Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| cloning of ndhk from soybean chloroplasts using antibodies raised to mitochondrial complex i. | a soybean shoot cdna expression library was screened with polyclonal antibodies raised against red beet complex i and several clones were identified. one clone, consisting of a 1 kb insert, was fully sequenced. the sequence of 1025 bp was found to contain two extended open reading frames and the proteins encoded were identified as the ndhk and ndhj products of the chloroplast genome. nuclear, mitochondrial and chloroplast dna was isolated and probed with a ndhk-specific probe. the chloroplast dn ... | 1992 | 1463827 |
| isolation of dna markers linked to a beet cyst nematode resistance locus in beta patellaris and beta procumbens. | in cultivated beet no useful level of resistance of the beet cyst nematode (bcn) heterodera schachtii schm. has been found, unlike the situation in wild species of the section procumbentes. stable introgression of resistance genes from the wild species into beta vulgaris has not been achieved, but resistant monosomic additions (2n = 18 + 1), diploids of b. vulgaris with an extra alien chromosome carrying the resistance locus, have been obtained. here we describe a new series of resistant monosom ... | 1992 | 1465114 |
| exploitation of gene(s) involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a pseudomonas strain. | tn5 mutagenesis and complementation analysis were used to clone a 6-kb genomic fragment required for biosynthesis of 2,4-diacetylphloroglucinol (phl) from fluorescent pseudomonas sp. strain f113. a recombinant plasmid, pcu203, containing this region partially complemented a phl production-negative mutant (f113g22) derived from strain f113. when sugar beet seeds were sown into an unsterilized soil, in which sugar beet was subject to damping-off by pythium ultimum, the emergence of sugar beet seed ... | 1992 | 1476431 |
| cell membranes and multilamellar vesicles: influence of ph on solvent induced damage. | pigment leakage from sheep and horse erythrocytes and from red beet tissue induced by non-polar solvents was determined as a function of ph. the results were compared to disruption of multilamellar vesicles (mlv) composed of phospholipids with equimolar cholesterol under identical conditions of solvent exposure and ph. solvent access to cholesterol was used to measure vesicle disruption. mlv were made from 1,2-dioleoyl phosphatidylethanolamine, sphingomyelin (sp) and various phosphatidylcholines ... | 1992 | 1487967 |
| [liver diseases of fattening bulls]. | the authors report on occurrence, causes and diagnostics of liver affections observed in fattening bulls in ukrainia between 1982 and 1988. for this purpose, 2747 bulls in 10 fattening plants had been controlled clinically once during the last month of their final fattening period (lasting, according to the feeding schedule, from the 4th until the 12th, or from the 6th until the 18th month of life), and 1318 of them were controlled for eventual hepatic lesions at slaughter. the authors found an ... | 1992 | 1505364 |
| asymmetric somatic cell hybridization in plants. i. the early effects of (sub)lethal doses of uv and gamma radiation on the cell physiology and dna integrity of cultured sugarbeet (beta vulgaris l.) protoplasts. | an investigation into the possible application of uv radiation as a pretreatment for the donor cells in asymmetric plant cell hybridization protocols has been carried out. a comparison was made between the effects of uv doses in the range 700-4200 j/m2 and those of 60co gamma radiation over the range 0.15-1 kgy on beta vulgaris suspension cell protoplasts. the investigation had two aspects. firstly, alterations to cell physiology (cell wall resynthesis, viability, division and colony formation) ... | 1992 | 1508155 |
| asymmetric somatic cell hybridization in plants. ii. electrophoretic analysis of radiation-induced dna damage and repair following the exposure of sugarbeet (beta vulgaris l.) protoplasts to uv and gamma rays. | as part of an investigation into whether it would be possible to use uv radiation as a suitable pretreatment of the donor cells in asymmetric hybridization experiments, the effects of this treatment on sugarbeet (beta vulgaris l.) protoplast dna have been determined and compared with those of gamma radiation. both nuclear and mitochondrial dnas have been examined. the dose ranges chosen had previously been determined to be potentially applicable for fusion experiments. pulsed field gel electroph ... | 2006 | 1508156 |
| gene rescue in plants by direct gene transfer of total genomic dna into protoplasts. | to study the possibility of gene rescue in plants by direct gene transfer we chose the arabidopsis mutant gh50 as a source of donor dna. gh50 is tolerant of chlorsulfuron, a herbicide of the sulfonylurea class. tobacco protoplasts were cotransfected with genomic dna and the plasmid php23 which confers kanamycin resistance. a high frequency of cointegration of the plasmid and the genomic dna was expected, which would allow the tagging of the plant selectable trait with the plasmid dna. after tran ... | 1992 | 1508682 |
| computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis e virus: delineation of an additional group of positive-strand rna plant and animal viruses. | computer-assisted comparison of the nonstructural polyprotein of hepatitis e virus (hev) with proteins of other positive-strand rna viruses allowed the identification of the following putative functional domains: (i) rna-dependent rna polymerase, (ii) rna helicase, (iii) methyltransferase, (iv) a domain of unknown function ("x" domain) flanking the papain-like protease domains in the polyproteins of animal positive-strand rna viruses, and (v) papain-like cysteine protease domain distantly relate ... | 1992 | 1518855 |
| matrix nadh dehydrogenases of plant mitochondria and sites of quinone reduction by complex i. | in order to distinguish the pathways involved in the oxidation of matrix nadh in plant mitochondria, the oxidation of nadh and nicotinamide hypoxanthine dinucleotide (reduced form) was investigated in submitochondrial particles prepared from beetroot (beta vulgaris l. cv. derwent globe) and soybeans (glycine max l. cv. bragg). nicotinamide-hypoxanthine-dinucleotide(reduced form)-oxidase activity was more strongly inhibited by rotenone than the nadh-oxidase activity but both of the rotenone-inhib ... | 1992 | 1521539 |
| tissue print-immunoblotting reveals an uneven distribution of beet necrotic yellow vein and beet soil-borne viruses in sugarbeets. | an uneven distribution of the coat protein antigens of beet necrotic yellow vein (bnyvv) and beet soil-borne (bsbv) viruses in tap roots of naturally infected sugarbeets and of bnyvv coat protein antigen in leaves and petioles of mechanically inoculated sugarbeet seedlings was detected by means of tissue print-immunoblotting. bnyvv antigen-containing areas in the tap roots were usually found underneath a root beard. occasionally bnyvv antigen was detected predominantly, but not exclusively in th ... | 1992 | 1524497 |
| dsrna cloning and diagnosis of beet pseudo-yellows virus by pcr and nucleic acid hybridization. | dsrna has been extracted from beet pseudo-yellows virus infected cucumber plants, purified to homogeneity, and cdna clones to it produced. the clones are of insufficient sensitivity to detect infection-specific rna in dot and northern blots of crude nucleic acid extracts. however, a knowledge of the sequence of these clones has been used to synthesize oligonucleotides that have been used for polymerase chain reaction amplification of specific sequences from both purified dsrna and from infected ... | 1992 | 1526749 |
| a symptom variant of beet curly top geminivirus produced by mutation of open reading frame c4. | two beet curly top virus (bctv) mutants have been constructed in vitro that contain g-to-t transversions either at nucleotide 2682 or at nucleotide 2802 within the overlapping open reading frames (orfs) c1 and c4. the mutations introduce termination codons in orf c4 without affecting the amino acids encoded by orf c1. when agroinoculated into nicotiana benthamiana the mutants caused stunting and yellowing of the plant and downward leaf curl but not the vein swelling and upward leaf curl symptoms ... | 1992 | 1529551 |
| cis-active sequences near the 5'-termini of beet necrotic yellow vein virus rnas 3 and 4. | rnas 3 and 4 of the multicomponent genome of beet necrotic yellow vein virus are dispensable for infection of chenopodium quinoa leaves. we have used mutagenesis of biologically active rna 3 transcripts to identify 5'-proximal sequences essential in cis for rna 3 amplification. one such element, box i, (nucleotides 283-292) was complementary to the first 10 residues (box i') following the 5'-terminal cap. a second cis-active element (box ii) was identified between nucleotides 237-244 and was com ... | 1992 | 1529554 |
| antibodies to mammalian and plant v-atpases cross react with the v-atpase of insect cation-transporting plasma membranes. | in immunobiochemical blots, polyclonal antibodies against subunits of plant and mammalian vacuolar-type atpases (v-atpases) cross-react strongly with corresponding subunits of larval manduca sexta midgut plasma membrane v-atpase. thus, rabbit antiserum against kalanchoe daigremontiana tonoplast v-atpase holoenzyme cross-reacts with the 67, 56, 40, 28 and 20 kda subunits of midgut v-atpase separated by sds-page. antisera against bovine chromaffin granule 72 and 39 kda v-atpase subunits cross-reac ... | 1992 | 1534830 |
| two proteins encoded by beet necrotic yellow vein virus rna 3 influence symptom phenotype on leaves. | rna 3 of the beet necrotic yellow vein virus (bnyvv) quadripartite rna genome is not essential for virus multiplication on leaves of tetragonia expansa but has dramatic effects on symptom expression. virus isolates containing rna 3 produce bright yellow local lesions while isolates lacking rna 3 produce much milder symptoms. using directed mutagenesis of cdna clones followed by in vitro synthesis of biologically active transcripts, a 25 kda open reading frame (orf) of rna 3 was shown to be respo ... | 1992 | 1537331 |
| a number of subgenomic dnas are produced following agroinoculation of plants with beet curly top virus. | in addition to ss and ds genomic dna, agroinoculation of nicotiana benthamiana plants with the logan strain of the geminivirus beet curly top virus (bctv) consistently resulted in de novo production of subgenomic dnas on initial passage. single-stranded and dsdna forms representing at least seven size classes (0.8 to 1.8 kb) of subgenomic dna were observed in total dna extracts from inoculated plants. extracts from infected sugar beet and tomato contained variable but usually smaller amounts of ... | 1992 | 1538189 |
| the sucrose carrier of the plant plasmalemma. iii. partial purification and reconstitution of active sucrose transport in liposomes. | the proteins from plasma membranes from sugar beet leaves were solubilized by 1% chaps and separated by size exclusion chromatography and by ion-exchange chromatography. the fractions enriched in sucrose transporter were monitored in three ways: differential labeling, elisa, and reconstitution in proteoliposomes. when the plasma membranes were differentially labeled by n-ethylamaleimide in the presence of sucrose, a major peak of differential labeling was found at 120 kda upon gel filtration. wh ... | 1992 | 1543711 |
| dna markers closely linked to nematode resistance genes in sugar beet (beta vulgaris l.) mapped using chromosome additions and translocations originating from wild beets of the procumbentes section. | genes conferring resistance to the beet cyst nematode (heterodera schachtii schm.) have been transferred to sugar beet (beta vulgaris l.) from three wild species of the procumbentes section using monosomic addition and translocation lines, because no meiotic recombination occurs between chromosomes of cultured and wild species. in the course of a project to isolate the nematode resistance genes by strategies of reverse genetics, probes were cloned from dna of a fragmented b. procumbens chromosom ... | 1992 | 1557034 |
| structural determinants in substrate recognition by proton-amino acid symports in plasma membrane vesicles isolated from sugar beet leaves. | amino acids are actively transported across the plasma membrane of plant cells by proton-coupled symports. previously, we identified four amino acid symports in isolated plasma membrane vesicles, including two porters for the neutral amino acids. here we investigated the effect of amino acid analogues on neutral amino acid transport to identify structural features that are important in molecular recognition by neutral system i (isoleucine) and neutral system ii (alanine and leucine). d-isomers o ... | 1992 | 1567208 |
| selenocysteyl-trnas recognize uga in beta vulgaris, a higher plant, and in gliocladium virens, a filamentous fungus. | selenocysteyl-trnas that decode uga were previously identified in representatives of three of the five life kingdoms which were the monera, animal and protist kingdoms. in the present study, we show that these trnas also occur in representatives of the two remaining kingdoms, plants and fungi; i.e., selenocysteyl-trnas which code for uga occur in beta vulgaris, a higher plant, and in gliocladium virens, a filamentous fungus. the fact that selenocysteyl-trnas are present in all five life kingdoms ... | 1992 | 1567433 |
| single- and double-stranded rnas associated with an isolate of beet soil-borne virus. | ethidium bromide staining of electrophoretically separated ssrnas and dsrnas as well as northern blot analyses with cdna clones suggested that the genome of the ahlum serotype of beet soil-borne virus (bsbv) consists of two major ssrna species of approximately 3.6 and 3.2 kb, respectively, and possibly a minor ssrna of approximately 6.0 kb. a few of our clones hybridized with both the 3.6-kb and the 3.2-kb rnas, the majority of the clones, however, hybridized only with the 3.2-kb rna. the 3.2-kb ... | 1992 | 1568837 |
| a comparison, using dsrna analysis, between beet soil-borne virus and some other tubular viruses isolated from sugar beet. | double-stranded rna preparations from chenopodium quinoa leaves inoculated with two english isolates of beet soil-borne virus (bsbv), bsbv-n and bsbv-452n, a french isolate of beet necrotic yellow vein virus (bnyvv), a swedish isolate of a tubular beet virus (86-109) or a belgian isolate of a similar virus (1530) were compared following separation on non-denaturing polyacrylamide gels. the dsrnas of bnyvv differed in mobility from those isolated from tissue infected with the other four tubular b ... | 1992 | 1588330 |
| assessment of concentrations of beet necrotic yellow vein virus by enzyme-linked immunosorbent assay. | a quantitative screening method based on enzyme-linked immunosorbent assay (elisa) was developed for beet necrotic yellow vein virus (bnyvv). with respect to the assessment of the dose-response curve, the response-error relation, and the precision profile the choice of concentrations and replications per concentration was investigated. special attention was given to control and fulfillment of validity assumptions as formulated by finney (1978). to this end the influence of the dilution medium fo ... | 1992 | 1597507 |
| occupational asthma in a beet sugar processing plant. | a patient with occupational asthma in the beet sugar processing industry is described. symptomatology, skin testing, immunologic testing, and specific bronchoprovocation testing indicate exposure to moldy sugar beet pulp was the cause of the patient's occupational asthma. cooperation between the treating physician and public health authorities is encouraged. | 1992 | 1600801 |
| efficient cell-to-cell movement of beet necrotic yellow vein virus requires 3' proximal genes located on rna 2. | rna 2 of beet necrotic yellow vein virus (bnyvv) carries six open reading frames. the four 3' proximal frames encode the proteins p42, p13, p15, and p14. the first three species present homologies to proteins encoded by three overlapping open reading frames (the triple gene block) in potexviruses, carlaviruses, and barley stripe mosaic virus. p14 does not display homology with other known plant viral proteins. the functions of p42, p13, p15, and p14 were investigated by site-directed mutagenesis ... | 1992 | 1604825 |
| single cell protein production from beet pulp by mixed culture. | different mixed cultures of trichoderma reesei and a yeast were cultivated on beet pulp (bp)-containing medium. t. reesei and kluyveromyces marxianus offered a combination that gave high scp yields (51%) and efficiently converted bp into proteins (39.4%). the yeast extract in the basal medium could be substituted and the bp level multiplicated from 2 to 4% upon using mixed culture of t. reesei and k. marxianus. under these conditions the protein yields reached to a maximum value of 54% and highe ... | 1992 | 1605920 |
| studies on oxalate oxidase from beet stems upon immobilization on concanavalin a. | oxalate oxidase (ec 1.2.3.4) was purified from beet stems and immobilized on concanavalin a. the bound enzyme showed a high resistance of denaturation and increased the storage stability at 4 degrees c. the immobilized oxidase showed a broad optimum at ph 3.5-5, compared to the free enzyme with a sharp optimum at ph 4.5. there was a 3-fold increase in the apparent km value on immobilization. the lectin interaction also eliminated the inhibitory effect produced on the enzyme by azide, nitrate and ... | 1992 | 1616490 |
| hsp70-related 65 kda protein of beet yellows closterovirus is a microtubule-binding protein. | beet yellows virus (byv) genome encodes a 65 kda protein homologous to the hsp70 family of cellular heat-shock proteins (agranovsky, a.a., boyko, v.p., karasev, a.v., koonin, e.v. and dolja, v.v. (1991) j. mol. biol. 217, 603-610). the respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). this product was found to bind to the purified microtubules with a binding constant of 4 x 10(-7) m. the binding of p65 was stimulated if atp presented in the translat ... | 1992 | 1618294 |
| in vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus rna 2: evidence that a domain of the 75-kda readthrough protein is important for efficient virus assembly. | rna 2 of the multipartite genome of beet necrotic yellow vein virus carries the cistron for 21-kda viral coat protein at its 5' extremity. the amber termination codon of the coat protein cistron undergoes suppression approximately 10% of the time so that translation continues into an adjacent 54-kda open reading frame, yielding a 75-kda readthrough protein. the roles of coat protein and the readthrough protein in infection were investigated with biologically active transcripts of rna 2. much of ... | 1992 | 1631051 |
| characterization of beet curly top virus subgenomic dna localizes sequences required for replication. | subgenomic viral dna is accumulated in nicotiana benthamiana and beta vulgaris plants agroinoculated with the geminivirus beet curly top virus. the subgenomic dna is more abundant in n. benthamiana and is distributed between two broad size groups in this host. six unique examples, ranging in size from 887 to 1311 nucleotides, have been cloned from viral double-stranded dna purified from n. benthamiana and analyzed by sequence determination. deletions are distributed throughout most of the genome ... | 1992 | 1641993 |
| effect on lipid metabolism of beet fibre in desert nomads with low habitual fibre intake. | the present study was conducted to test the efficacy of fibre supplementation for hypercholesterolaemia in subjects from the desert nomads. ten males (53 +/- 8 years of age) participated in this study which consisted of three phases: baseline-1 period (2 weeks) in which subjects were on their normal, habitual dietary intake, followed by a period of fibre supplementation (5 weeks) in which subjects were supplemented with 26 g fibre/d, and baseline-2 period (4 weeks) in which fibre supplement was ... | 1991 | 1646710 |
| apolipoprotein b gene expression in rat intestine. the effect of dietary fiber. | the effect of the dietary fiber on apo b mrna level was studied in the intestine of rats that were fed either fiber-free or high-fiber (30% sugar-beet fiber) low-fat diets for 3 weeks. the fiber diet studied does not affect jejunal apo b mrna levels but decreases the level of ileal apo b mrna. in the rat cecum, in both fiber-free and fiber groups, we failed to detect the apo b mrna. the test fiber diet feeding markedly increased fecal bile salt and cholesterol excretions. we suggest that dietary ... | 1991 | 1647973 |
| effect of type of fiber on the rate of passage and on the contribution of soft feces to nutrient intake of finishing rabbits. | one hundred thirty new zealand white x california rabbits were used to study the influence of the source of dietary fiber on several digestive criteria. five diets were formulated to provide 10% crude fiber (cf) on a dm basis. sixty percent of this dietary fiber was supplied by alfalfa hay, citrus pulp, beet pulp, grape marc, or rice hulls in the diets. weights of cecal contents, cecal ammonia concentration, and molar proportion of acetic acid in diets including pulps were higher and cecal level ... | 1991 | 1649153 |
| genomic organization and sequence analysis of the cytochrome oxidase subunit ii gene from normal and male-sterile mitochondria in sugar beet. | we have cloned and sequenced the cytochrome oxidase subunit ii (coxii) gene from both normal and cytoplasmic male-sterile (cms) sugar beet. the normal coxii (designated ncoxii) locus was found to be located 1491 bp upstream from the gene for cytochrome oxidase subunit i (coxi) on the same dna strand and to have a 1463 bp intron which split the coding sequence into two exons (382 and 398 bp). the coxii protein contains 260 amino acid residues. we have also found two copies of the coxii gene (scox ... | 1991 | 1651175 |
| the effects of sugar-beet fibre and wheat bran on iron and zinc absorption in rats. | 1991 | 1652283 | |
| the cytochrome oxidase ii gene in mitochondria of the sugar-beet beta vulgaris l. | we have cloned and analyzed the sugar-beet mitochondrial gene for cytochrome oxidase subunit ii (coxii). the sugar-beet and its deduced amino acid sequence were compared to its homologous coxii gene sequences from both monocot and dicot plants. it was found to be highly conserved (89-95%) compared to homologue in other plant species. the 780 bp coding sequence of the sugar beet coxii gene is interrupted at position 383 by a 1463 bp intron. this intron contains an additional 107 bp sequence that ... | 1991 | 1653062 |
| the influence of timing and the addition of urea to supplements containing dl-methionine on ruminal fermentation and cow weight change in beef cows. | a study was conducted to determine an optimal time for supplementation of dl-methionine in relation to time of forage intake by mature british breed-type crossbred cows, and two other experiments were conducted to determine whether ruminal ammonia concentration limited changes in disappearance rates in situ and ruminal functions caused by supplements containing dl-methionine. experiments 1 and 2 used 4 x 4 latin square designs with four cows in each experiment. treatments in exp. 1 were no suppl ... | 1991 | 1661284 |
| identification of a 170-kda protein associated with the vacuolar na+/h+ antiport of beta vulgaris. | the effect of the addition of amiloride to the growth medium was tested on the na+/h+ antiport activity of tonoplast vesicles isolated from sugar beet (beta vulgaris l.) cell suspensions. cells grown in the presence of nacl and amiloride displayed an increased antiport activity. analysis of the kinetic data showed that while the affinity of the antiport for na+ ions did not change, the maximal velocity of the na+/h+ exchange increased markedly. these results suggest the addition of more antiport ... | 1991 | 1662387 |
| effects of dietary protein-energy interrelationships on holstein steer performance and ruminal bacterial fermentation in continuous culture. | in vivo and in vitro 3 x 2 factorial experiments were conducted concurrently to evaluate the incorporation of 0, 15, or 30% sugar beet pulp (sbp) as an energy source in diets fed to growing holstein steers with either soybean meal (sbm) or alcohol-treated, defatted soybean flakes (atsbf) as primary supplemental protein sources. three groups of 42 holstein steers each were fed six different diets from 54 kg initial bw to 320 kg in three experimental periods. there were no overall sbp level x prot ... | 1991 | 1667012 |
| use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses. | a general diagnostic assay for a number of distinct luteoviruses was developed using the polymerase chain reaction (pcr) and restriction enzyme analysis. two minimally degenerate, group-specific primers were derived from previously published rna sequences of three luteoviruses. this primer pair generated specific pcr fragments of about 530 bp from extracts of plants infected with potato leafroll virus, beet western yellows virus, or new york barley yellow dwarf virus (bydv) serotypes mav, pav, r ... | 1991 | 1675249 |
| a functional arginine residue in the vacuolar h(+)-atpase of higher plants. | the arginine-specific reagent phenylglyoxal inactivated the vacuolar h(+)-atpase of red beet. inactivation by phenylglyoxal followed pseudo-first-order kinetics and a double log plot of the t1/2 of inactivation versus phenylglyoxal concentration yielded a slope of 1.18. neither inorganic anions nor dids protected from phenylglyoxal-mediated inactivation of the h(+)-atpase. indeed, cl- stimulated the rate of phenylglyoxal-mediated h(+)-atpase inactivation relative to so4(2-). atp, but not mgatp o ... | 1990 | 1690574 |
| structure and expression of tomato mitochondrial genes coding for trna(cys) (gca), trna(asn) (guu) and trna(tyr) (gua): a native trna(cys) gene is present in dicot plants but absent in monocot plants. | the nucleotide sequences of trna(asn) (guu) and trna(tyr) (gua) genes from tomato mitochondria and their flanking regions have been determined. the tomato mitochondrial trna(asn) gene is located 2.1 kb downstream from the trna(cys) gene reported previously (izuchi and sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. the trna(tyr) gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a "native" mitochondrial trn ... | 1990 | 1701109 |
| a characterization of epitopes on potato leafroll virus coat protein. | a panel of ten stable hybridoma cell lines secreting monoclonal antibodies (mabs) specific for potato leafroll virus (plrv) antigen, was produced in two fusion experiments with murine splenic and myeloma cells. using different elisa procedures and western blotting it was shown that one mab detected a continuous epitope and nine mabs reacted with conformation-dependent ones. the conformation-dependent epitopes could be separated into two groups after alkaline treatment of the virions. the mabs we ... | 1990 | 1701986 |
| influence of elisa conditions on detection of serological relationships among luteoviruses. | three variations in the elisa procedure were used in an attempt to understand the basis for serological relationships among three isolates of barley yellow dwarf virus (bydv-rpv-i from illinois, bydv-rpv-n from new york and bydv-pav from illinois), beet western yellows virus (bwyv) and soybean dwarf virus (sdv). detection of serological relationships was dependent on the state of the virus particle (e.g. dissociated or intact) and the method of detection (e.g. direct or indirect). in indirect el ... | 1991 | 1713915 |
| biologically active transcripts of a large satellite rna from arabis mosaic nepovirus and the importance of 5' end sequences for its replication. | synthetic transcripts of a satellite rna associated with a lilac isolate of arabis mosaic nepovirus (armv) were made from cdna clones. transcripts having either six (m1r) or 29 (m3r) extra nucleotides at their 5' ends replicated in the presence of armv genomic rna in manually inoculated chenopodium quinoa plants, even though m1r also differs from the native sequence at nucleotide position 2. transcript 12r, which has 11 guanosyl residues and 27 other nucleotides not present in the natural satell ... | 1991 | 1722500 |
| changes in development and ultrastructure of aspergillus niger mycelium, strain with increased tolerance to toxic substances from beet molasses. | effects of a defoamer and toxic molasses compounds on development and ultrastructure of a. niger mycelium, strain z, characterized by high tolerance to these substances and producing citric acid in surface fermentation on proper molasses media with 70% yield were presented. spumol bj in concentration of 5 microliters/100 cm3 as well as toxic molasses compounds stimulated the process of swelling and germinating of conidia. moreover, giant conidia, unable to germinate, appeared. developing myceliu ... | 1991 | 1726624 |
| synthesis of full-length transcripts of beet western yellows virus rna: messenger properties and biological activity in protoplasts. | full-length cdna of beet western yellows virus genomic rna has been cloned behind the bacteriophage t7 rna polymerase promoter of the transcription vector bs(-). the in vitro run-off transcription product obtained in the presence of t7 rna polymerase and m7gpppg cap has the same messenger properties as natural viral rna in in vitro translation systems. the full-length transcript was also able to infect chenopodium quinoa protoplasts inoculated by electroporation. infection could be followed by t ... | 1992 | 1727597 |
| salt-inducible betaine aldehyde dehydrogenase from sugar beet: cdna cloning and expression. | members of the chenopodiaceae, such as sugar beet and spinach, accumulate glycine betaine in response to salinity or drought stress. the last enzyme in the glycine betaine biosynthetic pathway is betaine aldehyde dehydrogenase (badh). in sugar beet the activity of badh was found to increase two- to four-fold in both leaves and roots as the nacl level in the irrigation solution was raised from 0 to 500 mm. this increase in badh activity was paralleled by an increase in level of translatable badh ... | 1992 | 1731961 |
| health education in school children. | a researcher compared responses to a pretest questionnaire with those a posttest questionnaire completed by 304 12-16 year old school children in india to assess health knowledge of school children. the children attended either a public school or a private school in bombay or a rural school in kazli operated by a charitable institution. the researcher administered the posttest 4 weeks after health education talks and demonstrations. before the health education course, children at all 3 schools h ... | 1991 | 1752682 |
| nucleotide sequence of cdna encoding the coat protein of beet yellows virus. | a cdna clone of beet yellows viral rna expressed the viral coat protein gene in e. coli. the sequence of the 2724 nucleotide insert revealed three open reading frames, the 3' of which was shown to be the coat protein cistron. this cistron is expressed in e. coli, in spite of there being no obvious ribosome binding site upstream. | 1991 | 1771765 |
| factors affecting the production of saccharomyces cerevisiae from sugar beet pulp. | factors affecting the production of s. cerevisiae from sugar beet pulp after acid hydrolysis were investigated. maximum yield and economic coefficient were obtained at sugar concentration of 7.15% after an incubation period of 72 h at 30 degrees c and ph 6.0 using a mixture (1:1) of ammonium sulphate and ammonium nitrate (0.2 g/g sugar) as nitrogen source. hplc analysis of beet pulp hydrolyzate showed that hexoses especially glucose serve as the most suitable sugars with regard to s. cerevisiae ... | 1991 | 1787848 |
| a nondestructive method for peptide bond conjugation of antigenic haptens to a diphtheria toxoid carrier, exemplified by two antisera specific to acetolactate synthase. | a method for the preparation of an activated protein carrier is described: protein carboxyl groups are transformed into n-hydroxysulfosuccinimide esters, a structure that will react with primary amino groups under amide bond formation. although the activated ester is unstable under aqueous conditions, a significant amount of hapten molecules can be bound covalently to the carrier under very mild conditions. ligands can be peptides or other molecules possessing a primary amino group. the method a ... | 1991 | 1799217 |
| [effect of emissions from the glass factory "sudety" in szczytna on the levels of lead and fluorine in selected vegetables and fruit collected in 1989]. | the objective of these studies was to examine the lead and flurorine contents in vegetables and apples exposed to emission of these elements by the "sudety" glassworks in szczytna (wałbrzych voivodeship). after mineralization of plant material, lead was determined by atomic absorption spectrometry (aas). fluorine was isolated from plant material samples by microdiffusion and was assayed spectrophotometrically using its complex with alizarin. the results indicate that 68% of the vegetables displa ... | 1991 | 1803439 |
| sensitivity and specificity of nucleic acid probes for potato leafroll luteovirus detection. | complementary dna (cdna) probes, prepared by nick-translation or by oligolabelling of a 520 bp fragment representing residues 4741 to 5261 in the potato leafroll luteovirus (plrv) sequence, were equally sensitive, with a detection limit equivalent to sap from about 600 micrograms of infected potato or nicotiana clevelandii leaf tissue and to rna from about 120 micrograms tissue. increasing the concentration of oligolabelled probe gave similar results with shorter autoradiographic exposures, but ... | 1991 | 1804851 |
| induction, purification and characterisation of arabinases produced by aspergillus niger. | the induction of arabinases in aspergillus niger n400 was studied on different simple and complex carbon sources. sugar beet pulp was found to be an inducer of three arabinan degrading enzymes (alpha-l-arabinofuranosidase a, alpha-l-arabinofuranosidase b and endoarabinase). these enzymes were purified from a. niger culture fluid after growth of the fungus in medium employing sugar beet pulp as the carbon source and were characterised both physico-chemically (mw 83,000, 67,000, 43,000 da and, pi ... | 1991 | 1814275 |
| the detection of beet western yellows virus and beet mild yellowing virus in crop plants using the polymerase chain reaction. | oligonucleotide primers were synthesised corresponding to conserved sequences between three isolates of beet western yellows virus (bwyv), flanking a 913 base fragment of bwyv genomic rna. using the polymerase chain reaction (pcr), these primers successfully amplified the target fragment in total rna extracts from two oilseed rape plants infected with different isolates of bwyv. the pcr products were readily detected by staining with ethidium bromide following agarose gel electrophoresis, but th ... | 1991 | 1816257 |
| application of scale-down experiments in the study of citric acid biosynthesis. | problems associated with the development of submersed citric acid biosynthesis on sugar beet molasses for process improvement were solved by scale-down experiments: the determination of optimal temperature profile, optimal ph value, influence of otr and inoculum quality. the optimal ph value at the start of the process was 6.4 with 3% of inoculum volume and temperature profile between 28-30 degrees c. in the case of otr which is scale-dependent, we prefer to use doc pilot plant data for the tran ... | 1991 | 1821875 |
| nucleotide sequence analysis and genomic organization of the ny-rpv isolate of barley yellow dwarf virus. | cdna clones representing the ssrna genome of the ny-rpv isolate of barley yellow dwarf luteovirus (bydv) were sequenced and 5600 nucleotides of the genome were determined. the deduced genome organization has limited similarity to that of another bydv isolate, vic-pav, but is identical to that of beet western yellows (bwyv) and potato leafroll (plrv) luteoviruses. ny-rpv has six major positive-sense open reading frames (orfs) and, by comparison with rna-dependent rna polymerase and nucleic acid h ... | 1991 | 1840612 |
| vacuolar proton-pumping pyrophosphatase in beta vulgaris shows vectorial activation by potassium. | previous work with membrane vesicles has demonstrated an absolute dependence on k+ for proton translocation by the inorganic pyrophosphatase (h(+)-ppase: ec 3.6.1.1) from the vacuolar membrane (tonoplast) of higher plants. using intact vacuoles from sugar beet (beta vulgaris) storage tissue, we have monitored pp1-dependent currents by patch clamp in 'whole vacuole' mode. serial k+ substitutions were made at both tonoplast faces. the results show that k+ activation occurs only at the cytosolic fa ... | 1991 | 1847114 |
| serum lipid and fecal bile acid changes with cereal, vegetable, and sugar-beet fiber feeding. | thirty-four subjects consumed six controlled formula diets for 3 wk each, supplemented with 0 g added fiber, 10 and 30 g dietary fiber as wheat bran (wb), 10 and 30 g dietary fiber as mixed vegetable fiber (vf), and 30 g dietary fiber as sugar-beet fiber (sbf). serum cholesterol changes for fiber free, 10 g wb, 30 g wb, 10 g vf, 30 g vf, and 30 g sbf (-0.13, -0.18, -0.05, -0.17, -0.24, and -0.70 mmol/l, respectively) were significant for 30 g vf and 30 g sbf. reduction in total cholesterol with ... | 1991 | 1850577 |
| metabolic effects and clinical value of beet fiber treatment in niddm patients. | in the present study a randomized cross-over design was used to determine the clinical usefulness of adding 16 g of beet fiber to the ordinary diet of non-insulin dependent diabetic (niddm) out-patients. in addition, fiber effects on the gastrointestinal hormone responses to a standardized test meal were evaluated. the study included five patients treated with diet alone and eight patients treated with diet and sulphonylurea (su). beet fiber supplementation resulted in a 10% reduction (p less th ... | 1991 | 1850691 |
| ubiquinol-cytochrome c oxidoreductase of higher plants. isolation and characterization of the bc1 complex from potato tuber mitochondria. | a procedure is described for isolation of active ubiquinol-cytochrome c oxidoreductase (bc1 complex) from potato tuber mitochondria using dodecyl maltoside extraction and ion exchange chromatography. the same procedure works well with mitochondria from red beet and sweet potato. the potato complex has at least 10 subunits resolvable by gel electrophoresis in the presence of dodecyl sulfate. the fifth subunit carries covalently bound heme. the two largest ("core") subunits either show heterogenei ... | 1991 | 1851164 |
| evidence that the 75k readthrough protein of beet necrotic yellow vein virus rna-2 is essential for transmission by the fungus polymyxa betae. | two mutant strains of beet necrotic yellow vein virus (bnyvv) containing deletion mutants of rna-2 were produced during serial passage in mechanically inoculated tetragonia expansa leaves. the mutant strains were referred to as s-0a (rna-1 + 2a) and g-0b (rna-1 + 2b). rna-2a and rna-2b were about 4.3 kb and 4.2 kb in length, respectively, whereas normal sized rna-2 was about 4.8 kb in length. in vitro translation and immunoblot analysis showed that rna-2, rna-2a and rna-2b all directed synthesis ... | 1991 | 1856688 |
| reconstitution of active sucrose transport in plant proteoliposomes. | the proteins of purified plasma membranes from sugar beet (beta vulgaris l.) leaf were solubilized and separated on a size exclusion column. the fractions eluted from the column were monitored by elisa with antibodies directed to a putative sucrose carrier protein. the peak most reactive in elisa was approximately 120 kda, and yielded a 40 kda peak after denaturation by sds. the 120-kda peak was recovered and used for reconstitution experiments with asolectin. upon imposition of an artificial ph ... | 1991 | 1864359 |
| phylogeny of capsid proteins of rod-shaped and filamentous rna plant viruses: two families with distinct patterns of sequence and probably structure conservation. | computer-assisted comparative analysis of all available amino acid sequences of the capsid proteins of positive strand rna plant viruses with helical capsids is described. two distinct families of homologous proteins were delineated through statistically significant sequence similarities, one including the capsid proteins of rod-shaped viruses (tobamo-, tobra-, hordei-, and furoviruses) and the other those of filamentous viruses (poty-, bymo-, potex-, carla-, and closteroviruses). it was conclud ... | 1991 | 1871982 |
| the nucleotide sequence and luteovirus-like nature of rna 1 of an aphid non-transmissible strain of pea enation mosaic virus. | an examination of the genomic strategy of pea enation mosaic virus (pemv) rna 1 has verified strong organizational and sequence relationships between pemv and the beet western yellows-potato leafroll luteovirus subgroup. sequence analysis of rna 1 demonstrated five predominant open reading frames (orfs). the extreme 5' orf encodes a 34k product of unknown function. the second orf encodes an 84k product which overlaps 90% of orf 1 (in a unique reading frame) and is expressed by internal initiatio ... | 1991 | 1875194 |
| influence of the substituents of the carboxyl groups and of the rhamnose content on the solution properties and flexibility of pectins. | viscometric measurements were carried out on well-characterized apple, citrus, sugar-beet pectins in order to analyse the effect of the nature and the amount of substituents (methyl, amide, acetyl groups) and of the rhamnose content on the flexibility of the polymeric backbone. through the dependence of the intrinsic viscosity with the ionic strength the flexibility parameter b was determined. b values between 0.072 and 0.017 indicate that pectins are relatively stiff molecules. however, an incr ... | 1991 | 1888715 |
| the phylogeny of rna-dependent rna polymerases of positive-strand rna viruses. | representative amino acid sequences of the rna-dependent rna polymerases of all groups of positive-strand rna viruses were aligned hierarchiacally, starting with the most closely related ones. this resulted in delineation of three large supergroups. within each of the supergroups, the sequences of segments of approximately 300 amino acid residues originating from the central and/or c-terminal portions of the polymerases could be aligned with statistically significant scores. specific consensus p ... | 1991 | 1895057 |
| effect of recombinant beet necrotic yellow vein virus with different rna compositions on mechanically inoculated sugarbeets. | beet necrotic yellow vein virus (bnyvv) inocula with different rna compositions were prepared from infectious transcripts of rnas 3 and 4 and the rg 1 isolate, which has a genome consisting only of rnas 1 and 2. the recombinant viruses were inoculated on 6- to 8-day-old sugarbeet seedlings by 'vortexing'. inocula containing rnas 1 and 2 or 1, 2 and 4 produced some growth reduction, but the most dramatic effects, with yield reductions of about 95% in a highly susceptible variety, were seen when r ... | 1991 | 1895061 |
| replicational release of geminivirus genomes from tandemly repeated copies: evidence for rolling-circle replication of a plant viral dna. | agrobacterium-mediated inoculation of nicotiana benthamiana plants with ti plasmids containing tandem genome repeats derived from different strains of the gemini-virus beet curly top virus (bctv) resulted in the production of unit-length recombinant progeny genomes in systemically infected plants. when two putative plus-strand origins of replication were present in constructs used as inocula, a replicational escape mechanism was favored that resulted in progeny genomes of a single predominant ge ... | 1991 | 1896448 |
| n,n-diethylhydroxylamine: a new electron donor to photosystem ii. | diethylhydroxylamine, when added to beet spinach thylakoid membranes in the reaction mixture enhanced both photosystem ii mediated dichlorophenolindophenol photoreduction and whole chain electron transport supported by methyl viologen. diethylhydroxylamine supports dichlorophenolindophenol photoreduction when oxygen evolving complex is inactivated by hydroxylamine washings. all the electron transport assays were found to be highly sensitive to diuron, indicating that diethylhydroxylamine donates ... | 1991 | 1898394 |
| survival of listeria monocytogenes in a food colorant derived from red beets. | three commercial lots of the red beet colorant were inoculated to contain circa 10(3) to 10(7) listeria monocytogenes strains california, v7, or scott a per milliliter and stored for 56 d at 7 degrees c. mcbride listeria agar was used to determine numbers of survivors. selected colonies thought to be l. monocytogenes were confirmed biochemically. when necessary, samples were tested by cold enrichment (up to 8 weeks). samples of colorant initially containing 10(3) to 10(4) strain california/ml we ... | 1991 | 1903135 |
| cdnas of beet necrotic yellow vein virus rnas 3 and 4 are rendered biologically active in a plasmid containing the cauliflower mosaic virus 35s promoter. | cdnas of beet necrotic yellow vein virus rnas 3 and 4 could be rendered biologically active when they were placed under the control of the cauliflower mosaic virus 35s promoter and polyadenylation signal. although the 35s in vivo transcripts should have contained up to forty 5' and several hundred 3' nonviral nucleotides, the progeny viral rnas had the same sizes as in naturally infected sugarbeets. the progeny rnas did not hybridize with the nonviral sequences indicating that they were apparent ... | 1991 | 1926790 |
| [the effect of chronic uvb-irradiation on the growth, development and productivity of fodder beet]. | in a field study in 1989, the effect of additional uv-b-irradiation (with an intensity of 0.34, 0.49 or 0.77 w/m2, daily exposure of 17.3, 26.7 or 39.1 kj/m2, and total exposure of 1457.3, 2248.8 or 3285.6 kj/m2) on the growth, development and productivity of fodder beet var. eckendorf yellow was investigated. the three levels of irradiation caused a delay of plant growth and a significant reduction of plant height (18-26%), air-dry weight of leaves (32-43%), wet weight of leaves (48-52%), and w ... | 2006 | 1960946 |
| dietary fiber for dogs: i. effects of graded levels of dietary beet pulp on nutrient intake, digestibility, metabolizable energy and digesta mean retention time. | the optimal level of beet pulp (bp) inclusion in a meat-based dog diet and the effects of graded levels of dietary bp on fecal excretion responses and mean retention time of marked fiber in the gastrointestinal tract of the dog were evaluated using 30 female english pointers assigned to isonitrogenous diets containing 0, 2.5, 5.0, 7.5, 10.0 or 12.5% bp (dm basis). beet pulp replaced portions of dietary cornstarch. digestibilities of dm and om decreased by an average of 6% when comparing diets co ... | 1990 | 1962765 |
| dietary fiber for dogs: ii. iso-total dietary fiber (tdf) additions of divergent fiber sources to dog diets and their effects on nutrient intake, digestibility, metabolizable energy and digesta mean retention time. | the objectives of this study were to examine widely divergent fiber sources for their efficacy as ingredients in a meat-based dog diet and to determine the effects of these fibers on fecal excretion responses and mean retention time of marked fiber in the gastrointestinal tract of the dog. fiber sources tested included beet pulp (bp), tomato pomace (tp), peanut hulls (ph), wheat bran (wb) and alkaline hydrogen peroxide-treated wheat straw (ahpws). diets were isonitrogenous (5.3% n) and iso-total ... | 1990 | 1962766 |
| [the effect of food fiber on the biological value of meat products]. | the effect of food fibres of wheat bran, beet pectin and methylcellulose on the biological value of meat proteins has been studied in experiments on the growing rats. it has been shown, that up to 1% on food fibres of wheat bran and up to 0.5% of pectin and methylcellulose can be added to meat products without spoiling their qualitative indices. higher levels of these substances cause the reduction of the biological value of meat products. | 1990 | 1963612 |
| the estimation of nutritive value of dairy cattle feed. | a survey is given of different methods used for the estimation of the net energy lactation and the digestible crude protein content of forages (grass hay, grass silage, maize silage) as well as tuber crops (fodder beet, turnips, raw potatoes), by-products (dried sugarbeet pulp, pressed sugarbeet pulp), compound feeds and raw ingredients for dairy cattle. the following methods are discussed: --methods only based on chemical parameters: crude fibre and cell walls. --methods using rumen fluid from ... | 1990 | 1963769 |
| [does sugar play a role in the development of gastroenterologic diseases (crohn disease, gallstones, cancer)?]. | the consumption of cane- and beet-sugars per person has today reached a level of 100 g per day. in a discussion of the role of sugar in gastrointestinal diseases there must be a differentiation between a direct effect directly related to high sugar-consumption, and an indirect effect where sugar displaces other nutritive substances in the diet. not only epidemiological, but also experimental data support that sugar has no direct, toxicological, carcinogenic or disease-inducing disposition for ga ... | 2007 | 1964756 |
| cytoplasmic male sterility in beta is associated with structural rearrangements of the mitochondrial dna and is not due to interspecific organelle transfer. | chloroplast (ct) and mitochondrial (mt) dnas from four cytoplasmic male sterile (cms) and 22 normal fertile sugar beet lines and accessions of wild beets from the genus beta have been compared with restriction analyses and southern hybridizations. we have used restriction analyses of ctdna as a phylogenetic marker to confirm the taxonomic relationships between the different cytoplasms. according to the ctdna data, all four cms cytoplasms belong to the same taxonomic section, beta. restriction pa ... | 1990 | 1979822 |
| nucleotide sequence of the 3'-terminal half of beet yellows closterovirus rna genome: unique arrangement of eight virus genes. | the sequence of 6746 nucleotides representing the 3'-proximal half of the beet yellows closterovirus (byv) genome was determined. in the direction 5' to 3', the sequence was composed of eight open reading frames (orfs) potentially encoding proteins of 6.4k (orf2), 65k (orf3), 64k (orf4), 24k (orf5), 22k (orf6), 20k (orf7) and 21k (orf8). an incomplete orf, orf1, encoded the c-terminal part of a putative rna-dependent rna polymerase, most closely related to polymerases of tricornaviruses; the put ... | 1991 | 1990061 |
| shortened forms of beet necrotic yellow vein virus rna-3 and -4: internal deletions and a subgenomic rna. | beet necrotic yellow vein virus rna-3 and rna-4, produced as full-length biologically active transcripts in vitro, can undergo spontaneous internal deletions when inoculated onto chenopodium quinoa leaves along with rna-1 and -2. the deletion process is specific, giving rise to only a few major species, and can be rapid; deleted forms appear after only one or two passages in leaves. in one of the shortened forms of rna-4, the deletion precisely eliminated one copy of a 15 nucleotide (nt) direct ... | 1991 | 1993870 |
| putative 65 kda protein of beet yellows closterovirus is a homologue of hsp70 heat shock proteins. | a portion of the rna genome of beet yellows closterovirus (byv) has been sequenced encompassing a complete long open reading frame (orf) potentially encoding a 65 kda protein. the sequence of this putative protein was strikingly similar to those of hsp70-related heat shock proteins. the counterparts of all the eight segments strongly conserved in hsp70s could be confidently identified in the byv 65 kda protein. it is suggested that some of these segments might be the atp-binding site(s) and that ... | 1991 | 2005613 |
| rapid control of purity for the cytoplasm of male-sterile seed stocks by means of a dot hybridization assay. | to produce hybrids, one member of the parental line is genetically made male-sterile. this male-sterile trait is encoded by mitochondria so that it is maternally inherited. consequently, the progeny of a male-sterile plant is fully sterile. nevertheless, during the handling of cytoplasmic male-sterile seed stocks, some mixture with seeds of the maintainer lines can occur. up to the present time, the only way to check the homogeneity of the cytoplasmic male-sterile seed stock was to grow the plan ... | 1991 | 2017200 |
| flavomycin effects on performance of beef bulls and nutrient digestibility in wethers. | for 28 weeks a feeding experiment was conducted to determine the effects of dietary flavomycin on performance of beef bulls. in the first and second half of the trial, 40 animals (average initial weight 352 kg) had free access to corn silage or beet pulp, respectively, both supplemented with an appropriate concentrate containing 0 or 10 mg/kg of the antibiotic. daily flavomycin intake was 39 to 46 mg when the animals were on the corn silage diet and 51 to 54 mg when they were fed the beet pulp d ... | 1991 | 2026115 |
| [copper poisoning of sheep after ad libitum supplementation of rations by a mineral feed]. | within 8 weeks 22 of 400 sheep died with symptoms of cu intoxication. in the liver of 2 dead sheep cu contents of 491 and 830 mg/kg dry matter were found. the concentration of copper in the concentrate and other feedstuffs (grass, hay, straw, kale, dried sugar beet pulp) could not explain the development of cu-toxicosis. the mineral-vitamin-supplement contained 86.7 mg cu/kg. obviously the cu intoxication of previously cu loaded sheep was provoked by offering the mineral supplement ad libitum (w ... | 1991 | 2044468 |
| pigment stoichiometry of a newly isolated d1-d2-cyt b559 complex from the higher plant beta vulgaris l. | two d1-d2-cyt b559 complexes with different pigment stoichiometry were isolated from the higher plant b. vulgaris. the procedures for isolating both complexes only differed in the washing time of the deae column with 50 mm tris-hcl, ph 7.2, 0.05% triton x-100 and 30 mm nacl. when the column was washed until the eluate had an absorbance of 0.01 at 670 nm, the isolated d1-d2-cyt b559 complex presented a pigment stoichiometry of 6 chlorophyll a, 2 beta-carotene, and 1 cytochrome b559 per 2 pheophyt ... | 1991 | 2044763 |
| [the energetic utilization of sugar beets, dried chips, cane sugar and apple pectin by growing swine. 2. nitrogen and energy metabolism]. | the energetic utilization of rations with raw and steamed sugar beets, dried sugar beet pulp (16 measured values each), sucrose and apple pectin (8 measured values each) as supplement feed to a basic ration was measured at 4 growing pigs each in the live weight range of between 40 and 130 kg. the utilization of the energy in the rations amounted to 69.1 +/- 5.5, 68.3 +/- 7.1, 60.9 +/- 5.8, 70.5 +/- 4.1 and 69.9 +/- 6.9% (same sequence as above). the utilization values for the supplemented feedst ... | 1991 | 2048968 |
| detection of orange juice adulteration with beet medium invert sugar using anion-exchange liquid chromatography with pulsed amperometric detection. | carbohydrate analysis of 5 beet medium invert sugar (bmis) samples and 10 pure orange juices was carried out using anion-exchange chromatography with a pulsed amperometric detector. this analysis revealed the presence of several oligosaccharides in bmis that were in either low concentration or nonexistent in the orange juice samples. these oligosaccharides may be naturally present in sugar beets or synthesized during the acid and/or enzyme catalyzed hydrolysis of sucrose during the production of ... | 2015 | 2050615 |
| cloning and characterization of a beta vulgaris satellite dna family. | three members of the bamhi-sequence family of sugar beet (beta vulgaris) have been cloned in escherichia coli and compared by sequence analysis. the sequence family shows the typical features of eukaryotic satellite dna, e.g., organization in tandem arrays and sequence divergence. a typical ladder pattern of a monomer (327 bp) and multiple oligodeoxyribonucleotides have been observed. the bamhi monomer is a + t-rich (69%) and does not show any similarity to other known plant satellite dnas. | 1991 | 2055488 |
| trophic relationships between saccharomyces cerevisiae and lactobacillus plantarum and their metabolism of glucose and citrate. | glucose and citrate are two major carbon sources in fruits or fruit juices such as orange juice. their metabolism and the microorganisms involved in their degradation were studied by inoculating with an aliquot of fermented orange juice a synthetic model medium containing glucose and citrate. at ph 3.6, their degradation led, first, to the formation of ethanol due to the activity of yeasts fermenting glucose and, eventually, to the formation of acetate resulting from the activity of lactobacilli ... | 1991 | 2059031 |
| [the use of the sugar beet in swine nutrition. 2. the effect of different preparation processes on the apparent prececal and fecal digestibility]. | with ileum cannulated sows were tested the apparent precaecal and faecal digestibility of crude nutrients from raw and thermically treated fodder sugar beets of size "rosamona". two samples from two different harvest years, differing extremely in the content of disaccharides were tested. the crude nutrient digestibility affected stronger by content of sucrose then by treatment. fodder sugar beets with contents of disaccharide lower then 400 g/kg dm showed low precaecal and faecal digestibilities ... | 1990 | 2076055 |
| [atropine poisoning by mandragora autumnalis. a report of 15 cases]. | anticholinergic syndrome (as) due to accidental poisoning is exceptional. mandragora contains a high concentration of atropine, hiosciamine and scopolamine. we have evaluated 15 patients with as due to poisoning by mandragora autumnalis, distributed in two family groups. the latency period since the ingestion was 1-4 hours (means = 2.7 +/- 0.9). the clinical features corresponded to an as of variable severity. all patients had blurred vision and dryness of mouth, nine (60%) had difficult micturi ... | 1990 | 2087109 |
| [extent and effect of fermentation in the large intestine of swine. 2. extent of fermentation, pattern of volatile fatty acids and in vitro formation rate]. | after adaptation to 8 extremely different rations 40 pigs with 108 to 125 kg lw and 6 lactating sows with 183 kg were slaughtered to obtain digesta of the hindgut. from digesta of caecum and three sections of the colon microbial metabolites and in vitro-vfa-production rates were determined. ph-values increased from 5.5 to 6.3 from caecum to the end of colon. vfa-concentrations and -production-rates decreased along the bowel to one third. highest fermentations came from rations with 50% dried bee ... | 1990 | 2090024 |
| [energetic utilization of sugar beets, dried shreds, sucrose and apple pectin by growing swine. 1. study overview and composition and digestibility of feed and rations]. | total metabolism experiments with a basic ration were carried out over two measuring periods and with a basic ration plus supplements of raw sugar beets, steamed sugar beets, dried sugar beet pulp, sucrose and apple pectin resp. with 4 pigs per test group and 4 test groups (lw 40-135 kg). live weight gain was approximately equal after the feeding of raw and steamed sugar beets. it amounted to 650 g/animal and day in the first experimental period and to 715 g in the second. after feeding dried su ... | 1990 | 2090026 |
| risk factors for clinical mastitis in herds with a low bulk milk somatic cell count. 1. data and risk factors for all cases. | the incidence rate of clinical mastitis in 125 herds with a low annual bulk milk scc (less than 150,000 cells/ml) was modeled using a poisson regression model. the rate of clinical mastitis was significantly associated with some variables that increased the exposure to environmental microorganisms: poor cubicle cleanliness increased the mastitis rate; rubber mats in cubicles were associated with a higher incidence; and drinking water from sources other than public water also increased the rate o ... | 1990 | 2099368 |
| health effects of pesticides on female beet growers. | the working conditions of female beet growers and their health effects were studied. dust, chemical substances, physical strain and the forced working posture were among the occupational factors producing adverse health effects. contact with pesticides is believed to be the major factor in the development of chronic pathology and temporary disability among female beet-growers. there is a need to promote a system of preventive measures, including comprehensive biological monitoring and control of ... | 2004 | 2100767 |
| sugarbeet mitochondria contain an open reading frame showing extensive sequence homology to the subunit 2 gene of the nadh: ubiquinone reductase complex. | sequence analysis of a transcribed region of mitochondrial dna (mtdna) from male fertile sugarbeet (beta vulgaris l.) revealed an open reading frame showing extensive sequence homology to the subunit 2 gene of the nadh: ubiquinone reductase complex (nad2). sugarbeet nad2 in common with its proposed counterpart in animal mitochondria has no intron and thereby differs from the corresponding chloroplast gene. northern rna analysis of sugarbeet nad2 suggested that transcription of this locus gives r ... | 1990 | 2115110 |
| identification of the udp-glucose-binding polypeptide of callose synthase from beta vulgaris l. by photoaffinity labeling with 5-azido-udp-glucose. | the photoaffinity probe 5-azidouridine 5'-[beta-32p]diphosphate glucose (5n3[32p]udp-glc) was used to identify a 57-kda polypeptide as a strong candidate for the udp-glc-binding polypeptide of udp-glucose: (1,3)-beta-glucan (callose) synthase from red beet (beta vulgaris l.) storage tissue. unlabeled 5n3udp-glc was a competitive inhibitor of callose synthase with a ki of 310 microm. callose synthase was purified from plasma membranes by a two-step solubilization with 3-[(3-cholamidopropyl)dimeth ... | 1990 | 2137125 |