Publications

endophytic colonization of barley (hordeum vulgare) roots by the nematophagous fungus pochonia chlamydosporia reveals plant growth promotion and a general defense and stress transcriptomic response.	plant crop yields are negatively conditioned by a large set of biotic and abiotic factors. an alternative to mitigate these adverse effects is the use of fungal biological control agents and endophytes. the egg-parasitic fungus pochonia chlamydosporia has been traditionally studied because of its potential as a biological control agent of plant-parasitic nematodes. this fungus can also act as an endophyte in monocot and dicot plants, and has been shown to promote plant growth in different agrono ...	2015	25982739
fungi predatory activity on embryonated toxocara canis eggs inoculated in domestic chickens (gallus gallus domesticus) and destruction of second stage larvae.	the objective of this study was to evaluate the infectivity of toxocara canis eggs after interacting with isolated nematophagous fungi of the species duddingtonia flagrans (ac001) and pochonia chlamydosporia (vc4), and test the predatory activity of the isolated ac001 on t. canis second stage larvae after 7 days of interaction. in assay a, 5000 embryonated t. canis eggs previously in contact with the ac001 and vc4 isolated for 10 days were inoculated into domestic chickens (gallus gallus domesti ...	2015	26032943
yellow pigment aurovertins mediate interactions between the pathogenic fungus pochonia chlamydosporia and its nematode host.	nematophagous fungi are globally distributed soil fungi and well-known natural predators of soil-dwelling nematodes. pochonia chlamydosporia can be found in diverse nematode-suppressive soils as a parasite of nematode eggs and is one of the most studied potential biological control agents of nematodes. however, little is known about the functions of small molecules in the process of infection of nematodes by this parasitic fungus or about small-molecule-mediated interactions between the pathogen ...	2015	26151481
[effects of pesticides and plant bio-stimulants on the germination of chlamydospores and in vitro development of the nematophagous fungus pochonia chlamydosporia].	the effects of pesticides and plant bio-stimulants used in protected vegetable production systems on the fungus pochonia chlamydosporia are unknown.	2015	26422324
whole genome annotation and comparative genomic analyses of bio-control fungus purpureocillium lilacinum.	the fungus purpureocillium lilacinum is widely known as a biological control agent against plant parasitic nematodes. this research article consists of genomic annotation of the first draft of whole genome sequence of p. lilacinum. the study aims to decipher the putative genetic components of the fungus involved in nematode pathogenesis by performing comparative genomic analysis with nine closely related fungal species in hypocreales.	2015	26607873
the complete mitochondrial genome of the nematophagous fungus lecanicillium saksenae.	lecanicillium saksenae cgmcc5329 is a useful biological control agent against plant-parasitic nematodes. the complete mitogenome sequence of l. saksenae is reported for the first time. the mitochondrial genome is 25 919 bp long with 14 typical protein-coding genes, an intronic orf coding for a putative ribosomal protein (rps3), 2 ribosomal rna genes and a set of 26 transfer rna genes. the phylogeny based on 12 protein-coding genes (except the loss of other two genes in acremonium implicatum) sug ...	2017	26709967
characterization of soil suppressiveness to root-knot nematodes in organic horticulture in plastic greenhouse.	the fluctuation of meloidogyne population density and the percentage of fungal egg parasitism were determined from july 2011 to july 2013 in two commercial organic vegetable production sites (m10.23 and m10.55) in plastic greenhouses, located in northeastern spain, in order to know the level of soil suppressiveness. fungal parasites were identified by molecular methods. in parallel, pot tests characterized the level of soil suppressiveness and the fungal species growing from the eggs. in additio ...	2016	26925080
chitosan enhances parasitism of meloidogyne javanica eggs by the nematophagous fungus pochonia chlamydosporia.	pochonia chlamydosporia (pc), a nematophagous fungus and root endophyte, uses appressoria and extracellular enzymes, principally proteases, to infect the eggs of plant parasitic nematodes (ppn). unlike other fungi, pc is resistant to chitosan, a deacetylated form of chitin, used in agriculture as a biopesticide to control plant pathogens. in the present work, we show that chitosan increases meloidogyne javanica egg parasitism by p. chlamydosporia. using antibodies specific to the pc enzymes vcp1 ...	2016	27020158
cell wall composition plays a key role on sensitivity of filamentous fungi to chitosan.	chitosan antifungal activity has been reported for both filamentous fungi and yeast. previous studies have shown fungal plasma membrane as main chitosan target. however, the role of the fungal cell wall (cw) in their response to chitosan is unknown. we show that cell wall regeneration in neurospora crassa (chitosan sensitive) protoplasts protects them from chitosan damage. caspofungin, a β-1,3-glucan synthase inhibitor, showed a synergistic antifungal effect with chitosan for n. crassa but not f ...	2016	27259000
arabidopsis thaliana root colonization by the nematophagous fungus pochonia chlamydosporia is modulated by jasmonate signaling and leads to accelerated flowering and improved yield.	pochonia chlamydosporia has been intensively studied in nematode control of different crops. we have investigated the interaction between p. chlamydosporia and the model system arabidopsis thaliana under laboratory conditions in the absence of nematodes. this study demonstrates that p. chlamydosporia colonizes a. thaliana. root colonization monitored with green fluorescent protein-tagged p. chlamydosporia and quantitative pcr (qpcr) quantitation methods revealed root cell invasion. fungal inocul ...	2017	27456071
cazyme content of pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism.	pochonia chlamydosporia is a soil fungus with a multitrophic lifestyle combining endophytic and saprophytic behaviors, in addition to a nematophagous activity directed against eggs of root-knot and other plant parasitic nematodes. the carbohydrate-active enzymes encoded by the genome of p. chlamydosporia suggest that the endophytic and saprophytic lifestyles make use of a plant cell wall polysaccharide degradation machinery that can target cellulose, xylan and, to a lesser extent, pectin. this e ...	2016	27668983