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pathogenicity of the entomopathogenic fungi paecilomyces spp. and beauveria bassiana against the silverleaf whitefly, bemisia argentifolii.pathogenicities of three species of entomopathogenic fungi against preimaginal bemisia argentifolii were measured and compared. third-instar nymphs on excised leaves of hibiscus rosa-sinensis were exposed to spray applications of 14 isolates of beauveria bassiana, 22 isolates of paecilomyces fumosoroseus, and five isolates of paecilomyces farinosus. b. bassiana and p. fumosoroseus isolates of diverse origins were highly pathogenic to the whitefly nymphs; median lethal doses of 14 of the 22 p. fu ...19989538026
characterization of monoclonal antibodies against cell wall epitopes of the insect pathogenic fungus, nomuraea rileyi: differential binding to fungal surfaces and cross-reactivity with host hemocytes and basement membrane components.monoclonal antibodies (mabs) were generated against epitopes on yeast-like hyphal bodies and hyphae of the entomopathogenic hyphomycete, nomuraea rileyi. two mabs (4c10, 2h4) bind to epitopes common to both hyphal bodies and hyphae, whereas mab 4e9 binds only to hyphal surfaces. 4c10 and 2h4 appear to be directed towards carbohydrate portions of cell surface mannoproteins, as evidenced by similarities in staining patterns between these mabs and concanavalin a on western blots of n. rileyi cell w ...19989548369
factors affecting conidial production of beauveria bassiana from fungus-killed cadavers of rhodnius prolixus.the epizootic potential of beauveria bassiana for control of the triatomine vectors of chagas' disease was investigated by studying the effects of both biotic and abiotic factors on the recycling of a highly virulent fungal isolate from fungus-killed cadavers of rhodnius prolixus. the conidial production of b. bassiana from mummified cadavers of r. prolixus required high rh levels of at least 96.5% rh. at 97% rh and 25 degrees c, the amount of conidia per insect ranged from 5.3 x 10(6) (on first ...19989709008
localization of auxotrophic and benomyl resistance markers through the parasexual cycle in the beauveria bassiana (bals.) vuill entomopathogen.genetic studies to localize auxotrophic markers and resistance to benomyl fungicide and to analyze gene transfer on the beauveria bassiana deuteromycete were carried out using the parasexual cycle. parasexual crosses among strains with complementary genetic markers resulted in vigorous heterocaryons. selection of the segregant products was made using two methodologies: total isolation and parental elimination. colonies with recombinant traits were recovered directly from the heterocaryon in all ...19989709011
pyridovericin and pyridomacrolidin: novel metabolites from entomopathogenic fungi, beauveria bassiana. 19989711226
characterization and structure of the mitochondrial small rrna gene of the entomopathogenic fungus beauveria bassiana.the entire mitochondrial (mt) small ribosomal rna (srrna) gene from the entomopathogenic fungus beauveria bassiana was sequenced. alignment of the sequence to those of other filamentous fungi revealed gross length differences in their respective products. construction of a secondary structural model showed that these differences were restricted to known variable srrna subdomains. several features were identified that were common only to the hyphomycetous fungi examined. phylogenetic analysis ind ...19989729784
differentiation of beauveria bassiana isolates from the darkling beetle, alphitobius diaperinus, using isozyme and rapd analysestwo natural genetic markers, isozymes and rapd, were utilized to differentiate 24 strains of beauveria bassiana (deuteromycotina: hyphomycetes) collected from the darkling beetle, alphitobius diaperinus (coleoptera: tenebrionidae), from poultry houses in north carolina and west virginia. nine enzyme systems were screened, of which alkaline phosphatase, alpha- and beta-esterase, and glucose phosphate isomerase gave well-resolved, scorable bands. a total of 26 isozyme bands was generated by these ...19989784340
conidial attachment of metarhizium anisopliae and beauveria bassiana to the larval cuticle of diaprepes abbreviatus (coleoptera: curculionidae) treated with imidacloprida series of experiments was conducted to determine the effect of imidacloprid on the number of metarhizium anisopliae and beauveria bassiana conidia found on the cuticle of first instar diaprepes abbreviatus following different treatments. larvae treated with m. anisopliae conidia and imidacloprid by dipping removed significantly fewer conidia from their cuticle when in contact with soil or a food source compared with fungal-treated larvae alone. in addition, more m. anisopliae and b. bassiana c ...19989784344
antimicrobial activity of exocrine glandular secretions, hemolymph, and larval regurgitate of the mustard leaf beetle phaedon cochleariaelarvae and adults of the mustard leaf beetle phaedon cochleariae (coleoptera: chrysomelidae) possess exocrine glands with secretions which are used in defense against predators. this study addressed the question whether these defensive secretions also display antimicrobial activity. additionally, the effects of larval and adult hemolymph and larval regurgitate toward microorganisms were examined. the larval glandular secretion showed growth-inhibitory activity against the gram-negative bacterium ...19989784354
beauveria bassiana (hyphomycetes) as a possible agent for biological control of chagas disease vectors.beauveria bassiana (balsamo) vuillemin (isolate cg306) was tested on 3rd instars of 9 triatoma spp., 4 rhodnius spp., 2 panstrongylus spp. and dipetalogaster maxima (uhler) at 25 degrees c and 50% rh. quantitative sporulation of the fungus on cadavers was studied at 25 degrees c and 97% rh. mortality, estimates of survival time, and conidial production on cadavers differed significantly among the genera and species tested. panstrongylus herreri wygodzinsky, dipetalogaster maxima, triatoma pictur ...19989835689
dermatophytes isolated from selected ruminants in india.a survey was carried out in different areas of uttar pradesh for isolating and identifying dermatophytes from ruminants. out of 102 skin scrapings, collected from various animals, 4/22 cattle, 1/9 buffalo, 14/44 sheep and 2/28 goats were koh positive. in respect of the cattle, the culture positive samples yielded one strain each of trichophyton mentagrophytes, t. rubrum, t. simii and two strains of microsporum gypseum. from the buffaloes examined, only one case yielded a dermatophyte and it was ...19989850594
field assessment of beauveria bassiana (balsamo) vuillemin and potential synergism with diflubenzuron for control of savanna grasshopper complex (orthoptera) in mali.in large-scale field trials in mali, formulated conidia of beauveria bassiana (balsamo) vuillemin strain gha were tested against unconfined grasshopper populations in field plots of 10 ha each. the trials compared b. bassiana conidia formulated in a mineral oil carrier, formulated diflubenzuron, a combination of b. bassiana plus formulated diflubenzuron, and fenitrothion. a total of 30 different species of grasshoppers occurred in the experimental plots, of which 3 were dominant in all plots, na ...19999878287
the structures of pyridovericin and pyridomacrolidin, new metabolites from the entomopathogenic fungus, beauveria bassiana. 19989918400
selection of beauveria bassiana and metarhizium anisopliae isolates to control triatoma infestans.twenty three isolates of beauveria bassiana and 13 isolates of metarhizium anisopliae were tested on third instar nymphs of triatoma infestans, a serious vector of chagas disease. pathogenicity tests at saturated humidity showed that this insect is very susceptible to fungal infection. at lower relative humidity (50%), conditions expected in the vector microhabitat, virulence was significantly different among isolates. cumulative mortality 15 days after treatment varied from 17.5 to 97.5%, and e ...19989921313
sporulation of beauveria bassiana on cadavers of triatoma infestans after infection at different temperatures and doses of inoculum. 199910066403
genetic variation in beauveria bassiana populations associated with the darkling beetle, alphitobius diaperinus.a study was conducted to assess genetic variation within and among populations of beauveria bassiana (deuteromycotina: hyphomycetes) associated with the darkling beetle, alphitobius diaperinus (coleoptera: tenebrionidae), using rapd markers. a hierarchical collection of samples (strains from the same insect specimen, from insects from the same location, and from insects from different locations) was obtained from infected beetles from north carolina (nc) and west virginia (wv), usa. ten primers ...199910222180
insects and other arthropods used as drugs in korean traditional medicine.insects and other arthropods appear in pharmacopoeias of korean traditional medicine, but little was known about their use in modern south korea. interviews were conducted with 20 traditional medicine doctors at clinics in south korea's kyeong dong shijang in seoul--one of the world's largest traditional drug markets--to learn about current patterns of usage. seventeen products are prescribed and the use of arthropod drugs is stable or increasing. centipedes (scolopendra spp.) used primarily to ...199910404418
lectin from beauveria bassiana mycelium recognizes thomsen-friedenreich antigen and related structures.a lectin was isolated from the mycelium of the stationary growing enthomopathogenic fungus beauveria bassiana by extraction, chromatography on qae-sephadex a-25, salt precipitation, and hydrophobic chromatography on phenyl-sepharose 4b. the beauveria bassiana lectin (bbl) is a 15 kda glycoprotein rich in hydrophobic amino acids, without detectable amount of methionine. it contains 12.6% of carbohydrates including galactose and mannose. isoelectric point was found at ph 7.1. the lectin is stable ...199910425710
the concept of docking/protecting groups in biohydroxylation.a general principle for biohydroxylation, in which time-consuming screening and enrichment techniques are avoided, is demonstrated by the introduction of a docking/protecting group into the substrate. this facilitates acceptance by the microorganism and allows the use of a narrow range of microorganisms, for example beauveria bassiana attc 7159 (b. b.), for the hydroxylation of compounds with diverse structures. after the biohydroxylation, the docking/protecting group is removed (see scheme).199910508375
water and temperature relations of growth of the entomogenous fungi beauveria bassiana, metarhizium anisopliae, and paecilomyces farinosusthe effects of temperature (5-50 degrees c), water availability (0. 998-0.88 water activity, a(w)), and a(w) x temperature interactions (15-45 degrees c) on growth of three entomogenous fungi, beauveria bassiana, metarhizium anisopliae, and paecilomyces farinosus, were evaluated on a sabouraud dextrose-based medium modified with the ionic solute kcl, the non-ionic solute glycerol, and an inert solute, polyethylene glycol (peg) 600. the temperature ranges for growth of b. bassiana, m. anisopliae, ...199910534413
drosophila cecropin as an antifungal agent.the effects of drosophila and hyalophora cecropins were tested against different fungi, both insect pathogens and fungi from the normal environment of drosophila. the fungi were generally found to be as susceptible to the cecropins as most bacteria, the only exception being the insect pathogen beauveria bassiana which is completely resistant. this is also the only fungus tested which is virulent to drosophila, giving 100% lethality within 5 days after injection. lethal concentrations of cecropin ...199910560137
constitutive and heat-inducible heat shock element binding activities of heat shock factor in a group of filamentous fungi.this study represents the initial characterization of the heat shock factor (hsf) in filamentous fungi. we demonstrate that hsfs from beauveria bassiana, metarhizium anisopliae, tolypocladium nivea, paecilomyces farinosus, and verticillium lecanii bind to the heat shock element (hse) constitutively (non-shocked), and that heat shock resulted in increased quantities and decreased mobility of hsf-hse complexes. the monomeric molecular mass of both heat-induced and constitutive hsfs was determined ...199910590835
[the effect of infestation by mixed culture of beauveria bassiana and paecilomyces farinosus on reduction in numbers of experimental culture blattella germanica l].in this paper results of infestation of blatella germanica l with mixed culture of insecticidal fungi belonging to one two species are presented. in the first stage of experiment the insects were infested first with b. bassiana spores and later with p. farinosus spores (strains l and p) was used as first and b. bassiana as second with time sequence of only 24 and 72 hours. out of one species cultures tests were done alternately with strains l and p of p. farinosus. the control insects were alway ...199910628229
clues for the existence of two different epoxide hydrolase activities in the fungus beauveria bassiana.epoxide hydrolase activity was produced during the exponential and stationary growth phases of the fungus beauveria bassiana atcc 7159. it was completely cell-associated. after cell disruption epoxide hydrolase activity was recovered in both the cell debris (eh "a") and the soluble fraction (eh "b"), but not in the membrane fraction. activity assays of these fractions with two different substrates indicated that their substrate specificity, as well as the corresponding e value and, to a lesser e ...200010713216
dependence of the entomopathogenic fungus, beauveria bassiana, on high humidity for infection of rhodnius prolixus.the impact of relative humidity (rh) on the infective potential of the isolate bb inra 297 of beauveria bassiana (bals.) vuillemin (deuteromycotina: hyphomycetes) against first instar nymphs of rhodnius prolixus stål. (hemiptera: reduviidae) was determined. fungus-treated insects were exposed to rhs ranged from 75 to 100% at 25 degrees c. results clearly showed a threshold of humidity at ca. 96% for high and rapid mortality. after initial exposure to increasing periods of 97% (4, 8, 16, 24, 36 a ...199910721518
effects of fluctuating moisture and temperature regimes on the infection potential of beauveria bassiana for rhodnius prolixus.the effect of both moisture and temperature on the infective potential of beauveria bassiana to the chagas' disease vector, rhodnius prolixus, was studied under fluctuating regimes. at constant 25 degrees c, contaminated first-instar nymphs exposed to increasing daily periods of initial exposure to 97% rh, followed by transfer to reduced humidity (43, 53, 75, and 86% rh), showed a significant reduction in mortality when the 97% rh exposure time declined from 12 to 8 h per day. the duration of di ...200010753596
comparative analysis of the binding of antibodies prepared against the insect spodoptera exigua and against the mycopathogen nomuraea rileyi.polyclonal antibodies were produced in mice against spodoptera exigua (beet armyworm) larval hemolymph and hemocytes and against cell wall surfaces of hyphal bodies and hyphae of the entomopathogenic hyphomycete nomuraea rileyi. in addition to exhibiting strong activity against their original antigenic substrates, all of the antibodies cross-react extensively with other substrates. the hemolymph antibody binds to hemocytes and vice versa, and both antibodies cross-react to the insect fat body ba ...200010772323
effect of entomopathogenic fungus, beauveria bassiana on larvae of three species of mosquitoes.beauveria bassiana, an entomopathogenic fungus, was evaluated for its potential against second and third instar larvae of culex quinquefasciatus, anopheles stephensi and aedes aegypti. conidiospores of this fungus were effective in causing infection leading to mortality of different larval instars. larvae of cx. quinquefasciatus were more susceptible to infection than an. stephensi and the second instar larvae of these two species were more susceptible than third instar larvae. larvae of ae. aeg ...199910783749
medical management of beauveria bassiana keratitis.to describe a case of beauveria bassiana keratitis and to discuss the management of this rare condition.200010832710
susceptibility of vespula vulgaris (hymenoptera: vespidae) to generalist entomopathogenic fungi and their potential for wasp control.the pathogenicity of vespula vulgaris wasp workers and larvae to a range of fungi was determined. all fungi were isolated in new zealand and included isolates from vespula, known generalist insect pathogens, and isolates generally nonpathogenic to insects. workers and larvae were highly susceptible to pathogenic isolates at high spore concentrations (>1.75 x 10(5) cfu/individual). eight isolates, two of metarhizium anisopliae, five of beauveria bassiana, and one of aspergillus flavus were pathog ...200010843831
biotransformation of cadinane sesquiterpenes by beauveria bassiana atcc 7159.incubation of cadina-4,10(15)-dien-3-one with beauveria bassiana atcc 7159 has resulted in the production of nine novel sesquiterpenes. these metabolites were identified as (4s)-cadin-10(15)-en-3-one, (4s)-3 alpha-hydroxycadin-10(15)-ene, (4r)-3 alpha-hydroxycadin-10(15)-ene, (4s)-3 beta-hydroxycadin-10(15)-ene, (4s)-3 beta-hydroxycadina-10(15),12(14)-diene, (4s)-13-hydroxycadin-10(15)-en-3-one, (4s)-12-hydroxycadin-10(15)-en-3-one, (4r)-3 beta, 14-dihydroxycadin-10(15)-ene and 3 alpha-hydroxyca ...200010846745
effects of biorational pesticides on four coccinellid species (coleoptera: coccinellidae) having potential as biological control agents in interiorscapes.the direct toxicity of insecticidal soap, horticultural oil, azatin, an extract from the neem tree containing azadiractin, and botainigard, a commercial formulation of the entomopathogenic fungus beauveria bassiana, was assessed on adults of four species of coccinellids--hippodamia convergens (guérin-ménéville), coleomegilla maculata (degeer), harmonia axyridis pallas, and cryptolaemus montrouzieri mulsant. all biorationals caused less mortality than a conventional pesticide, carbaryl (sevin). h ...200010902323
colonization of corn, zea mays, by the entomopathogenic fungus beauveria bassiana.light and electron microscopy were used to describe the mode of penetration by the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin into corn, zea mays l. after inoculation with a foliar spray of conidia, germinating hyphae grew randomly across the leaf surface. often a germ tube formed from a conidium and elongated only a short distance before terminating its growth. not all developing hyphae on the leaf surface penetrated the cuticle. however, when penetration did occur, the pene ...200010919808
prospects for the biological control of subterranean termites (isoptera: rhinotermitidae), with special reference to coptotermes formosanus.costs associated with subterranean termite damage and control are estimated to approach $2 billion annually in the united states alone. the formosan subterranean termite, coptotermes formosanus shiraki, is one of the more economically important subterranean species. in recent years, the shortcomings associated with conventional chemical control methods have prompted policymakers and scientists to evaluate the potential for biological control of subterranean termites (c. formosanus in particular) ...200010948359
characterization and carbon metabolism in fungi pathogenic to triatoma infestans, a chagas disease vector.the pathogenicity of metarhizium anisopliae (ma) and beauveria bassiana (bb) isolates against triatoma infestans, the major vector of chagas disease in argentina is reported. a 100% mortality was achieved with mean lethal times varying form 5.8 (ma6) to 7.7 (bb5) or 11.1 days (bb10). the fatty acid, hydrocarbon, and total lipid patterns were compared for glucose-grown and alkane-grown bb10 cultures. the alkane-grown cells showed a lipid pattern different from that of glucose-grown cells, with tr ...200011023748
effect of low viability on infectivity of beauveria bassiana conidia toward the silverleaf whitefly. 200011023752
effect of beauveria bassiana and metarhizium anisopliae (deuteromycetes) upon the coffee berry borer (coleoptera: scolytidae) under field conditions.the effect of three strains of the fungus beauveria bassiana (balsamo) vuillemin and two strains of metarhizium anisopliae (metschnikoff) sorokin upon the coffee berry borer, hypothenemus hampei (ferrari), was studied in three coffee farms at different altitudes (450-1,100 m above sea level) in soconusco, chiapas, mexico. the maximum average percentage mycosis varied according to altitude. at 450 m asl (el rincon) mycosis was 14.3% for b. bassiana and 6.3% for m. anisopliae; at 880 m asl (santa ...200011057711
laboratory evaluation of chemical-biological control of the rice weevil (sitophilus oryzae l.) in stored grains.the virulence of ten different fungal isolates of: beauveria bassiana, metarhizium anisopliae, verticillium lecanii and paecilomyces farinosus to the rice weevil sitophilus oryzae was tested. a fungal mix of the most efficient isolates, b. bassiana arsef 5500+m. anisopliae arsef 2974, which caused the highest mortality, was assayed in combination with fenitrothion at a concentration lower (3 ppm) than the normal 6 ppm. fungal inoculation of insects was done by spraying conidial suspensions of ea ...200011124371
pathogens associated with southwestern corn borers and southern corn stalk borers (lepidoptera: crambidae).a study was undertaken to isolate entomopathogens of southwestern corn borer, diatraea grandiosella dyer, and southern corn stalk borer, diatraea crambidoides (grote). field-collected diapausing larvae of southwestern corn borer (three sites in mississippi) and southern corn stalk borer (one site in north carolina), and a laboratory strain of d. grandiosella in the diapause state were maintained in a simulated winter followed by a simulated spring environnent. few larvae (< or = 6%) collected fr ...200011142290
selection of virulent isolates of entomopathogenic hyphomycetes against clavigralla tomentosicollis stål. and evaluation in cage experiment using three cowpea varieties.the virulence of 8 isolates of entomopathogenic hyphomycetes against adult and 5th instar nymph of clavigralla tomentosicollis was evaluated in the laboratory at 4 different concentrations of inoculum. at all concentrations, beauveria bassiana cpd 9 and metarhizium anisopliae cpd 5 caused the highest mortality in adult bug ranging from 58 to 97% and 53 to 100%, respectively at 7 days post inoculation. the same isolates had the shortest lt50 (3.5 and 4.1 days, respectively) and the lowest lc50 (1 ...199911189764
trials to control south american ticks with entomopathogenic fungi.ticks are parasites of great importance to man, domestic, and wild animals. in spite of the progress of the science, thus far, ticks are controlled mainly by chemical acaricides and when this technique is used in an indiscriminate way it can lead to ambiental pollution and development of tick resistance to acarcides. the studies developed in the universidade federal rural do rio de janeiro demonstrate that some isolates of the fungi metarhizium anisopliae and beauveria bassiana are pathogens for ...200011193672
laboratory and field evaluation of entomogenous fungi for tick control.aqueous and oil-based formulations of two entomogenous fungi, beauveria bassiana and metarhizium anisopliae were tested for their efficacy against the three major species of african ticks; namely, amblyomma variegatum, rhipicephalus apendiculatus, and boophilus decoloratus. both fungal species and formulations were observed to induce high mortalities, especially in the larvae. the oil-based formulation was found to be more effective than the aqueous formulation. monthly application of aqueous fo ...200011193673
biotransformation of diterpenes and diterpene derivatives by beauveria bassiana atcc 7159.the biohydroxylation of stemodin and stemodinone by beauveria bassiana atcc 7159 gave exclusively 2alpha,13,18-trihydroxystemodane and 13,18-dihydroxystemodan-2-one respectively. stemarin was converted to the novel 1beta,13,19-trihydroxystemarane and 13-hydroxystemarane-19-carboxylic acid. the synthesis and biotransformation of various derivatives of stemodin have also been studied.200111220282
effects of exogenous nutrients on conidial germination and virulence against the silverleaf whitefly for two hyphomycetes.exogenous protein and sugar sources were tested for their impact on conidial germination of two silverleaf whitefly (bemisia argentifolii) pathogens: beauveria bassiana and paecilomyces fumosoroseus. in liquid culture, sugars stimulated only 5-27% germination of b. bassiana and < or =11% germination of p. fumosoroseus, whereas, yeast extract or peptone stimulated 95-100% germination. in the absence of additional nutrients, agar alone stimulated approximately 50% germination. storing spores for d ...200111273689
evaluation of beauveria bassiana (hyphomycetes) strains as potential agents for control of triatoma infestans (hemiptera: reduviidae).chagas disease constitutes a major human health problem in most latin american countries. this endemic disease is transmitted by several species of triatomine bugs, the most important being triatoma infestans (klug). in this article, we report on the selection of strains of the entomopathogenic fungus beauveria bassiana (bals.) vuill. virulent to t. infestans for possible use as bioinsecticides. four strains of b. hassiana isolated from argentina (bb 1, 10, 25, and 65) were evaluated. to calcula ...200111296819
evaluation of synergistic interactions between the colorado potato beetle (coleoptera: chrysomelidae) pathogen beauveria bassiana and the insecticides, imidacloprid, and cyromazine.laboratory studies investigated the interaction between the fungal entomopathogen beauveria bassiana (balsamo) vuillemin and sublethal doses of the insecticides imidacloprid and cyromazine when applied to larvae of the colorado potato beetle, leptinotarsa decemlineata (say). when second instars were fed potato leaf discs treated with sublethal doses of imidacloprid and a range of doses of b. bassiana, a synergistic action was demonstrated. similar results were observed when larvae were sprayed d ...200111332824
antagonism between beauveria bassiana and imidacloprid when combined for bemisia argentifolii (homoptera: aleyrodidae) control.imidacloprid and the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin are both used to control the whitefly bemisia argentifolii bellows & perring. we tested whether the two control strategies acted additively, synergistically, or antagonistically when combined for whitefly control. we found antagonism in that b. bassiana inhibited the effectiveness of imidacloprid. when b. bassiana was combined with imidacloprid, insect response was either less than or similar to (depending on b. ...200111332825
desiccant dusts synergize the effect of beauveria bassiana (hyphomycetes: moniliales) on stored-grain beetles.diatomaceous earth (de) is a desiccant insecticide and most efficacious in low humidity. it acts on insect cuticle by absorbing lipids, and perhaps by cuticular abrasion. beauveria bassiana (balsamo) vuillemin, an entomopathogenic fungus, is most efficacious in high humidity and has a complex interaction with cuticular lipids. interaction between these materials may enhance insect control performance. assays with stored-grain beetles were conducted with b. bassiana at rates of 11, 33, 100, and 3 ...200111332827
entomogenous fungi as promising biopesticides for tick control.when ticks were sealed in nylon tetrapacks and infected with the entomogenous fungi, beauveria bassiana and metarizium anisopliae and maintained in potted grass in the field, the fungal oil formulations (10(9) conidia per ml) induced 100% mortality in larvae of rhipicephalus appendiculatus and amblyomma variegatum, whereas mortalities in nymphs varied between 80-100% and in adults 80-90%. the aqueous formulations (10(9) conidia per ml) induced mortalities of 40-50% and reductions in egg hatchabi ...200011354619
mortality of colorado potato beetle (leptinotarsa decemlineata) after sublethal stress with the cryiiia delta-endotoxin of bacillus thuringiensis and subsequent exposure to beauveria bassiana.acute or chronic sublethal exposure of colorado potato beetle larvae to the cryiiia delta-endotoxin of bacillus thuringiensis berliner did not significantly (p > 0.05) alter their subsequent susceptibility to beauveria bassiana (balsamo) vuillemin. during the period of exposure to b. bassiana there was continued mortality from previous exposure to delta-endotoxin, and b. bassiana also caused significant mortality. acute and chronic exposure to delta-endotoxin significantly prolonged larval devel ...200111356052
entomopathogenic fungi in flies associated with pastured cattle in denmark.cattle flies, including musca autumnalis, haematobia irritans, and hydrotaea irritans, are pests of pastured cattle. a 2-year study of the natural occurrence of entomopathogenic fungi in adult cattle flies and other flies associated with pastures showed that the four species included in the entomophthora muscae species complex (e. muscae sensu lato) caused high infection levels in several species of flies. however, only a few specimens of cattle flies were infected by e. muscae sensu stricto des ...200111356054
the effects of an entomopathogenic fungus, beauveria bassiana (balsamo) vuillemin (hyphomycetes), on prostephanus truncatus (horn) (col.: bostrichidae), sitophilus zeamais motschulsky (col.: curculionidae), and grain losses in stored maize in the benin republic.a fungal entomopathogen, beauveria bassiana, was used to treat maize ears placed in traditional grain stores against prostephanus truncatus in a field experiment conducted from september 1997 to march 1998 in the benin republic, west africa. treatments included oil-based spray with and without conidia, maize stored with and without the husk, and stores with and without artificial infestation. additional treated ears kept in insect-proof cages under field conditions were sampled weekly and expose ...200111356055
the effect of mode of exposure to beauveria bassiana on conidia acquisition and host mortality of colorado potato beetle, leptinotarsa decemlineata.the effects of the mode of exposure of second instar colorado potato beetles to beauveria bassiana on conidia acquisition and resulting mortality were investigated in laboratory studies. larvae sprayed directly with a b. bassiana condial suspension, larvae exposed to b. bassiana-treated foliage, and larvae both sprayed and exposed to treated foliage experienced 76, 34, and 77% mortality, respectively. the total number of conidia and the proportion of germinating conidia were measured over time f ...200111356057
quantitative assessment of biotic mortality factors of the european corn borer (lepidoptera: crambidae) in field corn.five treatments were used to exclude naturally occurring predators and parasitoids, based on body size and flight ability, to assess their effect on ostrinia nubilalis (hübner) populations on corn plaits. two initial o. nubilalis egg densities (one egg mass and three egg masses per plant) were assigned to each treatment. egg predation was higher in uncaged treatments than in caged treatments. flying insect predators, primarily coleomegilla maculata degeer (coleoptera: coccinellidae), reduced egg ...200111425014
effects of in vitro passage of beauveria bassiana on virulence to bemisia argentifolii. 200111437531
adult diabrotica spp. (coleoptera: chrysomelidae) infection at emergence with iindigenous beauveria bassiana (deuteromycotina: hyphomycetes). 200111437533
host plant effects on activity of the mitosporic fungi beauveria bassiana and paecilomyces fumosoroseus against two populations of bemisia whiteflies (homoptera: aleyrodidae).laboratory bioassays were conducted to determine the effect of host plant on mycosis in two geographically distinct populations of early 2nd-instar nymphs of bemisia argentifolii bellows & perring from the entomopathogenic fungi beauveria bassiana (balsamo) vuillemin and paecilomyces fumosoroseus (wize) brown & smith. mycosis in b. argentifolii nymphs varied according to the host plant on which the nymphs were reared but not according to the population. both populations of whiteflies reared on c ...200111502058
viability and stability of biological control agents on cotton and snap bean seeds.cotton and snap bean were selected for a multi-year, multi-state regional (south-eastern usa) research project to evaluate the efficacy of both commercial and experimental bacterial and fungal biological control agents for the management of damping-off diseases. the goal for this portion of the project was to determine the viability and stability of biological agents after application to seed. the biological seed treatments used included: (1) bacillaceae bacteria, (2) non-bacillaceae bacteria, ( ...200111517723
microbiological oxygenation of bridgehead azabicycloalkanes.a series of n-substituted bridgehead azabicycloalkanes has been prepared and examined as substrates for microbiological oxygenation using the fungi beauveria bassiana, rhizopus nigricans, aspergillus ochraceus, and rhizopus arrhizus. oxygenation using b. bassiana of n-tosyl-7-azabicyclo[2.2.1]heptane gave n-[p-(hydroxymethyl)benzenesulfonyl]-7-azabicyclo[2.2.1]heptane (56% yield), of n-(phenyloxycarbonyl)-7-azabicyclo[2.2.1]heptane gave the 2-endo-ol (56% yield, 51% ee), of n-boc-7-azabicyclo[2. ...199711671535
management of early-instar japanese beetle (coleoptera: searabaeidae) in field-grown nursery crops.numerous field studies were conducted in commercial nurseries in tennessee from 1996 through 1999 to evaluate chemical and biological treatments, application timing and rates, and method of application for control of early instars of japanese beetle, popillia japonica newman. insecticide treatments included bifenthrin, bendiocarb, chlorpyrifos, carbaryl, fipronil, halofenozide, imidacloprid, permethrin, tefluthrin, thiamethoxam, and trichlorfon. biological treatments included entomopathogenic ne ...200111681678
phylogeny of mitosporic entomopathogenic fungi: is the genus paecilomyces polyphyletic?we analyzed sequences of the divergent domain at the 5' end of the large subunit rrna gene from the mitosporic entomopathogenic fungi paecilomyces sp., paecilomyces fumosoroseus, paecilomyces farinosus, paecilomyces lilacinus, verticillium lecanii, verticillium psalliotae, beauveria bassiana, aschersonia sp., aschersonia placenta, ascomycetous cordyceps sp., and cordyceps militaris. phylogenetic analysis showed p. fumosorseus as the best characterized out of the analyzed species with the b. bass ...200111683463
sequencing of new beauverolides by high-performance liquid chromatography and mass spectrometry.mass spectrometry (ms) and tandem mass spectrometry (ms(n)) were used for the identification of beauverolides in the fermentation broth of beauveria bassiana and for evaluation of the purified fraction obtained by sublimation of beauverolides. besides being a new efficient route for purification of beauverolides, sublimation provided an enrichment of new minor lipophilic beauverolides of lower molecular weight from the original complex mycelial extract. the product ion collision-induced dissocia ...200111747104
pathogenicity of entomopathogenic fungi to different developmental stages of rhipicephalus sanguineus (acari: ixodidae).the pathogenicity of 4 species of entomopathogenic fungi (hyphomycetes species: beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, and paecilomyces fumosoroseus) to various developmental stages of rhipicephalus sanguineus (acari: ixodidae) ticks was compared under laboratory conditions. the most virulent isolate, m. anisopliae-108, caused 92-96% mortality to unfed larvae and nymphs on day 7 postinfection (pi) and 100% mortality to unfed adults and engorged females on day 21 pi. ...200111780821
biotransformation of phenylurea herbicides by a soil bacterial strain, arthrobacter sp. n2: structure, ecotoxicity and fate of diuron metabolite with soil fungi.in order to assess the influence of the aromatic substitution on the ability of a soil bacterial strain, arthrobacter sp. n2, to degrade phenylurea herbicides, biotransformation assays were performed in mineral medium with resting cells of this soil bacterial strain on three phenylurea herbicides (diuron, chlorotoluron and isoproturon). each herbicide considered, led to the formation of only one metabolite detected by hplc analysis. after isolation, the metabolites were identified by nmr and ms, ...200211838430
allelic variation of a beauveria bassiana (ascomycota: hypocreales) minisatellite is independent of host range and geographic origin.the minisatellite locus, bbmin1, was isolated from a partial beauveria bassiana genomic library that consisted of poly(ga) flanked inserts. polymerase chain reaction (pcr) of the bbmin1 repeat demonstrated allele size variation among 95 b. bassiana isolates. amplification was also observed from single isolates of beauveria amorpha, beauveria brongniartii, and beauveria caledonica. eight alleles were identified at the haploid locus, where repeat number fluctuated between one and fourteen. amova a ...200211908654
bulbs mycoflora and their relation with three stored product mites.the distribution of moulds on stored and field onion and garlic plants infested by bulb mites in assiut area (egypt) was studied using pda medium at 28 degrees c. among 40 host samples and the three mite species tested no significant difference was noted in the contamination by moulds. a total of 20 species appertaining to 11 genera were identified from the tested mites and their habitats. the predominant moulds on all samples were "storage moulds" from the genera aspergillus (a. niger, a. versi ...200211913764
beauveria bassiana as a pathogen of the mexican fruit fly (diptera: tephritidae) under laboratory conditions.bioassays were carried out under controlled conditions (27 +/- 2 degrees c, 80 +/- 5% rh, and a photoperiod of 12:12 [l:d] h) to evaluate the effect of eight strains of the entomopathogenic fungus beauveria bassiana upon larvae, pupae, and adult females of the mexican fruit fly, anastrepha ludens (loew). mortality of the immature stages was low, 2-8% in larvae and 0% in pupae. however, very high levels of mortality were obtained for adult flies, with values of 100, 98, and 98% for the strains bb ...200211942762
selectivity of biohydroxylation with beauveria bassiana of trans-2-fluorocycloalkyl n-phenylcarbamates.biohydroxylation with beauveria bassiana of racemates and the pure enantiomers of trans-2-fluorocyclohexyl- 3 and trans-2-fluorocycloheptyl n-phenylcarbamates 6 were investigated and compared with results found for the corresponding nonfluorinated parent compounds. in all cases, mixtures of diastereomeric products hydroxylated in the 4-position were isolated, besides products of p-hydroxylation of the aromatic ring and succeeding compounds derived from these primary reaction products. the regios ...200211975562
transformation system of beauveria bassiana and metarhizium anisopliae using nitrate reductase gene of aspergillus nidulans.an heterologous transformation system for entomopathogenic fungi b. bassiana and m. anisopliae was developed based on the use of a. nidulans nitrate reductase gene (niad). b. bassiana and m. anisopliae niad stable mutants were selected by treatment of protoplast with ethane methane sulphonate (ems) and regenerated on chlorate medium. the cloned gene was capable of transforming b. bassiana and m. anisopliae at a frequency of 5.8 to 20 transformants per microg of dna. most of them were mitotically ...200112019757
conidium production by insect pathogenic fungi on commercially available agars.conidium production by three species of insect pathogenic fungi, metarhizium anisopliae, beauveria bassiana and verticillium lecanii, was assessed on various depths and types of commercially available agars.200212081554
[cloning and characterization of cuticle degrading enzyme cdep-1 from beauveria bassiana].beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. a cdna library was constructed using mrna from mycelia of beauveria bassiana grown on cuticle/chitin cultures. a cdna clone of the protease, designated cdep-1, was isolated from cdna library. cdep-1 contained an 1,134 bp orf that predicted a protein of 377 amino acids with m(r) = 38,616 and pi = 8.302. the amino acid sequence of the gene shows 57 ...200212182086
transformation of cinoxacin by beauveria bassiana.the ability of the fungus beauveria bassiana atcc 7159 to transform the antibacterial agent cinoxacin was investigated. cultures in sucrose-peptone broth were dosed with cinoxacin, grown for 20 days, and then extracted with ethyl acetate. two metabolites were detected and purified by high-performance liquid chromatography. the major metabolite was identified by mass and proton nuclear magnetic resonance spectra as 1-ethyl-1,4-dihydro-3-(hydroxymethyl)[1,3]dioxolo[4,5-g]cinnolin-4-one and the min ...200212204384
isolation of beauveria bassiana and metarhizium anisopliae var. anisopliae from boophilus microplus tick (canestrini, 1887), in rio de janeiro state, brazil.the objective of this work were to isolate and identify strains of entomopathogenic fungi from ingurgitated female boophilus microplus ticks, collected from the soil in the municipality of paracambi, rio de janeiro state, brazil. the ingurgitated females were inoculated in the selective medium oat dodine agar (oda), where 49 colonies of beauveria bassiana (71%) and 20 of metarhizium anisopliae var. anisopliae (29%) were isolated. these isolated strains characterize for the first time in brazil t ...200212206323
eicosanoids mediate manduca sexta cellular response to the fungal pathogen beauveria bassiana: a role for the lipoxygenase pathway.many studies have documented the involvement of eicosanoids in insect cellular immune responses to bacteria. the use of the fungal pathogen beauveria bassiana as a nodulation elicitor, with inhibition of phospholipase a(2) by dexamethasone, extends the principle to fungi. this study also provides the first evidence of involvement of the lipoxygenase (lox) pathway rather than the cyclooxygenase (cox) pathway in synthesis of the nodulation mediating eicosanoid(s). the lox product, 5(s)-hydroperoxy ...200212210960
[time-specific infection rate of beauveria bassiana on myzus persicae after topical inoculation of conidial suspension].conidial suspension(5 x 10(6) conidia.ml-1) of the entomopathogenic fungus, beauveria bassiana bbsg8702 was used to inoculate newly emerged apterous adults of the green peach aphid (myzus persicae) on detached cabbage leaves in order to measure time-specific infection rate of the fungal agent. the aphids of conidia attached were disinfected using 0.2% chlorothalonil solution at intervals of 8-56 h after inoculation and then maintained at 10 and 20 degrees c for daily observation. there was a sig ...200212216397
field-based evaluation of biopesticide impacts on native biodiversity: malagasy coleoptera and anti-locust entomopathogenic fungi.a community of 225 species of coleoptera was used as a surrogate to evaluate nontarget effects of entomopathogenic fungi under development as biopesticides for use against the malagasy migratory locust locusta migratoria capito saussure (orthoptera: acrididae). evaluation of a standard chemical treatment of fenitrothion + esfenvalerate, two indigenous isolates of metarhiziumflavoviride gams & roszsypol (sp3 and sp9), and an indigenous isolate of beauveria bassiana (balsamo) vuillemin (sp16) agai ...200212216803
pathogenicity of beauveria bassiana, metarhizium anisopliae (deuteromycotina: hyphomycetes), and other entomopathogenic fungi against lygus lineolaris (hemiptera: miridae).the pathogenicity of 32 fungal isolates from the genera of beauveria, verticillium, paecilomyces, metarhizium, mariannaea, and hirsutella to second-instar tarnished plant bug, lygus lineolaris (palisot de beauvois), was tested under laboratory conditions. these isolates originated from various insect hosts and substrates from france, denmark, austria, italy, turkey, syria, and the united states. a single exposure concentration (1 x 10(7) conidia/ ml) assay for each isolate was first conducted by ...200212216806
[variation in the viability and the metabolism of internally reserved nutrients of beauveria bassiana conidia during storage at different regimes of water content and temperature].while the fresh powder (with water content of 58.9 +/- 1.6%) and vacuum-freeze dried (vfd) one (with water content of 7.4 +/- 0.9%) of beauveria bassiana sgbb8702 conidia were stored at 4 degrees c and 20 degrees c, their viability and contents of internally reserved saccharides and proteins were examined at 5 days intervals. it was found during one-month storage that the water content and temperature interactively affected the levels of conidia viability and the contents of the reserved sacchar ...200212222050
nuclear small subunit rrna group i intron variation among beauveria spp provide tools for strain identification and evidence of horizontal transfer.an optional group i intron was characterized at a single insertion point in nuclear small subunit rrna (nussu rrna) genes of the imperfect entomopathogenic fungi, beauveria bassiana and b. brongniartii. insertion points were conserved among nussu rrna genes from 35 beauveria isolates. pcr-rflp and dna sequencing identified 12 group i intron variants and were applied to the identification of strains isolated from insect hosts. alignment of 383-404-nt subgroup ib3 group i introns indicated that fo ...200212228811
molecular studies of co-formulated strains of the entomopathogenic fungus, beauveria bassiana.a 28s rdna intron was used as a molecular marker to distinguish between two single spore strains of beauveria bassiana, bb123 and bb151. when co-formulated and assayed against larvae of galleria mellonella these strains exhibited no synergistic increase in virulence, rather bb123 usually dominated. this study shows that the success of any strain to infect galleria is dependent on the dose and method of inoculation (injection versus immersion). the result of co-formulated strains grown on solid c ...200212234539
natural enemies of coelomera lanio (coleoptera: chrysomelidae) in the region of viçosa, minas gerais, brazil.coelomera lanio (coleoptera: chrysomelidae) is the most important defoliator of cecropia trees. natural enemies of c. lanio collected in the region of viçosa, state of minas gerais, brazil were identified on field observations in a forest fragment and on laboratory analyses. individuals of c. lanio were not found on cecropia pachystachya trees colonized by azteca mülleri (hymenoptera: formicidae). the majority of the egg masses of c. lanio collected in the field were found to be parasitised by a ...200212298235
carpophilus freemani (coleoptera: nitidulidae) as a vector of beauveria bassiana. 200212384085
continuous production of extracellular l-glutaminase by ca-alginate-immobilized marine beauveria bassiana btmf s-10 in packed-bed reactor.l-glutamine amidohydrolase (l-glutaminase, ec 3.5.1.2) is a therapeutically and industrially important enzyme. because it is a potent antileukemic agent and a flavor-enhancing agent used in the food industry, many researchers have focused their attention on l-glutaminase. in this article, we report the continuous production of extracellular l-glutaminase by the marine fungus beauveria bassiana btmf s-10 in a packed-bed reactor. parameters influencing bead production and performance under batch m ...200212396112
group-i intron containing a putative homing endonuclease gene in the small subunit ribosomal dna of beauveria bassiana ifo 31676. 200212411610
genetic groups of the insect-pathogenic fungus beauveria bassiana are associated with habitat and thermal growth preferences.a persistent paradigm in insect pathology is one that relates the insect host to certain genetic groups of insect-pathogenic fungi. this paradigm assumes that the genotype of an insect-pathogenic fungus coevolves with a certain taxon of insect host that it infects. the insect-pathogenic fungus beauveria bassiana shows a wide host range and is considered to be a facultative insect pathogen. in this study, a population genetics analysis of b. bassiana from forested and agricultural habitats as wel ...200212420176
beauveria bassiana yeast phase on agar medium and its pathogenicity against diatraea saccharalis (lepidoptera: crambidae) and tetranychus urticae (acari: tetranychidae).beauveria bassiana colonizes insect hosts initially through a yeast phase, which is common in some artificial liquid cultures, but not reported on artificial solid media. we describe a yeast-like phase for b. bassiana isolate 447 (atcc 20872) on macconkey agar and its virulence toward diatraea saccharalis and tetranychus urticae. the yeast-like cells of b. bassiana developed by budding from germinating conidia after 24-h incubation. cells were typically 5-10 microm and fungal colonies were initi ...200212445790
sporulation of metarhizium anisopliae and beauveria bassiana on coptotermes formosanus and in vitro.the sporulation of 22 total isolates of metarhizium anisopliae and beauveria bassiana was quantified on cadavers of the formosan subterranean termite, coptotermes formosanus. conidial production increased significantly over 11 days post-death. effects of isolates of m. anisopliae and b. bassiana on in vivo sporulation were significant. although the overall effects of fungal species on in vivo sporulation were not significant, the interactions between fungal species and certain times post-death w ...200212445791
the potential for enhanced fungicide resistance in beauveria bassiana through strain discovery and artificial selection.our objectives were to determine the (1) natural variation in fungicide resistance among beauveria bassiana strains, (2) potential to increase fungicide resistance in b. bassiana through artificial selection, and (3) stability of virulence in selected b. bassiana strains. fungicides included dodine, fenbuconazole, and triphenyltin hydroxide, which are commonly used in pecan and other horticultural crops. comparison of seven b. bassiana strains indicated some are substantially more resistant to f ...200212445792
pathogenicity of two entomopathogenic hyphomycetes, beauveria bassiana and metarhizium anisopliae, to the housefly musca domestica l.the pathogenicity of three isolates of metarhizium anisopliae and two isolates beauveria bassiana originating from different insect species and localities was tested against housefly larvae. only one isolate of m. anisopliae (ma23) and the two isolates of b. bassiana (bb2, bb108) significantly (p<0.05) reduced the percentage of emerging adults. at the highest tested concentration 10(7) spores/ml. the reduction in the percentage of adult emergence was 93.8%. 61.3% and 49% for bb2. ma23 and bb108. ...200212512811
assessment of health and growth of ring-necked pheasants following consumption of infected insects or conidia of entomopathogenic fungi, metarhizium anisopliae var. acridum and beauveria bassiana, from madagascar and north america.isolates of two fungi (beauveria bassiana and metarhizium anisopliae var. acridum) from madagascar are being developed for control of grasshoppers and locusts, as part of a search for alternatives to environmentally detrimental chemical insecticides. the probable effects of these entomopathogens on nontarget species must be determined before operational use. birds may become exposed to these fungi either directly, by consuming spores deposited on their food items, or secondarily, by consuming gr ...200212515592
[genetical analysis on cross incubation of beauveria bassiana].heterokaryon of beauveria bassiana was formed during the cross incubation of two vegetative compatible strains with genetic markers of actidione resistance and 34 degrees c tolerance. the chromosome(s) or its fragment successive losses, recombination and segreation led to haploidization during the conidia formation period. after at least 4 generations of parasexual cycle, the genetic character of heterokayon could get to relative stable. genetic marker and rapd analysis indicated that the combin ...200012548769
[reliability of extracellular protease and lipase activities of beauveria bassiana isolates used as their virulence indices].the extracellular protease and lipase activities of 17 beauveria bassiana isolates from different hosts and countries were evaluated for the reliability for the indices of their virulence to the migratory grasshopper, melanoplus sanguinipes. virulence assay of each isolate included about 30 10-d-old grasshoppers receiving topical inoculation with the suspension of 10(7) conidia/ml. in the assays of the enzymes, n-succinyl-ala-ala-pro-phe-p-nitroanilide and p-nitrophenyl palmitate were used as a ...199812548926
[isolation, detection toxicity and structure of toxin from beauveria bassiana].in this experiment, the crude toxin from the metabolite of beauveria bassiana was isolated and its toxicity was detected. the result shows that indicates toxin has toxicity on larva of aedesalbopictus obvious. though it with low toxicity to larva of heliothis zea when swallowed, but has high toxicity when injected into the blood. for bacteria it only inhibites g+. when it applied to cultured cells of spodoptera frugiperda sf-21, the regression equation of toxicity is y = 2.03 + 2.39x. the half l ...199812548927
laboratory evaluation of temperature effects on the germination and growth of entomopathogenic fungi and on their pathogenicity to two aphid species.as part of an approach to select potential mycoinsecticides for aphid biocontrol, we investigated the effects of temperature on the growth, germination and pathogenicity of some hyphomycete fungi. commercially available mycoinsecticides (based on beauveria bassiana (balsamo) vuillemin and verticillium lecanii (zimmermann) viegas) and other isolates of b bassiana, v lecanii, metarhizium anisopliae (metschnikoff) sorokin and paecilomyces fumosoroseus (wize) brown & smith were evaluated. the rate o ...200312587869
strain-specific detection of introduced beauveria bassiana in agricultural fields by use of sequence-characterized amplified region markers.field studies on the efficacy and persistence of an introduced strain of beauveria bassiana for insect control require detection assays to differentiate the non-native strain from indigenous populations. in this study we developed strain-specific molecular markers based on polymerase chain reaction amplification of sequence-characterized amplified regions (scar) in combination with dilution plating on semi-selective medium to detect and estimate density of propagules of a commercial strain of b. ...200312623307
[population dynamics of beauveria bassiana in masson pine plantation ecosystem].annual dynamics of beauveria bassiana in 12 experimental plots of masson pine plantation inoculated at different doses and frequencies was investigated. the results showed that inoculum density of b. bassiana in the plantation was correlative to released inoculum in initial stage after b. bassiana released. in this period, the amount of inoculum densities at different niches of the plantation was in order of litter layer > crown layer > soil layer. four months later, inoculum densities in x-plot ...200212624985
biotransformations of the cardiovascular drugs mexrenone and canrenone.microbial transformation studies of the cardiovascular drugs mexrenone (1) and canrenone (2) were conducted. thirty-nine biotransformations of mexrenone and 84 biotransformations of canrenone were analyzed. metabolism of the substrate was observed in the majority of these cases. several monohydroxylated derivatives were detected by hplc-ms-uv and subsequently identified. two new mexrenone derivatives, 11alpha- (3) and 12beta-hydroxymexrenone (4), and the known metabolite 6beta-hydroxymexrenone ( ...200312662091
characterization of beauveria bassiana and metarhizium anisopliae isolates for management of tarnished plant bug, lygus lineolaris (hemiptera: miridae).selected morphological and physiological characteristics of four beauveria bassiana (balsamo) vuillemin isolates and one metarhizium anisopliae (metschnikoff) sorokin isolate, which are highly pathogenic to lygus lineolaris (palisot de beauvois) (hemiptera: miridae), were determined. there were significant differences in conidial size, viability, spore production, speed of germination, relative hyphal growth, and temperature sensitivity. spore viability after incubation for 24h at 20 degrees c r ...200312676549
starvation induced stress and the susceptibility of the colorado potato beetle, leptinotarsa decemlineata, to infection by beauveria bassiana.starvation of second instar colorado potato beetle larvae for 24h immediately after treatment with beauveria bassiana conidia increased susceptibility to the pathogen and subsequent sporulation of cadavers but decreased time to larval death. in feeding studies, b. bassiana-treatment had no effect on subsequent larval development, and mortality occurred 5-6 days after treatment. twenty-four hours of starvation alone retarded subsequent larval development but did not affect mortality. mortality of ...200312788282
isolation and characterization of a carboxylic transport protein jen1 and its promoter from metarhizium anisopliae.based on the flanking sequence of t-dna of a t-dna insertion mutant of beauveria bassiana, t12, the full length cdna of carboxylic transport protein, designated majen1, was cloned from metarhizium anisopliae. majen1 is 1,695 bp long and contained a 1,524 bp orf which predicted a protein of 508 amino acid. the amino acid sequence of the gene showed 69% and 31% identity to the carboxylic transport protein of neurospore crassa and saccharomyces cerevisiae, respectively. the genome sequence, gmajen1 ...200312812095
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