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effect of tween 40 and dtsr1 on l-arginine overproduction in corynebacterium crenatum.l-glutamate is an important precursor in the l-arginine (l-arg) biosynthetic pathway. various methods, including polyoxyethylene sorbitan monopalmitate (tween 40) addition and dtsr1 disruption, have been widely used to induce l-glutamate overproduction in corynebacterium glutamicum. in this study, a novel strategy for l-arg overproduction through tween 40 trigger and δdtsr1 mutant were proposed in corynebacterium crenatum.201526264811
improved production of poly(lactic acid)-like polyester based on metabolite analysis to address the rate-limiting step.the biosynthesis of poly(lactic acid) (pla)-like polymers, composed of >99 mol% lactate and a trace amount of 3-hydroxybutyrate, in engineered corynebacterium glutamicum consists of two steps; the generation of the monomer substrate lactyl-coenzyme a (coa) and the polyhydroxyalkanoate (pha) synthase-catalyzed polymerization of lactyl-coa. in order to increase polymer productivity, we explored the rate-limiting step in pla-like polymer synthesis based on quantitative metabolite analysis using liq ...201426267112
l-citrulline production by metabolically engineered corynebacterium glutamicum from glucose and alternative carbon sources.l-citrulline plays an important role in human health and nutrition and is an intermediate of the l-arginine biosynthetic pathway. l-citrulline is a by-product of l-arginine production by corynebacterium glutamicum. in this study, c. glutamicum was engineered for overproduction of l-citrulline as major product without l-arginine being produced as by-product. to this end, l-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argr and conversion of l-citrulline towards ...201426267114
anaerobic growth of corynebacterium glutamicum via mixed-acid fermentation.corynebacterium glutamicum, a model organism in microbial biotechnology, is known to metabolize glucose under oxygen-deprived conditions to l-lactate, succinate, and acetate without significant growth. this property is exploited for efficient production of lactate and succinate. our detailed analysis revealed that marginal growth takes place under anaerobic conditions with glucose, fructose, sucrose, or ribose as a carbon and energy source but not with gluconate, pyruvate, lactate, propionate, o ...201526276118
production of carbon-13-labeled cadaverine by engineered corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate.methanol, a one-carbon compound, can be utilized by a variety of bacteria and other organisms as carbon and energy source and is regarded as a promising substrate for biotechnological production. in this study, a strain of non-methylotrophic corynebacterium glutamicum, which was able to produce the polyamide building block cadaverine as non-native product, was engineered for co-utilization of methanol. expression of the gene encoding nad+-dependent methanol dehydrogenase (mdh) from the natural m ...201526276544
transcription of malp is subject to phosphotransferase system-dependent regulation in corynebacterium glutamicum.the gram-positive corynebacterium glutamicum co-metabolizes most carbon sources such as the phosphotransferase system (pts) sugar glucose and the non-pts sugar maltose. maltose is taken up via the abc-transporter musefgk2i, and is further metabolized to glucose phosphate by amylomaltase malq, maltodextrin phosphorylase malp, glucokinase glk and phosophoglucomutase pgm. surprisingly, growth of c. glutamicum strains lacking the general pts components ei or hpr was strongly impaired on the non-pts ...201526296766
engineering a lysine-on riboswitch for metabolic control of lysine production in corynebacterium glutamicum.riboswitches are natural rna elements that regulate gene expression by binding a ligand. here, we demonstrate the possibility of altering a natural lysine-off riboswitch from eschericia coli (ecrs) to a synthetic lysine-on riboswitch and using it for metabolic control. to this end, a lysine-on riboswitch library was constructed using teta-based dual genetic selection. after screening the library, the functionality of the selected lysine-on riboswitches was examined using a report gene, lacz. sel ...201526300047
application of granular activated carbon/mnfe₂o₄ composite immobilized on c. glutamicum mtcc 2745 to remove as(iii) and as(v): kinetic, mechanistic and thermodynamic studies.the main objective of the present study was to investigate the efficiency of corynebacterium glutamicum mtcc 2745 immobilized on granular activated carbon/mnfe2o4 (gac/mnfe2o4) composite to treat high concentration of arsenic bearing wastewater. non-linear regression analysis was done for determining the best-fit kinetic model on the basis of three correlation coefficients and three error functions and also for predicting the parameters involved in kinetic models. the results showed that fractal ...201626322840
dynamic transmission of protein allostery without structural change: spatial pathways or global modes?we examine the contrast between mechanisms for allosteric signaling that involve structural change, and those that do not, from the perspective of allosteric pathways. in particular we treat in detail the case of fluctuation-allostery by which amplitude modulation of the thermal fluctuations of the elastic normal modes conveys the allosteric signal, and address the question of what an allosteric pathway means in this case. we find that a perturbation theory of thermal elastic solids and nonpertu ...201526338443
single-domain peptidyl-prolyl cis/trans isomerase fkpa from corynebacterium glutamicum improves the biomass yield at increased growth temperatures.peptidyl-prolyl cis/trans isomerases (ppiases) catalyze the rate-limiting protein folding step at peptidyl bonds preceding proline residues and were found to be involved in several biological processes, including gene expression, signal transduction, and protein secretion. representative enzymes were found in almost all sequenced genomes, including corynebacterium glutamicum, a facultative anaerobic gram-positive and industrial workhorse for the production of amino acids. in c. glutamicum, a pre ...201526341203
spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform.cell-to-cell heterogeneity typically evolves due to a manifold of biological and environmental factors and special phenotypes are often relevant for the fate of the whole population but challenging to detect during conventional analysis. we demonstrate a microfluidic single-cell cultivation platform that incorporates several hundred growth chambers, in which isogenic bacteria microcolonies growing in cell monolayers are tracked by automated time-lapse microscopy with spatiotemporal resolution. t ...201526348020
improving the electro-transformation efficiency of corynebacterium glutamicum by weakening its cell wall and increasing the cytoplasmic membrane fluidity.to improve the transformation efficiency of corynebacterium glutamicum cells with heterogenous plasmid dna and single-strand dna (ssdna) using a methodology based on electro-transformation.201526354854
engineering of corynebacterium glutamicum for growth and succinate production from levoglucosan, a pyrolytic sugar substrate.thermochemical processing provides continuous production of bio-oils from lignocellulosic biomass. levoglucosan, a pyrolytic sugar substrate c6h10o5 in a bio-oil, has been used for ethanol production using engineered escherichia coli. here we provide the first example for succinate production from levoglucosan with corynebacterium glutamicum, a well-known industrial amino acid producer. heterologous expression of a gene encoding a sugar kinase from lipomyces starkeyi, gibberella zeae or pseudomo ...201526363018
cutting the gordian knot: identifiability of anaplerotic reactions in corynebacterium glutamicum by means of (13) c-metabolic flux analysis.corynebacterium glutamicum is the major workhorse for the microbial production of several amino and organic acids. as long as these derive from tricarboxylic acid cycle intermediates, the activity of anaplerotic reactions is pivotal for a high biosynthetic yield. to determine single anaplerotic activities (13) c-metabolic flux analysis ((13) c-mfa) has been extensively used for c. glutamicum, however with different network topologies, inconsistent or poorly determined anaplerotic reaction rates. ...201626375179
molecular characterization of a eukaryotic-like phenol hydroxylase from corynebacterium glutamicum.this study focuses on the genetic and biochemical characterization of phenol hydroxylase (phe, ncgl2588) from corynebacterium glutamicum that shares 31% identity in amino acids with phenol hydroxylase from yeast trichosporon cutaneum but less similarity with that from bacteria. the phe deletion mutant significantly reduced its ability to grow with phenol as the sole carbon and energy source. expression of the phe gene was strongly induced with phenol and also subject to the control of carbon cat ...201526377129
metabolic engineering of the mixed-acid fermentation pathway of escherichia coli for anaerobic production of glutamate and itaconate.itaconic acid, an unsaturated c5-dicarboxylic acid, is a biobased building block for the polymer industry. the purpose of this study was to establish proof of principle for an anaerobic fermentation process for the production of itaconic acid by modification of the mixed acid fermentation pathway of e. coli. e. coli bw25113 (de3) and the phosphate acetyltransferase (pta) and lactate dehydrogenase (ldha) deficient strain e. coli bw25113 (de3) δpta-δldha were used to study anaerobic itaconate prod ...201526384341
reconstruction and analysis of a genome-scale metabolic network of corynebacterium glutamicum s9114.corynebacterium glutamicum s9114 is commonly used for industrial glutamate production. therefore, a comprehensive understanding of the physiological and metabolic characteristics of c. glutamicum is important for developing its potential for industrial production. a genome-scale metabolic model, ijm658, was reconstructed based on genome annotation and literature mining. the model consists of 658 genes, 984 metabolites and 1065 reactions. the model quantitatively predicted c. glutamicum growth on ...201626392034
production of protocatechuic acid by corynebacterium glutamicum expressing chorismate-pyruvate lyase from escherichia coli.protocatechuic acid (3,4-dihydroxybenzoic acid; pca) serves as a building block for polymers and pharmaceuticals. in this study, the biosynthetic pathway for pca from glucose was engineered in corynebacterium glutamicum. the pathway to pca-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (cpl) and 4-hydroxybenzoate hydroxylase (4-hba hydroxylase). as c. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-hba hyd ...201626392137
modular pathway engineering of corynebacterium glutamicum for production of the glutamate-derived compounds ornithine, proline, putrescine, citrulline, and arginine.the glutamate-derived bioproducts ornithine, citrulline, proline, putrescine, and arginine have applications in the food and feed, cosmetic, pharmaceutical, and chemical industries. corynebacterium glutamicum is not only an excellent producer of glutamate but also of glutamate-derived products. here, engineering targets beneficial for ornithine production were identified and the advantage of rationally constructing a platform strain for the production of the amino acids citrulline, proline, and ...201526393954
3-amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant corynebacterium glutamicum.the production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-ahba) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. recombinant corynebacterium glutamicum strain kt01 expressing grih and grii derived from streptomyces griseus produced 3,4-ahba from the sweet sorghum juice of cultivar sil-05 at a final concentration (1.0 ...201526409852
arar, an l-arabinose-responsive transcriptional regulator in corynebacterium glutamicum atcc 31831, exerts different degrees of repression depending on the location of its binding sites within the three target promoter regions.in corynebacterium glutamicum atcc 31831, a laci-type transcriptional regulator arar, represses the expression of l-arabinose catabolism (arabda), uptake (arae), and the regulator (arar) genes clustered on the chromosome. arar binds to three sites: one (bsb) between the divergent operons (arabda and galm-arar) and two (bse1 and bse2) upstream of arae. l-arabinose acts as an inducer of the arar-mediated regulation. here, we examined the roles of these arar-binding sites in the expression of the a ...201526416832
involvement of the osrr gene in the hydrogen peroxide-mediated stress response of corynebacterium glutamicum.a transcriptional profile of the h2o2-adapted corynebacterium glutamicum ha strain reveals a list of upregulated regulatory genes. among them, we selected orf ncgl2298, designated osrr and analyzed its role in h2o2 adaptation. the osrr-deleted (δosrr) mutant had defective growth in minimal medium, which was even more pronounced in an osrr deletion mutant of an ha strain. the δosrr strain displayed increased sensitivity to h2o2. in addition to h2o2 sensitivity, the δosrr strain was found to be te ...201626433092
metabolic engineering of a laboratory-evolved thermobifida fusca muc strain for malic acid production on cellulose and minimal treated lignocellulosic biomass.malic acid is mainly used as an acidulant and taste enhancer in the beverage and food industry. previously, a mutant strain thermobifida fusca muc, obtained by adaptive evolution was found to accumulate malic acid on cellulose with low yield. in this study, the malic acid synthesis pathway in t. fusca muc was confirmed to be from phosphoenolpyruvate to oxaloacetate, followed by reduction of oxaloacetate to malate. to increase the yield of malic acid by the muc strain significantly, the carbon fl ...201726439318
5-aminolevulinic acid production in engineered corynebacterium glutamicum via c5 biosynthesis pathway.ala (5-aminolevulinic acid) is an important intermediate in the synthesis of tetrapyrroles and the use of ala has been gradually increasing in many fields, including medicine and agriculture. in this study, improved biological production of ala in corynebacterium glutamicum was achieved by overexpressing glutamate-initiated c5 pathway. for this purpose, copies of the glutamyl t-rna reductase hema from several bacteria were mutated by site-directed mutagenesis of which a hema version from salmone ...201526453466
biosensor-driven adaptive laboratory evolution of l-valine production in corynebacterium glutamicum.adaptive laboratory evolution has proven a valuable strategy for metabolic engineering. here, we established an experimental evolution approach for improving microbial metabolite production by imposing an artificial selective pressure on the fluorescent output of a biosensor using fluorescence-activated cell sorting. cells showing the highest fluorescent output were iteratively isolated and (re-)cultivated. the l-valine producer corynebacterium glutamicum δacee was equipped with an l-valine-resp ...201526453945
breeding l-arginine-producing strains by a novel mutagenesis method: atmospheric and room temperature plasma (artp).a plasma jet, driven by an active helium atom supplied with an atmospheric and room temperature plasma (artp) biological breeding system, was used as a novel method to breed l-arginine high-yielding strains. a mutant with resistance to l-homoarginine and 8-azaguaine, arg 3-15 (l-ha(r), 8-ag(r), l-his(-)), was screened after several rounds of screening. the l-arginine production of these mutants was more than that of the original strain, increased by 43.79% for arg 3-15. moreover, n-acetyl-l-glut ...201626460578
identification of two mutations increasing the methanol tolerance of corynebacterium glutamicum.methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes.201526474849
caenorhabditis elegans star formation and negative chemotaxis induced by infection with corynebacteria.caenorhabditis elegans is one of the major model systems in biology based on advantageous properties such as short life span, transparency, genetic tractability and ease of culture using an escherichia coli diet. in its natural habitat, compost and rotting plant material, this nematode lives on bacteria. however, c. elegans is a predator of bacteria, but can also be infected by nematopathogenic coryneform bacteria such microbacterium and leucobacter species, which display intriguing and diverse ...201626490043
the impact of the c-terminal domain on the gating properties of msccg from corynebacterium glutamicum.the mechanosensitive (ms) channel msccg from the soil bacterium corynebacterium glutamicum functions as a major glutamate exporter. msccg belongs to a subfamily of the bacterial mscs-like channels, which play an important role in osmoregulation. to understand the structural and functional features of msccg, we investigated the role of the carboxyl-terminal domain, whose relevance for the channel gating has been unknown. the chimeric channel mscs-(c-msccg), which is a fusion protein between the c ...201626494188
regulons of global transcription factors in corynebacterium glutamicum.corynebacterium glutamicum, a high gc content gram-positive soil bacterium in actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. since its genome sequence was first published, its versatile metabolic pathways and their genetic components and regulatory mechanisms have been extensively studied. previous studies on transcriptional factors, including two-component systems and σ facto ...201626496920
construction of a novel twin-arginine translocation (tat)-dependent type expression vector for secretory production of heterologous proteins in corynebacterium glutamicum.corynebacterium glutamicum is recognized as a favorable host for the secretory production of heterologous proteins. however, there are few secretion-type expression vectors available for protein production in c. glutamicum. in this study, we constructed a shuttle expression vector pau3, which harbors the strong promoter tac-m for constitutive gene transcription, the consensus rbs sequence for protein translation, and the strong cgr_0949 signal sequence for protein secretion via the tat pathway i ...201526499464
structural insight into dihydrodipicolinate reductase from corybebacterium glutamicum for lysine biosynthesis.dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an nad(p)h cofactor in l-lysine biosynthesis. to increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from corynebacterium glutamicum (cgdapb). cgdapb functions as a tetramer, and each protomer is composed of two domains, an nterminal domain and a c-terminal domain. the n-terminal domain mainly ...201626502738
engineering corynebacterium glutamicum for the production of 2,3-butanediol.2,3-butanediol is an important bulk chemical with a wide range of applications. in bacteria, this metabolite is synthesised from pyruvate via a three-step pathway involving α-acetolactate synthase, α-acetolactate decarboxylase and 2,3-butanediol dehydrogenase. thus far, the best producers of 2,3-butanediol are pathogenic strains, hence, the development of more suitable organisms for industrial scale fermentation is needed. herein, 2,3-butanediol production was engineered in the generally regarde ...201526511723
monomeric corynebacterium glutamicum n-acetyl glutamate kinase maintains sensitivity to l-arginine but has a lower intrinsic catalytic activity.n-acetyl glutamate kinase (nagk) is a key enzyme in the synthesis of l-arginine, and l-arginine-sensitive nagk typically has hexameric architecture. defining the relationship between this architecture and l-arginine inhibition can provide a foundation to identify the key amino acids involved in the allosteric regulation network of l-arginine. in the present study, the key amino acids in the n-terminal helix (n-helix) of corynebacterium glutamicum (cg) nagk required for hexamer formation were det ...201626512006
non-invasive microbial metabolic activity sensing at single cell level by perfusion of calcein acetoxymethyl ester.phase contrast microscopy cannot give sufficient information on bacterial metabolic activity, or if a cell is dead, it has the fate to die or it is in a viable but non-growing state. thus, a reliable sensing of the metabolic activity helps to distinguish different categories of viability. we present a non-invasive instantaneous sensing method using a fluorogenic substrate for online monitoring of esterase activity and calcein efflux changes in growing wild type bacteria. the fluorescent conversi ...201526513257
engineering the glycolytic pathway: a potential approach for improvement of biocatalyst performance.the glycolytic pathway is a main driving force in the fermentation process as it produces energy, cell component precursors, and fermentation products. given its importance, the glycolytic pathway can be considered as an attractive target for the metabolic engineering of industrial microorganisms. however, many attempts to enhance glycolytic flux, by overexpressing homologous or heterologous genes encoding glycolytic enzymes, have been unsuccessful. in contrast, significant enhancement in glycol ...201526513591
high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum.enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ...201526519562
corynebacterium glutamicum ggtb encodes a functional γ-glutamyl transpeptidase with γ-glutamyl dipeptide synthetic and hydrolytic activity.in this work the role of γ-glutamyl transpeptidase in the metabolism of γ-glutamyl dipeptides produced by corynebacterium glutamicum atcc 13032 was studied. the enzyme is encoded by the gene ggtb (cg1090) and synthesized as a 657 amino acids long preprotein. gamma-glutamyl transpeptidase activity was found to be associated with intact cells of c. glutamicum and was abolished upon deletion of ggtb. bioinformatic analysis indicated that the enzyme is a lipoprotein and is attached to the outer side ...201626528625
fudc, a protein primarily responsible for furfural detoxification in corynebacterium glutamicum.lignocellulosic hydrolysates contain compounds that inhibit microbial growth and fermentation, thereby decreasing the productivity of biofuel and biochemical production. in particular, the heterocyclic aldehyde furfural is one of the most toxic compounds found in these hydrolysates. we previously demonstrated that corynebacterium glutamicum converts furfural into the less toxic compounds furfuryl alcohol and 2-furoic acid. to date, however, the genes involved in these oxidation and reduction rea ...201626541332
metabolic engineering of corynebacterium glutamicum for the de novo production of ethylene glycol from glucose.development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. ethylene glycol (eg) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical route. herein, we report a novel biosynthetic route to produce eg from glucose by the extension of serine synthesis pathway of corynebacterium glutamicum. the eg synthesis is achie ...201626556130
engineering corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose.corynebacterium glutamicum is generally regarded as a safe microorganism and is used to produce many biochemicals, including l-glutamate. 5-aminolevulinic acid (ala) is an l-glutamate derived non-protein amino acid, and is widely applied in fields such as medicine and agriculture.201526577071
crystal structure and biochemical characterization of tetrahydrodipicolinate n-succinyltransferase from corynebacterium glutamicum.tetrahydrodipicolinate n-succinyltransferase (dapd) is an enzyme involved in the biosynthesis of l-lysine by converting tetrahydrodipicolinate into n-succinyl-l-2-amino-6-oxopimelate, using succinyl-coa as a cofactor. we determined the crystal structure of dapd from corynebacterium glutamicum (cgdapd). cgdapd functions as a trimer, and each monomer consists of three domains: an n-terminal helical domain (ntd), a left-handed β-helix (lβh) domain, and a β c-terminal domain (ctd). the mode of cofac ...201526602189
enhanced production of 3-hydroxypropionic acid from glucose via malonyl-coa pathway by engineered escherichia coli.in this study, production of 3-hp via malonyl-coa was investigated by using metabolically engineered escherichia coli carrying heterogeneous acetyl-coa carboxylase (acc) from corynebacterium glutamicum and codon-optimized malonyl-coa reductase (mcr) from chloroflexus aurantiacus. three engineered e. coli strains with different host-vector systems were constructed and investigated. the results indicated that the combination of e. coli bl21(de3) and pet28a was the most efficient host-vector system ...201626606325
identification of the phd gene cluster responsible for phenylpropanoid utilization in corynebacterium glutamicum.phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. we found that the biotechnologically important soil bacterium corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-hydroxyphenyl)propionic acid as sole carbon and energy sources. global gene expression analyses identified a gene cluster (cg0340-cg0341 and cg0344-cg0347), which showed increased tr ...201626610800
metabolic engineering of corynebacterium glutamicum for efficient production of 5-aminolevulinic acid.5-aminolevulinic acid (5-ala) has recently attracted attention for its potential applications in the fields of medicine and agriculture. in this study, corynebacterium glutamicum was firstly engineered for 5-ala production via the c4 pathway. hema encoding 5-aminolevulinic acid synthase from rhodobacter sphaeroides was codon optimized and expressed in c. glutamicum atcc13032, resulting in accumulation of 5-ala. deletion of all known genes responsible for the formation of acetate and lactate furt ...201626616115
metabolic engineering corynebacterium glutamicum to produce triacylglycerols.in this study, we metabolically engineered corynebacterium glutamicum to produce triacylglycerols (tags) by completing and constraining a de novo tag biosynthesis pathway. first, the plasmid pz8_tag4 was constructed which allows the heterologous expression of four genes: three (atf1 and atf2, encoding the diacylglycerol acyltransferase; pgpb, encoding the phosphatidic acid phosphatase) to complete the tag biosynthesis pathway, and one gene (tada) for lipid body assembly. second, we applied four ...201626645801
altered acetylation and succinylation profiles in corynebacterium glutamicum in response to conditions inducing glutamate overproduction.the bacterium corynebacterium glutamicum is utilized during industrial fermentation to produce amino acids such as l-glutamate. during l-glutamate fermentation, c. glutamicum changes the flux of central carbon metabolism to favor l-glutamate production, but the molecular mechanisms that explain these flux changes remain largely unknown. here, we found that the profiles of two major lysine acyl modifications were significantly altered upon glutamate overproduction in c. glutamicum; acetylation de ...201626663479
in vitro functional characterization of the na+/h+ antiporters in corynebacterium glutamicum.corynebacterium glutamicum, typically used as industrial workhorse for amino acid production, is a moderately salt-alkali-tolerant microorganism with optimal growth at ph 7-9. however, little is known about the mechanisms of salt-alkali tolerance in c. glutamicum. here, the catalytic capacity of three putative na(+)/h(+) antiporters from c. glutamicum (designated as cg-mrp1, cg-mrp2 and cg-nhap) were characterized in an antiporter-deficient escherichia coli knabc strain. only cg-mrp1 was able to ...201626667218
production of orotic acid by a klura3δ mutant of kluyveromyces lactis.we demonstrated that a klura3δ, mutant of the yeast kluyveromyces lactis is able to produce and secrete into the growth medium considerable amounts of orotic acid. using yeast extract-peptone-glucose (ypd) based media we optimized production conditions in flask and bioreactor cultures. with cells grown in ypd 5% glucose medium, the best production in flask was obtained with a 1:12.5 ratio for flask: culture volume, 180 rpm, 28°c and 200 mm mops for ph stabilization at neutral values (initial cul ...201626707627
high-titer biosynthesis of hyaluronic acid by recombinant corynebacterium glutamicum.hyaluronic acid (ha) plays important roles in human tissue system, thus it is highly desirable for various applications, such as in medical, clinic and cosmetic fields. the wild microbial producer of ha, streptococcus, was restricted by its potential pathogens, hence different recombinant hosts are being explored. in this work, we engineered corynebacterium glutamicum, a gras (generally recognized as safe) organism free of exotoxins and endotoxins to produce ha with high titer and satisfied mw . ...201626709615
rnase iii mediated cleavage of the coding region of mraz mrna is required for efficient cell division in corynebacterium glutamicum.the corynebacterium glutamicum r cgr_1959 gene encodes an endoribonuclease of the rnase iii family. deletion mutant of cgr_1959 (δrnc mutant) showed an elongated cell shape, and presence of several lines on the cell surface, indicating a required of rnase iii for maintaining normal cell morphology in c. glutamicum. the level of mraz mrna was increased, whereas cgr_1596 mrna encoding a putative cell wall hydrolase and ftsex mrna were decreased in the δrnc mutant. the half-life of mraz mrna was si ...201626713407
enhanced production of recombinant proteins with corynebacterium glutamicum by deletion of insertion sequences (is elements).in most bacteria, various jumping genetic elements including insertion sequences elements (is elements) cause a variety of genetic rearrangements resulting in harmful effects such as genome and recombinant plasmid instability. the genetic stability of a plasmid in a host is critical for high-level production of recombinant proteins, and in this regard, the development of an is element-free strain could be a useful strategy for the enhanced production of recombinant proteins. corynebacterium glut ...201526715464
structure of amtr, the global nitrogen regulator of corynebacterium glutamicum, in free and dna-bound forms.corynebacterium glutamicum is a bacterium used for industrial amino acid production, and understanding its metabolic pathway regulation is of high biotechnological interest. here, we report crystal structures of amtr, the global nitrogen regulator of c. glutamicum, in apo (2.25-å and 2.65-å resolution) and dna-bound (3-å resolution) forms. these structures reveal an all-α homodimeric tetr family regulator composed of a helix-turn-helix-hosting n-terminal dna-binding domain and a c-terminal dimer ...201626744254
[characterization of l-aspartate-α-decarboxylase from bacillus subtilis].as an important material in pharmaceutical and chemical industry, β-alanine was mainly produced by chemical methods. l-aspartate-α-decarboxylase could catalyze the α-decarboxylation from l-aspartate to β-alanine. determinations for specific activities of pands from escherichia coli, corynebacterium glutamicum and bacillus subtilis were performed in this study (0.98 u/mg, 7.52 u/mg and 8.4 u/mg respectively). the optimal temperature and ph of pands from c. glutamicum and b. subtilis were 65 degre ...201526762040
web application for genetic modification flux with database to estimate metabolic fluxes of genetic mutants.computational analysis of metabolic fluxes is essential in understanding the structure and function of a metabolic network and in rationally designing genetically modified mutants for an engineering purpose. we had presented the genetic modification flux (gmf) that predicts the flux distribution of a broad range of genetically modified mutants. to enhance the feasibility and usability of gmf, we have developed a web application with a metabolic network database to predict a flux distribution of ...201626777238
metabolic engineering of corynebacterium glutamicum atcc13032 to produce s-adenosyl-l-methionine.as an important biological methyl group donor, s-adenosyl-l-methionine is used as nutritional supplement or drug for various diseases, but bacterial strains that can efficiently produce s-adenosyl-l-methionine are not available. in this study, corynebacterium glutamicum strain hw104 which can accumulate s-adenosyl-l-methionine was constructed from c. glutamicum atcc13032 by deleting four genes thrb, metb, mcbr and ncgl2640, and six genes metk, vgb, lysc(m), hom(m), metx and mety were overexpress ...201626777246
development of a potential stationary-phase specific gene expression system by engineering of sigb-dependent cg3141 promoter in corynebacterium glutamicum.corynebacterium glutamicum is a non-pathogenic, non-sporulating gram-positive soil bacterium that has been used for the industrial production of various proteins and chemicals. to achieve enhanced and economical production of target molecules, the development of strong auto-inducible promoters is desired, which can be activated without expensive inducers and has significant advantages for industrial-scale use. here, we developed a stationary-phase gene expression system by engineering a sigma fa ...201626782746
complete genome sequence of corynebacterium glutamicum cp, a chinese l-leucine producing strain.here, we report the complete genome sequence of corynebacterium glutamicum cp, an industrial l-leucine producing strain in china. the whole genome consists of a circular chromosome and a plasmid. the comparative genomics analysis shows that there are many mutations in the key enzyme coding genes relevant to l-leucine biosynthesis compared to c. glutamicum atcc 13032.201626784991
the extracytoplasmic function σ factor σ(c) regulates expression of a branched quinol oxidation pathway in corynebacterium glutamicum.bacteria modify their expression of different terminal oxidases in response to oxygen availability. corynebacterium glutamicum, a facultative anaerobic bacterium of the phylum actinobacteria, possesses aa3 -type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced by oxygen limitation. we report that an extracytoplasmic function σ factor, σ(c) , is responsible for the regulation of this process. chromatin immunoprecipitation with microarray analysis detected ...201626789738
adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative corynebacterium glutamicum that co-utilizes cellobiose and xylose.an efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. the industrially important bacterium corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. however, c. glutamicum atcc 13032 is incapable of pts-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bg) and cellobiose-specific pts permeas ...201626801253
modular optimization of a hemicellulose-utilizing pathway in corynebacterium glutamicum for consolidated bioprocessing of hemicellulosic biomass.hemicellulose, which is the second most abundant polysaccharide in nature after cellulose, has the potential to become a major feedstock for microbial fermentation to produce various biofuels and chemicals. to utilize hemicellulose economically, it is necessary to develop a consolidated bioprocess (cbp), in which all processes from biomass degradation to the production of target products occur in a single bioreactor. here, we report a modularly engineered corynebacterium glutamicum strain suitab ...201626808593
characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation in corynebacterium glutamicum.4-cresol is not only a significant synthetic intermediate for production of many aromatic chemicals, but also a priority environmental pollutant because of its toxicity to higher organisms. in our previous studies, a gene cluster implicated to be involved in 4-cresol catabolism, crecdefghir, was identified in corynebacterium glutamicum and partially characterized in vivo. in this work, we report on the discovery of a novel 4-cresol biodegradation pathway that employs phosphorylated intermediates ...201626817843
corynebacterium glutamicum metabolic engineering with crispr interference (crispri).corynebacterium glutamicum is an important organism for the industrial production of amino acids. metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. to facilitate the mapping of gene expression levels to metabolic outputs, we applied crispr interference (crispri) technology using deactivated cas9 (dcas9) to repress genes in c. glutamicum. we then determined the effects of target repre ...201626829286
tatc-dependent translocation of pyoverdine is responsible for the microbial growth suppression.infections are often not caused by a colonization of pseudomonas aeruginosa alone but by a consortium of other bacteria. little is known about the impact of p. aeruginosa on the growth of other bacteria upon coinfection. here, cell-ree culture supernatants obtained from p. aeruginosa suppressed the growth of a number of bacterial strains such as corynebacterium glutamicum, bacillus subtilis, staphylococcus aureus, and agrobacterium tumefaciens, but had little effect on the growth of escherichia ...201626832668
strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression.with the availability of the whole genome sequence of escherichia coli or corynebacterium glutamicum, strategies for directed dna manipulation have developed rapidly. dna manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. dna manipulation involves modifying the autologous genes and expressing the heterogenous genes. two alternative approaches, using electroporation linear dna or recombinant suicide ...201626834010
the actinobacterium corynebacterium glutamicum, an industrial workhorse.starting as a glutamate producer, corynebacterium glutamicum has played a variety of roles in the industrial production of amino acids, one of the most important areas of white biotechnology. from shortly after its genome information became available, c. glutamicum has been applied in various production processes for value-added chemicals, fuels, and polymers, as a key organism in industrial biotechnology alongside the surprising progress in systems biology and metabolic engineering. in addition ...201626838341
optimization of large-ring cyclodextrin production from starch by amylomaltase from corynebacterium glutamicum and effect of organic solvent on product size.to increase yield of starch conversion to large-ring cyclodextrins (lr-cds) by amylomaltase from corynebacterium glutamicum (cgam).201626849173
mutagenesis for improvement of activity and thermostability of amylomaltase from corynebacterium glutamicum.this work aims to improve thermostability of amylomaltase from a mesophilic corynebacterium glutamicum (cgam) by random and site-directed mutagenesis. from error prone pcr, a mutated cgam with higher thermostability at 50 °c compared to the wild-type was selected and sequenced. the result showed that the mutant contains a single mutation of a406v. site-directed mutagenesis was then performed to construct a406v and a406l. both mutated cgams showed higher intermolecular transglucosylation activity ...201626875536
diverse effects of a biosurfactant from rhodococcus ruber iegm 231 on the adhesion of resting and growing bacteria to polystyrene.this study evaluated the effects of a trehalolipid biosurfactant produced by rhodococcus ruber iegm 231 on the bacterial adhesion and biofilm formation on the surface of polystyrene microplates. the adhesion of gram-positive (arthrobacter simplex, bacillus subtilis, brevibacterium linens, corynebacterium glutamicum, micrococcus luteus) and gram-negative (escherichia coli, pseudomonas fluorescencens) bacteria correlated differently with the cell hydrophobicity and surface charge. in particular, e ...201626888203
microfluidic screening of electric fields for electroporation.electroporation is commonly used to deliver molecules such as drugs, proteins, and/or dna into cells, but the mechanism remains poorly understood. in this work a rapid microfluidic assay was developed to determine the critical electric field threshold required for inducing bacterial electroporation. the microfluidic device was designed to have a bilaterally converging channel to amplify the electric field to magnitudes sufficient to induce electroporation. the bacterial cells are introduced into ...201626893024
a new strategy for production of 5-aminolevulinic acid in recombinant corynebacterium glutamicum with high yield.5-aminolevulinic acid (ala), a nonprotein amino acid involved in tetrapyrrole synthesis, has been widely applied in agriculture, medicine, and food production. many engineered metabolic pathways have been constructed; however, the production yields are still low. in this study, several 5-aminolevulinic acid synthases (alass) from different sources were evaluated and compared with respect to their ala production capacities in an engineered corynebacterium glutamicum cgs1 strain that can accumulat ...201626921424
enhancement of 1,5-diaminopentane production in a recombinant strain of corynebacterium glutamicum by tween 40 addition. 201626923131
efficient production of α-ketoglutarate in the gdh deleted corynebacterium glutamicum by novel double-phase ph and biotin control strategy.production of l-glutamate using a biotin-deficient strain of corynebacterium glutamicum has a long history. the process is achieved by controlling biotin at suboptimal dose in the initial fermentation medium, meanwhile feeding nh4oh to adjust ph so that α-ketoglutarate (α-kg) can be converted to l-glutamate. in this study, we deleted glutamate dehydrogenase (gdh1 and gdh2) of c. glutamicum gkg-047, an l-glutamate overproducing strain, to produce α-kg that is the direct precursor of l-glutamate. ...201626946492
3-methyl-1-butanol biosynthesis in an engineered corynebacterium glutamicum.biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. in the present work, 3-methyl-1-butanol production in engineered corynebacterium glutamicum was studied. α-ketoisovalerate decarboxylase gene (kivd) from lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adha, an ...201626961908
pathway construction and metabolic engineering for fermentative production of ectoine in escherichia coli.ectoine is a protective agent and stabilizer whose synthesis pathway exclusively exists in select moderate halophiles. a novel established process called "bacterial milking" efficiently synthesized ectoine in moderate halophiles, however, this method places high demands on equipment and is cost prohibitive. in this study, we constructed an ectoine producing strain by introducing the ectoine synthesis pathway into escherichia coli and improved its production capacity. firstly, the ectabc gene clu ...201626969253
engineering of corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with co.the possibilities to utilize one-carbon substrates (c1) like co, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. for the first time, methyl acetate (meoac), which can be formed from carbonylation of methanol with co, was demonstrated to be an alternative carbon source for the cell growth of corynebacterium glutamicum as a model microbial cell factory. to do so, a carboxyl esterase activity was necessary to hydrolyze meoac to methanol and acetate. al ...201626970052
pyruvate kinase deletion as an effective phenotype to enhance lysine production in corynebacterium glutamicum atcc13032: redirecting the carbon flow to a precursor metabolite.various attempts have been made to enhance lysine production in corynebacterium glutamicum. pyruvate kinase (pyk) defect is one of the strategies used to enhance the supply of oxaloacetic acid (oaa), a precursor metabolite for lysine biosynthesis. however, inconsistent effects of this mutation have been reported: positive effects of pyk defect in mutants having phosphoenolpyruvate carboxylase (pepc) desensitized to feedback inhibition by aspartic acid, while negative effects in simple pyk gene ( ...201626983943
production of succinic acid by metabolically engineered microorganisms.succinic acid (sa) has been recognized as one of the most important bio-based building block chemicals due to its numerous potential applications. for the economical bio-based production of sa, extensive research works have been performed on developing microbial strains by metabolic engineering as well as fermentation and downstream processes. here we review metabolic engineering strategies applied for bio-based production of sa using representative microorganisms, including saccharomyces cerevi ...201626990278
dihydroxyacetone production in an engineered escherichia coli through expression of corynebacterium glutamicum dihydroxyacetone phosphate dephosphorylase.dihydroxyacetone (dha) has several industrial applications such as a tanning agent in tanning lotions in the cosmetic industry; its production via microbial fermentation would present a more sustainable option for the future. here we genetically engineered escherichia coli (e. coli) for dha production from glucose. deletion of e. coli triose phosphate isomerase (tpia) gene was carried out to accumulate dihydroxyacetone phosphate (dhap), for use as the main intermediate or precursor for dha produ ...201626992791
detoxification of acidic biorefinery waste liquor for production of high value amino acid.the current study evaluates the detoxification of acid pretreatment liquor (apl) using adsorbent (ads 400 & ads 800) or ion-exchange (a-27mp & a-72mp) resins and its potential for amino acid production. the apl is generated as a by-product from the pretreatment of lignocellulosic biomass and is rich monomeric sugars as well as sugar degradation products (fermentation inhibitors) such as furfural and hydroxymethyl furfural (hmf). of the four resins compared, ads 800 removed approximately 85% and ...201626996259
spie interacts with corynebacterium glutamicum whce and is involved in heat and oxidative stress responses.the gene whce in corynebacterium glutamicum positively responds to oxidative and heat stress. to search for proteins that interact with whce, we employed a two-hybrid system with whce as the bait. sequencing analysis of the isolated clones revealed peptide sequences, one of which showed high sequence identity to a hydrophobe/amphiphile efflux-1 family transporter encoded by ncgl1497. the interaction of the ncgl1497-encoded protein with whce in vivo was verified using reporter gene expression by ...201626996627
transcriptome and gene ontology (go) enrichment analysis reveals genes involved in biotin metabolism that affect l-lysine production in corynebacterium glutamicum.corynebacterium glutamicum is widely used for amino acid production. in the present study, 543 genes showed a significant change in their mrna expression levels in l-lysine-producing c. glutamicum atcc21300 than that in the wild-type c. glutamicum atcc13032. among these 543 differentially expressed genes (degs), 28 genes were up- or downregulated. in addition, 454 degs were functionally enriched and categorized based on blast sequence homologies and gene ontology (go) annotations using the blast ...201627005618
enhancing pentose phosphate pathway in corynebacterium glutamicum to improve l-isoleucine production.three genes, gnd, pgl, and fbp, relevant to the pentose phosphate pathway (ppp) were overexpressed in corynebacterium glutamicum iwj001, leading to increase of l-isoleucine production. the transcriptional levels of gnd, pgl, and fbp significantly increased in iwj001/pdxw-8-gnd-fbp-pgl. compared with the control strain iwj001/pdxw-8, intracellular nadph/nadp(+) ratios in iwj001/pdxw-8-gnd and iwj001/pdxw-8-gnd-fbp cells grown for 36 h increased threefold and fourfold, respectively, indicating tha ...201627010514
co-expression of endoglucanase and β-glucosidase in corynebacterium glutamicum dm1729 towards direct lysine fermentation from cellulose.the aim of the present study is the development of a consolidated bioprocess for the production of lysine with recombinant corynebacterium glutamicum dm1729 strains expressing endoglucanase and β-glucosidase genes. here, the endoglucanase genes from xanthomonas campestris xcc3521 and xcc2387 and betaglucosidase gene from saccharophagus degradans sde1394 were cloned in c. glutamicum dm1729 and expressed either extracellularly or on cell surface. the highest β-glucosidase activity of 9±0.5u/od600 ...201627020126
characterization of aspartate kinase and homoserine dehydrogenase from corynebacterium glutamicum iwj001 and systematic investigation of l-isoleucine biosynthesis.previously we have characterized a threonine dehydratase mutant td(f383v) (encoded by ilva1) and an acetohydroxy acid synthase mutant ahas(p176s, d426e, l575w) (encoded by ilvbn1) in corynebacterium glutamicum iwj001, one of the best l-isoleucine producing strains. here, we further characterized an aspartate kinase mutant ak(a279t) (encoded by lysc1) and a homoserine dehydrogenase mutant hd(g378s) (encoded by hom1) in iwj001, and analyzed the consequences of all these mutant enzymes on amino aci ...201627033538
l-lysine production independent of the oxidative pentose phosphate pathway by corynebacterium glutamicum with the streptococcus mutans gapn gene.we have recently developed a corynebacterium glutamicum strain that generates nadph via the glycolytic pathway by replacing endogenous nad-dependent glyceraldehyde 3-phosphate dehydrogenase (gapa) with a nonphosphorylating nadp-dependent glyceraldehyde 3-phosphate dehydrogenase (gapn) from streptococcus mutans. strain re2, a suppressor mutant spontaneously isolated for its improved growth on glucose from the engineered strain, was proven to be a high-potential host for l-lysine production (taken ...201627044449
mycothiol peroxidase mpx protects corynebacterium glutamicum against acid stress by scavenging ros.to investigate mycothiol peroxidase (mpx) of corynebacterium glutamicum that is a novel cysgpx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress.201627053080
enhanced succinic acid production in corynebacterium glutamicum with increasing the available nadh supply and glucose consumption rate by decreasing h(+)-atpase activity.to enhance succinic acid production in corynebacterium glutamicum by increasing the supply of nadh and the rate of glucose consumption by decreasing h(+)-atpase activity.201627053082
influence of nanomechanical stress induced by zno nanoparticles of different shapes on the viability of cells.there is growing interest in nanostructures interacting with living organisms. however, there are still no general rules for the design of biocompatible nanodevices. here, we present a step towards understanding the interactions between nanostructures and living cells. we study the influence of nanomechanical stress induced by zinc oxide (zno) nanostructures of different shapes on the viability of both prokaryotic (gram-negative bacteria: escherichia coli and enterobacter aerogenes, and gram-pos ...201627074722
the pupylation machinery is involved in iron homeostasis by targeting the iron storage protein ferritin.the balance of sufficient iron supply and avoidance of iron toxicity by iron homeostasis is a prerequisite for cellular metabolism and growth. here we provide evidence that, in actinobacteria, pupylation plays a crucial role in this process. pupylation is a posttranslational modification in which the prokaryotic ubiquitin-like protein pup is covalently attached to a lysine residue in target proteins, thus resembling ubiquitination in eukaryotes. pupylated proteins are recognized and unfolded by ...201627078093
[effect of amn gene deletion on corynebacterium glutamicum s9114 metabolism].to study the effect of adenosine monophosphate nucleosidase gene deletion on corynebacterium glutamicum s9114 metabolism.201527101699
properties of cassava starch modified by amylomaltase from corynebacterium glutamicum.amylomaltase (α-1,4-glucanotransferase, am; ec 2.4.1.25) from corynebacterium glutamicum expressed in escherichia coli was used to prepare the enzyme-modified cassava starch for food application. about 5% to 15% (w/v) of cassava starch slurries were incubated with 1, 3, or 5 units of amylomaltase/g starch. apparent amylose, amylopectin chain length distribution, thermal properties, freeze-thaw stability, thermo-reversibility, and gel strength of the obtained modified starches were measured. the ...201627105125
updates on industrial production of amino acids using corynebacterium glutamicum.l-amino acids find various applications in biotechnology. l-glutamic acid and its salts are used as flavor enhancers. other l-amino acids are used as food or feed additives, in parenteral nutrition or as building blocks for the chemical and pharmaceutical industries. l-amino acids are synthesized from precursors of central carbon metabolism. based on the knowledge of the biochemical pathways microbial fermentation processes of food, feed and pharma amino acids have been developed. production str ...201627116971
transcriptional regulation of the β-type carbonic anhydrase gene bca by rama in corynebacterium glutamicum.carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to bicarbonate and maintains the balance of co2/hco3- in the intracellular environment, specifically for carboxylation/decarboxylation reactions. in corynebacterium glutamicum, two putative genes, namely the bca (cg2954) and gca (cg0155) genes, coding for β-type and γ-type carbonic anhydrase, respectively, have been identified. we here analyze the transcriptional organization of these genes. the transcriptional start site (t ...201627119954
recent advances in amino acid production by microbial cells.amino acids have been utilized for the production of foods, animal feeds and pharmaceuticals. after the discovery of the glutamic acid-producing bacterium corynebacterium glutamicum by japanese researchers, the production of amino acids, which are primary metabolites, has been achieved using various microbial cells as hosts. recently, metabolic engineering studies on the rational design of amino acid-producing microbial cells have been successfully conducted. moreover, the technology of systems ...201627151315
three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (-)-pinoresinol via kinetic resolution.pinoresinol is a high-value plant-derived lignan with multiple health supporting effects. enantiomerically pure pinoresinol can be isolated from natural sources, but with low efficiency. most chemical and biocatalytic approaches that have been described for the synthesis of pinoresinol furnish the racemic mixture. in this study we devised a three-step biocatalytic cascade for the production of enantiomerically pure pinoresinol from the cheap compound eugenol. two consecutive oxidations of eugeno ...201627160378
effect of corynebacterium glutamicum on livestock material burial treatment.in recent years, foot-and-mouth disease has occurred in all parts of the world. the animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans, livestock, and the environment from being affected with the disease. the treatment of corynebacterium glutamicum is a feasible method to reduce the risk of carcass decomposition a ...201627160580
regulation of coenzyme a biosynthesis in the hyperthermophilic bacterium thermotoga maritima.regulation of coenzyme a (coa) biosynthesis in bacteria and eukaryotes occurs through feedback inhibition targeting type i and type ii pantothenate kinase (pank), respectively. in contrast, the activity of type iii pank is not affected by coa. as the hyperthermophilic bacterium thermotoga maritima harbors only a single type iii pank (tm-pank), here we examined the mechanisms that regulate coa biosynthesis in this organism. we first examined the enzyme responsible for the ketopantoate reductase ( ...201627161115
overexpression of ppc and lysc to improve the production of 4-hydroxyisoleucine and its precursor l-isoleucine in recombinant corynebacterium glutamicum ssp. lactofermentum.4-hydroxyisoleucine (4-hil) exhibits unique insulinotropic and insulin-sensitizing activities and is an attractive candidate for the treatment of type ii and type i diabetes. in our previous study, l-isoleucine dioxygenase gene (ido) was cloned and overexpressed in an l-isoleucine-producing strain, corynebacterium glutamicum ssp. lactofermentum sn01, and 4-hil was produced from the endogenous l-isoleucine (ile). in this study, ppc and lysc were co-expressed with ido to increase the supply of ile ...201627178798
engineering of corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars.xylitol is a non-fermentable sugar alcohol used as sweetener. corynebacterium glutamicum atcc13032 was metabolically engineered for xylitol production from the lignocellulosic pentose sugars xylose and arabinose. direct conversion of xylose to xylitol was achieved through the heterologous expression of nad(p)h-dependent xylose reductase (xr) gene from rhodotorula mucilaginosa. xylitol synthesis from arabinose was attained through polycistronic expression of l-arabinose isomerase (araa), d-psicos ...201627184428
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