Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| involvement of dsrna virus in the protein composition and growth kinetics of host trichomonas vaginalis. | trichomonas vaginalis harbors a double-stranded (ds)-rna virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (khoshnan a, alderete jf (1994) j virol 68: 4035-4038). to further test the influence of virus on t. vaginalis, virus-infected (v+) isolates were compared to virus-free (v-), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. comparative high re ... | 1997 | 9191859 |
| chromosome number of trichomonas vaginalis. | chromosome number of an axenically grown trichomonas vaginalis isolate was studied using a 1 mm solution of colchicine and a hypotonic swelling technique. the diploid chromosome number was 2n = 6 [corrected]. each pair of chromosomes can be identified by its morphology and size. this observation could be important with respect to gene mapping and molecular cloning for genes of t. vaginalis. | 1997 | 9194850 |
| a self-administered technique for the detection of sexually transmitted diseases in remote communities. | the control of sexually transmitted diseases (stds) in remote rural communities would be enhanced by a sensitive self-administered method for the detection of asymptomatic infection. results of conventional methods for the detection of stds were compared with results of tampon-collected specimens analyzed by polymerase chain reaction (pcr) for 480 women. neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis were detected by routine methods in 4 (1%), 14 (3%), and 41 (9%) sample ... | 1997 | 9207385 |
| arsenical pessaries in the treatment of metronidazole-resistant trichomonas vaginalis. | 1997 | 9228600 | |
| molecular approaches to the diagnosis of male infertility. | the introduction of the polymerase chain reaction (pcr) as a technique to selectively amplify and identify specific dna and rna sequences has revolutionized the field of molecular medicine. application of these newly developed molecular techniques to the field of male infertility has made the delineation of subtle causes of infertility-an inapparent genital tract infection, immune system activation within the genital tract, mutations in sperm mitochondrial or chromosomal dna, alterations in sper ... | 1996 | 9238679 |
| synthesis and antiparasitic activities of amidinic azolated derivatives. | a set of heterocyclic n-acetamidinium hydrochlorides were prepared from the corresponding n-acetonitriles. the antiparasitic screening showed that, while all amidines are practically inactive, some nitriles present leishmanicide properties. | 1997 | 9241827 |
| conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes crithidia, trypanosoma and trichomonas. | kinetoplastid protozoa are the earliest-branching eukaryotes to possess a true mitochondrion. this organelle is host to a variety of intriguing and unique features, including rna editing. we examined the characteristics of protein import into mitochondria of trypanosoma brucei. dihydrofolate reductase (dhfr) carrying a yeast mitochondrial targeting signal was correctly translocated into trypanosome mitochondria in vivo, as were dhfr fusion proteins bearing two unusually short (7-9 amino acids) p ... | 1997 | 9243185 |
| trichomonas vaginalis associated with low birth weight and preterm delivery. the vaginal infections and prematurity study group. | several studies have suggested that pregnant women infected with trichomonas vaginalis may be at increased risk of an adverse outcome. | 1997 | 9243743 |
| evidence for loss of mitochondria in microsporidia from a mitochondrial-type hsp70 in nosema locustae. | in molecular phylogenies based on ribosomal rna, three amitochondriate protist lineages, microsporidia, metamonada (including diplomonads) and parabasala (including trichomonads), are the earliest offshoots of the eukaryotic tree. as an explantation for the lack of mitochondria in these organisms, the hypothesis that they have diverged before the mitochondrial endosymbiosis is preferred to the less parsimonious hypothesis of several independent losses of the organelle. nevertheless, if they had ... | 1997 | 9247927 |
| extracellular release by trichomonas vaginalis of a nadp+ dependent malic enzyme involved in pathogenicity. | this report presents evidence showing that trichomonas vaginalis releases in the extracellular environment a functional form of nadp(+)-dependent malic enzyme. the protein which is likely responsible for the oxidative decarboxilase activity had already been identified in previous studies as p65, one of the five adhesive proteins of the protozoan. the same protein had also been described as ap65 by other authors, which identified it as one of the four surface proteins specifically responsible for ... | 1997 | 9250781 |
| molecular cloning of actin genes in trichomonas vaginalis and phylogeny inferred from actin sequences. | the parasitic protozoan trichomonas vaginalis is known to contain the ubiquitous and highly conserved protein actin. a genomic library and a cdna library have been screened to identify and clone the actin gene(s) of t. vaginalis. the nucleotide sequence of one gene and its flanking regions have been determined. the open reading frame encodes a protein of 376 amino acids. the sequence is not interrupted by any introns and the promoter could be represented by a 10 bp motif close to a consensus mot ... | 1997 | 9252588 |
| a rapid diagnostic test for trichomonas vaginalis infection. | the applicability of the quantitative buffy coat tube test (qbc) for diagnosis of t. vaginalis was studied. the results of the qbc tube test was compared with that of the wet mount examination in 60 women having genital infection. the cases were selected from outpatient clinic of el-shatby university hospital t. vaginalis was identified by qbc tube test in 24 cases out of 60 while the wet mount smear, it was detected in 21 cases. the sensitivity of the qbc tube test was 100% and the specificity ... | 1997 | 9257972 |
| isolation and characterization of cytosolic malate dehydrogenase from trichomonas vaginalis. | malate dehydrogenase (ec 1.1.1.37.) (mdh) was purified to apparent homogeneity from the cytosolic fraction of the protozoan trichomonas vaginalis donné. the four step purification included ion-exchange chromatography (deae-sephacel and q-sepharose, elution with nacl) and affinity chromatography (reactive red agarose, elution with nadh and nacl). the enzyme was purified about 132-fold (30.6% yield) to a specific activity of 352 units mg-1. the km values determined at ph 7.8 (ph optimum from 7.5 t ... | 1997 | 9269720 |
| study on ultrastructural cytochemistry and pathogenic mechanism of trichomonas vaginalis. | to study the relation among enzymic ultrastructural localizations, cell organelles and functions of trichomonas vaginalis (t. vaginalis) as well as its pathogenic mechanism. | 1996 | 9275338 |
| tampon sampling for diagnosis of bacterial vaginosis: a potentially useful way to detect genital infections? | genital tract infections are important causes of ill health in developing countries, but diagnosis is difficult. bacterial vaginosis (bv) was correctly diagnosed by using a vaginal specimen obtained by tampon sampling in 22 of 24 women (91.6%) for whom bv was diagnosed by gram staining. the yield for other vaginal infections was higher (28% for trichomonas vaginalis and 32.7% for candida albicans) than it was for cervical infections (0% for neisseria gonorrhoeae and 30% for chlamydia trachomatis ... | 1997 | 9276426 |
| hiv-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. | in-vitro research has suggested that bacterial vaginosis may increase the survival of hiv-1 in the genital tract. therefore, we investigated the association of hiv-1 infection with vaginal flora abnormalities, including bacterial vaginosis and depletion of lactobacilli, after adjustment for sexual activity and the presence of other sexually transmitted diseases (stds). | 1997 | 9284776 |
| trichomonas vaginalis: analysis of codon usage. | 1997 | 9287961 | |
| the relationship of bacterial vaginosis, candida and trichomonas infection to symptomatic vaginitis in postmenopausal women attending a vaginitis clinic. | to estimate the prevalence of bacterial vaginosis, candida albicans, and trichomonas vaginalis infections in a population of postmenopausal women with symptoms of vaginitis seen at a vaginitis clinic either as self-referred or clinician referred patients. | 1997 | 9288698 |
| [the microbial etiology of genital discharges in nouakchott, mauritania]. | the survey carried on 579 patients of both sexes, consulting for genital discharges (spontaneous or referred), showed that in 61.1% men cases, std agents were isolated (neisseria gonorrhoeae 51.5%) and 64.4% in women cases (gardnerella vaginalis 24%, candida albicans 20%. trichomonas vaginalis 14%). 17% of neisseria gonorrhoeae strains were penicillinase-producing (ppng). | 1997 | 9289258 |
| use of antibiotics to prevent preterm birth. | our purpose is to review recent data and provide a clinical opinion on the use of antibiotics to prevent preterm birth or related maternal-neonatal complications. a literature review and a synthesis of opinion are provided. during prenatal care, standard practices should be applied regarding neisseria gonorrhoeae, chlamydia trachomatis, and bacteriuria. in addition, screen for and treat bacterial vaginosis in patients at high risk for preterm birth but do not treat ureaplasma urealyticum or grou ... | 1997 | 9290454 |
| colpitis macularis and macular vaginitis unrelated to trichomonas vaginalis infection. | 1997 | 9292350 | |
| evaluation of a murine model of experimental trichomoniasis. | by using a reference strain of trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. our results suggest that the inoculation of balb/c mice with 10(7) trichomonads and the semiquantitative assessment at day 15 postinfection of the gross-pathologic changes in the abdominal cavity--pe ... | 1997 | 9296057 |
| molecular characterization of a sarcoplasmic-endoplasmic reticulum ca+2 atpase gene from trichomonas vaginalis. | dna fragments homologous to p-type cation translocating atpase genes were identified in trichomonas vaginalis by polymerase chain reaction (pcr) amplification. the genomic locus corresponding to one pcr fragment, tvca1, contains a 3,055 base-pair open reading frame encoding a 108,162 dalton protein composed of 981 amino acids. tvca1 lacks introns, is present in a single copy, and is expressed as a 3.1 kb transcript with short 5' and 3' untranslated regions. separate primer extension experiments ... | 1997 | 9304817 |
| hormonal contraception, vitamin a deficiency, and other risk factors for shedding of hiv-1 infected cells from the cervix and vagina. | factors that influence shedding of hiv-1 infected cells in cervical and vaginal secretions may be important determinants of sexual and vertical transmission of the virus. we investigated whether hormonal contraceptive use, vitamin a deficiency, and other variables were risk factors for cervical and vaginal shedding of hiv-infected cells. | 1997 | 9314871 |
| the role of nitric oxide as an effector of macrophage-mediated cytotoxicity against trichomonas vaginalis. | the purpose of this study is to determine whether nitric oxide is involved in the extracellular killing of trichomonas vaginalis by mouse (balb/c) peritoneal macrophages and raw264.7 cells activated with lps or rifn-gamma and also to observe the effects of various chemicals which affect the production of reactive nitrogen intermediates (rni) in the cytotoxicity against t. vaginalis. the cytotoxicity was measured by counting the release of [3h]-thymidine from labelled protozoa and no2- was assaye ... | 1997 | 9335184 |
| trichomoniasis in a postmenopausal woman cured after discontinuation of estrogen replacement therapy. | epidemiologic and experimental data suggest that estrogen has a salutary effect on trichomonas vaginalis. | 1997 | 9339974 |
| evolution of the hydrogenosome. | since its discovery almost 25 years ago the enigmatic hydrogenosome, a redox organelle of anaerobic unicellular eukaryotes, has puzzled evolutionists as to its origin and function. synthesis of recent molecular, physiological and morphological studies now favours the hypothesis that hydrogenosomes derived from a modification of pre-existing mitochondria, and argues against the previously held view that the hydrogenosome had a polyphyletic origin. these data provide evidence for a more ancient or ... | 1997 | 9351194 |
| metronidazole. a therapeutic review and update. | the nitroimidazole antibiotic metronidazole has a limited spectrum of activity that encompasses various protozoans and most gram-negative and gram-positive anaerobic bacteria. metronidazole has activity against protozoans like entamoeba histolytica, giardia lamblia and trichomonas vaginalis, for which the drug was first approved as an effective treatment. anaerobic bacteria which are typically sensitive are primarily gram-negative anaerobes belonging to the bacteroides and fusobacterium spp. gra ... | 1997 | 9360057 |
| entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agents. | pyrophosphate-dependent phosphofructokinase (ppi-pfk) is the rate-limiting glycolytic enzyme found in the pathogenic protists entamoeba histolytica, giardia lamblia, toxoplasma gondii, trichomonas vaginalis, and naegleria fowleri. the enzyme differs significantly from atp-dependent phosphofructokinases found in humans and as such represents an important drug target. current therapy for infections caused by these pathogens is inadequate, especially for children, pregnant women, and the immune com ... | 1997 | 9371084 |
| the vagina has reducing environment sufficient for activation of trichomonas vaginalis cysteine proteinases. | trichomonas vaginalis, a worldwide distributed sexually transmitted protozoan, is remarkable for synthesis of numerous, distinct cysteine proteinases, the significance of which is evidenced by the presence in vivo of soluble proteinases in secretions and antiproteinase antibody in serum of patients with trichomonosis. these proteinases purportedly play a role in host parasitism and immune evasion. | 1997 | 9389953 |
| a comparison of the sensitivity of the inpouch tv, diamond's and trichosel media for detection of trichomonas vaginalis. | this study compared the ability of three culture media (inpouch tv, diamond's, and trichosel) to support the growth of clinical isolates of trichomonas vaginalis and their relative sensitivity for detection of the organism. | 1997 | 9389954 |
| contact-dependent disruption of the host cell membrane skeleton induced by trichomonas vaginalis. | this report presents evidence showing that the pathogenetic process of the protozoan parasite trichomonas vaginalis involves degradation of the target cell membrane skeleton; spectrin, the most representative protein within this structure, has been identified as the main molecular target. degradation of the target cell spectrin is accomplished only upon contact with the parasite, and immunochemical and immunofluorescence studies performed with the erythrocyte as a model demonstrate that degradat ... | 1997 | 9393808 |
| molecular methods for diagnosis and epidemiological studies of parasitic infections. | direct microscopy is widely used for the diagnosis of parasitic infections although it often requires an experienced microscopist for accurate diagnosis, is labour intensive and not very sensitive. in order to overcome some of these shortcomings, molecular or nucleic acid-based diagnostic methods for parasitic infections have been developed over the past 12 years. the parasites which have been studied with these techniques include the human plasmodia, leishmania, the trypanosomes, toxoplasma gon ... | 1997 | 9394184 |
| comparison of the inpouch tv culture system and diamond's modified medium for detection of trichomonas vaginalis. | this study compared the use of diamond's modified medium to inpouch for the culture of trichomonas vaginalis from pooled vaginal secretions. the sensitivity for inpouch was 82.4% (61/74) versus 87.8% (65/74) for diamond's modified medium. there were no significant differences in the sensitivity and negative predictive value of inpouch compared to diamond's modified medium. | 1997 | 9399542 |
| [vulvovaginitis]. | candidiasis, infection due to trichomonas vaginalis and bacterial vaginosis (gardnerella vaginalis and/or other species) represent the major three causes of vulvo-vaginitis. other are rare bacterial infections and non infectious vaginitis such as allergic and post-menopausal vaginitis with epithelial atrophy. clues for the diagnosis include the clinical features of vaginal discharge, cytological examinations, bacterial and fungal cultures. only t. vaginalis seems to be responsible of sexually tr ... | 1997 | 9406511 |
| biochemical properties of a neuraminidase of trichomonas vaginalis. | trichomonas vaginalis possesses a membrane-associated neuraminidase activity that is released into culture medium during its growth in vitro. the neuraminidase shows an optimum ph of 4.5 and a km of 0.15 mm for 2'-(4-methylumbelliferyl)-alpha-d-n-acetyl-neuraminic acid as a substrate. this enzyme releases mainly alpha-2,3-linked sialic acid because it is able to liberate sialic acid from sialyllactose (mainly alpha-2,3) but not from mucin (alpha-2,6) or fixed erythrocytes (mainly alpha-2,6). the ... | 1997 | 9406769 |
| incidence of trichomonas vaginalis among women having vaginal discharge, in manisa, turkey. | trichomoniasis characterise with a foamy yellowish odorous discharge, is an infection that causes superficial defects and necrotic ulcers in mucosa, and is spreadable with sexual contact. in t. vaginalis infection, the diagnosis can be made after the examination of saline wet mount preparations, stained smears preparation and cultivation of the vaginal discharge, the urine and the materials obtained from prostate secretion in males. in the present study, vaginal discharge samples obtained from 2 ... | 1997 | 9425833 |
| managing vaginal trichomoniasis resistant to high-dose metronidazole therapy. | 1997 | 9433954 | |
| characterization of trichomonad species and strains by pcr fingerprinting. | the random amplified polymorphic dna (rapd) technique was used for phylogenetic analysis of trichomonads, for intraspecies genealogical study of trichomonas vaginalis strains, and for assessment of intrastrain polymorphism in trichomonas vaginalis. the phylogenetic tree for 12 trichomonad species showed certain discrepancies with current models of trichomonad evolution. however, it shows that rapd traits retain phylogenetically relevant information. the results of intraspecies analyses of 18 tri ... | 1997 | 9435127 |
| [comparative study for the evaluation of the efficacy and safety of metronidazole and secnidazole in the treatment of vaginal trichomoniasis]. | presently study included 3 groups of 20 women with tricomoniasis demonstrated parasitologicaly for oozing vaginal, the first group received treatment with vaginal ova with metronidazol for 10 days, the second group ova of secnidazol for 3 days and the third group ova of secnidazol for 7 days. the results showed that the clinical manifestations dimanished significantly and the presence of trichomonas vaginalis disappeared from the vaginal cavity, therefore cure was achieved parasitological in all ... | 1997 | 9441152 |
| trichomonas vaginalis and amplification of hiv-1 transmission. | 1998 | 9449891 | |
| only two of the trichomonas vaginalis triplet ap51 adhesins are regulated by iron. | the sexually transmitted parasite trichomonas vaginalis cytoadheres to the vaginal epithelium, and four candidate trichomonad adhesins have been identified. one such protein, termed ap51, was characterized further. to do this, we studied a 1 kb cdna clone (ap51.2) isolated from a phagemid expression library, which encoded a fusion protein of approximately 38 kda that was immuno-crossreactive with anti-ap51 serum and retained functional adhesive properties. we performed 5'-pcr amplification to re ... | 1998 | 9466942 |
| [inflammatory smears in cervicovaginal cytology. a finding meaning infection?]. | inflammatory changes are relatively frequent findings in cervical smears and are generally believed to be a consequence of genital infection. however, clinical signs of infection are frequently absent and no consensus exists on the management of these patients. the objective of this study was to assess whether or not inflammatory smears are exclusively a consequence of genital infection. for this purpose, the prevalence of genital infection in a group of women with inflammatory cells in the cerv ... | 1997 | 9477586 |
| the primitive protozoon trichomonas vaginalis contains two methionine gamma-lyase genes that encode members of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes. | methionine gamma-lyase, the enzyme that catalyzes the breakdown of methionine by an alpha,gamma-elimination reaction and is a member of the gamma-family of pyridoxal 5'-phosphate-dependent enzymes, is present in high activity in the primitive protozoan parasite trichomonas vaginalis but is absent from mammals. two genes, mgl1 and mgl2, encoding methionine gamma-lyase, have now been isolated from t. vaginalis. they are both single copy, encode predicted proteins (mgl1 and mgl2) of 43 kda, have 69 ... | 1998 | 9488680 |
| sexually transmitted diseases in adolescents: what's new? | sexually transmitted diseases (stds) are an important cause of morbidity and, potentially, mortality for adolescents in the united states. each year, 2.5 million teenagers in this country will contract an std. an understanding of recent advances in risk factors, epidemiology, diagnosis, and treatment of these important infections is vital for clinicians providing care for adolescents. | 1998 | 9496862 |
| epidemiology and outcomes associated with moderate to heavy candida colonization during pregnancy. vaginal infections and prematurity study group. | our purpose was to determine the risk factors, physical findings, microflora, and pregnancy outcome among pregnant women with moderate to heavy vaginal growth of candida albicans and other candida species. | 1998 | 9500502 |
| diagnosis of trichomonas vaginalis in adolescent females: inpouch tv culture versus wet-mount microscopy. | this study compared the inpouch tv culture to wet-mount, diamond's culture medium, and papanicolaou (pap) smear for the diagnosis of trichomonas infection in sexually active adolescents. | 1998 | 9502007 |
| cloning, sequencing and expression of the acylneuraminate lyase gene from clostridium perfringens a99. | the acylneuraminate lyase gene from clostridium perfringens a99 was cloned on a 3.3 kb hindiii dna fragment identified by screening the chromosomal dna of this species by hybridization with an oligonucleotide probe that had been deduced from the n-terminal amino acid sequence of the purified protein, and another probe directed against a region that is conserved in the acylneuraminate lyase gene of escherichia coli and in the putative gene of clostridium tertium. after cloning, three of the recom ... | 1997 | 9511987 |
| glycosidases in mucin-dwelling protozoans. | a range of protozoans were tested for the presence of glycosidases using p-nitrophenyl sugars as substrates. some of the organisms were mucin dwellers whereas others were blood borne parasites. it had been hypothesized that glycosidase production would be significantly higher in the mucin dwellers. the results obtained demonstrated that the urogenital protozoans tritrichomonas foetus and trichomonas vaginalis produced a vast range of glycosidases which included those required for mucin breakdown ... | 1997 | 9511996 |
| the relationship of cocaine use and human immunodeficiency virus serostatus to incident sexually transmitted diseases among women. | to assess the incidence of sexually transmitted diseases (std) in a group of heterosexual women as a function of human immunodeficiency virus (hiv) serostatus and to ascertain the effect of crack cocaine use on these relationships. | 1998 | 9518381 |
| observation of membrane fusion on the interaction of trichomonas vaginalis with human vaginal epithelial cells. | the in vitro cytopathic effect of trichomonas vaginalis on epithelial cells was analyzed through the interaction of two parasite strains with freshly collected human vaginal epithelial cells (hvecs) from normal women. videomicroscopy, light and electron microscopy (scanning and transmission), freeze-fracture, the tracer lanthanum nitrate, and the periodic acid-thio-semicarbazide-silver proteinate techniques were used to analyze regions of close contact between the hvecs and t. vaginalis. after 2 ... | 1998 | 9521011 |
| prevalence of vaginal infections with bacterial vaginosis, trichomonas vaginalis and candida albicans among pregnant women at the port moresby general hospital antenatal clinic. | a clinico-sociodemographic and microbiological survey was carried out at the port moresby general hospital antenatal clinic to determine the prevalences of bacterial vaginosis, trichomonas vaginalis and candida albicans vaginal infections in pregnancy and to examine if the infections had any association with some suspected sociodemographic risk factors. the study was carried out between december 1990 and january 1991. of 206 consecutive subjects surveyed, 79 (38%) had symptomatic infection. howe ... | 1995 | 9522855 |
| a pilot study of metronidazole vaginal gel versus oral metronidazole for the treatment of trichomonas vaginalis vaginitis. | trichomonas vaginalis is a common sexually transmitted pathogen. in the united states, oral metronidazole is the only officially sanctioned treatment option. | 1998 | 9524997 |
| female reproductive tract immunity in bovine trichomoniasis. | mechanisms of protective immunity in the female reproductive tract are poorly understood. for sexually transmitted diseases, bovine trichomoniasis is a useful model because it resembles human trichomoniasis to some extent, and antibodies play an important role in protection against these extracellular parasites. protective efficacy was compared in animals with genital responses of predominantly immunoglobulin g (igg) or predominantly iga antibodies to a purified surface antigen of tritrichomonas ... | 1998 | 9526608 |
| collaborative approach to improve the detection and management of trichomoniasis in a low prevalence district. | we describe a simple collaborative approach developed by the departments of cytology, microbiology and genitourinary (gu) medicine for the detection, diagnosis and management of microbiologically confirmed trichomonas vaginalis (tv) infection. over a 2-year period, 54 (0.1%) of 52,440 cervical smears were reported to show tv, but microbiological confirmation was made in only 76% of 34 patients from whom a vaginal swab was subsequently taken. trichomoniasis should not be diagnosed by cytology alo ... | 1998 | 9530903 |
| a population based case-control teratologic study of oral metronidazole treatment during pregnancy. | to study human teratogenic risk of metronidazole. | 1998 | 9532994 |
| molecular analysis of the gene encoding the immunodominant phenotypically varying p270 protein of trichomonas vaginalis. | trichomonas vaginalisis a flagellated protozoan responsible for the most common non-viral sexually transmitted disease. the immunogen p270 was previously found to be up-regulated in expression and to undergo phenotypic variation between surface versus cytoplasmic localization in trichoimonads harbouring a dsrna virus. in this report, we characterize the entire p270 open reading frame (orf) and the unknown flanking 5;- and 3;-unique, non-repeat coding sequences of the gene in addition to untransl ... | 1998 | 9533894 |
| genital chlamydia trachomatis (serotypes d-k) infection in jamaican commercial street sex workers. | to determine the prevalence of genital chlamydia trachomatis infections in commercial street sex workers (cssw) in jamaica. | 1997 | 9534744 |
| cloning and expression of an iron-containing superoxide dismutase in the parasitic protist, trichomonas vaginalis. | a superoxide dismutase (sod) gene of the parasitic protist trichomonas vaginalis was cloned, sequenced, expressed in escherichia coli, and its gene product characterized. it is an iron-containing dimeric protein with a monomeric mass of 22,067 da. southern blots analyses suggested the presence of seven iron-containing (fesod) gene copies. hydrophobic cluster analysis revealed some peculiarities in the 2d structure of the fesod from t. vaginalis and a strong structural conservation between prokar ... | 1998 | 9561738 |
| yigsr is the preferential laminin-1 residing adhesion sequence for trichomonas vaginalis. | 1998 | 9562428 | |
| clinical and microbiological aspects of trichomonas vaginalis. | trichomonas vaginalis, a parasitic protozoan, is the etiologic agent of trichomoniasis, a sexually transmitted disease (std) of worldwide importance. trichomoniasis is the most common nonviral std, and it is associated with many perinatal complications, male and female genitourinary tract infections, and an increased incidence of hiv transmission. diagnosis is difficult, since the symptoms of trichomoniasis mimic those of other stds and detection methods lack precision. although current treatmen ... | 1998 | 9564565 |
| utilisation of genitourinary medicine services by general practitioners: effect of geographic location, fundholding status, and potential effect of total fundholding. | to determine the current utilisation of genitourinary medicine (gum) services by general practitioners (gps) and any changes likely to occur with moves towards a primary care led nhs. | 1997 | 9582477 |
| sexually transmitted disease among married zambian women: the role of male and female sexual behaviour in prevention and management. | few studies have evaluated the relation between male and female sexual behaviour and std among married african women. the objectives of this study were to identify male and female sexual behaviour associated with female std, and to explore whether incorporating male and female sexual behaviour and male symptoms can improve algorithms for std management in married african women. | 1997 | 9582483 |
| family planning services in developing countries: an opportunity to treat asymptomatic and unrecognised genital tract infections? | to determine the prevalence of asymptomatic and unrecognised genital tract infections among women attending a family planning clinic in rural south africa. | 1997 | 9582484 |
| cure of metronidazole- and tinidazole-resistant trichomoniasis with use of high-dose oral and intravaginal tinidazole. | 1998 | 9597267 | |
| involvement of trichomonas vaginalis surface-associated glycoconjugates in the parasite/target cell interaction. a quantitative electron microscopy study. | cytochemical labeling with gold particle-conjugated lectins in combination with transmission and scanning electron microscopy was used to localise specific sugar residues on the trichomonas vaginalis cell surface. for investigation of the role played by the surface glycoconjugates of t. vaginalis in the process of parasite adhesion to the target cells, selected glycan moieties of parasite surface-bound molecules were removed by treatment with alpha-mannosidase and beta-n-acetylglucosaminidase. f ... | 1998 | 9610634 |
| evaluation and management of vaginitis. | to evaluate recent advances in our understanding of the clinical relevance, diagnosis, and treatment of vaginal infections, and to determine an efficient and effective method of evaluating this clinical problem in the outpatient setting. | 1998 | 9613891 |
| study of trichomoniasis among egyptian male patients. | in the present study, trichomonas vaginalis was diagnosed in 28.8% male patients with urethral discharge and in 8.2% suffering from impotence and infertility. diagnosis was based on examination of urethral discharge, urine, semen and prostatic massage fluid by wet mount, stained films and culture inoculation. diamond's culture proved to be the method of choice. | 1998 | 9617063 |
| secondary absence of mitochondria in giardia lamblia and trichomonas vaginalis revealed by valyl-trna synthetase phylogeny. | nuclear-coded valyl-trna synthetase (valrs) of eukaryotes is regarded of mitochondrial origin. complete valrs sequences obtained by us from two amitochondriate protists, the diplomonad, giardia lamblia and the parabasalid, trichomonas vaginalis were of the eukaryotic type, strongly suggesting an identical history of valrs in all eukaryotes studied so far. the findings indicate that diplomonads are secondarily amitochondriate and give further evidence for such conclusion reached recently concerni ... | 1998 | 9618503 |
| three genes encode distinct ap33 proteins involved in trichomonas vaginalis cytoadherence. | adherence to host cells is essential for the initiation and maintenance of infection by mucosal pathogens. the protozoan trichomonas vaginalis colonizes the human urogenital tract via four surface proteins (ap65, ap51, ap33 and ap23). to characterize ap33 further, six cdna clones were examined. restriction mapping indicated that the six clones represented three similar genes. southern analysis confirmed the existence of three single-copy ap33 genes and suggested a semi-conservative genomic arran ... | 1998 | 9622355 |
| clinical findings in female genital schistosomiasis in madagascar. | to assess the morbidity of s. haematobium infection in women of reproductive age (15-49 years) in the western part of madagascar, the village of betalatala with a prevalence of urinary schistosomiasis in women of 75.6% (95% confidence limit 69.3 to 81.9%) was compared with a neighbouring village with similar socio-economic characteristics and a prevalence of 5.0% (95% confidence limit 0 to 11.75%). the women were questioned in malagasy about obstetrical history and urogynecological symptoms. the ... | 1998 | 9623935 |
| clinical quiz. trichomoniasis. | 1998 | 9630049 | |
| clinical manifestations and diagnosis of bacterial vaginosis in a clinic of sexually transmitted diseases. | the aim of the present study was to evaluate the prevalence of bacterial vaginosis (bv) and correlate it with the data from the patient history and clinical manifestations in women attending an std clinic and to compare two methods of diagnosis. | 1998 | 9630766 |
| clinical algorithms for the screening of pregnant women for stds in libreville, gabon: which alternatives? | sexually transmitted diseases (stds) remain one of the major public health problems in the developing world. to implement a systematic screening of stds among pregnant women in libreville, gabon, a preliminary cross sectional study on std prevalence and risk factors was performed in antenatal clinics. a score, integrating risk factors and elementary clinical signs for the screening of stds, showed higher performances compared with hierarchical algorithms. the prospective validation of this score ... | 1998 | 9634299 |
| community based study of sexually transmitted diseases in rural women in the highlands of papua new guinea: prevalence and risk factors. | to estimate the prevalence of sexually transmitted diseases (stds) and determine their risk factors/markers among a rural population of women in the highlands of papua new guinea. | 1998 | 9634324 |
| the tampon test for trichomoniasis: a comparison between conventional methods and a polymerase chain reaction for trichomonas vaginalis in women. | trichomonas vaginalis is the most common std worldwide and the infection has been linked with an increased risk of hiv transmission. we present a detailed comparison between conventional collection and testing methods and the polymerase chain reaction (pcr) tampon test for t vaginalis. | 1998 | 9634327 |
| trichomonas vaginalis and tritrichomonas foetus: expression of chitin at the cell surface. | the expression of chitin as a structural component of trichomonas vaginalis and tritrichomonas foetus was demonstrated by using enzymatic hydrolysis by recombinant (rec-) chitinase, chemical analysis, lectin, fluorescent calcofluor and antibody binding, glycosidases of known specificity, high-performance liquid chromatography (hplc), and flow cytometry. chitinous structures were characterized by their insolubility in hot alkali and by releasing glucosamine on hydrolysis with 6 n hcl. n,n'-diacet ... | 1998 | 9635443 |
| trichomonas vaginalis: in vitro phagocytosis of lactobacilli, vaginal epithelial cells, leukocytes, and erythrocytes. | this paper explores the interaction of two strains of trichomonas vaginalis, of high and low virulence, with the cell types present in the microenvironment of the parasite during human infections. with the use of transmission and scanning electron microscopy the sequence of internalization by t. vaginalis of döderlein's lactobacilli, and of vaginal epithelial cells, leukocytes, and erythrocytes was documented. furthermore, the degradation of ingested material by colocalization of acid phosphatas ... | 1998 | 9635448 |
| anaerobic bacteria in men with urethritis. | investigation of the urethral flora in men with urethritis, with particular reference to anaerobic bacteria. | 1998 | 9643328 |
| characterization of trichomonas vaginalis virus proteins in the pathogenic protozoan t. vaginalis. | the 4.6-kb double-stranded (ds) rna of trichomonas vaginalis virus (tvv)-t1 has been shown to encode two overlapping genes, cap and pol. in this study, a serum for specifically detecting viral cap gene product was raised against a recombinant protein, and sera for specifically detecting pol gene product were raised against synthetic oligopeptides. a 75-kda major protein and a 160-kda minor protein were detected by anti-cap serum in a tvv-t1 sample, indicating that the 75-kda protein is the viral ... | 1998 | 9645201 |
| axenic cultivation of trichomonas vaginalis in a serum-free medium. | mammalian serum or bovine serum albumin are essential for trichomonas vaginalis cultivated under axenic conditions. unfortunately, these components inhibit several biological properties of these parasites. pacsr is a serum replacement, free of bovine serum albumin. used for axenic cultivation of entamoeba histolytica. we show that pacsr is also useful for axenic cultivation of t. vaginalis. tubes containing 5.5 ml pehp, or tyi basal media, plus 8% pacsr (v/v), were inoculated with 10(3) trichomo ... | 1998 | 9645876 |
| rates of and risk factors for trichomoniasis among pregnant inmates in new york city. | trichomonas vaginalis is a common pathogen that is associated with adverse pregnancy outcomes and may serve as a cofactor in human immunodeficiency virus (hiv) transmission. | 1998 | 9662764 |
| detection of chlamydia trachomatis and trichomonas vaginalis in the vaginal introitus, posterior vagina, and endocervix by polymerase chain reaction. | 1998 | 9664518 | |
| the possible role of anaerobic bacteria in chronic prostatitis. | prostatis, the most common urological disease in men, afflicts between 25 and 50% of all adult men. four clinical categories are recognized: acute and chronic bacterial prostatitis, non-bacterial prostatitis and prostatodynia. the role of gram-positive aerobic bacteria and the different anaerobes in chronic bacterial prostatitis is still a matter of debate. during this study, the urethral discharge and the prostatic fluid obtained after prostatic massage of 50 patients with chronic prostatitis, ... | 1998 | 9669200 |
| comparison of tampon and urine as self-administered methods of specimen collection in the detection of chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis in women. | self-administered sampling techniques for the detection of sexually transmitted diseases (stds) are particularly useful due to their ease of collection and better patient compliance. urine specimens, and recently tampons, have been described as methods of specimen collection for the detection of some stds in women. in this study, 660 women had both first-void urine (fvu) and tampon specimens analysed by polymerase chain reaction (pcr) for the detection of chlamydia trachomatis, neisseria gonorrh ... | 1998 | 9671249 |
| chlamydia trachomatis: probable cause of prostatitis. | seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ... | 1998 | 9671250 |
| audit of diagnostic criteria for trichomonas vaginalis in a genitourinary medicine clinic. | a comparison between high vaginal swab (hvs) wet prep examination within the genitourinary medicine (gum) clinic and that made from an hvs transported in amies charcoal medium to the laboratory for the diagnosis of trichomonas vaginalis was made in a prospective study. clinic wet prep compared with amies had a sensitivity of 68% and specificity of nearly 100%. using the clinic wet prep alone, 9/30 (30%) cases would have been missed. | 1998 | 9671254 |
| purification and partial characterization of glyceraldehyde-phosphate dehydrogenase from electric organ of electrophorus electricus (l.). | the glyceraldehyde-phosphate dehydrogenase (gapdh, ec 1.2.1.12) was purified to homogeneity from electric organ of electrophorus electricus (l.) by a hydrophobic chromatography method on deacetylcolchicine-sepharose. the purification resulted in a 162 fold increase in specific activity of the gapdh and final yield was approximately 37%. the purified enzyme showed a single band in sds-page, with an apparent molecular mass of 36 kda. the purity of the colchicine-sepharose isolated material was ana ... | 1998 | 9679332 |
| cytologic identification of trichomonas vaginalis in urine from a male with long-standing sterile pyuria. | 1998 | 9684611 | |
| trichomonas vaginalis thymidine kinase: purification, characterization and search for inhibitors. | we report that a thymidine kinase (tk) activity is present in trichomonas vaginalis and can be separated from the deoxyribonucleoside phosphotransferase. t. vaginalis tk, purified 11200-fold to apparent homogeneity, has a molecular mass of 31500 da. it phosphorylates not only thymidine (km 0.18 microm) but also deoxycytidine (km 0.88 microm) and deoxyuridine (km 0.14 microm). in contrast with t. vaginalis deoxyribonucleoside phosphotransferase, the tk activity is strongly inhibited by novel deox ... | 1998 | 9693096 |
| correlation between aerobic and anaerobic resistance to metronidazole in trichomonads: application of a new computer program for permutation tests. | an indispensable step of any comparative study is testing the concordance between the distribution of phenetic traits and the evolutionary history of the taxa under study. here we present a computer program treept which can perform these tasks on the basis of a permutation test. the use of the program was demonstrated on trichomonad drug sensitivity data. the program can also perform permutation tests analogous to the t-test, analysis of variance (anova) and correlation analysis and is available ... | 1998 | 9694378 |
| phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. | over 90% of the open reading frame of gap genes for glycolytic glyceraldehyde-3-phosphate dehydrogenase (gapdh; ec 1.2.1.12) was obtained with pcr from five species of parabasala. with gap1 from trichomonas vaginalis obtained earlier, the data include two sequences each for three species. all sequences were colinear with t. vaginalis gap1 and shared with it as a synapomorphy a 10- to 11-residue insertion not found in any other gap and an s-loop with characteristic features of eubacterial gapdh. ... | 1998 | 9694668 |
| analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (bcecf) by hydrogenosomes in trichomonas vaginalis. | the fluorescent dye 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (bcecf) has been widely used as an indicator of cytosolic ph. here we report that bcecf localizes to hydrogenosomes (hydrogen-generating organelles found in several phylogenetically separate groups of anaerobic protists) in trichomonas vaginalis, where it was observable by fluorescence microscopy. its cellular location was confirmed by treatment of bcecf-loaded cells with diaminobenzidine and hydrogen peroxide together wi ... | 1998 | 9696354 |
| prevalence and effects of trichomoniasis in pregnancy. | 1998 | 9707712 | |
| testing promoter activity in the trypanosome genome: isolation of a metacyclic-type vsg promoter, and unexpected insights into rna polymerase ii transcription. | in trypanosomes, most genes are arranged in polycistronic transcription units. individual mrnas are generated by 5'-trans splicing and 3' polyadenylation. remarkably, no regulation of rna polymerase ii transcription has been detected although many rnas are differentially expressed during kinetoplastid life cycles. demonstration of specific class ii promoters is complicated by the difficulty in distinguishing between genuine promoter activity and stimulation of trans splicing. using vectors that ... | 1998 | 9709032 |
| sexually transmitted infections among sex workers in kwazulu-natal, south africa. | the purpose of this study was to investigate the prevalence of sexually transmitted infections, including human immunodeficiency virus (hiv), among female sex workers operating at truckstops in the kwazulu-natal midlands of south africa. | 1998 | 9713913 |
| a clinicobacteriological study on leucorrhoea. | the pathogens like trichomonas vaginalis (4.5%), n gonorrhoeae (2.7%) and c albicans (6.7%) were exclusively present in leucorrhoea. the other potential agents with their respective percentages in normal women and cases of leucorrhoea were u urealyticum (21.2% and 50.2%), actinomyces (29.7% and 41.6%), chlamydia trachomatis (17% and 48.8%), candida-like organisms (clo) (1.2% and 9.5%) and non-group b streptococci (4.2% and 16.7%). the percentages of urethral syndrome (65.8%), vaginal irritation ... | 1998 | 9715025 |
| cytokine and antibody responses in women infected with neisseria gonorrhoeae: effects of concomitant infections. | the levels of interleukin (il)-1, il-6, il-8, il-10, and transforming growth factor-beta in sera and genital tract secretions from women with gonococcal cervicitis and other genital infections were examined. cytokines were not elevated in genital secretions from gonococcus-infected compared with uninfected patients. the level of serum il-6 was higher in gonococcus-infected than in uninfected patients at recruitment. serum, but not local, il-1 and il-6 levels were elevated in patients concomitant ... | 1998 | 9728543 |
| cysteine proteases of trichomonas vaginalis degrade secretory leukocyte protease inhibitor. | sexually transmitted diseases, including trichomoniasis, are risk factors for acquisition of human immunodeficiency virus (hiv) infection. enhancement mechanisms are unknown. secretory leukocyte protease inhibitor (slpi) from saliva appears to prevent transmission of hiv through inhibition of virus entry into monocytic cells in vitro. this study was undertaken to determine if secreted cysteine proteases of trichomonas vaginalis degrade slpi and render it nonfunctional. it was determined if slpi ... | 1998 | 9728551 |
| evidence of independent gene duplications during the evolution of archaeal and eukaryotic family b dna polymerases. | eukaryotes and archaea both possess multiple genes coding for family b dna polymerases. in animals and fungi, three family b dna polymerases, alpha, delta, and epsilon, are responsible for replication of nuclear dna. we used a pcr-based approach to amplify and sequence phylogenetically conserved regions of these three dna polymerases from giardia intestinalis and trichomonas vaginalis, representatives of early-diverging eukaryotic lineages. phylogenetic analysis of eukaryotic and archaeal paralo ... | 1998 | 9729885 |