Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| evolutionary analysis of burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy. | burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 mb) with ∼2,000 genes of putative or unknown function. interactions with potential hosts and environmental factors may induce rapid adaptations in these b. pseudomallei genes, which can be discerned through evolutionary analysis of multiple b. pseudomallei genomes. here we show that several previously uncharacterized b. pseudomallei genes bearing genetic signatures of rapid adaptation (positive s ... | 2013 | 24097950 |
| use of a safe, reproducible, and rapid aerosol delivery method to study infection by burkholderia pseudomallei and burkholderia mallei in mice. | burkholderia pseudomallei, the etiologic agent of melioidosis, is a saprophytic bacterium readily isolated from wet soils of countries bordering the equator. burkholderia mallei is a host-adapted clone of b. pseudomallei that does not persist outside of its equine reservoir and causes the zoonosis glanders, which is endemic in asia, africa, the middle east and south america. infection by these organisms typically occurs via percutaneous inoculation or inhalation of aerosols, and the most common ... | 2013 | 24098563 |
| in vivo manipulation of γ9(+) t cells in the common marmoset (callithrix jacchus) with phosphoantigen and effect on the progression of respiratory melioidosis. | burkholderia pseudomallei is a dangerous human pathogen. phosphoantigens specifically the target primate specific γ9(+)δ2(+) t cells subset and some have been developed as potential immunotherapeutics. previously, we demonstrated that, when stimulated with the phosphoantigen chdmapp, γ9(+)δ2(+) t cells aid in the killing of intracellular b. pseudomallei bacteria. moreover, we found that common marmoset (callithrix jacchus) γ9(+) t cells increase in frequency and respond to the phosphoantigen chd ... | 2013 | 24098670 |
| correlates of immune protection following cutaneous immunization with an attenuated burkholderia pseudomallei vaccine. | infections with the gram-negative bacterium burkholderia pseudomallei (melioidosis) are associated with high mortality, and there is currently no approved vaccine to prevent the development of melioidosis in humans. infected patients also do not develop protective immunity to reinfection, and some individuals will develop chronic, subclinical infections with b. pseudomallei. at present, our understanding of what constitutes effective protective immunity against b. pseudomallei infection remains ... | 2013 | 24101688 |
| pathological findings and diagnostic implications of a rhesus macaque (macaca mulatta) model of aerosol-exposure melioidosis (burkholderia pseudomallei). | aerosolized burkholderia pseudomallei, the causative agent of melioidosis, can infect many species of mammals (including humans), causing rapid, severe pneumonia with high mortality. diagnosis in humans is challenging, as few organisms can be detected in blood or other non-invasive samples. although it cannot be said that the model is established, studies to date indicate that rhesus macaques may represent a good model of human melioidosis. this is supported by the results of this study. the ear ... | 2014 | 24105842 |
| case of paraspinal collection due to burkholderia pseudomallei. | 2013 | 24132451 | |
| burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides. | melioidosis is a potentially fatal disease caused by the saprophytic bacterium burkholderia pseudomallei. resistance to gentamicin is generally a hallmark of b. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. in this study, we determined the prevalence and mechanism of gentamicin susceptibility found in b. pseudomallei isolates from sarawak, malaysian borneo. we performed multilocus sequence typing and antibiotic susceptibility testing on 44 b. pseud ... | 2014 | 24145517 |
| burkholderia mallei and burkholderia pseudomallei cluster 1 type vi secretion system gene expression is negatively regulated by iron and zinc. | burkholderia mallei is a facultative intracellular pathogen that causes glanders in humans and animals. previous studies have demonstrated that the cluster 1 type vi secretion system (t6ss-1) expressed by this organism is essential for virulence in hamsters and is positively regulated by the virag two-component system. recently, we have shown that t6ss-1 gene expression is up-regulated following internalization of this pathogen into phagocytic cells and that this system promotes multinucleated g ... | 2013 | 24146925 |
| glyburide reduces bacterial dissemination in a mouse model of melioidosis. | burkholderia pseudomallei infection (melioidosis) is an important cause of community-acquired gram-negative sepsis in northeast thailand, where it is associated with a ~40% mortality rate despite antimicrobial chemotherapy. we showed in a previous cohort study that patients taking glyburide ( = glibenclamide) prior to admission have lower mortality and attenuated inflammatory responses compared to patients not taking glyburide. we sought to define the mechanism underlying this observation in a m ... | 2013 | 24147174 |
| cytidine derivatives as ispf inhibitors of burkolderia pseudomallei. | published biological data suggest that the methyl erythritol phosphate (mep) pathway, a non-mevalonate isoprenoid biosynthetic pathway, is essential for certain bacteria and other infectious disease organisms. one highly conserved enzyme in the mep pathway is 2c-methyl-d-erythritol 2,4-cyclodiphosphate synthase (ispf). fragment-bound complexes of ispf from burkholderia pseudomallei were used to design and synthesize a series of molecules linking the cytidine moiety to different zinc pocket fragm ... | 2013 | 24157367 |
| in situ hybridization to detect and identify burkholderia pseudomallei in human melioidosis. | burkholderia pseudomallei causes a potentially fatal infection called melioidosis. we have developed a nonfluorescent, colorimetric in situ hybridization assay using a specific probe to target 16s rrna of b. pseudomallei in formalin-fixed, paraffin-embedded infected tissues for diagnostic purposes and to study infectious disease pathology. a 63-base pair dna probe was synthesized and labeled with digoxigenin by pcr. probe specificity was confirmed by blast analysis and by testing on appropriate ... | 2014 | 24186135 |
| colonial morphotypes and biofilm forming ability of burkholderia pseudomallei. | burkholderia pseudomallei the causative agent of melioidosis, is being increasingly recognized as an important cause of morbidity and mortality in south east asia. biofilm formation of b. pseudomallei may be responsible for dormancy, latency and relapse of melioidosis. based on the colonial morphology of the bacteria on b. pseudomallei selective agar medium, seven distinct morphotypes were identified. this study was conducted to assess the in vitro biofilm produced by b. pseudomallei and to inve ... | 2013 | 24189672 |
| sequence-defined transposon mutant library of burkholderia thailandensis. | we constructed a near-saturation transposon mutant library for burkholderia thailandensis, a low-virulence surrogate for the causative agent of melioidosis (burkholderia pseudomallei). a primary set of nearly 42,000 unique mutants (~7.5 mutants/gene) was generated using transposon tn5 derivatives. the strains carry insertions in 87% of the predicted protein-coding genes of the organism, corresponding to nearly all of those nonessential for growth on nutrient agar. to achieve high genome coverage ... | 2013 | 24194535 |
| the in vitro antibiotic susceptibility of malaysian isolates of burkholderia pseudomallei. | acute melioidosis may present as localised or septicaemic infections and can be fatal if left untreated. burkholderia pseudomallei resistant to antibiotics used for the treatment of melioidosis had been reported. the aim of this study was to determine the in vitro antibiotic susceptibility patterns of burkholderia pseudomallei isolated in malaysia to a panel of antibiotics used for the treatment of melioidosis and also to potential alternative antibiotics such as tigecycline, ampicillin/sulbacta ... | 2013 | 24194761 |
| sequence determination of burkholderia pseudomallei strain nctc 13392 colony morphology variants. | burkholderia pseudomallei is a biothreat and the causative agent of melioidosis. there are at least seven known colony morphotypes of b. pseudomallei that appear to have different virulence properties in animal models. we report the genome sequence of b. pseudomallei strain nctc 13392 and the genomic variations of its eight morphotype derivatives. | 2013 | 24201202 |
| cervical abscesses due to co-infection with burkholderia pseudomallei, salmonella enterica serovar stanley and mycobacterium tuberculosis in a patient with diabetes mellitus. | infections due to mycobacterium tuberculosis, burkholderia pseudomallei and non-typhoidal salmonella cause significant morbidity and mortality throughout the world. these intracellular pathogens share some common predisposing factors and clinical features. co-infection with two of these organisms has been reported previously but, to our knowledge, this is the first time that infection with all three has been reported in one person. | 2013 | 24209898 |
| the twin arginine translocation system is essential for aerobic growth and full virulence of burkholderia thailandensis. | the twin arginine translocation (tat) system in bacteria is responsible for transporting folded proteins across the cytoplasmic membrane, and in some bacteria, tat-exported substrates have been linked to virulence. we report here that the tat machinery is present in burkholderia pseudomallei, b. mallei, and b. thailandensis, and we show that the system is essential for aerobic but not anaerobic growth. switching off of the tat system in b. thailandensis grown anaerobically resulted in filamentou ... | 2014 | 24214943 |
| evaluation of aromatic plants and compounds used to fight multidrug resistant infections. | traditional medicine plays a vital role for primary health care in india, where it is widely practiced to treat various ailments. among those obtained from the healers, 78 medicinal plants were scientifically evaluated for antibacterial activity. methanol extract of plants (100 μ g of residue) was tested against the multidrug resistant (mdr) gram-negative and gram-positive bacteria. forty-seven plants showed strong activity against burkholderia pseudomallei (strain tes and khw) and staphylococc ... | 2013 | 24223059 |
| identification of a predicted trimeric autotransporter adhesin required for biofilm formation of burkholderia pseudomallei. | the autotransporters are a large and diverse family of bacterial secreted and outer membrane proteins, which are present in many gram-negative bacterial pathogens and play a role in numerous environmental and virulence-associated interactions. as part of a larger systematic study on the autotransporters of burkholderia pseudomallei, the causative agent of the severe tropical disease melioidosis, we have constructed an insertion mutant in the bpss1439 gene encoding an unstudied predicted trimeric ... | 2013 | 24223950 |
| influence of the molybdenum cofactor biosynthesis on anaerobic respiration, biofilm formation and motility in burkholderia thailandensis. | burkholderia thailandensis is closely related to burkholderia pseudomallei, a bacterial pathogen and the causative agent of melioidosis. b. pseudomallei can survive and persist within a hypoxic environment for up to one year and has been shown to grow anaerobically in the presence of nitrate. currently, little is known about the role of anaerobic respiration in pathogenesis of melioidosis. using b. thailandensis as a model, a library of 1344 transposon mutants was created to identify genes requi ... | 2014 | 24239959 |
| polar lipids of burkholderia pseudomallei induce different host immune responses. | melioidosis is a disease in tropical and subtropical regions of the world that is caused by burkholderia pseudomallei. in endemic regions the disease occurs primarily in humans and goats. in the present study, we used the goat as a model to dissect the polar lipids of b. pseudomallei to identify lipid molecules that could be used for adjuvants/vaccines or as diagnostic tools. we showed that the lipidome of b. pseudomallei and its fractions contain several polar lipids with the capacity to elicit ... | 2013 | 24260378 |
| the burkholderia pseudomallei enoyl-acyl carrier protein reductase fabi1 is essential for in vivo growth and is the target of a novel chemotherapeutic with efficacy. | the bacterial fatty acid biosynthesis pathway is a validated target for the development of novel chemotherapeutics. however, since burkholderia pseudomallei carries genes that encode both fabi and fabv enoyl-acyl carrier protein (acp) reductase homologues, the enoyl-acp reductase that is essential for in vivo growth needs to be defined so that the correct drug target can be chosen for development. accordingly, δfabi1, δfabi2, and δfabv knockout strains were constructed and tested in a mouse mode ... | 2014 | 24277048 |
| trimethoprim-sulfamethoxazole versus trimethoprim-sulfamethoxazole plus doxycycline as oral eradicative treatment for melioidosis (merth): a multicentre, double-blind, non-inferiority, randomised controlled trial. | melioidosis, an infectious disease caused by the gram-negative bacillus burkholderia pseudomallei, is difficult to cure. antimicrobial treatment comprises intravenous drugs for at least 10 days, followed by oral drugs for at least 12 weeks. the standard oral regimen based on trial evidence is trimethoprim-sulfamethoxaxole (tmp-smx) plus doxycycline. this regimen is used in thailand but is associated with side-effects and poor adherence by patients, and tmp-smx alone is recommended in australia. ... | 2014 | 24284287 |
| screen of whole blood responses to flagellin identifies tlr5 variation associated with outcome in melioidosis. | melioidosis is a severe infection caused by the flagellated bacterium burkholderia pseudomallei. the nonsense polymorphism tlr51174c>t is associated with improved outcome in thais with melioidosis. we hypothesized that other tlr5 variants may modulate the host response and determine outcome in melioidosis. we genotyped 12 tlr5 variants selected de novo from the hapmap database and examined the association of each with cytokines induced by flagellin stimulation of whole blood from healthy thai su ... | 2014 | 24285178 |
| glibenclamide reduces pro-inflammatory cytokine production by neutrophils of diabetes patients in response to bacterial infection. | type 2 diabetes mellitus is a major risk factor for melioidosis, which is caused by burkholderia pseudomallei. our previous study has shown that polymorphonuclear neutrophils (pmns) from diabetic subjects exhibited decreased functions in response to b. pseudomallei. here we investigated the mechanisms regulating cytokine secretion of pmns from diabetic patients which might contribute to patient susceptibility to bacterial infections. purified pmns from diabetic patients who had been treated with ... | 2013 | 24285369 |
| the role of nod2 in murine and human melioidosis. | nucleotide-binding oligomerization domain 2 (nod2) is a cytosolic pathogen recognition receptor that regulates susceptibility to a variety of infections and chronic diseases. burkholderia pseudomallei, a facultative intracellular bacterium, causes the tropical infection melioidosis. we hypothesized that nod2 may participate in host defense in melioidosis. we performed a series of in vitro assays and in vivo experiments and analyzed the association of human genetic variation with infection to del ... | 2014 | 24298015 |
| survey of innate immune responses to burkholderia pseudomallei in human blood identifies a central role for lipopolysaccharide. | b. pseudomallei is a gram-negative bacterium that causes the tropical infection melioidosis. in northeast thailand, mortality from melioidosis approaches 40%. as exemplified by the lipopolysaccharide-toll-like receptor 4 interaction, innate immune responses to invading bacteria are precipitated by activation of host pathogen recognition receptors by pathogen associated molecular patterns. human melioidosis is characterized by up-regulation of pathogen recognition receptors and pro-inflammatory c ... | 2013 | 24303060 |
| natural infection of burkholderia pseudomallei in an imported pigtail macaque (macaca nemestrina) and management of the exposed colony. | identification of the select agent burkholderia pseudomallei in macaques imported into the united states is rare. a purpose-bred, 4.5-y-old pigtail macaque (macaca nemestrina) imported from southeast asia was received from a commercial vendor at our facility in march 2012. after the initial acclimation period of 5 to 7 d, physical examination of the macaque revealed a subcutaneous abscess that surrounded the right stifle joint. the wound was treated and resolved over 3 mo. in august 2012, 2 mo a ... | 2013 | 24326230 |
| a case of mycotic aneurysm due to burkholderia pseudomallei. | burkholderia pseudomallei is an free-living gram-negative bacterium causing melioidosis and is endemic in southeast asia. a 56-year-old diabetic construction worker with a 1-month history of abdominal pain and 1-day history of high-grade fever was found to have a left non-dissecting infrarenal mycotic aortic aneurysm by abdominal computerized tomography scan. bacteriological examination of his blood yielded burkholderia pseudomallei. the patient was treated with right axillo-bifemoral bypass wit ... | 2013 | 24353601 |
| a structural biology approach enables the development of antimicrobials targeting bacterial immunophilins. | macrophage infectivity potentiators (mips) are immunophilin proteins and essential virulence factors for a range of pathogenic organisms. we applied a structural biology approach to characterize a mip from burkholderia pseudomallei (bpml1), the causative agent of melioidosis. crystal structure and nuclear magnetic resonance analyses of bpml1 in complex with known macrocyclics and other derivatives led to the identification of a key chemical scaffold. this scaffold possesses inhibitory potency fo ... | 2014 | 24366729 |
| activation of nadph oxidase is essential, but not sufficient, in controlling intracellular multiplication of burkholderia pseudomallei in primary human monocytes. | burkholderia pseudomallei is a gram-negative intracellular bacterium and the causative agent of melioidosis. innate immune mechanisms against this pathogen, which might contribute to outcomes of melioidosis, are little known. we demonstrated here that b. pseudomallei could activate nadph oxidase in primary human monocytes as judged by production of reactive oxygen species (ros) and p40(phox) phosphorylation after infection. however, as similar to other intracellular bacteria, this bacterium was ... | 2014 | 24376210 |
| discovery of inhibitors of burkholderia pseudomallei methionine aminopeptidase with antibacterial activity. | evaluation of a series of metap inhibitors in an in vitro enzyme activity assay led to the first identification of potent molecules that show significant growth inhibition against burkholderia pseudomallei. nitroxoline analogs show excellent inhibition potency in the bpmetap1 enzyme activity assay with the lowest ic50 of 30 nm, and inhibit the growth of b. pseudomallei and b. thailandensis at concentrations ≥ 31 μm. | 2013 | 24376907 |
| substituted diphenyl ethers as a novel chemotherapeutic platform against burkholderia pseudomallei. | identification of a novel class of anti-burkholderia compounds is key in addressing antimicrobial resistance to current therapies as well as naturally occurring resistance. the fabi enoyl-acp reductase in burkholderia is an underexploited target that presents an opportunity for development of a new class of inhibitors. a library of substituted diphenyl ethers was used to identify fabi1-specific inhibitors for assessment in burkholderia pseudomallei ex vivo and murine efficacy models. active fabi ... | 2014 | 24379198 |
| the role of short-chain dehydrogenase/oxidoreductase, induced by salt stress, on host interaction of b. pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a frequently occurring disease in northeastern thailand, where soil and water high in salt content are common. using microarray analysis, we previously showed that b. pseudomallei up-regulated a short-chain dehydrogenase/oxidoreductase (sdo) under salt stress. however, the importance of sdo in b. pseudomallei infection is unknown. this study aimed to explore the function of b. pseudomallei sdo, and to investigate its role in intera ... | 2014 | 24382268 |
| atypical morphological characteristics and surface antigen expression of burkholderia pseudomallei in naturally infected human synovial tissues. | abstract burkholderia pseudomallei is the causative agent of melioidosis, a disease that frequently runs a protracted course and is very difficult to eradicate. the mechanisms that this organism uses to escape from host defense mechanisms and antibiotics are not well understood. the aim of the study was to compare the morphological characteristics and surface antigen expression of b. pseudomallei in naturally infected human synovial tissues with the characteristics of bacteria grown in culture m ... | 2000 | 24383589 |
| a multiplex nested pcr for the simultaneous detection of salmonella typhi, mycobacterium tuberculosis, and burkholderia pseudomallei in patients with pyrexia of unknown origin (puo) in vellore, south india. | salmonella typhi, mycobacterium tuberculosis, and burkholderia pseudomallei are among the most important monocyte-tropic bacterial agents causing pyrexia of unknown origin (puo), with a significant number of endemic infections in both south and southeast asian regions. these infections pose a major risk to travelers to these regions as well. | 2014 | 24385404 |
| common tlr1 genetic variation is not associated with death from melioidosis, a common cause of sepsis in rural thailand. | melioidosis, infection caused by the gram-negative bacterium burkholderia pseudomallei, is a common cause of sepsis in northeast thailand. in white north americans, common functional genetic variation in tlr1 is associated with organ failure and death from sepsis. we hypothesized that tlr1 variants would be associated with outcomes in thais with melioidosis. we collated the global frequencies of three tlr1 variants that are common in white north american populations: rs5743551 (-7202a/g), rs4833 ... | 2014 | 24392083 |
| mice lacking the lectin-like domain of thrombomodulin are protected against melioidosis. | thrombomodulin is a multidomain receptor primarily expressed by vascular endothelium. the lectin-like domain of thrombomodulin has anti-inflammatory properties. in this study, we investigated the role of the thrombomodulin lectin-like domain in the host response to gram-negative sepsis caused by burkholderia pseudomallei, a "tier 1" biothreat agent and the causative agent of melioidosis, a common form of community-acquired sepsis in southeast asia. | 2014 | 24399241 |
| osteomyelitis and septic arthritis from infection with burkholderia pseudomallei: a 20-year prospective melioidosis study from northern australia. | the gram-negative organism, burkholderia pseudomallei, is responsible for the disease melioidosis. septic arthritis and osteomyelitis due to b. pseudomallei are rare but recognised presentations of the disease. | 2013 | 24403756 |
| the type vi secretion system spike protein vgrg5 mediates membrane fusion during intercellular spread by pseudomallei group burkholderia species. | pseudomallei group burkholderia species are facultative intracellular parasites that spread efficiently from cell to cell by a mechanism involving the fusion of adjacent cell membranes. intercellular fusion requires the function of the cluster 5 type vi secretion system (t6ss-5) and its associated valine-glycine repeat protein, vgrg5. here we show that vgrg5 alleles are conserved and functionally interchangeable between burkholderia pseudomallei and its relatives b. mallei, b. oklahomensis, and ... | 2014 | 24421040 |
| reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents. | the need for a rapid detection and characterization of biowarfare (bw) agents cannot be over emphasized. with diverse array of potential bw pathogen available presently, rapid identification of the pathogen is crucial, so that specific therapy and control measures can be initiated. we have developed a multiplex polymerase chain reaction based reverse line blot macroarray to simultaneously detect four pathogens of bw importance viz. bacillus anthracis, yersinia pestis, brucella melitensis and bur ... | 2013 | 24426077 |
| uncommon clinical presentations of melioidosis in children: 2 cases with sore throat and 1 case with urticarial rash. | common clinical presentations of melioidosis in children include suppurative parotitis, lymphadenitis, skin infection and septicemia with pneumonia. here we describe three cases with uncommon presentations of melioidosis seen among children attending a university hospital in northeastern thailand. two patients presented with pharyngitis and subsequently developed cervical lymphadenitis. another patient presented with high fever and generalized urticarial rash. a pharyngeal culture in each of the ... | 2013 | 24437321 |
| chronic suppurative joint effusion due to burkholderia pseudomallei: a case report. | burkholderia pseudomallei, a gram-negative bacillus is the causative agent of melioidosis, a glanders-like disease, primarily a disease of animals. melioidosis has been only a rare and sporadic disease in humans outside its endemic region. currently, diagnosis of b. pseudomallei in the clinical laboratory is very difficult, owing to low awareness of physicians to the nonspecific clinical manifestations, lack of responsiveness among microbiologists outside endemic areas, identification systems in ... | 2014 | 24441249 |
| alteration of the phenotypic and pathogenic patterns of burkholderia pseudomallei that persist in a soil environment. | melioidosis is caused by the soil-borne pathogen burkholderia pseudomallei. to investigate whether the distinct phenotypic and virulent characteristics result from environmental adaptations in the soil or from the host body, two pairs of isogenic strains were generated by passages in soil or mice. after cultivation in soil, the levels of 3-hydroxytetradecanoic acid, biofilm formation, flagellar expression, and ultrastructure were altered in the bacteria. uniformly fatal melioidosis developed as ... | 2014 | 24445207 |
| physicochemical factors affecting the growth of burkholderia pseudomallei in soil microcosm. | burkholderia pseudomallei causes melioidosis, the third most common cause of death from infectious diseases in northeast thailand. four physicochemical factors were set so that their values covered the range of the northeast, which is an endemic area. the soil ph was set at ph 4-10, soil salinity was 0.0-5.0% nacl, total iron was 50-150 mg/kg soil, and carbon to nitrogen ratio (c/n) was 10:1 to 40:1. the experiments were carried out at 37°c, and soil moisture was maintained for 7 days. the numbe ... | 2014 | 24445210 |
| burkholderia pseudomallei isolates in 2 pet iguanas, california, usa. | burkholderia pseudomallei, the causative agent of melioidosis, was isolated from abscesses of 2 pet green iguanas in california, usa. the international trade in iguanas may contribute to importation of this pathogen into countries where it is not endemic and put persons exposed to these animals at risk for infection. | 2014 | 24447394 |
| melioidosis caused by burkholderia pseudomallei in drinking water, thailand, 2012. | we identified 10 patients in thailand with culture-confirmed melioidosis who had burkholderia pseudomallei isolated from their drinking water. the multilocus sequence type of b. pseudomallei from clinical specimens and water samples were identical for 2 patients. this finding suggests that drinking water is a preventable source of b. pseudomallei infection. | 2014 | 24447771 |
| efficient inactivation of burkholderia pseudomallei or francisella tularensis in infected cells for safe removal from biosafety level 3 containment laboratories. | working with infectious agents that require bsl-3 level containment agents offers many challenges for researchers. bsl-3 containment laboratories are usually not equipped with expensive specialty equipment that is needed for studies such as flow cytometric analysis, microscopy, and proteomic analyses. therefore, for most researchers that are working with bsl-3 level infectious agents, removal of samples from bsl-3 laboratories for these types of studies is necessary, and methods for complete and ... | 2014 | 24449562 |
| vgrg-5 is a burkholderia type vi secretion system-exported protein required for multinucleated giant cell formation and virulence. | the type vi secretion system (t6ss) has emerged as a critical virulence factor for the group of closely related burkholderia spp. that includes burkholderia pseudomallei, b. mallei, and b. thailandensis. while the genomes of these bacteria, referred to as the bptm group, appear to encode several t6sss, we and others have shown that one of these, type vi secretion system 5 (t6ss-5), is required for virulence in mammalian infection models. despite its pivotal role in the pathogenesis of the bptm g ... | 2014 | 24452686 |
| melioidosis of salivary glands with coexisting diabetes: management of a difficult case. | melioidosis is an infectious disease caused by the gram-negative bacillus burkholderia pseudomallei. melioidosis is endemic in southeast asia and northern australia. an important risk factor for the development of melioidosis is the presence of diabetes mellitus, which may increase the relative risk of infection by as much as 100-fold. we report a rare case of melioidosis of the parotid and submandibular gland with coexisting diabetes. this was successfully managed conservatively with intravenou ... | 2014 | 24452899 |
| population-sequencing as a biomarker of burkholderia mallei and burkholderia pseudomallei evolution through microbial forensic analysis. | large-scale genomics projects are identifying biomarkers to detect human disease. b. pseudomallei and b. mallei are two closely related select agents that cause melioidosis and glanders. accurate characterization of metagenomic samples is dependent on accurate measurements of genetic variation between isolates with resolution down to strain level. often single biomarker sensitivity is augmented by use of multiple or panels of biomarkers. in parallel with single biomarker validation, advances in ... | 2013 | 24455204 |
| the serine protease inhibitor ecotin is required for full virulence of burkholderia pseudomallei. | burkholderia pseudomallei is a gram negative soil saprophyte that causes the disease melioidosis where clinical symptoms can vary from localised infection to pneumonia and septic shock. ecotin is a potent periplasmic serine protease inhibitor first identified in escherichia coli. ecotin, although present in only a small subset of genera, can inhibit a broad range of serine proteases including those typically associated with the innate immune system such as neutrophil elastase and cathepsin g. an ... | 2014 | 24462575 |
| global analysis of the burkholderia thailandensis quorum sensing-controlled regulon. | burkholderia thailandensis contains three acyl-homoserine lactone quorum sensing circuits and has two additional luxr homologs. to identify b. thailandensis quorum sensing-controlled genes, we carried out transcriptome sequencing (rna-seq) analyses of quorum sensing mutants and their parent. the analyses were grounded in the fact that we identified genes coding for factors shown previously to be regulated by quorum sensing among a larger set of quorum-controlled genes. we also found that genes c ... | 2014 | 24464461 |
| recurrent melioidosis in the darwin prospective melioidosis study: improving therapies mean that relapse cases are now rare. | the darwin prospective melioidosis study has documented 785 melioidosis cases over 23 years. recurrent melioidosis occurred in 39/679 (5.7%) patients surviving initial infection; 29 patients suffered relapse of the original infection, and 10 presented with a new burkholderia pseudomallei infection. with improved therapy, relapse has become rare in recent years. | 2014 | 24478504 |
| structural characterization of polysaccharides expressed by burkholderia oklahomensis e0147. | burkholderia oklahomensis e0147 is a us isolated bacterium believed to express a similar o-antigen to type a structure of the highly pathogenic species, burkholderia pseudomallei. both species are genetically closely related. lipopolysaccharide was collected from e0147 and structurally characterized to test this hypothesis. glycosyl composition and linkage analyses in conjunction with 1d and 2d (1)h and (13)c nmr spectroscopy showed that the o-antigen was a repeating disaccharide with the follow ... | 2014 | 24491843 |
| mining locus tags in pubmed central to improve microbial gene annotation. | the scientific literature contains millions of microbial gene identifiers within the full text and tables, but these annotations rarely get incorporated into public sequence databases. we propose to utilize the open access (oa) subset of pubmed central (pmc) as a gene annotation database and have developed an r package called pmcxml to automatically mine and extract locus tags from full text, tables and supplements. | 2014 | 24499370 |
| the hica toxin from burkholderia pseudomallei has a role in persister cell formation. | ta (toxin-antitoxin) systems are widely distributed amongst bacteria and are associated with the formation of antibiotic tolerant (persister) cells that may have involvement in chronic and recurrent disease. we show that overexpression of the burkholderia pseudomallei hica toxin causes growth arrest and increases the number of persister cells tolerant to ciprofloxacin or ceftazidime. furthermore, our data show that persistence towards ciprofloxacin or ceftazidime can be differentially modulated ... | 2014 | 24502667 |
| in vitro activity of ceftaroline against burkholderia pseudomallei. | 2014 | 24505090 | |
| colonization or spontaneous resolution: expanding the role for burkholderia pseudomallei. | a 19-year-old asian indian female presented with productive cough since the past one month and low grade fever since the past two weeks. she was diagnosed with pulmonary tuberculosis and treated with antitubercular drugs. subsequently, delayed cultures of bronchoalveolar lavage fluid grew burkholderia pseudomallei (b. pseudomallei). on follow up the patient reported significant subjective improvement and esr progressively returned to normal. in summary, this case report raises two distinct and e ... | 2014 | 24507650 |
| whitmore's disease: an uncommon urological presentation. | the incidence of prostatic abscesses has much decreased in the antibiotic era. we present an uncommon cause of prostatic abscess secondary to melioidosis, also known as whitmore's disease or pseudoglanders. the disease is endemic in south east asia and australia. although india is considered endemic for burkholderia pseudomallei, the causative organism of melioidosis, not many cases have been reported. most of the reported cases from india are from the south-west coastal regions of kerala and ka ... | 2014 | 24518392 |
| genome-wide saturation mutagenesis of burkholderia pseudomallei k96243 predicts essential genes and novel targets for antimicrobial development. | burkholderia pseudomallei is the causative agent of melioidosis, an often fatal infectious disease for which there is no vaccine. b. pseudomallei is listed as a tier 1 select agent, and as current therapeutic options are limited due to its natural resistance to most antibiotics, the development of new antimicrobial therapies is imperative. to identify drug targets and better understand the complex b. pseudomallei genome, we sought a genome-wide approach to identify lethal gene targets. as b. pse ... | 2014 | 24520057 |
| wound healing activity and mechanisms of action of an antibacterial protein from the venom of the eastern diamondback rattlesnake (crotalus adamanteus). | basic phospholipase a2 was identified from the venom of the eastern diamondback rattlesnake. the crotalus adamanteus toxin-ii (catx-ii) induced bactericidal effects (7.8 µg/ml) on staphylococcus aureus, while on burkholderia pseudomallei (khw), and enterobacter aerogenes were killed at 15.6 µg/ml. catx-ii caused pore formation and membrane damaging effects on the bacterial cell wall. catx-ii was not cytotoxic on lung (mrc-5), skin fibroblast (hepk) cells and in mice. catx-ii-treated mice showed ... | 2014 | 24551028 |
| pathogenesis of percutaneous infection of goats with burkholderia pseudomallei: clinical, pathologic, and immunological responses in chronic melioidosis. | melioidosis is a severe suppurative to granulomatous infection caused by burkholderia pseudomallei. the disease is endemic to south-east asia and northern australasia and is also of interest as a potential biological weapon. natural infection can occur by percutaneous inoculation, inhalation or ingestion, but the relative importance of each route is unknown. experimental infection models using mice have shown inhalation to be the most lethal route of exposure, but few studies have examined the p ... | 2014 | 24571408 |
| thoracic melioidosis: a diagnostic dilemma. | the diagnosis of melioidosis can be difficult, and it is frequently described as "the great mimicker". we report a case of thoracic melioidosis presenting as a mediastinal mass with impending superior vena cava obstruction. with the presumptive diagnosis of mediastinal tumor, the patient underwent surgery for tissue sampling, and a purulent collection was found. the clinical syndromes of melioidosis and the diagnostic dilemma are discussed. | 2015 | 24585290 |
| bpss1504, a cluster 1 type vi secretion gene, is involved in intracellular survival and virulence of burkholderia pseudomallei. | burkholderia pseudomallei is a gram-negative rod and the causative agent of melioidosis, an emerging infectious disease of tropical and subtropical areas worldwide. b. pseudomallei harbors a remarkable number of virulence factors, including six type vi secretion systems (t6ss). using our previously described plaque assay screening system, we identified a b. pseudomallei transposon mutant defective in the bpss1504 gene that showed reduced plaque formation. the bpss1504 locus is encoded within t6s ... | 2014 | 24595140 |
| role of burkholderia pseudomallei biofilm formation and lipopolysaccharide in relapse of melioidosis. | we examined whether quantitative biofilm formation and/or lipopolysaccharide type of burkholderia pseudomallei was associated with relapsing melioidosis. we devised a 1:4 nested case-control study in which both cases and controls were drawn from a cohort of patients with primary melioidosis. paired isolates from 80 patients with relapse and single isolates from 184 patients without relapse were tested. relapse was associated with biofilm formation of the primary infecting isolate (conditional or ... | 2014 | 24602145 |
| comparative burkholderia pseudomallei natural history virulence studies using an aerosol murine model of infection. | melioidosis is an endemic disease caused by the bacterium burkholderia pseudomallei. concerns exist regarding b. pseudomallei use as a potential bio-threat agent causing persistent infections and typically manifesting as severe pneumonia capable of causing fatal bacteremia. development of suitable therapeutics against melioidosis is complicated due to high degree of genetic and phenotypic variability among b. pseudomallei isolates and lack of data establishing commonly accepted strains for compa ... | 2014 | 24603493 |
| acute pulmonary melioidosis in a child: a case report and review of literature. | melioidosis is a rare potentially fatal infection caused by the bacterium burkholderia pseudomallei, which is endemic in southeast asia and northern australia and an emerging pathogen in india. it is a largely under-diagnosed disease and an imitator of tuberculosis, both clinically and radiologically. | 2013 | 24604934 |
| septicemic melioidosis: a case report and literature review. | this report was about a 22-year-old male patient who died from acute and severe septicemic melioidosis. he was initially misdiagnosed as having pneumonia in a local hospital in hainan but transferred to guangzhou red cross hospital after ineffective treatment and detection of burkholderia pseudomallei in his blood sample. unfortunately, right diagnosis and antibiotic treatment of melioidosis had been delayed so that his condition deteriorated rapidly out of control. the victim's death highlights ... | 2014 | 24605237 |
| treatment and prophylaxis of melioidosis. | melioidosis, infection with burkholderia pseudomallei, is being recognised with increasing frequency and is probably more common than currently appreciated. treatment recommendations are based on a series of clinical trials conducted in thailand over the past 25 years. treatment is usually divided into two phases: in the first, or acute phase, parenteral drugs are given for ≥10 days with the aim of preventing death from overwhelming sepsis; in the second, or eradication phase, oral drugs are giv ... | 2014 | 24613038 |
| functional characterization and evaluation of in vitro protective efficacy of murine monoclonal antibodies burk24 and burk37 against burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis has been recognized by cdc as a category b select agent. although substantial efforts have been made for development of vaccine molecules against the pathogen, significant hurdles still remain. with no licensed vaccines available and high relapse rate of the disease, there is a pressing need for development of alternate protection strategies. antibody-mediated passive protection is promising in this regard and our primary interest was ... | 2014 | 24614539 |
| variable virulence factors in burkholderia pseudomallei (melioidosis) associated with human disease. | burkholderia pseudomallei is a gram-negative environmental bacterium that causes melioidosis, a potentially life-threatening infectious disease affecting mammals, including humans. melioidosis symptoms are both protean and diverse, ranging from mild, localized skin infections to more severe and often fatal presentations including pneumonia, septic shock with multiple internal abscesses and occasionally neurological involvement. several ubiquitous virulence determinants in b. pseudomallei have al ... | 2014 | 24618705 |
| etiologies and resistance profiles of bacterial community-acquired pneumonia in cambodian and neighboring countries' health care settings: a systematic review (1995 to 2012). | community-acquired pneumonia (cap) is one of the most important causes of morbidity and mortality worldwide. etiological data for cambodia is scarce. we aimed to describe the main etiological agents causing cap, and their resistance patterns in cambodia and the greater mekong region. | 2014 | 24626053 |
| caspase-1-dependent and -independent cell death pathways in burkholderia pseudomallei infection of macrophages. | the cytosolic pathogen burkholderia pseudomallei and causative agent of melioidosis has been shown to regulate il-1β and il-18 production through nod-like receptor nlrp3 and pyroptosis via nlrc4. downstream signalling pathways of those receptors and other cell death mechanisms induced during b. pseudomallei infection have not been addressed so far in detail. furthermore, the role of b. pseudomallei factors in inflammasome activation is still ill defined. in the present study we show that caspase ... | 2014 | 24626296 |
| [melioidosis, first four cases in iceland]. | we report the first four cases of melioidosis treated in iceland and review the literature. melioidosis is caused by the saprophytic gram negative bacteria burkholderia pseudomallei. most disease occurs in residents of southeast-asia and north-australia. the most common presentation of melioidosis is pneumonia but as these cases demonstrate the infection has protean manifestations and b. pseudomallei can infect nearly every organ. it is important to notify the laboratory of the possibilty of mel ... | 2014 | 24639431 |
| development of a prototype lateral flow immunoassay (lfi) for the rapid diagnosis of melioidosis. | burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. isolation of b. pseudomallei from clinical samples is the "gold standard" for the diagnosis of melioidosis; results can take 3-7 days to produce. alternatively, antibody-based tests have low specificity due to a high percentage of seropositive individuals in endemic areas. there is a clear need to develop a rapid point-of-care antigen detection assay for the diagnosis of melioidosis. previously, we emp ... | 2014 | 24651568 |
| burkholderia pseudomallei type g in western hemisphere. | burkholderia pseudomallei isolates from the western hemisphere are difficult to differentiate from those from regions in which melioidosis is traditionally endemic. we used internal transcribed spacer typing to determine that b. pseudomallei isolates from the western hemisphere are consistently type g. knowledge of this relationship might be useful for epidemiologic investigations. | 2014 | 24655932 |
| distribution of burkholderia pseudomallei in northern australia, a land of diversity. | burkholderia pseudomallei is a gram-negative soil bacillus that is the etiological agent of melioidosis and a biothreat agent. little is known about the biogeography of this bacterium in australia, despite its hyperendemicity in the northern region of this continent. the population structure of 953 australian b. pseudomallei strains representing 779 and 174 isolates of clinical and environmental origins, respectively, was analyzed using multilocus sequence typing (mlst). bayesian population stru ... | 2014 | 24657869 |
| subcutaneous surprise. | melioidosis is a zoonosis caused by the accidental pathogen burkholderia pseudomallei, which is endemic in southeast asia and northern australia. the mortality of melioidosis is 20-50% even with treatment. suppurative lymphadenitis caused by melioidosis has been rarely encountered by clinicians practicing in endemic areas. in the majority of previously described patients, the infected lymph nodes were in the head and neck region, except for four patients who presented with unilateral, inguinal l ... | 2014 | 24669344 |
| a burkholderia pseudomallei outer membrane vesicle vaccine provides protection against lethal sepsis. | the environmental gram-negative encapsulated bacillus burkholderia pseudomallei is the causative agent of melioidosis, a disease associated with high morbidity and mortality rates in areas of southeast asia and northern australia in which the disease is endemic. b. pseudomallei is also classified as a tier i select agent due to the high level of lethality of the bacterium and its innate resistance to antibiotics, as well as the lack of an effective vaccine. gram-negative bacteria, including b. p ... | 2014 | 24671550 |
| evaluation of the remel rapid nf plus rapid biochemical method for identification of burkholderia pseudomallei. | typical methods for the identification of burkholderia pseudomallei colonies produce results in 18 h. the remel rapid nf plus kit produces results in 4 h. we used the kit for 190 stored b. pseudomallei isolates and correctly identified 189 of them. this kit produces consistent results for known b. pseudomallei isolates. | 2014 | 24671779 |
| reactivation of latent melioidosis presenting with acute pyelonephritis and bacteremia. | we report an interesting case involving an 81-year-old male patient who presented to our hospital with sepsis secondary to pyelonephritis and bacteremia. blood cultures were positive for a gram-negative bacillus, burkholderia pseudomallei. his risk factors were diabetes, chronic obstructive pulmonary disease including travel to an endemic area. the patient received antibiotic therapy in accordance with the national guidelines. this is the first report of reactivation of latent melioidosis in the ... | 2014 | 24678468 |
| genetic control of weight loss during pneumonic burkholderia pseudomallei infection. | burkholderia pseudomallei (bp) is the causal agent of a high-morbidity/mortality disease syndrome known as melioidosis. this syndrome can range from acute fulminate disease to chronic, local, and disseminated infections that are often difficult to treat because bp exhibits resistance to many antibiotics. bp is a prime candidate for use in biologic warfare/terrorism and is classified as a tier-1 select agent by hhs and aphis. it is known that inbred mouse strains display a range of susceptibility ... | 2014 | 24687986 |
| bone marrow-derived macrophages from balb/c and c57bl/6 mice fundamentally differ in their respiratory chain complex proteins, lysosomal enzymes and components of antioxidant stress systems. | macrophages are essential components of the innate immune system and crucial for pathogen elimination in early stages of infection. we previously observed that bone marrow-derived macrophages (bmms) from c57bl/6 mice exhibited increased killing activity against burkholderia pseudomallei compared to bmms from balb/c mice. this effect was particularly pronounced when cells were treated with ifn-γ. to unravel mechanisms that could explain these distinct bactericidal effects, a comparative combined ... | 2014 | 24704164 |
| evaluation of a latex agglutination assay for the identification of burkholderia pseudomallei and burkholderia mallei. | cases of melioidosis and glanders are rare in the united states, but the etiologic agents of each disease (burkholderia pseudomallei and burkholderia mallei, respectively) are classified as tier 1 select agents because of concerns about their potential use as bioterrorism agents. a rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. our laboratory has further evaluated a latex agglutination assay for its ability to identify b. pseudomallei and b. mall ... | 2014 | 24710616 |
| splenic abscess due to chronic melioidosis in a patient previously misdiagnosed as tuberculosis. | melioidosis is endemic in southeast asia and northern australia. sporadic cases have been reported from many parts of the world where it has an epidemic potential with high-rate fatality cases. in non-endemic areas, melioidosis may be misdiagnosed with common diseases and this may prove fatal. sporadic cases of melioidosis are mistaken for tuberculosis in india. we report a case of splenic abscess due to chronic melioidosis who was earlier misdiagnosed as tuberculosis and underwent anti-tubercul ... | 2013 | 24714690 |
| two fatal cases of melioidosis on the thai-myanmar border. | melioidosis is endemic in areas of southeast asia, however, there are no published reports from the thai-myanmar border. we report the first two documented cases of fatal melioidosis in this region. this is of great public health importance and highlights the need to both increase clinical awareness of melioidosis on the thai-myanmar border, and to assess the true burden of disease in the area through improved case detection and burkholderia pseudomallei prevalence studies. | 2014 | 24715973 |
| redefining the pf06864 pfam family based on burkholderia pseudomallei pilo2(bp) s-sad crystal structure. | type iv pili are surface-exposed filaments and bacterial virulence factors, represented by the tfpa and tfpb types, which assemble via specific machineries. the tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. here we focus on pilo2(bp), a protein component of the tfpb r64 thin pilus variant assembly machinery from the pathogen burkholderia pseudomallei. pilo2(bp) belongs to the pf06864 pfam family, for which an improved definition is presen ... | 2014 | 24728008 |
| characterization of an autotransporter adhesin protein shared by burkholderia mallei and burkholderia pseudomallei. | autotransporters form a large family of outer membrane proteins specifying diverse biological traits of gram-negative bacteria. in this study, we report the identification and characterization of a novel autotransporter gene product of burkholderia mallei (locus tag bma1027 in strain atcc 23344). | 2014 | 24731253 |
| burkholderia pseudomallei penetrates the brain via destruction of the olfactory and trigeminal nerves: implications for the pathogenesis of neurological melioidosis. | abstract melioidosis is a potentially fatal disease that is endemic to tropical northern australia and southeast asia, with a mortality rate of 14 to 50%. the bacterium burkholderia pseudomallei is the causative agent which infects numerous parts of the human body, including the brain, which results in the neurological manifestation of melioidosis. the olfactory nerve constitutes a direct conduit from the nasal cavity into the brain, and we have previously reported that b. pseudomallei can colon ... | 2014 | 24736221 |
| enhanced intracellular survival and epithelial cell adherence abilities of burkholderia pseudomallei morphotypes are dependent on differential expression of virulence-associated proteins during mid-logarithmic growth phase. | colony morphology variation is a characteristic of burkholderia pseudomallei primary clinical isolates, associated with variations in expression of virulence factors. here, we performed comparative investigations on adhesion, invasion, plaque-forming abilities and protein profiles of b. pseudomallei wild-type (wt) and a small colony variant (scv). the percentage of scv adherence to a549 cells was significantly higher (2.73%) than wt (1.91%). in contrast, wt was significantly more efficient (0.63 ... | 2014 | 24742602 |
| a high-content imaging assay for the quantification of the burkholderia pseudomallei induced multinucleated giant cell (mngc) phenotype in murine macrophages. | burkholderia pseudomallei (bp), a gram-negative, motile, facultative intracellular bacterium is the causative agent of melioidosis in humans and animals. the bp genome encodes a repertoire of virulence factors, including the cluster 3 type iii secretion system (t3ss-3), the cluster 1 type vi secretion system (t6ss-1), and the intracellular motility protein bima, that enable the pathogen to invade both phagocytic and non-phagocytic cells. a unique hallmark of bp infection both in vitro and in viv ... | 2014 | 24750902 |
| growth, motility and resistance to oxidative stress of the melioidosis pathogen burkholderia pseudomallei are enhanced by epinephrine. | burkholderia pseudomallei causes melioidosis, a severe invasive disease endemic in south-east asia and northern australia. bacterial pathogens of several genera have been reported to be able to sense and respond to the stress-related catecholamine hormone epinephrine. here, we report that epinephrine induces growth of b. pseudomallei in minimal serum-rich medium and heat-inactivated whole human serum and enhances bacterial motility, transcription of flagellar genes and flagellin synthesis. the e ... | 2014 | 24753312 |
| sulphonylurea usage in melioidosis is associated with severe disease and suppressed immune response. | melioidosis is a problem in the developing tropical regions of southeast asia and northern australia where the the gram negative saprophytic bacillus burkholderia pseudomallei is endemic with the risk of fulminant septicaemia. while diabetes mellitus is a well-established risk factor for melioidiosis, little is known if specific hypoglycemic agents may differentially influence the susceptibility and clinical course of infection with b. pseudomallei (bp). | 2014 | 24762472 |
| melioidosis :an emerging infection in india. | melioidosis caused by the gram-negative bacterium burkholderia pseudomallei is endemic in southeast asia but may be under-diagnosed and under-reported in the indian subcontinent. this study was undertaken to analyse the clinical presentation and epidemiological risk factors for melioidosis in india. | 2013 | 24772696 |
| the bacterial aetiology of adult community-acquired pneumonia in asia: a systematic review. | community-acquired pneumonia (cap) is a major cause of adult mortality in asia. appropriate empirical treatment depends on knowledge of the pathogens commonly responsible. however, assessing the aetiological significance of identified organisms is often difficult, particularly with sputum isolates that might represent contamination with oropharyngeal flora. | 2014 | 24781376 |
| clinical features of diabetes mellitus cases complicated by burkholderia pseudomallei septicemia. | the aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. the clinical characteristics, diagnosis, treatment, and prognosis of 39 diabetes mellitus patients with b. pseudomallei septicemia were retrospectively analyzed. farmers, fishermen and workers were found to be high-risk groups. the clinical manifestations of patients were diverse without specific features, bu ... | 2014 | 24782168 |
| intramolecular aglycon delivery enables the synthesis of 6-deoxy-β-d-manno-heptosides as fragments of burkholderia pseudomallei and burkholderia mallei capsular polysaccharide. | burkholderia pseudomallei and burkholderia mallei are potential bioterrorism agents. they express the same capsular polysaccharide (cps), a homopolymer featuring an unusual [→3)-2-o-acetyl-6-deoxy-β-d-manno-heptopyranosyl-(1→] as the repeating unit. this cps is known to be one of the main targets of the adaptive immune response in humans and therefore represents a crucial subunit candidate for vaccine development. herein, the stereoselective synthesis of mono- and disaccharidic fragments of the ... | 2014 | 24786555 |
| porin involvement in cephalosporin and carbapenem resistance of burkholderia pseudomallei. | burkholderia pseudomallei (bps) is a gram-negative bacterium that causes frequently lethal melioidosis, with a particularly high prevalence in the north and northeast of thailand. bps is highly resistant to many antimicrobial agents and this resistance may result from the low drug permeability of outer membrane proteins, known as porins. | 2014 | 24788109 |
| analysis of the prevalence, secretion and function of a cell cycle-inhibiting factor in the melioidosis pathogen burkholderia pseudomallei. | enteropathogenic and enterohaemorrhagic escherichia coli express a cell cycle-inhibiting factor (cif), that is injected into host cells via a type iii secretion system (t3ss) leading to arrest of cell division, delayed apoptosis and cytoskeletal rearrangements. a homologue of cif has been identified in burkholderia pseudomallei (chbp; cif homologue in b. pseudomallei; bpss1385), which shares catalytic activity, but its prevalence, secretion and function are ill-defined. among 43 available b. pse ... | 2014 | 24809950 |