Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| what's eating you? schistosoma japonicum. | 2004 | 15186042 | |
| [prediction of t-cell epitopes from schistosoma japonicum 28 kda glutathione-s-transferase and identification of their th1 type t-cell epitopes]. | to predict t-cell epitopes of recombinant schistosoma japonicum 28 kda glutathione-s-transferase(gst) with software and identify the th1 type t-cell epitopes by experiments. | 2004 | 15193237 |
| schistosome transcriptome analysis at the cutting edge. | we live in the era of post-genomics, a term that was, until recently, inappropriate when considering the blood flukes of humans because of the relative lack of knowledge of the schistosome genome. the position has, however, changed dramatically following the recent publication of two landmark papers on transcriptome analysis of schistosoma japonicum and schistosoma mansoni. in a quantum leap, both studies report on the identification of many novel genes and genes not previously known from schist ... | 2004 | 15193557 |
| expression and cross-species reactivity of fatty acid-binding protein of clonorchis sinensis. | clonorchis sinensis is a chinese liver fluke that chronically resides in the biliary tract. the fatty acid-binding protein (fabp) is known to play an important role in the intracellular transport of long-chain fatty acids that are obtained by the fluke from the host. although fabp has stimulated considerable interest as a vaccine target candidate, the nature of fabp from c. sinensis (csfabp) remains unclear. in this paper, we describe the cloning and expression of recombinant fabp and immune cro ... | 2004 | 15197581 |
| schistosoma japonicum: establishment of a mouse model that demonstrates concomitant immunity. | 2004 | 15198906 | |
| schistosoma japonicum: isolation and identification of peptides mimicking ferritin epitopes from phage display library. | in an attempt to isolate and identify the antigenic epitopes on ferritin of schistosoma japonicum (sjfer) and to test their protective potentiality against schistosoma japonicum (s.j), polyclonal antisera against sjfer was prepared to screen a 12-mer random peptide library. three rounds of biopanning were performed and resulted in an enrichment. six peptides selected randomly from the third elute were all found to be positive by evaluating the binding to anti-sjfer sera by elisa and western blot ... | 2004 | 15202505 |
| structure and bioactivity of neuropeptide f from the human parasites schistosoma mansoni and schistosoma japonicum. | the blood flukes schistosoma mansoni and schistosoma japonicum inflict immense suffering as agents of human schistosomiasis. previous investigations have found the nervous systems of these worms contain abundant immunoreactivity to antisera targeting invertebrate neuropeptide fs (npfs) as well as structurally similar neuropeptides of the mammalian neuropeptide y (npy) family. here, cdnas encoding npf in these worms were identified, and the mature neuropeptides from the two species differed by on ... | 2004 | 15229227 |
| expression, purification, and human antibody response to a 67 kda vaccine candidate for schistosomiasis japonica. | schistosomiasis remains a leading cause of morbidity and mortality in the developing tropical world, and vaccines to prevent these infections remain a scientific and public health priority. sj67 is a 67 kda schistosoma japonicum surface membrane protein homologous to a family of actin-binding proteins. sj67 is recognized by a mouse monoclonal antibody (mab 6) that confers resistance to challenge infection in passive transfer experiments. these data support sj67 as a potential vaccine candidate f ... | 2004 | 15249044 |
| proceedings of the centenary symposium to celebrate the discovery of schistosoma japonicum. part 2. march 31, 2003. kurume, japan. | 2004 | 15257562 | |
| a comparative study on mouse mhc class i sequences detected in schistosoma japonicum recovered from balb/c (h-2d) and c57bl/6 (h-2b) mice. | the mouse major histocompatibility complex (mhc) class i sequence was detected in all the 8-week-old schistosoma japonicum recovered from balb/c (h-2d) and c57bl/6 (h-2b) mice by in situ polymerase chain reaction (in situ pcr). the signals of the mouse class i mhc sequence were observed in the nuclei of the mesenchymal and reproductive cells of 8-week-old s. japonicum. furthermore, the class i mhc sequence was detected in each dna extracted from s. japonicum cercariae maintained in balb/c and c5 ... | 2004 | 15272738 |
| schistosomiasis control in the people's republic of china. | schistosomes, snail-transmitted trematodes (blood flukes), cause a major parasitic disease that ranks second only to malaria in terms of human suffering in the tropics. schistosoma japonicum has occupied its ecological niche in china for thousands of years; through natural selection it has evolved survival mechanisms that make it difficult (if not impossible) to eradicate. as discussed here by allen ross, li yuesheng, adrian sleigh and don mcmanus, vaccination, in combination with current contro ... | 1997 | 15275103 |
| the pig as a unique host model for schistosoma japonicum infection. | 1996 | 15275220 | |
| [study on the feasibility of schistosoma japonicum infection via oral mucosa]. | 2004 | 15281442 | |
| [expression of proteinase cathepsin l1 gene of schistosoma japonicum in escherichia coli]. | to express the proteinase cathepsin l1 gene of schistosoma japonicum (sjcl1) in escherichia coli jm109 cells. | 2004 | 15281444 |
| [screening of cdna library of schistosoma japonicum with sera from rabbits vaccinated with ultraviolet-attenuated schistosomula]. | to search new potential schistosomiasis vaccine by screening cdna library with sera of rabbits vaccinated with attenuated larvae. | 2004 | 15281445 |
| [detection of biliverdin reductase activity of schistosoma japonicum]. | to explore the presence of biliverdin reductase (br) activity in adult worms of schistosoma japonicum in vitro. | 2004 | 15281457 |
| [studies on the characteristic of interferon-gamma mediating resistance in mice infected with schistosoma japonicum]. | to investigate the molecular characteristic of interferon-gamma mediating protective immunity against schistosomiasis japonica in mice. | 2004 | 15283262 |
| [expression of adenylate kinase of schistosoma japonicum and evaluation on the immunoreactivity of the recombinant protein]. | to express and purify the schistosoma japonicum adenylate kinase (ak) protein of which the cdna sequence was subcloned into the pet32a(+) soluble expression plasmid, and to evaluate its immunoreactivity. | 2004 | 15283267 |
| [schistosoma japonicum in the newly identified areas in hunan province]. | to study the major risk factors and characteristics of schistosoma japonicum in the newly identified areas around the dong ting lake. | 2004 | 15308035 |
| [assessment on the impact of warming climate in winter on schistosomiasis epidemics]. | to assess the potential impact of warming climate in winter to the scale and severity of schistosomiasis epidemics in china. | 2004 | 15308036 |
| [cloning and analysis of the promoter region of schistosoma japonicum 8k cabp gene]. | to clone and analyze the promoter sequence of schistosoma japonicum 8k calcium binding protein(cabp) (sj8cabp) gene. | 2004 | 15321748 |
| invasion by schistosome cercariae: neglected aspects in schistosoma japonicum. | skin invasion by schistosome cercariae was recently discussed in trends in parasitology. however, only schistosoma mansoni was considered, possibly because this species predominates in laboratory studies (at least outside china). one may be tempted to extrapolate from the "model" s. mansoni to other schistosomes, but schistosoma japonicum must not be neglected. this schistosome is distinguishable from others (particularly s. mansoni) by virtue of its remarkable speed and success of migration, as ... | 2004 | 15324727 |
| persistence of the protective immunity to schistosoma japonicum in chinese yellow cattle induced by recombinant 26kda glutathione-s-transferase (resjc26gst). | to observe the long lasting effect of the recombinant sj26gst sub-unit vaccine against schistosoma japonicum in cattle, animals aged from 5 to 12 months were vaccinated with resjc26gst, and were challenged by natural infection 6 months or 12 months after vaccination. worm burdens per cattle and egg burden in tissue (per gram) of cattle with or without vaccination were compared. the results showed that anti-resjc26gst antibodies were produced in vaccinated cattle. following natural infection, the ... | 2004 | 15325043 |
| ultrasound organometry: the importance of body height adjusted normal ranges in assessing liver and spleen parameters among chinese subjects with schistosoma japonicum infection. | hepatosplenic measurements among 550 chinese subjects, aged 3-59 years from yueyang city--a nonendemic area for schistosomiasis in hunan province, china--were performed to define normal ranges of ultrasound organometry for assessing hepatosplenic morbidity in schistosoma japonicum infection. measurements included the size of the liver (left lobe and right lobe), the main portal vein stem, the peripheral periportal vein branches, and spleen length and thickness. the results document the significa ... | 2004 | 15350865 |
| morbidity in schistosomiasis: an update. | schistosomiasis is an important poverty-related health problem and more than 200 million people are infected. this review summarizes papers from april 2003 to june 2004 with a focus on schistosomiasis morbidity and the various factors that affect the level of morbidity in endemic populations. the aim is to provide an update on the current state of knowledge and, hopefully, thereby stimulate continued research interest in this important area. | 2004 | 15353964 |
| expression of selenocysteine-containing glutathione s-transferase in escherichia coli. | evolution of a probable 'glutathione-binding ancestor' resulting in a common thioredoxin-fold for glutathione s-transferases and glutathione peroxidases may possibly suggest that a glutathione s-transferase could be engineered into a selenium-containing glutathione s-transferase (seleno-gst), having glutathione peroxidase (gpx) activity. here, we addressed this question by production of such protein. in order to obtain a recombinant seleno-gst produced in escherichia coli, we introduced a varian ... | 2004 | 15358220 |
| cloning and expression in escherichia coli of a new gene of schistosoma japonicum encoding casein kinase ii beta subunit. | nowadays it is now a focus topic in schistosomiasis research to find ideal vaccine candidates and new drug targets for developing anti-schistosomiasis vaccine. we cloned a new gene, casein kinase ii beta subunit, of schistosoma japonicum (s. japonicum) and express it in escherichia coli (e. coli). | 2004 | 15377422 |
| congenital infection of rabbits with schistosoma japonicum and protective immunity of offspring. | recently congenital infection with schistosoma japonicum (s. japonicum) has been demonstrated in pigs, rabbits, mice and dogs. we explored the rabbit as an animal model for the congenital infection of schistosomiasis japonica and assessed the effect of a congenital s. japonicum infection on the resistance of rabbit kittens to a postnatal challenge infection. | 2004 | 15377423 |
| studies on the emergence of cercariae of schistosoma japonicum from their chinese snail host, oncomelania, hupensis. | 1949 | 15395142 | |
| a preliminary study on the bionomics of oncomelania snails, intermediate hosts of schistosoma japonicum, in kiangsu and chekiang provinces, china. | 1950 | 15425731 | |
| the miniature pig: a unique experimental model for schistosoma japonicum infection. | as part of a search for good animal models for human schistosomiasis, two miniature pigs of the clawn strain (c-1, c-2) were inoculated percutaneously with 200 schistosoma japonicum cercariae of the chinese strain, and the subsequent infection was monitored parasitologically, pathologically and serologically. egg excretion into feces began at 5 weeks post-infection (p.i.) and became pronounced from 8 weeks to 17-20 weeks p.i. the average number of eggs in 1 g feces of each pig at the peak period ... | 2004 | 15464438 |
| gender-associated gene expression in two related strains of schistosoma japonicum. | host inflammatory responses directed against eggs laid by sexually-mature schistosoma japonicum female worms instigate lesion formation and associated clinical pathologies during infection. to identify parasite gene transcripts that associate with egg production and to characterise sexually-mature adult gene expression profiles of two related chinese strains, s. japonicum cdna microarrays were fabricated using 457 ests originating from three parasite developmental stages. twenty-two female-assoc ... | 2004 | 15478798 |
| involvement of central nervous system in the schistosomiasis. | the involvement of the central nervous system (cns) by schistosomes may or may not determine clinical manifestations. when symptomatic, neuroschistosomiasis (ns) is one of the most severe presentations of schistosomal infection. considering the symptomatic form, cerebral involvement is almost always due to schistosoma japonicum and the spinal cord disease, caused by s. mansoni or s. haematobium. available evidence suggests that ns depends basically on the presence of parasite eggs in the nervous ... | 2004 | 15486636 |
| the protective effect of a schistosoma japonicum chinese strain 23 kda plasmid dna vaccine in pigs is enhanced with il-12. | the schistosome integral membrane protein sm/sj23 was initially shown to induce protection in mice as a synthetic peptide vaccine and further, as a plasmid dna vaccine to induce protection in mice, sheep and water buffalo. in this study we asked if we could induce protection against challenge infection in pigs against schistosoma japonicum by vaccinating them with a plasmid dna vaccine encoding the s. japonicum chinese strain 23 kda membrane protein. further, we asked if we could enhance protect ... | 2004 | 15519710 |
| the fugitive ltr retrotransposon from the genome of the human blood fluke, schistosoma mansoni. | inspection of the nucleotide sequence of bacterial artificial chromosome number 49_j_14 [le paslier, m.c., pierce, r.j., merlin, f., hirai, h., wu, w., williams, d.l., johnston, d., loverde, p.t., le paslier, d., 2000. construction and characterization of a schistosoma mansoni bacterial artificial chromosome library. genomics 65, 87-94] from chromosome 1 of the genome of schistosoma mansoni (genbank ) revealed the likely presence of a proviral form of a novel schistosome retrotransposon. the nov ... | 2004 | 15542097 |
| the effect of colostrum on pigs pre-natally or post-natally exposed to schistosoma japonicum. | pre-natal infection of schistosoma japonicum in pigs may prove to be a useful model in shedding light on human pre-natal schistosomiasis. this study describes the effects of immune colostrum on worm burdens, tissue egg counts, liver pathology and crude worm or egg antigen-specific igg and iga responses, in groups of pigs pre-natally, pre-natally + post-natally or post-natally exposed to s. japonicum. results suggest that pre-natal exposure and immune colostrum did not affect the establishment of ... | 2004 | 15552404 |
| [risk factors associated with reinfection of schistosoma japonicum]. | to explore the risk factors of schistosoma japonicum reinfection after chemotherapy in a hilly endemic area. | 2004 | 15555394 |
| schistosoma japonicum: construction of phage display antibody library and its application in the immunodiagnosis of infection. | a monoclonal antibody would be an effective tool for the detection of circulating antigens in the serum of patients with schistosomiasis, but the traditional way of producing monoclonal antibodies is not cost-effective. the objective of this study was to find a new method for the large-scale production of monoclonal antibodies against schistosoma japonicum (sj). | 2004 | 15569489 |
| [analysis on morbidity and chemotherapy effects of schistosoma japonicum infection in fishermen on dongting lake]. | to clarify and evaluate the morbidity of schistosome infection and the effectiveness of chemotherapy among fishermen on east dongting lake. | 2004 | 15587151 |
| [the in silico elongation and analysis of the est from schistosoma japonicum]. | to construct a platform for in silico elongation and batch analysis of schistosoma japonicum (sj) ests, acquire the potential novel genes and research the expression profile of the genes. | 2004 | 15587155 |
| [identification of the source and types of cells of schistosoma japonicum by histochemical staining]. | to study the possibility on in vitro identifying the source or kinds of cells from schistosoma japonicum (s.j). | 2004 | 15587160 |
| [mucosal immunization of recombinant schistosoma japonicum ferritin]. | to subclone and express the gene encoding schistosoma japonicum ferritin (sjfer) and study its immune protection against challenge infection in mice vaccinated intranasally. | 2004 | 15597704 |
| [studies on smads at transcription level in liver fibrosis of mice with schistosomiasis japonica]. | to study smads involved in tgf-beta signal transduction at the transcription level during the development of liver fibrosis in balb/c mice infected by schistosoma japonicum. | 2004 | 15597710 |
| [expression of inducible nitric oxide synthase in the livers of mice infected with schistosoma japonicum]. | to study the expression of inducible nitric oxide synthase (inos) in livers of mice infected with schistosoma japonicum. | 2004 | 15597711 |
| [amplification and sequencing of the gene coding for mucin-like protein from schistosoma japonicum]. | to amplify and sequence the partial gene coding for mucin-like protein from chinese isolates of schistosoma japonicum (sjmlp). | 2004 | 15597714 |
| [prediction of the spatial distribution of oncomelania snails in marshland of jiangning county using the ordinary kriging]. | to explore the methods to predict the distribution of oncomelania hupensis in the marshland of jiangning county. | 2004 | 15597715 |
| immune responses against schistosoma japonicum after vaccinating mice with a multivalent dna vaccine encoding integrated membrane protein sj23 and cytokine interleukin-12. | the vaccination of mice with dna encoding single candidate antigens has failed to induce significant protection against schistosoma japonicum (s. japonicum) challenge infections. in this study, we evaluated the feasibility of using a multivalent dna vaccine which co-expressed s. japonicum integral membrane protein sj23 and murine cytokine il-12 to induce protective immune responses. | 2004 | 15603716 |
| [hbsag autologous red cell agglutination assay]. | three lot of hbsag autologous red cell agglutination kits (arca kit) have been qualified in lab, clinical trial and the stability confirmation have been performed. the sensitivity of the kits is 1ng/ml hbsag. a, b, o, ab type of peripheral blood or antiagglutinated specimens can be used for assay. no holk effect has been found. the repeated rate of 10 samples assayed for five times reached 100%. the specimens collected from hav, hcv, schistosoma japonicum infected patients or rf positive patient ... | 1997 | 15619914 |
| molecular evolutionary analyses implicate injection treatment for schistosomiasis in the initial hepatitis c epidemics in japan. | the mortality due to hepatocellular carcinoma (hcc) has ranged widely in various areas of japan since 30 years ago and the incidence was particularly high in once schistosoma japonicum (sj)-endemic areas. our aim was to estimate the spread time of hepatitis c virus (hcv) infection in the past with possible relevance to a higher incidence of hcc in once sj-endemic than sj-nonendemic areas. | 2005 | 15629506 |
| schistosoma japonicum: localization of calpain in the penetration glands and secretions of cercariae. | a monoclonal antibody was generated against the large subunit of schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. mice were immunized with recombinant s. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtained. in immunohistochemistry, a monoclonal antibody against s. japonicum calpain, kg-2e11, bound weakly to calpain expressed at the surface of adult worm tegument, however, it bound s ... | 2004 | 15639140 |
| testicular schistosomiasis mimicking tumour. | schistosomiasis or bilharziasis is a disease caused by schistosoma. when infecting men the most common parasites are schistosoma mansoni, schistosoma japonicum and schistosoma haematobium. the schistosoma mansoni is the only endemic parasite in brazil. we present a case of testicular schistosomiasis simulating malignancy. the case was treated successfully by excisional biopsy and praziquantel therapy. a review of the literature is discussed. | 2004 | 15663810 |
| crystallographic and thermodynamic analysis of the binding of s-octylglutathione to the tyr 7 to phe mutant of glutathione s-transferase from schistosoma japonicum. | glutathione s-transferases are a family of multifunctional enzymes involved in the metabolism of drugs and xenobiotics. two tyrosine residues, tyr 7 and tyr 111, in the active site of the enzyme play an important role in the binding and catalysis of substrate ligands. the crystal structures of schistosoma japonicum glutathione s-transferase tyrosine 7 to phenylalanine mutant [sjgst(y7f)] in complex with the substrate glutathione (gsh) and the competitive inhibitor s-octylglutathione (s-octyl-gsh ... | 2005 | 15667211 |
| gene cloning, expression and vaccine testing of schistosoma japonicum sjfabp. | a 600 bp dna fragment was amplified by pcr from an adult schistosoma japonicum cdna library. sequence analysis confirmed that this fragment contained an s. japonicum chinese mainland strain fatty acid binding protein (sj14fabp) gene. this gene was subsequently expressed in escherichia coli (e. coli) and in baculovirus/silkworm systems. the recombinant protein from e. coli was a 41 kda gst fusion protein (rsj14/gst), which could be purified by glutathione agarose affinity chromatography, with a y ... | 2004 | 15679632 |
| detection of s-glutathionylated proteins by glutathione s-transferase overlay. | oxidative and nitrosative stress lead to the s-glutathionylation of proteins and subsequent functional impairment. glutathione s-transferase (gst) from schistosoma japonicum was found to bind to the glutathione moiety of s-glutathionylated proteins, thus establishing a convenient method for detecting s-glutathionylated proteins by biotinylated gst. applications of this method to proteins that were prepared from cultured cells and blotted onto a membrane exhibited numerous positive bands, which w ... | 2005 | 15680905 |
| schistosomiasis control in china: the impact of a 10-year world bank loan project (1992-2001). | china has been carrying out large-scale schistosomiasis control since the mid-1950s, but in the early 1990s, schistosomiasis was still endemic in eight provinces. a world bank loan project enabled further significant progress to be made during the period 1992-2001. the control strategy was focused on the large-scale use of chemotherapy -- primarily to reinforce morbidity control -- while at the same time acting on transmission with the ultimate goal of interrupting it. chemotherapy was complemen ... | 2005 | 15682248 |
| the protective immunity of a dna vaccine encoding schistosoma japonicum chinese strain triose-phosphate isomerase in infected balb/c mice. | the development of a dna vaccine for schistosomiasis japonica and testing the protective efficacy after challenge in balb/c mice were performed. thirty-nine female balb/c mice were divided into three groups. each mouse of the control group was injected intramuscularly with 100 microg of pcdna3.1 dna. in the tpi group, each mouse was injected with 100 microg of pcdna3.1-sjctpi dna. the tpi+il-12 group was injected with 100 microg of pcdna3.1-sjctpi dna and 100 microg of the mixture of pcdna3.1-p3 ... | 2004 | 15689059 |
| proteomic analysis of differentially expressed proteins between the male and female worm of schistosoma japonicum after pairing. | identification of differentially expressed proteins between the male and female worm of schistosoma japonicum may provide new insights into the development of schistosomes, especially the molecular mechanism of female worm maturation induced by the male worm after pairing. comparative two-dimensional gel electrophoresis (2-de) and mass spectrometry were employed to separate and identify differentially expressed proteins between the male and female worm after pairing. soluble and hydrophobic prot ... | 2005 | 15700243 |
| a matched, case-control study of the association between schistosoma japonicum and liver and colon cancers, in rural china. | a study of liver and colon cancers and their association with a previous diagnosis of schistosomiasis was performed in rural sichuan, china. the data analysed came from 127 liver-cancer and 142 colon-cancer patients, each matched, by age, gender, hospital and township, with one or two controls. the cancer cases were identified from the hospital records of three sichuan counties (all of which have some level of endemic schistosomiasis japonicum): meishan (66 liver and 54 colon cases), pujiang (16 ... | 2005 | 15701255 |
| a bayesian-based approach for spatio-temporal modeling of county level prevalence of schistosoma japonicum infection in jiangsu province, china. | spatio-temporal variations of schistosoma japonicum infection risk in jiangsu province, china, were examined and the relationships between key climatic factors and infection prevalence at the county level were determined. the parasitological data were collected annually by means of cross-sectional surveys carried out in 47 counties from 1990 to 1998. climatic factors, namely land surface temperature (lst) and normalized difference vegetation index (ndvi), were obtained from remote sensing satell ... | 2004 | 15710436 |
| transport of schistosoma japonicum cercariae and the feasibility of niclosamide for cercariae control. | field experiments were conducted in southwestern china to estimate the distances over which cercariae of schistosoma japonicum are transported and remain infective in village irrigation ditches and to determine the feasibility of interrupting this transport using niclosamide, the molluscicide commonly used for snail control in this environment. the number of worms recovered from bioassay mice downstream from experimentally introduced cercariae, but without niclosamide, decreased about 90% over t ... | 2005 | 15710556 |
| expression of tgf-beta-like molecules in the life cycle of schistosoma japonicum. | the transforming growth factor beta (tgf-beta) family controls an extremely wide range of biological activities, such as the growth and differentiation of cells, and immunological events against infectious agents. although tgf-beta homologs appear to be widely present in metazoan animals, studies of parasite-derived molecules are relatively few. using antibodies against anti-mouse tgf-beta1, -beta2, and -beta3, we show the expression of tgf-beta-like molecules in schistosoma japonicum cercariae, ... | 2005 | 15711848 |
| schistosoma japonicum migration through mouse skin compared histologically and immunologically with s. mansoni. | the migration of schistosoma japonicum and s. mansoni through mouse skin epidermis and dermis was compared by immunofluorescence techniques from 4 to 22 h after infection. at all times, the percentage of parasites detected in the dermis was significantly higher for s. japonicum than for s. mansoni. thus, s. japonicum migrates more rapidly very early after infection. this agrees with the quicker migration observed previously by this species for later times. both species expressed antigens related ... | 2005 | 15723270 |
| an insight into the genetic variation of schistosoma japonicum in mainland china using dna microsatellite markers. | this study presents the first microsatellite investigation into the level of genetic variation among schistosoma japonicum from different geographical origins. s. japonicum isolates were obtained from seven endemic provinces across mainland china: zhejiang (jiashan county), anhui (guichi county), jiangxi (yongxiu county), hubei (wuhan county), hunan (yueyang area), sichuan 1 (maoshan county), sichuan 2 (tianquan county), yunnan (dali county), and also one province in the philippines (sorsogon). ... | 2005 | 15723675 |
| a multi-group model of schistosoma japonicum transmission dynamics and control: model calibration and control prediction. | previously we formulated a quantitative model to characterize site-specific schistosomiasis transmission. in this paper, we present a procedure to calibrate the model to data collected in endemic villages of south-western sichuan, china, with the objective of reducing parametric uncertainty to allow the model to describe local transmission with relative confidence. a bayesian approach using local epidemiological data and expert opinion is employed to calibrate the model. results indicate that, a ... | 2005 | 15730511 |
| schistosoma japonicum and occult blood loss in endemic villages in leyte, the philippines. | schistosoma japonicum has been related to anemia, but the mechanisms mediating this relationship remain unresolved. the primary objective of this study was to assess the role of occult blood loss in mediating s. japonicum-associated anemia after adjusting for age, sex, socioeconomic status (ses), and other helminth infections. the secondary objective was to identify intensity categories of risk for occult blood loss for trichuris and hookworm after adjustment for the presence of other helminth i ... | 2005 | 15741543 |
| cracks in the shell--zooming in on eggshell formation in the human parasite schistosoma mansoni. | schistosomiasis, currently the second most common parasitic disease of humans in tropical regions is caused by the eggs of trematode worms of the genus schistosoma. understanding egg formation and specifically the synthesis of the eggshell comprises, consequently, a promising starting point to cure and prevent the disease. to shed light on the genetics of the latter process, we analysed the three known s. mansoni eggshell proteins p14, p19 and p48 against the background of the species' inferred ... | 2005 | 15747129 |
| construction of a hepatic stellate cells subtracted cdna library of differentially expressed genes in normal mice and mice with schistosomiasis japonica. | to construct a hepatic stellate cells (hscs) subtracted cdna library to find differentially expressed genes in normal mice and mice infected with schistosoma japonicum (s. japonicum). suppression subtractive hybridization (ssh) was used. the cdna fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes. then differentially expressed cdna fragments were directly inserted into t/a cloning vector to set up the subtractive library. amplific ... | 2005 | 15754426 |
| establishment of schistosoma japonicum calpain-specific mouse t cell hybridomas and identification of a t cell epitope that stimulates ifngamma production. | calpain is a calcium-dependent cystein protease, and the homologues of schistosome are known as one of vaccine candidate molecules against schistosomiasis. here, we established two il-2 producing t cell hybridoma cell lines specific for schistosoma japonicum calpain, to identify t cell epitope(s) on the molecule. overlapping 15mer oligopeptides of calpain were synthesized and tested for their stimulatory abilities to the hybridomas. as a result, epitopes recognized by the two hybridoma lines wer ... | 2005 | 15780729 |
| gene transcription profile in mice vaccinated with ultraviolet-attenuated cercariae of schistosoma japonicum reveals molecules contributing to elevated ifn-gamma levels. | vaccination with ultraviolet-attenuated cercariae of schistosoma japonicum induced protective immunity against challenge infection in experimental animal models. our preliminary study on the transcription levels of ifn-gamma and il-4 in splenic cd4+ t cells revealed that attenuated cercariae elicited predominantly a th1 response in mice at the early stage, whereas normal cercariae stimulated primarily th2-dependent responses. further analysis on the gene profile of the skin-draining lymph nodes ... | 2005 | 15806292 |
| [distribution of inducible nitric oxide synthase in different stages of schistosoma japonicum]. | to study the presence of inos in schistosoma japonicum and demonstrate its distribution in different stages of this schistosome. | 2004 | 15830850 |
| [influence of interferon gamma treatment on expression of tgf-beta1 and its receptors in liver fibrosis of mice with schistosomiasis japonica]. | to investigate the effect of interferon-gamma (ifn-gamma) on the expression of tgf-beta1 and its two membrane receptors--tgf-beta receptor i (tbetari), tgf-beta receptor ii (tbetarii), and observe the expression of tgf-beta1, tbetari and tbetarii during the development of liver fibrosis in balb/c mice infected by schistosoma japonicum. | 2004 | 15830858 |
| [schistosome antibody detection by home microwave irradiation elisa]. | 2004 | 15830869 | |
| [prediction of the impact of climate warming on transmission of schistosomiasis in china]. | to predict the intensity and scale of impact on transmission of schistosomiasis japonica in china caused by the climate warming. | 2004 | 15830875 |
| [studies on the genetic variation of two mitochondrial dna molecules of schistosoma japonicum]. | to study the variation of schistosoma japonicum through two mitochondrial dna molecules. | 2004 | 15830887 |
| the cellular distribution and stage-specific expression of two dynein light chains from the human blood fluke schistosoma japonicum. | schistosomes are pathogenic helminth parasites of human portal veins. their body wall is a highly active syncytial tegument involved in an array of host interactions. the cytoskeletal organization and dynamics of this syncytium are poorly understood, but predominant motor components are the lc8 class of cytoplasmic dynein light chains (dlc). four lc8 members occur in schistosomes, two of which are expressed in the tegument. here, we describe the cytoplasmic distribution, stage-specific expressio ... | 2005 | 15833281 |
| comparison of skin invasion among three major species of schistosoma. | a comparison of the host-finding behavior, mode of skin invasion and skin-migratory patterns of the three major schistosomes of humans reveals major differences. among the three species, schistosoma japonicum is remarkable at conserving energy during the host-finding process, and exhibiting swift migration through the skin to reach the predilection site sooner and mature earlier compared with schistosoma mansoni and schistosoma haematobium. in this article, we summarize and compare the penetrati ... | 2005 | 15837605 |
| [vaccination of mice with recombinant nucleic acid vaccine encoding the integral membrane protein sj23 and cytokine il-12 elicits specific immune responses against schistosoma japonica]. | to develop a schistosoma japonica integral membrane protein sm23 or sj23 combined with murine il-12 dna-base vaccine against schistosomiasis. | 2005 | 15854467 |
| schistosoma japonicum reveals distinct reactivity with antisera directed to proteases mediating host infection and invasion by cercariae of s. mansoni or s. haematobium. | serine proteases released from the acetabular glands of cercariae, also known as cercarial elastases, are key enzymes in the penetration process of schistosomes through the skin of the final host. antisera against these enzymes secreted from schistosoma mansoni or s. haematobium reveal differences in the patterns of elastase expression among schistosome species and among different developmental stages of the larvae. immunolocalization studies showed that antisera raised against the enzyme s28 pr ... | 2005 | 15882236 |
| relationship between schistosoma japonicum and nutritional status among children and young adults in leyte, the philippines. | the objectives of this study were 1) to provide more accurate estimates of the relationship between schistosoma japonicum infection and both protein energy malnutrition (pem) and anemia through better adjustment for potential confounders such as socioeconomic status (ses) and geo-helminth infections and 2) to assess the role of occult blood loss in mediating s. japonicum-associated anemia. we examined cross-sectionally 729 individuals (86.7% s. japonicum-infected and 13.3% s. japonicum-uninfecte ... | 2005 | 15891125 |
| impact of parasitic infections and dietary intake on child growth in the schistosomiasis-endemic dongting lake region, china. | the simultaneous impacts of parasitic infections and dietary intake on growth patterns were investigated for 427 children aged 10-13 years in the schistosomiasis-endemic dongting lake region of china. height, weight, mid-upper arm circumference, and skinfold thicknesses (triceps, biceps, and subscapular) were measured, and eggs of schistosoma japonicum, ascaris lumbricoides, trichuris trichiura, and fasciolopsis buski in the collected stool samples of each subject were detected by kato-katz thic ... | 2005 | 15891126 |
| helminth infection and cognitive impairment among filipino children. | the objective of this study was to examine the independent effect of infection with each of four helminths (ascaris lumbricoides, schistosoma japonicum, necator americanus, and trichuris trichiura) on cognitive function after adjusting for the potential confounders nutritional status, socioeconomic status (ses), hemoglobin, sex, and the presence of other helminthes. this cross-sectional study was carried out in a rural village in leyte, the philippines in 319 children 7-18 years old. three stool ... | 2005 | 15891127 |
| schistosoma mekongi: a prominent neutrophil chemotactic activity of egg antigen with reference to that of schistosoma japonicum. | schistosoma mekongi causes granulomatous lesions around eggs deposited in the liver with neutrophil-rich inflammatory reactions in the early stage of the egg laying. to define the aspects of the typical pathogenesis of s. mekongi infection, we determined the difference between soluble egg antigen (sea) from s. mekongi and s. japonicum with a focus on chemotactic factors for neutrophils or eosinophils. mean volume and protein amount of s. mekongi eggs was 71 and 58% of those of schistosoma japoni ... | 2005 | 15893311 |
| [schistosomiasis transmission in urban area]. | 2004 | 15895491 | |
| comparative studies of the defense mechanism against schistosoma japonicum of schistosome-susceptible and -resistant oncomelania nosophora. | the amphibious snail oncomelania nosophora is an intermediate host of schistosoma japonicum. previously we reported that there are two strains of the snail, one resistant and one susceptible to a mindoro, the philippines, strain of s. japonicum. the resistant snails were collected from nirasaki and susceptible snails from kisarazu, japan. to determine early cellular responses in the two snail strains, we examined histologic alterations in the snails for up to 18 h after the initial exposure to m ... | 2005 | 15897002 |
| [a colloidal gold immunochromatographic assay for detecting p38 antigen of schistosome japonicum]. | to establish a colloidal gold immunochromatographic assay (gica) for detecting schistosome japonicum (sj). | 2005 | 15897128 |
| [cloning and characterization of new genes of schistosoma japonicum]. | to clone and characterize new genes of schistosoma japonicum, sj, and to provide efficient vaccine candidates. | 2005 | 15898426 |
| homology modeling and sar analysis of schistosoma japonicum cathepsin d (sjcd) with statin inhibitors identify a unique active site steric barrier with potential for the design of specific inhibitors. | proteases that digest the blood-meal of the parasitic fluke schistosoma are potential targets for therapy of schistosomiasis, a disease of chronic morbidity in humans. we generated a three-dimensional model of the cathepsin d target protease of schistosoma japonicum (sjcd) utilizing the crystal structure of human cathepsin d (hucd) in complex with pepstatin as template. a homology model was also generated for the related secreted aspartic protease 2 (sap2) of the pathogenic yeast, candida albica ... | 2005 | 15899696 |
| phage displaying peptides mimic schistosoma antigenic epitopes selected by rat natural antibodies and protective immunity induced by their immunization in mice. | to obtain the short peptides mimic antigenic epitopes selected by rat natural antibodies to schistosomes, and to explore their immunoprotection against schistosomiasis in mice. | 2005 | 15902737 |
| development of the rapid and simplified elisa (whole blood-elisa) using samples of schistosoma japonicum-infected human whole blood. | an elisa technique was developed using samples of schistosoma japonicum-infected human whole blood based on the conventional elisa. in this study, the following were demonstrated. 1) whole blood samples could be used. 2) the volume of whole blood and conjugate could be reduced to 0.05 ml. 3) the incubation time was shortened to 5 minutes. 4) the optical density could be measured at 10 minutes after transferring the substrate and the volume was reduced to 0.1 ml. 5) it did not require a fixed tem ... | 2005 | 15906645 |
| comparative study on rapid dot-immunogold staining and two immunogold silver staining assays for diagnosing schistosomiasis japonica. | a fast, specific, sensitive, convenient, and economical rapid-dot-immunogold staining (r-dot-igs) assay was used to detect serum antibodies in patients infected with schistosoma japonicum. the soluble egg antigen of schistosoma japonicum was added onto microspore membrane. after pre-reacting and blocking, the serum to be detected and sheep anti-human igg labeled with chloroauric acid were added sequentially. the assay took 15 minutes. for comparison, the dot-immunogold silver staining (dot-igss) ... | 2005 | 15906646 |
| hybridization of schistosoma mansoni and schistosoma japonicum in mice. | crossing experiments in mice with two human species of schistosoma japonicum (taiwan strain) and schistosoma mansoni (puerto rican strain) were performed. the hybrid miracidia from the cross between female s. japonicum and male s. mansoni infected both biophalaria glabrata and oncomalania h. chiui. however, those from the reciprocal crossing could infect only b. glabrata. b. glabrata infected with hybrid miracidia of female s. mansoni x male s. japonicum survived up to 30 days while those infect ... | 2005 | 15906648 |
| a rapid and simplified elisa using whole blood samples of schistosoma japonicum-infected rabbits. | a rapid and simplified elisa using whole blood samples of schistosoma japonicum-infected rabbits was compared with a conventional elisa. this whole-blood elisa has advantages. the volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. the incubation time was shortened to 5 minutes. wells were washed three to five times with pbs-tween after each procedure. optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. constant temperature wa ... | 2005 | 15916053 |
| [the use of unsupervised classification of landsat-5 tm images in analysing the types of vegetation in the areas of "breaking dikes or opening sluice for water store"]. | to clarify the change of vegetation types and its relationship between the density of alive-snails in the areas of "breaking dikes or opening sluice for water store" in jicheng. | 2005 | 15921606 |
| dose-dependent inhibition of gynecophoral canal protein gene expression in vitro in the schistosome (schistosoma japonicum) by rna interference. | the gynecophoral canal protein gene sjgcp of schistosoma japonicum that is necessary for the pairing between the male and female worms is specifically expressed in the adult male worm. this protein is widely distributed in the adult female worm after pairing. reverse transcription-polymerase chain reaction (rt-pcr) and immunofluorescence were employed to analyze the relationship between the rnai effect and dsrna dosage in the parasites. the results revealed that the inhibition of sjgcp expressio ... | 2005 | 15944753 |
| characterization of a novel vaccine candidate and serine proteinase inhibitor from schistosoma japonicum (sj serpin). | serine proteinase inhibitors (serpins) represent an important superfamily of endogenous inhibitors that regulate proteolytic events active in a variety of physiological functions. immunological screening of a schistosoma japonicum adult worm cdna expression library with sera of microtus fortis, a naturally resistant vertebrate host, has identified one clone that encoded for a sequence homologous to those of the serpin superfamily. the full-length sequence encoding s. japonicum serpin (sj serpin) ... | 2005 | 15946799 |
| multiple cathepsin b isoforms in schistosomula of trichobilharzia regenti: identification, characterisation and putative role in migration and nutrition. | among schistosomatids, trichobilharzia regenti, displays an unusual migration through the peripheral and central nervous system prior to residence in the nasal cavity of the definitive avian host. migration causes tissue degradation and neuromotor dysfunction both in birds and experimentally infected mice. although schistosomula have a well-developed gut, the peptidases elaborated that might facilitate nutrition and migration are unknown. this is, in large part, due to the difficulty in isolatin ... | 2005 | 15950230 |
| estimating and modeling the dynamics of the intensity of infection with schistosoma japonicum in villagers of leyte, philippines. part ii: intensity-specific transmission of s. japonicum. the schistosomiasis transmission and ecology project. | a dynamic model of schistosoma japonicum transmission is presented that incorporates effects of infection intensity, age, and sex. we use four infection intensity classes to investigate the impact of ecologic changes and public health interventions on the burden of infection within communities. age- and sex-specific infection data from three disease-endemic villages in the philippines are used to estimate the parameters of the model. the model gives good qualitative agreement with observed fecal ... | 2005 | 15964960 |
| estimating the intensity of infection with schistosoma japonicum in villagers of leyte, philippines. part i: a bayesian cumulative logit model. the schistosomiasis transmission and ecology project (step). | intensity profiles for helminths are used to describe population infection status, monitor effectiveness of control programs, and provide accurate data to validate transmission models. this study aims to accurately predict age/gender specific intensity profiles of endemic schistosomiasis japonica infection in the philippines. poor sensitivity of the kato-katz test and large heterogeneity in infection levels across villages complicate these predictions. data from 1,989 individuals living in three ... | 2005 | 15967758 |
| comparative studies on susceptibilities of two different japanese isolates of oncomelania nosophora to three strains of schistosoma japonicum originating from japan, china, and the philippines. | oncomelania nosophora (gastropoda: pomatiopsidae) is the intermediate host of schistosoma japonicum in japan. although most of the snails were eliminated during the 20th century, they are still found in two areas in japan. one area is in the kofu basin, including nirasaki city, in yamanashi prefecture. the other is the obitsu river basin in kisarazu city, chiba prefecture. snails collected in nirasaki and kisarazu were exposed to 3 geographical strains of s. japonicum originating from japan, chi ... | 2005 | 15991496 |
| nutritional status and serum cytokine profiles in children, adolescents, and young adults with schistosoma japonicum-associated hepatic fibrosis, in leyte, philippines. | in a cross-sectional study of 641 schistosoma japonicum-infected individuals in leyte, philippines, who were 7-30 years old, we determined the grade of hepatic fibrosis (hf) by ultrasound and used anthropometric measurements and hemoglobin levels to assess nutritional status. serum levels of interleukin (il)-1, il-6, and il-10; tumor-necrosis factor (tnf)-alpha; soluble tnf- alpha receptor i; and c-reactive protein (crp) were measured to examine the association between these markers of inflammat ... | 2005 | 15995969 |