Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| bacterial characteristics determining the potential host range of bacteriophage t4. | many members of the enterobacteriaceae, but not other gram-negative organisms, apparently carry a specific recognition site for the t4 tail tube on their cytoplasmic membranes. | 1976 | 956114 |
| control of mutation frequency by bacteriophage t4 dna polymerase. i. the cb120 antimutator dna polymerase is defective in strand displacement. | the ts cb1200 (antimutator) mutation in bacteriophage t4 dna polymerase increases the accuracy of dna replication since it results in a decrease in the frequency of mutations in other phage genes. the cb120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand, even in the presence of the t4 dna unwinding protein (gene 32-protein). the ratio of nucleotides turn ... | 1976 | 956182 |
| control of mutation frequency by bacteriophage t4 dna polymerase. ii. accuracy of nucleotide selection by the l88 mutator, cb120 antimutator, and wild type phage t4 dna polymerases. | the accuracy of nucleotide selection by wild type, l88 mutator, and cb120 antimutator t4 dna polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the dna-dependent conversion of deoxynucleoside triphosphate to monophosphate. the increased accuracy of the cb120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(da)-poly(dt) as template which is only 10 to 30% of that ... | 1976 | 956183 |
| plasma thyrotropin, thyroxine, and triiodothyronine relationships in man. | the physiologic relationships of plasma tsh, t4 and t3 levels measured every 20 min in seven healthy young men and one healthy young woman have been investigated. a nocturnal tsh surge was observed in all subjects on both nights of the 36-48 h baseline observation period. in males the maximum plasma tsh value occurred at 2300 h. the mean peak tsh level was 2.0 +/- 0.3 (se) muu/ml compared with a mean of 1.3 +/- 0.9 muu/ml for the entire baseline records of the 8 subjects. the effect of iv infusi ... | 1976 | 956341 |
| thyroid-pituitary feedback during iodine repletion. | the changes in serum triiodothyronine (t3), thyroxine (t4), and thyrotropin (tsh) were measured during iodine repletion in a woman who was severely iodine-deficient because of a congenital iodide-trapping defect. serum t3 became detectable 12 h after iodine was begun and reached 68 ng/dl, a level within the normal range, by 36 h. it rose progressively during the first 9 days reaching a supra-normal level (200 ng/dl) and then fell slowly to 130 ng/dl. serum t4 was not detected (less than 1 mug/dl ... | 1976 | 956345 |
| thyroid function in neonatal hypothyroidism. | various aspects of the thyroid function have been measured in 28 cases of neonatal hypothyroidism detected by means of the quebec screening program for metabolic diseases. in all instances the t4 value in the blood of filter paper spot was below 2 sd of the mean of the day, averaging 0.39 +/- 0.04 ng/40 mul (mean +/- sem) of eluted blood. the t4 value of a second similar sample averaged 0.22 +/- 0.04 ng/mul of eluted blood; this value was significantly lower than the first one. the serum t4 conc ... | 1976 | 956995 |
| tsh measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism. | a sensitive radioimmunoassay for the measurement of tsh in the eluate of blood spotted on filter paper has been developed. the method is consistently sensitive to 0.1 to 0.25 muu of tsh and enables the detection of values equivalent to 6 to 15 muu/ml of serum. the measurement of tsh in the filter paper spot in all infants with low filter paper spot t4 has permitted rapid confirmation of 10 cases of neonatal hypothyroidism. however, cases of hypothalamic hypothyroidism with low or normal filter p ... | 1976 | 956996 |
| head length determination in bacteriophage t4: the role of the core protein p22. | 1976 | 957424 | |
| mutator mutations in bacteriophage t4 gene 32 (dna unwinding protein). | bacteriophage t4 gene 32 encodes a dna unwinding protein required for dna replication, repair, and recombination. gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. | 1976 | 957480 |
| utilization of early promotors in mutant far p85 of bacteriophage t4. | we show that farp85 is a recessive mutant of t4 incapable of activating the delayed early promotors for genes 43 and 45 and that the farp85 mutation is in the same complementation group as the ts g1 mutation, which is located in the "modifier of transcription" (mot) gene. | 1976 | 957485 |
| plasma catecholamine concentrations in hyperthyroidism and hypothyroidism. | using a modification of the fluorometric method of anton and sayre, we have measured the plasma epinephrine (e) and norepinephrine (ne) concentrations in patients with thyroid dysfunction. there was no significant difference in plasma e in hyperthyroid or hypothyroid subjects, the values being similar to those observed in normal subjects. there was a striking relationship between age and plasma ne in the euthyroid individuals (r = 0.685, p less than 0.001, n = 41). observed plasma ne concentrati ... | 1976 | 958003 |
| studies on obesity. iii. effect of triiodothyronine (t3) on thyroglobulin autoantibodies in euthyroid obese subjects. | effect of t3 therapy on tanned red cell agglutinating thyroglobulin (trc-tg) antibodies in 10 obese subjects without apparent thyroid disease was investigated. six other obese subjects without thyroid dysfunction and of approximately the same mean age who also had circulating trc-tg antibodies served as control subjects and were untreated. in vitro thyroid tests (tsh, total and free t4) performed before t3 therapy, as well as clinical examination, showed thyroid function to be normal in all subj ... | 1976 | 958004 |
| [thyroid hormones and thyrotropin in liver and kidney insufficiency]. | in 22 patients with hepatic or renal insufficiency the serum concentrations of trijodothyronin, thyroxine and thyrotropin and also the t4-binding capacity of tbg were determined. the mean serum t3 concentration was found to be significantly lower in patients with hepatic coma when compared with euthyroid subjects. in the cases of renal insufficiency the serum t3 concentrations were in the normal range. due to hormone loss through dialysis however, the mean value of the t3 concentrations was slig ... | 1976 | 958900 |
| pathways of dna repair in t4 phage. ii. sedimentation analysis of intracellular dna in repair-defective mutants. | 1976 | 960557 | |
| subcellular membrane fatty acids of rat heart after cold acclimation or thyroxine. | relative percent changes in fatty acid (fa) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. rats in both environmental groups received thyroxine (t4) (1 mg/kg per day) or propylthiouracil (ptu) (5 mg/rat per day) for 10 days prior to sacrifice. mitochondrial total unsaturated fatty acids (tufa) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals recei ... | 1976 | 961919 |
| effects of 3'-fluorothymidine, nalidixic acid, and mitomycin c on temperature-sensitive mutants of the dna polymerase of phage t4. | the temperature-sensitive dna polymerase mutator mutants a58 and l98 are less inhibited by 3'-fluorothymidine than the t4 wild type and the antimutator mutant cb121, in consequence of the assumed differences between the polymerase-associated exonuclease activities. this interpretation is confirmed by results with nalidixic acid and mitomycin c. the use of systems of different temperature-sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) st ... | 1976 | 969568 |
| goiter formation following prostaglandin administration in rats. | prostaglandins (pge1 and pge2) induced a hyperplastic microfollicular goiter with a high radioiodine (131i) thyroid uptake, increased endocytosis, a heavy autoradiographic (125i) reaction, and a moderate increase of thyroid hormones (t4, t3), thyroxine-binding globulin (tgb), and thyrotropin (tsh) concentrations in adult rats. ultrastructurally, both prostaglandins (e1 and e2) markedly stimulated the thyroid cell activity and increased the number of pseudopodia, the size of colloid and dense gra ... | 1976 | 970439 |
| structure of t4 polyheads. ii. a pathway of polyhead transformation as a model for t4 capsid maturation. | 1976 | 972397 | |
| replication of t4 dna in vitro. ii. assay system for and some properties of gene products required for t4 dna replication. | [3h]dttp was not incorporated into t4 dna in the in vitro system for t4 dna replication when the system was prepared from cells infected with t4 amber mutants defective in dna replication. [3h]dttp incorporation was resumed by adding the missing gene product to the defective system. dna replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. by using this in vitro complementation system, molecular weights of gene 41, 43, 44 ... | 1976 | 972431 |
| the thyroidal response to chronic goitrogenic stimulation and the persistence of effects of early goitrogenic stimulation. | the effects of overstimulation of the thyroid gland induced by the feeding or rapeseed meal or thiouracil during the growing period persisted for as long as 75 weeks after withdrawal of the source of goitrogen from the diet. thyroid weight, thyroid iodine content and radioiodine uptake were all increased in birds which had received goitrogen during the growing period. follicle diameter was greater and the height of epithelial cells was lower than in control birds. the thyroid changes in these bi ... | 1976 | 974885 |
| optimization of t assay: a model study. | factors involved in the optimization of competitive binding radioassays have been analyzed using a thyroxine (t4) radioassay as a model system. the effects of kinetic errors are minimized by the use of the lowest possible incubation temperature for the separation step and by the use of labeled t4 of high purity (which is not consistently available from commerical sources). the use of labeled t4 containing significant quantities of labeled triiodothyronine (t3) can lead to a marked increase in bi ... | 1976 | 975571 |
| effect of fatty acids on thyroid function tests in vitro and in vivo. | addition of long-chain fatty acids to serum increased thyroxine (t4), measured by a competitive protein binding assay, and triiodothyronine (t3) uptake by sephadex or resin (t3u tests). this is compatible with the assumption that fatty acids compete with thyroxine for binding sites on t4-binding proteins. when equimolar concentrations of various saturated and unsaturated fatty acids were added to serum it was observed that the effectiveness in raising tests based on protein binding of thyroid ho ... | 1976 | 975583 |
| pharmacological influences on t4 to t3 conversion in rat liver. | five groups of 8 rats were treated with normal diet, diphenylhydantoin, dexamethasone, thyroxine (t4) and phenobarbital respectively for 14 days. after this period plasma triiodothyronine (t3), t4 and reverse t3 (rt3) concentrations as well as the t4 to t3 converting activity in the liver homogenates were determined. plasma t3 was low after dexamethasone, t4 and phenobarbital. plasma t4 was low after dexamethasone and elevated after t4. rt3 plasma concentrations were at the lower limit of detect ... | 1976 | 975586 |
| pituitary thyrotropin (tsh) rebound phenomenon and kinetics of secretion in the goitrous rat: differential effects of thyroxine on synthesis and release of tsh. | the protein metabolism and [3h]-uridine uptake of thyroid and adenohypophysis and the kinetics of pituitary tsh rebound (ptr) were studied in goitrous female rats (fed propylthiouracil, ptu: for 7-12 weeks) following single, iv injections of l-thyroxine (t4: 0.8 to 200 mug). goitrogenesis was associated with reduced protein concentration and enhanced uptake of [3h] uridine in both glands. plasma levels of tsh were invariably elevated but stores in the adenohypophysis were consistently reduced. s ... | 1976 | 976195 |
| compensatory thyroid hypertrophy after hemithyroidectomy in rats. | thyroid enlargement occurs in association with a variety of circumstances characterized by an impaired capacity of the gland to secrete adequate amounts of hormone. to elucidate the factors responsible for such compensatory thyroid growth, particularly the role of tsh, we have observed the response of the serum tsh, t3 and t4 concentrations following hemithyroidectomy in the rat, and have attempted to correlate changes in these functions with changes in the weight and histology of the thyroid re ... | 1976 | 976198 |
| a comparison of the effect of propylthiouracil on the respiration of thyroid slices from humans, chickens and rats. | 1. the qo2 of thyroid slices from hyperthyroid patients treated with propylthiouracil (ptu) was 140% that of slices from euthyroid patients. similar results were obtained using thyroid slices from ptu fed and control chickens. 2. ptu feeding caused thyroid hyperplasia, increased qo2 more than could be accounted for by the hyperplasia but neither increased qo2 of muscle, cerebral cortex or spleen nor altered serum t4 levels. 3. ptu in drinking water increased the size and vascularity of thyroids ... | 1976 | 976744 |
| dose-response of prolactin and thyrotropin to n3im-methyl-thyrotropin releasing hormone in euthyroid men. | the synthetic n3im-methyl analogue of thyrotropin releasing hormone (methyl-trh) was administered intravenously to 15 euthyroid men, ages 36-62, in graded doses from 6.25 mug to 500 mug in order to establish the range of response of prolactin (prl), tsh, t3 and t4 to various doses of methyl-trh. there was a dose-related rise in serum tsh, prl, t3, and t4 which gave a nearly linear relationship when the integrated area of response was used as an index of response to the various doses of methyl-tr ... | 1976 | 977724 |
| radioimmunoassay kits for thyroid hormones. | characteristics of the t4 and t3 radioimmunoassay kits that are commercially available in the uk are described. useful comparative data and criticism are given about many of these kits. some unacceptable variation in accuracy is demonstrated. | 1976 | 977766 |
| serum triiodothyronine determination in clinical use. | two radioimmunassays for the determination of serum triiodothyronine (t3) were developed. the assay of t3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of t3 in serum from 117 patients requiring assessment of their thyroid status. in 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum t3 concentration was 1-92 (actual range 0 ... | 1976 | 977774 |
| studies of the structure of the t4 bacteriophage tail sheath. i. the recovery of three-dimensional structural information from the extended sheath. | 1976 | 978722 | |
| role of genes 46 and 47 in bacteriophage t4 reproduction. iii. formation of joint molecules in biparental recombination. | 1976 | 978724 | |
| [successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)]. | continuous plasmapheresis with the ibm blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. the patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. although 633 mug thyroxine (t4) and 13.6 mug triiodothyronine (t3) were removed with the 51 plasma, no fall occurred in total serum t4 and t3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascula ... | 1976 | 979079 |
| the dependence of hno2 mutagenesis in phage t4 on ligase and the lack of dependence of 2ap mutagenesis on repair functions. | a temperature sensitive ligase allele of phage t4 reduced or eliminated hno2 induced reversion of am mutants. since at the temperatures used, the ligase mutant is defective in the repair of some types of lethal lesions (i.e., uv, mms and ems induced lesions) these results indicate that hno2 mutagenesis may occur through a ligase dependent repair pathway. in contrast, 2ap induced mutation was not inhibited by mutants defective in the gene 30 ligase or in genes 32, 39, 41, 42, 44, 45, 46, 47, 49, ... | 1976 | 979960 |
| serum t3 and t4 levels in sick children. | 1976 | 980611 | |
| freezing injury in bacteriophage t4. | 1976 | 981243 | |
| radioimmunoassay for serum thyroxine. | a new procedure for radioimmunoassay (ria) was applied to determine the serum concentration of thyroxine (t4). mixtures of t4-methylester hydrochloride-bovine serum albumin complex and complete freund's adjuvant were injected to rabbits to get them immunized. specific anti-t4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. ria for t4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125- ... | 1976 | 982432 |
| morphologically recognizable markers for scanning immunoelectron microscopy. ii. an indirect method using t4 and tmv. | 1976 | 982828 | |
| recurrent goiter, hyperthyroidism, galactorrhea and amenorrhea due to a thyrotropin and prolactin-producing pituitary tumor. | a 22-year-old woman with recurrent goiter, hyperthyroidism, galactorrhea, and amenorrhea due to a pituitary tumor is described. she had been treated surgically twice for recurrent goiter with tracheal compression. despite clinical signs of hyperthyroidism and slightly elevated plasma thyroid hormone levels (t4: 11 mug/dl; t3: 189 ng/dl), without thyroid hormone replacement therapy the basal tsh level was elevated up to 23 muu/ml and could not be suppressed by exogenous thyroid hormones: even whe ... | 1976 | 985824 |
| diural tsh variations in hypothyroidism. | there is a circadian variation in serum tsh in euthyroid subjects. a similar diurnal variation has been demonstrated in patients with hypothyroidism. in the present study the 24-hour pattern of serum tsh was investigated in eight patients with hypothyroidism of varying severity and in five hypothyroid patients treated with thyroxine (t4). there was a circadian variation in serum tsh in patients with hypothyroidism of moderate degree, and in patients treated for severe hypothyrodism with thyroxin ... | 1976 | 985825 |
| [tsh stimulation test using serum t3 and t4 as indicators]. | 1976 | 986885 | |
| [reproducibility of emesis by orally administrated copper sulfate in cats]. | adult cats were administered oral threshold doses of copper sulfate every week. as the cats vomited in 70 out out 80 cases, the reproducibility was 88%. peripheral vomiting threshold dose was 40 mg/head or less, while the threshold dose for oral copper sulfate emesis after t4 transection and vagotomy was more than 160 mg/head. the following method is thus proposed for application in evaluating an antimetic for oral copper sulfate. adult cats are to be given the emetic once a week. the threshold ... | 1976 | 986987 |
| [influencing of the thyroid hormone system by long-term treatment with 5-5 diphenylhydantion in children and adolescens (author's transl)]. | in the context of anticonvulsive maintenance therapy in epileptic children and adolescents, 5-5-diphenylhydantoin leads to a statistically significant decrease in total thyroxin (t4), protein-bound iodine (pbj) and free thyroxin (ft4). the consequences of dph therapy are based on the direct and indirect influence of dph on the thyroid hormone system. dph competitively pushes the t4 out of its plasma-protein bond. the lowering of t4, pbj and finally also ft4 can be traced back to an indirect infl ... | 1976 | 987507 |
| [analysis of thyroid hormones in serum and urine by mass fragmentography using gc-ms]. | the first successful analysis of iodine compounds in serum and urine by mass fragmentography using gc-ms combined system was performed. the equipment used was shimadzu lkb 9000 gc-ms (mid-pm). the tmsi derivatives of the compounds were analyzed by gc-ms system equipped with a 3 ft x 3 mm column packed with 1% ov-1 and the temperature was programmed from 200 degrees to 320 degrees c at 10 degrees c/min. the mass specturm showed molecular ions at m/e 523, 649, 741, 867 and 993 which correspond to ... | 1976 | 987938 |
| [the relationship between the timing of the korotokoff sound (qkd) and the serum thyroid hormone concentrations]. | the interval between the onsets of the qrs complex and of the brachial korotokoff sounds at the diastolic pressure was termed qkd and it was already known that qkd was shortend in hyperthyroidism and prolongad in hypothyroidism distinctly. in the present study, simultaneous measurements of qkd, serum thyroxine (t4) and triiodothyronine (t3) concentrations, free t4 index (ft4i) and free t3 index (ft3i) were undertaken and attempts were made to examine the possible correlation of qkd to these para ... | 1976 | 987939 |
| sensitivity to lithium in treated graves' disease: effects on serum t4, t3 and reverse t3. | seven patients judged to be euthyroid following treatment of diffuse toxic goiter were studied to determine if they were susceptible to lithium induced hypothyroidism. lithium carbonate was administered for 4-7 weeks in a dosage (900 mg/day) which maintained serum lithium levels between 0.5-1.0 meq/l. blood was obtained weekly for the determination of serum 3,5,3'-triiodothyronine (t3), thyroxine (t4), 3,3',5'-triiodo-l-thyronine (reverse t3, rt3) and thyrotropin (tsh). values observed during li ... | 1976 | 989049 |
| t4 and t3 release from the perfused canine thyroid isolated in situ. | a method for once-through perfusion of the canine thyroid isolated in situ is described. the perfusion medium was a modified krebs ringer buffer with 4% dextran added. in 4 control experiments of the t4 and t3 concentratios in effluent were stable or slightly falling during 3 h perfusion. there were no significant alterations in the t4/t3 ratio in the effluent during these experiments. a 10-min infusion of bovine tsh (1 mu/ml) caused an increase in the release of t4 and t3 after 15-25 min. the t ... | 1976 | 989219 |
| an evaluation of lithium as an adjunct to carbimazole treatment in acute thyrotoxicosis. | the rate of control of thyrotoxicosis during the first 2 weeks of treatment was documented in 63 patients. twenty-three patients received carbimazole 40 mg plus lithium carbonate 750 mg daily and a comparable group of 20 patients were given carbimazole 40 mg plus potassium iodide 120 mg daily. in the lithium treated patients the mean percentage fall of serum t4 after 2 weeks treatment was 49% and the fall in serum t3 57%. the results were similar in the iodide treated patients; the mean falls in ... | 1976 | 989225 |
| [serum t4 and t3 levels during administration of lugol's solution in basedow's disease]. | 1976 | 989471 | |
| concentration of thyroid hormone in skeletal muscle. | 1. a modified method for the determination of thyroid hormone levels in muscle is described. 2. the levels of t4 and t3 in skeletal muscle tissue of rats lie in the same range. 3. the level of t4 in plasma is about a hundred times higher than that of t3. 4. to remove plasma hormones from the tissue in which hormone levels are to be determined, the animals should be perfused with saline before tissue samples are taken. | 1976 | 989664 |
| an inter-laboratory comparison of total serum triiodothyronine determination. | l-triiodothyronine (t3) standards and an unknown serum were prepared and distributed to 18 different laboratories. t3 was determined on the standards and both t3 and t4 were assayed on the serum. the t3 and t4 results varied widely with a mean variance of 36 and 23%, respectively. there was, however, a positive correlation between the t3 values obtained within each laboratory and results of the unknown serum. thus, while the data of each laboratory is internally consistent, the data between labo ... | 1976 | 989996 |
| changes in serum thyroxine, triiodothyronine, and thyrotropin induced by lithium in normal subjects and in rats. | serum thyroxine (t4), triiodothyronine (t3), and thyrotropin (tsh) were measured in 4 normal subjects before, during, and 2 wk after administration of lithium carbonate (1,200 mg/day) for 6 wk. all subjects developed thyroid enlargement associated with increase in tsh. serum t3 and t4 did not change significantly. in 3 hypothyroid maintained on thyroxine replacement, lithium administration did not alter circulating levels of t4 or t3, suggesting no effect of lithium on the extrathyroidal metabol ... | 1976 | 991540 |
| secretion of a bone resorbing factor by chick thyroid glands in organ culture. | the ability of the thyroid gland to secrete a bone resorbing factor in vitro was studied using glands obtained from 20-day-old chick embryos. the glands were incubated in a modified bgj medium containing 1 mg/ml bovine serum albumin under 5% co2-40% o2 at 37 c. the culture media were assayed in vitro by measuring the stimulation of the release of previously incorporated 45ca from cultured 19-day fetal rat bone shafts over a 48 h period. the glands secreted a stimulator of bone resorption which d ... | 1976 | 991827 |
| determination of triiodothyronine in red blood cells by radioimmunoassay. | this study was undertaken to determine t3 content in red cells by radioimmunoassay. t3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell t3 content could be determined directly by radioimmunoassay of the lysate. after hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. the red-cell t3 content was expressed as ng/ml of red-cell volume. the normal t3 range in red cells was 0.20-0.45 ng/ml, and the mean+/-s ... | 1976 | 991832 |
| [suppression of dna-ligase deficiency and selective breeding of mutants and recombinants of t4 phage with phenotype rii]. | a number of methods which greatly simplify the introduction of frame shift mutations in the limited segment of the phage t4 rii genes were developed for studying genetical recombination between closely linked markers. these methods enable to construct multiple rii mutants with relatively small expenditure of labour. suppression of gene 30 deficiency by rii mutations has been studied for a variety of conditions. the results obtained indicate that riib polypeptide is involved in two different acti ... | 1976 | 992368 |
| dna "melting" proteins. iv. fluorescence measurements of binding parameters for bacteriophage t4 gene 32-protein to mono-, oligo-, and polynucleotides. | the quenching of the intrinsic tryptophan fluorescence of t4-coded gene 32-protein on binding to nucleotide ligands, which was described in the preceding paper, is here exploited to measure thermodynamic parameters of the single-stranded nucleic acid-gene 32-protein interaction. it is shown that binding of small ligands follows a single site binding isotherm, with association constants increasing from approximately 20 m-1 for phosphate, to approximately 10(3) m for ribose or deoxyribose 5'-phosp ... | 1976 | 993212 |
| visual failure during replacement therapy in primary hypothyroidism with pituitary enlargement. | a 34-year-old woman with longstanding untreated thyroprivic hypothyroidism and pituitary enlargement is reported here in whom visual failure coincided with thyroid hormone replacement. visual fields were normal after 30 years untreated hypothyroidism, but severe concentric field constriction developed during the first 6 months of therapy and was relieved by hypophysectomy. plasma tsh and prolactin remained elevated during 10 months replacement therapy, but both were suppressed by preoperative hy ... | 1976 | 993314 |
| an increase of plasma triiodothyronine concentration in man in a cold environment. | in an attempt to study an effect of cold on endocrine function in man, plasma thyroxine (t4), triiodothyronine (t3), tsh, and cortisol concentrations were studied in 24 healthy men who worked in a cold environment (4 to 6c) for 3 h for the culture of mushrooms and 56 healthy men who lived in cold environment in winter. for comparison, similar measurements were made on 47 university employees who lived in rooms with air-conditioning. in 24 adult men, acute exposure to cold for 3 h failed to affec ... | 1976 | 993317 |
| control of cell growth. iii. direct mitogenic effect of thyroid hormones on an estrogen-dependent rat pituitary tumor cell line. | a possible direct estrogen requirement for growth of gh3/c14 rat pituitary tumor cells was evaluated in culture medium supplemented with estrogen-depleted serum prepared by a 56 degree c charcoal extraction procedure, and with serum obtained from ovariectomized sheep and ovariectomized adrenalectomized sheep. growth of the gh3/c14 cells in culture medium in which the final estrogen concentration was 2 pg/ml or less was equal to growth in medium with normal serum and equal to growth in the presen ... | 1976 | 994217 |
| thyroid function and metabolic state in chronic renal failure. | thirty-eight patients with chronic renal insufficiency who were in a dialysis program underwent studies of thyroid function and metabolic status. mean values for serum total and free thyroxine (t4) concentrations and thyroxine-binding globulin capacity were within normal limits. although mean serum total triiodothyronine (t3) concentration was normal, 43% of the group had low serum t3 and 54% had low serum free t3 concentrations. serum thyrotrophin (tsh) concentrations were normal in all but fou ... | 1976 | 999108 |
| a comparison of the interference of fatty acids in the competitive binding radioassay and radioimmunoassay for serum t4. | 1976 | 1000836 | |
| [hybrids of phages lambda and t4]. | 1976 | 1001178 | |
| effects of growth hormone, thyroxine, and age on diaphragm muscle from dwarf mice. | the ability of gh in vitro to stimulate leucine incorporation into protein and the uptake of 2-aminoisobutyric acid (aib) and 3-o-methyl-glucose (3-omg) was studied in diaphragm muscle from dwarf (dw/dw) mice. ovine gh (25 mug/ml) significantly enhanced the rate of protein synthesis in muscle from untreated dwarfs. in contrast, gh usually failed to stimulate transport, although occasionally a small increase in aib uptake was seen. insulin (50 muu/ml) readily stimulated both aib transport and pro ... | 1976 | 1001246 |
| marker effects on reversion of t4rii mutants. | the frequencies of 2-aminopurine- and 5-bromouracil-induced a:t leads to g:c transitions were compared at nonsense sites throughout the rii region of bacteriophage t4. these frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. following 2ap treatment, they are high in amber (uag) and lower in opal (uga) codons than in allelic ochre (uaa) codons. in general, 5bu-induced transitions are more frequent in both amber and opal codons than in th ... | 1976 | 1001876 |
| effects of propranolol on changes in heart rate, heart weight, kidney weight, urinary hydroxyproline and weight gain induced by large doses of thyroxine in the rat. | the effects of the beta-adrenergic receptor blocking agent, dl-propranolol, and of the antithyroid drug, carbimazole, upon some manifestations of thyroxine (t4)-induced changes in peripheral metabolism were studied in rats. propranolol lowered the heart rate, but did not alter the following changes induced by t4: increment in heart rate, increase in heart or kidney weight, increase in urinary hydroxyproline, decrease in body weight gain or increase in serum t4. carbimazole administration lowered ... | 1976 | 1003065 |
| bacteriophage t4 prehead proteinase. ii. its cleavage from the product of gene 21 and regulation in phage-infected cells. | 1976 | 1003460 | |
| stimulation of the synthesis of bacteriophage t4 gene 32 protein by ultraviolet light irradiation. | 1976 | 1003487 | |
| [long-term critical evaluation of hypothyroidism after subtotal thyroidectomy for thyrotoxicosis]. | a retrospective study was made of 66 cases in which subtotal thyroidectomy had been carried for hyperthyroid syndromes, mainly of basedow type, with a view to establishing a more careful selection of thyrotoxicosis candidates for surgery or other management. the reasons for the different findings and post-operation evaluation of performance are analysed in the light of the time of follow-up, mostly within 5 to 15 yr of surgery. clinical examination in the light of statistical diagnostic indices ... | 1976 | 1004766 |
| normal conversion of thyroxine to triiodothyronine in a subject with iodotyrosine-dehalogenase deficiency. | bmr, t1/2 of serum t4, and the conversion of t4 to t3 were normal in a patient with iodotyrosine-dehalogenase deficiency who had undergone total surgical thyroidectomy and had been maintained on 200 mug of oral t4 daily. it is concluded that the enzyme system for monodeiodination and conversion of t4 to t3 is different than iodotyrosine-dehalogenase. | 1976 | 1004933 |
| quantitative study of extrathyroid enzymatic conversion of t4 to t3 in man. | the authors present a quantitative study of the extrathyroid conversion process of t4 to t3 in 3 athyroid patients, on the basis of an original biochemical model and a rigorous mathematical treatment, utilizing the kinetic isotope method. the values of thor the constant of the reaction rate of conversion of t4 to t3 and of the t4 fraction that undergoes this process--serum concentrations of t4 and t2--were determined in two completely different ways: 1) the radiochromatographic method, and 2) ra ... | 1976 | 1006135 |
| ability to perform prolonged physical exercise in dogs after chronic treatment with thyroxine. | treatment of dogs with l-thyroxine (t4) for 7 or 21 days had no influence on their body temperature, blood la and na concentrations and muscle glycogen content at rest. only blood ffa and glucose concentrations were significantly higher than those before t4 administration. during exercise performed by t4-treated dogs considerably higher increases in rectal temperature as well as in blood ffa and la concentrations were found in comparison with control runs. plasma na level at the end of exercise ... | 1976 | 1007928 |
| [hyperthyroidism by autonomous metastasis of thyroid carcinoma (author's transl)]. | nine years after surgical ablation of a trabeculo-vesicular carcinoma of the tyroid, a patient developped bone and liver metastasis. she had clinical signs of thyrotoxicosis, clear increase of blood t3 and sligh increase of t4. the tsh secretion was blocked. exogenous tsh increased iodine uptake in the thyroid and not in the metastasis. after 2 doses of 120 mci of 131i, she became hypothyroid, the liver was normal and the scan revealed the disappearance of uptake in thyroid and in metastasis. su ... | 1976 | 1008508 |
| inhibition of conversion of thyroxine to triiodothyronine in patients with severe chronic illness. | many clinically euthyroid patients with severe, chronic, non-throidal illnesses (i.e. sick euthyroid patients) have very low circulating concentrations of total and absolute free triiodothyronine (t3), low-normal concentrations of total thyroxine (t4), elevated concentrations of absolute free t4, and circulating concentrations of thyrotrophin (tsh) that are either normal or subnormal. this study was undertaken to elucidate the mechanism of the low circulating t3 concentrations. the disappearance ... | 1976 | 1009671 |
| the radioimmunoassay of 3,3',5' - triiodothyronine (reverse t3) in unextracted human serum. | a specific, sensitive and simple double antibody radioimmunoassay for total serum 3,3',5'-triiodothyronine (reverse t3, rt3) in small volumes of unextracted human serum is described. high titre antisera were raised in rabbits using dl=rt3 or l-rt3 conjugated to bovine serum albumin. the selected antisera cross reacted less than 0-003% with triiodothyronine (t3) and 0-14% with thyroxine (t4). a stable high activity rt3 tracer was prepared by iodination of 3,3"-diiodo-l-thyronine by the chloramine ... | 1976 | 1009675 |
| measurement of serum 3,3',5'-(reverse) t3, with comments on its derivation. | a radioimmunoassay for the measurement of l-3,3',5'-triiodothyronine (reverse to, rt3) has been developed for use with unextracted serum. the highly specific antiserum showed no cross-reactivity with l-3,3'5-triiodothyronine (t3) or tetradiodothyroacetic acid (t4a) and cross-reaction with l-thyroxine (t4) was low enough to be discounted for routine assay purposes. if a normal amount of rt3 was added to serum t4 cross-reactivity decreased considerably. serial dilutions of hyperthyroid sera gave d ... | 1976 | 1009677 |
| urinary thyroxine in rats fed various diets and in renal calcium stone-forming patients. | in rats fed with various diets as regard contents of magnesium, cholesterin, neutral fat, carbohydrates and proteins, urinary thyroxine (u-t4) appears low in general when compared with control chow, whereas renal calcifications and stones are absent only with the latter and the carbohydrate-rich diet. normalization of u-t4 is hampered by differences in urinary creatinine excretion and in individual weight gain. in male renal calcium stone patients (20--40 years) u-t4 also is lower than in age-ma ... | 1976 | 1009977 |
| [new lambdoid phages of escherichia coli. ii. comparison of several genetic characteristics with lambda phages]. | functions of some newly isolated lambdoid phages and phage lambda genes were compared by their ability to interact with unrelated phages and the product of the bacterial gene gro p. 19 of 23 lambdoid phages studied interfere with prophage p2, that points out the presence of functionally active genes, essential for spi+ phenotype in their genomes. the development of 4 lambdoid phages with spi- phenotype is independent on the prophage p2 presence. most of lambdoid phages show the reduced growth ab ... | 1976 | 1010327 |
| rice embryo cell-free system for the synthesis of t4 phage proteins. | 1976 | 1010575 | |
| assessment of thyroid hormone assays. | four techniques for estimating serum t4 and three for estimating serum t3 have been investigated and found to be satisfactory in routine use. normal ranges for each techniques have been established. estimation of serum t3 by the commerical kits tested appears to have a high discriminant value in the diagnosis of hyperthyroidism, although the diagnostic definition used inevitably enhances the apparent sensitivity of these techniques. estimation of serum t4 will identify the majority of patients w ... | 1976 | 1010875 |
| [simultaneous radioimmunassay for urinary thyroxine (t4) and triioldothyronine (t3) (author's transl)]. | a radioimmunoassay for the determination of thyroxine (t4) and triiodothyronine (t3) in urine was developed. the extraction of a sample, the incubations with t3-und subsequently t4-antibody and the elutions of the respective bound fractions are performed all on the same sephadex column. this principle can be applied to as many as 120 simultaneous determinations. the interassay coefficients of variation were 20.1% for t3 and 10.6% for t4, respectively. the recovery of standards added to urine was ... | 1976 | 1010989 |
| [need of thyroxine in chronic effects of growth hormone (gh) on phosphorus and calcium metabolism of female adult rat (author's transl)]. | the object of the present work was to determine the part played by thyroxine (t4) in chronic effects of gh on phosphocalcium metabolism. therefore, we used hypophysectomized-thyroparathyroidectomized female rats. the results were that: 1. repeated daily administration of gh to female rats which had been operated on was not followed by the hyperphosphatemia classically observed in normal animals. gh always decreased urinary calcium and phosphorus excretion, indicating a direct renal effect of thi ... | 1976 | 1011167 |
| [twice daily fractioning : application in the irradiation of severe skin neoplasms (author's tranls)]. | 14 very extensive skin cancer (t4) were treated with twice daily x-ray therapy (5 days per week, total dose 6,000 to 8,000 rads). 13 successful results were seen with follow-up for more than 14 months. | 1976 | 1011190 |
| screening thyrometabolic disorders using total serum thyroxine level. | the results of in-vitro thyroid function tests and clinical details of 1,517 patients are reviewed, and the total serum thyroxine levels and the free thyroxine indices are compared in terms of their diagnostic value. only 3-7% of the patients investigated had a total serum thyroxine level (t4) that did not correlate with the free thyroxine index. we believe that the evidence is strong enough to suggest that estimation of the t4 is an adequate screening test for thyrometabolic disease, and that p ... | 1976 | 1012127 |
| effect of neighboring nucleotide sequences on suppression efficiency in amber mutants of t4 phage lysozyme. | variations in suppression efficiency were observed among nonsense mutations at different locations within the lysozyme gene (e) of t4 phage. the present experiments using three amber mutants in lysozyme gene indicate such variations presumably depend upon the base sequences neighboring to the nonsense mutations. | 1976 | 1012265 |
| roentgen findings in fractures of the vertebral column in childhood examination of 35 patients and its results. | in the past 10 years we examined 35 children with fractures of the spine. the most important cause was an injury by a fall from a tree or a climbing stage (23 cases). traffic accidents or other direct trauma was the cause in 10 patients. two children had tetanus, the ages of the children range from 2 to 12 years. clinical symptoms may be diagnostic of vertebral trauma, but quite often symptoms are insignificant or atypical. we detected fractures in every vertebra of the thoracic and lumbar part ... | 1976 | 1012792 |
| blood levels of 3,5,3'-triiodothyronine and thyroxine: differences between children, adults, and elderly subjects. | the serum levels of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) in children, adolescents, adults, and elderly subjects have been measured by radioimmunoassays. it was found that while the t4 levels were essentially equal in all age groups examined, the t3 levels were markedly different. in children and adolescents (1-15 years), high values were recorded; indeed, they exceeded the upper normal limit in adults (20-80 years). from the age of 20, the t3 levels remained unaltered until the age of ... | 1976 | 1015359 |
| an increase of liver uptake of thyroxine, an initial step of an increased fecal loss of thyroxine in response to propylthiouracil in rats. | propylthiouracil augmented fecal loss of thyroxine (t4), but methimazole was without effect in this respect in rats. in vitro uptake of labeled t4 by the liver was significantly enhanced by a small amount of propylthiouracil in the presence of dilute rat plasma. methimazole was without effect under comparable conditions. in contrast, propylthiouracil and methimazole failed to affect in vitro uptake of labeled t4 by the kidney and diaphragm in the presence of dilute rat plasma. since deiodination ... | 1976 | 1015911 |
| [new method of solid phase radioimmunologic determination of total serum thyroxine compared with the competitive technic (t4-test)]. | 1976 | 1017126 | |
| [in vitro thyroid diagnosis in surgery of the thyroid]. | after a brief outline of the physiology of the thyroid hormones and the laboratory tests measuring thyroid function, the dosage of normalised t4 (t4n) in patients suffering from various thyroid diseases and subjected to surgical operation is discussed. as the fundamental presupposition of thyroid surgery is that the operation is made in conditions of euthyroidism, the use of a quick and reliable preoperative test giving an exact evaluation of the patient's thyrometabolic conditions is necessary. ... | 1976 | 1021298 |
| electron-microscopic study of the serological affinity between the antigenic components of phages t4 and ddvi. | in an investigation of the antigenic fine structure of phages t4 and ddvi with the use of the neutralization reaction and electron-microscopic observation of the phage-antibody complexes, it has been possible to establish that the head of phage t4 consists of proteins which have antigenic determinants of two types: the first type is identical to the antigens of the head of phage ddvi, and the second type is apparently absent in phage ddvi. the phage ddvi head contains mostly determinants which a ... | 1976 | 1023046 |
| influence of antibodies against dna on the renaturation of dna. | the treatment of denatured t4 phage dna with antiserum for the dna of this phage, containing antibodies against glucosylated 5-hydroxymethylcytosine, decreases the ability of dna for renaturation. the greatest inhibiting activity is possessed by antiserum for t4 phage dna irradiated with uv light, which contains antibodies not only against glucosylated 5-hydroxymethylcytosine, but also against the usual nitrogen bases. antiserum against e. coli dna, containing antibodies to the usual nitrogen ba ... | 1976 | 1023048 |
| dna replication and head assembly in bacteriophage t4. | phage dna was accumulated in cells of e. coli b, infected with the phage t4dtslb3 (gene 42), without the synthesis of late proteins (in the presence of chloramphenicol). then (stage ii), chloramphenicol was removed and further replication of the phage dna suppressed with hydroxyurea and by simultaneously raising the temperature to 40 degrees. the media m9 or m9 with 1% amino acid were used; the times of addition of chloramphenicol and the hydroxyurea concentration were also varied. it was also s ... | 1976 | 1023049 |
| clinical study on early changes in thyroid function of hyperthyroidism treated with propylthiouracil and a relatively small dose of iodide. | in order to compare the acute effects of three kinds of antithyroid agents of iodide (i-), propylthiouracil (ptu) and ptu combined with iodide (ptu+i-) on thyroid function in hyperthyroid patients with diffuse goiter, serum concentrations of thyroxine (t4), triiodothyronine (t3), t3-resin sponge uptake (t3-ru) and free thyroxine index (ft4i) were employed as thyroid function parameters. in the group given iodine (1 mg/day) as iodinated-lecithine, the initial values of t4, t3, t3-ru and ft4i were ... | 1976 | 1024041 |
| [comparison of the diagnostic efficacy of various in vitro tests for the study of thyroid performance (author's transl)]. | discriminating analysis was employed in an evaluation of the diagnostic efficacy of individual in vitro tests of thyroid performance (t3 resin uptake, t4, fti, etr) and pb131i determination, and their combinations, when used for the differentiation of euthyroid, basedow hyperthyroid, hyperfunctioning autonomous adenoma, and hypothyroid cases. the results are discussed with reference to optimization of the data: radiation dose ratio. | 1976 | 1027046 |
| a rational approach to "in vitro" thyroid function testing. | a system of "in vitro" thyroid function testing is proposed whereby laboratory staff select the most appropriate screening test depending on the information supplied by the clinician. total serum triiodothyronine (t3) or thyroxine (t4) are used for screening as appropriate. in borderline cases, secondary tests are performed automatically according to a flow chart. this system improves efficiency and is cost effective, saving approximately 1,800 pounds annually in a laboratory handling about 5,00 ... | 1976 | 1030665 |
| [connections between inspiratory medullary neurons and phrenic or intercostal motoneurones (author's transl)]. | 10 the activity of 107 medullary inspiratory neurones has been recorded extracellularly in anesthetized cats (urethane-chloralose). according to their localization in the medulla and to their axonal pathways (tested by antidromic activation), these neurones were classified as: bulbo-spinal neurones (nbsi) which send their axons to the spinal cord; they are located in the dorsal or the ventral respiratory nucleus; propriobulbar neurons (npbi) whose axons are probably entirely located within the m ... | 1976 | 1030738 |
| neuroendocrine and electroencephalographic sleep changes due to acute amphetamine ingestion in human beings. | amphetamine, a clinically used sympathomimetic central-acting drug, was administered in spansule capsules in a blind schedule to 8 normal obse volunteers in a daily (8 a.m.) single 15 dose for 7 days. the study, conducted in the metabolic ward, included two 7 day placebo periods (pre- and post-drug). during the 1st placebo period, all subjects exhibited within the 1st 2 h of sleep a clear and significant nocturnal increase of growth hormone (gh) closely related with sleep stages 3 and 4. thyrotr ... | 1976 | 1030786 |
| diagnosis of prolactin-secreting pituitary microadenoma. | four women with secondary amenorrhea associated with hyperprolactinemia were studied. baseline hormonal evaluation, including serum fsh, serum lh, tsh, t3, t4, and plasma cortisols were normal. plain sella turcia x-rays were also normal. prolactin-secreting pituitary microadenomas were found in all of the patients only after further diagnostic studies were done. these studies included polytomography of the sella turcia, dynamic pituitary testing of growth hormone reserve, acth reserve, gonadotro ... | 1976 | 1033671 |
| [distinction in clinical application of various thyroid hormone tests (t4, t3, t3 absorption test, and free t4 index)]. | 1976 | 1034522 | |
| [serum thyroxine determination with t4 ria kit]. | 1976 | 1034531 | |
| thyroid hormone response to varying doses of tsh. | fifty-five normal subjects were studied following intravenous injection of increasing doses of bovine thyrotropin (b-tsh) from 0.5 to 250 mu/kg. serum triiodothyronine (t3) and thyroxine (t4) were measured 1, 2, 3 and 4 h after the tsh injection. dose-related increments in serum t3 and t4 were demonstrated with doses of b-tsh = 2.5 mu/kg and a maximum response was obtained after approximately 100 mu/kg. the fractional increase in serum t3 was greater than in serum t4, but the ratio between the i ... | 1976 | 1036647 |