Publications

response to primary infection with herpesvirus saimiri in immunosuppressed juvenile and newborn squirrel monkeys.	immunosuppression of juvenile squirrel monkeys with combined azathioprine, prednisolone, and antilymphocyte globulin resulted in decreased antibody responses to viral antigens after primary infection with herpesvirus saimiri (hvs). the virus was repeatedly isolated from the oropharynx of immunosuppressed monkeys but not from untreated infected controls. thus immune factors are important in inhibiting shedding of hvs from the oropharynx. hvs could be isolated from the peripheral blood lymphocytes ...	1975	170204
design and construction of an apparatus for the growth of micro cell cultures on standard glass microscope slides and its application for screening large numbers of sera by the indirect fluorescent antibody technique.	a simple stainless-steel apparatus was designed to contain standard microscope slides on which were grown micro cell cultures in the form of 16 individual monolayers per slide. the application of this apparatus for the screening of serum samples by fluorescent antibody techniques is described.	1975	170316
studies on the adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes in the mouse. i. in vitro enhancement of antibody formation with normal spleen cells.	the adjuvant effect of bordetella pertussis vaccine (pv) on the antibody response to sheep erythrocytes (srbc) has been studied in vitro with the mishell-dutton immunization technique. the addition of pv to cultures of spleen cells obtained from normal non-immunized mice markedly enhanced the plaque-forming cell response to srbc. the greatest enhancement was evident at 24 hr of culture. pv was also shown to enhance the antibody response of spleen cells that had been depleted of either t lymphocy ...	1975	170337
immunosuppression by spleen cells from moloney leukemia. comparison of the suppressive effect on antibody response and on mitogen-induced response.	the inhibitory effect of cells from leukemic spleens on the immune functions of normal lymphocytes was studied. suppressor cells were obtained as the nonadherent fraction (na) from splenic tumors of mice infected with mulv-moloney. this fraction (na mulv- m) contained less than 10% membrane ig-positive (ig+) cells, 45 to 60% theta-positive cells (theta+) and 40 to 50% naught cells (theta-, ig-). similarly prepared fractions from normal control spleens (nac) containing 75 to 90% theta+cells and l ...	1975	170339
effect of adrenal hormones on thyroid secretion and thyroid hormones on adrenal secretion in the sheep.	1. previous work has shown that after stressful stimuli, sheep initially secrete increased amounts of thyroid hormone, at a time when adrenal secretion is also elevated. 2. this study was designed to evaluate (a) any short-term activation or inhibition of thyroid secretion by exogenous cortisol or acth administered in quantities comparable to those secreted after stress in sheep and (b) any short-term effect that exogenous thyroxine or triiodothyronine may have on the concentration of plasma cor ...	1975	170400
the effect of solanum malacoxylon on rachitic bone lesions in the rat.	the plant solanum malacoxylon (s.m.) is known to cause severe soft tissue calcifications in cattle and sheep and has recently become of special interest since it exerts biological actions which resemble those of vitamin d. in order to investigate whether s.m. is capable to improve the rachitic bone changes in vitamin d and phosphate deficient rats, a watery extract of 50, 100 and 200 mg s.m. was fed daily to these animals over a period of 10 days. the width of epiphyseal plates was compared afte ...	1975	170546
[serological screening of streptococci. comparison of 2 methods of slide agglutination, one with streptolysin alone, the other with several enzymes (streptozymes)].	using 100 sera randomly selected, the clinical characteristis of which were known, we compared two methods of rapid detection of streptococcal infections by slide agglutination. one was the classical latex test for detection of anti-streptolysin o, the other was the streptozyme test. the latter reagent detects the presence of 5 streptococcal antibodies, anti-streptolysin o, anti-streptokinase, anti-streptohyaluronidase, anti-desoxyribonuclease and anti-nicotinamide-adenine-dinucleotidase. it was ...	1975	170573
follicle-stimulating hormone stimulates estradiol-17beta synthesis in cultured sertoli cells.	sertoli cells isolated from testes of 20-day-old rats and maintained in primary culture synthesized estradiol-17beta [1,3,5(10)-estratriene-3,17beta-diol] (measured by specific radioimmunoassay) when testosterone (17beta-hydroxy-4-androsten-3-one) 0.5 mum, was added to the culture medium. no detectable estradiol synthesis occurred when cells were incubated in medium containing pregnenolone (3beta-hydroxypregn-5-en-20-one), 0.5 mum, or containing no added steroid substrate. follicle-stimulating h ...	1975	170613
intrauterine pressure wave form characteristics in hypocontractile labor before and after oxytocin administration.	the data demonstrate that the contractions of hypocontractile active labor and normal spontaneous labor are different in several measures in addition to maximal amplitude. furthermore, when the pathophysiology is corrected by the use of oxytocin, the contractions resemble those of normal spontaneous labor except in the maximal rate of tension development. our data tend to support the subcellular model of uterine contractility, although the incompleteness of these models limits interpretation.	1975	170827
effects of estrogens on systemic and regional circulations in normal and renal hypertensive sheep.	effects of estrogen administration on systemic and regional circulation were studied in normotensive and renal hypertensive, chronically instrumented nonpregnant and pregnant ewes. arterial pressure, cardiac output, and uterine, renal, superior mesenteric, and iliac blood flows, as well as uterine oxygen transfer, were monitored before and after intravenous administration of premarin or estradiol-17 beta. the results show that: (1) estrogen administration produces a marked decrease in uterine va ...	1975	170829
human pituitary growth hormone. intrinsic lipolytic activity on rabbit fat cells.		1975	170868
the influence of some fractions of egg yolk on the survival of ram spermatozoa at 5 degrees c.	ram spermatozoa were stored at 5 degrees c in diluents containing various fractions of egg yolk prepared by dialysis, ultrafiltration and ion-exchange chromatography. they survived storage best in the presence of components of egg yolk which were non-dialysable and were not filtered through membranes which retained substances of molecular weight greater than 100 000. the substances isolated in peak b of the ion-exchange chromatogram of whole egg yolk described by seideman et al. (1969) gave grea ...	1975	170902
preparation of lutropin with acetyl or acetimidinyl substituents on the amino groups of the beta-subunit.	the free amino groups in ovine lutropin beta subunit were acylated with acetic anhydride and methyl acetimidinate-hcl to produce the corresponding acetyl and acetimidinyl ovine lutropin beta derivative. these two derivatives recombined with ovine lutropin alpha as well as native ovine lutropin beta, but produced lutropin derivatives which were 33-50% less active than the ovine lutropin alpha + ovine lutropin beta in biological assays.	1975	170984
the role of adrenocortical hormones in acth-induced hypertension.		1975	171112
3,3',5'-triiodothyronine (reverse t3) and 3,3',5-triiodothyronine (t3) in fetal and adult sheep: studies of metabolic clearance rates, production rates, serum binding, and thyroidal content relative to thyroxine.	to examine the mechanism(s) responsible for high serum concentration of 3,3',5'-triiodothyronine (reverse t3, rt3) and low serum concentration of 3,3',5-triiodothyronine (t3) in the fetus, we studied metabolic clearance rates (mcr) and production rates (pr) of rt3, t3, and thyroxine (t4) in adult nonpregnant sheep and sheep fetuses in utero. the mean fetal mcr-rt3 was significantly lower than that in adult sheep, and the mean fetal pr-rt3 significantly higher. the mean fetal mcr-t3 was higher th ...	1975	171141
a prospective evaluation of heterophile and epstein-barr virus-specific igm antibody tests in clinical and subclinical infectious mononucleosis: specificity and sensitivity of the tests and persistence of antibody.	three heterophile antibody tests and a test specific for igm antibody to epstein-barr virus were evaluated during prospective studies of infectious mononucleosis. specificity was judged by the frequency of false-positive results in sera of known qualities taken before illness; except for two patients bled during early, unrecognized illnes,, titers of greater than or equal to 1:40 were detected in 12% by the absorbed sheep red cell test, in 6.7% by the absorbed horse red cell test, and in none by ...	1975	171321
the stereochemistry of enzymic reduction of 21-dehydrocortisol to cortisol.		1975	171488
oestrogen-induced progesterone receptor in human uterus.		1975	171501
magnetic resonance and kinetic studies of the mechanism of membrane-bound sodium and potassium ion- activated adenosine triphosphatase.	epr and water proton relaxation rate (1/t1) studies of partially (40%) and "fully" (90%) purified preparations of membrane-bound (na+ + k+) activated atpase from sheep kidney indicate one tight binding site for mn2+ per enzyme dimer, with a dissociation constant (kd = 0.88 mum) in agreement with the kinetically determined activator constant, identifying this mn2+-binding site as the active site of the atpase. competition studies indicate that mg2+ binds at this site with a dissociation constant ...	1975	171521
electron microscopy of cowdria ruminantium infected reticulo-endothelial cells of the mammalian host.	an electron microscopic study of structures suspected to represent a possible developmental cycle of cowdria ruminantium in reticulo-endothelial cells of mice and ruminants is reported. after infection dense bodies increase in size and undergo division to form fragmented dense bodies. these in turn apparently sub-divide and become organized to give rise to mature "organisms". in none of these structures do limiting membranes separate the parasitic inclusions from the host cell cytoplasm. present ...	1975	171605
experimental infection of lambs with an adenovirus isolated from sheep and related to bovine adenovirus type 2. i. virological studies.		1975	171937
solid-phase radioimmunoassays for quantitative antibody determination of clostridium perfringens type d epsilon toxin.	two reversed solid-phase radioimmunoassays were developed for quantitative determination of antibodies against clostridium perfringens type d epsilon toxin. 125i-labeled prototoxin was used in the bromoacetylcellulose-bound antibody method and in the antibody-coated tube method. the antibody values which can be detected by the assays are in the range of 0.004 iu/ml of investigated serum. the methods allow the screening investigation of large groups of vaccinated sheep in a rapid and inexpensive ...	1975	172010
[suitability of immune serums from different animal species for the preparation of conjugates against aujeszky's disease virus].	a comparison was made of immune serum from rabbits, sheeps, pigs and cattle. pure igg was prepared by the rivanol sulphate method and immune electrophoresis. optimum protein quality, conjugation time under various conditions and using two preparations of fluorescein isothiocyanate were studied. conjugate purified by deae-sephadex a-50 and concentrated to 1% protein by ultrafiltration was freeze-dried and then tested for activity and the degree of non-specific fluorescence. evaluation by yield of ...	1975	172039
[effect of trypsin and of the injection, technic on the immunogenic action of toxins from clostridium perfringens types d and e].		1975	172064
erythropoietin and the differentiation of red blood cells.	erythropoietin is the primary factor regulating red blood cell formation in mammals and some other animals. it has been purified from plasma derived from anemic sheep and from the urine of anemic patients and is a glycoprotein. the sheep hormone has a molecular weight of 46,000 and appears to consist of a single chain. the carbohydrate and amino acid compositions where known, are summarized, as are the known structural requirements for biological activity. its mode of action as the inducer of re ...	1975	172374
presence of plasma-membrane enzymes in bile and their partial purification by affinity chromatography with concanavalin a.		1975	172386
the estrogen cytosol receptor of female ovine pituitary.	the biochemical parameters of estrone and estradiol binding to the cytosol fraction of ovine anterior pituitary were investigated. when increasing amounts of [3h]estrone or [3h]estradiol were incubated with the 105,000 g fraction from the pituitary, both hormones bound to a receptor with the same apparent kd (mean +/- s.e., estrone = 1.40 +/- 0.30 x 10(-10) m, estradiol = 1.03 +/- 0.11 x 10(-10)m) and the same concentration of binding sites (estrone = 3.22 +/- 0.58 x 10(-14) moles/mg protein, es ...	1975	172395
viral replication and interferon production in fetal and adult ovine leukocytes and spleen cells.	peripheral blood leukocyte and spleen cell cultures derived from adult sheep and from third-trimester (107 to 145 days of gestation) and second-trimester (70 to 98 days of gestation) fetal lambs were examined for their ability to support viral replication and to produce interferon. bluetongue virus, herpesvirus hominis type 2, and chikungunya virus failed to replicate in either leukocyte or spleen cell cultures derived from adult ewes or in cultures from second- or third-trimester fetal lambs. s ...	1975	172452
a solid phase micro-radioimmunoassay to detect minute amounts of ig class specific anti-viral antibody in a mouse model system.	a simple and rapid micro-radioimmunoassay was developed to detect and quantitate class specific mouse anti-sendai virus antibodies. two different 125i-labelled indicator systems were studied. after incubation of test serum with antigen one system used 125i-rabbit anti-mouse igg (ria 1) and the second employed rabbit anti-mouse igg, iga or igm followed by 125i-sheep anti-rabbit immunoglobulin reagent (ria 2). the ria 2 method was adopted for routine use as it was more sensitive, gave better discr ...	1975	172559
comparative studies of purinergic nerves.	purinergic nerves supply the gastrointestinal tract of vertebrates, including fish, amphibians, reptiles and birds, as well as mammals. their cell bodies are located in auerbach's plexus and their axons extend in an anal direction before innervating mainly the circular muscle coat. in the stomach they are controlled by preganglionic cholinergic fibres of parasympathetic origin. they are involved in "receptive relaxation" of the stomach, "descending inhibition" in peristalsis and reflex relaxatio ...	1975	172588
the exchange of phosphatidylcholine and of phosphatidylinositol in sheep brain.		1975	172606
our present knowledge of the pathology of dementias.		1975	172773
cytopathology in viral diseases.		1975	172783
stimulation of normal lymphocytes with autologous lymphoid cell lines: properties of derived killer cells.	lymphocytes from normal adults, with or without serological signs of previous epstein-barr virus (ebv) infection, could be stimulated to proliferate and produce killer cells by incubation with autologous ebv-genome-positive lymphoid cell lines (lcls). the stimulated cells were most probably of t-cell origin, although at the peak of stimulation many of them lacked the sheep erythrocyte marker. direct effector-target cell contact was necessary for lysis to occur. the cytotoxicity of autologously s ...	1975	173017
blue tongue in wiltshire-horn sheep.		1975	173073
[adaptation and cultivation of the perego strain of ovine smallpox virus in tissue culture and an evaluation of its immunogenic properties].	the strain perego of the sheep pox virus, used for the production of the ovinized vaccine in this country, was adapted and cultured in tissue cultures of lamb testes. a total of ten passages in succession of the virus were performed with the manifestation of a characteristic cytopathic effect in the cultures. the titer of the virus was found to be within the range of 10(3.5)cpe50, for the initial passages, to 10(4.5)cpe50, for the final passages. the activity and harmlessness of the virus was te ...	1975	173075
[experimental transmission of lymphatic leucosis of sheep].		1975	173118
pathogenicity of a caprine herpesvirus.	a herpesvirus isolated from neonatal angora kids (capra hircus) with a relatively severe generalized infection was shown to be infective for adult goats as well as for kids. however, the virus lacked pathogenicity for either lambs or calves. although a nonreciprocating serologic overlap exists between the caprine virus and the antibody to bovine herpesvirus (bhv-1), results of cross immunity tests in calves indicated that the 2 viruses are immunologically distinct. on the basis of these findings ...	1975	173212
kinetics of neutralization of two strains of myxovirus parainfluenza 3 in the presence of complement and anti-gammaglobulin-serum.	using the kinetics of neutralization, it was established that two strains of myxovirus parainfluenza 3 of cattle and sheep origin which are immunologicaly identical have a different sensitivity to early neutralizing antibodies of reconvalescent calf sera. complement-dependent neutralizing antibodies, supplied by adding 5% guinea pig serum to the calf serum, appear earlier than the usual neutralizing antibodies. goat anti-cattle gammaglobulin serum decreases the neutralizing activity of early rec ...	1975	173251
the occurrence of vacuolated neurons in the brains of hamsters affected with subacute sclerosing encephalitis following measles or langat virus infection.	hamsters infected by the intranasal route with either hamster adapted langat virus passaged at least 10 times in hamsters and the hnt strain of measles virus passaged 149 times in hamsters, developed subacute sclerotic lesions in the pyriform cortex, and beginning from 2 months after infection, were accompanied by neuronal vacuolation with ballooning of the cytoplasm, especially in parts of the brain in close proximity of the sclerotic lesions. the vacuolated neurons resembled those seen in scra ...	1975	173381
granule enzymes of polymorphonuclear neutrophils: a phylogenetic comparison.	the functional significance of granule enzymes in polymorphonuclear leukocytes (pmn) is not fully understood because of the multiplicity of the enzymes and the rare occurrence of deficiencies in man. in order to select appropriate laboratory animals for functional studies, a phylogenetic comparison of enzyme levels in animal and human pmn was undertaken. neutrophils were obtained from a variety of laboratory animals and man; the activities of alkaline phosphatase, lysozyme, myeloperoxidase, and ...	1975	173439
depression of humoral and cell-mediated immune responses by coxsackieviruses in mice.	adult mice infected with coxsackieviruses a-15, b-1, b-2, b-4 and b-6 showed depressed antibody responses to unrelated antigens; mice infected with coxsackievirus b-3 developed reduced humoral and cell-mediated immune responses. these findings might have clinical and epidemiological implications.	1975	173571
potentiation of erythropoiesis in vitro by dexamethasone.	the effect of dexamethasone on erythropoiesis was examined in vitro. hematopoietic cells from 13-day mouse fetal livers were cultured for 48 h in the presence or absence of erythropoietin and erythroid colonies enumerated. colony formation occurring in cultures containing no added erythropoietin was inhibited by the incorporation of antierythropoietin antibody, suggesting that these colonies formed in response to endogenous hepatic erythropoietin. maximal colony formation was observed with 0.5 u ...	1976	173742
proceedings: pituitary and adrenal function in foetal sheep during the latter half of gestation.		1975	173774
cytoplasmic oestrogen receptor complex of female ovine pituitary: changes associated with the reproductive state and oestradiol treatment.	binding of oestrone and oestradiol to a pituitary cytosol receptor was studied in ovariectomized sheep during the breeding season, during anoestrus and after oestradiol treatment during anoestrus. all sheep were ovariectomized 2 weeks before removal of the anterior pituitary. oestrogen treatment consisted of the subcutaneous implantation of silastic capsules containing oestradiol-17beta 3 weeks before ovariectomy. association constants (ka) for oestrone and oestradiol and the concentration of bi ...	1975	173786
unique virus-related dna sequences in sheep progressive pneumonia lung.	hybridization studies with [3h]-cdna of progressive pneumonia, maedi and visna viruses demonstrate that lung dna from sheep afflicted with progressive interstitial pneumonia possesses virus-related sequences not present in normal sheep lung dna.	1975	173794
proceedings: acth and the induction of parturition in sheep.		1975	173835
the sheep ovary: regulation of steroidogenic, haemodynamic and structural changes in the largest follicle and adjacent tissue before ovulation.	important changes occur in the follicle that is destined to ovulate and also in the surrounding ovarian tissue of sheep during the 72 hr preceding ovulation. it is our purpose to describe the morphological and functional changes that take place in large follicles during the preovulatory period and to relate these to the local ovarian environment and general endocrine status prevailing at this time. a consideration of changes in the structure, blood supply and function of the ovaries in vivo, is ...	1975	173846
adrenal autotransplants in sheep: prostaglandin a1 is not steroidogenic.		1975	173926
synthesis of cholesterol esters in the plasma and liver of sheep.	a study was made with sheep on the formation in vitro of long chain fatty acid esters of cholesterol by the lecithin-cholesterol-acyltransferase system present in the plasma and the acyl coa-cholesterola cyltransferase system present in the liver. the rate of cholesterol esterification in the plasma was 0.024 mumoles/ml/hr. the relative pattern of fatty acids esterified during incubation of the plasma remained constant over the 8 hr period of incubation and was similar to the fatty acids in the ...	1975	173965
spontaneous tumours in young animals.		1975	174124
[anaerobic gastroenterotoxemia in lambs].		1975	174271
[immunobiological properties of strains of foot-and-mouth disease virus multiplying in cell cultures].		1975	174272
[sensitivity of cells of the transplanted sheep kidney line to foot-and-mouth disease virus].		1975	174273
immunoglobulins, antibodies and inhibitors of parainfluenza 3 virus in respiratory secretions of sheep.	virus neutralising and haemagglutination inhibiting (hi) activities were monitored in the serum, nasal secretions and tracheo-bronchial secretions of lambs infected with parainfluenza virus type 3 (pi3). hi activity was found in the secretions of both control and infected lambs, whereas neutralising antibody was found only in nasal secretions from infected lambs. fractionation revealed that most of the hi activity in the secretions was due to a large molecular weight protein which also inhibited ...	1975	174528
chemical modification of the tryptophan residue in adrenocorticotropin.	the single tryptophan residue in the pituitary hormone adrenocorticotropin was modified selectively by reaction with a variety of substituted o-nitrophenylsulfenyl chlorides. in addition to quantitative modification of the tryptophan residue, the reaction invariably resulted in partial oxidation of the methionine residue to the sulfoxide. the methionine sulfoxide derivative could be separated from the desired product by partition chromatography on sephadex g-50 in the solvent system 1-butanol-py ...	1976	174723
characterization of an inhibitor for luteinizing hormone receptor site binding.	an inhibitor for luteinizing hormone (lh) receptor site binding has been partially purified from aqueous extracts of ovaries from pseudo-pregnant or pregnant rats. the lh receptor binding inhibitor (lh-rbi) was heat-resistant, partially inactivated by trypsin or pronase digestion, and had a molecular weight of approximately 3800 daltons. the lh-rbi was not found in the ovary of mature non-pregnant rats or immature rats, nor was it found in testis or liver extracts. the lh-rbi strongly inhibited ...	1976	174892
identification of cholesterol in the receptor site for rickettsiae on sheep erythrocyte membranes.	cholesterol was identified as an essential component of the receptor on the sheep erythrocyte to which rickettsia prowazeki adsorbs before lysing the cell. erythrocytes or ghosts, derived by hypotonic lysis, were treated with proteolytic enzymes, sialidase, sulfhydryl reagents, and periodate without affecting their ability to adsorb rickettsiae. lipid extracts of ghosts and erythrocytes, on the other hand, contained receptor activity. fractionation of the lipid extracts by silicic acid column ch ...	1976	175013
[light microscopic, electron microscopic and enzyme histochemical study of cerebral cortex of cattle, sheep and goat].		1975	175114
pituitary regulation of human growth hormone binding sites in rat liver membranes.	we have studied the binding of 125i-human growth hormone (hgh) to crude 100,000 x g membrane preparations from rat liver, and have studied factors which might regulate the capacity and affinity of hgh binding sites. membrane preparations have livers of pregnant rats bound between 8% and 18% of the 125i-hgh initially added, and 70%-80% of that bound was displaced by 1 mug of unlabeled hgh. humans prolactin (hprl) displaced 125 i-hgh in a manner parallel to hgh itself but with about one-third the ...	1976	175242
[cytopathogenic changes in cell cultures under the effect of avian laryngotracheitis virus].		1975	175252
initiation of labor by intraamniotic cortisol instillation in prolonged human pregnancy.	the fetal adrenal cortex has recently been implicated in the etiology of postmaturity on the basis of low cortisol levels found in the plasma of postmature neonates. these findings suggested that labor could be initiated in prolonged pregnancy by iatrogenic elevation of fetal plasma cortisol. in the current investigation designed to test this hypothesis, 500 mg of hydrocortisone sodium succinate, dissolved in the accompanying diluent of bacteriostatic water to form 4 ml of solution, was injected ...	1976	175321
intrathyroidal amines in the regulation of thyroid activity.		1975	175428
[prostaglandins and reproduction].	the prostaglandins probably play an important role in the physiology of reproduction. in women as in men, they intervene at several levels. at the level of the ovary, the prostaglandins e may be modulators of the action of gonadostimulin on ovarian hormonogenesis, in close relationship with cyclic amp and, furthermore, represent an important biochemical intermediate stage in the mechanism of ovulation. f2 prostaglandin, f2 alpha, on the other hand, represents in numerous animals, but not in wome ...	1975	175448
[diagnosis of human active or latent human brucellosis by lymphocyte transformation. inhibition of leukocyte migration and passive hemagglutination. possible interference between brucellosis and some mental disorders].	passive hemagglutination tests (h.t.), involving the coating of a soluble b. abortus antigen onto sheep red blood cells through chromium chloride, were always negative in non-brucellic subjects. positive h.t., even at low titers (1:50), were associated with positive specific lymphoblastic (t.t.l.) and inhibition of leucocyte migration (i.m.l.) tests in 13 patients. these 3 tests were negative in 17 control brucellosis-free individuals. positive h.t. confirmed a clinical diagnosis of brucellosis ...	1975	175477
[combined vaccination of sheep against anthrax, pox and anaerobic infections].		1975	175545
methaemoglobin reduction and nadh-dependent methaemoglobin reductase activity following dnbp-and nitrite induced methaemoglobinemia in sheep.		1976	175641
the possible viral etiology of multiple sclerosis.		1975	175649
effects of fasting and theophylline on alanine-stimulated glucagon secretion in neonatal and infant sheep.	plasma concentrations of glucose, alanine, and glucagon were measured after 24 hour fasting in newborn and infant sheep and in response to infusion of alanine alone and concurrently with theophylline. the plasma glucagon response to alanine was minimal in newborn sheep; in contrast, alanine produced a brisk response in plasma glucagon concentration in infant sheep. glucose concentrations were unchanged in both groups. theophylline enhanced blood glucagon and glucose responses to alanine in newbo ...	1976	175657
immunochemical studies with a specific antiserum to rabbit fibroblast collagenase.	1. antisera were raised against the collagenase from rabbit synovial fibroblasts and characterized by immunoprecipitation and immunoinhibition reactions. 2. immunoglobulins from the antisera were potent inhibitors of the action of rabbit collagenase on both reconstituted collagen fibrils and collagen in solution. 3. the antibody-binding fragment, fab', produced by digesting the igg (immunoglobulin g) with pepsin, inhibited collagenase activity just as well as whole igg. 4. a specific antiserum t ...	1975	175786
studies on fesv induced sarcomata in sheep with particular reference to the regional lymphatic system.	inocula of cultured sheep cells that had been transformed with fesv were injected into the legs of sheep so that the changes in the cellular and humoral composition of the efferent lymph from the regional node could be studied throughout the immune responses. the times at which immunoblasts and specific antibodies appeared in the lymph were similar to those recorded during responses to conventional antigens. the antiboides were mainly 7s, g1 immunoglobulins directed against virion antigens on th ...	1975	175813
cellular localization of cyclic amp in periodontal tissues during experimental tooth movement in cats.	using an immune-histochemical method, cyclic amp was localized in cells of periodontal tissues in orthodontically-treated cats. sixteen cats were treated for periods ranging from 1 hour to 4 weeks. fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic amp antibodies, followed by sequential incubations with sheep anti-rabbit igg, rabbit anti-peroxidase igg and horseradish peroxidase. in the final step, the peroxidase was demonstrated by the diaminobenzid ...	1976	175896
chemical carcinogenesis in the nervous system.		1976	176123
experiments in border disease. viii. propagation and properties of a cytopathic virus.		1976	176184
measurement of apolipoprotein a-i in rat high density lipoprotein and in rat plasma by radioimmunoassay.	a double antibody radioimmunoassay (ria) for rat apolipoprotein a-i is reported. the apoa-i isolated from delipidated hdl by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (sds), and its amino acid composition resembled that reported by others. apoa-i was iodinated by lactoperoxidase and the resulting 125i-apoa-i was purified by gel filtration. up to 93% of 125i-apoa-i was precipitable by antibody and greater than 99% of bound 125i-apoa-i was ...	1976	176289
proceedings: heterogeneity of adrenocorticotrophin and its secretion by pituitary cells.		1976	176300
cyclic amp in sheep ovarian follicles: site of production and response to gonadotrophins.	this study was undertaken (i) to establish a relationship between cyclic amp (camp) production and the degree of lh and fsh stimulation; (ii) to determine the effects of various gonadotrophins on follicular formation of camp; and (iii) to identify the precise intrafollicular site of camp formation. the formation of camp increased rapidly in follicles exposed to lh. maximum concentrations were reached after 90 min and were maintained for 180 min. extracellular release of camp increased steadily t ...	1976	176356
lack of infectivity of bovine leukemia (c-type) virus to rats.	sprague-dawley rats were given ip injections of bovine culture and sheep cultures of bovine leukemia virus (blv) and gross passage-a leukemia virus [mulv(g)]. sera were tested to blv antigens. blv did not induce tumors in sprague-dawley rats, but the rats were susceptible to mulv(g) at low doses.	1976	176402
initial studies on "six months disease" in sheep.	enterotoxaemia in sheep due to clostridium welchii type d was indicated by field and laboratory investigations in nepal. morphological, cultural, biochemical, biological and toxin-producing characteristics observed were used to type the isolates. in anaerobic meat medium, all isolates produced pinkish discoloration of meat. all the strains fermented lactose, maltose, dextrose and sucrose whereas, salicine was fermented only by 17 strains. all but five strains were mr negative. out of 200 isolate ...	1976	176766
isolation of louping-ill virus from a roe deer (capreolus capreolus).		1976	176767
infectivity of visna virus dna.		1976	176812
proceedings: species variation in motor transmission to the retractor penis muscle.		1976	177140
immunodeficiency in the chicken. iv. an immunological study of infectious bursal disease.	chickens inoculated orally with infectious bursal disease virus (ibdv) 1 day after hatching subsequently showed a 50% incidence of immunodeficiency but little mortality. antibody responses against ibdv and to immunization with sheep red blood cells (srbc) or human serum albumin (hsa) were suppressed. serum igg concentration was decreased while igm occurred exclusively in its 7s monomeric form (migm). an allotypic marker of chicken igm (mla) was lacking in migm derived from ibdv-infected birds. t ...	1976	177236
[epidemiology of human and animal brucellosis in western africa. the results of six studies in the ivory coast, upper volta, and nigeria].	brucellosis has a two-fold importance in public health: the transmission of the disease to man by contact with animals or ingestion of raw milk is of direct importance; of indirect importance is the loss of animal protein. the authors have carried out ten epidemiological investigations in different bio-climatic zones of west africa. more than 120 villages were evaluated. in each village, 100 persons were chosen at random and all or part of the animal population was also studied. three tests were ...	1976	177324
epstein-barr virus interactions with human lymphocyte subpopulations: virus adsorption, kinetics of expression of epstein-barr virus-associated nuclear antigen, and lymphocyte transformation.	in order to further understand epstein-barr virus (ebv)-lymphocyte interactions, we investigated a chain of events including: (i) ebv binding to human lymphocyte subpopulations; (ii) the earliest appearance of ebv-determined nuclear antigen (ebna) in the lymphocytes after ebv infection; and (iii) establishment of continuous lymphoblastoid cell lines (lcl) by infecting with ebv different types of lymphocyte preparations from the same as well as from different donors. by using direct membrane immu ...	1976	177360
exposure of thyroid slices to thyroid-stimulating hormone induces refractoriness of the cyclic amp system to subsequent hormone stimulation.	these studies evaluated the influence of an initial exposure of thyroid slices to thyroid-stimulating hormone (tsh) on the subsequent responsiveness to the hormone. bovine thyroid slices were incubated with or without 50 mu/ml tsh for varying periods and then incubated in hormone-free medium for varying periods. subsequently, slices were incubated for 20 min with 10 mm theophylline and with or without tsh. cylic amp was measured after the third incubation. phosphodiesterase and adenylate cylase ...	1976	177452
characteristics of a virus causing a pox disease in sheep and goats in kenya, with observation on the epidemiology and control.	the sheep and goat pox viruses isolated in kenya are not host specific as in the middle east and india. the virus strains from one species are of a similar pathogenicity for the other, and the same virus appears to occur in the field outbreaks in mixed flocks. a silent infection with no skin lesions occurs in the field outbreaks. the virus grows well in lamb testis cell cultures, it does not haemadsorb nor haemagglutinate and is ether sensitive. most strains could not readily be adapted to grow ...	1976	177699
biology of prolactin.		1975	178000
the chemistry of the adrenocorticotropins and the melanotropins.		1975	178001
structure-activity relationships of adrenocorticotropin.		1975	178002
immunochemistry of adrenocorticotropin, melanotropins and lipotropins.		1975	178003
early diagnosis of virus-caused vesicular rashes by immunofluorescence on skin biopsies. i. varicella, zoster and herpes simplex.	the use of immunofluorescence (if) for the rapid identification of varicella-zoster (v-z) and herpes simplex (hs) antigen in frozen sections of biopsies of early non-vesicular skin lesions was investigated. direct if, using fluorescein- isothiocyanate- conjugated immuoglobulin (fitc ig) of paired human anti-v-z sera and fitc-conjugated negative and positive anti-v-z-monkey ig, yielded specific fluorescence of virus antigen in all 14 varicella cases investigated and in 10/11 zoster cases. in cont ...	1976	178050
malignant hypertension with hypokalemia in a patient with renin-producing pulmonary carcinoma.		1975	178085
effect of delayed evisceration on the microbial quality of meat.	the postomortem invasion of muscle and other tissues by bacteria from the intestinal tract was studied with the use of radioactive tracers. the injection of 14c-labeled bacteria or spores into the intestines of guinea pig carcasses within 24 h of death resulted in the rapid spread of 14c throughout carcasses. when live bacteria were injected along with the labeled cells, it was not possible to isolate viable organisms from the body tissues if the living animal had been exposed to the bacteria. i ...	1976	178276
experimental infection of monkeys with viruses of the tick-borne encephalitis complex: degenerative cerebellar lesions following inapparent forms of the disease or recovery from clinical encephalitis.	rhesus, patas and vervet monkeys were infected i.c. or i.n. with three viruses of the tick-borne encephalitis complex (tbe) as follows: turkish tick-borne encephalitis virus (tte), louping-ill virus and central european tick-borne encephalitis virus (cete). the incidence of overt clinical signs of disease varied according to the virus that was used for the inoculations. tte proved to be more pathogenic for monkeys than the other two members of the complex, whilst cete was the least pathogenic. i ...	1976	178337
modified hemolytic plaque technique for the detection of bluetongue virus antibody-forming cells.	a hemolytic plaque assay was developed for the detection of antibody-forming cells to bluetongue virus (btv). sheep erythrocytes (srbc), onto which btv had been absorbed, served as the indicator of lysis due to the presence of btv antibody-forming cells. the ratio of btv to srbc was found to be critical for optimum hemolytic plaque formation. for routine use, 50 mul of 12% btv srbc, 0.1 ml of a spleen cell suspension, and 0.5 ml of 0.5% agarose in a balanced salt solution were mixed and plated o ...	1976	178602
effect of pregnancy and lactation on triglycerides of very-low-density lipoproteins of rat plasma.	triglyceride in the blood of rats increased during pregnancy, decreased to control during lactation, and increased again on weaning. the triglyceride content of the very-low-density lipoproteins (d less than 1.006 g/ml) changed in parallel with that of the plasma, and its magnitude indicated that it was chiefly responsible for the transport of triglycerides in the blood. these changes were accompanied by changes in the electrophoretic pattern of the lipoproteins of rat plasma, but no such change ...	1976	178703
human lymphocyte subpopulations: giant srbc rosettes.	human thymus-derived lymphocytes have the ability to form rosettes with sheep red blood cells (srbc) in vitro. in the investigation of rosettes of peripheral blood lymphocytes of 10 normal subjects, the number of srbc adhering to the lymphocyte in each of 100 rosettes was assessed. the percentage of rosettes with srbc greater than or equal to 36 per rosette was only 1.2 +/- 0.5. these were defined as giant srbc rosettes. peripheral blood lymphocytes were stimulated in vitro by four mitogens: sod ...	1976	178802
fetal metabolism and fetal growth.		1976	178867
investigations on adenosine 3',5'-monophosphate phosphodiesterase in ram semen and initial characterization of a sperm-specific isoenzyme.	phosphodiesterase is shown to occur in ram semen, and its activity to be higher in spermatozoa than in seminal plasma. using similar substrate levels, the rate at which adenosine 3',5'-monophosphate (cyclic amp) is metabolized by phosphodiesterase in spermatozoa is about 100 times higher than that of cyclic amp synthesis by adenylate cyclase. in spermatozoa, phosphodiesterase is present partly in a soluble form, and partly bound; both forms can be extracted by sonication. the soluble enzyme (ph ...	1976	178869