Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| nucleotide sequence analysis of the respiratory syncytial virus subgroup a cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate. | the complete nucleotide sequence of the rsv cpts-248/404 live attenuated vaccine candidate was determined from cloned cdna and was compared to that of the rsv a2/hek7 wild-type, cold-passaged cp-rsv, and cpts-248 virus, which constitute the series of progenitor viruses. rsv cpts-248/404 is more attenuated and more temperature sensitive (ts) (shut-off temperature 36 degrees) than its cpts-248 parent virus (shut-off temperature 38 degrees) and is currently being evaluated in phase i clinical trial ... | 1996 | 8918930 |
| vaccination with a low-oncogenic strain of rous sarcoma virus prevents visceral tumors in chickens. | the oncogenic potential of different strains of rous sarcoma virus (rsv) varies significantly in two mhc(b) congenic chicken lines cb and cc and in their f1 hybrids with the unrelated inbred line ia. the bryan high-titer pseudotype of rsv of antigenic subgroups d and b (bh-d, and bh-b) was highly oncogenic, eliciting mostly fatal, progressively growing tumors. visceral tumor formation and erythroblastosis were seen after challenge with bh-b in cb and cc chickens, but not in their f1 hybrids with ... | 1995 | 8919361 |
| efficient expression of pro-urokinase by human lymphoblastoid namalwa kjm-1 cells using moloney retroviral promoter. | we have compared the level of expression of several enhancer/promoters in human lymphoblastoid namalwa kjm-1 cells when fused to a common reporter gene. a cassette containing the pro-urokinase (pro-uk) coding sequence followed by the rabbit beta-globin and simian virus 40 (sv40) 3' nontranslated region was used for evaluation of the enhancer activity. cells containing moloney murine leukemia virus (mo-mulv) promoter had an average of 10-20 fold higher expression levels of pro-uk than those conta ... | 1995 | 8920107 |
| a colorimetric ldh assay for the titration of infectivity and the evaluation of anti-viral activity against ortho- and paramyxoviruses. | a rapid and precise screening assay was developed for in vitro evaluation of anti-orthomyxo- and anti-paramyxovirus agents. the procedure is spectrophotometrical assessment for viability of cells via extracellular leakage of lactic dehydrogenase (ldh). hmv-ii cells, a human melanoma cell line was found to be suitable for the titration of virus infectivity and screening of anti-viral agents for orthomyxo- and paramyxoviruses. comparative titration of infectivity of stock viruses by the ldh and th ... | 1995 | 8928191 |
| two tumor-associated membrane antigens defined by monoclonal antibodies in a transplantable sarcoma induced by rous sarcoma virus in rat. | two hybridoma clones have been produced by hybridization of murine myeloma cell line pai and splenocytes from balb/c mice immunized with cells from a transplantable sarcoma induced in rat by sr-rsv. the antibody produced by hybridoma clone 2c2 was of subclass igg3 and recognized a cell surface antigen of 52 kd. it only cross-reacted with cells from sr-rsv-induced sarcoma in hamster, but not with cells from the chicken rsv-induced sarcoma, nor with a number of methylcholanthrene sarcomas and vari ... | 1996 | 8931754 |
| preventing nosocomial infection in paediatric wards. | nosocomial infection is potentially a major problem in paediatric wards. however, there are methodological difficulties which hinder researchers in identifying the true extent of nosocomial infection. respiratory infections pose the greatest challenge, with the respiratory syncytial virus (rsv) being particularly problematic. however, rsv can be contained by strict implementation of isolation procedures. other major pathogens such as enteric bacteria and viruses can similarly be controlled throu ... | 1996 | 8932032 |
| laboratory diagnosis of acute lower respiratory tract viral infections in children. | this report summarizes and compares the results of complement fixation test (cft), virus isolation (vi), and direct immunofluorescence test (dif) for the antigen detection of respiratory syncytial virus (rsv), influenza a virus (flu a), and adenovirus in 62 children with acute lower respiratory tract infections (alri) in kuwait. it includes, as well, cft results for parainfluenza virus and mycoplasma pneumoniae. combining the three methods, a potential causative agent was identified in 56 (90 pe ... | 1996 | 8936958 |
| sequence of echinochloa hoja blanca tenuivirus rna-4. | the sequence is presented of rna-4 of echinochloa hoja blanca tenuivirus (ehbv), one of two tenuiviruses associated with rice cultivation in latin america (together with rice hoja blanca virus; rhbv). analysis of the sequence shows that the coding regions of ehbv rna-4 are closely related to those of rhbv rna-4. however, the intergenic region separating the two ambisense open reading frames, are highly distinct for the two viruses. the features of the rna and the comparisons with the sequences o ... | 1996 | 8938980 |
| sequence of echinochloa hoja blanca tenuivirus rna-3. | analysis of the sequence of the 2336 nucleotide rna-3 of echinochloa hoja blanca tenuivirus shows that it is closely related to rna-3 of rice hoja blanca tenuivirus, the principal virus disease of rice in latin america. this is especially true for the coding regions, where the viruses are almost 90% similar. however, the non-coding regions of rna-3 of these viruses, principally the intergenic region separating the two ambisense open reading frames, are only about 50% similar, suggesting that the ... | 1996 | 8938981 |
| surveillance of community-acquired viral infections due to respiratory viruses in rhone-alpes (france) during winter 1994 to 1995. | nasal swab from patients with acute flu-like illness were evaluated for the presence of respiratory viruses in the rhone-alpes region of france from 1 october 1994 through 2 may 1995. the relative frequencies and seasonal distributions of the specific viruses were assessed. in addition, virus type was correlated with specific clinical signs and symptoms. during the study, 962 samples were collected by 75 medical practitioners participating in the groupe regional d'observation de la grippe survei ... | 1996 | 8940439 |
| reverse transcription-polymerase chain reaction amplification of respiratory syncytial virus genome from neonatal nasal swab samples. | in order to make a rapid and accurate diagnosis of respiratory syncytial virus (rsv) infection, nasal swabs obtained from 14 neonates suspected of having this disease were examined for the presence of rsv genome by reverse transcription (rt) and nested polymerase chain reaction (pcr) amplification, along with enzyme immunoassay (eia), serum neutralization testing and virus isolation. the rt-pcr method was sensitive enough to detect a 0.1 50% tissue culture infectious dose (tcid50) per milliliter ... | 1996 | 8941998 |
| serological study of responses to selected pathogens causing respiratory tract infection in the institutionalized elderly. | in a prospective 2-year study, serological responses to selected pathogens were analyzed in 224 episodes of fever attributable to respiratory tract infection (51.8%) or of unknown source (48.2%) in 131 residents of two long-term-care facilities. a serological response was identified in 45 episodes (20.1%): chlamydia pneumoniae (14 episodes), haemophilus influenzae type b (1), influenza virus type a (14), respiratory syncytial virus (rsv;2), parainfluenza virus type 3 (7), c. pneumoniae and h. in ... | 1996 | 8953065 |
| developmental and environmental factors that enhance binding of bordetella pertussis to human epithelial cells in relation to sudden infant death syndrome (sids). | asymptomatic infection due to bordetella pertussis has been suggested to be one cause of sudden infant death syndrome (sids). we examined developmental and environmental factors previously found to affect binding of another toxigenic species, staphylococcus aureus, to human epithelial cells: expression of the lewis(a) antigen; infection with respiratory syncytial virus (rsv); exposure to cigarette smoke; and the inhibitory effect of breast milk on bacterial binding. binding of two strains of b. ... | 1996 | 8954353 |
| morphological studies of the respiratory syncytial virus induced bronchiolitis in experimentally infected calves. | rsv-infections of the lower respiratory tract in infancy and early childhood are the most frequent causes of a hyperreactive bronchial system and obstructive lung disease. studies concerning the morphological alterations of the bronchial mucosa during an rsv-infection are dependent on an experimental animal model. in this study the alterations of the lower respiratory tract from five infected colostrum-fed calves during the initial stage of the infection are described. brsv strain 375 was applie ... | 1996 | 8958555 |
| safety and immunogenicity of a respiratory syncytial virus subunit vaccine (pfp-2) in ambulatory adults over age 60. | the safety and immunogenicity of purified fusion protein (pfp-2) respiratory syncytial virus (rsv) vaccine was evaluated in a randomized placebo-controlled, double-blind study of 64 healthy adults over age 60. vaccination was well tolerated with no significant acute side-effects. twenty-nine of 33 vaccinees (87%) showed a greater than or equal to fourfold rise in serum igg to the f protein of rsv at 8 weeks post vaccination. twenty of 33 vaccine recipients (61%) had a greater than or equal to fo ... | 1996 | 8961507 |
| phase i study of a recombinant adenovirus-mediated gene transfer in lung cancer patients. | despite vigorous efforts at curbing tobacco consumption and aggressive combined-modality treatment programs, both the incidence of and the mortality from lung cancer have remained virtually unchanged in the last 10 years. more effective innovative therapies are clearly needed. the direct transfer into tumor cells of tumor suppressor genes or toxic gene products that specifically promote tumor cell death and spare nonmalignant cells is a potentially novel anticancer treatment approach that should ... | 1996 | 8961977 |
| [characteristics of anti-respiratory syncytial humoral immunity during persistence of respiratory syncytial virus antigens in adult patients with chronic obstructive bronchitis]. | specific humoral immune response has been studied in 8 patients with chronic obstructive bronchitis, in whom the persistence of rsv was detected, and in a group of patients without rsv persistence. humoral immunity in patients with rsv persistence vs. those with acute and chronic bronchitis without viral persistence was characterized by the following features: a) earlier and more active production of igg and, even more so, igm and iga; b) long persistence of high titers of anti-rsv igg, igm, and ... | 1996 | 8967073 |
| purification of autocrine growth factor from conditioned medium of rat sarcoma (xc) cells. | transformation of rat cells by rous sarcoma virus(es) induced the release of growth factors into serum-free conditioned media. an pr-rsv-transformed rat cell line, xc, produced and released polypeptide factors which promote anchorage-dependent and anchorage-independent growth of xc cells. one of the autocrine factors of xc cells was purified to homogeneity by four-step procedure: ultrafiltration, ion-exchange chromatography on monos, reverse-phase chromatography on spherisorb ods2 and gel filtra ... | 1996 | 8967958 |
| frequency of respiratory syncytial virus in hospitalized infants with lower acute respiratory tract infection in colombia. | in colombia, health workers obtained a nasopharyngeal wash from 103 infants aged less than 12 months hospitalized for acute lower respiratory infection (alri) at the general hospital of medellin during april 1994 to april 1995 so researchers could determine the frequency of alri caused by respiratory syncytial virus (rsv) in hospitalized children. immunofluorescence detected rsv infection in 43 (41.7%) patients. the presence of the following signs and symptoms allowed a clinical diagnosis of a ... | 1996 | 8970224 |
| transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the rela transcription factor as a mechanism producing airway mucosal inflammation. | the most common cause of epidemic pediatric respiratory disease, respiratory syncytial virus (rsv), stimulates interleukin-8 (il-8) synthesis upon infecting airway epithelium, an event necessary for the development of mucosal inflammation. we investigated the mechanism for enhanced il-8 production in human a549 type ii pulmonary epithelial cells. infection with sucrose-purified rsv (prsv) produced a time-dependent increase in the transcriptional initiation rate of the il-8 gene. transient transf ... | 1996 | 8971006 |
| inhibition of viral replication reverses respiratory syncytial virus-induced nf-kappab activation and interleukin-8 gene expression in a549 cells. | previous studies have demonstrated that respiratory syncytial virus (rsv) infection of airway epithelial cells results in the expression of a number of cytokines, such as interleukin-8 (il-8), that are transcriptionally regulated by nuclear factor kappab (nf-kappab). in the studies reported here, we demonstrate that treatment of rsv-infected a549 cells with 100 microg of ribavirin (a viral replication inhibitor) per ml results in reversal of rsv-induced nf-kappab activation, il-8 mrna expression ... | 1996 | 8971048 |
| respiratory syncytial virus-induced cytokine production by neonatal macrophages. | the induction of immunoregulatory cytokines il-1 beta, il-6, il-12, tumour necrosis factor-alpha (tnf-alpha) and interferon-gamma (ifn-gamma) was studied with neonatal (cord blood) monocyte-derived macrophages (mdm) after in vitro infection with respiratory syncytial virus (rsv). the expression of mrnas for these cytokines in rsv-infected mdm was examined by reverse transcriptase-polymerase chain reaction (rt-pcr). the activities of these cytokines were assayed by elisa. significant increase of ... | 1996 | 8973610 |
| update: respiratory syncytial virus activity--united states, 1996-97 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, results in an estimated 90,000 hospitalizations and 4500 deaths each year from lower respiratory tract disease in both infants and young children in the united states. outbreaks occur annually throughout the country. rsv activity in the united states is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes ... | 1996 | 8975116 |
| icam-1 expression and low-molecular-weight g-protein activation of human bronchial epithelial cells (a549) infected with rsv. | the airway epithelial cell of the lower respiratory tract is the primary host target cell for respiratory syncytial virus (rsv) infection. to estimate whether infected epithelial cells contribute to the inflammatory host response observed during the acute infection phase we analyzed the cell surface expression of intercellular adhesion molecule-1 (icam-1, cd54) on human bronchial epithelial cells (a549) following rsv infection and cytokine priming. the epithelial cells constitutively expressed i ... | 1996 | 8975880 |
| increase in cd23+ b cells in infants with bronchiolitis is accompanied by appearance of ige and igg4 antibodies specific for respiratory syncytial virus. | infection with respiratory syncytial virus (rsv) may induce asthma-like symptoms and rsv-specific ige in infected infants as a result of th2-like response to rsv. the effect of rsv infection on the expression of b cell antigens cd21 and cd23, putative participants in th2 responses, was investigated. samples from bronchiolitic infants (n = 19) were tested by three-color immunofluorescence flow cytometry during the acute phase of infection and 4-6 weeks later. in 6 of 10 rsv-positive infants, the ... | 1997 | 8985193 |
| virus-specific memory and effector t lymphocytes exhibit different cytokine responses to antigens during experimental murine respiratory syncytial virus infection. | mice sensitized to the g (attachment) or f (fusion) glycoproteins of respiratory syncytial virus (rsv) expressed different patterns of cytokine production and lung pathology when challenged by intranasal infection with rsv. five days after challenge, mice sensitized to g glycoprotein produced high levels of interleukin-4 (il-4) and il-5 in the lungs and spleens and developed extensive pulmonary eosinophilia, while mice sensitized to f glycoprotein produced il-2 and developed a mononuclear cell i ... | 1997 | 8985399 |
| identification of potential ctl epitopes of bovine rsv using allele-specific peptide motifs from bovine mhc class i molecules. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young infants and housed calves. depletion of cd8+ lymphocytes from calves inhibited their ability to clear the virus from the nasopharynx and lungs. to study these cells further, a cytotoxic t lymphocyte (ctl) assay was established. ctl could be demonstrated in the peripheral blood of gnotobiotic calves 7-10 days post infection (p.i.) with rsv and in lungs 10 days p.i. this response was both mhc-restricte ... | 1996 | 8988867 |
| reduction of respiratory syncytial virus hospitalization among premature infants and infants with bronchopulmonary dysplasia using respiratory syncytial virus immune globulin prophylaxis. the prevent study group. | to determine the safety and efficacy of monthly prophylaxis with respiratory syncytial virus immune globulin, intravenous (rsv-igiv) for reduction of the incidence of rsv-associated hospitalization. | 1997 | 8989345 |
| stabilization of respiratory syncytial virus (rsv) against thermal inactivation and freeze-thaw cycles for development and control of rsv vaccines and immune globulin. | a high-titered and stable respiratory syncytial virus (rsv) is essential for the development of rsv vaccines and quality control of vaccines and rsv immune globulin. we increased the virus titer of rsv seed stock, and virus preparations made from this seed stock, 100 times by removing defective interfering particles using limiting dilution procedure. rsv preparations made from the new seed stock had infectivity titers ranging from 10(7.6) to 10(8.2) tcid50 per ml for five lots made over a period ... | 1996 | 8994316 |
| molecular characterization of the complex sex-chromosome heterochromatin in the rodent microtus chrotorrhinus. | the sex chromosomes of microtus chrotorrhinus are unusually large compared to those of other mammals, comprising about 20% of the karyotype and containing substantial amounts of constitutive heterochromatin. previous studies have described two highly repeated dna families (msat-160 and msat-2570) that localize to this heterochromatin (modi, 1992, 1993c). the present report describes a third satellite dna family (termed msat-21) in m. chrotorrhinus that is also located in the sex heterochromatin. ... | 1996 | 8995489 |
| inducible retroviral vectors regulated by lac repressor in mammalian cells. | we have developed lac repressor-regulated retroviral expression vectors that are induced by beta-galactosides upon stable transduction in mammalian cells. these vectors, derived from a moloney-virus-based vector lncx, contain an internal rous sarcoma virus (rsv) or cytomegalovirus (cmv) promoter, coupled with 2 to 4 lac operator sequences and placed in anti orientation relative to the retroviral long terminal repeat (ltr). three different vectors were tested in stably infected mass populations o ... | 1996 | 8996098 |
| mechanism of rsv-induced il-8 gene expression in a549 cells before viral replication. | previous studies demonstrated that respiratory syncytial virus (rsv) infection of a549 cells induced interleukin (il)-8 gene expression and protein release from the cells as early as 2 h after treatment [m. a. fiedler, k. wernke-dollries, and j. m. stark. am. j. physiol. 269 (lung cell. mol. physiol. 13): l865-l872, 1995; j. g. mastronarde, m. m. monick, and g. w. hunninghake. am. j. respir. cell mol. biol. 13: 237-244, 1995]. furthermore, the effects of rsv at the 2-h time point were not depend ... | 1996 | 8997267 |
| respiratory syncytial virus immune globulin intravenous. | to review the clinical data detailing the use of respiratory syncytial virus immune globulin intravenous (rsv-igiv) in infants and children. data sources; a medline search (1990-1996) was used to identify all publications that dealt with rsv-igiv clinical trials, pharmacology, and pharmacokinetics in infants and children. bibliographies of articles were also used. | 1997 | 8997472 |
| isolation of viruses from clinical specimens in microtitre plates with cells inoculated in suspension. | virus isolation is essential for the provision of a full diagnostic virology service. present methods are time consuming, expensive and relatively inflexible for routine use. our objective was to audit our existing virus isolation system and to develop a sensitive, flexible virus isolation system which could be adapted for use in a busy routine laboratory which is required to provide a service for a wide range of clinical situations. we carried out a pilot study which compared conventional rolle ... | 1996 | 9002075 |
| rhinovirus infection associated with serious lower respiratory illness in patients with bronchopulmonary dysplasia. | to determine the characteristics of rhinovirus infection in patients with bronchopulmonary dysplasia. | 1997 | 9002100 |
| white blood cell count, c-reactive protein and erythrocyte sedimentation rate in respiratory syncytial virus infection of the lower respiratory tract. | laboratory findings such as white blood cell (wbc) count, c-reactive protein (crp) concentration and erythrocyte sedimentation rate (esr) level in patients with bronchiolitis, bronchopneumonia and lobar pneumonia caused by respiratory syncytial virus (rsv) were studied. the diagnosis of having rsv infection of the lower respiratory tract was made on the presence of rsv antigen in nasopharyngeal specimens by means of enzyme immunoassay, on chest x-ray appearances and clinical manifestations. the ... | 1996 | 9002292 |
| detection of antibodies to respiratory syncytial virus attachment and nucleocapsid proteins with recombinant baculovirus-expressed antigens. | the ability to measure antibodies against individual respiratory syncytial virus (rsv) proteins is important in the analysis of immune responses to rsv. we expressed the nucleocapsid (n) protein and the group a and b rsv attachment (g) proteins from recombinant baculoviruses. the three recombinant rsv proteins were used individually in an enzyme-linked immunosorbent assay (elisa; bac-elisa for results from assays of all three proteins). the bac-elisa results were compared to the results obtained ... | 1997 | 9003595 |
| prevalence of antibodies against hep-2 cell antigen in infants and children hospitalized with respiratory syncytial virus infection. | infants with respiratory syncytial virus (rsv) infection were shown to have antibodies against hep-2 cell antigen present in rsv-antigen preparation used for immunoblot analysis. the prevalence of anti hep-2 cell antibodies was examined in infants hospitalized for rsv infection (n = 49, median age 121 days) compared to rotavirus infected children (n = 30, median age 114 days) and to healthy controls (n = 20, median age 150 days). the immunoblot analysis with rsv-infected and non-infected hep-2 c ... | 1996 | 9007586 |
| physiological effects of adenoviral gene transfer of sarcoplasmic reticulum calcium atpase in isolated rat myocytes. | in myocardial cells, relaxation is governed primarily by the sarcoplasmic reticulum (sr) ca(2+)-atpase transporting enzyme, which regulates ca2+ sequestration into the sr. human and experimental cardiomyopathies are associated with reduced sr ca(2+)-atpase activity. | 1997 | 9008460 |
| comparison of the antiviral efficacy of ribozymes and antisense rna directed against bovine leukemia virus rex/tax. | despite the theoretical attraction of ribozymes, which cleave their target rna, as compared with antisense rna, which only blocks translation, few studies have assessed the relative efficacy of ribozymes and antisense rna directed against the identical target sequence. previously, we described the efficacy of a hammerhead ribozyme targeted against rex/tax, a regulatory gene of bovine leukemia virus (blv). in this study, we asked whether antisense rna targeted against the same site would also be ... | 1996 | 9012866 |
| maturation of respiratory syncytial virus within hep-2 cell cytoplasm. | electron microscopy of hep-2 cells infected with respiratory syncytial virus (rsv) strain long revealed the maturation of rsv on an ultrastructural level. the results showed that the virus maturated by two different pathways. in one of them, the virus assembled and matured before reaching the plasma membrane on the internal vesicle membrane within cytoplasm. the mature virus was delivered to the plasma membrane and to the extracellular space most likely by the transport vesicles and exocytosis. ... | 1996 | 9014011 |
| treatment of respiratory failure due to respiratory syncytial virus pneumonia with natural surfactant. | we describe two infants suffering from severe pneumonia caused by respiratory syncytial virus (rsv) infection and needing mechanical ventilation with both high ventilator settings and a high fraction of inspired oxygen. the severity of the respiratory failure and the possibility of decreased and/or altered surfactant production led us to treat these infants with intratracheal instillation of natural surfactant. this resulted in an improvement of lung compliance and a decrease in the amount of ox ... | 1996 | 9016476 |
| splicing efficiency of human immunodeficiency virus type 1 tat rna is determined by both a suboptimal 3' splice site and a 10 nucleotide exon splicing silencer element located within tat exon 2. | we have previously demonstrated that an exon splicing silencer (ess) is present within human immunodeficiency virus type 1 (hiv-1)tat exon 2. this 20 nucleotide (nt) rna element acts selectively to inhibit splicing at the upstream 3'splice site (3'ss #3) flanking this exon. in this report, we have used in vitro splicing of mutated rna substrates to determine the sequences necessary and sufficient for the activity of the ess. the activity of the ess within tat exon 2 maps to a 10 nt core sequence ... | 1997 | 9016638 |
| antigenic and molecular analyses of the variability of bovine respiratory syncytial virus g glycoprotein. | antigenic variation among eight bovine respiratory syncytial virus (brsv) isolates was determined using monoclonal antibodies (mabs) specific for the attachment (g) protein. two major (and one intermediate) subgroups were identified, as well as one strain that did not fit any pattern. the subgroups could also be differentiated on the basis of the mr of the f protein cleavage product, f2. the nucleotide sequence of the g gene of seven of the brsv strains was determined and compared with published ... | 1997 | 9018058 |
| androgen-dependent cell cycle arrest and apoptotic death in pc-3 prostatic cell cultures expressing a full-length human androgen receptor. | to assess the function of androgen receptor in androgen-independent prostate cancer cells, human pc-3 prostate carcinoma cells, which lack androgen receptor (ar) expression, were transfected with a full length human ar cdna sequence inserted into an episomal expression vector system. several clonal lines of transfected cells expressing varying levels of a 110 kda ar, as determined by immunoblotting and ligand binding assay, were isolated. the expressed ectopic receptors displayed nuclear binding ... | 1997 | 9027364 |
| analysis of recombinant adeno-associated virus packaging and requirements for rep and cap gene products. | adeno-associated virus (aav) is a human parvovirus currently being developed as a vector for gene therapy applications. because the gene transfer vector commonly retains only the aav terminal repeats, propagation of recombinant aav (raav) requires that the viral replication (rep) and capsid (cap) proteins be supplied in trans. in an effort to optimize the production of these vectors, a panel of helper plasmids was constructed to determine if expression of the rep and/or cap genes is a limiting f ... | 1997 | 9032320 |
| altered rous sarcoma virus gag polyprotein processing and its effects on particle formation. | proteolytic processing of the rous sarcoma virus (rsv) gag precursor was altered in vivo through the introduction of amino acid substitutions into either the polyprotein cleavage junctions or the pr coding sequence. single amino acid substitutions (v(p2)s and p(p4)g), which are predicted from in vitro peptide substrate cleavage data to decrease the rate of release of pr from the gag polyprotein, were placed in the nc portion of the nc-pr junction. these substitutions do not affect the efficiency ... | 1997 | 9032340 |
| persistence of respiratory syncytial virus (rsv) infection and development of rsv-specific igg1 response in a guinea-pig model of acute bronchiolitis. | acute respiratory syncytial virus (rsv) bronchiolitis in children can result in sequelae of recurrent wheezing and asthma and production of rsv-specific immunoglobulin e (ige), but the pathogenesis of these sequeleae is poorly understood. guinea-pigs experimentally inoculated with human rsv show histological evidence of acute bronchiolitis and chronic persistence of viral antigens and genome in the lungs; whether this persistence is due to infectious replicating virus, and whether infected anima ... | 1997 | 9032486 |
| acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (l) gene. | a cold-passaged (cp) temperature-sensitive (ts) mutant of human respiratory syncytial virus designated rsv cpts-248 was previously derived by random chemical mutagenesis of the non-ts mutant cp-rsv that possesses one or more host range mutations. we previously demonstrated in rodents and seronegative chimpanzees that the cpts-248 virus is more attenuated than cp-rsv and is more stable genetically than previously isolated rsv ts mutants. in the present study, we determined that the acquisition of ... | 1996 | 9035372 |
| guidelines for prevention of nosocomial pneumonia. centers for disease control and prevention. | this document updates and replaces cdc's previously published "guideline for prevention of nosocomial pneumonia" (infect control 1982;3:327-33, respir care 1983;28:221-32, and am j infect control 1983;11:230-44). this revised guideline is designed to reduce the incidence of nosocomial pneumonia and is intended for use by personnel who are responsible for surveillance and control of infections in acute-care hospitals; the information may not be applicable in long-term-care facilities because of t ... | 1997 | 9036304 |
| adenovirus-mediated suicide gene therapy for experimental bladder cancer. | to determine the feasibility, safety, and efficacy of suicide gene therapy using adenoviral-mediated herpes simplex virus thymidine kinase gene (hsv-tk) and the prodrug ganciclovir (gcv) in a murine model of human transitional cell carcinoma. | 1997 | 9037277 |
| inhibition of respiratory syncytial virus replication by antisense oligodeoxyribonucleotides. | oligodeoxyribonucleotides targeted against respiratory syncytial virus (rsv) genomic rna inhibited rsv replication in cell culture by an apparent antisense mechanism. hep-2 cells were infected with rsv strain a2 and incubated in the presence of oligonucleotides. virus replication was measured by enzyme-linked immunosorbent assay (elisa), virus yield assay, or production of specific rsv mrnas. using elisa, 50% effective concentration (ec50) values were about 0.5-1 microm for an antisense oligonuc ... | 1997 | 9037376 |
| hypotension in transgenic mice overexpressing human bradykinin b2 receptor. | bradykinin binds to its receptor at target organs and exerts a wide spectrum of biological activities including vasodilation, smooth muscle contraction and relaxation, pain, and inflammation. to gain a better insight into the physiological function of this potent vasoactive peptide, we created transgenic mice that harbor the human bradykinin b2 receptor transgene under the control of the rous sarcoma virus 3'-ltr promoter (rsv-chbkr). expression of hbkr in these transgenic mice was identified in ... | 1997 | 9039147 |
| respiratory syncytial virus (rsv) immune globulin intravenous therapy for rsv lower respiratory tract infection in infants and young children at high risk for severe rsv infections: respiratory syncytial virus immune globulin study group. | to evaluate the efficacy of high-titer intravenous respiratory syncytial virus immune globulin (rsvig) in the treatment of children at high risk for severe rsv infection who were hospitalized with proven rsv. | 1997 | 9041304 |
| respiratory syncytial virus induces selective production of the chemokine rantes by upper airway epithelial cells. | the presence of histamine and eosinophil cationic protein in nasopharyngeal secretions of infants with respiratory syncytial virus (rsv)-induced bronchiolitis implies the activation of basophil and eosinophil leukocytes, but the specific mechanism of their recruitment has not been elucidated. chemokines are potent and selective leukocyte chemotactic molecules that are also expressed by airway epithelial cells. therefore, the pattern of chemokines produced in response to rsv infection was investi ... | 1997 | 9041319 |
| extracorporeal life support for the treatment of viral pneumonia: collective experience from the elso registry. extracorporeal life support organization. | viral pneumonia is the most common indication for pediatric extracorporeal life support (ecls). despite this fact, no previous studies have directly stratified patient outcome according to viral etiology. | 1997 | 9044128 |
| adenovirus-mediated delivery of human kallistatin gene reduces blood pressure of spontaneously hypertensive rats. | human kallistatin, or human tissue kallikrein-binding protein (hkbp), is a serine proteinase inhibitor (serpin). transgenic mice overexpressing rat kallikrein-binding protein are hypotensive. to elucidate therapeutic potentials of kallistatin in hypertension, the human kallistatin gene in an adenoviral vector was directly introduced into spontaneously hypertensive rats (shr) through portal vein injection. the kallistatin cdna construct (rsv-chkbp) under the promoter control of rous sarcoma virus ... | 1997 | 9048201 |
| targeting rna decay with 2',5' oligoadenylate-antisense in respiratory syncytial virus-infected cells. | treatment of human cells with 2',5' oligoadenylate covalently linked to antisense (2-5a-antisense) results in the selective cleavage of targeted rna species by 2-5a-dependent rnase l. here we show that 2-5a-antisense containing stabilizing modifications at both termini are effective in suppressing the replication of respiratory syncytial virus (rsv) in human tracheal epithelial cells. the affinity of 2-5a-antisense for different regions in the rsv m2 and l mrnas was predicted from a computer-gen ... | 1997 | 9050883 |
| cell lines containing and expressing the human herpesvirus 6a ts gene are protected from both h-ras and bpv-1 transformation. | human herpesvirus 6a (hhv-6a) strain u1102 was previously shown to contain a 1473 bp transformation suppressor gene (ts) (araujo et al., 1995). ts inhibited transformation of nih3t3 cells by h-ras and transcription of the h-ras and human immunodeficiency type 1 (hiv-1) promoters in transient transfection experiments. in the current study, stable nih3t3 cell lines expressing ts protein were established by transfection with prc-ts containing the ts gene under the control of the rous sarcoma virus ... | 1997 | 9050993 |
| respiratory syncytial virus infection among hospitalized young children with acute lower respiratory illnesses in al ain, uae. | to establish the frequency and clinical pattern of respiratory syncytial virus (rsv) infection in the region, children under 3 years of age admitted for acute lower respiratory illness during two winter seasons of the years 1993-94 and 1994-95 were studied prospectively. seventy two cases were diagnosed to have rsv infection among the 252 studied, representing 28.57% of these patients. the overall infection rate was 32.1% and 36.5% respectively for the two studied winter seasons. among these chi ... | 1996 | 9057448 |
| chicken yb-2, a y-box protein, is a potent activator of rous sarcoma virus long terminal repeat-driven transcription in avian fibroblasts. | we have previously reported on the cloning and characterization of chk-yb-2, a novel member of the y-box family of proteins, that binds to the sequence 5'-gtaccacc-3' present on the noncoding strand of the rous sarcoma virus (rsv) long terminal repeat (ltr) in a single-strand-specific manner. here, we demonstrate that deletion or mutation of this motif not only eliminates chk-yb-2 binding in vitro but also down-regulates rsv ltr-driven transcription in avian cells. selective abrogation of chk-yb ... | 1997 | 9060644 |
| improved oxygenation after discontinuing neuromuscular blockade. | to evaluate the effects of prolonged neuromuscular blockade (nmb) on oxygenation and duration of mechanical ventilation in children with respiratory failure. | 1997 | 9069009 |
| respiratory syncytial virus infection in the elderly. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections in infants and children throughout the world. respiratory syncytial virus infections in the elderly represent reinfections in the hosts who have had many prior episodes. thus, rsv infections are usually not considered serious in adults, since reinfections are generally known to result in mild disease. nevertheless, in adults, as in children, the infection has been reported to cause altered airway res ... | 1996 | 9072495 |
| persistence of airway hyperresponsiveness and viral antigen following respiratory syncytial virus bronchiolitis in young guinea-pigs. | respiratory syncytial virus (rsv) bronchiolitis in infancy is known to be followed by chronic respiratory symptoms and airway hyperresponsiveness in a subgroup of patients. to further investigate the pathogenesis of rsv-induced chronic airway pathology, we infected young guinea-pigs at 4 weeks of age with rsv applied as an aerosol (n=30), and control guinea-pigs with virus-free culture medium (n=24). infection was confirmed by positive antibody titre to rsv after 6 weeks, and by typical patholog ... | 1997 | 9072998 |
| respiratory syncytial virus triggers synthesis of il-6 in balb/c mouse alveolar macrophages in the absence of virus replication. | cytokines produced by alveolar macrophages are likely involved in the regulation of the immune response arising from respiratory syncytial virus (rsv) infection. both infectious and uv-inactivated rsv were effective in inducing balb/c mouse alveolar macrophages to synthesize increased levels of il-6 mrna and secreted il-6 protein. no increase in il-1beta (either mrna or secreted protein) was observed. the augmented production of il-6 was activated by purified virus and was reduced by pretreating ... | 1997 | 9073384 |
| follow-up of children with respiratory syncytial virus bronchiolitis in 1986 and 1987: potential effect of ribavirin on long term pulmonary function. the bronchiolitis study group. | hospitalization for respiratory syncytial virus (rsv) bronchiolitis is considered a risk factor for reactive airway disease and/or abnormal pulmonary function tests (pfts) later in childhood. this study attempted to assess this observation with newer pfts and to determine whether ribavirin therapy of initial rsv infection may affect the long term outcome. | 1997 | 9076814 |
| dynamic distribution of an antigen involved in differentiation of quail myoblasts transformed with rous sarcoma virus: i. requirement of its quantitative expression on the cell surface for myoblast fusion. | a monoclonal antibody, h-145, the antigen of which is a glycoprotein with a molecular weight of 116 kda, inhibits the fusion of quail skeletal myoblasts transformed with the temperature-sensitive mutant of rous sarcoma virus (ts-rsv) (qm-rsv cells). its antigen shows a unique distribution pattern during myogenic differentiation, and is required continuously for the inhibition as reported previously. the h-145 antigen is expressed from as early as the presumptive myoblast stage, and its expressio ... | 1996 | 9078409 |
| severity of respiratory syncytial virus infection is related to virus strain. | the relationship between respiratory syncytial virus (rsv) strain and disease severity was assessed in 265 hospitalized infants over a 3-year period (1988-1991). a severity index of clinical and physiologic parameters was used to grade illness severity. multivariate analysis of 134 infants infected with group a rsv strains and 131 infants infected with group b strains indicated that prematurity, underlying medical conditions, group a rsv infection, and age < or =3 months were independently assoc ... | 1997 | 9086135 |
| immunoprophylaxis of group b respiratory syncytial virus infection in cotton rats. | two antigenic groups of respiratory syncytial virus (rsv) have been identified: a (rsv/a) and b (rsv/b). topical administration of human igg screened for high titers of antibody to rsv/a (rsvig) is protective against rsv/a infection in the cotton rat model. the study attempted to determine if topical rsvig would also be protective against rsv/b. cotton rats were pretreated intranasally with rsvig or with monospecific antiserum obtained from animals previously infected with rsv/a or rsv/b (day 0) ... | 1997 | 9086152 |
| the guinea pig as a model for the study of maternal immunization against respiratory syncytial virus infections in infancy. | maternal immunization might protect infants from severe disease due to respiratory syncytial virus (rsv). guinea pigs are susceptible to infections with rsv and transfer antibodies to their offspring prenatally. pregnant guinea pigs were immunized by infection with rsv and their offspring were challenged intranasally with rsv. pulmonary viral replication was compared among the pups born to immunized mothers (group a) and the pups from nonimmune mothers (group b) in two studies. mean (+/-sd) log1 ... | 1997 | 9086153 |
| respigam: an rsv immune globulin. | respiratory syncytial virus immune globulin has been shown to prevent or attenuate rsv lower respiratory tract infection in infants under 24 months of age with bpd or who were born prematurely (< or = 35 weeks gestation). the use of rsv-ig is expensive. the estimated average cost of prophylaxis for a season is $4,000 to $5,000 per infant. because of the cost of rsv-ig, and because viral transmission is possible when any blood product is administered, careful consideration of which children shoul ... | 1996 | 9087091 |
| standardization of enzyme-linked immunosorbent assays (elisas) for quantitative estimation of antibodies specific for infectious bovine rhinotracheitis virus, respiratory syncytial virus, parainfluenza-3 virus, and bovine viral diarrhea virus. | commercial enzyme-linked immunosorbent assays (elisas) for detection of serum antibodies to bovine viral diarrhea virus (bvdv), parainfluenza-3 virus (pi3v), respiratory syncytial virus (rsv), and infectious bovine rhinotracheitis virus (ibrv) were standardized to give a quantitative result when testing was performed at a single optimum dilution. for each test, serum samples were titrated and their end point titers calculated by an algebraic method directly from a plot of each titration series a ... | 1997 | 9087921 |
| respiratory syncytial virus bronchiolitis. | respiratory syncytial virus (rsv) bronchiolitis is associated with the clinical signs and symptoms of small airway obstruction. a major public health problem throughout the world, this condition is responsible for significant morbidity and mortality. management is primarily preventive, through strict hand washing, avoidance of exposure during the respiratory illness season and intravenously administered prophylactic anti-rsv immune globulin, especially in selected small infants with underlying c ... | 1997 | 9092277 |
| respiratory syncytial virus immune globulin intravenous: indications for use. american academy of pediatrics committee on infectious diseases, committee on fetus and newborn. | respiratory syncytial virus immune globulin intravenous (rsv-igiv) has been approved by the food and drug administration for use in the prevention of severe rsv infections in infants and children younger than 24 months with bronchopulmonary dysplasia or a history of premature birth (< or = 35 weeks of gestation). rsv-igiv administered monthly during the rsv season resulted in a 41% to 65% reduction in hospitalization rates in two clinical trials; however, rsv-igiv is costly, and intravenous admi ... | 1997 | 9093323 |
| analysis of linear epitopes recognised by the primary human antibody response to a variable region of the attachment (g) protein of respiratory syncytial virus. | the sites of linear epitopes in a variable region of the attachment (g) glycoprotein of respiratory syncytial virus (rsv) that are recognised by the human antibody response were examined. two sets of overlapping 12mer peptides each representing the carboxy-terminal 84 or 85 amino acids of the g protein of two group a isolates of human rsv were synthesised. these peptides were analysed using enzyme-linked immunosorbant assays (elisa) for their reactions with sera obtained from infants with primar ... | 1997 | 9093944 |
| antigenic structure of the central conserved region of protein g of bovine respiratory syncytial virus. | epitopes were resolved at the amino acid level for nine monoclonal antibodies (mabs) directed against the central conserved region of protein g of bovine respiratory syncytial virus (brsv-g). peptide binding studies showed which amino acids in the epitope contributed to antibody binding. the details of the epitopes were compared with the high-resolution structure of a synthetic peptide corresponding to the central conserved region of brsv-g, and this indicated which face of the central conserved ... | 1997 | 9094683 |
| acute respiratory distress syndrome caused by respiratory syncytial virus. | acute respiratory distress syndrome (ards) complicating severe respiratory syncytial virus (rsv) infection has been described in only a few infants. in contrast to the low mortality rates usually associated with rsv infections (< 5%), mortality rates in the range of 40-70% have been reported in pediatric patients with ards. however, studies on patients with ards are usually lumped with respect to causation, and the disease course of rsv-induced ards has not been previously studied. we examined t ... | 1997 | 9094725 |
| twenty years of outpatient respiratory syncytial virus infection: a framework for vaccine efficacy trials. | respiratory syncytial virus (rsv) is the most important viral respiratory pathogen of infancy and childhood. much has been written about inpatients with severe disease. inpatients, however, represent only a minority of rsv-infected children. we studied the characteristics of symptomatic outpatient rsv infection in healthy children to gain a better understanding of rsv disease and to provide a background for the testing of intervention strategies in children without high-risk conditions. | 1997 | 9099764 |
| does ribavirin impact on the hospital course of children with respiratory syncytial virus (rsv) infection? an analysis using the pediatric investigators collaborative network on infections in canada (picnic) rsv database. | to determine the relationship between receipt of aerosolized ribavirin and the hospital course of high-risk infants and children with respiratory syncytial virus (rsv) lower respiratory infection (lri). | 1997 | 9099772 |
| picnic (pediatric investigators collaborative network on infections in canada) study of the role of age and respiratory syncytial virus neutralizing antibody on respiratory syncytial virus illness in patients with underlying heart or lung disease. | to determine the effects of age and respiratory syncytial virus (rsv) antibody status on frequency and severity of rsv infections in children with underlying heart or lung disease. | 1997 | 9099774 |
| inducible high-level expression vector for mammalian cells, pef-lac carrying human elongation factor 1alpha promoter and lac operator. | we have constructed an inducible high-level expression vector, pef-lac. pef-lac has a modified human polypeptide chain elongation factor 1alpha (ef-1alpha) promoter containing three lactose operator sequences. using the cat reporter gene, we characterized the transcriptional activity of pef-lac. in the transient transfection of nih3t3 and baf3 cells, the transcriptional activity of pef-lac was higher than that of the original human elongation factor 1alpha promoter, simian virus 40 (sv40) promot ... | 1997 | 9099894 |
| [expression of the cmv-lacz- and rsv-lacz-genes in transgenic fish and mouse embryos]. | the bacterial gene for beta-galactosidase under the control of ltr from either human cytomegalovirus (cmv-lacz) or the rous sarcoma virus (rsv-lacz) was injected into fertilized eggs of misgurnus fossilis l. loaches and f1 hybrid mice (cba x c57bl). expression of the cmv-lacz was observed in almost 100% of the loach embryos and larvae for two months following the first day of embryonic development. in some cases, expression points were located only on either the right or the left side of a fish. ... | 1996 | 9102356 |
| potent inhibition of respiratory syncytial virus by polyoxometalates of several structural classes. | a series of polyoxometalates (pom) were synthesized and evaluated for anti-respiratory syncytial virus (rsv) activity. pom containing zirconium, tungsten, silicon, platinum, niobium or germanium of a variety of structural types have been evaluated. sixteen of the compounds had very striking anti-rsv activity against a clinical isolate, utah 89, with median effective concentration (ec50) values < or = 3 microm and selective indices > 80 as determined by viral cytopathic inhibition effect, neutral ... | 1997 | 9107383 |
| rsv infection of human airway epithelial cells causes production of the beta-chemokine rantes. | infection of airway epithelial cells with respiratory syncytial virus (rsv) results in the production of a restricted number of cytokines, which may modulate the inflammatory response to infection. to get a better understanding of epithelial cell-mediated inflammatory processes in rsv disease, the aim of the present study was to identify the production of mononuclear cell/eosinophil/mast cell inflammatory chemokines [monocyte chemotactic protein (mcp)-1, mcp-3, macrophage inflammatory protein-1b ... | 1997 | 9124609 |
| expression of mxa protein in blood lymphocytes discriminates between viral and bacterial infections in febrile children. | interferons (ifns), which are induced by viruses, form an essential part of host's defense systems against viral infections. the antiviral actions of ifns are mediated by several ifn-inducible gene products, one of which is mx protein. to evaluate whether mxa protein expression in lymphocytes could function as an indicator of endogenous ifn production in children with acute febrile illness, we analyzed mxa protein levels in peripheral blood lymphocytes by flow cytometry in the acute phase of the ... | 1997 | 9128286 |
| enhanced cytokine production by milk macrophages following infection with respiratory syncytial virus. | paired samples of milk and serum collected 3 days postpartum from 20 women were tested for the presence and level of interleukin (il)-1, il-6, il-12, tumor necrosis factor alpha (tnf-alpha), and interferon-gamma (ifn-gamma) by enzyme immunoassay. the expression of these cytokine mrnas in milk macrophages from eight donors were semiquantitatively analyzed by reverse transcriptase-polymerase chain reaction. the effects of respiratory syncytial virus (rsv) infection on cytokine production were dete ... | 1997 | 9129213 |
| respiratory syncytial virus specific serum antibodies in infants under six months of age: limited serological response upon infection. | the decline of maternal respiratory syncytial virus (rsv) specific serum antibodies was studied in 45 children during the first 6 months of life, using a virus neutralization assay and competition elisas measuring fusion protein and glycoprotein specific antibodies. in all children rsv neutralizing antibodies were demonstrated at birth, with titers ranging from 33 to 1382. the calculated mean half life of these antibodies was 26 days. furthermore, in a group of 38 children with suspected rsv inf ... | 1997 | 9131465 |
| growth factor ligands of tyrosine kinase receptors activate the rous sarcoma virus promoter by a ras- and raf-dependent mechanism. | in pc12 cells, ngf and other ligands of tyrosine kinase receptors increased the activity of the rous sarcoma virus (rsv) promoter. igf-1 stimulated the rsv promoter in sh-sy5y neuroblastoma cells. this promoter was also activated by oncogenic ras and raf in different cell types, and growth factor induction was inhibited by expression of dominant negative forms of ras and raf, showing that its effect is mediated by a ras- and raf-dependent mechanism. therefore, the rsv promoter should not be used ... | 1997 | 9133605 |
| immunological responses to respiratory syncytial virus infection in infancy. | to determine whether there is evidence of immunological responses in infants with respiratory syncytial virus (rsv) bronchiolitis by measuring inflammatory mediators in peripheral blood and, if found, whether these related to the severity of illness. | 1997 | 9135260 |
| detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture. | rt-pcr was compared with tissue culture to detect rsv from nasopharyngeal aspirates. rt-pcr was more sensitive and specific than tissue culture method. rt-pcr has sensitivity and specificity of 100% and 97%, respectively. the results indicate that rt-pcr can be used for detection of rsv in clinical specimens. | 1995 | 9139376 |
| phosphoprotein profile analysis of ruminant respiratory syncytial virus isolates. | to determine the apparent molecular weight for 24 ruminant respiratory syncytial viruses (rsv) on the basis of differences in the electrophoretic mobility of the phosphoprotein (p protein). | 1997 | 9140554 |
| subgroup specific protection of mice from respiratory syncytial virus infection with peptides encompassing the amino acid region 174-187 from the g glycoprotein: the role of cysteinyl residues in protection. | we identified subgroup specific protective epitopes represented by the amino acid regions 174-187 and 171-187 of the g glycoproteins from respiratory syncytial virus (rsv), subgroups a and b. mice immunized with coupled synthetic peptides corresponding to either the region 174-187 containing a cys186-->ser substitution or to the native region 171-187 were completely resistant to rsv infection but only to the respective virus. the protective activities of the peptides 174-187 were dependent on th ... | 1997 | 9141214 |
| induction of protective immunity in rodents by vaccination with a prokaryotically expressed recombinant fusion protein containing a respiratory syncytial virus g protein fragment. | a subunit approach to the development of a respiratory syncytial virus (rsv) vaccine was investigated. it involved the production, in escherichia coli, of an rsv (long) g protein fragment (g2na) as a c-terminal fusion partner to an albumin binding region (bb) of streptococcal protein g. g2na incorporated amino acid residues 130-230 and was specifically recognized by murine anti-rsv-a polyclonal serum. in mice, intraperitoneal immunization with bbg2na induced high anti-rsv-a serum elisa titers an ... | 1997 | 9143271 |
| cationic liposome-mediated expression of hiv-regulated luciferase and diphtheria toxin a genes in hela cells infected with or expressing hiv. | hiv-regulated expression of the diphtheria toxin a fragment gene (hiv-dt-a) is a potential gene therapy approach to aids. since cationic liposomes are safe and non-immunogenic for in vivo gene delivery, we examined whether lipofectamine or dmrie reagent could mediate the transfection of hiv-dt-a (ptha43) or the hiv-regulated luciferase gene (pluca43) into hiv-infected or uninfected hela cells. pluca43 was expressed at a 10(3)-fold higher level in hela/lav cells than in uninfected hela cells, whi ... | 1997 | 9150276 |
| serum eosinophil cationic protein and interleukin-5 in children with bronchial asthma and acute bronchiolitis. | the aim of our study was to evaluate the clinical applicability of serum eosinophil cationic protein (ecp), interleukin-5 (il-5) and total eosinophil counts in childhood asthma and bronchiolitis. these parameters were measured in 44 children aged 12-84 months with moderate and mild asthma during symptomatic and asymptomatic phases of disease. fifteen of the patients were included at the time of admission to hospital due to an acute asthmatic attack, and ten of these were also examined one month ... | 1996 | 9151339 |
| t cell source of type 1 cytokines determines illness patterns in respiratory syncytial virus-infected mice. | manipulation of the cytokine microenvironment at the time of vaccination can influence immune responses to remote challenge, providing a strategy to study the molecular pathogenesis of respiratory syncytial virus (rsv) vaccine-enhanced disease in the mouse model. although treatment with antibody against il-4 or recombinant il-12 (ril-12) at the time of formalin-inactivated rsv vaccination induced a similar shift in the pattern of cytokine mrna expression upon live virus challenge, anti-il-4 trea ... | 1997 | 9151790 |
| in vitro assembly of virus-like particles with rous sarcoma virus gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles. | retroviruses are unusual in that expression of a single protein, gag, leads to budding of virus-like particles into the extracellular space. we have developed conditions under which virus-like particles are formed spontaneously in vitro from fragments of rous sarcoma virus (rsv) gag protein purified after expression in escherichia coli. the ca-nc fragment of gag was shown previously to assemble into hollow cylinders (s. campbell and v. m. vogt, j. virol. 69:6487-6497, 1995). we have now extended ... | 1997 | 9151833 |
| treatment of respiratory syncytial virus bronchiolitis: 1995 poll of members of the european society for paediatric infectious diseases. | among the lower respiratory tract infections during infancy requiring hospitalization, respiratory syncytial virus (rsv) bronchiolitis is the most frequent disease entity. nevertheless treatment remains controversial. | 1997 | 9154540 |
| ribavirin therapy in bone marrow transplant recipients with viral respiratory tract infections. | treatment with ribavirin was instituted in 12 allogeneic and one autologous bone marrow transplant (bmt) recipients with proven respiratory syncytial virus (rsv), influenza b virus or parainfluenza virus infections. rsv was diagnosed in six cases, influenza b virus in four and parainfluenza virus in three patients. ribavirin was given orally or intravenously (15-20 mg/kg/day in three divided doses) and in nine cases with the addition of ribavirin inhalations (6 g/day). three patients required ve ... | 1997 | 9156264 |
| episomal expression of wild-type cftr corrects camp-dependent chloride transport in respiratory epithelial cells. | the isolation of the gene responsible for the cl- ion transport defect in cystic fibrosis (cf) has provided important information about the relationship between the disease pathology and the underlying genetic and biochemical mechanisms. in addition, new areas of investigation and therapy are now possible. most notably, the isolation of the cf gene, the cystic fibrosis transmembrane conductance regulator (cftr) has been led to the development of different gene therapy strategies. to circumvent p ... | 1996 | 9156804 |