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release of romanomermis iyengari (nematoda:mermithidae) to control aedes taeniorhynchus (diptera:culicidae) in punta del este, isla de la juventud, cuba.preparasites of romanomermis iyengari welch were applied at the rate of 1,000 preparasites per square meter of surface area in 5 natural breeding sites in carbonate rock depressions to control aedes taeniorhynchus wiedeman larvae. larval parasitism averaged 100, 97, 81, and 80% in 1-4 instars, respectively. three of 5 sites produced parasitism in excess of 90%. the range of salinity and water depth had little influence on the levels of parasitism. ae. taeniorhynchus density was reduced from 78 t ...19968699466
short report: lack of virus replication in arthropods after intrathoracic inoculation of ebola reston virus.to evaluate the potential for arthropods to serve as reservoir hosts of ebola virus, three mosquito species, aedes albopictus, aedes taeniorhynchus, and culex pipiens, and a soft tick, ornithodoros sonrai, were inoculated with 1o2.5 plaque-forming units of ebola reston virus. after incubation at 22 degrees c for 11 days, at least six specimens of each species were triturated and examined for evidence of viral replication by enzyme-linked immunosorbent assay and plaque assay. there was no evidenc ...19968702028
field evaluation of heat as an added attractant to traps baited with carbon dioxide and octenol for aedes taeniorhynchus.collection size of 3 species of mosquitoes (aedes taeniorhynchus, anopheles atropos, and culex nigripalpus) and a species of biting midge (culicoides furens) in cdc-type traps baited with carbon dioxide and octenol were significantly increased with the addition of heat. the presence of heat also significantly reduced collection size of the tabanid diachlorus ferrugatus.19958825507
influence of ground ulv droplet spectra on adulticide efficacy for aedes taeniorhynchus.a series of 76 field trials in a vegetated habitat with caged adult aedes taeniorhyncus mosquitoes determined that ground ultra-low volume treatment efficiency was significantly influenced by droplet size. small droplets (7-microns volume median diameter [vmd]) produced the lowest mosquito mortality at all test distances (100-500 ft.). a vmd of 26 microns was effective at 100-300 ft., but was not effectual at 400-500 ft. droplets in the 15-microns range were the most effective overall, at the di ...19968827622
construction of a field trap for initiating an ovipositional response in aedes taeniorhynchus.an oviposition trap was constructed for the black saltmarsh mosquito, aedes taeniorhynchus. the trap consisted of a 50 x 60-cm piece of contaminated 100% cotton bath towel, saturated with 85% tap water, a container, and a cover of dried plant parts placed over the contaminated toweling. this combination initiated oviposition. contamination of the toweling was due to populations of bacteria and fungi. the eggs recovered were free from soil and debris.19968887231
a larval mosquito survey in northeastern arkansas including a new record for aedes albopictus.an extensive larval mosquito survey was conducted in craighead county in northeastern arkansas in 1990-91. nineteen species representing 8 genera were collected. culex restuans was the most cosmopolitan species in relation to habitat utilization, being collected in 5 or 7 habitat types sampled. a major range extension of aedes taeniorhynchus in arkansas was documented as well as the first known collection of aedes albopictus in the northeastern part of the state.19948965075
acoustical detection of aedes taeniorhynchus swarms and emergence exoduses in remote salt marshes.swarms and emergency exoduses of aedes taeniorhynchus mosquitoes produce sounds detectable from 10 to 50 m in a quiet environment. background noise levels as low as 21 db (decibels referenced to 20 micropa) are present at dusk between frequencies of 0.3 and 3.4 khz. a mosquito swarm with a sound pressure level of 25-35 db is detectable over tens of meters in the marsh, if not in the 40-60-db background noise of a typical urban environment. individually caged ae. taeniorhynchus also are detectabl ...19948965083
epidemic venezuelan equine encephalitis in la guajira, colombia, 1995.in 1995, the first venezuelan equine encephalitis (vee) outbreak in colombia in 22 years caused an estimated 75,000 human cases, 3000 with neurologic complications and 300 fatal, in la guajira state. of the state's estimated 50,000 equines, 8% may have died. an epizootic ic virus, probably introduced from venezuela, was rapidly amplified among unvaccinated equines. record high rainfall, producing high densities of vector aedes taeniorhynchus, led to extensive epidemic transmission (30% attack ra ...19979086137
hemagglutinins in mosquitoes and their role in the immune response to brugia malayi (filarioidea:nematoda) larvae.hemagglutinins were determined in six species of mosquitoes that are susceptible and refractory to brugia malayi (filarioidea: nematoda). high titers of hemagglutinins were found in the salivary gland extract and in the body fluid of a completely refractory species, aedes taeniorhynchus, and in partially refractory species, anopheles quadrimculatus but low levels of hemagglutinins were also present in the body fluid of aedes aegypti (black-eye, liverpool strain), a susceptible species. hemagglut ...19979505436
failure to predict abundance of saltmarsh mosquitoes aedes sollicitans and a. taeniorhynchus (diptera: culicidae) by using variables of tide and weather.weather and tide variables were used to develop regression models with light trap counts of aedes sollicitans (walker) and aedes taeniorhynchus (wiedemann) from 1984 to 1995. rains, tides, and temperatures, both during and preceding the sample period, all showed significant correlations with trap counts. these multiple regression models forecasted general population levels during 1996 and 1997, but not the size of the peaks. therefore, weather alone can predict general trends but cannot be used ...19989615534
bva 2 mosquito larvicide--a new surface oil larvicide for mosquito control.bva 2 mosquito larvicide was evaluated in laboratory pan tests against 3rd-instar aedes taeniorhynchus (wied.), culex quinquefasciatus say, and culex nigripalpus theobald larvae. bva 2 was as effective as the standard, gb-1111, at 14 liters/ha (> 99.1% vs. 99.8%). in small field plot tests bva 2 mosquito larvicide applied at 28 liters/ha was as effective as gb-1111 (99.0% vs. 99.8%) 24 h posttreatment. operationally, applied by helicopter at 46.8 liters/ha, bva 2 mosquito larvicide was more effe ...19989673922
mosquito (aedes taeniorhynchus) resistance to methoprene in an isolated habitat.salt-marsh mosquitoes (aedes taeniorhynchus), collected on 2 barrier islands in lee county, florida, that had been treated from 1989 to 1994 with 150-day methoprene briquets, were bioassayed with technical s-methoprene in the laboratory. susceptibility of the indigenous captiva strain (median lethal concentration [lc50] estimate, 6.71 ppb) collected from captiva island was 14.9-fold lower than the naive flamingo strain (lc50 estimate, 0.45 ppb) from everglades national park. the lover's key stra ...19989673923
mosquito frequency and feeding habits in an enzootic canine dirofilariasis area in niterói, state of rio de janeiro, brazil.heartworm, a chronic fatal mosquito-borne canine disease, is frequently diagnosed in dogs from the state of rio de janeiro, where its prevalence is 29.7% in the city of niterói. nevertheless it is rarely detected in cats (0.8%) from the same state. dogs are the primary source of infection to mosquitoes. because cats either do not demonstrate microfilaremia or it is too low and transient for transmission. a mosquito survey was conducted in itacoatiara, niterói, from march 1995 to february 1996, u ...19989698883
osmotic effects as a factor modifying insecticide toxicity on aedes and artemia.euryhaline species are more tolerant of various insecticides under isosmotic conditions. two euryhaline species, aedes taeniorhynchus (wiedemann) and artemia sp., were exposed to four insecticides (aldicarb, dimethoate, imidacloprid, tebufenozide), under isosmotic and hyperosmotic conditions. mortality under these two osmotic conditions was observed and compared to evaluate salinity as a contributing factor to insecticide toxicity. artemia was more tolerant of all chemicals tested than a. taenio ...19989756708
effect of salt concentration in larval rearing water on susceptibility of aedes mosquitoes (diptera: culicidae) to eastern equine and venezuelan equine encephalitis viruses.the effect of salt concentration in larval rearing water on the susceptibility of adult aedes taeniorhynchus (wiedemann) and aedes sollicitans (skuse) to infection with eastern equine encephalomyelitis (eee) virus was tested in the laboratory. ae. sollicitans was more susceptible to infection (79%, n = 82) and viral dissemination (16%) with eee virus than was ae. taeniorhynchus (42%, n = 184) and (5%), respectively, when fed on a chick with a viremia of 10(7) +/- 0.1 plaque-forming units/ml; how ...19989775590
evaluation of attractant-baited traps/targets for mosquito management on key island, florida, usa.a three-year research project was conducted on key island, collier county, florida, usa, to evaluate an innovative attractant-based mosquito management technique. in the first year, species composition, relative abundance, and spatial distribution were determined. although 16 species were collected, the dominant species was the black salt march mosquito, aedes taeniorhynchus, which was the primary pest species. efficacy of a single line barrier, consisting of 52 carbon dioxide (200 cc/min) + oct ...19989879073
laboratory evaluation of biotic and abiotic factors that may influence larvicidal activity of bacillus thuringiensis serovar. israelensis against two florida mosquito species.a technical powder of bacillus thuringiensis serovar. israelensis (b.t.i.) (vectobac tp, 5,000 international toxic units [itu]/mg), an aqueous suspension (vectobac 12as, 1,200 itu/mg), and a granular formulation (vectobac cg, 200 itu/mg) were tested in the laboratory under different biotic and abiotic, conditions for efficacy against larvae of saltwater (aedes taeniorhynchus) and freshwater (culex nigripalpus) mosquitoes. second-, 3rd-, and 4th-instar larvae of cx. nigripalpus were 1.3-3-fold mo ...199910342266
responses of male and female mosquitoes to repellents in the world health organization insecticide irritability test system.a study was conducted to compare responses of male and female aedes aegypti (linn.) and aedes taeniorhynchus (wiedemann) to 9 olfactory repellents in the world health organization insecticide irritability test system. an irritant insecticide (permethrin) and a control were included for comparison. aedes aegypti exhibited significantly more takeoffs than ae. taeniorhynchus, and female mosquitoes exhibited significantly more takeoffs than males. permethrin induced significantly more takeoffs than ...199910342270
colonization of culex nigripalpus theobald (diptera: culicidae) by stimulation of mating using males of other mosquito species.culex nigripalpus theobald (diptera: culicidae) was recolonized successfully by cohabiting aedes taeniorhynchus males or males of other mosquito species starting in a large outdoor cage under natural light-dark cycles and temperatures and ending in a 1-ft.3 cage under artificial light-dark cycles at 24 degrees c without added stimulation.199910342273
limited potential for mosquito transmission of genetically engineered, live-attenuated venezuelan equine encephalitis virus vaccine candidates.in an attempt to improve the current live-attenuated vaccine (tc-83) for venezuelan equine encephalitis (vee), specific mutations associated with attenuation of vee virus in rodent models were identified. these mutations were inserted into full-length cdna clones of the trinidad donkey strain of vee virus by site-directed mutagenesis, and isogenic virus strains with these mutations were recovered after transfection of baby hamster kidney cells with infectious rna. we evaluated 10 of these strain ...199910403340
vector competence of three venezuelan mosquitoes (diptera: culicidae) for an epizootic ic strain of venezuelan equine encephalitis virus.experimental studies were undertaken to evaluate the vector competence of selected mosquito species [aedes taeniorhynchus (wiedemann), culex declarator dyar and knab, and mansonia titillans (walker)] from northwestern venezuela for the epizootic (ic) strain of venezuelan equine encephalitis (vee) virus that was responsible for the 1995 outbreak of vee in this area. ae. taeniorhynchus was highly susceptible to infection (94% of 35), and 89% had a disseminated infection. virus-exposed ae. taeniorh ...199910467764
comparison of two american biophysics mosquito traps: the professional and a new counterflow geometry trap.large cage and field studies were conducted to compare the efficacy of 2 american biophysics corporation mosquito traps, the standard professional (pro) trap and a new counterflow geometry (cfg) trap. the pro trap utilizes conventional downdraft technology and the cfg trap uses a patent-pending technology. in large cage studies, similarly baited cfg traps captured approximately 1.7 times as many laboratory-reared aedes taeniorhynchus as the pro trap. the cfg trap baited with co2 + octenol result ...199910480115
genetic reassortment of rift valley fever virus in nature.rift valley fever virus (rvfv), a phlebovirus of the bunyaviridae family, is an arthropod-borne virus which emerges periodically throughout africa, emphasizing that it poses a major threat for animal and human populations. to assess the genetic variability of rvfv, several isolates from diverse localities of africa were investigated by means of reverse transcription-pcr followed by direct sequencing of a region of the small (s), medium (m), and large (l) genomic segments. phylogenetic analysis s ...199910482570
production of chymotrypsin-resistant bacillus thuringiensis cry2aa1 delta-endotoxin by protein engineering.cleavage of the cry2aa1 protoxin (molecular mass, 63 kda) from bacillus thuringiensis by midgut juice of gypsy moth (lymantria dispar) larvae resulted in two major protein fragments: a 58-kda fragment which was highly toxic to the insect and a 49-kda fragment which was not toxic. in the midgut juice, the protoxin was processed into a 58-kda toxin within 1 min, but after digestion for 1 h, the 58-kda fragment was further cleaved within domain i, resulting in the protease-resistant 49-kda fragment ...199910508095
brugia malayi microfilariae (nematoda: filaridae) enhance the infectivity of venezuelan equine encephalitis virus to aedes mosquitoes (diptera: culicidae).we examined the potentially conflicting effects that microfilarial (mf) enhancement of viral infectivity and mf-induced mortality in mosquitoes have on the vectorial capacity of aedes aegypti (l.), aedes triseriatus (say), and aedes taeniorhynchus (wiedemann) for venezuelan equine encephalitis virus (vee) when mosquitoes feed on gerbils co-infected with brugia malayi (buckley). groups of mosquitoes were fed on gerbils that were either dually infected (vee plus b. malayi mf) or singly infected (v ...199910593077
efficacy of dibrom, trumpet, and scourge against four mosquito species in louisiana.adult mortality of anopheles quadrimaculatus, culex quinquefasciatus, and the aedes spp. complex (aedes sollicitans and aedes taeniorhynchus) was observed after aerial ultra-low volume (ulv) exposure to dibrom, trumpet, and scourge. dibrom was applied at 112 g active ingredient (ai)/ha, trumpet at 112 g ai/ha, and scourge at 1.96 g ai/ha. at all time intervals, dibrom and trumpet were significantly more effective against the aedes spp. complex than against an. quadrimaculatus and cx. quinquefasc ...199910612604
field efficacy and nontarget effects of the mosquito larvicides temephos, methoprene, and bacillus thuringiensis var. israelensis in florida mangrove swamps.we compared the efficacy and nontarget effects of temephos, bacillus thuringiensis var. israelensis (b.t.i.), and methoprene applied by helicopter to control mosquito larvae in mangrove swamps on sanibel island, fl, in may 1997. three sites per treatment and 3 untreated sites were used. temephos (abate) was applied at 37 ml/ha (43% active ingredient [ai]), b.t.i. granules (vectobac g) were applied at 5.606 kg/ha (200 international toxic units/mg), and methoprene (altosid all) was applied at 213 ...199910612606
vector competence of two species of mosquitoes (diptera: culicidae) from southern california for dirofilaria immitis (filariidea: onchocercidae).two species of mosquitoes reared to adults from field-collected larvae or egg rafts in or near los angeles county, ca, were exposed to dogs with known levels of dirofilaria immitis (leidy) microfilariae per 20 cubic millimeters of blood. aedes taeniorhynchus (wiedemann) readily blood fed on an infected dog ingested microfilariae but did not become infected. culiseta incidens (thomson) also fed on an infected dog became infected, and 10% of the microfilariae developed to the l3 stage. cs. inciden ...200010730504
phylogenetic relationships among eighteen neotropical culicini species.the subgeneric relationships among 8 generic and infrageneric taxa of the culicini tribe were examined by cladistic analysis based on 30 larval mouthpart characters (maxillae and mandibles) of 7 subgenera of culex l. and 1 of deinocerites theobald. we analyzed 18 ingroup species as well as deinocerites melanophylum dyar and knab as a sister group, and aedes taeniorhynchus wiedemann as an outgroup. a parsimony analysis using the nona program resulted in 2 trees each of 109 steps (consistency and ...200010901630
density and distribution of dirofilaria immitis (nematoda: filarioidea) third-stage larvae in aedes sollicitans and aedes taeniorhynchus (diptera: culicidae).numbers and the distribution of third-stage larvae (l3) were investigated in aedes sollicitans (walker) and aedes taeniorhynchus (wiedemann) female mosquitoes fed dirofilaria immitis (leidy) infectious-blood with densities of microfilariae (mf) ranging from approximately 7,100-43,400 mf/ml. at each microfilarial density, a maximum of 63-66 infective larvae were recovered from an ae. sollicitans alive on day 15 after infection. in comparison with ae. taeniorhynchus, ae. sollicitans averaged great ...200011004780
aircraft-mediated mosquito transport: new direct evidence.a case is reported of a capture of an aedes taeniorhynchus female mosquito in an aircraft cabin as it attempted to bloodfeed on one of the authors during a transoceanic flight from the usa to the iberian peninsula.200011198921
vector competence of north american mosquitoes (diptera: culicidae) for west nile virus.we evaluated the potential for several north american mosquito species to transmit the newly introduced west nile (wn) virus. mosquitoes collected in the new york city metropolitan area during the recent wn virus outbreak, at the assateague island wildlife refuge, va, or from established colonies were allowed to feed on chickens infected with wn virus isolated from a crow that died during the 1999 outbreak. these mosquitoes were tested approximately 2 wk later to determine infection, disseminati ...200111296813
in vivo and in vitro development of the protist helicosporidium sp.we describe the discovery and developmental features of a helicosporidium sp. isolated from the black fly simulium jonesi. morphologically, the helicosporidia are characterized by a distinct cyst stage that encloses three ovoid cells and a single elongate filamentous cell. bioassays have demonstrated that the cysts of this isolate infect various insect species, including the lepidopterans, helicoverpa zea, galleria mellonella, and manduca sexta, and the dipterans, musca domestica, aedes taeniorh ...200111456323
temperature effects on the dynamics of aedes albopictus (diptera: culicidae) populations in the laboratory.we investigated how constant temperatures of 22, 24, and 26 degrees c experienced across the full life cycle affected the dynamics of caged populations of aedes albopictus (skuse). all cages were equipped with plastic beakers that served as sites for oviposition and larval development. we measured the per capita daily mortality and emergence rates of the adults and size of adult females, and estimated the intrinsic rate of increase (r) and asymptotic density (k) for each caged population. popula ...011476335
positively charged amino acid substitutions in the e2 envelope glycoprotein are associated with the emergence of venezuelan equine encephalitis virus.epidemic-epizootic venezuelan equine encephalitis (vee) viruses (veev) have emerged repeatedly via convergent evolution from enzootic predecessors. however, previous sequence analyses have failed to identify common sets of nucleotide or amino acid substitutions associated with all emergence events. during 1993 and 1996, veev subtype ie epizootics occurred on the pacific coast of the states of chiapas and oaxaca in southern mexico. like other epizootic veev strains, when inoculated into guinea pi ...200211799167
survey of container-breeding mosquitoes from the florida keys, monroe county, florida.a survey of container-breeding mosquitoes was conducted on urban islands (big coppitt key, rockland key, key west, and stock island) and rural islands (big pine key, cudjoe key, little torch key, no name key, ramrod key, saddlebunch keys, sugarloaf key, and summerland key) within the florida keys. five mosquito species were collected: aedes aegypti, culex nigripalpus, cx. quinquefasciatus, cx. salinarius, and ochlerotatus taeniorhynchus. plastic buckets, trash cans, and discarded plastic contain ...200111804461
vector infection determinants of venezuelan equine encephalitis virus reside within the e2 envelope glycoprotein.epizootic subtype iab and ic venezuelan equine encephalitis viruses (veev) readily infect the epizootic mosquito vector aedes taeniorhynchus. the inability of enzootic subtype ie viruses to infect this mosquito species provides a model system for the identification of natural viral determinants of vector infectivity. to map mosquito infection determinants, reciprocal chimeric viruses generated from epizootic subtype iab and enzootic ie veev were tested for mosquito infectivity. chimeras containi ...200212021373
laboratory bioassay studies to determine methoprene susceptibility in a natural population of ochlerotatus taeniorhynchus from the florida keys.larvae of florida keys ochlerotatus taeniorhynchus collected from no name key were colonized and evaluated against technical s-methoprene in laboratory beaker tests. glassware was treated with a silanizing reagent before testing to prevent methoprene attachment to the glassware. the no name key strain was compared with a susceptible laboratory strain of oc. taeniorhynchus. five serial dilutions (0.0100, 0.0050, 0.0010, 0.0005, and 0.0001 microg/ml) and an untreated control were evaluated. tests ...200212083352
evaluation of various models of propane-powered mosquito traps.large cage and field studies were conducted to determine the efficacy of various models of propane-powered mosquito traps. these traps utilized counterflow technology in conjunction with catalytic combustion to produce attractants (carbon dioxide, water vapor, and heat) and a thermoelectric generator that converted excess heat into electricity for stand-alone operation. the cage studies showed that large numbers of aedes aegypti and ochlerotatus taeniorhynchus were captured and that each progres ...200212125861
mosquito vector control and biology in latin america--a twelfth symposium. denver, colorado, february 2002. summaries.the 12th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 68th annual meeting in denver, co, in february 2002. the principal objective, as for the previous 11 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 35 presentations that were given orally in spanish or presented as posters by participants ...200212322934
effectiveness and residual activity comparison of granular formulations of insect growth regulators pyriproxyfen and s-methoprene against florida mosquitoes in laboratory and outdoor conditions.effectiveness and residual activity tests of granular formulations of 2 insect growth regulators (igrs), s-methoprene and pyriproxyfen, against laboratory-reared larvae of 5 colonized mosquitoes, aedes aegypti, aedes albopictus, aedes taeniorhynchus, anopheles quadrimaculatus, and culex nigripalpus, were conducted in the laboratory and outdoors in plastic tubs. culex quinquefasciatus was exposed to these two igrs in the laboratory only. each igr formulation was applied at 0.02 and 0.05 ppm activ ...200212322941
a mark-release-recapture experiment with anopheles sinensis in the northern part of gyeonggi-do, korea.in order to study the range of flight and feeding activity of anopheles sinensis, the dispersal experiment was conducted in paju city, located in the northern part of gyeonggi-do, republic of korea, during the period of 7th to 28th september 1998. unfed females an. sinensis were collected in cowshed and released after being marked with fluorescent dye at 23:00 hours on the same day. released female mosquitoes were recaptured everyday during 21 days using light traps, which were set at 10 sites i ...012325443
repellency of ir3535, kbr3023, para-menthane-3,8-diol, and deet to black salt marsh mosquitoes (diptera: culicidae) in the everglades national park.ir3535, kbr3023, para-menthane-3,8-diol (pmd), and deet were evaluated in controlled studies with human subjects (n = 5) for repellency to black salt marsh mosquitoes (ochlerotatus taeniorhynchus wiedemann), in the everglades national park, fl. in tests of 6-h duration, with an average mosquito biting pressure on exposed forearm skin of 19.5 (+/- 13.7) bites per minute, the mean percent repellencies (se) for ir3535, kbr3023, pdm, and deet was 88.6 (3.2), 97.5 (1.7), 89.2 (2.9), and 94.8 (2.5), r ...200212495189
glycosaminoglycan binding properties of natural venezuelan equine encephalitis virus isolates.equine-virulent, epidemic/epizootic strains of venezuelan equine encephalitis (vee) virus (veev) arise via mutation of progenitor enzootic strains that replicate poorly in equines. sequencing studies have implicated positively charged amino acids on the surface of the e2 envelope glycoprotein in the acquisition of equine virulence and viremia potential, suggesting that changes in binding to cell surface glycosaminoglycans (gags) may mediate vee emergence. therefore, we evaluated the binding of n ...200312502837
isolation of eee virus from ochlerotatus taeniorhynchus and culiseta melanura in coastal south carolina.a 1-year arbovirus study was conducted at the wedge plantation located in coastal south carolina to determine the occurrence and level of arbovirus activity in mosquito species inhabiting the site. mosquito species composition and temporal abundance were also determined. a total of 45,051 mosquitoes representing 27 species in 9 genera was collected and identified during 130 trap-nights between august, 1997, and july, 1998. the most abundant species was culex salinarius (n = 20,954) followed by o ...200312674532
vector competence of mexican and honduran mosquitoes (diptera: culicidae) for enzootic (ie) and epizootic (ic) strains of venezuelan equine encephalomyelitis virus.experimental studies evaluated the vector competence of ochlerotatus taeniorhynchus (wiedemann), culex cancer theobald, culex pseudes (dyar and knab), culex taeniopus dyar and knab, and a culex (culex) species, probably culex quinquefasciatus say, and culex nigripalpus theobald from chiapas, mexico, and tocoa, honduras, for epizootic (ic) and enzootic (ie) strains of venezuelan equine encephalomyelitis (vee) virus. culex pseudes was highly susceptible to infection with both the ic and ie strains ...200312943109
mosquitoes infected with west nile virus in the florida keys, monroe county, florida, usa.more than 30,000 mosquitoes in 22 species or species groups were collected from the florida keys, monroe county, fl, usa, in dry ice-baited light and gravid traps. dry ice-baited traps collected more mosquitoes than did gravid traps. west nile virus was detected in pools of anopheles atropos dyar & knab, deinocerites cancer theobald, and ochlerotatus taeniorhynchus (wiedemann).200312943117
studies on the dietary and endocrine regulation of autogenous reproduction in aedes taeniorhynchus (wied.). 196414188823
infection of malaria (anopheles gambiae s.s.) and filariasis (culex quinquefasciatus) vectors with the entomopathogenic fungus metarhizium anisopliae.current intra-domiciliary vector control depends on the application of residual insecticides and/or repellents. although biological control agents have been developed against aquatic mosquito stages, none are available for adults. following successful use of an entomopathogenic fungus against tsetse flies (diptera: glossinidae) we investigated the potency of this fungus as a biological control agent for adult malaria and filariasis vector mosquitoes.200314565851
venezuelan equine encephalitis.venezuelan equine encephalitis virus (veev) remains a naturally emerging disease threat as well as a highly developed biological weapon. recently, progress has been made in understanding the complex ecological and viral genetic mechanisms that coincide in time and space to generate outbreaks. enzootic, equine avirulent, serotype id veev strains appear to alter their serotype to iab or ic, and their vertebrate and mosquito host range, to mediate repeated vee emergence via mutations in the e2 enve ...200414651460
factors affecting immatures of ochlerotatus taeniorhynchus (diptera: culicidae) in san diego county, california.the effects of tide, temperature, rainfall, and salinity on the occurrence and abundance of immatures (instars and pupae) of the black salt marsh mosquito, ochlerotatus taeniorhynchus (wiedemann), were examined at a tidal pond in the tijuana estuary during 1996-1998. immatures were found between may and early september. tide had the greatest influence on immatures of this mosquito. eggs eclosed when tides averaged 1.96 +/- 0.10 (sd) m (1.83-2.19) 1-3 d before a flood tide. eclosion averaged once ...200314680101
vector competence of rural and urban strains of aedes (stegomyia) albopictus (diptera: culicidae) from são paulo state, brazil for ic, id, and if subtypes of venezuelan equine encephalitis virus.aedes albopictus (skuse) is an asiatic mosquito species that has spread and colonized all continents except antarctica. it has major public health importance because it is a potential vector of several pathogens. the objectives of our study were to analyze the vector competence of urban and rural strains of ae. albopictus from são paulo state (brazil) for venezuelan equine encephalitis virus (vee) subtypes ic, id, and if, and to evaluate the effect of infection with subtype ic of vee on mosquito ...200314680121
biochemical and cytoimmunological evidence for the control of aedes aegypti larval trypsin with aea-tmof.trypsin and chymotrypsin-like enzymes were detected in the gut of aedes aegypti in the four larval instar and pupal developmental stages. although overall the amount of trypsin synthesized in the larval gut was 2-fold higher than chymotrypsin, both enzymes are important in food digestion. feeding aea-trypsin modulating oostatic factor (tmof) to ae. aegypti and culex quinquefasciatus larvae inhibited trypsin biosynthesis in the larval gut, stunted larval growth and development, and caused mortali ...200414981657
a comparison of two spray nozzle systems used to aerially apply the ultra-low-volume adulticide fenthion.field experiments with the mosquito adulticide fenthion (baytex) compared the conventional flat-fan nozzle system (tee jet 8002ss) and a new high-pressure hollow-cone nozzle system (1/8 miss). ground deposition and aerial flux of the mosquito adulticide fenthion were measured up to 4.83 km downwind by using filter paper and yarn collectors, respectively. biological efficacy was investigated by using caged salt-marsh mosquitoes (ochlerotatus taeniorhynchus), and caged fiddler crabs (uca pugilator ...200415088702
a comparison of two ultra-low-volume spray nozzle systems by using a multiple swath scenario for the aerial application of fenthion against adult mosquitoes.two hydraulic spray nozzle systems, a flat fan and a high-pressure hollow cone, were used for ultra-low-volume application of the mosquito adulticide fenthion under a multiple swath scheme. eight swaths at 322-m intervals were applied from a height of 91 m to simulate operational conditions. deposition, effects on nontarget organisms (fiddler crabs), aerial flux, and mosquito (ochlerotatus taeniorhynchus) mortality were monitored for 8,230 m downwind, including the area under all 8 swaths. the f ...200415088703
genetic determinants of venezuelan equine encephalitis emergence.following a period of inactivity from 1973-1991, venezuelan equine encephalitis (vee) reemerged during the past decade in south america and mexico. experimental studies of vee virus (veev) infection of horses with virus strains isolated during these outbreaks have revealed considerable variation in the ability of equine-virulent, epizootic strains to exploit horses as efficient amplification hosts. subtype ic strains from recent outbreaks in venezuela and colombia amplify efficiently in equines, ...200415119762
differences in the effects of salinity on larval growth and developmental programs of a freshwater and a euryhaline mosquito species (insecta: diptera, culicidae).the effects of salinity on growth and development of the euryhaline ochlerotatus taeniorhynchus and the freshwater aedes aegypti are compared. o. taeniorhynchus grow larger, and have greater intrinsic growth rates, than a. aegypti. females of each species attain greater mass, take longer to develop, and have greater growth rates than do males. in o. taeniorhynchus, pupal mass, larval stage duration and growth rates (dry mass) increase with salinity, whereas growth rates (wet mass) remain constan ...200415159433
ph tolerances and regulatory abilities of freshwater and euryhaline aedine mosquito larvae.the ph regulatory abilities of two members of the mosquito tribe aedini, known to have dramatically different saline tolerances, are investigated. the freshwater mosquito aedes aegypti and the euryhaline ochlerotatus taeniorhynchus tolerate very similar ph ranges. both species complete larval development in waters ranging from ph 4 to ph 11, but naïve larvae always die in water of ph 3 or 12. across the ph range 4-11, the hemolymph ph of o. taeniorhynchus is maintained constant while that of a. ...200415159434
alkalization of larval mosquito midgut and the role of carbonic anhydrase in different species of mosquitoes.we have previously demonstrated the involvement of carbonic anhydrase (ca) in the alkalization mechanism of the aedes aegypti larval midgut. in this study, we used hansson's histochemistry to examine the distribution of the enzyme in the midgut of six different species of mosquito larvae (aedes aegypti, aedes albopictus, culex quinquefasciatus, culex nigripalpus, ochlerotatus taeniorhynchus, anopheles quadrimaculatus). additionally, we quantitated ca content in the gastric caeca, anterior and po ...200415171946
diagnostic dose of synergized d-phenothrin for insecticide susceptibility testing by bottle bioassay.the diagnostic dose of d-phenothrin synergized 1:1 with piperonyl butoxide for testing insecticide susceptibility of mosquitoes by bottle bioassay is reported for 2 mosquito species, culex quinquefasciatus and ochlerotatus taeniorhynchus. the diagnostic dose was defined as 2 times the 95% lethal concentration (lc95). lc50, lc90, and lc95 were estimated by probit analysis of dose response data. procedures for diluting the commercial-grade off-the-shelf pesticide in acetone, treating the bottles, ...200415264629
venezuelan equine encephalitis emergence: enhanced vector infection from a single amino acid substitution in the envelope glycoprotein.in 1993 and 1996, subtype ie venezuelan equine encephalitis (vee) virus caused epizootics in the mexican states of chiapas and oaxaca. previously, only subtype iab and ic vee virus strains had been associated with major outbreaks of equine and human disease. the iab and ic epizootics are believed to emerge via adaptation of enzootic (sylvatic, equine-avirulent) strains for high titer equine viremia that results in efficient infection of mosquito vectors. however, experimental equine infections w ...200415277679
experimental transmission of karshi and langat (tick-borne encephalitis virus complex) viruses by ornithodoros ticks (acari: argasidae).selected species of mosquitoes and ornithodoros ticks were evaluated for their potential to transmit karshi and langat (tick-borne encephalitis virus complex) viruses in the laboratory. although there was no evidence of replication of karshi virus in either of the two mosquito species tested [ochlerotatus taeniorhynchus (wiedemann) or culex pipiens (l.)], karshi virus replicated in and was transmitted by all three species of ornithodoros ticks tested (ornithodoros parkeri cooley, ornithodoros so ...200415535630
susceptibility of ochlerotatus taeniorhynchus (diptera: culicidae) to infection with epizootic (subtype ic) and enzootic (subtype id) venezuelan equine encephalitis viruses: evidence for epizootic strain adaptation.to test the hypothesis that adaptation to epizootic mosquito vectors mediates emergence of venezuelan equine encephalitis virus (veev) from enzootic progenitors, experimental infection studies were conducted to determine the susceptibility of ochlerotatus taeniorhynchus (wiedemann) to epizootic and enzootic strains. artificial blood meals containing epizootic subtype ic strains isolated during the 1962-1964, 1992-1993, and 1995 venezuelan/colombian epizootics and closely related venezuelan enzoo ...200415535633
efficient functional pseudotyping of oncoretroviral and lentiviral vectors by venezuelan equine encephalitis virus envelope proteins.murine oncoretroviruses and lentiviruses pseudotyped with envelope proteins of alphaviruses have shown great potential in providing broad-host-range, stable vectors for gene therapy. unlike vesicular stomatitis virus g protein-pseudotyped vectors, they are not neutralized by complement and do not appear to cause significant tissue damage. here we report the production of murine oncoretroviral and lentiviral vectors pseudotyped with the envelope proteins of venezuelan equine encephalitis virus (v ...200515613303
venezuelan equine encephalitis virus, southern mexico.equine epizootics of venezuelan equine encephalitis (vee) occurred in the southern mexican states of chiapas in 1993 and oaxaca in 1996. to assess the impact of continuing circulation of vee virus (veev) on human and animal populations, serologic and viral isolation studies were conducted in 2000 to 2001 in chiapas state. human serosurveys and risk analyses indicated that long-term endemic transmission of veev occurred among villages with seroprevalence levels of 18% to 75% and that medical pers ...200415663847
efficacy studies of vectobac 12as and teknar hp-d larvicides against 3rd-instar ochlerotatus taeniorhynchus and culex quinquefasciatus in small plot field studies.efficacy studies were conducted with vectobac 12as and teknar hp-d larvicides against 3rd-instar ochlerotatus taeniorhynchus and culex quinquefasciatus in small field test plots. the products were obtained off the shelf from distributors and had different lot numbers. they were evaluated over a 2-year period in spring 2002 and 2003. application rates were 0.29, 0.58, and 1.10 liter/ha and evaluations were made 24 and 48 h after treatment. both products performed well in these studies, with vecto ...200415669386
epidemiology of west nile virus in connecticut: a five-year analysis of mosquito data 1999-2003.two hundred and ten isolations of west nile virus (wnv) were obtained from 17 mosquito species in six genera in statewide surveillance conducted in connecticut from june through october, 1999-2003. culex pipiens (86), culex salinarius (32), culex restuans (26), culiseta melanura (32), and aedes vexans (12) were implicated as the most likely vectors of wnv in the region based on virus isolation data. culex pipiens was abundant from july through september and is likely involved in early season enz ...200415682518
passive prophylaxis with permethrin-treated tents reduces mosquito bites among north american summer campers.over 2 million adolescents participate in summer-camp experiences, placing themselves at risk for mosquito-borne illness. insect repellent is recommended but often not used. it is unknown whether permethrin treatment of a location, such as a campsite, provides passive prophylaxis reducing mosquito bites among all persons in the immediate vicinity.015813141
colonization of ochlerotatus taeniorhynchus from riohacha, colombia.the main objective of the present work was to establish and maintain a colony of ochlerotatus taeniorhynchus, riohacha strain, under laboratory conditions and to study its life cycle. the colony's insectary was established from adult male and female mosquitoes collected from peripheral neighborhoods of riohacha, colombia. environmental conditions established in the insectary were 26.5 degrees c average temperature, 80% average relative humidity, and 12 h photoperiodicity. eight continuous genera ...200515825758
entomopathogenic fungi for mosquito control: a review.fungal diseases in insects are common and widespread and can decimate their populations in spectacular epizootics. virtually all insect orders are susceptible to fungal diseases, including dipterans. fungal pathogens such as lagenidium, coelomomyces and culicinomyces are known to affect mosquito populations, and have been studied extensively. there are, however, many other fungi that infect and kill mosquitoes at the larval and/or adult stage. the discovery, in 1977, of the selective mosquito-pa ...200415861235
susceptibility of ochlerotatus taeniorhynchus and culex nigripalpus for eeverglades virus.everglades virus (evev), an alphavirus in the venezuelan equine encephalitis (vee) complex, is a mosquito-borne human pathogen endemic to south florida. field isolations of evev from culex (melanoconion) cedecei and laboratory susceptibility experiments established this species as its primary vector. however, isolates of evev from ochlerotatus taeniorhynchus and culex nigripalpus, more abundant and widespread species in south florida, suggested that they also transmit evev and could infect many ...200516014824
evaluation of methods to assess transmission potential of venezuelan equine encephalitis virus by mosquitoes and estimation of mosquito saliva titers.determining the dose of an arbovirus transmitted by a mosquito is important to design transmission and pathogenesis studies simulating natural infection. several different artificial infection and transmission methods used to assess vector competence and to estimate the dose injected during mosquito feeding have not been fully evaluated to determine whether they accurately reflect natural transmission. additionally, it is not known whether different mosquito vectors transmit similar amounts of a ...200516014828
morphological and molecular characterization of a cypovirus (reoviridae) from the mosquito uranotaenia sapphirina (diptera: culicidae).a novel cypovirus has been isolated from the mosquito uranotaenia sapphirina (uscpv) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. the production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. the virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a c ...200516014906
west nile virus surveillance, guadeloupe, 2003-2004.we conducted extensive surveillance for west nile virus infection in equines and chickens in guadeloupe in 2003-2004. we showed a high seroprevalence in equines in 2003 related to biome, followed by a major decrease in virus circulation in 2004. no human or equine cases were reported during the study.200516022789
carbonic anhydrase in the adult mosquito midgut.we have previously demonstrated the involvement of carbonic anhydrase (ca) in larval mosquito midgut physiology. in this study, we used hansson's histochemistry to examine the distribution of the enzyme in the midgut of aedes aegypti, aedes albopictus, culex quinquefasciatus, culex nigripalpus, ochlerotatus taeniorhynchus, anopheles albimanus and anopheles quadrimaculatus adult mosquitoes. additionally, we quantitated ca content in the anterior and posterior midgut of adult males and females fro ...200516109888
epidemiology of heartworm: what is happening in south america and mexico?canine heartworm has been known to exist in the americas for more than 150 years (1847), and the first south american report was published approximately 30 years later (1875). the first human case was reported in 1887 and the first feline case in 1921. coincidently, these two reports were from brazil, where most surveys of the disease during the past two decades have been conducted in dogs. feline cases are seldom found in the south american literature, although feline heartworm disease has been ...200516198820
west nile virus-infected mosquitoes, louisiana, 2002.human cases of west nile virus (wnv) disease appeared in st. tammany and tangipahoa parishes in southeastern louisiana in june 2002. cases peaked during july, then rapidly declined. we conducted mosquito collections from august 3 to august 15 at residences of patients with confirmed and suspected wnv disease to estimate species composition, relative abundance, and wnv infection rates. a total of 31,215 mosquitoes representing 25 species were collected by using primarily gravid traps and co2-bait ...200516229769
susceptibility of psorophora confinnis (diptera: culicidae) to infection with epizootic (subtype ic) and enzootic (subtype id) venezuelan equine encephalitis viruses.to test the hypothesis that adaptation to epizootic mosquito vectors mediates the emergence of venezuelan equine encephalitis virus (family togaviridae, genus alphavirus, veev) from enzootic progenitors, the susceptibility of the epizootic vector psorophora confinnis (lynch-arribalzaga) to epizootic versus enzootic strains was evaluated. artificial bloodmeals containing subtype ic strains isolated during the 1962-1964, 1992-1993, and 1995 venezuelan/colombian epizootics and closely related venez ...200516365999
identification of aedes aegypti and its respective life stages by real-time polymerase chain reaction.an aedes aegypti-specific, fluorogenic probe hydrolysis (taq-man), polymerase chain reaction assay was developed for real-time screening using a field-deployable thermocycler. laboratory-based testing of a. aegypti, a. aegypti (trinidad strain), culex pipiens, culex quinquefasciatus, anopheles stephensi, and ochlerotatus taeniorhynchus individual adult mosquitoes and mixed pools (n = 10) demonstrated 100% concordance in both in vitro sensitivity (six of six samples) and specificity (10 of 10 sam ...200516491948
the evolution of viral emergence. 200616567658
evaluation of habitat management strategies for the reduction of malaria vectors in northern belize.mowing and burning of emergent vegetation were evaluated as potential management strategies for the control of the malaria vector, anopheles vestitipennis, in northern belize, central america. the primary aim was reduction of tall dense macrophytes (dominated by typha domingensis) as preferred larval habitat for an. vestitipennis. nine experimental plots were established in a typha marsh in orange walk district, belize. three plots were burned, three were treated by subaquatic mowing, and three ...200516599158
relative abundance of mosquito species (diptera: culicidae) on big pine key, florida, u.s.a.species diversity of mosquito (diptera: culicidae) collections in carbon dioxide-baited light traps was investigated on big pine key, florida, from 2000 - 2004. twenty species of mosquitoes were collected during this study, the most commonly collected being anopheles atropos, culex bahamensis, deinocerites cancer, and ochlerotatus taeniorhynchus. ochlerotatus taeniorhynchus is the dominant species at this location. for most of the mosquito species, more individuals were collected during the "hig ...200516599171
targeted trapping of mosquito vectors in the chesapeake bay area of maryland.most adult mosquito surveillance in maryland is performed using dry ice-baited or unbaited centers for disease control (cdc) miniature light traps suspended approximately 1.5 m above the ground. however, standardized trapping methods may miss mosquito species involved in disease transmission cycles. during a 2-yr study, the effectiveness of the olfactory attractant 1-octen-3-ol alone and in combination with carbon dioxide was evaluated for collecting mosquito vector species. in addition, trap he ...200616619593
efficacy studies of aquaprene (emulsifiable concentrate and wettable powder) insect growth regulator formulations against third- and fourth-stage larvae of ochlerotatus taeniorhynchus in small-plot field studies.efficacy studies were conducted with aquaprene emulsifiable concentrate (ec) (33.6% active ingredient [ai]) and wettable powder (wp) (40% ai) (s)-methoprene insect growth regulator formulations against larval ochlerotatus taeniorhynchus in small field test plots. aquaprene ec was applied at 7.18 and 9.57 g/acre. approximately one thousand 4th-stage larvae were added to each plot before treatment, and 24 h later pupae were collected to determine emergence inhibition. at both applications rates, a ...200616646333
secretion of water and ions by malpighian tubules of larval mosquitoes: effects of diuretic factors, second messengers, and salinity.the effects of changes in the salinity of the rearing medium on malpighian tubule fluid secretion and ion transport were examined in larvae of the freshwater mosquito aedes aegypti and the saltwater species ochlerotatus taeniorhynchus. for unstimulated tubules of both species, the k(+) concentration of secreted fluid was significantly lower when larvae were reared in 30% or 100% seawater (o. taeniorhynchus only), relative to tubules from freshwater-reared larvae. the na(+) concentration of secre ...201316691529
influence of fluctuating salinity on insecticide tolerance of two euryhaline arthropods.among many other abiotic variations in an estuarine ecosystem, osmotic stress is an inescapable part of life. organisms living in such environments must cope with changing osmotic conditions by either behavioral or physiological adaptations. pollutants may increase the physiological stresses that an osmoregulating animal may encounter. we have developed a flow-through system that exposes test species to insecticides and continuously changing salinity conditions. this system has provided an insig ...200616813307
genome of invertebrate iridescent virus type 3 (mosquito iridescent virus).iridoviruses (ivs) are classified into five genera: iridovirus and chloriridovirus, whose members infect invertebrates, and ranavirus, lymphocystivirus, and megalocytivirus, whose members infect vertebrates. until now, chloriridovirus was the only iv genus for which a representative and complete genomic sequence was not available. here, we report the genome sequence and comparative analysis of a field isolate of invertebrate iridescent virus type 3 (iiv-3), also known as mosquito iridescent viru ...200616912294
venezuelan equine encephalitis virus transmission and effect on pathogenesis.quantifying the dose of an arbovirus transmitted by mosquitoes is essential for designing pathogenesis studies simulating natural infection of vertebrates. titration of saliva collected in vitro from infected mosquitoes may not accurately estimate titers transmitted during blood feeding, and infection by needle injection may affect vertebrate pathogenesis. we compared the amount of venezuelan equine encephalitis virus collected from the saliva of aedes taeniorhynchus to the amount injected into ...200616965696
the effect of pesticide residue on caged mosquito bioassays.wind tunnel experiments showed that secondary pickup of insecticide residue by mosquitoes in cage bioassays had a significant effect on mortality. cage bioassays using adult ochlerotatus taeniorhynchus (wiedemann) investigated the effect of exposure time to a contaminated surface. cages were dosed in a wind tunnel using the lc50 for naled (0.124 mg a.i./ml) and an lc25 (0.0772 mg a.i./ml) for naled. half of the bioassay mosquitoes were moved directly into clean cages with the other half remainin ...200617067048
traps and trapping techniques for adult mosquito control.an overview is presented of the recent advancements in research activities conducted to evaluate mosquito traps, insecticide-impregnated targets baited with combinations of attractants, and strategies for using mass trapping techniques for adult mosquito population management. technologies that use semiochemicals (attractants), traps and targets, and mass trapping are relatively new for management of adult mosquito populations. to date, emphasis has been placed primarily on developing barriers o ...200617067051
application of boric acid baits to plant foliage for adult mosquito control.boric acid (1%) in 5% sugar water bait solution was applied as a spray to the foliage, stems, and other surfaces of plants for control of adult aedes albopictus, culex nigripalpus, and ochlerotatus taeniorhynchus. initial studies outdoors in small (1.42-m3) screened cages showed that exposure of male and female mosquitoes to 1% boric acid bait for 48 h resulted in 80 to 100% mortality in ae. albopictus and > or = 98% mortality in cx. nigripalpus and oc. taeniorhynchus. at 48 h posttreatment, in ...200617067052
drosophila melanogaster methoprene-tolerant (met) gene homologs from three mosquito species: members of pas transcriptional factor family.the methoprene-tolerant (met) gene in drosophila melanogaster has been shown to function in juvenile hormone (jh) action. met homologs were isolated from three mosquito species, culex pipiens, aedes aegypti and anopheles gambiae. sequence similarity was found to be high in bhlh and pas conserved domains, and the majority of the 7-9 introns in aamet and agmet are located in either identical or similar positions, indicating evolutionary relatedness. sequence comparison with met and the similar ger ...200717166512
experimental cutaneous bacillus anthracis infections in hairless hrs/j mice.previous studies of experimental bacillus anthracis cutaneous infections in mice have implicated hair follicles as a likely entry site. hairless hrs/j mice were used to investigate this possibility because of their non-functional hair follicles that lack penetrating hair shafts. these mice also have diminished macrophage function, increased susceptibility to listeria, and enhanced neutrophil responses. hrs/j and balb/c mice were found to be resistant to epicutaneous inoculation with bacillus ant ...200717244341
dengue virus type 2: replication and tropisms in orally infected aedes aegypti mosquitoes.to be transmitted by its mosquito vector, dengue virus (denv) must infect midgut epithelial cells, replicate and disseminate into the hemocoel, and finally infect the salivary glands, which is essential for transmission. the extrinsic incubation period (eip) is very relevant epidemiologically and is the time required from the ingestion of virus until it can be transmitted to the next vertebrate host. the eip is conditioned by the kinetics and tropisms of virus replication in its vector. here we ...200717263893
distinct roles of broad isoforms in regulation of the 20-hydroxyecdysone effector gene, vitellogenin, in the mosquito aedes aegypti.we investigated the role of the mosquito broad (br) gene in regulating the 20-hydroxyecdysone (20e) effector vitellogenin (vg) gene. injection of double-stranded rna corresponding to the br isoform z2 led to a significant decrease in expression of the vg gene at 8 and 24h post-blood meal. knockdown of z1 or z4 resulted in enhanced vg expression beyond its normal expression time. in vitro studies suggested that the effects of br require its direct binding to the vg promoter, as well as protein-pr ...200717303321
interisland dispersal of the black salt marsh mosquito, ochlerotatus taeniorhynchus (diptera: culicidae) in the florida keys.mark-release-recapture experiments were conducted in 2001 and 2002 to determine whether ochlerotatus taeniorhynchus, black salt marsh mosquitoes, were dispersing from uninhabited islands in the key deer national wildlife refuge to inhabited islands within monroe county, florida. an estimated 1,658,000 mosquitoes were marked during 2001, and an estimated 300,000 mosquitoes were marked during 2002. recapture rates were 0.0061% and 0.0117%, respectively. oc. taeniorhynchus disperse from uninhabited ...200617304926
serologic evidence of widespread everglades virus activity in dogs, florida.everglades virus (evev), an alphavirus in the venezuelan equine encephalitis complex, circulates among rodents and vector mosquitoes in florida and occasionally infects humans. it causes febrile disease, sometimes accompanied by neurologic manifestations. although previous surveys showed high seroprevalence in humans, evev infections may be underdiagnosed because the disease is not severe enough to warrant a clinic visit or the undifferentiated presentations complicate diagnosis. documented evev ...200617326938
field tests of malathion and permethrin applied via a truck-mounted cold fogger to both open and vegetated habitats.field tests of formulated permethrin (permanone 30:30) and malathion applied by ground ultra-low volume equipment were conducted using caged, laboratory-reared, adult ochlerotatus taeniorhynchus. cages were placed in an open field and also in semi-dense scrub pine. two doses, a high and a medium dose of each compound, were applied as suggested by the label. regardless of application rate, neither chemical provided satisfactory control under the canopy. the average mortality count at the open sit ...200717536368
infection and dissemination of venezuelan equine encephalitis virus in the epidemic mosquito vector, aedes taeniorhynchus.the mosquito aedes taeniorhynchus is an important epidemic vector of venezuelan equine encephalitis virus (veev), but detailed studies of its infection are lacking. we compared infection by an epidemic veev strain to that by an enzootic strain using virus titrations, immunohistochemistry, and a virus expressing the green fluorescent protein. ae. taeniorhynchus was more susceptible to the epidemic strain, which initially infected the posterior midgut and occasionally the anterior midgut and cardi ...200717620651
reduced infection in mosquitoes exposed to blood meals containing previously frozen flaviviruses.the increased difficulty and expense of using live animals for delivering infectious blood meals in arthropod-borne virus vector competence experiments has resulted in an increase in the use of artificial feeding systems. compared to live hosts, artificial systems require higher viral titers to attain mosquito infection, thereby limiting the utility of such systems with low or moderate titer virus stocks. based on the report that freshly propagated virus is more infectious than previously frozen ...200717686541
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