Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cutaneous infection caused by fusarium solani and fusarium oxysporum. | 1987 | 3325538 | |
| acute bovine pulmonary emphysema caused by the fungus fusarium semitectum. | acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse. | 1988 | 3388752 |
| two linear plasmids in mitochondria of fusarium solani f. sp. cucurbitae. | two linear plasmid-like dnas designated pfsc1 (9.2 kbp) and pfsc2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus fusarium solani f. sp. cucurbitae race 1. the plasmids were maternally inherited and copurified with mitochondrial dna obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. the plasmids did not share extensive sequence similarity. no homology was detected between either plasmid and the nuclear or mitochondrial genome w ... | 1988 | 3399553 |
| granulomatous peritonitis in fish associated with fusarium solani. | 1987 | 3438995 | |
| disseminated hyalohyphomycosis in a leukemic patient. | a 69-year-old man with acute myeloblastic leukemia was admitted to the hospital for induction chemotherapy. a complication caused by a disseminated mycotic infection occurred while the neutropenic patient was maintained on a course of cytotoxic drugs, antibiotics, and corticosteroid therapy. the causal isolate was identified as fusarium solani on the basis of its colonial and microscopic morphology. this fungus developed in the patient's tissue in the form of hyaline, branched, septate hyphae. t ... | 1986 | 3464232 |
| corneal biopsy in the diagnosis of keratomycosis. | in two patients, a 55-year-old man and a 49-year-old man, who had fungal keratitis initially undiagnosed by corneal scrapings the condition was successfully diagnosed by corneal biopsy. we compared corneal biopsy specimens and corneal scraping in the diagnosis of keratomycosis in rabbits with experimental bilateral fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. corneal scrapings disclosed three specimens (30%) positive for candida, five (50%) for fusariu ... | 1986 | 3485380 |
| rapid visualization of three common fungi using fluorescein-conjugated lectins. | the feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. using a panel of fluorescein-conjugated lectins, candida albicans, aspergillus fumigatus, and fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed histopathologic specimens taken from experimental mycoses. additionally, aspergillus and fusarium were consistently differenti ... | 1986 | 3514531 |
| fungemia due to fusarium solani in an immunocompromised child. | 1986 | 3523454 | |
| comparison of the pathogenicities of fusarium solani and candida albicans in the rabbit cornea. | the pathogenicity of f. solani was compared with that of candida albicans in experimental keratitis in rabbits. f. solani was inoculated into the right eye of each rabbit, and c. albicans into the left eye. the corneal lesions in both eyes were examined by slit lamp every day for 3 weeks and the severity of infections compared clinically and histopathologically. f. solani produced significantly more severe clinical infection in the cornea, compared to c. albicans. histopathologically, f. solani ... | 1986 | 3537261 |
| microbiological diagnosis of suppurative keratitis in bangladesh. | experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ... | 1987 | 3555609 |
| direct examination vs culture of biopsy specimens for the diagnosis of keratomycosis. | in two patients with fungal keratitis, direct examination of corneal biopsy specimens showed positive fungal elements, but cultures of biopsy specimens failed to disclose fungal growth. we compared the value of direct examination and culture of biopsy specimens in the diagnosis of keratomycosis in rabbits with experimental fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. cultures disclosed seven specimens (70%) positive for candida and eight (80%) for fusa ... | 1987 | 3578461 |
| antibiotic activity of the pyrenocines. | pyrenocine a, a phytotoxin produced by pyrenochaeta terrestris (hansen) gorenz, walker and larson, possesses general antibiotic activity against plants, fungi, and bacteria. effective doses for 50% inhibition (ed50s) are 4 micrograms/ml for onion seedling elongation; 14, 20, 20, and 25 micrograms/ml for the germination of asexual spores of fusarium oxysporum f. sp. cepae, fusarium solani f. sp. pisi, mucor hiemalis, and rhizopus stolonifer, respectively. pyrenocine a also inhibits the linear myc ... | 1987 | 3594310 |
| enzymatic hydrolysis of diethylpyrocarbonate, a commonly used histidine modifying agent, by esterases. | diethylpyrocarbonate, a reagent commonly used to modify active site histidines in enzymes, was found to be hydrolyzed by several esterases. two of these, cutinase, a typical serine esterase from the fungus fusarium solani pisi, and thioesterase b from the uropygial gland of the mallard duck anus platyrhynchus, hydrolyzed diethylpyrocarbonate so rapidly that histidine modification could not be detected except when the enzymic activity was inhibited by diisopropylfluorophosphate treatment or by th ... | 1987 | 3595987 |
| fast method for two-dimensional electrophoresis of proteins from biological samples. | the method for two-dimensional gel electrophoresis of j. klose and m. feller [(1981) electrophoresis 2, 12-24] has been simplified by reducing the thickness of the gels from 3.5 to 1.1 mm for isoelectric focusing gels and from 3.5 to 0.84 mm for sodium dodecyl sulfate slab gels. thin gels need less reagents and smaller sample volumes. cooling of the thin gels during electrophoresis is more effective, which allows the use of higher electric power. therefore, less time is required for an electroph ... | 1987 | 3674367 |
| the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases. | two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ... | 1986 | 3707549 |
| fusarium solani: prevalence of skin reactivity and antigenic allergenic analysis. | the prevalence of fusarium solani reactivity in atopic individuals with symptoms of mold allergy was assessed with skin test and rast. in addition, f. solani preparations were evaluated for antigenic/allergenic activity. atopic individuals tested, 24.5% (n = 69), had positive skin reactions to a phosphate-buffered saline extract of f. solani, and these responses were statistically correlated with rast results. immunoelectrophoretic techniques demonstrated that this extract was antigenic in rabbi ... | 1986 | 3711551 |
| the pathogenicities of cylindrocarpon tonkinense and fusarium solani in the rabbit cornea. | the pathogenicity of cylindrocarpon tonkinense in the cornea was evaluated and compared with that of fusarium solani in rabbits. f. solani was inoculated into the right eyes of 14 rabbits and c. tonkinense was into the left eyes of same rabbits. the corneal lesions of both eyes were examined carefully by slit lamp every day for three weeks and the severity of infections were compared each other. for histopathologic study, several eyes were enucleated periodically. c. tonkinense has a pathogenici ... | 1986 | 3762655 |
| isolation of a fusarium solani mutant reduced in cutinase activity and virulence. | fusarium solani isolate t-8 produces an extracellular enzyme, cutinase, which catalyzes the degradation of cutin in the plant cuticle. cutinase activity can be measured by the hydrolysis of either the artifical substrate, p-nitrophenylbutyrate (pnb), or radioactive cutin containing [14c]palmitic acid. in the present study, the culture filtrate contained basal levels of cutinase when t-8 was grown on acetate as a sole source of carbon. after mutagenesis, a cutinase-defective mutant (pnb-1) was id ... | 1986 | 3782031 |
| high field and 2d-nmr studies with the aporphine alkaloid glaucine. | the aporphine alkaloid glaucine (1) was examined by comparison of the high field (600 mhz) 1h-nmr spectra of 1 vs. racemic 6a,7,7-trideutereoglaucine (4,5), by computer-simulated 1h-nmr spectra at 600 mhz, by using decoupled proton spectra, and two-dimensional cosy and hetcor experiments with 1 at 500 and 360 mhz, respectively, and using high field (90 mhz) 13c-nmr of s-(+)-glaucine (1). emphasis was placed on the resolution of the chemical shifts and coupling constants for the h-4 alpha, h-4 be ... | 1986 | 3783155 |
| calcofluor and ink-potassium hydroxide preparations for identifying fungi. | calcofluor and ink-potassium hydroxide preparations identified fusarium solani, aspergillus fumigatus, and candida albicans, the three most common ocular fungal pathogens, in scrapings, biopsy specimens, and tissue sections of corneal mycotic infections in rabbits. these stains also identified fungal organisms in specimens from four human patients with keratomycoses. neither procedure requires more than a few minutes to perform or extensive training or experience to interpret. the specimen stain ... | 1985 | 4061554 |
| solaniol, a toxic metabolite of fusarium solani. | fusarium solani m-1-1 isolated from moldy bean hulls produces t-2 toxin, diacetoxyscirpenol, and a new toxic trichothecene, solaniol, in czapek-dox-peptone medium. | 1971 | 4331775 |
| fusarium solani infection during treatment for acute leukemia. | 1973 | 4518930 | |
| fusarium solani keratitis treated with natamycin (pimaricin): eighteen consecutive cases. | 1972 | 4537911 | |
| toxicological approaches to the metabolites of fusaria. iv. microbial survey on "bean-hulls poisoning of horses" with the isolation of toxic trichothecenes, neosolaniol and t-2 toxin of fusarium solani m-1-1. | 1972 | 4538152 | |
| observations on strains of fusarium solani, f. oxysporum and candida parapsilosis from ulcerated legs. | 1972 | 4553714 | |
| distribution of steroid 1-dehydrogenation and side-chain degradation enzymes in the spores of fusarium solani: causes of metabolic lag and carbohydrate independence. | the spores of a strain of fusarium solani 1-dehydrogenate ring a and cleave the 17beta-acetyl side chain of 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-hydroxyprogesterone) to give 17alpha-hydroxypregna-1,4-diene-3,20-dione (the 1-dehydro analogue) and little androsta-1,4-diene-3,4-diene-3,17-dione (androstadienedione). a 4-h lag period is observed in the course of metabolism, and there are no requirements for external additives. exoenzymes or surface enzymes bound to the cell outside the pla ... | 1973 | 4699223 |
| modification of the exogenous carbon and nitrogen requirements for chlamydospore germination of fusarium solani by contact with soil. | 1973 | 4752344 | |
| steroid 1-dehydrogenation and side-chain degradation enzymes in the life cycle of fusarium solani. | 1973 | 4756113 | |
| effect of aureofungin on seedling mortality and growth of two varieties of sesame with seed-borne infection of fusarium solani. | 1973 | 4763647 | |
| depolymerization of a hydroxy fatty acid biopolymer, cutin, by an extracellular enzyme from fusarium solani f. pisi: isolation and some properties of the enzyme. | 1973 | 4784475 | |
| corneal and intra-ocular infection due to fusarium solani. | 1970 | 4925294 | |
| chlamydospore induction in pure culture in fusarium solani. | 1971 | 4935359 | |
| reduction of the 20-carbonyl group of c-21 steroids by spores of fusarium solani and other microorganisms. i. side-chain degradation, epoxide cleavage, and substrate specificity. | the spores of fusarium solani reduced the c(2)-carbonyl group, 1-dehydrogenated ring "a" and cleaved the side chain of 16alpha, 17alpha-oxidopregn-4-ene-3, 20-dione (16alpha, 17alpha-oxidoprogesterone)(i) to give the following products: 20alpha-hydroxy-16alpha, 17alpha-oxidopregn-4-en-3-one(ii); 20alpha-hydroxy-16alpha, 17alpha-oxidopregna-1, 4-dien-3-one(iii); 16alpha-hydroxy-17a-oxa-androsta-1, 4-diene-3, 17-dione (16alpha-hydroxy-1-dehydrotestololactone)(iv); and 16alpha, 17beta-dihydroxy-and ... | 1972 | 5021973 |
| the cellulase of fusarium solani. purification and specificity of the -(1-4)-glucanase and the -d-glucosidase components. | 1. cell-free culture filtrates of the fungus fusarium solani were examined for homogeneity with respect to beta-d-glucosidase and c(x) activities. 2. o-nitrophenyl beta-d-glucoside and cellobiose were both used as substrates for beta-d-glucosidase activity. 3. no evidence for the non-identity of nitrophenyl beta-d-glucosidase and cellobiase activities could be found, either by heat treatment, gel filtration on sephadex g-100 or by isoelectric focusing. 4. the beta-d-glucosidase component was als ... | 1971 | 5119766 |
| proteins and catalase isoenzymes from fusarium solani and their taxonomic significance. | 1967 | 5340501 | |
| the cellulase of fusarium solani. resolution of the enzyme complex. | 1. culture filtrates from fusarium solani were fractionated by ion-exchange chromatography on deae-sephadex, followed by gel chromatography on sephadex g-100, into a c(1) component, a c(x) component (cm-cellulase) and a beta-glucosidase (cellobiase) component. 2. the individual components showed little capacity for the solubilization of cotton fibre (cellulase activity), but when recombined in their original proportions 81% of the original cellulase activity was recovered. 3. the c(1) components ... | 1969 | 5389535 |
| induction of chlamydospore formation in fusarium solani by soil bacteria. | 1970 | 5436564 | |
| herbicide transformation. i. studies with whole cells of fusarium solani. | a strain of fusarium solani isolated from soil by enrichment techniques used propanil (3', 4'-dichloropropionanilide) as a sole source of organic carbon and energy for growth in pure culture. the primary product of the transformation of propanil by f. solani was isolated and identified as 3,4-dichloroaniline (dca). this compound accumulated in the medium to a level (80 mug/ml) which stopped further herbicide utilization. herbicide utilization by f. solani was influenced by various environmental ... | 1970 | 5437305 |
| herbicide transformation. ii. studies with an acylamidase of fusarium solani. | replacement cultures liberated 3,4-dichloroaniline (dca) from 3,4-dichloropropionanilide (propanil). the kinetics of the conversion suggest a requirement for de novo enzyme synthesis, but the system was not influenced by chloramphenicol or puromycin. enzyme activity was detected when acetanilide (k(m) = 0.195 mm) was used to replace propanil as substrate. fungal acylamidase (e.c. 3.5.1., an aryl acylamine amidohydrolase) was concentrated by salt precipitation and characterized. the fusarium sola ... | 1970 | 5437306 |
| carbon and nitrogen requirements for macroconidial germination of fusarium solani: dependence on conidial density. | 1970 | 5484061 | |
| chlamydospore development in the absence of protein synthesis in fusarium solani. | 1970 | 5485812 | |
| exogenous carbon and nitrogen requirements for chlamydospore germination by fusarium solani: dependence on spore density. | 1970 | 5521394 | |
| [plants affecting the phytopathogenic fungi. iv. fusarium solani f. pisi (jones) snyd. et hans]. | 1970 | 5535932 | |
| ribosomal competence and spore germination in fusarium solani. | extracts prepared from macroconidia of fusarium solani f. sp. phaseoli are capable, under defined conditions, of incorporating phenylalanine into polypeptide with exogenous polyuridylic acid as messenger. extracts from ungerminated and germinated spores have approximately the same activity. with endogenous template, leucine incorporation occurs, but in this reaction extracts from germinated spores have about 10 times more activity than do those from ungerminated spores. it is suggested that the ... | 1971 | 5573727 |
| effects of tannins on spore germination and growth of fusarium solani f. phaseoli and verticillium albo-atrum. | 1967 | 5625600 | |
| hydrolytic and trans-eliminative degradation of pectic substances by extracellular enzymes of fusarium solani f. phaseoli. | 1966 | 5901783 | |
| susceptibility and resistance of several fungi to microbial lysis. | potgieter, h. j. (cornell university, ithaca, n.y.), and m. alexander. susceptibility and resistance of several fungi to microbial lysis. j. bacteriol. 91:1526-1532. 1966.-strains of streptomyces, nocardia, and pseudomonas capable of lysing hyphae of fusarium solani or neurospora crassa were obtained by selective culture, but attempts to isolate an organism lysing rhizoctonia solani failed. when provided with f. solani or n. crassa as carbon sources, the actinomycetes tested produced beta-(1 --> ... | 1966 | 5929777 |
| development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues. | we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ... | 1983 | 6195179 |
| comparative effect of amphotericin b on rhizoctonia sp., paecilomyces lilacinus & fusarium solani in vitro & in experimental keratitis. | 1981 | 6268532 | |
| [antifungal and antimicrobial activity of beta-ionone and vitamin a derivatives]. | the antifungal and antimicrobic activity of some derivatives of beta-ionon and vitamin a was studied. these compounds (citral, pseudo-ionon. beta-ionon aldehyde c14, ketone c18 and its derivatives--4,18-diketone, alcohol c18, semicarbazide ketone c18), as well as vitamin a and its derivatives--retinal, acetate, retinoic acid--differ in composition, structure and substituents of c-atoms in beta-ionon ring and in polyenoic chain. fusarium solani, botrytis cenerea and verticillum dahliae ii, race 4 ... | 1983 | 6364128 |
| recurrent fusarium keratomycosis: a light and electron microscopic study. | corneal buttons infected with fusarium solani, were removed during penetrating keratoplasty, and studied by light and electron microscopy. histologically, it appeared that the hyphae penetrated descement 's membrane and the posterior stroma from the anterior chamber. their cell wall, plasmalemma, and cytoplasm were preserved indicating that the fungi might be still active despite antifungal therapy, which may not be effective once the fungus penetrates descemet's membrane. the other interesting ... | 1984 | 6372614 |
| degradation of humic acids, extracted from manure and soil by some streptomycetes and fungi. | humate-decomposing microorganisms were isolated from alluvial soil, farmyard manure and peat by direct plating and enrichment techniques. peat was found to harbour more of these organisms than soil and manure. out of 21 actinomycetes and 6 fungi 9 and 3 isolates, respectively, were found to utilize manure-humate as a sole source of carbon and to a less extent as a source of both carbon and nitrogen in liquid medium. these efficient cultures were identified as streptomyces spec. (a 11 and a 12). ... | 1980 | 6448526 |
| anterior segment resection because of fusarium solani keratitis and endophthalmitis. | 1984 | 6610405 | |
| relative efficacy of miconazole against rhizoctonia sp., paecilomyces lilacinus and fusarium solani. | 1983 | 6618536 | |
| studies on the antigenic composition of aqueous cotton dust extracts. | an immune response to cotton dust components has been proposed as a possible mechanism of byssinosis. to evaluate this possibility, specific precipitating antibodies against aqueous cotton dust extracts (acde) were raised in rabbits, and a reference pattern for crossed immunoelectrophoresis (cie) was established using this extract. a significant portion of the immune response was directed against alternaria tenuis, aspergillus niger, and fusarium solani, all documented contaminants of cotton dus ... | 1983 | 6642705 |
| formation of trichothecenes by fusarium solani var. coeruleum and fusarium sambucinum in potatoes. | fusarium solani var. coeruleum can form deoxynivalenol in potato tubers and in liquid medium, although concentrations observed in the rot were highly variable; acetyldeoxynivalenol and ht-2 toxin were detected in 1 to 3 tubers only (of 57). trichothecenes were also detected in a very few (3 of 20) cultures of fusarium sambucinum in potato tubers. | 1984 | 6742832 |
| mechanism of action of cutinase: chemical modification of the catalytic triad characteristic for serine hydrolases. | cutinase from fusarium solani f. sp. pisi was inhibited by diisopropyl fluorophosphate and phenylboronic acid, indicating the involvement of an active serine residue in enzyme catalysis. quantitation of the number of phosphorylated serines showed that modification of one residue resulted in complete loss of enzyme activity. one essential histidine residue was modified with diethyl pyrocarbonate. this residue was buried in native cutinase and became accessible to chemical modification only after ... | 1982 | 6809046 |
| septic arthritis due to fusarium solani. | a case of fusarium arthritis is reported. fusarium, a mold ubiquitous in soil and on plants commonly causes keratomycosis and infects burns. recent reports demonstrate that fusarium may produce serious visceral infection in compromised hosts. disseminated infection has been universally fatal. in contrast, our case and several others with localized visceral infection were cured by intravenous amphotericin b and drainage. although fusarium grows readily on mycologic media, blood and other cultures ... | 1983 | 6842474 |
| ovicidal fungi in soils of cuba. | thirty-six strains of ovicidal fungi were isolated from 122 investigated soil samples collected in cuba. the isolated strains belong to 8 genera, 2 strains consist of sterile mycelium only. most of the strains belong to the genus fusarium (4 species, 1 undetermined strain), the remaining ones to mortierella (3 species and 6 undetermined strains), humicola (2 species), paecilomyces (2 species) and penicillium (2 species). the genera verticillium, gliocladium and cunninghamella were represented by ... | 1982 | 6890012 |
| studies on the etiology and symptomatology of root and storage rot disease of cocoyam in nigeria. | the losses caused by root and storage rot of cocoyam in nigeria are estimated as 40 to 45%. field symptoms of the disease include inhibited growth, leaf chlorosis followed by necrosis and shrivelling of affected parts, and finally premature death of the aerial portions of the plant. a large proportion of the roots are destroyed. poor production of cormels and reduced corm size are other field symptoms of the disease, differing according to the type of causal agent. botryodiplodia theobromae, fus ... | 1982 | 6891209 |
| fusarium solani endophthalmitis. | 1980 | 6966137 | |
| [perforatory keratoplasty for keratitis due to fusarium solani]. | 1981 | 7024056 | |
| the immunosuppressive effects of trichothecenes and cyclochlorotine on the antibody responses in guinea pigs. | immunosuppressive effects of trichothecenes of fusarium solani and fusarium nivale, t-2 toxin and fusarenon-x, and also of a mycotoxin of penicillium islandicum, cyclochlorotine, were studied by measuring the anti-2,4-dinitrophenyl (dnp) antibody responses in guinea pigs immunized with dnp-bovine serum albumin. among these mycotoxins, t-2 toxin alone suppressed strongly the anti-dnp antibody responses at a certain sublethal dose. with other mycotoxins, no effect was observed at any sublethal dos ... | 1982 | 7120038 |
| fusarium solani keratitis. | 1982 | 7169026 | |
| interference in the gas chromatographic determination of deoxynivalenol in cultures of fusarium solani on corn. | 1982 | 7182412 | |
| ecological distribution of fusarium solani and its opportunistic action related to mycotic keratitis in cali, colombia. | corneal ulcera in patients treated at the university hospital cali, colombia have been attributed to the fungus fusarium solani, which was isolated from patients' eyes by deep scraping. the fungus, which was characterized by culture and morphology, was found to grow well at 37 degrees c in sabouraud and potato dextrose agars and in liquid asparagine medium, in which it produced very few spores; at 40 degrees c, it survived for 3 weeks. different levels of pathogenicity were shown by the fungus w ... | 1980 | 7217337 |
| metabolites of fusarium solani related to dihydrofusarubin. | 1980 | 7251476 | |
| mechanics of chromosome separation during mitosis in fusarium (fungi imperfecti): new evidence from ultrastructural and laser microbeam experiments. | the anaphase-telophase spindle usually elongates, and it has been assumed that the spindle pushes the incipient daughter nuclei apart. to test this assumption, we used a laser microbeam to sever the central spindle of the fungus, fusarium solani, and measured the rate of separation of incipient daughter nuclei. when the microbeam was aimed beside the spindle separation occurred at a rate (8.6 micrometer/min) that did not differ significantly from the rate (7.6 micrometer/m) in unirradiated cells ... | 1981 | 7309791 |
| fusarium solani keratitis. first report from malaysia. | 1981 | 7343825 | |
| structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the n terminus. | cutinase i and cutinase ii, two extracellular enzymes produced by fusarium solani pisi, were shown to be glycoproteins containing 4.3% and 5.1% carbohydrates, respectively. upon treatment with alkali both enzymes generated chromophores which absorbed at 241 nm. treatment of both proteins with alkaline nab3h4 gave labeled protein and labeled monosaccharides. hydrolysis of the labeled protein followed by chromatographic and enzymatic analyses of the products showed that alanine, 2-aminobutyrate, p ... | 1980 | 7398618 |
| [levanase from fusarium solani-68]. | extracellular levanase was isolated from the culture of fusarium solani-68 by ammonium sulfate precipitation at 0.8 saturation and purified in part by sephadex g-150 gel chromatography. levanase showed maximum activity at ph = 6.0 and t = 45 degrees c. the levanase reaction (hydrolysis of high molecular levane with a molecular weight of 1--5 min) reached a maximum rate at a polysaccharide concentration of 6.7 mg/ml, michaelis constant being 1.5 x 10(-6) m. levanase remained stable at ph 5.4--7.5 ... | 1980 | 7433428 |
| fusarium solani infection of a hypertensive leg ulcer in a diabetic. | 1980 | 7464854 | |
| a case of mycotic keratitis caused by fusarium solani. | a 36-year-old black man, without history of systemic disease or ocular trauma developed a corneal infection in his left eye. he was treated with antibacterial antibiotic and corticosteroids for one month prior to diagnosis. fungal hyphae and chlamydospores were found in a koh preparation of the corneal scrapings, and positive cultures for fusarium solani were obtained in sabouraud dextrose agar. it is emphasized the cautious use of antibiotics and steroids in corneal diseases, and the need of co ... | 1995 | 7481475 |
| cloning of a new pectate lyase gene pelc from fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of the gene product expressed in pichia pastoris. | antibodies prepared against a pectate lyase (pla) produced by a phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating type vi) were previously found to protect the host against infection. the cdna and gene (pela) for pla were cloned and sequenced. a new pectate lyase gene, pelc, was isolated from a genomic library of f. solani pisi with pela cdna as a probe. a 1.3-kb dna fragment containing the pelc gene and its flanking regions was identified and sequenced. the coding ... | 1995 | 7487098 |
| phosphonate analogues of triacylglycerols are potent inhibitors of lipase. | 1,2-dioctylcarbamoylglycero-3-o-p-nitrophenyl alkylphosphonates, with alkyl being methyl or octyl, were synthesised and tested as irreversible inhibitors of cutinase from fusarium solani pisi and staphylococcus hyicus lipase. rapid inactivation of these enzymes occurred with a concomitant release of one mole of p-nitrophenol per mole of enzyme. with both lipases a higher reactivity was observed when the alkyl substituent on the phosphonate is a methyl rather than an octyl chain. both lipases are ... | 1995 | 7492616 |
| pathogenic effects of fusarium sulphureum, fusarium solani var. coeruleum and dry rot affected potatoes on the internal organs of rats. | rats of the wistar race were used in toxicological experiments involving fusarium sulphureum schl., f. solani var. coeruleum (sacc.) booth and potatoes damaged by these fungi. the in vivo and postmortem studies revealed that both fungi and effected tubers had hepatotoxic and nephrotoxic effects on the animals. morphological changes in the internal organs were mainly manifested by disturbances in blood circulation and regressive metamorphosis. these changes intensified proportionally to the dose ... | 1993 | 7504875 |
| triglyceride hydrolysis and stability of a recombinant cutinase from fusarium solani in aot-iso-octane reversed micelles. | a recombinant cutinase from fusarium solani was encapsulated in aot reversed micelles. physicochemical parameters of the system were optimized relative to triolein hydrolysis. kinetic studies of triglyceride hydrolysis showed a decrease in specificity with increase of the acyl chain length. stability of cutinase in the system under study is lower than in aqueous solution and decreases with increase in the water content in the system (w0 = [h2o]/[aot]). the products of triolein hydrolysis had lit ... | 1995 | 7535515 |
| phylogenetic relationships of the soybean sudden death syndrome pathogen fusarium solani f. sp. phaseoli inferred from rdna sequence data and pcr primers for its identification. | phylogenetic relationships of several species within the fusarium solani-complex were investigated using characters from the nuclear ribosomal dna. genetic variation within 24 isolates, including 5 soybean sudden death syndrome (sds) strains, was assessed using rdna sequence data and restriction fragment length polymorphic markers. by these techniques, the causal agent of soybean sds was identified as f. solani f. sp. phaseoli. in separate cladistic analyses, plectosphaerella cucumerina and nect ... | 1995 | 7579615 |
| effect of increasing inoculum sizes of pathogenic filamentous fungi on mics of antifungal agents by broth microdilution method. | inoculum size is a critical variable in development of methods for antifungal susceptibility testing for filamentous fungi. in order to investigate the influence of different inoculum sizes on mics of amphotericin b, 5-fluorocytosine, itraconazole, and miconazole, 32 clinical isolates (8 aspergillus fumigatus, 8 aspergillus flavus, 5 rhizopus arrhizus, 8 pseudallescheria boydii, and 3 fusarium solani isolates) were studied by the broth microdilution method. four inoculum sizes were studied: 1 x ... | 1995 | 7615745 |
| inversion of lipase stereospecificity for fluorogenic alkyldiacyl glycerols. effect of substrate solubilization. | we synthesized enantiomeric 1-o-alkyl-2,3-diacyl-sn-glycerol and 3-o-alkyl-1,2-diacyl-sn-glycerol containing pyrene as a fluorescent reporter and the trinitrophenylamino residue as a fluorescence quencher; both reporter groups were covalently bound to the omega end of the acyl chains at positions sn-2 and sn-3(1), respectively. the fluorescence of the intact substrate molecules was very low. chemical or enzymic release of the fatty acyl chains lead to fluorescence dequenching. the rate of lipoly ... | 1995 | 7628484 |
| construction and heterologous expression of a synthetic copy of the cutinase cdna from fusarium solani pisi. | a copy of the cutinase cdna from fusarium solani pisi was constructed starting from synthetic oligonucleotides. for this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in saccharomyces cerevisiae and aspergillus awamori. | 1995 | 7632392 |
| hyalohyphomycoses due to fusarium spp.--two case reports and review of the literature. | we report a case of a severe fusarium solani keratitis in a 82-year-old patient with a history of surgical trauma. antimycotic therapy and keratoplasty led to markedly improved vision. identification of the fungus was complicated by the fact that the isolate did not produce the typical macroconidia. the second case was a fatal disseminated fusarium verticillioides infection in a 69-year-old patient during neutropenia after chemotherapy of acute myelogenous leukemia. the patient developed pneumon ... | 1995 | 7637684 |
| the fusarium solani gene encoding kievitone hydratase, a secreted enzyme that catalyzes detoxification of a bean phytoalexin. | among the antimicrobial phytoalexins produced by phaseolus vulgaris (french bean) is the prenylated isoflavonoid, kievitone. the bean pathogen, fusarium solani f. sp. phaseoli, secretes a glycoenzyme, kievitone hydratase (ec 4.2.1.95), which catalyzes conversion of kievitone to a less toxic metabolite. among f. solani strains, those that are highly virulent to p. vulgaris also produce kievitone hydratase constitutively, suggesting that the enzyme is a virulence factor. based on the n-terminal am ... | 1995 | 7655061 |
| induction of a tomato anionic peroxidase gene (tap1) by wounding in transgenic tobacco and activation of tap1/gus and tap2/gus chimeric gene fusions in transgenic tobacco by wounding and pathogen attack. | the anionic peroxidase genes of tomato, tap1 and tap2, are induced by wounding in tomato fruits and by elicitor treatment in cell suspension cultures. these homologous genes code for anionic peroxidases that are postulated to cause polymerization of the phenolic residues into wall polymers in wound-healing and pathogen-infected tissues. an expression construct containing the entire tap1 gene with its 5' and 3' flanking sequences was introduced into tobacco by agrobacterium tumefaciens-mediated g ... | 1993 | 7678769 |
| construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. | a gene fusion between the saccharomyces cerevisiae actin gene promoter and the cdna of the fusarium solani f. sp. pisi pela gene has been constructed. this expression cassette has been introduced into the industrial wine yeast strain t73. the resulting recombinant strain is able to secrete active pela enzyme into the culture medium. in preliminary microvinification experiments the wine produced by this pectinolytic strain is indistinguishable from wine produced using the non-transformed strain o ... | 1995 | 7729670 |
| [fusarium solani peritonitis: infrequent complication of peritoneal dialysis]. | 1995 | 7734510 | |
| cloning and expression of cdna encoding a protein that binds a palindromic promoter element essential for induction of fungal cutinase by plant cutin. | previous studies showed that a palindromic sequence located at -159 base pairs is essential for induction of cutinase gene in fusarium solani f. sp. pisi (nectria haematococca mating type vi) by the hydroxy fatty acids from plant cutin and that a 50-kda nuclear protein binds to a promoter that contains this element. screening of a phage lambda gt11 expression library with the concatenated palindromic sequence as the probe identified a cdna encoding a palindrome-binding protein (pbp). nucleotide ... | 1995 | 7744822 |
| in vitro transcription from the nectria haematococca pda1 promoter in an homologous extract reflects in vivo pisatin-responsive regulation. | the pda1 gene of nectria haematococca mp vi (anamorph: fusarium solani) encodes pisatin demethylase. this enzyme detoxifies the isoflavanoid phytoalexin pisatin produced by the plant on which this fungus is pathogenic. expression of pisatin demethylase activity is induced in a mycelium by pretreatment with pisatin. we have developed homologous in vitro system which accurately initiates transcription from the pda1 promoter. transcription levels in vitro reflect the same pisatin-responsive stimula ... | 1994 | 7750146 |
| surface signaling in pathogenesis. | surface signaling plays a major role in fungal infection. topographical features of the plant surface and chemicals on the surface can trigger germination of fungal spores and differentiation of the germ tubes into appressoria. ethylene, the fruit-ripening hormone, triggers germination of conidia, branching of hyphae, and multiple appressoria formation in colletotrichum, thus allowing fungi to time their infection to coincide with ripening of the host. genes uniquely expressed during appressoria ... | 1995 | 7753774 |
| cutinase from fusarium solani pisi hydrolyzing triglyceride analogues. effect of acyl chain length and position in the substrate molecule on activity and enantioselectivity. | triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. the chain length at either position was varied, and both (r)- and (s)-enantiomers of each compound were synthesized. these pseudo triglycerides contain only one hydrolyzable ester bond, and they are ideally suited to studying the influence of the chain length at the 1-, 2-, and 3-position on lipase ... | 1995 | 7756270 |
| structural effect of reversed micelles of aot over a recombinant cutinase from fusarium solani pisi. a steady state fluorescence study. | 1995 | 7785884 | |
| molecular cloning and characterization of a pea chitinase gene expressed in response to wounding, fungal infection and the elicitor chitosan. | the fungicidal class i endochitinases (e.c.3.3.1.14, chitinase) are associated with the biochemical defense of plants against potential pathogens. we isolated and sequenced a genomic clone, dah53, corresponding to a class i basic endochitinase gene in pea, chi1. the predicted amino acid sequence of this chitinase contains a hydrophobic c-terminal domain similar to the vacuole targeting sequences of class i chitinases isolated from other plants. the pea genome contains one gene corresponding to t ... | 1995 | 7787175 |
| [severe keratomycosis. diagnosis and treatment]. | we reported three consecutive cases of severe fungal keratitis initially undiagnosed by corneal scrapping. corneal biopsy showed fungal elements of paecilomyces lilacinus, fusarium solani, scedosporium apiospermum. most cases of fungal keratitis could be treated successfully if adequate antifungal therapy were started early. early and accurate diagnosis is of major importance. the results of our cases confirm the superiority of corneal biopsy over corneal scrapping. corneal biopsy is a procedure ... | 1994 | 7822693 |
| disseminated fusarium solani infection. | disseminated fungal infections commonly occur in immunocompromised hosts; candida spp. are the most common. fusarium spp., soil saprophytes once considered pathogenic only in plants, have emerged as serious pathogens in neutropenic patients with malignancies. we describe two patients, one with acute myelogenous leukemia and the other with metastatic breast cancer, in whom disseminated fusarium solani infection developed. both patients had neutropenia and fever when generalized, tender, erythemat ... | 1995 | 7829738 |
| in vitro transcription and binding analysis of promoter regulation by a host-specific signal in a phytopathogenic fungus. | the pda1 promoter of the phytopathogennectria haematococca mpvi (anamorph fusarium solani) offers a model for regulation of a fungal virulence gene in response to plant host-specific signals. expression of the pda1 gene, encoding pisatin demethylase, is induced in culture by pisatin, the isoflavanoid phytoalexin of pea. this pisatin induction is suppressed by nutritional factors. we have been studying the mechanism of pisatin induction through in vitro identification of regulatory factors and re ... | 1994 | 7847884 |
| glyceride synthesis catalyzed by cutinase using the monomolecular film technique. | the monomolecular film technique previously used to study the kinetics of lipase hydrolysis was adapted to synthesizing oleoyl glycerides (monoolein, diolein, and triolein). the water subphase was replaced by glycerol, and a film of oleic acid was initially spread on the glycerol surface. in this system a recombinant cutinase from fusarium solani was able to catalyze oleoyl glyceride synthesis. more than 50% of the oleic acid film was acylated after 7 min of reaction. the surface pressure applie ... | 1995 | 7849021 |
| sterility and the disinfection potential of indian contact lens solutions. | ocular infection associated with microbial contamination of contact lens care products is a major problem in contact lens wearers. the sterility and the antimicrobial activity of contact lens care systems reflect their suitability for disinfection of contact lenses. these factors remain to be evaluated for the various newer contact lens care products manufactured in india. in this study, 35 bottles of contact lens solutions marketed by different manufacturing units in india were tested for steri ... | 1994 | 7927633 |
| the root epidermis-specific pea gene rh2 is homologous to a pathogenesis-related gene. | two-dimensional gel electrophoresis of pea root and root hair proteins revealed the existence of at least 10 proteins present at elevated levels in root hairs. one of these, named rh2, was isolated and a partial amino acid sequence was determined from two tryptic peptides. using this sequence information oligonucleotides were designed to isolate by pcr an rh2 cdna clone. in situ hybridization studies with this cdna clone showed that rh2 is not only expressed in root hairs, but also in root epide ... | 1994 | 7948884 |
| characterization of the pda1 promoter of nectria haematococca and identification of a region that binds a pisatin-responsive dna binding factor. | isolates of nectria haematococca (anamorph: fusarium solani) are able to detoxify the pea phytoalexin pisatin through expression of pisatin demethylase (pda). this enzyme is a substrate-inducible cytochrome p450 monooxyenase that is encoded by the pda gene family. in the current study, pda1, a highly inducible pda gene, was cloned and the 5' untranslated region was sequenced. the pda mrna levels were measured in pisatin-treated mycelium and found to increase by 20-fold over untreated control. ge ... | 1994 | 8012044 |
| a new class of tobacco chitinases homologous to bacterial exo-chitinases displays antifungal activity. | a novel chitinase gene of tobacco was isolated and characterized by dna sequence analysis of a genomic clone and a cdna clone. comparative sequence analysis of both clones showed an identity of 94%. the proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes i-iv and are designated as class v chitinases. comparison of the chitinase class v peptide sequence with sequences in the swiss protein databank revealed significant sequence s ... | 1994 | 8012401 |