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the midcycle cervical microbial flora as studied by the weighed-swab method, and its possible correlation with results of sperm cervical mucus penetration tests.infertile couples undergoing routine investigation for infertility were randomly selected for the study. a quantitative method using weighed swabs was found satisfactory for the study of microbial flora in the midcycle cervical mucus. in 6 of the 20 women, the midcycle cervical mucus was hostile in the sperm cervical mucus penetration tests. the total bacterial counts and the number of species (average, 2.5) isolated from the hostile mucus were significantly greater than those from the receptive ...19873109958
[an intravenous penicillin-erythromycin lactobionate combination in the treatment of bacterial endocarditis caused by enterococcus]. 19873110650
penetration of bacteria and spermatozoa into bovine cervical mucus.it has been reported that bacteria may attach to motile spermatozoa, be carried through the cervix and uterus to the fallopian tubes, and cause acute salpingitis. in an attempt to mimic these conditions in vitro, we incubated escherichia coli, streptococcus faecalis, pseudomonas aeruginosa, and staphylococcus aureus with motile spermatozoa which then were allowed to migrate through a capillary filled with bovine cervical mucus. after satisfactory sperm migration through the mucus, the capillarie ...19873110711
therapeutic effects of imipenem-cilastatin on experimental intrauterine infections in rats.the therapeutic effects of imipenem-cilastatin (mk-0787-mk-791) on experimental intrauterine infections in progesterone-treated virgin rats and postpartum rats were studied. the relative efficacy of imipenem-cilastatin for the treatment of such intrauterine infections was compared with that of cefazolin and ampicillin for the treatment of infections caused by escherichia coli and streptococcus faecalis, respectively. treatment with imipenem-cilastatin significantly inhibited the proliferation of ...19873111355
failure to demonstrate a consistent in vitro bactericidal effect of trimethoprim-sulfamethoxazole against enterococci.controversy exists as to the in vitro and in vivo activities of trimethoprim-sulfamethoxazole (tmp-smx) against enterococci. in this study, we investigated the in vitro activity of tmp-smx in the type of mueller-hinton broth previously reported to give the lowest mics and mbcs with enterococci. in all instances, mics were less than or equal to 0.5 microgram/ml. the majority of tests showed mbcs of greater than 32 micrograms/ml, although there was some effect from varying the inoculum and the len ...19873111362
comparative survival of antibiotic-resistant and -sensitive fecal indicator bacteria in estuarine water.the survival of antibiotic-resistant and -sensitive strains of escherichia coli, enterococcus faecalis, enterococcus faecium, streptococcus equinus, and two environmental isolates, ap17 and aq62, was examined in estuarine water. each strain was rendered resistant to a combination of two antibiotics by serial passage in increasing concentrations of antibiotics. cultures were incubated in filter-sterilized estuarine water for up to 7 days. recovery was assessed by examining colony-forming ability ...19873111372
comparison of single and multiple doses of prophylactic antibiotics in experimental streptococcal endocarditis.single-doses or short-term administration of beta-lactam antibiotics alone or combined with aminoglucoside antibiotics have failed to consistently prevent experimental streptococcal endocarditis induced by high inocula of bacteria poorly susceptible to killing by these antibiotics. the optimal duration of administration of antibiotics for successful prophylaxis under these circumstances has not been established. we therefore tested, in rats with catheter-induced sterile aortic vegetations, the d ...19873111743
comparison of most probable number and pour plate procedures for isolation and enumeration of sulphite-reducing clostridium spores and group d faecal streptococci from oysters.a comparative study of methods to enumerate sulphite-reducing clostridium spores and group d faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. reinforced clostridial broth with supplements to detect sulphite reduction was compared with pour plates of egg yolk-free tryptose sulphite cycloserine agar incubated at 37 degrees c for 24 h. azide dextrose broth was compared with pour plates using slane ...19873112087
the effect of ampicillin and tylosin on the faecal enterococci of healthy young chickens.enterococcal isolates from young chickens were differentiated into one of three species, namely enterococcus faecalis, ent. faecium and ent. gallinarum. the proportion of each species among the enterococcal population changed with time in birds not dosed with antibiotics. this pattern of change was modified in birds dosed with either tylosin or ampicillin even though ampicillin did not select for ampicillin resistance among the enterococcal population. a gradual increase in tylosin resistance wa ...19873112089
in vitro activity of ro 19-5247 (t-2525) and interpretive criteria for disk diffusion susceptibility testing.the activity of ro 19-5247 (the active metabolite of the oral cephalosporin ro 19-5248 [t-2588]) was compared with that of five orally active agents against a total of 331 bacterial strains. ro 19-5247 was more active in vitro than amoxicillin, amoxicillin-clavulanate, cefaclor, cefuroxime, and cephalexin against members of the family enterobacteriaceae. amoxicillin-clavulanate and amoxicillin overall were more active than the other four agents against staphylococci. ro 19-5247, amoxicillin-clav ...19873112176
strain-dependent difference in susceptibility of mice to experimental ascending pyelonephritis. 19873112242
[study of the pathogenicity of enterococcus faecalis--relationship between infectibility of e. faecalis in mice and its biological activity]. 19873112286
[clinical and experimental studies of the pathogenicity of enterococcus faecalis in the urinary tract]. 19873112456
experimentally induced bacteroides infections in the rabbit.rabbits were infected with bacteroides fragilis, b. macacae and b. gingivalis in monoinfection, mixed bacteroides infection, and mixed infection of b. gingivalis with streptococcus faecalis, escherichia coli and clostridium sporogenes. monoinfection gave rise to localised, nodular abscesses at cell levels greater than 0.5 x 10(5) cfu ml-1, the severity of which was dose related. mixed infections including b. gingivalis caused severe spreading lesions and affected organs distant from the injectio ...19873112527
proteolytic activity of lactic acid bacteria in skim milk with special reference to the biodegradation of casein fractions.streptococci and lactobacilli were assayed for their proteolytic activity in pasteurised (95 degrees c for 30 min) fresh friesian cows' skim milk incubated at 30 degrees c for 48 h. lactobacilli were more proteolytic than the streptococci except s. faecalis subsp. liquefaciens. s. faecalis and s. thermophilus followed s. lactis subsp. diacetylactis in the proteolytic activity. electrophoretic analysis of the precipitated casein revealed k-, pre-beta- and the small slow band of alpha s1-casein to ...19873112574
[in vitro study of the combination of pefloxacin with other antibiotics against pseudomonas aeruginosa and streptococcus group d].the comparative susceptibility of 50 isolates of enterococci and 50 isolates of pseudomonas aeruginosa to pefloxacin and different antibiotics was tested using mic determination. the results showed only 6% sensitive strains of enterococci and 36% of p. aeruginosa. the high level resistant streptococci against aminoglycosides and resistant pseudomonas strains have weakly higher mic than sensitive strains. the activity of the combination of pefloxacin and other antibiotics was determined by a chec ...19873112710
[effects of loperamide on the fecal flora in children in severe diarrheas].loperamide has been recently indicated in the management of infants with severe protracted diarrhea. the purpose of this study was to determine the effect of loperamide on fecal flora in severe protracted diarrheas. 19 children aged 1 to 36 months, with severe protracted diarrhea were studied: 14 received loperamide (0.5 mg/kg/d) and 5 were without loperamide treatment. criteria analysed were: clinical tolerance (vomiting and abdominal distention); efficacy (number of stools, transit time and na ...19873112712
[effect of aztreonam on the fecal aerobic flora in children].aztreonam, a new monobactam, has a spectrum limited to gram negative aerobic bacilli. to evaluate its effect after parenteral administration on aerobic stool flora (gram negative bacilli, streptococci d, staphylococci, candida), quantitative cultures used serial dilution of stools twice a week on selective media. for gram negative bacilli, agar dishes containing aztreonam or not were used. colonies of different morphologies were counted. representatives of each morphological type were then picke ...19873112714
bacterial interference by anaerobic species isolated from human feces.eighty-four anaerobic fecal isolates obtained from five healthy volunteers were tested for their ability to inhibit in vitro growth of eight species of enterobacteriaceae, four species of faculative gram-positive cocci, and pseudomonas aeruginosa. forty-nine of the 84 anaerobic isolates (58%) inhibited the growth of at least one indicator bacterium. isolates of bacteroides and bifidobacterium spp. were most consistently inhibitory. anaerobic cocci and clostridia were infrequently inhibitory; eub ...19873113937
lowering of cytoplasmic ph is essential for growth of streptococcus faecalis at high ph.the growth of streptococcus faecalis at high ph was significantly stimulated by carbonate. in the absence of added carbonate the cells were unable to grow at a ph above 9.5, but in media containing 50 mm hco3- they grew even at ph 10.5. both rate and yield of growth at ph 9.5 were significantly stimulated by as little as 5 mm carbonate. the cytoplasmic ph in growing cells was maintained at about 7.8 to 8.2, whereas the medium ph ranged from 8.4 to 9.5. nigericin and gramicidin d, ionophores whic ...19873114241
high incidence of hemolysin production by enterococcus (streptococcus) faecalis strains associated with human parenteral infections.hemolysin production, clumping (pheromone) response, transferability of the hemolytic trait, and drug resistance were examined in 97 clinical isolates of enterococcus (streptococcus) faecalis. the isolates were derived from various sources (i.e., urine, pus, vagina, sputum, bile, and blood), and approximately 60% were found to be hemolytic. about 85% of the hemolytic strains exhibited a clumping response, compared with about 49% of the nonhemolytic strains. over 50% of the hemolytic strains carr ...19873114322
in vitro infection and disinfection of dentinal tubules.an in vitro model for dentinal tubule infection of root canals was developed. cylindrical dentin specimens, 4 mm high with a diameter of 6 mm and a canal 2.3 mm wide, were prepared from freshly extracted bovine incisors. the cementum was removed from all dentin blocks. the tubules were opened by four-minute treatments with 17% edta and 5.25% naocl before being infected with enterococcus faecalis atcc 29212 in yeast extract-glucose broth. bacteria rapidly invaded the tubules. after three weeks of ...19873114347
in vitro antibacterial activity of commonly used antibiotics against urinary tract isolates. 19873114512
[cervical spondylitis as a sequela of rubber band ligation treatment of hemorrhoids]. 19873114993
in vivo and in vitro activity of ciprofloxacin plus azlocillin against 12 streptococcal isolates in a neutropenic site model.closed-space neutropenic infection sites were simulated in rabbits by subcutaneous semipermeable chambers that were inoculated with 5 x 10(4) cfu/ml of various strains of streptococcus pneumoniae, streptococcus faecalis, and streptococcus avium. four hours after inoculation, treatment was begun with ciprofloxacin, 10 or 30 mg/kg, azlocillin, 100 mg/kg, amikacin, 15 mg/kg, procaine penicillin g, 300 u/dose, or gentamicin, 2 mg/kg, alone and in two-drug combinations. antimicrobials were given intr ...19873115671
human choriogonadotropin-like material in bacteria of different species: electron microscopy and immunocytochemical studies with monoclonal and polyclonal antibodies.immunocytochemical studies using antisera to whole human choriogonadotropin (hcg), to its alpha- and beta-subunits and to the cooh-terminal peptide of hcg beta, and two monoclonal antibodies to hcg beta, demonstrated expression of hcg-like material, its individual subunits and/or fragments in nine bacterial strains. seven of these were isolated from patients with cancer and were definitely identified as streptococcus faecalis (three strains), staphylococcus haemolyticus (two strains) and staphyl ...19873116165
effect of chlorhexidine/edta/tris against bacterial isolates from clinical specimens.chlorhexidine/edta/tris was more active compared with chlorhexidine against the following species of organisms: acinetobacter species, citrobacter species, enterobacter species, escherichia coli, proteus mirabilis, providence species, pseudomonas species, staphylococcus aureus, staphylococcus epidermidis and streptococcus faecalis, when tested in isosensitest agar. the addition of 20% serum to the medium reduced its effectiveness mainly against providence species, proteus species, and streptococ ...19873116368
[in vitro studies on microbial incorporation of nitrogen from [15n2] urea and [15n]ammonium chloride by human intestinal flora].6 typical bacteria species of the human intestinal flora (e. coli, klebsiella pneumoniae, proteus vulgaris, streptococcus faecalis, bacteroides fragilis, bifidobacterium sp.) were incubated in a liquid medium for 48 h with [15n2]-urea and [15n]-ammonium chloride. the rates of [15n]-incorporation were calculated. they depend reproducible on the species examined, on the kind of the offered npn-substance and on the amount of npn-substance in the medium. with [15n2]-urea the minimal rate of incorpor ...19873116431
[acinetobacter and streptococcus faecalis septicemia with recurring coma, treated with ceftriaxone]. 19873116632
serum bactericidal activity and postantibiotic effect in serum of patients with urinary tract infection receiving high-dose amikacin.ten patients received a 30-min infusion of amikacin (30 mg/kg) on day 1 and 15 mg/kg on day 2. mean serum creatinine was 1.1 +/- 0.3 (standard deviation) mg/dl before and 1.0 +/- 0.3 mg/dl 3 days after the second infusion. mean serum amikacin concentrations before, at the end of infusion, and 1, 6, 12, and 24 h after 30 and 15 mg/kg were 0, 157, 79, 31, 16, 5, 5, 85, 51, 19, 12, and 5 mg/liter, respectively. five strains each of staphylococcus aureus, staphylococcus epidermidis susceptible and r ...19873116918
distribution of indicator bacteria and vibrio parahaemolyticus in sewage-polluted intertidal sediments.the impact of a sewage point source on the bacterial densities in an intertidal mud flat in boston harbor, mass., was investigated. the area, savin hill cove, acts as a receiving basin for a combined storm and sewage outlet (cso). preliminary examination of sediments and overlying water at high tide demonstrated that fecal coliforms were present in sediments at abundances 2 to 4 orders of magnitude higher than in the overlying water column. the following bacterial counts were determined from sed ...19873116932
antibody response and dental caries in the bacterium streptococcus-mono-associated, caries-resistant and caries-susceptible rats.correlations between rat strain, agglutinating antibody responses in serum or saliva and caries incidence, previously observed in harvard rats mono-associated with streptococcus faecalis and given diet l128 and 5 per cent sucrose added to drinking water, were not found when experiments were repeated with the low-sucrose diet 305. mono-association with either streptococcus mutans 6715 or strep. faecalis nd547 produced extensive caries in caries-susceptible (hcs) rats and little or no caries in re ...19873117024
comparative in vitro activity of ro 23-6240 (fleroxacin), a new 4-quinolone derivative.the in vitro activity of ro 23-6240 was compared with that of norfloxacin, ofloxacin and ciprofloxacin as well as four other antimicrobial agents against 345 recent clinical isolates. the mics of ro 23-6240 against enterobacteriaceae and acinetobacter anitratum was less than or equal to 0.5 mg/l. at the same concentration of the compound 90% of staphylococci were inhibited. against enterococcus faecalis and pseudomonas aeruginosa ro 23-6240 proved less active, having mic90 values of 4.0 mg/l and ...19873117538
comparison of physiologic tests used to identify non-beta-hemolytic aerococci, enterococci, and streptococci.twenty-one reference strains and 88 clinical isolates of aerococcus, enterococcus, and streptococcus species were tested for reactions in the rapid strep (rs) and modified conventional tests. we conclude that some but not all of the tests in the rs system could be used to substitute for conventional tests. rs tests for hydrolysis of arginine, esculin, l-pyrrolidonyl-naphthylamide, production of acetyl methyl carbinol (voges-proskauer), and fermentation of arabinose, lactose, mannitol, raffinose, ...19873117840
patterns of wound colonisation in patients with peripheral vascular disease.the bacterial colonisation of ulcers related to vascular disease, amputation sites and sites of surgical vascular repair was studied in 122 patients with vascular disorders. wounds were graded clinically on the basis of inflammation, oedema and exudate. they were sampled at weekly intervals in order to determine the duration of colonisation. the significance of potential pathogens was assessed. vascular ulcers were of low mean wound grade (1.87), short duration (1.58 weeks) and potential pathoge ...19873117891
imipenem: the first thienamycin antibiotic.imipenem is a new beta-lactam which differs chemically from penicillins and cephalosporins but has a similar mode of action. it is a potent antibacterial agent with a broad spectrum of activity encompassing both aerobes and anaerobes. however, in vitro, it does not appear to offer a great improvement over other available agents for the treatment of infections caused by problem organisms such as pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus and enterococci. the results of cl ...19863118345
[occurrence and incidence of bacteria in the area of drinking water with antagonistic relations to indicator bacteria].during a period of 8 months about 3000 drinking water samples derived from different water works and distribution systems around the area of the city bonn were examined for colony count, e. coli and coliform bacteria and bacteria suppressing the growth of e. coli and s. faecalis. a modified agar diffusion test was used for the determination of antagonistic substances. bacteria which revealed an antagonistic activity were isolated and identified. the majority of water samples (81.9% of the sample ...19873118613
[ability to survive and interactions of selected bacterial species in activated carbon filters].the survival and the interactions of selected, hygienically relevant bacterial species in activated carbon filters was investigated. the numbers of escherichia coli, pseudomonas aeruginosa and p. putida increased in previously sterilized filters. after inoculation all bacteria (10(6] were adsorbed. during a period of 6-10 days the filter effluent contained no detectable bacteria. after that the colony numbers increased to a constant level of 10(4)-10(5) colony forming units (cfu)/ml effluent and ...19873118614
location of antibiotic resistance markers in clinical isolates of enterococcus faecalis with similar antibiotypes.eight wild-type strains of enterococcus faecalis, resistant to chloramphenicol (cmr), erythromycin (emr), tetracycline (tcr), and minocycline (mnr), were examined for the genetic basis of their antibiotic resistance, five of the strains transferred all of their antibiotic resistance markers by conjugation, while the other three strains transferred only tcr and mnr. cmr and emr determinants were localized by dna-dna hybridization experiments, in which the cmr gene of plasmid pip501, of group b st ...19873118797
endodontic treatment of experimentally induced apical periodontitis in dogs. 19873119322
a comparison of the in-vitro activity of seventeen antibiotics against streptococcus faecalis.the in-vitro activity of seventeen antibiotics against 177 clinical strains of streptococcus faecalis was evaluated. ampicillin and mezlocillin were the most active beta-lactams tested (geometric mean mics, 0.61 and 0.74 mg/l, respectively). aminoglycosides showed poor activity and 22.6% of the strains were resistant to 2000 mg/l of streptomycin. no correlation was observed between mics of gentamicin and streptomycin. moderate activity was observed when testing norfloxacin and ciprofloxacin. tei ...19873119551
regulation of arginine-ornithine exchange and the arginine deiminase pathway in streptococcus lactis.streptococcus lactis metabolizes arginine by the arginine deiminase (adi) pathway. resting cells of s. lactis grown in the presence of galactose and arginine maintain a high intracellular ornithine pool in the absence of arginine and other exogenous energy sources. addition of arginine results in a rapid release of ornithine concomitant with the uptake of arginine. subsequent arginine metabolism results intracellularly in high citrulline and low ornithine pools. arginine-ornithine exchange was s ...19873119567
role of immunoblotting in the diagnosis of culture negative and enterococcal endocarditis.serum samples from patients with endocarditis and septicaemia due to enterococcus faecalis, enterococcus faecium, streptococcus bovis, and streptococcus sanguis were immunoblotted against antigenic extracts from all four species. in e faecalis endocarditis there was a strong igm response to e faecalis antigenic bands of 112, 88-90, and 45-47 kd and a strong igg response to 88-90 and 45-47 kd bands. in e faecium endocarditis there was a pronounced igg response to an e faecium band of 82-90 kd. fo ...19873119674
the diagnosis and management of urinary tract infection in children: a two year study in army general practice.a study of possible new cases of urinary tract infection in a population of some two thousand children under the age of twelve years is described. over a two year period one hundred and four children were seen in the study and seventeen were found to have a significant bacterial infection. presenting symptoms, urinalysis results, investigation and methods of follow-up are examined. the results suggest that the incidence of detectable urinary system abnormality in children presenting for the firs ...19873119825
an anterior limbal approach to vitrectomy for the surgical management of infectious endophthalmitis.we present two cases illustrating a surgical approach to the diagnosis and treatment of infectious endophthalmitis following extracapsular cataract extraction with insertion of a posterior chamber lens. in both cases, a vitrectomy was initiated through a limbal approach after clearing the anterior chamber, thus allowing improved visualization of the vitreous cavity. the vitrectomy probe was then passed through a peripheral iridotomy, the peripheral posterior capsule and/or zonules, and into the ...19873120072
[comparative evaluation of the antibacterial activity of nitrofuran derivatives]. 19873120163
quality control limits for microdilution susceptibility tests with aztreonam, imipenem, ceftriaxone, ceftazidime, ceftizoxime, cefuroxime, and cefonicid.a six-laboratory collaborative study was performed in order to define quality control limits for microdilution tests with seven new beta-lactams (aztreonam, imipenem, ceftriaxone, ceftazidime, ceftizoxime, cefuroxime, and cefonicid). four standard control strains were tested and the expected mic limits for each of the appropriate drug-microorganism combinations were defined.19873121242
disk diffusion susceptibility testing and broth microdilution quality control guidelines for bmy-28100, a new orally administered cephalosporin.the bmy-28100 30-micrograms-disk test was evaluated by using 615 clinical isolates. regression analyses and error rates were determined, leading to the recommendation of greater than or equal to 18-mm zone diameters (mic correlate, greater than or equal to 8.0 micrograms/ml) for susceptibility and less than or equal to 14-mm zone diameters (mic correlate, greater than or equal to 32 micrograms/ml) for resistance. nearly all false-susceptible disk test results were among the providencia spp. and ...19873121667
enumeration of pathogenic bacteria from sewage sludge in kuwait.domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ...19873121985
neonatal enterococcal sepsis: case-control study and description of an outbreak.an increased incidence of neonatal sepsis caused by group d streptococci, specifically enterococci (gde), during a recent 6-month period prompted a 5-year review of enterococcal sepsis in our neonatal intensive care unit. sixteen episodes occurred in 14 babies. gde accounted for 8 of 19 (42%) episodes of neonatal bacteremia during the epidemic period vs. only 8 of 159 (5%) episodes during the remaining 4.5 years (endemic period) (p less than 0.001). blood isolates were all identified as streptoc ...19873122158
effect of streptococcus faecalis on the growth of bacteroides species and anaerobic cocci in mixed infection.the relationship between streptococcus faecalis and anaerobic bacteria was studied in a subcutaneous abscess model in mice. included in the study were one strain of s. faecalis, three strains each of the bacteroides fragilis group and b. melaninogenicus group, and five strains of anaerobic cocci. the relationship between the organisms was studied by determining the individual change in colony-forming units (cfus) of the two bacterial components that induces abscesses in mice, compared to the cfu ...19883122348
mucosal and serum immunity following commensal enteric colonization. 19873122537
production of staphylococcal enterotoxin in mixed cultures.two staphylococcus aureus strains were grown in brain-heart infusion (bhi) broth and a meat medium with bacillus cereus, streptococcus faecalis, escherichia coli, and pseudomonas aeruginosa. both s. aureus strains grew well and produced enterotoxin in the presence of s. faecalis in bhi broth; however, enterotoxin production was observable in the meat medium only when the s. aureus inoculum was greater than the s. faecalis inoculum. s. aureus fri-100 grown with b. cereus produced enterotoxin in b ...19873122653
conjugative mobilization as an alternative vector delivery system for lactic streptococci.due to the current variability in applying polyethylene glycol-mediated protoplast transformation to lactic streptococci, a study was undertaken to assess the feasibility of conjugative mobilization as an alternative method for vector delivery. by using the broad-host-range conjugative plasmid pva797, the partially homologous cloning vector pva838 was successfully introduced into various strains of streptococcus lactis, streptococcus cremoris, streptococcus lactis subsp. diacetylactis, streptoco ...19873122654
conjugal transfer of plasmid pam beta 1 in lactobacillus reuteri and between lactobacilli and enterococcus faecalis.the broad-host-range plasmid pam beta 1 (erythromycin resistance) was transferred conjugally from streptococcus lactis to lactobacillus reuteri, l. murinus, and l. fermentum. transfer of pam beta 1 between two l. reuteri strains occurred, and lactobacillus transconjugants could act as donors of pam beta 1 in crosses with enterococcus faecalis jh2-2.19873122658
assessment of antiseptic bladder washout procedures using a physical model of the catheterised bladder.a simple physical model of the catheterised bladder has been used to assess the activities of antiseptic agents that have been recommended as bladder instillations in the treatment of urinary tract infections in patients with indwelling catheters. the activities of povidone-iodine, phenoxyethanol, chlorhexidine, chlorhexidine supplemented with edta + tris, noxythiolin and neomycin were examined against selected species of uropathogens. except for phenoxyethanol against pv. stuartii and possibly ...19873122868
the functions of autolysins in the growth and division of bacillus subtilis.some bacteria, such as streptococci, exhibit growth from discrete and well-defined zones. in streptococcus faecalis, growth zones can be observed in the electron microscope, and the position of the zone can be used as a marker for cell cycle events. growth of the cell surface of bacillus subtilis appears to be by a much different mechanism from that of streptococci. cell elongation takes place by the insertion at many sites in the cell cylinder of peptidoglycan components. the insertion occurs o ...19873123142
single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci.growth in a single broth microdilution well containing gentamicin at a concentration of 500 micrograms/ml predicted high-level resistance to gentamicin (mic, greater than or equal to 2,000 micrograms/ml) in 505 of 508 clinical isolates of enterococci. failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 micrograms of gentamicin per ml. we recommend this procedure be adop ...19873123515
trimethoprim-sulfamethoxazole in cyst fluid from autosomal dominant polycystic kidneys.cyst infection in patients with autosomal-dominant polycystic kidney disease (adpkd) is often refractory to therapy, in part because of the limited entry of commonly used antibiotics into cyst fluid. to study the efficacy of trimethoprim-sulfamethoxazole in cyst infection, cyst fluid was obtained by percutaneous aspiration or at surgery from eight patients with adpkd receiving trimethoprim-sulfamethoxazole. cysts were categorized as nongradient or gradient by cyst-fluid sodium concentration. tri ...19873123761
kinetic mechanism and nucleotide specificity of nadh peroxidase.nadh peroxidase is a flavoprotein isolated from streptococcus faecalis which catalyzes the pyridine nucleotide-dependent reduction of hydrogen peroxide to water. initial velocity, product, and dead-end inhibition studies have been performed at ph 7.5 and support a ping-pong kinetic mechanism. in the absence of hydrogen peroxide, both transhydrogenation between nadh and thionad, and isotope exchange between [14c]nadh and nad, have been demonstrated, although in both these experiments, the maximal ...19883124762
enterococcal bacteremia in a medical intensive care unit.we reviewed retrospectively from 1982 through 1984 35 cases of enterococcal bacteremia in a medical icu. of these, 27 patients acquired nosocomial infections; their mean previous hospital stay was 17 +/- 4 days. thirteen had a serious debilitating disease; 20 received previous antibiotic therapy. the infection focus was not found in 16 patients. the deaths of nine patients were related directly to enterococcal bacteremia. mortality was significantly lower in patients with nosocomial infections, ...19883125003
acute bacterial endocarditis on a normal aortic valve following vaginal delivery.we present a case of post-partum bacterial endocarditis on a normal aortic valve. infection occurred following vaginal delivery. this complication, due to group d streptococcus faecalis, has not been previously described in this context.19883125116
effects of medium and inoculum variations on screening for high-level aminoglycoside resistance in enterococcus faecalis.enterococcus faecalis isolates that are refractory to aminoglycoside-penicillin synergy can be detected by their ability to grow in the presence of high concentrations of aminoglycoside (2,000 micrograms/ml). in past studies investigators have used a variety of media and inoculum sizes to perform high-level aminoglycoside resistance screens, but little is known about how these variations affect test accuracy. we screened 63 e. faecalis strains on different media by using various inoculum sizes a ...19883125217
high-content aminoglycoside disks for determining aminoglycoside-penicillin synergy against enterococcus faecalis.we investigated the use of high-content aminoglycoside disks for determining enterococcus faecalis susceptibility to aminoglycoside-penicillin synergy. the susceptibility of the organisms to synergy was established by 24-h time-kill studies performed with streptomycin, kanamycin, amikacin, gentamicin, and tobramycin, alone and in combination with penicillin. a total of 20 isolates that were susceptible to all drug combinations and 20 strains that were resistant to each aminoglycoside-penicillin ...19883125218
[a case of spontaneous bacterial peritonitis in liver cirrhosis with idiopathic pneumoperitoneum]. 19873125368
combined use of phage typing, enterococcinotyping and species differentiation of group d streptococci as an effective epidemiological tool.ninety-five group d streptococcal isolates from the feces of 95 healthy persons were compared with 157 group d streptococcal isolates from 38 patients of the surgical intensive care unit (sicu). the typing systems consisted of phage typing, enterococcinotyping and species differentiation. strains isolated from fecal specimens showed high individuality (66 combination types) whereas strains from the sicu revealed strongly uniform types (32 combination types, three types comprised 83 isolates, i.e ...19873125708
comparison of the post-antibiotic effect of streptococcus faecalis and streptococcus faecium with ampicillin alone or combined with streptomycin: studies on a novel type of antimicrobial interaction.determination of post-antibiotic effect (pae) and time-kill studies were made with twelve strains of streptococcus faecalis and nine strains of streptococcus faecium, comparing the effect of ampicillin alone with a combination of ampicillin and streptomycin at achievable serum concentrations. bactericidal synergism (greater than or equal to one log10 decrease in viable counts) and prolongation in pae (greater than or equal to 0.5 h) were demonstrated in all streptomycin-susceptible strains (mini ...19873125717
enterococcal endocarditis: recent experience. 19883125734
characterization and comparison of two penicillinase-producing strains of streptococcus (enterococcus) faecalis.we identified two beta-lactamase-positive enterococci. one strain was high-level (mic, greater than 2,000 microgram/ml) gentamicin resistant; the other was not (mic, 12.5 microgram/ml). beta-lactamase production was extrachromosomally mediated in both strains, and both strains showed an inoculum effect reversed by beta-lactamase inhibitors. the strain lacking high-level gentamicin resistance showed synergistic killing with a combination of penicillin, clavulanic acid, and gentamicin.19883126703
responses of chickens to streptococcus faecalis: genotype-housing interactions.hens from lines selected for high and low juvenile body weight were maintained in single-bird wire cages or in litter-floor flocks and inoculated intravenously with streptococcus faecalis. responses to the inoculation, as measured by changes in body weight, plasma xanthophyll levels, heterophil/lymphocyte ratios, and mortality, were more severe in the high-body-weight line than the low-body-weight line. the effect of cage vs. floor housing varied according to genetic line. responses of individua ...19873126728
preliminary experience with ceftazidime monotherapy in perinatal infection.ceftazidime, a new cephalosporin antibiotic, was used as monotherapy to treat 75 term neonates with suspected perinatal bacterial infection. septicemia was documented in ten patients (13%). ceftazidime was administered as 25 mg/kg doses intravenously every 12 h. peak serum concentrations at 0.5 h after dose ranged from 57 to 115 micrograms/ml and trough serum concentrations prior to next dose from 2 to 15 micrograms/ml. the overall clinical, bacteriological and laboratory response to ceftazidime ...19873127359
contribution of the microflora to proteolysis in the human large intestine.protease activities in human ileal effluent were approximately 20-fold greater than in normal faeces. comparative studies with faeces from a person who did not have a pancreas suggested that a substantial proportion of the proteolytic activity in normal faeces was of bacterial origin. thimerosal, iodoacetate, edta and cysteine significantly inhibited proteolysis in faeces, but not in small intestinal contents, showing that cysteine and metalloproteases were produced by bacteria in the large gut. ...19883127369
the effect of growth-promoting antibiotics on the faecal enterococci of healthy young chickens.small groups of chickens were given feed containing either avoparcin, nitrovin, virginiamycin or zinc bacitracin from the day of their purchase as day-olds. differences between the birds receiving growth promoters and the untreated controls were observed during the last third of the 23 d survey period. the enterococcal population of the 'dosed' birds contained a greater proportion of enterococcus faecium than did that of the control birds while the converse was true for ent. gallinarum. this app ...19883127371
effects of bacterial infection on absorbable vascular ligating clips.electron microscopic observations of two implanted absorbable clips, absolok and lactomer, infected with escherichia coli and streptococcus faecalis, were carried out. an overgrowth of bacteria was observed over the entire surface of the clips. the mating surfaces of the lactomer clips and the locking mechanisms of both types of clip were more heavily infected--that is, provided better support for bacterial growth--than other areas of the clips in the initial postimplantation period. however, wi ...19883127583
a shift from acute to chronic spontaneous pyelonephritis in male mm mice associated with a change in the causal micro-organisms.proteus mirabilis was the predominant cause of acute diabetes-associated pyelonephritis occurring spontaneously in male mm mice until they were segregated in a new environment. thereafter pasteurella pneumotropica and streptococcus faecalis emerged collectively as the most common causal organisms, the pyelonephritis became more chronic and proteus mirabilis isolates from faeces and urine produced atypical non-swarming colonies on blood agar plates. this did not account for the reduced pathogenic ...19883127633
constitutive expression of erythromycin resistance mediated by the ermam determinant of plasmid pam beta 1 results from deletion of 5' leader peptide sequences.we have sequenced the erythromycin resistance determinant (erm) of the streptococcus faecalis plasmid pam beta 1 to investigate its relationship to other known resistance determinants. we show that this determinant is strongly (99%) homologous at the dna level to that of plasmid pam77 (streptococcus sanguis) and of transposon tn917 (s. faecalis). moreover, nucleotide sequence comparison with the determinants of pam77 and tn917 shows that most of the probable regulatory region is absent, providin ...19873127839
enterococcal superinfection in patients treated with ciprofloxacin.two patients developed serious enterococcal superinfection following therapy with intravenous ciprofloxacin. the strains causing bacteraemia were susceptible to low concentrations of ciprofloxacin when tested at a standard inoculum. however, at an inoculum of 1 x 10(7) cfu/ml they were resistant to high concentrations of ciprofloxacin. our data suggest that ciprofloxacin should not be used to treat patients with serious enterococcal infection and that patients treated with ciprofloxacin should b ...19883128516
comparison of 1% silver sulfadiazine with and without 1% chlorhexidine digluconate for topical antibacterial effect in the burnt infected rat.the addition of 1% chlorhexidine digluconate to 1% silver sulfadiazine cream (cdss) was compared with 1% silver sulfadiazine (ss) alone to assess the antibacterial effect of a once-daily application of the therapies on an experimental rat model with a 20% full-thickness burn wound seeded with 10(8) microorganisms originally isolated from infected wounds of burn patients. separate series evaluated staphylococcus aureus, pseudomonas aeruginosa, enterobacter cloacae, and streptococcus faecalis. the ...19883128553
evaluation of the cobas bact automated system for susceptibility testing of enterobacteriaceae, pseudomonas aeruginosa, and enterococcus faecalis to azlocillin, mezlocillin, and ciprofloxacin compared to nccls and din standards.the aim of automated susceptibility testing systems like the cobas bact is to provide the clinicians with rapid and reliable results for the care of patients and to decrease the work load in microbiological laboratories. because data about accuracy on mezlocillin, azlocillin and ciprofloxacin were lacking, we investigated 184 bacterial strains and compared the results of the cobas bact susceptibility testing to standardized agar dilution and agar diffusion methods. essential correlations for all ...19883129374
an open study of the safety and efficacy of enoxacin in complicated urinary tract infections.thirty-one patients with serious or complicated urinary tract infections were treated with oral enoxacin for between four and eight days. twenty-five patients with microbiologically confirmed infections completed treatment with 400 mg enoxacin twice daily and at the end of treatment all urine cultures were negative. at short term follow-up, five to nine days after therapy, 21 of the 25 patients were still infection free. in total, reinfection or relapse occurred in 12 patients during the four to ...19883129393
[comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1985). ii. background of patients].we have investigated relationships between patients' backgrounds and isolates from urinary tract infections in reference to various conditions, such as sexes, age distribution by sex, species of causative organisms by sex, age distribution according to species and types of infections, species and infections before and after treatment with antibacterial agents, and species and detected places. as for the relationship between sexes and types of infections, although simple urinary tract infections ...19873129602
[the effects of cefoperazone on the intestinal bacterial flora and the incidence of diarrhea in children]. 19873130444
characterization of the 1-phosphohistidinyl residue in the phosphocarrier protein hpr of the phosphoenolpyruvate: sugar phosphotransferase system of streptococcus faecalis.the phosphocarrier protein hpr of the bacterial phosphoenolpyruvate:sugar phosphotransferase system contains 1-phosphohistidine at residue 15. this residue and the active site residue arg-17 are conserved in hprs isolated from both gram-positive and -negative bacteria. the ph- and temperature-dependent hydrolysis of the 1-phosphohistidinyl residue in p-hpr from streptococcus faecalis has been investigated. the results show that the hydrolysis properties are very similar to those previously repor ...19883130884
high-performance liquid chromatographic analysis of demethylmenaquinone and menaquinone mixtures from bacteria.demethylmenaquinone and menaquinone mixtures from some species of enterobacteria were analysed by reverse-phase partition high-performance liquid chromatography. this method allowed clear separation and quantitative determination of these quinone components.19883131293
[microbiological aspects of urinary infections in hospitalized and ambulatory elderly patients].the authors studied the microorganisms found in urocultures of three groups of elderly patients giving an exact map of the microbiology and epidemiology of the urinary tract infections of elderly patients. they shown also the calibrated loop seeding method as the most efficient to evaluate low bacterial concentration in urines which in these patients are significant.19873131829
establishment of a typing system for group d streptococci.group d streptococci (n = 971, 4037 samples, 139 patients) were isolated from two intensive care units. differentiation and typing revealed 6 species, 25 phage types, and 85 enterococcinotypes. distribution of species showed strong differences especially concerning enterococcus faecium which was rarely isolated from extraintestinal sites (5.9%). phage typing was species specific and discriminated enterococcus faecalis (n = 703 typable isolates, 19 phage types) and the other group d streptococci ...19883131979
plasmids and pheromone response of the beta-lactamase producer streptococcus (enterococcus) faecalis hh22.streptococcus (enterococcus) faecalis hh22 is a clinical isolate that produces beta-lactamase and is resistant to various other antimicrobial agents. in this study, hh22 was found to contain three conjugative plasmids and a conjugative transposon. pbem10 encodes beta-lactamase, gentamicin resistance, and a response to the peptide pheromone cad1; pam323 encodes erythromycin resistance; and pam324 encodes no known resistance. the latter two plasmids respond to pheromones designated cam323 and cam3 ...19883132094
conjugal transfer of group b streptococcal plasmids and comobilization of escherichia coli-streptococcus shuttle plasmids to lactobacillus plantarum.the antibiotic resistance group b streptococcal plasmids, pip501 and pva797, were conjugally transferred from streptococcus faecalis to lactobacillus plantarum. the escherichia coli-streptococcus shuttle plasmids, pva838 and psa3, were mobilized from s. sanguis to l. plantarum by pva797 via cointegrate formation. pva838 readily resolved from pva797 and was present in l. plantarum as deletion derivatives. the pva797::psa3 cointegrate failed to resolve in l. plantarum.19883132101
evidence for a single active-site cysteinyl residue in the streptococcal nadh peroxidase.substrate reduction of the streptococcal flavoprotein nadh peroxidase, followed by anaerobic denaturation and titration with 5,5'-dithiobis(2-nitrobenzoate), yields a stoichiometry of one protein thiol per mole of fad. analysis of the nadh peroxidase, purified from cultures of streptococcus faecalis 10cl grown on a chemically-defined medium containing [35s]cysteine, confirms the stoichiometry of one cysteinyl residue per subunit and allows the isolation and sequencing of the corresponding cystei ...19883132163
synergistic effect of ampicillin or vancomycin in combination with decreasing concentrations of streptomycin against enterococci.the extent of synergistic killing and duration of the post-antibiotic effect (pae) was evaluated in streptomycin-susceptible strains of streptococcus faecalis and streptococcus faecium with 20 mcg/ml of ampicillin or 10 mcg/ml of vancomycin in combination with decreasing concentrations of streptomycin (20, 10, 5, 2, 1, 0.5 mcg/ml). the bactericidal activity or duration in recovery period declined progressively in a linear pattern with decreasing concentrations of streptomycin. one log10 or great ...19883132184
group d streptococcal neonatal mastitis. 19883132696
prior experience and response of chickens to a streptococcal infection.streptococcus fecalis infection was diagnosed in a population of individually caged meat-type pullets. a retrospective analysis showed that the infection occurred in pullets fed ad libitum and not in those whose feed intake was restricted. among the former, resistance as measured by changes in body weight and blood heterophils and lymphocytes was greater for those from which blood samples had been obtained than from those which had not been bled. these data are consistent with the hypothesis tha ...19873132704
[resistance to fosfomycin trometamol (monuril): relation to bacterial virulence and the significance of "in" colonies]. 19873132876
microbial spoilage of pre-cooked potato-topped pies.the ecological succession of bacteria which developed in pre-cooked potato-topped pies stored at two different temperatures was examined. bacillus, streptococcus and staphylococcus-micrococcus spp. were the predominant organisms isolated from freshly prepared pies and those stored at 4 degrees and 37 degrees c. none of these groups of bacteria caused significant biodeterioration of pies held at 4 degrees c, but all groups grew well in pies stored at 37 degrees c and achieved counts of ca 10(8)/g ...19883133345
resistance patterns of important gram-positive pathogens.staphylococcus aureus, streptococcus pneumoniae and enterococci are among the most important gram-positive organisms responsible for serious infections in hospitals as well as in the community. we report here the resistance patterns encountered in these species at st joseph and broussais hospitals in paris.19883133348
transformation of bacteria with plasmid dna by electroporation.the possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (enterococcus faecalis) and two gram-negative bacteria (escherichia coli and pseudomonas putida) with plasmid dna was investigated. e. faecalis protoplasts could be transformed by electroporation with a transformation frequency of 10(4) to 10(5) transformants/micrograms plasmid. untreated--i.e., washed--cells of e. coli could be transformed with rates of 1 x 10(5) transformants/micrograms p ...19883133958
the response of streptococcus faecalis to ciprofloxacin, norfloxacin and enoxacin.the response to three quinolones of a clinical isolate of streptococcus faecalis was studied by continuous turbidimetric monitoring, by viable counting, and in an in-vitro model of the treatment of bacterial cystitis. dense populations (c. 8 x 10(6) cfu/ml) responded to ciprofloxacin, norfloxacin and enoxacin at concentrations below the conventionally determined mic, but variants, shown to exhibit decreased susceptibility, emerged during overnight incubation. the variants were cross-resistant to ...19883134318
gentamicin resistance plasmids of enterococci from diverse geographic areas are heterogeneous. 19883134493
enterococcal bacteremia: clinical features, the risk of endocarditis, and management.the enterococci, members of the group d streptococci and the predominant aerobic streptococci of the gastrointestinal and female genital tracts, have long been recognized as significant pathogens in infective endocarditis. over the past 2 decades, enterococci have become increasingly important nosocomial pathogens, related to their intrinsic resistance to many antibiotics, especially the cephalosporins, and the greatly increased use of antimicrobial therapy in hospitals. recent reports have docu ...19883134590
thromboxane-associated pulmonary hypertension during three types of gram-positive bacteremia in piglets.thromboxane-associated pulmonary hypertension occurs in animals during intravenous infusion of group b streptococcus (gbs), a gram-positive neonatal pathogen. we postulated that other gram-positive neonatal pathogens, such as streptococcus fecalis (ent) and staphylococcus epidermidis (s. epi) would also induce increased thromboxane synthesis and pulmonary hypertension when infused into piglets. we observed similar hemodynamic and gas exchange abnormalities during stepwise increases in the dose o ...19883134642
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