Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model. | rainbow trout, oncorhynchus mykiss (walbaum), are able to raise a protective immune response against aeromonas salmonicida subsp. salmonicida (as) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under danish mariculture conditions. we elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. rainbow trout juveniles were vaccinated by intra ... | 2017 | 27593619 |
| new species of plagioporus stafford, 1904 (digenea: opecoelidae) from california, with an amendment of the genus and a phylogeny of freshwater plagioporines of the holarctic. | plagioporus hageli n. sp. is described from the intestine of oncorhynchus mykiss (walbaum) collected from the river yuba, california, usa. of the accepted, nominal species of plagioporus stafford, 1904 from the nearctic, the new species is morphologically similar to three intestinal species from the western usa parasitising diadromous fishes, including plagioporus shawi (mcintosh, 1939), plagioporus kolipinskii tracey, choudhury, cheng & ghosh, 2009 and plagioporus siliculus sinitsin, 1931, and ... | 2016 | 27638729 |
| protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss). | the purpose of the study was to analyze seminal plasma composition, sperm production, and sperm motility over the course of the spawning season in the common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss). the highest mean percent of motile sperm (carp, 98.3 ± 1.6%; rainbow trout, 92.8 ± 5.6%) and highest spermatozoon velocity (carp, 286.3 ± 7.8 μm sec(-1) ; rainbow trout, 245.3 ± 8.3 μm sec(-1) ) were observed in the middle phase of the spawning period, at 5 sec post-activation. ... | 2016 | 27641056 |
| effects of dietary yeast inclusion and acute stress on post-prandial whole blood profiles of dorsal aorta-cannulated rainbow trout. | yeast is a potential alternative to fish meal in diets for farmed fish, yet replacing more than 50 % of fish meal results in reduced fish growth. in a 4-week experiment, 15 rainbow trout (oncorhynchus mykiss) were cannulated and fed three diets each week: 30 % fish meal as a control (fm); 60 % replacement of fish meal protein, on a digestible basis, with saccharomyces cerevisiae (sc); and 60 % replacement with wickerhamomyces anomalus and s. cerevisiae mix (wa). blood was collected at 0, 3, 6, 1 ... | 2017 | 27677483 |
| recombinant scfv antibodies against infectious pancreatic necrosis virus isolated by flow cytometry. | infectious pancreatic necrosis is a significant disease of farmed salmonids in china. in this study, a single chain variable fragment (scfv) antibody library derived from rainbow trout (oncorhynchus mykiss) and viral protein vp2 of a chinese infectious pancreatic necrosis virus (ipnv) isolate chrtm213 were co-expressed by a bacterial display technology. the library was subjected to three rounds of screening by flow cytometry (fcm) to select ipnv specific antibodies. six antibody clones with diff ... | 2016 | 27678027 |
| detection of infectious pancreatic necrosis virus from the leeches hemiclepsis marginata and hirudo medicinalis. | leeches have been reported to harbor several important fish pathogens, including spring viremia of carp virus, infectious hematopoietic necrosis virus (ihnv), and viral hemorrhagic septicemia virus (vhsv), and also may contain blood protozoa. in the present study, leeches were collected from water bodies located in kurdistan province, iran. the specimens were tested for ihnv, vhsv, and infectious pancreatic necrosis virus (ipnv) using the pcr method. the results showed that two different species ... | 2016 | 27687675 |
| effect of feed supplementation with kynurenic acid on the morphology of the liver, kidney and gills in rainbow trout (oncorhynchus mykiss walbaum, 1792), healthy and experimentally infected with yersinia ruckeri. | kynurenic acid (kyna) is an endogenous substance produced on the kynurenine pathway which is primarily known for its neuroactive properties. recently, it has been proven that kyna is a selective ligand for g protein-coupled receptor (gpr 35), presented on immunocompetent cells such as t lymphocytes. this opens up new possibilities of its application as an immunostimulating substance in aquaculture. thus far, no histopathological investigations in fish have been completed to evaluate influence of ... | 2016 | 27690267 |
| proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (oncorhynchus mykiss). | flavobacterium psychrophilum (fp) is the causative agent of bacterial cold water disease (bcwd) which causes appreciable economic losses in rainbow trout aquaculture. we previously reported development of a genetic line, designated ars-fp-r that exhibits higher survival relative to a susceptible line, designated ars-fp-s, following either laboratory or natural on-farm challenge. the objectives of this study were to determine the temporal kinetics of gene expression between experimentally-challen ... | 2016 | 27693200 |
| piscirickettsia salmonis imbalances the innate immune response to succeed in a productive infection in a salmonid cell line model. | piscirickettsia salmonis is a facultative intracellular bacterium that causes the disease called "salmon rickettsial syndrome". attempts to control this disease have been unsuccessful, because existing vaccines have not achieved the expected effectiveness and the antibiotics used fail to completely eradicate the pathogen. this is in part the product of lack of scientific information that still lacks on the mechanisms used by this bacterium to overcome infected-cell responses and survive to induc ... | 2016 | 27723816 |
| occurrence of intersex in wild freshwater fish in slovenian rivers: a histological evaluation. | the aim of this preliminary research was to establish if there are intersex occurrences in wild freshwater fish in slovenian rivers and streams. in the first study we evaluated all fish species of both sexes obtained from the river ljubljanica from its source to mouth. in the second study we focused on the rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta m. fario) males from 30 rivers and streams in different parts of slovenia. the male gonads were histologically assessed for th ... | 2016 | 27749265 |
| acute toxicity of peroxy sulfonated oleic acids (psoa) to freshwater aquatic species and sludge microflora as observed in laboratory environments. | peroxy sulfonated oleic acids (psoa) is a novel surfactant peracid. the commercial applications of psoa result in the chemical primarily being disposed of via industrial waste water effluent. given this manner of disposal, it is important to understand the aquatic hazards of the chemical to better assess the risk posed to aqueous environments. acute aquatic toxicity laboratory experiments were performed to evaluate aquatic hazards and were conducted according to standard oecd test guidelines wit ... | 2015 | 27752422 |
| phylogeny of the viral hemorrhagic septicemia virus in european aquaculture. | one of the most valuable aquaculture fish in europe is the rainbow trout, oncorhynchus mykiss, but the profitability of trout production is threatened by a highly lethal infectious disease, viral hemorrhagic septicemia (vhs), caused by the vhs virus (vhsv). for the past few decades, the subgenogroup ia of vhsv has been the main cause of vhs outbreaks in european freshwater-farmed rainbow trout. little is currently known, however, about the phylogenetic radiation of this ia lineage into subordina ... | 2016 | 27760205 |
| consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (oncorhynchus mykiss). | rainbow trout (oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (emb) and deltamethrin (dm) during outbreaks of caligus rogercresseyi in a farm located in southern chile (hornopiren, chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome p450 1a (cyp1a), flavin-containing monooxygenase (fmo) and glutathione s-transferase (gst) in different tissues. consecutive and alternating ... | 2017 | 27765649 |
| corrigendum to "in vivo virulence of viral haemorrhagic septicaemia virus (vhsv) in rainbow trout oncorhynchus mykiss correlates inversely with in vitro mx gene expression" [vet. microbiol. 187 (2016) 31-40]. | 2016 | 27771070 | |
| replication and shedding kinetics of infectious hematopoietic necrosis virus in juvenile rainbow trout. | viral replication and shedding are key components of transmission and fitness, the kinetics of which are heavily dependent on virus, host, and environmental factors. to date, no studies have quantified the shedding kinetics of infectious hematopoietic necrosis virus (ihnv) in rainbow trout (oncorhynchus mykiss), or how they are associated with replication, making it difficult to ascertain the transmission dynamics of this pathogen of high agricultural and conservation importance. here, the repli ... | 2017 | 27771253 |
| increasing virulence, but not infectivity, associated with serially emergent virus strains of a fish rhabdovirus. | surveillance and genetic typing of field isolates of a fish rhabdovirus, infectious hematopoietic necrosis virus (ihnv), has identified four dominant viral genotypes that were involved in serial viral emergence and displacement events in steelhead trout (oncorhynchus mykiss) in western north america. to investigate drivers of these landscape-scale events, ihnv isolates designated 007, 111, 110, and 139, representing the four relevant genotypes, were compared for virulence and infectivity in cont ... | 2016 | 27774291 |
| ecotoxicity interspecies qaar models from daphnia toxicity of pharmaceuticals and personal care products. | pharmaceutical and personal care products (ppcps) became a class of contaminants of emerging concern because are ubiquitously detected in surface water and soil, where they can affect wildlife. ecotoxicological data are only available for a few ppcps, thus modelling approaches are essential tools to maximize the information contained in the existing data. in silico methods may be helpful in filling data gaps for the toxicity of ppcps towards various ecological indicator organisms. the good corre ... | 2016 | 27775436 |
| st2 from rainbow trout quenches tlr signalling, localises at the nuclear membrane and allows the nuclear translocation of myd88. | the mammalian interleukin 1 receptor-like 1 receptor (il1rl1), commonly known as st2, is thought to downregulate tlr signalling by sequestering the signalling adapter myd88 (myeloid differentiation primary response protein 88). st2 sequences are known in several fish species, but none of them have functionally been examined. we characterised st2 from rainbow trout (oncorhynchus mykiss) and the structure of its encoding gene. the primary sequence of st2 is only weakly conserved from fish to human ... | 2017 | 27776995 |
| ecological risk assessment of pharmaceuticals in the receiving environment of pharmaceutical wastewater in pakistan. | the pharmaceutical industry of pakistan is growing with an annual growth rate of 10%. besides this growth, this industry is not complying with environmental standards, and discharging its effluent into domestic wastewater network. only limited information is available about the occurrence of pharmaceutical compounds (pcs) in the environmental matrices of pakistan that has motivated us to aim at the occurrence and ecological risk assessment of 11 pcs of different therapeutic classes in the wastew ... | 2017 | 27810578 |
| proteomic analysis of muscle tissue from rainbow trout (oncorhynchus mykiss) fed dietary β-glucan. | the aim of this study was to examine the changes in muscle proteome of the rainbow trout fed dietary β-glucan. the experimental diets contained 0 (control), 0.1% and 0.2% β-1,3/1,6 yeast glucan. first, feeding larvae were fed to apparent satiation nine times per day with their respective diets over two months. the percentage of body weight gain and feed efficiency of fish fed 0.2% diet was significantly higher than those of fish fed the control and 0.1% diets. fish fed the control and 0.2% diets ... | 2016 | 27822248 |
| multiple gene and transcript variants encoding trout c-polysaccharide binding proteins are differentially but strongly induced after infection with aeromonas salmonicida. | two 'trout c-polysaccharide-binding proteins,' tcbp1 and -2, with relevance to early inflammatory events have been discovered in the last century. the present study characterises the respective cdna sequences from rainbow trout (oncorhynchus mykiss), including multiple tcbp1 transcript variants. these variants are generated either by the use of alternative splice sites or the exclusion of exons. the longest mrna isoform, tcbp1-1, encodes a 245-aa protein with a large signal peptide and a complem ... | 2017 | 27836722 |
| complete genomic sequence of an infectious pancreatic necrosis virus isolated from rainbow trout (oncorhynchus mykiss) in china. | infectious pancreatic necrosis (ipn) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in china. however, no gene sequence of any chinese infectious pancreatic necrosis virus (ipnv) isolates was available. in the study, moribund rainbow trout fry samples were collected during an outbreak of ipn in yunnan province of southwest china in 2013. an ipnv was isolated and tentatively named chrtm213. we determined the full genome sequence of the ipnv ... | 2017 | 27858313 |
| characterisation of zbtb46 and dc-script/znf366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish. | zbtb46 and dc-script/znf366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. in this study, the zbtb46 and dc-script/znf366 homologues were identified in rainbow trout oncorhynchus mykiss and their expression analysed in vivo and in vitro. as transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. their expression was differentially modulated by bacterial and viral pamps in ... | 2016 | 27889532 |
| genome sequence of the fish pathogen yersinia ruckeri strain 150, isolated from diseased rainbow trout. | we present here the draft genome of a pathogenic yersinia ruckeri strain, isolated from rainbow trout (oncorhynchus mykiss) affected by enteric redmouth disease. the chromosome has 3,826,775 bp, a gc content of 46.88%, and is predicted to contain 3,538 coding sequences. the data will be useful for comparative pathogenicity studies. | 2016 | 27908991 |
| a dna vaccine encoding the viral hemorrhagic septicemia virus genotype ivb glycoprotein confers protection in muskellunge (esox masquinongy), rainbow trout (oncorhynchus mykiss), brown trout (salmo trutta), and lake trout (salvelinus namaycush). | the viral hemorrhagic septicemia virus (vhsv) is one of the most serious fish pathogens. in 2003, a novel sublineage (genotype ivb) of this deadly virus emerged in the great lakes basin causing serious fish kills. we have previously demonstrated that a dna plasmid (pcdna), containing a cytomegalovirus (cmv) promoter and the viral hemorrhagic septicemia virus (vhsv) genotype ivb glycoprotein (g) gene insert (designated pvhsivb-g) confers moderate protection in muskellunge (esox masquinongy), a hi ... | 2016 | 27912771 |
| aeromonas salmonicida type iii secretion system-effectors-mediated immune suppression in rainbow trout (oncorhynchus mykiss). | aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. together with other pathogens, it is characterized by the presence of a type 3 secretion system (t3ss). the t3ss is the main virulence mechanism of a. salmonicida. it is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributin ... | 2017 | 27923746 |
| symbiont-derived sphingolipids modulate mucosal homeostasis and b cells in teleost fish. | symbiotic bacteria and mucosal immunoglobulins have co-evolved for millions of years in vertebrate animals. symbiotic bacteria products are known to modulate different aspects of the host immune system. we recently reported that flectobacillus major is a predominant species that lives in the gill and skin mucosal surfaces of rainbow trout (oncorhynchus mykiss). f. major is known to produce sphingolipids of a unique molecular structure. here we propose a role for f. major and its sphingolipids in ... | 2016 | 27966609 |
| the flagellar master operon flhdc is a pleiotropic regulator involved in motility and virulence of the fish pathogen yersinia ruckeri. | to investigate the function of the master flagellar operon flhdc in the fish pathogen yersinia ruckeri and compare the effect of a constructed flhd mutation to a naturally occurring flir mutation causing loss-of-motility in emergent biotype 2 (bt2) strains. | 2017 | 27981729 |
| characterisation of arginase paralogues in salmonids and their modulation by immune stimulation/ infection. | in this study we show that four arginase isoforms (arg1a, arg1b, arg2a, arg2b) exist in rainbow trout (oncorhynchus mykiss) and atlantic salmon (salmo salar). we have characterised these molecules in terms of a) sequence analysis, b) constitutive expression in different tissues, and modulated expression following c) stimulation of head kidney macrophages in vitro, or d) vaccination/infection with yersinia ruckeri and e) parasite infection (agd caused by paramoeba perurans and pkd caused by tetra ... | 2017 | 28025160 |
| effect of ammonia-n and pathogen challenge on complement component 8α and 8β expression in the darkbarbel catfish pelteobagrus vachellii. | the complement components c8α and c8β mediate the formation of the membrane attack complex (mac) to resist pathogenic bacteria and play important roles in innate immunity. full-length complement c8α (pv-c8α) and c8β (pv-c8β) cdna were identified in the darkbarbel catfish pelteobagrus vachellii, and their mrna expression levels were analyzed after ammonia-n and pathogen treatment. the pv-c8α gene contained 1983 bp, including a 1794-bp open reading frame (orf) encoding 598 amino acids. the pv-c8β ... | 2017 | 28027983 |
| characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4. | the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ... | 2017 | 28074223 |
| expansion and differentiation of igm(+) b cells in the rainbow trout peritoneal cavity in response to different antigens. | to date, intraperitoneal (i.p.) injection seems to be the most effective vaccination route in aquaculture, as many i.p. administered fish vaccines are capable of conferring strong and long-lasting immune responses. despite this, how peritoneal leukocytes are regulated upon antigen encounter has only been scarcely studied in fish. although, in the past, myeloid cells were thought to be the main responders to peritoneal inflammation, a recent study revealed that igm(+) b cells are one of the main ... | 2017 | 28088353 |
| molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (oncorhynchus mykiss). | a novel virus, rainbow trout orthomyxovirus (rbtov), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (oncorhynchus mykiss) in idaho, usa. the virus grew optimally in the chse-214 cell line at 15°c producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. electron microscopy of preparations from infected cell cultures revealed the presence of typical orthomyxovirus particles. the ... | 2017 | 28088362 |
| gyrodactylus salmonis infection impairs the olfactory system of rainbow trout. | monogenean worms are ectoparasites that are known to be infectious to a wide variety of fish. few species of monogenean parasites have been reported in the olfactory chamber of fish in current peer-reviewed literature. however, the impacts of these parasites on the olfactory system are not well understood. in this study, the effects of gyrodactylus salmonis on the olfactory system structure and performance were investigated in rainbow trout (oncorhynchus mykiss). the olfactory performance of the ... | 2017 | 28105714 |
| molecular characterization of infectious pancreatic necrosis virus strains isolated from the three types of salmonids farmed in chile. | the infectious pancreatic necrosis virus (ipnv) causes significant economic losses in chilean salmon farming. for effective sanitary management, the ipnv strains present in chile need to be fully studied, characterized, and constantly updated at the molecular level. | 2017 | 28143585 |
| rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens. | commensal bacteria co-exist on the mucosal surfaces of all vertebrates. the host's mucosal immune system must tolerate commensals while fighting pathogens. one of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pigr). rainbow trout pigr is known to transport igt and igm across epithelia. however, other biological functions for trout pigr or t ... | 2017 | 28150752 |
| lhx8 interacts with a novel germ cell-specific nuclear factor containing an nbl1 domain in rainbow trout (oncorhynchus mykiss). | lhx8 is an important transcription factor that is preferentially expressed in germ cells. lhx8 null mice are infertile due to lack of oocytes and impairment of the transition from primordial follicles to primary follicles. lhx8 deficiency also affects the expression of many important oocyte-specific genes. in this study, we report the characterization of rainbow trout lhx8 genes and identification of a novel germ cell-specific nuclear factor that interacts with lhx8. two lhx8 genes, lhx8a and lh ... | 2017 | 28151980 |
| subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ... | 2017 | 28182704 |
| in vivo adherence of flavobacterium psychrophilum to mucosal external surfaces of rainbow trout (oncorhynchus mykiss) fry. | the adherence of flavobacterium psychrophilum to surfaces of epithelial tissues has been inconclusively suggested as a mechanism, which enables the bacterium to invade the host. hence, the present study aimed to examine the adherence of the cells of two colony phenotypes, smooth and rough, of f. psychrophilum to mucosal tissues of rainbow trout fry and to test the skin mucus as a nutrient for the growth of f. psychrophilum. fish were immersed in water containing 10(6 ) cfu( ) ml(-1) f. psychroph ... | 2017 | 28188658 |
| pathogenicity of mexican isolates of aeromonas sp. in immersion experimentally-infected rainbow trout (oncorhynchus mykiss, walbaum 1792). | ten species of aeromonas have been previously identified in farmed rainbow trout from mexico. the aim of the current study was to investigate the pathogenicity of 10 aeromonas isolates belonging to 10 different aeromonas species in immersion experimentally-infected rainbow trout fry. isolates of a. bestiarum, a. hydrophila, a. salmonicida, and a. veronii produced significant mortality (8.8%, 12.2%, 18.8%, and 8.8%, respectively). isolates of a. caviae and a. sobria produced no significantly mort ... | 2017 | 28209550 |
| application of passive sampling to characterise the fish exometabolome. | the endogenous metabolites excreted by organisms into their surrounding environment, termed the exometabolome, are important for many processes including chemical communication. in fish biology, such metabolites are also known to be informative markers of physiological status. while metabolomics is increasingly used to investigate the endogenous biochemistry of organisms, no non-targeted studies of the metabolic complexity of fish exometabolomes have been reported to date. in environmental chemi ... | 2017 | 28216558 |
| dna vaccination partially protects muskellunge against viral hemorrhagic septicemia virus (vhsv-ivb). | a dna vaccine containing the glycoprotein (g) gene of the north american viral hemorrhagic septicemia virus (vhsv) genotype ivb was developed to evaluate the immune response of fish following vaccination and evaluate its efficacy in protecting a susceptible species, the muskellunge esox masquinongy, against vhsv-ivb challenge. seven weeks (539 degree-days) following vaccination with 10 μg of either pvhsivb-g or a control plasmid, muskellunge were challenged by immersion with 10(5) plaque-forming ... | 2017 | 28225652 |
| outbreak of infectious pancreatic necrosis virus (ipnv) in farmed rainbow trout in china. | infectious pancreatic necrosis virus (ipnv) is a member of the aquabirnavirus genus, which caused mass mortality (nearly 100%) in farmed rainbow trout (oncorhynchus mykiss) in aquaculture farms in 2016, china. major clinical signs included decreased appetite, mucous-like stools, and darkened pigmentation. pathological changes in moribund fish were observed, such as marked vacuolar degeneration of the pancreatic cells with pyknotic nucleus and decreased zymogen granules, severe hemorrhage in the ... | 2017 | 28232067 |
| dietary β-glucans differentially modulate immune and stress-related gene expression in lymphoid organs from healthy and aeromonas hydrophila-infected rainbow trout (oncorhynchus mykiss). | although β-glucans stimulating effects have already been demonstrated on the immune system of numerous animal species, available data remain relatively variable and more research should be done regarding the complexity of underlying mechanisms. in this context, the present study aimed to evaluate the stress and immune-related effects of dietary β-glucans (i.e. macrogard(®)) by considering a number of influencing factors such as the dose (0, 0.1, 0.2 and 0.5% in food), feeding duration (15 versus ... | 2017 | 28232282 |
| bulgarian marine and freshwater fishes as a source of fat-soluble vitamins for a healthy human diet. | the aim of the present study evaluates the fat-soluble vitamins all-trans retinol (vitamin a), cholecalciferol (vitamin d₃) and α-tocopherol (vitamin e) content in the fresh edible tissue of bulgarian fish species: marine-grey mullet (mugil cephalus) and bonito (sarda sarda), and freshwater-rainbow trout (oncorhynchus mykiss) and common carp (cyprinus carpio). the sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction with n-hexane. all-trans retinol, ... | 2013 | 28239118 |
| dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (oncorhynchus mykiss) measured using a novel gexp multiplex, rt-pcr assay. | a gexp multiplex, rt-pcr assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. the dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a ... | 2017 | 28239633 |
| microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and saccharomyces cerevisiae) in juvenile rainbow trout (oncorhynchus mykiss). | this study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% saccharomyces cerevisiae respectively) or the mixture of both. | 2017 | 28256031 |
| genetic and serological diversity of flavobacterium psychrophilum isolates from salmonids in united kingdom. | flavobacterium psychrophilum is one of the most important bacterial pathogens affecting cultured rainbow trout (oncorhynchus mykiss) and is increasingly causing problems in atlantic salmon (salmo salar l.) hatcheries. little is known about the heterogeneity of f. psychrophilum isolates on uk salmonid farms. a total of 315 f. psychrophilum isolates, 293 of which were collected from 27 sites within the uk, were characterised using four genotyping methods and a serotyping scheme. a high strain dive ... | 2017 | 28284613 |
| preliminary study of an oral vaccine against infectious hematopoietic necrosis virus using improved yeast surface display technology. | infectious hematopoietic necrosis virus (ihnv) is a common pathogen that causes severe disease in the salmonid aquaculture industry. because oral vaccines induce more efficient mucosal immunity than parenteral immunization, an oral vaccine was developed with an improved yeast cell surface display technology to induce an immune response to ihnv. the oral yeast vaccine, designated eby100/pyd1-bi-g, was delivered orally to rainbow trout (oncorhynchus mykiss) on days 1 and 32, and the nonspecific an ... | 2017 | 28285182 |
| effect of dietary β-glucan on growth, survival and regulation of immune processes in rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida. | the present study evaluated the effects of dietary β-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. thereafter, rainbow trout (oncorhynchus mykiss) were infected with aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. in general, higher dietary β-glucan levels obviously improved specific growth rate (sgr), weight gain (wg) and feed efficiency (fe) (p ≤ 0.05). survival rates in β-glu ... | 2017 | 28286256 |
| hypothermic storage of isolated spermatogonia and oogonia from rainbow trout (oncorhynchus mykiss). | a growing number of fish species are endangered due to human activities. a short- or long-time preservation of gametes could conserve genetic resources of threatened fish species. the aim of this study was to evaluate a hypothermic condition for short-term preservation of spermatogonia and oogonia cells isolated from immature transgenic rainbow trout, oncorhynchus mykiss, and to determine the maximum time point for further transplantation. viability rate of germ cells was investigated after isol ... | 2017 | 28302378 |
| comparison of selected nonlethal samples from adult steelhead for detection of infectious hematopoietic necrosis virus using cell culture. | nonlethal sampling techniques have previously been evaluated for detection of a variety of viral salmonid pathogens. however, many of these studies have used molecular assays in lieu of widely accepted cell culture techniques to evaluate the sampled tissues. samples were collected from female steelhead oncorhynchus mykiss broodstock using three potential nonlethal sampling methods (mucus/skin scrape, pectoral fin clip, and gill tissue biopsy) and evaluated for the presence of infectious hematopo ... | 2017 | 28324676 |
| dietary myrtle (myrtus communis l.) improved non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (oncorhynchus mykiss) fingerlings. | the present study examined the effects of dietary myrtle (myrtus communis l.) on non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (oncorhynchus mykiss) fingerlings. three hundred and sixty fingerlings (6.50 ± 0.55 g (were distributed in twelve cages (65 × 65 × 65 cm) with a metal framework. the study included four treatments repeated in triplicates. the treatments were feeding trouts with experimental diets containing different levels (0, 0.5, 1 and 1.5%) o ... | 2017 | 28330807 |
| antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida. | demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ... | 2017 | 28343146 |
| analytical validation of a reverse transcriptase droplet digital pcr (rt-ddpcr) for quantitative detection of infectious hematopoietic necrosis virus. | infectious hematopoietic necrosis virus (ihnv) is an important pathogen of salmonid fishes. a validated universal reverse transcriptase quantitative pcr (rt-qpcr) assay that can quantify levels of ihnv in fish tissues has been previously reported. in the present study, we adapted the published set of ihnv primers and probe for use in a reverse-transcriptase droplet digital pcr (rt-ddpcr) assay for quantification of the virus in fish tissue samples. the rt-ddpcr and rt-qpcr assays detected 13 phy ... | 2017 | 28347708 |
| different phenotypes of mature biofilm in flavobacterium psychrophilum share a potential for virulence that differs from planktonic state. | flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease and the rainbow trout fry syndrome in salmonid aquaculture worldwide. however, there have been few studies into the capacity of f. psychrophilum to form biofilms and how these cellular accretions differ from planktonic cells or how they affect potential virulence. we evaluated the biofilm formation by three chilean isolates of f. psychrophilum (lm-02-fp, lm-06-fp, and lm-13-fp) and two non-chilean strains (jip02 ... | 2017 | 28361040 |
| invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout. | the literature on cell lines that have been developed from rainbow trout (rt) (oncorhynchus mykiss) is reviewed to illustrate three new terms: invitromatics, invitrome, and invitroomics. invitromatics is defined as the history, development, characterization, engineering, storage, and sharing of cell lines. rt invitromatics differs from invitromatics for humans and other mammals in several ways. nearly all the rt cell lines have developed through spontaneous immortalization. no rt cell line under ... | 2017 | 28374170 |