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[effect of uv-b radiation on release of nitrogen and phosphorus from leaf litter in subtropical region in china].the release of nitrogen and phosphorus from leaf litter of six representative species, cunninghamia lanceolata, pinus massoniana, schima superba, cinnamanun camphora, cyclobalanopsis glauca and castanopsis eyeri, was investigated with litterbag method under ambient and reduced uv-b radiation (22.1% below ambient) treatments in subtropical region. the results showed that, the n dynamics exhibited three patterns: immobilization, mineralization-immobilization and mineralization-immobilization-miner ...201222509595
[foliar water use efficiency and nitrogen use efficiency of dominant plant species in main forests along the north-south transect of east china].based on the measurements of the foliar carbon content (cmass, nitrogen content (nmass), isotope abundance (delta13c and delta15n), and light response curve of 10 dominant plant species (larix gmelinii, quercus mongolica, fraxinus mandshurica, tilia amurensis, acer mono, pinus koraiensis, cunninghamia lanceolata, schima superba, pinus massoniana, and castanea henryi) in the main forests along the north-south transect of eastern china (nstec), this paper analyzed the differences and the relations ...201222720598
effect of pinus massoniana lamb. bark extract on lytic cycle of epstein-barr virus.pinus massoniana bark extract (pmbe) at a concentration of 60 microg/ml or more inhibits the expression of epstein-barr virus (ebv) lytic proteins, such as rta, zta, and ea-d. ebv lytic cycle was blocked by inhibiting the transcription of immediate-early genes. the results suggest that the pmbe has anti-ebv activity. thus, the extract is potentially useful in preventing the lytic development of ebv in vitro.201223214264
effects of sulfated polysaccharide from masson pine (pinus massoniana) pollen on the proliferation and cell cycle of hepg2 cells.to explore the inhibitory effect of sulfated polysaccharide from masson pine (pinus massoniana) pollen (sppm60) on g2/m phase of human liver cancer hepg2 cells and its mechanism.201223270833
[root architecture and phosphorus efficiency of different provenance pinus massoniana under low phosphorous stress].taking four representative provenances of pinus massoniana from chun' an of zhejiang, wuping of fujian, cenxi of guangxi, and xinyi of guangdong in east and south china as test materials, a pot experiment was conducted to study their variations in root architecture and phosphorus (p) efficiency under heterogeneous and homogeneous low p stress. large variations were detected in the major growth traits and the indices of p efficiency among the p. massoniana provenances under both heterogeneous and ...201223285986
[fine root production and turnover in pinus massoniana plantation in three gorges reservoir area of china].by the methods of sequential soil cores and buried bags, an investigation was conducted to study the seasonal dynamics of fine roots in a 20-year-old pinus massoniana plantation in three gorges reservoir area from march to december 2011, with the annual production and turnover rate of the fine roots calculated. in the plantation, the annual mean biomass of <2 mm fine roots was 146.98 g x m(-2) x a(-1), in which, the living root biomass (102.92 g x m(-2) x a(-1)) was far greater than that of the ...201223285987
specific and functional diversity of endophytic bacteria from pine wood nematode bursaphelenchus xylophilus with different virulence.pine wilt disease (pwd) caused by the pine wood nematode (pwn), bursaphelenchus xylophilus, is one of the most devastating diseases of pinus spp. the pwn was therefore listed as one of the most dangerous forest pests in china meriting quarantine. virulence of the pwn is closely linked with the spread of pwd. however, main factors responsible for the virulence of pwns are still unclear. recently epiphytic bacteria carried by pwns have drawn much attention. but little is known about the relationsh ...201223289015
[relationships between soil moisture and needle-fall in masson pine forests in acid rain region of chongqing, southwest china].from march 2009 to november 2011, an investigation was conducted on the spatiotemporal variation of soil moisture and its effects on the needle-fall in masson pine (pinus massoniana) forests in acid rain region of chongqing, southeast china, with the corresponding soil moisture thresholds determined. no matter the annual precipitation was abundant, normal or less than average, the seasonal variation of soil moisture in the forests could be obviously divided into four periods, i.e., sufficient (b ...201223359920
effects of taishan pinus massoniana pollen polysaccharide on the subunit vaccine of proteus mirabilis in birds.three adjuvants, namely, taishan pinus massoniana pollen polysaccharide (tppps), white mineral oil (wo) and propolis (pp), were added to the outer membrane protein (omp) of proteus mirabilis (p. mirabilis) and their effects were compared. three hundred 1-day-old chicks were randomly divided into five groups (i-v), with 60 chicks per group, and injected subcutaneously with wo-omp vaccine (i), pp-omp vaccine (ii), tppps-omp vaccine (iii), omp-only vaccine (iv) and physiological saline (v) at 3, 7 ...201323403027
[effects of litterfall and root input on soil physical and chemical properties in pinus massoniana plantations in three gorges reservoir area, china].an investigation was made on the soil physical and chemical properties in different-aged pinus massoniana plantations in three gorges reservoir area under effects of litterfall and roots. the annual litter production in mature stand was 19.4% and 65.7% higher than that in nearly mature and middle-aged stands, respectively. the litter standing amount was in the sequence of mature stand > middle-aged stand > nearly mature stand, while the litter turnover coefficient was in the order of nearly matu ...201223479870
[spectral features analysis of pinus massoniana with pest of dendrolimus punctatus walker and levels detection].taking 51 field measured hyperspectral data with different pest levels in yanping, fujian province as objects, the spectral reflectance and first derivative features of 4 levels of healthy, mild, moderate and severe insect pest were analyzed. on the basis of 7 detecting parameters construction, the pest level detecting models were built. the results showed that (1) the spectral reflectance of pinus massoniana with pests were significantly lower than that of healthy state, and the higher the pest ...201323697126
characterization of the pinus massoniana transcriptional response to bursaphelenchus xylophilus infection using suppression subtractive hybridization.pine wilt disease (pwd) caused by pine wood nematode (pwn), bursaphelenchus xylophilus, is the most destructive diseases of pine and poses a threat of serious economic losses worldwide. although several of the mechanisms involved in disease progression have been discovered, the molecular response of pinus massoniana to pwn infection has not been explored. we constructed four subtractive suppression hybridization cdna libraries by taking time-course samples from pwn-inoculated masson pine trees. ...201323759987
taishan pinus massoniana pollen polysaccharides promote immune responses of recombinant bordetella avium ompa in balb/c mice.to study the effects of taishan pinus massoniana pollen polysaccharides (tppps) on bordetella avium outer membrane protein a (ompa) recombinant protein vaccine, ompa was expressed, confirmed by western blotting and mixed with tppps. female balb/c mice were randomly divided into six groups (i-vi). groups i, ii, and iii were treated with tppps-ompa at doses of 200, 400, and 800 mg/ml, respectively. groups iv, v, and vi were treated with freund's adjuvant-ompa, pure ompa, and physiological saline, ...201324076370
[characteristics of soil microbial biomass and community composition in three types of plantations in southern subtropical area of china].by using fumigation-extraction method and phospholipid fatty acids (plfas) analysis, this paper studied the characteristics of soil microbial biomass and community composition in the erythrophleum fordii, castanopsis hystrix, and pinus massoniana plantations in south subtropical china. the soil microbial biomass, total plfas, bacterial plfas, and fungal plfas in the plantations were significantly affected by the plantation type and season, and the soil microbial biomass, total plfas, and individ ...201324175505
studies on the causes of forest decline in nanshan, chongqing.nanshan is located in the south suburb of chongqing, sichuan province in china. it is about 10 km from the city of chongqing. there are about 2000 ha of forest, mainly masson pines (pinus massoniana lamb) which have exhibited decline since the beginning of the 1980s. surveys of stands estimated that 85% of masson pines are injured. symptomology of masson pines includes tip necrosis of needles, thin crown, reduced needle length, premature abscission, branch dieback, reduced radial growth. the fol ...199024243326
an improvement in sexual function is related to better quality of life, regardless of urinary function improvement: results from the idiprost® gold study.the relationship between lower urinary tract symptoms (luts) and erectile dysfunction (ed) has recently received increased attention. the aim of this study was to evaluate the efficacy of the alfa-5® association of serenoa repens, pinus massoniana bark extract (pmbe) and crocus sativus (idiprost® gold) in improvement of patient's quality of life, when compared with serenoa repens alone.201324399119
immunoregulatory effects of taishan pinus massoniana pollen polysaccharide on chicks co-infected with avian leukosis virus and bordetella avium early in ovo.in recent years, co-infection of chicken embryos with immunosuppressive viruses and bacteria occurs with an annually increasing frequency. consequently, studies on new and safe immunoregulators, especially plant polysaccharides, have become a popular topic in the poultry industry. in the present study, we selected 300 specific pathogen free embryonated eggs, which were injected with subgroup b avian leukosis virus (alv-b) and bordetella avium (b. avium) to establish an artificial co-infection mo ...201424508082
dentin biomodification potential depends on polyphenol source.although proanthocyanidins (pacs) modify dentin, the effectiveness of different pac sources and the correlation with their specific chemical composition are still unknown. this study describes the chemical profiling of natural pac-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (uhplc) to determine the overall composition of these extracts and, in parallel, comprehensively evaluate their effect on dentin properties. the total polyphenol content of the extr ...201424574140
effects of polysaccharide on chicks co-infected with bordetella avium and avian leukosis virus.chicks' co-infection with immunosuppressive virus and bacteria seriously threaten the development of the poultry industry. in this study, a model was established in which chicks were injected with either subgroup b alv (alv-b)+bordetella avium (b. avium), or alv-b+b. avium+taishan pinus massoniana pollen polysaccharide (tppps), or b. avium only, or b. avium+tppps. the data showed that the group injected with alv-b and b. avium exhibited significant inhibition of the immune function and therefore ...201424815403
a new phytophagous eulophid wasp (hymenoptera: chalcidoidea: eulophidae) that feeds within leaf buds and cones of pinus massoniana.aprostocetus pinus sp. nov. (chalcidoidea: eulophidae) is newly described as a leaf bud and microstrobilus pest of pinus massoniana (pinales: pinaceae), an important afforestation species in southeast china. both sexes of the parasitoid are described and illustrated.201424872309
new resinicolous chaenothecopsis species from china.four new resinicolous species of chaenothecopsis are described from china: chaenothecopsis perforata from exudate of rhus chinensis (anacardiaceae), c. pallida from exudate of ailanthus altissima (simaroubaceae), c. resinophila from exudate of kalopanax septemlobus (araliaceae) and c. hunanensis from resin of pinus massoniana (pinaceae). all the new species are compared with previously described resinicolous mycocalicioid taxa, and several new features in these species are presented. the newly d ...201424891410
protective immunity induced by the vaccination of recombinant proteus mirabilis ompa expressed in pichia pastoris.proteus mirabilis (p. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. to develop an effective vaccine to protect chickens against p. mirabilis infection, ompa, one of the major outer membrane proteins of p. mirabilis, was expressed in pichia pastoris. the concentration of the expressed recombinant ompa protein reached 8.0μg/ml after induction for 96h with 1.0% methanol in the culture. in addition, ompa protein was confirmed by sds-pa ...201525317910
immunomodulatory effects of taishan pinus massoniana pollen polysaccharide and propolis on immunosuppressed chickens.co-infection of reticuloendotheliosis virus (rev) and avian leukosis virus subgroup j (alv-j), which can cause suppressed immunity and vaccination failure, frequently occurs in chicken flocks in china. taishan pinus massoniana pollen polysaccharide (tppps) and propolis (pp) have been proven to possess immune modulatory effects and improve the immune effects of vaccines. this study aimed to investigate the immune modulatory ability of tppps and pp on chickens co-infected with immunosuppressive vi ...201525450885
inhibitory effects of pinus massoniana bark extract on hepatitis c virus in vitro.given that pinus massoniana lamb (pinaceae) bark extract (pmbe) is a safe and non-toxic flavonoid found abundantly in nature, it was considered a promising novel candidate agent in the treatment of virus infection.201525471218
quantitative classification and environmental interpretation of secondary forests 18 years after the invasion of pine forests by bursaphelenchus xylophilus (nematoda: aphelenchoididae) in china.with growing concerns over the serious ecological problems in pine forests (pinus massoniana, p. thunbergii) caused by the invasion of bursaphelenchus xylophilus (the pine wood nematode), a particular challenge is to determine the succession and restoration of damaged pine forests in asia. we used two-way indicator species analysis and canonical correlation analysis for the hierarchical classification of existing secondary forests that have been restored since the invasion of b. xylophilus 18 ye ...201425527600
isometric scaling of above- and below-ground biomass at the individual and community levels in the understorey of a sub-tropical forest.empirical studies and allometric partitioning (ap) theory indicate that plant above-ground biomass (ma) scales, on average, one-to-one (isometrically) with below-ground biomass (mr) at the level of individual trees and at the level of entire forest communities. however, the ability of the ap theory to predict the biomass allocation patterns of understorey plants has not been established because most previous empirical tests have focused on canopy tree species or very large shrubs.201525564468
characterization and biological activity of taishan pinus massoniana pollen polysaccharide in vitro.taishan pinus massoniana pollen polysaccharide (tppps) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. however, the components of tppps have not been recognized. to investigate the composition of tppps, crude polysaccharide was obtained from taishan p. massoniana pollen through water extraction and ethanol precipitation. three homogeneous polysaccharide fractions (tppps1, tppps2, and tppps3) were purified from tppps by deae-cellulose column chr ...201525782009
co-adjuvant effects of plant polysaccharide and propolis on chickens inoculated with bordetella avium inactivated vaccine.taishan pinus massoniana pollen polysaccharide (tppps), propolis (pp) and aloe polysaccharide (ap), used as adjuvants, have been proven to possess immunity-enhancing functions. however, their collaborative immunomodulatory effects are largely unknown. to determine which combination can induce the best effects, the three adjuvants were separately or conjointly added into bordetella avium inactivated vaccines to investigate their co-adjuvant effects on vaccinated chickens. we found that, among all ...201525989924
immunological and protective effects of bordetella bronchiseptica subunit vaccines based on the recombinant n-terminal domain of dermonecrotic toxin.dermonecrotic toxin (dnt) produced by bordetella bronchiseptica (b. bronchiseptica) can cause clinical turbinate atrophy in swine and induce dermonecrotic lesions in model mice. we know that the n-terminal of dnt molecule contains the receptor-binding domain, which facilitates binding to the target cells. however, we do not know whether this domain has sufficient immunogenicity to resist b. bronchiseptica damage and thereby to develop a subunit vaccine for the swine industry. in this study, we p ...201526337750
[an object-based information extraction technology for dominant tree species group types].information extraction for dominant tree group types is difficult in remote sensing image classification, howevers, the object-oriented classification method using high spatial resolution remote sensing data is a new method to realize the accurate type information extraction. in this paper, taking the jiangle forest farm in fujian province as the research area, based on the quickbird image data in 2013, the object-oriented method was adopted to identify the farmland, shrub-herbaceous plant, youn ...201526572017
morphology and molecular analysis of paratylenchus nanjingensis n. sp. (nematoda: paratylenchinae) from the rhizosphere soil of pinus massoniana in china.paratylenchus nanjingensis n. sp. was obtained from nanjing, jiangsu province, china. this new species is characterized by having a female with a slender, vermiform body (243-279 μm), head with distinct submedian lobes, slender and long stylet (64-68 μm), anchor-shaped stylet knobs, excretory pore anterior to the level of the stylet knobs, small lateral vulval flaps and lateral field with four lines; and male with more distinct body annuli, stylet lacking and pharynx degenerate. the internal tra ...201626821707
potentiation of taishan pinus massoniana pollen polysaccharide on the immune response and protection elicited by a highly pathogenic porcine reproductive and respiratory syndrome virus glycoprotein 5 subunit in pigs.porcine reproductive and respiratory syndrome virus (prrsv) heavily affects the global pork industry. current available vaccine strategies have inherent drawbacks. in this work, the immune enhancement from taishan pinus massoniana pollen polysaccharide (tppps) and freund's adjuvant on the efficacy of a prrsv subunit vaccine were examined. titers of specific anti-highly pathogenic prrsv (hp-prrsv) elisa antibody and neutralizing antibody were significantly higher in pigs from the groups inoculate ...201626828953
immune-enhancing effects of taishan pinus massoniana pollen polysaccharides on dna vaccine expressing bordetella avium ompa.bordetella avium is the causative agent of bordetellosis, which remains to be the cause of severe losses in the turkey industry. given the lack of vaccines that can provide good protection, developing a novel vaccine against b. avium infection is crucial. in this study, we constructed a eukaryotic expression plasmid, which expressed the outer membrane protein a (ompa) of b. avium, to prepare a b. avium recombinant ompa-dna vaccine. three concentrations (low, middle, and high) of taishan pinus ma ...201626870023
identification of steroleosin in oil bodies of pine megagametophytes.three classes of integral proteins termed oleosin, caleosin and steroleosin have been identified in seed oil bodies of diverse angiosperm species. recently, two oleosin isoforms and one caleosin were identified in megagametophyte oil bodies of pine (pinus massoniana), a representative gymnosperm species. in this study, a putative steroleosin of approximately 41 kda was observed in isolated oil bodies of pine megagametophytes, and its corresponding cdna fragment was obtained by pcr cloning and fu ...201626897709
carbon sequestration potential in stands under the grain for green program in southwest china.the grain for green program (ggp) is the largest afforestation and reforestation project in china in the early part of this century. to assess carbon sequestration in stands under the ggp in southwest china, the carbon stocks and their annual changes in the ggp stands in the region were estimated based on the following information: (1) collected data on the annually planted area of each tree species under the ggp in southwest china from 1999 to 2010; (2) development of empirical growth curves an ...201626959645
immuno-enhancement of taishan pinus massoniana pollen polysaccharides on recombinant bordetella avium ompa expressed in pichia pastoris.bordetellosis, caused by bordetella avium, continues to be an economic problem in the poultry industry of china. vaccines with good protective ability are lacking. thus, developing a novel vaccine against the b. avium infection is crucial. here, we constructed a recombinant pichia pastoris transformant capable of expressing the outer membrane protein a (ompa) of b. avium to prepare the recombinant ompa subunit vaccine and then evaluated its immune effects. to further investigate the immunomodula ...201626975477
[seasonal release characteristics of ca, mg and mn of foliar litter of six tree species in subtropical evergreen broadleaved forest].seasonal release dynamics of ca, mg and mn during decomposition of foliar litter of pinus massoniana, cryptomeria fortunei, cunninghamia lanceolata, cinnamomum camphora, toona ciliate, and quercus acutissima were investigated in subtropical evergreen broad-leaved forest employing the method of litterbag. after one-year decomposition, the release rates of ca, mg and mn in foliar litter of the studied tree species ranged from -13.8% to 92.3%, from 4.0% to 64.8%, and from 41.6% to 81.1%, respective ...201526995897
effect of biodegradation on thermogravimetric and chemical characteristics of hardwood and softwood by brown-rot fungus.the thermogravimetric and chemical characterization of hardwood eucalyptus urophylla (ep) and softwood pinus massoniana (mp) pretreated by brown-rot fungus gloeophyllum trabeum were investigated. the results indicated that the brown-rot fungus pretreatment can optimize the thermal decomposition and decrease the initiation temperatures (8-11°c lower) of both the ep and mp pyrolysis. the mean activation energy values of the bio-treated samples were 29.7kj/mol (for ep) and 42.3kj/mol (for mp) lower ...201627035476
dominance of ammonia-oxidizing archaea community induced by land use change from masson pine to eucalypt plantation in subtropical china.a considerable proportion of masson pine forests have been converted into eucalypt plantations in the last 30 years in guangdong province, subtropical china, for economic reasons, which may affect the ammonia-oxidizing archaea (aoa) community and the process of ammonia transformation. in order to determine the effects of forest conversion on aoa community, aoa communities in a masson pine (pinus massoniana) plantation and a eucalypt (eucalyptus urophylla) plantation, which was converted from the ...201627094186
efficacy of a formulation containing serenoa repens, crocus sativus and pinus massoniana extracts in men with concomitant luts and erectile dysfunction.the relationship between lower urinary tract symptoms (luts) and erectile dysfunction (ed) has received increased attention recently. this study aims to evaluate the efficacy of idiprost® gold, a product containing serenoa repens, crocus sativus and pinus massoniana bark extract (pmbe), in improving sexual function, urinary symptoms and quality of life in patients with concomitant luts and ed.201727583656
chicken igy fc linked to bordetella avium ompa and taishan pinus massoniana pollen polysaccharide adjuvant enhances macrophage function and specific immune responses.fc-fusion technologies, in which immunoglobulin fc is genetically fused to an antigenic protein, have been developed to confer antibody-like properties to proteins and peptides. mammalian igg fc fusion exhibits improved antigen-induced immune responses by providing aggregates with high avidity for the igg fc receptor and salvaging the antigenic portion from endosomal degradation. however, whether the linked chicken igy fc fragment shares similar characteristics to mammalian igg fc remains unclea ...201627847501
effect of forestry-waste biochars on adsorption of pb(ii) and antibiotic florfenicol in red soil.biochars derived from pinus massoniana and cunninghamia lanceolata trunks (abbreviated as pb and cb, respectively) were used to investigate their potential capabilities to improve lead (pb(ii)) and antibiotic florfenicol (flo) immobilization in soil. results shows that, after incubation for 60 days, the maximum adsorption capacities (q m ) of biochar-treated soils (soil-pb and soil-cb) for pb(ii) was increased by 27 and 14 %, respectively, compared with pristine soil sample. in the case of flo, ...201727900720
aliidongia dinghuensis gen. nov., sp. nov., a poly-β-hydroxybutyrate-producing bacterium isolated from pinus massoniana forest soil.a gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated 7m-z19t, was isolated from a soil sample collected from a pinus massoniana forest of dinghushan biosphere reserve, guangdong province, pr china. strain 7m-z19t grew at ph 4.5-7.5 (optimum ph 6.0-6.5), 10 to 37 °c (optimum 28 °c) and nacl concentration up to 2.0 % (optimum 0 %, w/v). iso-c17 : 0, c18 : 1ω7c and c19 : 0ω8c cyclo were the major fatty acids (>10 %) while ubiquinone-10 was the only respiratory quinone detected i ...201727902218
cloning of three genes involved in the flavonoid metabolic pathway and their expression during insect resistance in pinus massoniana lamb.pinus massoniana lamb. is an important timber and turpentine-producing tree species in china. dendrolimus punctatus and dasychira axutha are leaf-eating pests that have harmful effects on p. massoniana production. few studies have focused on the molecular mechanisms underlying pest resistance in p. massoniana. based on sequencing analysis of the transcriptomes of insect-resistant p. massoniana, three key genes involved in the flavonoid metabolic pathway were identified in the present study (pmf3 ...201628081282
cooperative effects of immune enhancer tppps and different adjuvants on antibody responses induced by recombinant alv-j gp85 subunit vaccines in spf chickens.to explore the antibody responses and protective effects induced by subgroup j avian leukosis virus (alv-j) gp85 protein vaccine plus different adjuvants (cpg and white oil adjuvant yf01) combined with the immune enhancer taishan pinus massoniana pollen polysaccharide (tppps), we immunized spf chickens with the recombinant alv-j gp85 protein, along with different adjuvants and immune enhancer, which protected the chickens by inducing different levels of protective antibodies. results showed that ...201728237498
taishan pinus massoniana pollen polysaccharide inhibits subgroup j avian leucosis virus infection by directly blocking virus infection and improving immunity.subgroup j avian leucosis virus (alv-j) generally causes neoplastic diseases, immunosuppression and subsequently increases susceptibility to secondary infection in birds. the spread of alv-j mainly depends on congenital infection and horizontal contact. although alv-j infection causes enormous losses yearly in the poultry industry worldwide, effective measures to control alv-j remain lacking. in this study, we demonstrated that taishan pinus massoniana pollen polysaccharide (tppps), a natural po ...201728287165
temporal variability of ammonia emission potentials for six plant species in an evergreen subtropical forest in southwest china.the temporal variability of leaf ammonia (nh3) emission potentials (the ratio of leaf tissue ammonium to proton concentration) and nitrogen (n) pools of six dominant plant species were investigated at the tieshanping (tsp) forested catchment, southwest china. the results showed that the nh3 emission potentials and n pools presented small variations among seasons, which were mainly controlled by plant species and the leaf age. also, high emission potential in one species did not correspond to hig ...201728290087
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