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[toxic effect of bombyx mori of b. thuringiensis parasporal bodies and culture filtrates]. 19704999947
autolysis of bacillus cereus cell walls and isolation of structural components.autolysis of bacillus cereus n.r.r.l. 569 cell walls was accompanied by hydrolysis of the majority of the 4-o-beta-n-acetylglucosaminyl-n-acetylmuramic acid linkages in mucopeptide, presumably by an endo-beta-n-acetylglucosaminidase. hydrolysis of the n-acetylmuramyl-l-alanine linkages by an amidase also occurred. free d-alanine residues were detected in isolated cell walls and the proportion of these residues increased during autolysis, presumably due to d-alanine carboxypeptidase action. fract ...19715000275
genetic evidence for heterozygotes in mixed culture of bacillus cereus. 19705000288
uranyl salts as fixatives for electron microscopy. study of the membrane ultrastructure and phospholipid loss in bacilli. 19715000342
comparison of bacillus cereus bacteriophages cp-51 and cp-53.transducing bacteriophages cp-51 and cp-53 were compared. unlike cp-51, cp-53 appeared to be a lysogenizing phage. cp-51 gave greater frequencies of co-transduction for linked markers than did cp-53. cp-51 was found to be a larger phage which carried more deoxyribonucleic acid (dna) than cp-53. cp-51 dna contained about 43% guanine plus cytosine and in addition contained 5-hydroxymethyluracil in place of thymine. cp-53 dna contained no unusual bases; its guanine plus cytosine content was 37%.19715000543
the interconvertibility of various bacterial transfer ribonucleic acids between an active and an inactive stable configuration. 19715000606
the role of the histidine side chains in the catalytic activity of b. cereus expopenicillinase. 19715000681
aerobic microbes isolated from slaughtered pigs and their public health importance. 19705001153
in vivo and in vitro action of new antibiotics interfering with the utilization of n-acetyl-glucosamine-n-acetyl-muramyl-pentapeptide.recent literature on the antibiotics enduracidin, moenomycin, prasinomycin, and 11.837 rp suggested an interaction with murein synthesis. incubation of sensitive strains from bacillus cereus and staphylococcus aureus in a "wall medium" containing labeled l-alanine showed that all four antibiotics inhibited the incorporation of alanine into murein and gave rise to accumulation of radioactive uridine diphosphate-n-acetyl-muramyl (udp-murnac)-pentapeptide. peptidoglycan was synthesized when the par ...19715001198
microstructure of colonies of rod-shaped bacteria.whole colonies of bacillus cereus, b. megaterium, b. mycoides cn2495, corynebacterium hofmanni nctc1938, escherichia coli, lactobacillus acidophilus ncib1899, nocardia graminis nctc4728, pseudomonas viscosa, and serratia marcescens were prepared for scanning electron microscopic examination by freeze-drying and metal-coating. the arrangement of individual cells within colonies could be seen. cells of bacillus colonies tended to be longer than in liquid culture and irregular in shape and to give ...19715001203
reconstitution of bacterial spore coat layers in vitro.the spore coat layers of bacillus cereus t can be at least partially restored by mixing spores stripped of their coat with solubilized coat protein.19715001206
biochemical aspects of the visual process. 13. the role of phospholipids in cattle rhodopsin studied with phospholipase c. 19715001225
macromolecular sieving by the dormant spore of bacillus cereus.the threshold surface porosity in the dormant spore of bacillus cereus strain t was assessed by measuring passive permeabilities to a series of polydisperse polyethylene glycol samples which increased in average molecular size. the apparent exclusion threshold at diffusional equilibrium corresponded to a polymer of number-average molecular weight ( m(n)) = 150,000 and equivalent hydrodynamic radius ( r(es)) = 16 nm, which confirmed a previous report. however, analytical gel chromatography before ...19715001873
effect of oxidation on the transition of penicillinase conformation. 19715001985
[action of phospholipase c on diol phospholipids]. 19715001986
occurrence of lysophosphatides in bacteriophage t4rii-infected escherichia coli s-6.hydrolysis of phospholipids was observed to start about 15 min after escherichia coli s/6 cells were infected with t4rii bacteriophage mutants. hydrolysis continued through the latent period and well past the time when cell lysis occurs. the hydrolytic products that accumulated were free fatty acids, 2-acyl lysophosphatidylethanolamine, and various lysocardiolipins. these products indicated the action of phospholipase a(1). from 15 to 22 min after infection, there were equivalent amounts of fatt ...19715002011
phospholipase c from pseudomonas fluorescens. 19715002152
[determination of the degree of adhesion of microbial cells to solid surfaces]. 19715002169
the outgrowing bacterial endospore as a system for the study of cellular differentiation. 19695002258
the antimicrobial activity of citral. 19715002370
bacillus cereus neutral protease. 19715002444
the interrelation of polar lipids in bacterial membranes. 19715002559
lung tissue and serum levels of methacycline. 19715002640
inhibition of growth and rna biosynthesis of bacillus cereus by quinacrine. 19715002781
selective effect of salicylate on microbial rna biosynthesis. 19705002789
the "sensitivity" of resting and dividing cells. 19715002845
[mechanism of regulation of alpha-acetohydroxyacid-synthetase in bacillus cereus t]. 19715002914
effects of fluorouracil on rna synthesis in bacillus cereus. 19715002954
changes induced in the ultrastructure of the cytoplasmic and intracytoplasmic membranes of several gram-positive bacteria by variations in oso 4 fixation. 19715003136
[experimental data on the study of the stability of streptotricin antibiotics phytobacteriomycin and polymycin]. 19715003153
the interrelation of phosphatidylethanolamine and glycosyl diglycerides in bacterial membranes. 19715003473
circular-dichroism studies on two -lactamases from bacillus cereus.the circular-dichroism (cd) spectra of beta-lactamases i and ii from bacillus cereus 569/h are reported, along with that of the beta-lactamase ii free from carbohydrate. the results show that carbohydrate makes an appreciable contribution to the optical activity of beta-lactamase ii in the far-ultraviolet, and that removal of carbohydrate greatly affects the optical activity of several aromatic side chains of the protein moiety. both tyrosyl and tryptophanyl residues are affected, showing that s ...19715003529
bacteria and actinomycetes antagonistic to fungal root pathogens in australian soils. 19715003563
the "sensitivity" of resting and dividing cells. 19705003582
incorporation of penicillinase into reaggregated mycoplasma membranes. 19715003616
action of pure phospholipase a 2 and phospholipase c on human erythrocytes and ghosts. 19715003696
the effect of substrate analogues on the iodine sensitivity of penicillinase from b. cereus. 19715003710
effect of electrolytes on the activity and iodine sensitivity of penicillinase from b. cereus. 19715003711
[the detection, by biological means of low levels of glucane in gelose]. 19715003775
[montreal episode of food poisoning due to bacillus cereus]. 19715003957
effect of urea on the two iodine inactivation reactions of bacillus cereus penicillinase. 19715004078
effect of pyrazocillin on the induced penicillinase synthesis of bacillus cereus. 19715004081
water limited reaction of bacillus cereus penicillinase in nonaqueous solvent. 19715004082
some properties of the membrane-bound penicillinase of bacillus cereus 569. 19715004083
genetic relatedness within the bacillus cereus group of bacilli. 19715004162
the effect of polyethyleneglycol on the iodine-sensitivity of penicillinase from b. cereus. 19715004272
the factors affecting the stability of the penicillinase messenger rna of b. cereus 569. 19715004273
the effect of induced withdrawal on the penicillinase synthesis induced aspecifically with phosphate in b. cereus. 19715004274
[survival of bacillus cereus in food products subjected to various types of cooking processes]. 19715004282
comparative dna-rna hybridization in differentiated cells. 19715004356
inhibition of l-alanine-induced germination of bacillus cereus spores by theophylline. 19715004488
the outgrowing bacterial endospore as a system for the study of cellular differentiation. 19695004614
[microbiological titration of a number of tetracycline derivatives in newly-formed osseous tissues in the young of albino mice]. 19695004703
[formation and determination of nitric oxide in sterile, canned spinach]. 19715004938
irreversible adsorption of exopenicillinase on bacillus cereus 569 cells. 19715005043
[research on the dissociating activity of some constituents of vitreous humor]. 19715005105
[comparative study of bacillus cereus sporogenesis in stable, running, and stable-running microcultures]. 19715005136
purification and specificity of a lytic enzyme isolated from b. cereus. 19715005194
[effect of entobacterine-3 and biotrol-183 on laspeyresia pomonella l. catterpillars]. 19715005233
[adsorption of bacteria on glass, modified glass surfaces and polymer films]. 19715005572
preparation and purification of microbial proteases with special reference to the bacilli. 19715005723
drug dependence and phenotypic masking in gram positive and gram negative bacteria. 19715005839
observations on biochemical changes in irradiated spores of bacillus cereus. 19655006489
the purification and properties of a penicillinase whose synthesis is mediated by an r-factor in escherichia coli.1. the penicillinase (beta-lactamase) from escherichia coli strain tem has been purified and its activity against a range of penicillin and cephalosporin derivatives measured. 2. the enzyme shows little resemblance to penicillinases from bacillus cereus, bacillus licheniformis and staphylococcus aureus. 3. the molecular weight of the enzyme is 16700+/-5%, which is about half the value obtained for other penicillinases. 4. the enzyme is most similar in properties to a crude preparation of a penic ...19665328167
effect of gas hydrate formers on microorganisms.various bacteria, yeasts, and molds important to the food industry were incubated in aerosol cans containing a c broth and one of the following three gas hydrate formers: propane, dichlorodifluoromethane (f-12), and 1,1-difluoro-1-chloroethane (f-142b). most hydrate formers were tested at three concentrations: low (vapor state), intermediate (liquid state, low level), and high (liquid state, high level). samples were continuously agitated for 48 hr at 21 +/- 3 c. changes in numbers of microorgan ...19705456934
occurrence of crithidia factors and folic acid in various bacteria.crithidia factors and folic acid were found to be widely distributed in culture fluids and in cells of 27 species of bacteria, when cultured under aerobic conditions into the stationary phase. most bacteria excreted more crithidia factors and folic acid than they retained in their cells. one crithidia factor produced by serratia indica and one produced by bacillus cereus differed from biopterin in their chromatographic behavior. the factor excreted by s. indica appeared to be a 2-amino-4-hydroxy ...19705473887
use of cryptograms for characterization of strains of the bacillus thuringiensis-bacillus cereus group. 19715575752
food-borne bacterial toxins.food poisoning caused by the ingestion of preformed bacterial toxins is considered in relation to comparative symptoms, procedures for extraction and purification of the causal toxins, their chemistry, serology, assay procedures and pharmacology, in so far as these are known.the bacteria discussed in this context are clostridium botulinum, c. perfringens, staphylococcus aureus, bacillus cereus, and vibrio parahemolyticus. the possible roles of the enterococci, proteus, e. coli and of unknown spe ...19665905949
influence of food microorganisms on staphylococcal growth and enterotoxin production in meat.forty-four microorganisms were studied for their influence on staphylococcal growth and enterotoxin production. inhibition was found to be more common than stimulation. two types of inhibition were observed: inhibition of staphylococcal growth, and inhibition of enterotoxin formation with no apparent effect on growth. by use of a plate test, 12 of the 44 food microorganisms were found to inhibit staphylococcal growth at 35 c. of the 12, 3 also inhibited growth at 25 c. no significant differences ...19665970822
regulation of glutamine synthetase. ii. patterns of feedback inhibition in microorganisms.the feedback inhibition of glutamine synthetase was investigated by use of partially purified enzyme preparations from salmonella typhimurium, micrococcus sodonensis, pseudomonas fluorescens, bacillus cereus, bacillus licheniformis, clostridium pasteurianum, rhodospirillum rubrum, neurospora crassa, candida utilis, and chlorella pyrenoidosa. inhibition analyses indicated that the enzyme of each organism can be effectively regulated with mixtures of end products from the diverse pathways of gluta ...19676025412
selection of a beta-amylase-producing strain and its fermentation conditions. 19846084437
an sau3 ai restriction endonuclease isoschizomer from bacillus cereus.the isolation and characterization of a restriction endonuclease from bacillus cereus ioc 243 are described. the enzyme recognizes the palindromic sequence 5'-g(met-a,a)tc-3' as determined by pei chromatography of pancreatic dnase, snake venom phosphodiesterase digestion products of labelled fragments, analysis of restriction digests from normal and n6-methyladenine-free dna and direct sequence analysis of cloned fragments. the staggered cleavage products with 5' -terminal pgatc extensions are e ...19846086403
structural studies of fc receptors. v. effect of phospholipase c treatment on the binding activities of the fc receptor of macrophage or its isolated plasma membrane.the effect of phospholipase c treatment on the binding activity of the fc receptor of guinea pig macrophage was studied to analyze the interaction of the fc receptor with membrane phospholipids necessary for the activity. it was confirmed by subcellular fractionation that the receptor is localized on the plasma membrane. treatment of the whole cell or isolated plasma membrane with phospholipase c of clostridium perfringens diminished the binding of soluble igg2-immune complex to fc receptors on ...19846088472
steroidogenic effect of exogenous phospholipase c on bovine adrenal fasciculata cells.phospholipase c (bacillus cereus) added to the incubation medium stimulated the steroidogenic activity of bovine adrenal zona fasciculata cell suspensions to a level similar to that induced by optimal concentration of acth. this effect was not related to an increase of cyclic amp; it was calcium-dependent and was also induced by an other bacterial phospholipase c (from clostridium perfringens) whereas phospholipases a2 and d were ineffective. phospholipid metabolism was examined in these cells a ...19846089796
evidence for the role of calcium and diacylglycerol as dual second messengers in thyrotropin-releasing hormone action: involvement of diacylglycerol.trh stimulates the secretion of prl by clonal gh3 pituitary cells. the studies of the accompanying paper have shown that the secretory response during the first 30-60 min is biphasic (phase i, 0-3 min; phase ii, 5-60 min) and that the phase i response may be mediated through mechanisms involving ca+2 translocation. in previous studies, it has been shown that trh treatment rapidly induces the breakdown of inositol phospholipids with accompanying diacylglycerol accumulation. in this paper, we pres ...19846090104
[enterotoxins of bacillus cereus and clostridium perfringens]. 19846097703
dna-dependent atpase ii from bacillus cereus.a new dna-dependent atpase been purified close to homogeneity from soluble extracts of bacillus cereus. this enzyme, called atpase ii catalyses the hydrolysis of atp in the presence of mg2+ or ca2+ and dna. single-stranded linear dna is a cofactor about 3-fold more effective than double-stranded dna. the enzyme catalyses the strand separation of duplex dna in the presence of atp. however, at concentrations higher than 0.5 mm, phosphohydrolysis can occur without concomitant dna unwinding. the enz ...19806107127
the structure of beta-lactamases.the beta-lactamases are widely distributed in both gram-positive and gram-negative bacteria. they all inactivate penicillins and cephalosporins by opening the beta-lactam ring. many varieties of the enzyme can be distinguished on the basis of their catalytic and molecular properties, but only amino acid sequence determination gives information upon which a molecular phylogeny can be based. the present evidence suggests that the beta-lactamases have a polyphyletic origin. all the beta-lactamases ...19806109327
active sites of beta-lactamases from bacillus cereus.there are two extracellular beta-lactamases produced by bacillus cereus 569. one of these enzymes, beta-lactamase i, is inactivated by 6-beta-bromopenicillanic acid: the site of reaction is serine-44. this is a conserved amino acid residue in the other beta-lactamases whose structures have been determined, and it becomes a good candidate for an active-site group in these enzymes. the inactivation may involve a rearrangement leading to a dihydrothiazine. the other extracellular enzyme produced by ...19806109328
isolation and properties of pili from spores of bacillus cereus.structures whose morphology is identical to that of bacterial pili have been isolated from spores of bacillus cereus. the structures are absent from log-phase and sporulating cells. the pili are 6.8 nm in diameter, are of variable length, and appear to emanate randomly from the exosporium. examination of spores from 12 bacillus species showed that only those from b. cereus and b. thuringiensis have pili. although isolated spore pili were shown to be composed of protein, their subunit nature was ...19816109707
bacillus cereus meningitis in a patient under gnotobiotic care. 19816116070
a third dna-dependent atpase from bacillus cereus free of atp-dependent dnase activity.the purification of atp-dependent dnase from bacillus cereus led to the isolation and characterization of a third dna-dependent atpase. the enzyme called atpase iii has been purified free of nuclease activity. none of the expected atpases proved to be identical with atp-dependent dnase-dna-dependent atpase. separation of atpase i, ii and iii and a dnase specific for single-stranded dna from the same source excludes the possibility of atp-dependent dnase being the action of a single enzyme molecu ...19836134644
bacillus cereus as a systemic pathogen. 19836140557
preferential suppression of normal exoenzyme formation by membrane-modifying agents.cerulenin, phenethyl alcohol, benzyl alcohol, procaine, and a series of aliphatic alcohols selectively suppressed production of active exoenzymes by various bacterial strains.19806154046
the mode of action of nanaomycin a in gram-positive bacteria.the mode of action of nanaomycin a on gram-positive such as staphylococcus aureaus, bacillus cereus and streptococcus faecalis was investigated. nanaomycin a inhibited the biosyntheses of protein, dna, rna and cell-well peptidoglycan to a similar extent. it increased the oxygenous respiration of s. aureus cells at the minimal inhibitory concentration. the cells preincubated with nanaomycin a showed stimulation of proton influx after addition of n,n'-dicyclo-hexylcazrbodiimide, an inhibitor of ca ...19806159343
phospholipase c (bacillus cereus) digestion of phospholipids in myelin isolated from central nervous tissue: removal of myelin basic protein increases exposure of lipids to the enzyme. 19806161042
specificity of translation of spore polypeptides in bacillus in vitro systems.cell free extracts prepared from exponentially growing escherichia coli and bacillus cereus as well as from bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with bacillus ribonucleic acid. all three preparations synthesized glutamine synthetase antigen when ribonucleic acid from a bacillus subtilis strain that overproduces glutamine synthetase was added. the post exponential bacillus cereus extract, however, ...19816165330
evidence for the presence of subpopulations of arenicola marina coelomocytes identified by their selective response towards gram+ve and gram-ve bacteria.in arenicola marina (l.), in vitro attachment and phagocytosis of bacteria appears to be serum-independent and thus, non-specific. mixtures of gram+ve and gram-ve bacterial species (gram+ve/-ve, of two gram+ve (gram+ve/+ve) and of two gram-ve bacterial species (gram-ve/-ve)were overlaid on coelomocyte monolayers, in order to introduce competition for cell surface 'receptors'. an enhanced total phagocytosis was only recorded in the gram+ve/-ve mixtures in comparison with their controls. the resul ...19826175542
bacillus cereus cross-infection in a maternity-unit. 19816175691
variations on the staining method in quantitative indirect immunofluorescence assays for bacillus spores, and the use of fluorescein--protein a.quantitative indirect immunofluorescence assays for b. anthracis and b. cereus spores fixed on multispot microscope slides have been performed using a microfluorometer to measure the fluorescence of individual bacteria. a study has been made of variations of the indirect assay sequence, in which the washing operation between application of 1st and 2nd antibody types was omitted. in one modification the addition of the indirect antibody was deferred, and in the second the direct and indirect reag ...19826184416
isolation and preliminary characterization of streptococcus cremoris (strain 202) bacteriocin.ability to produce bacteriocin (lactostrepcin) is demonstrated in streptococcus cremoris. however, lactostrepcin was found to be produced by only one strain (strain 202) out of the 17 s. cremoris strains tested. this bacteriocin is preliminarily characterized, it differs from other lactic streptococci bacteriocins. the strain 202 bacteriocin acts on many strains of lactic streptococci, strains of bacillus cereus, and some strains of leuconostoc and lactobacillus. it was shown that strain 202 con ...19836196942
a method for estimation of the antibiotic activity of streptomyces griseus and streptomyces lactamdurans strains producing cephamycins. 19836202109
metabolic change in cultured gingival fibroblasts exposed to bacterial extracts. stimulation of hyaluronic acid synthesis. 19846235339
commercial milk products and indigenous weaning foods in a rural west african environment: a bacteriological perspective.two commercially available baby milks, one 'biologically acidified', the other 'non-acidified', and a traditional weaning food, millet gruel, were prepared and stored under village conditions in west africa. increases in total colony count and in number of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in these products when stored as commonly practised at ambient temperatures over a period of 8 h. poor hygiene during preparation was indicated by ...19806244343
studies on sphingomyelinase of bacillus cereus: hydrolytic and hemolytic actions on erythrocyte membranes. 19806244783
rapid spectrophotometric method for the determination of beta-lactamase activity. 19806247317
cross-linking preserves conformational changes induced in penicillinase by its substrates.exopenicillinase of bacillus cereus 569/h was cross-linked with toluene 2,4-diisocyanate in the presence of cephalothin, cloxacillin or no substrate. the derivatives show significant differences in susceptibility to inactivation by heat, urea, iodination or proteolysis. such differences can be predicted from the contrasting effects of these substrates on the conformation of the enzyme.19806249271
spectrophotometric determination of inhibitory effects of cp-45899 on beta-lactamase with benzylpenicillin and nitrocefin. 19806249784
inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid: kinetics.the kinetics of the inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid are described. loss of beta-lactamase activity is accompanied by a decrease in protein fluorescence, by the appearance of a protein-bound chromophore at 326 nm, and by loss of tritium from 6 alpha-[3h]-6 beta-bromopenicillanic acid. it is shown that all of the above changes probably have the same rate-determining step. the inactivation reaction is competitively inhibited by cephalosporin c, a co ...19806250581
induction of accelerated sporangial lysis by basic peptide antibiotics. a novel method of preparation of free spores of bacilli. 19806253430
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