Publications

anaplasmataceae in wild ungulates and carnivores in northern spain.	wild vertebrates are essential hosts for tick-borne diseases but data on the prevalence and diversity of anaplasma spp. in wildlife are scarce. in this study, we used real-time pcr to investigate the distribution of anaplasma species in spleen samples collected from 625 wild animals (137 cervids, 227 wild boar, and 261 carnivores) in two regions in northern spain. a first generic real-time pcr assay was used to screen for the presence of anaplasma spp. followed by a second species-specific multi ...	2016	26596894
development of an immunochromatographic strip for the serodiagnosis of theileria infection in sheep.	theileria uilenbergi and t. luwenshuni are tick-borne protozoan parasites, transmitted by haemaphysalis qinghaiensis and h. longicornis, respectively. they are the main causative agents of theileriosis in small ruminants in china. the disease has resulted in severe economic losses and hindered the development of sheep and goat husbandry industry in the endemic regions.	2015	26631226
evidence of co-infection with mycobacterium bovis and tick-borne pathogens in a naturally infected sheep flock.	ticks are responsible for the transmission of pathogens of veterinary importance, including those affecting sheep. the current study was designed to investigate co-infections with tick-borne and other pathogens in a naturally infected sheep flock with poor health condition using serology and pcr. infection with anaplasma ovis was detected by serology and pcr in 56% of the animals. the presence of rickettsia spp. of the spotted fever group (sfg) was detected by pcr and sequence analysis in 31% of ...	2016	26726806
first molecular evidence for the presence of anaplasma dna in milk from sheep and goats in china.	anaplasmosis, a disease caused by bacteria in the genus of anaplasma, imposes economic constraints on animal breeders and also threatens human health. in the present study, we investigated the presence of anaplasma spp. dna in milk collected from sheep and goats in china. a total of 120 milk samples and 414 field-sampled blood specimens from sheep and goats were analyzed by pcr and dna sequencing. anaplasma ovis was detected in 12 milk samples (three from sheep and nine from goats). the blood sp ...	2016	27038248
analysis of risk factors and prevalence of haemoplasma infection in dogs.	mycoplasma haemocanis (mhc) and 'candidatus mycoplasma haematoparvum' (cmhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. this study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from nigeria and describe any risk factors for infection. canine haemoplasma species-specific and generic haemoplasma qpcr assays were used. the species-specific qpcr assays found mhc infection in 18 of 245 dogs (7.3%), an ...	2016	27084481
evaluation of oxidant/antioxidant status, trace mineral levels, and erythrocyte osmotic fragility in goats naturally infected with anaplasma ovis.	anaplasma ovis, an arthropod-borne pathogen that infects erythrocytes, is the major cause of ovine and caprine anaplasmosis. this study was performed to assess in goats infected with a. ovis the osmotic fragility of erythrocytes, antioxidant status, and serum levels of microminerals. blood samples were collected from 104 mixed breed goats in ahvaz area, southwest iran and subjected to parasitologic, hematologic, oxidant/antioxidant, and micromineral assessment. anaplasma infection was detected i ...	2016	27142027
anaplasma marginale and theileria annulata in questing ticks from portugal.	ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...	2016	27394441
anaplasma, ehrlichia and rickettsial pathogens in ixodid ticks infesting cattle and sheep in western oromia, ethiopia.	although ticks are widely distributed in all agro-ecological zones of ethiopia, information on tick-borne pathogens is scarce. this study was conducted to determine the presence of anaplasma spp., ehrlichia spp., and rickettsia spp. in rhipicephalus evertsi and rhipicephalus (boophilus) decoloratus collected from cattle and sheep at bako, western oromia, ethiopia, using polymerase chain reaction and sequencing. anaplasma ovis and anaplasma spp., ehrlichia ruminantium and ehrlichia spp. were dete ...	2016	27411938
molecular and phylogenetic analysis of anaplasma spp. in sheep and goats from six provinces of china.	members of the genus anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. here, we investigated the presence of anaplasma spp. in 621 sheep and 710 goats from six provinces of china. polymerase chain reaction (pcr) and dna sequencing were conducted to determine the prevalence of anaplasma (a.) phagocytophilum, a. ovis and a. bovis targeting the 16s ribosomal rna or the major surface protein 4 gene. pcr revealed anaplasma in 39.0% (24 ...	2016	27456776
molecular study on infection rates of anaplasma ovis and anaplasma marginale in sheep and cattle in west-azerbaijan province, iran.	this study was carried out to determine the presence and frequency of anaplasma ovis and anaplasma marginale in sheep and dairy cattle in west-azerbaijan province, iran. a total number of 200 blood samples were randomly collected via the jugular vein from apparently healthy cattle (100) and sheep (100). the extracted dna from blood cells was screened using genus-specific (anaplasma spp.) nested-polymerase chain reaction (pcr) based on 16s rrna gene primer sets. species-specific pcr was set up us ...	2016	27482362
natural anaplasmataceae infection in rhipicephalus bursa ticks collected from sheep in the french basque country.	rhipicephalus bursa is one of 79 species of the genus rhipicephalus in the family of ixodidae. in this study, we investigated anaplasmataceae bacteria associated with r. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as r. bursa, all females were partially engorged. we found that 50% of the ticks were positive in the initial anaplasmataceae qpcr screening. bacterial species ...	2017	27666778
molecular detection and genetic characterization of babesia, theileria and anaplasma amongst apparently healthy sheep and goats in the central region of turkey.	babesia spp., theileria spp. and anaplasma spp. are significant tick-borne pathogens of livestock globally. in this study, we investigated the presence and distribution of babesia ovis, theileria ovis and anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the central anatolia region of turkey using species-specific pcr assays. the pcr were conducted using the primers based on the b. ovis ssu rrna (bossurrna), t. ovis ssu rrna (tossurrna) and a. ovis major surface pro ...	2017	27908771
evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china.	bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ...	2017	27942962
anaplasma species of veterinary importance in japan.	anaplasma species of the family anaplasmataceae, order rickettsiales are tick-borne organisms that can cause disease in animals and humans. in japan, all recognized species of anaplasma (except for anaplasma ovis) and a potentially novel anaplasma sp. closely related to anaplasma phagocytophilum have been reported. most of these detected tick-borne pathogens are believed to be lowly pathogenic in animals in japan although the zoonotic a. phagocytophilum has recently been reported to cause clinic ...	2016	27956767
guidelines for the direct detection of anaplasma spp. in diagnosis and epidemiological studies.	the genus anaplasma (rickettsiales: anaplasmataceae) comprises obligate intracellular gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: anaplasma bovis, anaplasma centrale, anaplasma marginale, anaplasma phagocytophilum, anaplasma platys, and anaplasma ovis. these have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. a zoonotic role has been recognized for a. phagocytophilum, but other spe ...	2017	28055579
a loop-mediated isothermal amplification (lamp) assay targeting 16s rrna gene for rapid detection of anaplasma phagocytophilum infection in sheep and goats.	anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever in various kinds of animals. in the present study, a loop-mediated isothermal amplification (lamp) assay for rapid detection of a. phagocytophilum was developed using primers specific to 16s rrna gene of this organism. the lamp assay was performed at 65 c for 60 min and terminated at 80 c for 10 min. the optimal reaction conditions, under which no cross ...	2017	28118097
molecular evidence of tick-borne hemoprotozoan-parasites (theileria ovis and babesia ovis) and bacteria in ticks and blood from small ruminants in northern algeria.	using qpcr, standard pcr and/or sequencing, we investigated the presence of tick-associated microorganisms in ticks and blood from sheep and goats from souk ahras, algeria. borrelia theileri, was detected in (7/120, 5.8%) blood from sheep and (13/120, 10.8%) goats. anaplasma ovis was screened in (38/73, 52%) rhipicephalus bursa and (5/22, 22.7%) r. turanicus and in (74/120, 61.7%), (65/120, 54.2%) blood of sheep and goats respectively. coxiella burnetii tested positive in r. bursa (4/73, 5.5%) a ...	2017	28131376
prevalence of tick-borne haemoparasites in small ruminants in turkey and diagnostic sensitivity of single-pcr and rlb.	tick-borne haemoparasitic diseases (tbhds), caused by theileria, babesia, anaplasma and ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. many of these diseases threaten livestock production and some also represent a concern to human public health. the primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) ...	2017	28449722
validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep.	an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ...	2017	28480803
molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake victoria and lake baringo, kenya.	although diverse tick-borne pathogens (tbps) are endemic to east africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. in particular, the role of reptiles in tbp epidemiology remains unknown, despite having been implicated with tbps of livestock among exported tortoises and lizards. understanding tbp ecologies, and the potential role of common reptiles, is critical for ...	2017	28620610
molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france.	corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ...	2017	28645313
molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock.	anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ...	2017	28678004
identification of anaplasma ovis appendage-associated protein (aaap) for development of an indirect elisa and its application.	ovine anaplasmosis is a tick-borne disease that is caused by anaplasma ovis in sheep and goats. the pathogen is widely distributed in tropical and subtropical regions of the world. at present, diagnosis of the disease mainly depends on microscopy or nucleic acid based molecular tests, although a few serological tests have been applied for the detection of a. ovis infection.	2017	28754151
molecular detection of pathogens in ticks infesting cattle in nampula province, mozambique.	ticks are ectoparasites that can act as vectors of a large number of pathogens in wild and domestic animals, pets, and occasionally humans. the global threat of emerging or re-emerging tick-borne diseases supports the need for research focused in the zoonotic transmission, especially in countries like mozambique where rural populations are in close contact with domestic animals. the present study aims to: (1) identify tick species infesting cattle from monapo and nacala porto, districts of nampu ...	2017	28856544