 |
| cxcr2 mediates brucella-induced arthritis in interferon γ-deficient mice. | brucella species are facultative intracellular gram-negative bacteria that cause brucellosis, a common global zoonosis. infection of the joints is the most common focal complication of brucellosis in humans. the purpose of this study was to identify mediators of focal inflammation during brucellosis. | 2016 | 26951819 |
| a review of the basis of the immunological diagnosis of ruminant brucellosis. | bacteria of the genus brucella cause brucellosis, the most common bacterial zoonosis worldwide. the diagnosis of brucella abortus and brucella melitensis ruminant brucellosis is based on bacteriological and immunological tests, the latter being routinely used in control and eradication and surveillance programs. infections by smooth and rough brucella spp., the use of smooth and rough vaccines, and the false-positive serological reactions caused by yersinia enterocolitica o:9 and other cross-rea ... | 2016 | 26964721 |
| [epidemiology of caprine and ovine brucellosis in formosa province, argentina]. | an epidemiological study of brucellosis was carried out in 516 goats and mixed flocks (goat/sheep) from the three agro-ecological regions of formosa province, argentina. serum samples from a total of 25401 goats and 2453 sheeps were analyzed using buffered plate agglutination test (bpat) and complement fixation test (cft). bacteriological and pcr analyses on milk samples from goats in three flocks with a history of brucellosis and recent abortions were also performed. brucellosis was detected in ... | 2017 | 26969324 |
| construction of an expression plasmid (vector) encoding brucella melitensis outer membrane protein, a candidate for dna vaccine. | dna vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. bacteria of the genus brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. | 2013 | 26989713 |
| genotyping of brucella melitensis strains from dromedary camels (camelus dromedarius) from the united arab emirates with multiple-locus variable-number tandem repeat analysis. | camel brucellosis is a widespread zoonotic disease in camel-rearing countries caused by brucella melitensis and brucella abortus. the aim of this study was the first genetic analysis of b. melitensis strains isolated from dromedary camels (camelus dromedarius) using multiple-locus variable-number tandem repeat analysis (mlva). mlva 16 and its mlva 8 and mlva11 subsets were used to determine the genotypes of 15 b. melitensis isolates from dromedary camels (11 strains) and other host species (4 st ... | 2016 | 27016751 |
| serologic and molecular evidence of brucella ovis infection in ovine and caprine flocks in the state of minas gerais, brazil. | brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. this study aimed to determine the frequency of b. ovis positivity in rams and goats flocks in the state of minas gerais, brazil, by agarose gel immunodiffusion (agid), elisa, rose bengal, pcr and bacteriological isolation as diagnostic tools. | 2016 | 27017445 |
| [features of mass-spectrometric protein profiles of strains of brucellosis causative agent during preparation of culture on various nutrient media]. | carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (maldi-tof ms) of protein profiles of brucellosis causative agents (brucella melitensis rev-1 and brucella abortus 19ba), cultivated in various nutrient media: albimi agar, brucellagar and erythrit-agar. | 2016 | 27029113 |
| identification of immune effectors essential to the control of primary and secondary intranasal infection with brucella melitensis in mice. | the mucosal immune system represents the first line of defense against brucella infection in nature. we used genetically deficient mice to identify the lymphocytes and signaling pathways implicated in the control of primary and secondary intranasal infection with b. melitensis our analysis of primary infection demonstrated that the effectors implicated differ at the early and late stages and are dependent on the organ. tcr-δ, tap1, and il-17ra deficiency specifically affects early control of bru ... | 2016 | 27036913 |
| epidemiological survey of brucellosis in sheep and goats in selected pastoral and agro-pastoral lowlands of ethiopia. | an epidemiological survey was conducted in pastoral regions of ethiopia to investigate the distribution of brucellosis in sheep and goats. between november 2004 and december 2007, a total of 6,201 serum samples were collected from 67 randomly selected peasant associations, 25 districts and eight pastoral zones of ethiopia. the rose bengal plate test (rbpt) and complement fixation test were used in series. samples for bacteriology were collected from three export abattoirs, where 285 goats were r ... | 2015 | 27044159 |
| [expression and identification of eukaryotic expression vectors of brucella melitensis lipoprotein omp19]. | to construct eukaryotic expression vectors carrying brucella melitensis outer membrane protein 19 (omp19), express them in transfected huh7.5.1 and jeg-3 cells, and analyze their role in cell apoptosis. | 2016 | 27053612 |
| large-scale identification of small noncoding rna with strand-specific deep sequencing and characterization of a novel virulence-related srna in brucella melitensis. | brucella is the causative agent of brucellosis, a worldwide epidemic zoonosis. small noncoding rnas (srnas) are important modulators of gene expression and involved in pathogenesis and stress adaptation of brucella. in this study, using a strand-specific rna deep-sequencing approach, we identified a global set of srnas expressed by b. melitensis 16m. in total, 1321 srnas were identified, ranging from 100 to 600 nucleotides. these srnas differ in their expression levels and strand and chromosomal ... | 2016 | 27112796 |
| brucellosis outbreak in children and adults in two areas in israel. | two parallel outbreaks of brucella melitensis infection occurred in 2014 in two geographical areas in israel. in two medical centers in northern israel and one medical center in jerusalem, 102 patients (58 children, 47 adults) were diagnosed with brucellosis. most patients (n = 76, 72%) were muslim arabs, 28 (27%) were druze, and one was jewish. the source of infection was often traced to cheese from the palestinian authority. biovar-1 was evident in 98% in northern israel but only in 42% in jer ... | 2016 | 27114301 |
| a novel recombinant multi-epitope protein against brucella melitensis infection. | live, attenuated brucella vaccines are considered effective but can induce abortions in pregnant animals and are potentially infectious to humans. there is a strong need to improve the immunoprotective effects and safety of vaccines against brucella. currently, subunit vaccines have been demonstrated to be safe and efficacious alternatives in both humans and animals. in this study, we employed bioinformatics tools to predict b and t cell epitopes to aid development of a novel recombinant multi-e ... | 2016 | 27133932 |
| first case of human brucellosis caused by brucella melitensis in korea. | 2016 | 27139618 | |
| comprehensive identification of immunodominant proteins of brucella abortus and brucella melitensis using antibodies in the sera from naturally infected hosts. | brucellosis is a debilitating zoonotic disease that affects humans and animals. the diagnosis of brucellosis is challenging, as accurate species level identification is not possible with any of the currently available serology-based diagnostic methods. the present study aimed at identifying brucella (b.) species-specific proteins from the closely related species b. abortus and b. melitensis using sera collected from naturally infected host species. unlike earlier reported investigations with eit ... | 2016 | 27144565 |
| brucella melitensis and mycobacterium tuberculosis depict overlapping gene expression patterns induced in infected thp-1 macrophages. | pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts' defensive mechanisms. in this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their host's killing attitude. studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. brucellosis, caused by the brucella strains, is a zoonotic bacterial disea ... | 2015 | 27175205 |
| cases of human brucellosis in sweden linked to middle east and africa. | human brucellosis cases are still reported each year in sweden despite eradication of the disease in animals. epidemiological investigation has never been conducted to trace back the source of human infection in the country. the purpose of the study was to identify the source of infection for 16 human brucellosis cases that occurred in sweden, during the period 2008-2012. | 2016 | 27188619 |
| [a case of brucellosis with intermittent fever in a patient returning from france]. | we herein report on a 62-year-old man who presented with symptoms of intermittent fever that persisted after returning from a trip to france. during his trip, he had eaten natural cheese. although no bacteria could be isolated from blood culture, the serum agglutination test showed a positive antibody titer of 1 : 160 for brucella canis. the patient responded well to combination antibiotic therapy consisting of gentamicin, rifampicin, and doxycycline, and his symptoms improved. he became antibod ... | 2016 | 27197442 |
| latex agglutination using the periplasmic proteins antigen of brucella melitensis is a successful, rapid, and specific serodiagnostic test for ovine brucellosis. | brucellosis, especially caused by brucella melitensis, is considered the most-widespread zoonosis in the world, particularly in developing countries. this study was planned to develop an accurate test for diagnosis of ovine brucellosis using a specific hot saline extracted soluble brucella melitensis periplasmic proteins (sbpps). the efficacy of the latex agglutination test (lat) using sbpps compared to the rose bengal test (rbt), buffered plate agglutination test (bpat), serum agglutination tes ... | 2016 | 27207442 |
| [brucella melitensis (bm) bacteremia in hospitalized adult patients in southern israel]. | southern israel is endemic for brucella melitensis (bm) infection. | 2016 | 27215118 |
| omics of brucella: species-specific srna-mediated gene ontology regulatory networks identified by computational biology. | brucella is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. brucella species are currently intensively studied with a view to developing novel global health diagnostics and therapeutics. in this context, small rnas (srnas) are one of the emerging topical areas; they play significant roles in regulating gene expression and cellular processes in bacteria. in the present study, we forecast srnas in three brucella species that infect humans, namely brucella melit ... | 2016 | 27223678 |
| brucella tir-like protein tcpb/btp1 specifically targets the host adaptor protein mal/tirap to promote infection. | brucella spp. are known to avoid host immune recognition and weaken the immune response to infection. brucella like accomplish this by employing two clever strategies, called the stealth strategy and hijacking strategy. the tir domain-containing protein (tcpb/btp1) of brucella melitensis is thought to be involved in inhibiting host nf-κb activation by binding to adaptors downstream of toll-like receptors. however, of the five tir domain-containing adaptors conserved in mammals, whether myd88 or ... | 2016 | 27311859 |
| an analysis of children with brucellosis associated with haemophagocytic lymphohistiocytosis. | this retrospective study included seven paediatric cases aged from 4 to 14 (10.2±3.4) years with pathologically proved haemophagocytic lymphohistiocytosis from a single institution during 2009 and 2013. over this time period, 496 patients with brucellosis were diagnosed. none of the patients (3 boys and 4 girls) had a history of any haematologic disorder. all patients had an anamnesis for recently consumed unpasteurised homemade dairy products or had a contact history with sheep and/or cows. the ... | 2016 | 27367321 |
| activation of bovine neutrophils by brucella spp. | brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus brucella. while many species of brucella exist, brucella melitensis, brucella abortus, and brucella suis are the most common pathogens of humans and livestock. the virulence of brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. the aim of this study was to characterize the bo ... | 2016 | 27436438 |
| an nmr-guided screening method for selective fragment docking and synthesis of a warhead inhibitor. | selective hits for the glutaredoxin ortholog of brucella melitensis are determined using std nmr and verified by trnoe and (15)n-hsqc titration. the most promising hit, rk207, was docked into the target molecule using a scoring function to compare simulated poses to experimental data. after elucidating possible poses, the hit was further optimized into the lead compound by extension with an electrophilic acrylamide warhead. we believe that focusing on selectivity in this early stage of drug disc ... | 2016 | 27438815 |
| biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use. | the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ... | 2016 | 27482880 |
| isolation & characterization of brucella melitensis isolated from patients suspected for human brucellosis in india. | brucellosis is endemic in the southern part of india. a combination of biochemical, serological and molecular methods is required for identification and biotyping of brucella. the present study describes the isolation and biochemical, molecular characterization of brucella melitensis from patients suspected for human brucellosis. | 2016 | 27488010 |
| development and application of dot-enzyme-linked immunosorbent (dot-elisa) assay for detection of brucella melitensis and evaluation of the shedding pattern in infected goats. | early and accurate diagnosis of brucella melitensis is essential for the treatment and control of brucellosis both in animals and humans. the thrust for the development of a rapid diagnostic technique to overcome the limitations of conventional microbiological and serological tests brought about this investigation on the development and application of dot-elisa for antigen and antibody detection in infected goats. fifteen apparently healthy boer aged 2-3 years which tested negative for brucellos ... | 2017 | 27494045 |
| immune response induced by conjunctival immunization with polymeric antigen blsomp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis. | control of ovine brucellosis with subcellular vaccines can solve some drawbacks associated with the use of brucella melitensis rev.1. previous studies have demonstrated that the polymeric antigen blsomp31 administered by parenteral route was immunogenic and conferred significant protection against b. ovis in rams. immunization with blsomp31 by conjunctival route could be efficient for the induction of mucosal and systemic immune responses. in this work, we evaluated the conjunctival immunization ... | 2016 | 27496742 |
| comparing rapid and specific detection of brucella in clinical samples by pcr-elisa and multiplex-pcr method. | rapid diagnosis and differentiation of brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. this study aimed to identify and compare pcr-elisa as a more accurate diagnositc test with other common molecular and serological tests. | 2016 | 27499776 |
| brucella melitensis methionyl-trna-synthetase (metrs), a potential drug target for brucellosis. | we investigated brucella melitensis methionyl-trna-synthetase (bmmetrs) with molecular, structural and phenotypic methods to learn if bmmetrs is a promising target for brucellosis drug development. recombinant bmmetrs was expressed, purified from wild type brucella melitensis biovar abortus 2308 strain atcc/crp #dd-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-trna-synthetase inhibitors of the blood stage form of trypanosoma bru ... | 2016 | 27500735 |
| antimicrobial susceptibility testing of brucella melitensis isolated from patients with acute brucellosis in a centre of iran. | structured to purpose: human brucellosis is one of the most common zoonotic infections worldwide, which remains one of the major problems for public health. despite the world health organization's recommendation for human brucellosis treatment, sporadic cases of relapse have been reported. the aim of this study was to assess the susceptibility of brucella isolates to common antibiotics that are prescribed by the physician for the treatment of brucellosis and also to determine the minimum inhibit ... | 2017 | 27514957 |
| genotyping of brucella melitensis and brucella abortus strains currently circulating in xinjiang, china. | brucellosis is a well-known zoonotic disease that can cause severe economic and healthcare losses. xinjiang, one of the biggest livestock husbandry sectors in china, has gone through increasing incidence of brucellosis in cattle and small ruminants recently. in this paper, 50 b. melitensis strains and 9 b. abortus strains collected from across xinjiang area (from 2010 to 2015) were genotyped using multiple locus variable-number tandem-repeat (vntr) analysis (mlva) and multi-locus sequence typing ... | 2016 | 27521159 |
| genetic characterization and comparative genome analysis of brucella melitensis isolates from india. | brucellosis is the most frequent zoonotic disease worldwide, with over 500,000 new human infections every year. brucella melitensis, the most virulent species in humans, primarily affects goats and the zoonotic transmission occurs by ingestion of unpasteurized milk products or through direct contact with fetal tissues. brucellosis is endemic in india but no information is available on population structure and genetic diversity of brucella spp. in india. we performed multilocus sequence typing of ... | 2016 | 27525259 |
| brucellosis in a refugee who migrated from syria to germany and lessons learnt, 2016. | a teenage woman migrating from syria arrived in may 2015 in germany. she gave birth to a healthy child in early 2016, but became febrile shortly after delivery. blood cultures revealed brucella melitensis. in retrospect, she reported contact with sheep in syria and recurrent pain in the hip joints over about five months before diagnosis of brucellosis. we discuss consequences for adequate treatment of mother and child as well as for clinical and laboratory management. | 2016 | 27525454 |
| the abc transporter yejabef is required for resistance to antimicrobial peptides and the virulence of brucella melitensis. | the ability to resist the killing effects of host antimicrobial peptides (amps) plays a vital role in the virulence of pathogens. the brucella melitensis ni genome has a gene cluster that encodes abc transport. in this study, we constructed yeja1, yeja2, yejb, yeje, yejf, and whole yej operon deletion mutants, none of which exhibited discernible growth defect in tsb or minimal medium. unlike their parental strain, the mutants showed a significantly increased sensitivity to acidic stress. the niδ ... | 2016 | 27550726 |
| endovascular management of concomitant thoracic and abdominal aortic ruptures resulting from brucellosis aortitis. | acute aortic symptomatology is an unusual manifestation of brucella melitensis infection. we present a rare case of acute multifocal thoracic and abdominal aortic ruptures arising from brucellosis aortitis managed exclusively with endovascular surgery. | 2017 | 27554701 |
| clinical and diagnostic aspects of brucellosis and antimicrobial susceptibility of brucella isolates in hamedan, iran. | current drug regimens for brucellosis are associated with relatively high rates of therapeutic failure or relapse. reduced antimicrobial susceptibility of brucella spp. has been proposed recently as a potential cause of therapeutic failure. the aim of this study was to evaluate the antibiotic resistance pattern of brucella melitensis clinical isolates by e-test method in hamadan, west of iran. in a 15-month period, all patients with suspected brucellosis were enrolled. blood specimens were colle ... | 2016 | 27580575 |
| prevalence of the most common virulence-associated genes among brucella melitensis isolates from human blood cultures in hamadan province, west of iran. | brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. despite animal vaccination, brucellosis remains endemic in some areas such as iran, especially in the western iranian province of hamadan. we sought to detect some of the most common virulence-associated genes in brucella isolated from human blood cultures to determine the prevalence of some virulence genes among brucella isolates. fifty-seven isolates were studied from patients with a clinical ... | 2016 | 27582592 |
| brucella melitensis prosthetic joint infection in a traveller returning to the uk from thailand: case report and review of the literature. | brucella spp. prosthetic joint infections are infrequently reported in the literature, particularly in returning travellers, and optimal treatment is unknown. | 2016 | 27591088 |
| immuno-pathophysiological responses of mouse model to experimental infection with brucella melitensis and its lipopolysaccharides via intraperitoneal route. | brucella melitensis is one of the major zoonotic pathogens with significant economic implications worldwide. the pathogenicity is complex and not always well understood. lipopolysaccharide (lps) remains the major virulent factor of b. melitensis and responsible for the mechanism by which the pathogen causes its deleterious effects. in this study, 84 mice of 6-8 weeks old of both sexes were divided equally into 3 groups; namely brucella melitensis infected group, lipopolysaccharide (lps) infected ... | 2016 | 27591112 |
| identification of brucella melitensis rev.1 vaccine-strain genetic markers: towards understanding the molecular mechanism behind virulence attenuation. | brucella melitensis rev.1 is an avirulent strain that is widely used as a live vaccine to control brucellosis in small ruminants. although an assembled draft version of rev.1 genome has been available since 2009, this genome has not been investigated to characterize this important vaccine. in the present work, we used the draft genome of rev.1 to perform a thorough genomic comparison and sequence analysis to identify and characterize the panel of its unique genetic markers. the draft genome of r ... | 2016 | 27595444 |
| human brucellosis in south africa: public health and diagnostic pitfalls. | human brucellosis in south africa (sa) is under-diagnosed and under-reported. this is because many clinicians have little or no experience in managing affected patients, and in part because of the nonspecific and insidious nature of the disease. a case of human brucellosis caused by brucella melitensis in a patient from the western cape province of sa is described, and the resulting exposure of staff members at two medical microbiology laboratories, as well as the public health investigation tha ... | 2016 | 27601111 |
| correction: brucella melitensis methionyl-trna-synthetase (metrs), a potential drug target for brucellosis. | [this corrects the article doi: 10.1371/journal.pone.0160350.]. | 2017 | 27658298 |
| dna-probe-target interaction based detection of brucella melitensis by using surface plasmon resonance. | surface plasmon resonance (spr) immunosensor using 4-mercaptobenzoic acid (4-mba) modified gold (4-mba/au) spr chip was developed first time for the detection of brucella melitensis (b. melitensis) based on the screening of its complementary dna target by using two different newly designed dna probes of is711 gene. herein, interaction between dna probes and target molecule are also investigated and result revealed that the interaction is spontaneous. the kinetics and thermodynamic results derive ... | 2017 | 27665519 |
| susceptibility of select agents to predation by predatory bacteria. | select agents are microorganisms and toxins considered to be exploitable as biological weapons. although infections by many select agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. one group of microorganisms that is showing potential to control drug resistant gram-negative bacteria are the predatory bacteria from the genera bdellovibrio spp. and micavibrio spp. in this study, we have examined the ability of bdello ... | 2015 | 27682124 |
| expression and regulation of the ery operon of brucella melitensis in human trophoblast cells. | brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulates brucella spp. growth. the utilization of erythritol is a characteristic of the genus brucella. the ery operon contains four genes (erya, eryb, eryc and eryd) for the utilization of erythritol, and plays a major role in the survival and multiplication of brucella spp. the objective of the present study was to ... | 2016 | 27698777 |