Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| investigating heavy-metal hyperaccumulation using thlaspi caerulescens as a model system. | metal-hyperaccumulating plant species are plants that are endemic to metalliferous soils and are able to tolerate and accumulate metals in their above-ground tissues to very high concentrations. one such hyperaccumulator, thlaspi caerulescens, has been widely studied for its remarkable properties to tolerate toxic levels of zinc (zn), cadmium (cd) and sometimes nickel (ni) in the soil, and accumulate these metals to very high levels in the shoot. the increased awareness regarding metal-hyperaccu ... | 2008 | 18440996 |
| plant response to heavy metal toxicity: comparative study between the hyperaccumulator thlaspi caerulescens (ecotype ganges) and nonaccumulator plants: lettuce, radish, and alfalfa. | thlaspi caerulescens (alpine pennycress) is one of the best-known heavy metal (hm) hyperaccumulating plant species. it exhibits the ability to extract and accumulate various hm at extremely high concentrations. in this hydroponic study, the performance of t. caerulescens (ecotype ganges) to accumulate cd, zn, and cu was compared with that of three nonaccumulator plants: alfalfa (medicago sativa), radish (raphanus sativus), and lettuce (lactuca sativa). plants were exposed to the separately disso ... | 2008 | 18528911 |
| investigation of heavy metal hyperaccumulation at the cellular level: development and characterization of thlaspi caerulescens suspension cell lines. | the ability of thlaspi caerulescens, a zinc (zn)/cadmium (cd) hyperaccumulator, to accumulate extremely high foliar concentrations of toxic heavy metals requires coordination of uptake, transport, and sequestration to avoid damage to the photosynthetic machinery. the study of these metal hyperaccumulation processes at the cellular level in t. caerulescens has been hampered by the lack of a cellular system that mimics the whole plant, is easily transformable, and competent for longer term studies ... | 2008 | 18550685 |
| does subcellular distribution in plants dictate the trophic bioavailability of cadmium to porcellio dilatatus (crustacea, isopoda)? | the present study examined how subcellular partitioning of cd in plants with different strategies to store and detoxify cd may affect trophic transfer of cd to the isopod porcellio dilatatus. the plant species used were lactuca sativa, a horticultural metal accumulator species; thlaspi caerulescens, a herbaceous hyperaccumulator species; and the nonaccumulator, t. arvense. taking into account that differences in subcellular distribution of cd in plants might have an important role in the bioavai ... | 2008 | 18624580 |
| feasibility of phytoextraction to remediate cadmium and zinc contaminated soils. | a cd and zn contaminated soil was mixed and equilibrated with an uncontaminated, but otherwise similar soil to establish a gradient in soil contamination levels. growth of thlaspi caerulescens (ganges ecotype) significantly decreased the metal concentrations in soil solution. plant uptake of cd and zn exceeded the decrease of the soluble metal concentrations by several orders of magnitude. hence, desorption of metals must have occurred to maintain the soil solution concentrations. a coupled regr ... | 2008 | 18644664 |
| the thlaspi caerulescens nramp homologue tcnramp3 is capable of divalent cation transport. | the nramp gene family encodes integral membrane protein and mediates the transport of fe, however, its function in transport of toxic metal ions is not very clear in plants. tcnramp3 was isolated from thlaspi caerulescens, and encoded a metal transporter member of the nramp family. tcnramp3 was predominantly expressed in roots of t. caerulescens by semi-quantitative rt-pcr. the expression of tcnramp3 was induced by iron starvation and by the heavy metals cd and ni in roots. tcnramp3 was able to ... | 2009 | 18663607 |
| the effect of nitrogen form on rhizosphere soil ph and zinc phytoextraction by thlaspi caerulescens. | the phytoextraction of zn may be improved by applying n fertilizers to increase the biomass and zn content of shoots. rhizosphere-ph change from uptake of different n forms will affect zn phyto-availability in the rhizosphere and zn phytoextraction. this glasshouse study examined the effect of n form on zn phytoextraction by thlaspi caerulescens (prayon). the plants were grown in a zn-contaminated soil (total zn 250 mg kg-1 soil; phwater 5.7) and supplied with (nh4)2so4, ca(no3)2 or urea [(nh2)2 ... | 2008 | 18752830 |
| chromosomal phylogeny and karyotype evolution in x=7 crucifer species (brassicaceae). | karyotype evolution in species with identical chromosome number but belonging to distinct phylogenetic clades is a long-standing question of plant biology, intractable by conventional cytogenetic techniques. here, we apply comparative chromosome painting (ccp) to reconstruct karyotype evolution in eight species with x=7 (2n=14, 28) chromosomes from six brassicaceae tribes. ccp data allowed us to reconstruct an ancestral proto-calepineae karyotype (pck; n=7) shared by all x=7 species analyzed. th ... | 2008 | 18836039 |
| metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to zn accumulation in the metal hyperaccumulator, thlaspi caerulescens. | to study the role of metallothioneins (mts) in zn accumulation, the expression of tcmt2a, tcmt2b, and tcmt3 was analysed in three accessions and 15 f(3) families of two inter-accession crosses of the cd/zn hyperaccumulator thlaspi caerulescens, with different degrees of zn accumulation. the highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from st laurent le minier, with >10-fold tcmt3 expression compared with another calamine accession and a ... | 2009 | 19033549 |
| cadmium sorption, influx, and efflux at the mesophyll layer of leaves from ecotypes of the zn/cd hyperaccumulator thlaspi caerulescens. | differential sorption and transport characteristics of the leaf mesophyll layer of the prayon and ganges ecotypes of the hyperaccumulator thlaspi caerulescens were examined. (109)cd influx and efflux experiments were conducted with leaf sections, and x-ray absorption near edge structure (xanes) data were collected from leaves as a general comparison of in vivo cadmium (cd) coordination. there were modest differences in cell wall sorption of cd between ecotypes. there were obvious differences in ... | 2009 | 19054336 |
| functional characterization of nramp3 and nramp4 from the metal hyperaccumulator thlaspi caerulescens. | the ability of metal hyperaccumulating plants to tolerate and accumulate heavy metals results from adaptations of metal homeostasis. nramp metal transporters were found to be highly expressed in some hyperaccumulating plant species. here, we identified tcnramp3 and tcnramp4, the closest homologues to atnramp3 and atnramp4 in thlaspi caerulescens and characterized them by expression analysis, confocal imaging and heterologous expression in yeast and arabidopsis thaliana. tcnramp3 and tcnramp4 are ... | 2009 | 19054339 |
| implications of metal accumulation mechanisms to phytoremediation. | trace elements (heavy metals and metalloids) are important environmental pollutants, and many of them are toxic even at very low concentrations. pollution of the biosphere with trace elements has accelerated dramatically since the industrial revolution. primary sources are the burning of fossil fuels, mining and smelting of metalliferous ores, municipal wastes, agrochemicals, and sewage. in addition, natural mineral deposits containing particularly large quantities of heavy metals are found in m ... | 2009 | 19067014 |
| thlaspi caerulescens (brassicaceae) population genetics in western switzerland: is the genetic structure affected by natural variation of soil heavy metal concentrations? | thlaspi caerulescens (brassicaceae) is a promising plant model with which to study heavy metal hyperaccumulation. population genetics studies are necessary for a better understanding of its history, which will be useful for further genomic studies on the evolution of heavy metal hyperaccumulation.the genetic structure of 24 natural swiss locations was investigated using nuclear and plastid loci. population genetics parameters were estimated and genetic pools were identified using bayesian infere ... | 2009 | 19076982 |
| model evaluation of the phytoextraction potential of heavy metal hyperaccumulators and non-hyperaccumulators. | evaluation of the remediation ability of zinc/cadmium in hyper- and non-hyperaccumulator plant species through greenhouse studies is limited. to bridge the gap between greenhouse studies and field applications for phytoextraction, we used published data to examine the partitioning of heavy metals between plants and soil (defined as the bioconcentration factor). we compared the remediation ability of the zn/cd hyperaccumulators thlaspi caerulescens and arabidopsis halleri and the non-hyperaccumul ... | 2009 | 19268408 |
| chelation by histidine inhibits the vacuolar sequestration of nickel in roots of the hyperaccumulator thlaspi caerulescens. | * the mechanisms of enhanced root to shoot metal transport in heavy metal hyperaccumulators are incompletely understood. here, we compared the distribution of nickel (ni) over root segments and tissues in the hyperaccumulator thlaspi caerulescens and the nonhyperaccumulator thlaspi arvense, and investigated the role of free histidine in ni xylem loading and ni transport across the tonoplast. * nickel accumulation in mature cortical root cells was apparent in t. arvense and in a high-ni-accumulat ... | 2009 | 19368671 |
| reciprocal grafting separates the roles of the root and shoot in zinc hyperaccumulation in thlaspi caerulescens. | * the extreme phenotype of zinc (zn) hyperaccumulation, which is found in several brassicaceae species, is determined by mechanisms that promote elevated zn tolerance and high zn accumulation in shoots. * we used reciprocal grafting between a zn hyperaccumulator, thlaspi caerulescens, and a zn nonaccumulator, thlaspi perfoliatum, to determine the relative importance of roots and shoots in zn hyperaccumulation and hypertolerance. * leaves from plants with a t. perfoliatum rootstock and a t. caeru ... | 2009 | 19656301 |
| complexation and toxicity of copper in higher plants. ii. different mechanisms for copper versus cadmium detoxification in the copper-sensitive cadmium/zinc hyperaccumulator thlaspi caerulescens (ganges ecotype). | the cadmium/zinc hyperaccumulator thlaspi caerulescens is sensitive toward copper (cu) toxicity, which is a problem for phytoremediation of soils with mixed contamination. cu levels in t. caerulescens grown with 10 microm cu(2+) remained in the nonaccumulator range (<50 ppm), and most individuals were as sensitive toward cu as the related nonaccumulator thlaspi fendleri. obviously, hyperaccumulation and metal resistance are highly metal specific. cu-induced inhibition of photosynthesis followed ... | 2009 | 19692532 |
| impacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment. | the effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (thlaspi caerulescens) grown in an artificially contaminated soil. the 16s ribosomal rna genes of the bacteria and the beta-proteobacteria and the amoa gene (encoding the alpha-subunit of ammonia monooxygenase) were pcr-amplified and analysed by denaturing gradient gel electrophoresis (dgge). principal component analysis (pca) of the dgge data reve ... | 2004 | 19712410 |
| transcriptional regulation of metal transport genes and mineral nutrition during acclimatization to cadmium and zinc in the cd/zn hyperaccumulator, thlaspi caerulescens (ganges population). | we investigated changes in mineral nutrient uptake and cellular expression levels for metal transporter genes in the cadmium (cd)/zinc (zn) hyperaccumulator, thlaspi caerulescens during whole plant and leaf ontogenesis under different long-term treatments with zn and cd. quantitative mrna in situ hybridization (qish) revealed that transporter gene expression changes not only dependent on metal nutrition/toxicity, but even more so during plant and leaf development. the main mrna abundances found ... | 2010 | 19843304 |
| decrease of labile zn and cd in the rhizosphere of hyperaccumulating thlaspi caerulescens with time. | by using a rhizobox micro-suction cup technique we studied in-situ mobilization and complexation of zn and cd in the rhizosphere of non-hyperaccumulating thlaspi perfoliatum and two different thlaspi caerulescens ecotypes, one of them hyperaccumulating zn, the other zn and cd. the dynamic fraction (free metal ions and small labile complexes) of zn and cd decreased with time in the rhizosphere solution of the respective hyperaccumulating t. caerulescens ecotypes, and at the end of the experiment, ... | 2010 | 19913965 |
| comparison of two ecotypes of the metal hyperaccumulator thlaspi caerulescens (j. & c. presl) at the transcriptional level. | this paper investigates differences in gene expression among the two thlaspi caerulescens ecotypes la calamine (lc) and lellingen (le) that have been shown to differ in metal tolerance and metal uptake. lc originates from a metalliferous soil and tolerates higher metal concentrations than le which originates from a non-metalliferous soil. the two ecotypes were treated with different levels of zinc in solution culture, and differences in gene expression were assessed through application of a cdna ... | 2010 | 19937357 |
| [stoichiometry of multi-elements in the zinc-cadmium hyperaccumulator thlaspi caerulescens grown hydroponically under different zinc concentrations determined by icp-aes]. | thlaspi caerulescens is commonly known as a zinc (zn) and cadmium (cd) hyperaccumulator, which can be used to clean up the zn- and/or cd-contaminated soil. however, it is unclear whether high soil zn concentrations will stimulate undue accumulations of other elements to such an extent as to cause the nutrient unbalance in the soil. to address this question, the inductively coupled plasma-atomic emission spectrometry (icp-aes) was employed to investigate the effect of zn on the stoichiometry of z ... | 2009 | 19950676 |
| interactions between plant and rhizosphere microbial communities in a metalliferous soil. | in the present work, the relationships between plant consortia, consisting of 1-4 metallicolous pseudometallophytes with different metal-tolerance strategies (thlaspi caerulescens: hyperaccumulator; jasione montana: accumulator; rumex acetosa: indicator; festuca rubra: excluder), and their rhizosphere microbial communities were studied in a mine soil polluted with high levels of cd, pb and zn. physiological response and phytoremediation potential of the studied pseudometallophytes were also inve ... | 2010 | 20036453 |
| proteomics of thlaspi caerulescens accessions and an inter-accession cross segregating for zinc accumulation. | metal hyperaccumulator plants have previously been characterized by transcriptomics, but reports on other profiling techniques are scarce. protein profiles of thlaspi caerulescens accessions la calamine (lc) and lellingen (le) and lines derived from an lcxle cross were examined here to determine the co-segregation of protein expression with the level of zinc (zn) hyperaccumulation. although hydrophobic proteins such as membrane transporters are not disclosed, this approach has the potential to r ... | 2010 | 20048332 |
| cloning and expression of brassica napus beta-carbonic anhydrase cdna. | a new full-length beta-carbonic anhydrase cdna was obtained from brassica napus by homologous cloning. the cdna has an open-reading frame of 996 nucleotides, encoding 331 amino acids with a calculated molecular weight of 35,692 da and an estimated pi value of 5.459. the deduced amino acid sequence of beta-carbonic anhydrase from brassica napus shared significant identity with beta-carbonic anhydrases from brassica carinata, arabidopsis thaliana, and thlaspi caerulescens (97.9%, 94%, and 93.5% id ... | 2009 | 20158161 |
| remediation of cadmium contaminated irrigation and drinking water: a large scale approach. | cadmium is one of the most troublesome toxic heavy metals. it accumulates in the water reservoirs and agricultural soil as a result of intensive use of cd contaminated phosphate fertilizers, e.g. in agriculture in the north central province (ncp) of sri lanka. the hyper-accumulator thlaspi caerulescens, accumulates up to 1000 ppm cd in shoots without exhibiting toxicity symptoms. the storage rhizomes of year old nelumbo nucifera (lotus) natural vegetation in water reservoirs in ncp accumulated 2 ... | 2010 | 20466045 |
| reciprocal and multi-species chromosome bac painting in crucifers (brassicaceae). | in crucifer cytogenomics, bac contigs of arabidopsis thaliana have been used as probes for comparative chromosome painting among species. here we successfully tested chromosome-specific bac contigs of a. thaliana (n = 5) and a. halleri (n = 8) as probes for reciprocal bac painting. furthermore, bac contigs of both arabidopsis species were applied as multi-species painting probes to a third crucifer species, noccaea caerulescens (n = 7), revealing their shared chromosome homeology. specifically, ... | 2010 | 20501976 |
| flow cytometric assessment of cd genotoxicity in three plants with different metal accumulation and detoxification capacities. | cadmium (cd) is a widespread environmental contaminant, strongly mutagenic and known to cause dna damage in plants. in this work, flow cytometry (fcm) was applied to determine if in vivo exposure to cd would induce genotoxic effects at the genome level. the hyper-accumulator thlaspi caerulescens (j. & c. presl), the related non-accumulator thlaspi arvense l. and the accumulator crop species lactuca sativa l. were germinated in distilled water and grown in modified hoagland's medium with increasi ... | 2010 | 20663557 |
| distinguishing diffusional and plant control of cd and ni uptake by hyperaccumulator and nonhyperaccumulator plants. | this work set out to test the hypothesis that uptake of metals by hyperaccumulator (ha) plants is more likely to be diffusion limited than uptake by nonhyperaccumulator (nha) plants. two circumneutral soils, with different contents of organic matter (0.8 and 5.8%), were amended with cd (0.5 to 5 mg kg(-1)) and ni (10 to 100 mg kg(-1)). a cd ha plant, thlaspi caerulescens, was grown in pots containing the cd amended soils, and a ni ha, thlaspi goesingense, was grown in pots containing the ni amen ... | 2010 | 20681510 |
| [heavy metal absorption, transportation and accumulation mechanisms in hyperaccumulator thlaspi caerulescens]. | thlaspi caerulescens, the famous model plant of heavy-metal hyperaccumulator, can uptake and accumulate large amount of heavy metals in its above-ground part of the plants. however, the very low biomass in thlaspi caerulescens makes this plant unfit for direct application in phytoremediation. in recent years, there are many reports about the physiological and molecular characterization of thlaspi caerulescens under heavy metals stresses, including absorption, transport and intracellular detoxifi ... | 2010 | 20684297 |
| metal hyperaccumulation armors plants against disease. | metal hyperaccumulation, in which plants store exceptional concentrations of metals in their shoots, is an unusual trait whose evolutionary and ecological significance has prompted extensive debate. hyperaccumulator plants are usually found on metalliferous soils, and it has been proposed that hyperaccumulation provides a defense against herbivores and pathogens, an idea termed the 'elemental defense' hypothesis. we have investigated this hypothesis using the crucifer thlaspi caerulescens, a hyp ... | 2010 | 20838462 |
| cadmium uptake and sequestration kinetics in individual leaf cell protoplasts of the cd/zn hyperaccumulator thlaspi caerulescens. | hyperaccumulators store accumulated metals in the vacuoles of large leaf epidermal cells (storage cells). for investigating cadmium uptake, we incubated protoplasts obtained from leaves of thlaspi caerulescens (ganges ecotype) with a cd-specific fluorescent dye. a fluorescence kinetic microscope was used for selectively measuring cd-uptake and photosynthesis in different cell types, so that physical separation of cell types was not necessary. few minutes after its addition, cadmium accumulated i ... | 2010 | 20880204 |
| impact of metal pollution and thlaspi caerulescens growth on soil microbial communities. | soil microorganisms drive critical functions in plant-soil systems. as such, various microbial properties have been proposed as indicators of soil functioning, making them potentially useful in evaluating the recovery of polluted soils via phytoremediation strategies. to evaluate microbial responses to metal phytoextraction using hyperaccumulators, a microcosm experiment was carried out to study the impacts of zn and/or cd pollution and thlaspi caerulescens growth on key soil microbial propertie ... | 2010 | 20935131 |
| generation of nonvernal-obligate, faster-cycling noccaea caerulescens lines through fast neutron mutagenesis. | noccaea caerulescens (formerly thlaspi caerulescens) is a widely studied metal hyperaccumulator. however, molecular genetic studies are challenging in this species because of its vernal-obligate biennial life cycle of 7-9months. here, we describe the development of genetically stable, faster cycling lines of n. caerulescens which are nonvernal-obligate. a total of 5500 m(0) seeds from saint laurent le minier (france) were subjected to fast neutron mutagenesis. following vernalization of young pl ... | 2010 | 21058953 |
| nicotianamine forms complexes with zn(ii) in vivo. | the non-proteinogenic amino acid nicotianamine (na) is a major player in plant metal homeostasis. it is known to form complexes with different transition metals in vitro. available evidence associates na with translocation of fe, and possibly other micronutrients, to and between different plant cells and tissues. to date, however, it is still extremely challenging to detect metal-ligand complexes in vivo because tissue disruption immediately changes the chemical environment and thereby the avail ... | 2010 | 21072375 |
| life history traits of the pseudometallophyte thlaspi caerulescens in natural populations from northern europe. | we examined recruitment, survival, life cycle and fecundity of two metallicolous (m, on metalliferous calamine soils) and two non-metallicolous (nm, on normal soils) populations of thlaspi caerulescens in belgium and luxemburg. in each population, permanent plots were monitored over two reproductive seasons. in m populations, plots were located in two contrasting environments (grass versus grove) in order to test the influence of vegetation cover on life strategy. our results show that the monoc ... | 2010 | 21134096 |
| response of antioxidative enzymes and apoplastic bypass transport in thlaspi caerulescens and raphanus sativus to cadmium stress. | a hydroponics experiment using hyperaccumulator thlaspi caerulescens (alpine pennycress) and non-specific accumulator raphanus sativus (common radish) was conducted to investigate the short-term effect of increasing cd concentrations (0, 25, 50, 75, 100 microm) on metal uptake, chlorophyll content, antioxidative enzymes, and apoplastic bypass flow. as expected, t. caerulescens generally showed better resistance to metal stress, which was reflected by higher cd accumulation within plant tissues w ... | 2010 | 21166344 |
| comparison of protein variations in thlaspi caerulescens populations from metalliferous and non-metalliferous soils. | in this work we analysed the protein variations which occurred in two thlaspi caerulescens populations when subjected to 0 and 10 microm nickel (ni) treatments: the ni hyperaccumulator t. caerulescensfrom a metalliferous soil in italy and t. caerulescens from czech republic, adapted to grow on a non-metalliferous soil. ni accumulation in roots and shoots and the effect on growth and morphology were examined. leaves proteins profiles of ni treated and untreated samples were analysed by two dimens ... | 2010 | 21166350 |
| phytochelatin synthase of thlaspi caerulescens enhanced tolerance and accumulation of heavy metals when expressed in yeast and tobacco. | phytochelatin synthase (pcs) is key enzyme for heavy metal detoxification and accumulation in plant. in this study, we isolated the pcs gene tcpcs1 from the hyperaccumulator thlaspi caerulescens. overexpression of tcpcs1 enhanced pc production in tobacco. cd accumulation in the roots and shoots of tcpcs1 transgenic seedlings was increased compared to the wild type (wt), while cd translocation from roots to shoots was not affected under cd treatment. the root length of the tcpcs1 transgenic tobac ... | 2011 | 21327392 |
| characterization of the glyoxalase 1 gene tcglx1 in the metal hyperaccumulator plant thlaspi caerulescens. | stress tolerance is currently one of the major research topics in plant biology because of the challenges posed by changing climate and increasing demand to grow crop plants in marginal soils. increased zn tolerance and accumulation has been reported in tobacco expressing the glyoxalase 1-encoding gene from brassica juncea. previous studies in our laboratory showed some zn tolerance-correlated differences in the levels of glyoxalase 1-like protein among accessions of zn hyperaccumulator thlaspi ... | 2011 | 21327818 |
| [effect of the soil bulk density on the root morphology and cadmium uptake by thlaspi caerulescens grown on cd-contaminated soil]. | a pot experiment was conducted using a soil contaminated with 2.12 mg x kg(-1) cd to study the effect of the variety of the soil bulk density on the zn/cd uptake by the hyperaccumulator thlaspi caerulescens and the removal of cd and zn from the soil. the contaminated soil received 0, 0.1%, 2% of soil conditioner and the plants were harvested after 100 days. the results showed that soil amendment with the soil conditioner (eb. a) significantly decreased the soil bulk density. compared to the cont ... | 2010 | 21360897 |
| isolation and characterization of arabidopsis halleri and thlaspi caerulescens phytochelatin synthases. | the synthesis of phytochelatins (pc) represents a major metal and metalloid detoxification mechanism in various species. pc most likely play a role in the distribution and accumulation of cd and possibly other metals. however, to date, no studies have investigated the phytochelatin synthase (pcs) genes and their expression in the cd-hyperaccumulating species. we used functional screens in two yeast species to identify genes expressed by two cd hyperaccumulators (arabidopsis halleri and thlaspi c ... | 2011 | 21369921 |
| isolation and expression analysis of partial sequences of heavy metal transporters from brassica juncea by coupling high throughput cloning with a molecular fingerprinting technique. | heavy metal transporters play a key role in regulating metal accumulation and transport in plants. these are important candidate genes to study in metal tolerant and accumulator plants for their potential use in environmental clean up. we coupled a degenerate primer-based rt-pcr approach with a molecular fingerprinting technique based on amplified rdna restriction analysis (ardra) to identify novel ests corresponding to heavy metal transporters from metal accumulator brassica juncea. we utilized ... | 2011 | 21394470 |
| tandem quadruplication of hma4 in the zinc (zn) and cadmium (cd) hyperaccumulator noccaea caerulescens. | zinc (zn) and cadmium (cd) hyperaccumulation may have evolved twice in the brassicaceae, in arabidopsis halleri and in the noccaea genus. tandem gene duplication and deregulated expression of the zn transporter, hma4, has previously been linked to zn/cd hyperaccumulation in a. halleri. here, we tested the hypothesis that tandem duplication and deregulation of hma4 expression also occurs in noccaea.a noccaea caerulescens genomic library was generated, containing 36,864 fosmid pcc1fos™ clones with ... | 2011 | 21423774 |
| elevated expression of tchma3 plays a key role in the extreme cd tolerance in a cd-hyperaccumulating ecotype of thlaspi caerulescens. | cadmium (cd) is a highly toxic heavy metal for plants, but several unique cd-hyperaccumulating plant species are able to accumulate this metal to extraordinary concentrations in the aboveground tissues without showing any toxic symptoms. however, the molecular mechanisms underlying this hypertolerance to cd are poorly understood. here we have isolated and functionally characterized an allelic gene, tchma3 (heavy metal atpase 3) from two ecotypes (ganges and prayon) of thlaspi caerulescens contra ... | 2011 | 21457363 |
| native plant communities in an abandoned pb-zn mining area of northern spain: implications for phytoremediation and germplasm preservation. | plants growing on metalliferous soils from abandoned mines are unique because of their ability to cope with high metal levels in soil. in this study, we characterized plants and soils from an abandoned pb-zn mine in the basque country (northern spain). soil in this area proved to be deficient in major macronutrients and to contain toxic levels of cd, pb, and zn. spontaneously growing native plants (belonging to 31 species, 28 genera, and 15 families) were botanically identified. plant shoots and ... | 2011 | 21598791 |
| biochemical and biophysical characterisation yields insights into the mechanism of a cd/zn transporting atpase purified from the hyperaccumulator plant thlaspi caerulescens. | tchma4 (genbank no. aj567384), a cd/zn transporting atpase of the p(1b)-type (=cpx-type) was isolated and purified from roots of the cd/zn hyperaccumulator thlaspi caerulescens. optimisation of the purification protocol, based on binding of the natural c-terminal his-tag of the protein to a ni-ida metal affinity column, yielded pure, active tchma4 in quantities sufficient for its biochemical and biophysical characterisation with various techniques. tchma4 showed activity with cu(2+), zn(2+) and ... | 2011 | 21621506 |
| application of rhizosphere interaction of hyperaccumulator noccaea caerulescens to remediate cadmium-contaminated agricultural soil. | there is an urgent requirement for selecting appropriate technologies to solve food safety problems due to soil contamination. in this study, the hyperaccumulator noccaea caerulescens and a high cd accumulator pakchoi cultivar (brassica rapa l. spp. chinenesis cv.) were grown in a moderately cd-contaminated soil with three planting systems (monocrop, inter-crop, and crop-rotation) and three growing durations (25, 50, and 75 days) to study the role of rhizosphere interaction of both species on th ... | 2011 | 21972514 |
| in situ assessment of phytotechnologies for multicontaminated soil management. | due to human activities, large volumes of soils are contaminated with organic pollutants such as polycyclic aromatic hydrocarbons, and very often by metallic pollutants as well. multipolluted soils are therefore a key concern for remediation. this work presents a long-term evaluation of the fate and environmental impact of the organic and metallic contaminants of an industrially polluted soil under natural and plant-assisted conditions. a field trial was followed for four years according to six ... | 2011 | 22046763 |