Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| development of ssr markers from citrus clementina (rutaceae) bac end sequences and interspecific transferability in citrus. | • premise of the study: microsatellite primers were developed from bacterial artificial chromosome (bac) end sequences of citrus clementina and their transferability and polymorphism tested in the genus citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. • methods and results: using page and dna silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in bes. a preliminary diversi ... | 2010 | 21616814 |
| diagnostic analysis of patients with essential hypertension using association rule mining. | the purpose of this study was to analyze the records of patients diagnosed with essential hypertension using association rule mining (arm). | 2010 | 21818427 |
| assessment of h1n1 questions and answers posted on the web. | background: a novel strain of human influenza a (h1n1) posed a serious pandemic threat worldwide during 2009. the public's fear of pandemic flu often raises awareness and discussion of such events. objectives: the goal of this study was to characterize major topical matters of h1n1 questions and answers raised by the online question and answer community yahoo! answers during h1n1 outbreak. methods: the study used text mining for spss clementine (v.12; spss inc., chicago, il) to extract the major ... | 2011 | 21840085 |
| cold treatment of ceratitis capitata (diptera: tephritidae) in oranges using a larval endpoint. | south africa currently exports fresh citrus (citrus spp.) fruit to japan using an in-transit cold treatment protocol of 14 d or 12 d at temperatures <0 degrees c for treatment of ceratitis capitata (wiedemann) (diptera: tephritidae) in 'clementine' mandarins (citrus reticulata blanco) and other citrus types, respectively. to reduce the risk of chilling injury with this treatment, research was conducted with temperatures >0 degrees c. earlier south african research had shown that young (6-d-old) ... | 2011 | 21882680 |
| characterization and comparison of volatile constituents of juice and peel from clementine, mandarin and their hybrids. | the volatile compositions of juice and peel of clementine (citrus reticulata x citrus sinensis var. commune), mandarin (citrus reticulata blanco var. willow leaf) and their hybrids were analyzed by headspace solid-phase extraction (hs-spme) coupled with gas chromatography and either a flame ionization detector (fid) or a mass spectrometer (ms). the major compounds of the volatile fractions of clementine and mandarin were limonene and limonene/gamma-terpinene, respectively. the volatile compositi ... | 2011 | 22164792 |
| exploiting bac-end sequences for the mining, characterization and utility of new short sequences repeat (ssr) markers in citrus. | the aim of this study was to develop a large set of microsatellite markers based on publicly available bac-end sequences (bess), and to evaluate their transferability, discriminating capacity of genotypes and mapping ability in citrus. a set of 1,281 simple sequence repeat (ssr) markers were developed from the 46,339 citrus clementina bac-end sequences (bes), of them 20.67% contained ssr longer than 20 bp, corresponding to roughly one perfect ssr per 2.04 kb. the most abundant motifs were di-nuc ... | 2011 | 22170603 |
| citrus juice extraction systems: effect on chemical composition and antioxidant activity of clementine juice. | clementines are especially appreciated for their delicious flavor, and recent years have seen a great increase in the consumption of clementine juice. in previous decades, antioxidant compounds have received particular attention because of widely demonstrated beneficial health effects. in this work, the organoleptic, volatile flavor, and antioxidant quality of clementine juice were studied with regard to the influence on them by different juice extraction systems: plug inside fruit and rotating ... | 2012 | 22225414 |
| snp mining in c. clementina bac end sequences; transferability in the citrus genus (rutaceae), phylogenetic inferences and perspectives for genetic mapping. | with the increasing availability of est databases and whole genome sequences, snps have become the most abundant and powerful polymorphic markers. however, snp chip data generally suffers from ascertainment biases caused by the snp discovery and selection process in which a small number of individuals are used as discovery panels. the ongoing international citrus genome consortium sequencing project of the highly heterozygous clementine and sweet orange genomes will soon result in the release of ... | 2012 | 22233093 |
| effect of fruit load on maturity and carotenoid content of clementine (citrus clementina hort. ex tan.) fruits. | citrus fruits contain many secondary metabolites displaying valuable health properties. there is a lot of interest in enhancing citrus quality traits, especially carotenoid contents, by agronomic approaches. in this study the influence of carbohydrate availability on maturity and quality criteria was investigated in clementine fruits during ripening. fruiting branches were girdled and defoliated after fruit set to obtain three levels of fruit load: high (five leaves per fruit), medium (15) and l ... | 2012 | 22311856 |
| comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of citrus clementina. | reproductive biology in citrus is still poorly understood. although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. here we report the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (citrus clementina hort. ex tan.). these genes have been identified comparing the transcriptomes of las ... | 2012 | 22333138 |
| a hybrid decision support model to discover informative knowledge in diagnosing acute appendicitis. | the aim of this study is to develop a simple and reliable hybrid decision support model by combining statistical analysis and decision tree algorithms to ensure high accuracy of early diagnosis in patients with suspected acute appendicitis and to identify useful decision rules. | 2012 | 22410346 |
| aroma characterization of tangerine hybrids by gas-chromatography-olfactometry and sensory evaluation. | tangerines have a distinct flavor among citrus fruit. however, information on tangerine volatiles remains limited. volatile compounds from a breeding population of tangerines were earlier identified by gas chromatography-mass spectrometry. in this study, five hybrids with a distinct volatile profile were analyzed by gas-chromatography-olfactometry (gc-o) and descriptive sensory analysis. | 2012 | 22413143 |
| authenticity of pgi "clementine of calabria" by multielement fingerprint. | clementine is a citrus fruit that has found a peculiar habitat in specific areas of calabria, a region located in southern italy. due to its peculiar characteristics it was recently awarded with protected geographical indications (pgi) from the european union. in this work, stepwise linear discriminant analysis (s-lda), soft independent modeling of class analogy (simca), and partial least-squares discriminant analysis (pls-da) were used to build chemometric models able to protect pgi clementine ... | 2012 | 22458691 |
| extensive citrus triploid hybrid production by 2x×4x sexual hybridizations and parent-effect on the length of the juvenile phase. | the citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. the recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. triploid plants can be recovered by 2x×4x sexual hybridization. in this work, we present an effective methodology to recover triploid plants from 2x×4x ... | 2012 | 22614256 |
| a quantitative diagnostic method for oral mucous precancerosis by rose bengal fluorescence spectroscopy. | a novel in vivo fluorescence spectroscopic diagnostic method has been developed in an animal model to make a quantified precancer diagnosis. in the study, 40 golden hamsters were randomly divided into four groups (groups a, b, c, and d), with group a being the control group and the other three groups being inducted at different precancer stages. a 1% rose bengal (rb) solution was used for the fluorescence spectroscopic diagnosis. a parameter k defined as k = i(rb)/i(auto) was introduced to refle ... | 2013 | 22648285 |
| citrus and prunuscopia-like retrotransposons. | many of the world's most important citrus cultivars ("washington navel", satsumas, clementines) have arisen through somatic mutation. this phenomenon occurs fairly often in the various species and varieties of the genus.the presence of copia-like retrotransposons has been investigated in fruit trees, especially citrus, by using a pcr assay designed to detect copia-like reverse transcriptase (rt) sequences. amplification products from a genotype of each the following species citrus sinensis, citr ... | 1999 | 22665184 |
| development of indel markers from citrus clementina (rutaceae) bac-end sequences and interspecific transferability in citrus. | indel markers were developed from bac-end sequences of citrus clementina cv. nules. transferability and polymorphism were tested in the citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. | 2012 | 22733984 |
| effects of chitosan coatings on physicochemical and nutritional quality of clementine mandarins cv. 'oronules'. | a commercial solution of chitosan was applied on mandarins 'oronules' at different solid content (0.6%, 1.2% and 1.8%). additionally, one group of mandarins was coated with a polyethylene-shellac commercial wax, and another group remained uncoated (control). mandarins were stored at 5 °c up to 28 days followed by 7 days at 20 °c simulating retail conditions. one group of mandarins was stored at 20 °c for 9 days simulating direct retail conditions. the commercial wax decreased weight and firmness ... | 2012 | 22763670 |
| carbohydrate control over carotenoid build-up is conditional on fruit ontogeny in clementine fruits. | the final contents of primary and secondary metabolites of the ripe fruit depend on metabolic processes that are tightly regulated during fruit ontogeny. carbohydrate supply during fruit development is known to influence these processes but, with respect to secondary metabolites, we do not really know whether this influence is direct or indirect. here, we hypothesized that the sensitivity of clementine fruit metabolism to carbohydrate supply was conditional on fruit developmental stage. we appli ... | 2013 | 22882610 |
| fruit load modulates flowering-related gene expression in buds of alternate-bearing 'moncada' mandarin. | gene determination of flowering is the result of complex interactions involving both promoters and inhibitors. in this study, the expression of flowering-related genes at the meristem level in alternate-bearing citrus trees is analysed, together with the interplay between buds and leaves in the determination of flowering. | 2012 | 22915579 |
| self-pollination and parthenocarpic ability in developing ovaries of self-incompatible clementine mandarins (citrus clementina). | this study aimed to determine if self-pollination is needed to trigger facultative parthenocarpy in self-incompatible clementine mandarins (citrus clementina hort. ex tan.). 'marisol' and 'clemenules' mandarins were selected, and self-pollinated and un-pollinated flowers from both cultivars were used for comparison. these mandarins are always seedless after self-pollination and show high and low ability to develop substantial parthenocarpic fruits, respectively. the time-course for pollen grain ... | 2013 | 23002897 |
| from cancer to cookbooks: the story of clementine paddleford. | clementine paddleford overcame laryngeal cancer to become the most famous culinary journalist of her time. through archival research, her long-forgotten tale may be shared anew. perhaps no individual better encapsulates the consequences of head and neck cancer than does a food reporter, for whom speech and swallowing are truly indispensable. in an era in which vocal rehabilitation after total laryngectomy was limited, and when conservation procedures were still being developed, paddleford underw ... | 2013 | 23064211 |
| international congress on transposable elements (icte) 2012 in saint malo and the sea of te stories. | an international conference on transposable elements (tes) was held 21-24 april 2012 in saint malo, france. organized by the french transposition community (gdr elements génétiques mobiles et génomes, cnrs) and the french society of genetics (sfg), the conference's goal was to bring together researchers from around the world who study transposition in diverse organisms using multiple experimental approaches. the meeting drew more than 217 attendees and most contributed through poster presentatio ... | 2012 | 23110759 |
| a reference genetic map of c. clementina hort. ex tan.; citrus evolution inferences from comparative mapping. | most modern citrus cultivars have an interspecific origin. as a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the international citrus genome consortium from a haploid derived from clementine mandarin. the availability of a saturated genetic map of clementine was identified as an essential prerequisite to assist the whole genome sequence assembly. clementine is believed to be a 'mediterranean' mandarin × sweet orange ... | 2012 | 23126659 |
| proteomic analysis of "moncada" mandarin leaves with contrasting fruit load. | a proteomic approach was used to know more about the molecular mechanism related to citrus alternate bearing. to this end, we researched protein expression differences between on-crop and off-crop "moncada" [clementine 'oroval' (citrus clementina hort ex tanaka) x 'kara' mandarin (citrus unshiu marc. x citrus nobilis lou.)] mandarin leaves. this variety usually shows a remarkable behaviour in alternate production. samples were collected in the period during which the fruit affect flowering induc ... | 2013 | 23202483 |
| citrus allergy from pollen to clinical symptoms. | allergy to citrus fruits is often associated with pollinosis and sensitization to other plants due to a phenomenon of cross-reactivity. the aims of the present study were to highlight the cross-reactivity among citrus and the major allergenic pollens/fruits, throughout clinical and molecular investigations, and to evaluate the sensitization frequency to citrus fruits in a population of children and adults with pollinosis. we found a relevant percentage of sensitisation (39%) to citrus fruits in ... | 2013 | 23308273 |
| evaluation of codon biology in citrus and poncirus trifoliata based on genomic features and frame corrected expressed sequence tags. | citrus, as one of the globally important fruit trees, has been an object of interest for understanding genetics and evolutionary process in fruit crops. meta-analyses of 19 citrus species, including 4 globally and economically important citrus sinensis, citrus clementina, citrus reticulata, and 1 citrus relative poncirus trifoliata, were performed. we observed that codons ending with a- or t- at the wobble position were preferred in contrast to c- or g- ending codons, indicating a close associat ... | 2013 | 23315666 |
| assignment of snp allelic configuration in polyploids using competitive allele-specific pcr: application to citrus triploid progeny. | polyploidy is a major component of eukaryote evolution. estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. with the increasing availability and whole-genome coverage of single nucleotide polymorphism (snp) markers, it is essential to implement a versatile snp genotyping method to assign allelic configuration efficiently in polyploids. | 2013 | 23422023 |
| [analysis of the characteristics of the older adults with depression using data mining decision tree analysis]. | the purpose of this study was to develop a prediction model for the characteristics of older adults with depression using the decision tree method. | 2013 | 23563063 |
| the citrus clementina putative allergens: from proteomic analysis to structural features. | several allergens have been identified and characterized in the genus citrus, which belongs to the germin-like proteins (gpls), profilins, and non-specific lipid transfer proteins (nsltps). in this work, in silico sequence analysis, protein purification, mass spectrometry identification, and the spectral counting method were integrated to identify new putative allergens of citrus clementina and their expression level in the fruit peel. the in silico analysis revealed fifteen new sequences belong ... | 2013 | 23927767 |
| [mining rules on determination of four properties based on traditional chinese medicine functional combination]. | it laid the foundation of the large sample data mining for a comprehensive summary concerning four properties theory of traditional chinese medicine (tcm), and also provided theory clues on determination of four properties for the new resource development of tcm and the clinical use of chinese medicine. | 2013 | 23947152 |
| comparative toxicity of pesticides in three phytoseiid mites with different life-style occurring in citrus: euseius stipulatus, neoseiulus californicus and phytoseiulus persimilis. | conservation and augmentative biological control strategies have been developed to take full advantage of the natural enemies that occur in spanish citrus orchards. among them, the predatory mites euseius stipulatus, neoseiulus californicus and phytoseiulus persimilis play an important role in the biological control of tetranychid mites. however, these predatory mites are often affected by pesticides and information about the side-effects of these products against these beneficial arthropods is ... | 2014 | 23979655 |
| expression profiling of two stress-inducible genes encoding for miraculin-like proteins in citrus plants under insect infestation or salinity stress. | the expression of two genes, namely mir1 and mir3 and the abundance of their encoded proteins, the putative miraculin-like proteins, mlp1 and mlp3, showing similarity to the kunitz family of protease inhibitors, were monitored in the leaves of the citrus variety, 'clementine' after tetranychus urticae infestation and elicitor treatments, or in the leaves of three other diploid citrus: 'willow leaf', 'cleopatra' mandarins and 'trifoliate' orange, as well as their respective doubled diploids and t ... | 2014 | 24001970 |
| cytological and molecular characterization of three gametoclones of citrus clementina. | three gametoclonal plants of citrus clementina hort. ex tan., cv. nules, designated esp, fra, and ita (derived from three labs in spain, france, and italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. the study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, ssr marker genotyping, and array-comparative genomic hy ... | 2013 | 24020638 |
| proteomic study of 'moncada' mandarin buds from on- versus off-crop trees. | a proteomic analysis of buds from mandarin trees with contrasting fruit load (on- and off-crop trees) was carried out during the onset of low-temperature induction. the aim of the study was to find out more about the molecular mechanism relating to alternate bearing in citrus and its relationship with flowering. the 'moncada' variety (clementine 'oroval'x'kara' mandarin), displaying remarkable behaviour in alternate production, was used in this study. from 2d dige gel, 192 spots were isolated: 9 ... | 2013 | 24056126 |
| coumarin and furanocoumarin quantitation in citrus peel via ultraperformance liquid chromatography coupled with mass spectrometry (uplc-ms). | coumarins and furanocoumarins are secondary metabolites commonly found in citrus plants. these molecules are allelochemical compounds in plants that have controversial effects on humans, such as phototoxicity and the commonly described interactions with drugs, referred to as the "grapefruit juice effect". thus, it is important to develop a reliable method to identify and quantitate the coumarins and furanocoumarins in citrus extracts. for this purpose, we herein describe an ultraperformance liqu ... | 2013 | 24117278 |
| economic threshold for tetranychus urticae (acari: tetranychidae) in clementine mandarins citrus clementina. | tetranychus urticae is a key pest of citrus in spain, especially of clementine mandarin trees. the effects of this mite on fruit production were assessed in 24 clementine trees for three consecutive years. trees were visited weekly and spider mite and phytoseiid mite populations and leaf flush patterns were estimated. at the end of the season, mandarins were harvested, weighed, and mite damage (scarring on the fruit) characterized. negative relationships between spider mite density and yield (kg ... | 2014 | 24122147 |
| characterization of the citrus genome through analysis of restriction fragment length polymorphisms. | studies on the nature of restriction fragment length polymorphisms (rflps) were undertaken to characterize the citrus genome. this type of analysis has not been carried out with any other perennial crop. citrus reticulata blanco cv clementine, c. xparadisi macf. cv duncan, and an f1 hybrid (lb 1-21) were used to determine what probe/enzyme combinations revealed polymorphisms in southern analysis, and a backcross family (lb 1-21x'clementine') of 65 randomly selected hybrid seedlings was used for ... | 1996 | 24166267 |
| mining of haplotype-based expressed sequence tag single nucleotide polymorphisms in citrus. | single nucleotide polymorphisms (snps), the most abundant variations in a genome, have been widely used in various studies. detection and characterization of citrus haplotype-based expressed sequence tag (est) snps will greatly facilitate further utilization of these gene-based resources. | 2013 | 24175923 |
| linkage of restriction fragment length polymorphisms and isozymes in citrus. | genetic linkage analysis was performed using two segregating populations of citrus. one population arose from an intergeneric backcross of citrus grandis (l.) osb. cv 'thong dee' and poncirus trifoliata (l.) raf. cv 'pomeroy', using the former as the recurrent (female) parent. the other population came from an interspecific backcross of c. reticulata blanco cv 'clementine' and c. x paradisi macf. cv 'duncan', using the former as the recurrent (male) parent. a total of 11 isozyme and 58 restricti ... | 1992 | 24203026 |
| development and characterization of genomic and expressed ssrs in citrus by genome-wide analysis. | microsatellites or simple sequence repeats (ssrs) are one of the most popular sources of genetic markers and play a significant role in plant genetics and breeding. in this study, we identified citrus ssrs in the genome of clementine mandarin and analyzed their frequency and distribution in different genomic regions. a total of 80,708 ssrs were detected in the genome with an overall density of 268 ssrs/mb. while di-nucleotide repeats were the most frequent microsatellites in genomic dna sequence ... | 2013 | 24204572 |
| cotyledonal chloroplasts in the hypogeal seeds of clementine. | clementine (citrus nobilisxcitrus aurantium amara pumila) is a chloroembryophyte with green quiescent embryos and hypogeal germination. the cotyledonal chloroplasts have been studied during germination in the dark and under two different irradiances 120 and 240 μmol·m(-2)·s(-1) throughout a period of three weeks. the plastids of the outer adaxial and inner regions develop differently. in the light, the former differentiate a photosynthetically active thylakoid system with an ultrastructural orga ... | 1987 | 24232958 |
| genetic structure of a phytophagous mite species affected by crop practices: the case of tetranychus urticae in clementine mandarins. | tetranychus urticae koch is a cosmopolitan mite considered as the most polyphagous species among spider mites. this mite is a key pest of clementine mandarins in eastern spain, where spanish clementine production concentrates. crop management practices can affect the population dynamics of this mite and, consequently, its impact on the orchard. microsatellite markers were used to study mite population genetics from two commercial orchards which had been managed differently following integrated p ... | 2014 | 24233157 |
| assessment of fruit juice authenticity using uplc-qtof ms: a metabolomics approach. | in recent years, with the growing complexity of global food supply chains and trade, food fraud, including adulteration of high value foods with cheaper substitutes, has become an increasingly important issue. a metabolomics approach can be applied to discover biomarkers that can be used to trace food adulteration. a study was undertaken to discover novel, potential biomarkers for the rapid detection of the adulteration of fruit juices with cheaper alternatives. pineapple, orange, grapefruit, ap ... | 2014 | 24262519 |
| building and mining web-based questionnaires and surveys with sysq. | a questionnaire is a method for collecting data that can come from many sources such as observations, telephone interviews or documentary sources. whatever the source of data is, the questionnaire provides a framework of questions that facilitate researcher's work. a manual approach for collecting data using questionnaire presents some limitations and introduces several sources of errors. a second issue regards the statistical and data mining of data that often is conducted using different tools ... | 2013 | 24307414 |
| buffering growth variations against water deficits through timely carbon usage. | water stresses reduce plant growth but there is no consensus on whether carbon metabolism has any role in this reduction. sugar starvation resulting from stomatal closure is often proposed as a cause of growth impairment under long-term or severe water deficits. however, growth decreases faster than photosynthesis in response to drought, leading to increased carbohydrate stores under short-term or moderate water deficits. here, we addressed the question of the role of carbon availability on grow ... | 2013 | 24348489 |
| altered sensitivity to ethylene in 'tardivo', a late-ripening mutant of clementine mandarin. | 'tardivo' mandarin is a mutant of 'comune' clementine with a delay in peel degreening and coloration, allowing late harvesting. in this work, we have explored if the late-harvesting phenotype of 'tardivo' mandarin is related to altered perception and sensitivity to ethylene. the peel degreening rate was examined after a single ethephon treatment or during a continuous ethylene application in fruits at two maturation stages. in general, ethylene-induced peel degreening was considerably delayed an ... | 2014 | 24372483 |
| [study of decision tree in the application of predicting protein-protein interactions]. | proteins are the final executive actor of cell viability and function. protein-protein interactions determine the complexity of the organism. research on the protein interactions can help us understand the function of the protein at the molecular level, learn the cell growth, development, differentiation, apoptosis and understand biological regulation mechanisms and other activities. they are essential for understanding the pathologies of diseases and helpful in the prevention and treatment of d ... | 2013 | 24459950 |
| [real-world study in analysis of effects on concomitant medications with parenterally administered shenmai for coronary heart disease]. | in order to understand the treatment of coronary heart disease with parenterally administered shenmai and the efficacy of combination therapies, the study selected 18 hospitals for analysis. data from each hospital's hospital injection system (his) was collected. data of in-patients receiving parenterally administered shenmai for a diagnosis of coronary heart disease was analyzed using; the apriori algorithm to model use, clementine 12.0 linkage analysis to find correlations between various drug ... | 2013 | 24471341 |
| why the aphid aphis spiraecola is more abundant on clementine tree than aphis gossypii? | aphis spiraecola and aphis gossypii cause harmful damages on clementine tree orchards. weekly surveys measured the abundance of aphids (larvae, winged and wingless adults) as well as of auxiliary insects and parameters of energy metabolism. correlatively, soluble carbohydrates, total free amino acids, free proline and condensed tannins were quantified in control and infested leaves. both aphid species showed parallel temporal variations, but a. spiraecola was consistently more abundant regardles ... | 2014 | 24581807 |
| knowledge discovery in a community data set: malnutrition among the elderly. | the purpose of this study was to design a prediction model that explains the characteristics of elderly adults at risk of malnutrition. | 2014 | 24627816 |
| n-methylated derivatives of tyramine in citrus genus plants: identification of n,n,n-trimethyltyramine (candicine). | the distribution of tyramine and its methylated derivatives, n-methyltyramine and n,n-dimethyltyramine, was investigated in tissue parts (leaves and fruits) of several plants of citrus genus by liquid chromatography-electrospray ionization tandem mass spectrometry (lc-esi-ms/ms). in the course of our study we discovered the occurrence of n,n,n-trimethyltyramine in all citrus plants examined. this quaternary ammonium compound, known to act in animals as a neurotoxin, was recognized and characteri ... | 2014 | 24635695 |
| low-hanging fruit: a clementine as a simulation model for advanced laparoscopy. | low-cost, high-fidelity models for training in advanced laparoscopic surgery techniques are not currently available. the objective of this study was to evaluate a model and assessment protocol for developing associated fine, precise laparoscopic dissection skills with accompanying surgical decision making. | 2014 | 24787562 |
| comprehensive analysis of ccch-type zinc finger gene family in citrus (clementine mandarin) by genome-wide characterization. | the ccch-type zinc finger proteins comprise a large gene family of regulatory proteins and are widely distributed in eukaryotic organisms. the ccch proteins have been implicated in multiple biological processes and environmental responses in plants. little information is available, however, about ccch genes in plants, especially in woody plants such as citrus. the release of the whole-genome sequence of citrus allowed us to perform a genome-wide analysis of ccch genes and to compare the identifi ... | 2014 | 24820208 |
| genetic diversity among mandarins in fruit-quality traits. | a detailed phenotypic analysis of fruit-quality traits was conducted among 46 mandarin varieties within the israeli citrus breeding collection, belonging to genetically different natural subgroups, including common mandarin (c. reticulata blanco), clementine (c. clementina hort. ex. tan), satsuma (c. unshiu marcovitch), mediterranean mandarin (c. deliciosa tenore), king mandarin (c. nobilis loureiro), and mandarin hybrids, such as tangor (c. reticulata × c. sinensis) and tangelo (c. reticulata × ... | 2014 | 24828369 |
| sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication. | cultivated citrus are selections from, or hybrids of, wild progenitor species whose identities and contributions to citrus domestication remain controversial. here we sequence and compare citrus genomes--a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes--and show that cultivated types derive from two progenitor species. although cultivated pummelos represent selections from one progenitor species, citrus maxima, cultivated mandarins ar ... | 2014 | 24908277 |
| the use of vis/nirs and chemometric analysis to predict fruit defects and postharvest behaviour of 'nules clementine' mandarin fruit. | the use of chemometrics to analyse vis/nirs signal collected from intact 'nules clementine' mandarin fruit at harvest, to predict the rind physico-chemical profile after eight weeks postharvest was explored. vis/nirs signals of 150 fruit were obtained immediately after harvest. reference data on the rind were obtained after eight-week storage, including colour index (ci), rind dry matter (dm), and concentration of sugars. partial least squares (pls) regression was applied to develop models. prin ... | 2014 | 24912725 |
| back propagation artificial neural network for community alzheimer's disease screening in china. | alzheimer's disease patients diagnosed with the chinese classification of mental disorders diagnostic criteria were selected from the community through on-site sampling. levels of macro and trace elements were measured in blood samples using an atomic absorption method, and neurotransmitters were measured using a radioimmunoassay method. spss 13.0 was used to establish a database, and a back propagation artificial neural network for alzheimer's disease prediction was simulated using clementine 1 ... | 2013 | 25206598 |
| transcriptome and metabolome analyses of sugar and organic acid metabolism in ponkan (citrus reticulata) fruit during fruit maturation. | ponkan (citrus reticulata blanco cv. ponkan) is an important mandarin citrus in china. however, the low ratio of sugars to organic acids makes it less acceptable for consumers. in this work, three stages (s120, early development stage; s195, commercial harvest stage; s205, delayed harvest stage) of ponkan fruit were selected for study. among 28 primary metabolites analyzed in fruit, sugars increased while organic acids in general decreased. rna-seq analysis was carried out and 19,504 genes were ... | 2015 | 25455100 |
| genome-wide analysis of citrus r2r3myb genes and their spatiotemporal expression under stresses and hormone treatments. | the r2r3myb proteins represent one of the largest families of transcription factors, which play important roles in plant growth and development. although genome-wide analysis of this family has been conducted in many species, little is known about r2r3myb genes in citrus, in this study, 101 r2r3myb genes has been identified in the citrus (citrus sinesis and citrus clementina) genomes, which are almost equal to the number of rice. phylogenetic analysis revealed that they could be subdivided into ... | 2014 | 25473954 |
| asymmetric reduction of ketones by biocatalysis using clementine mandarin (citrus reticulata) fruit grown in annaba or by ruthenium catalysis for access to both enantiomers. | biocatalytic reduction of prochiral ketones using freshly ripened clementine mandarin (citrus reticulata) in aqueous medium is reported. high enantioselectivities were observed, especially for the bioreduction of indanone , tetralone , and thiochromanone with respectively 95%, 99%, and 86% enantiomeric excess (ee). enantioselective bio- and metal-catalyzed reactions were compared. chiral ruthenium catalysts afforded good asymmetric inductions (>75% ee) in most cases, enantiomeric excesses depend ... | 2015 | 25482318 |
| relationship between soil temperature and fruit colour development of 'clemenpons' clementine mandarin (citrus clementina hort ex. tan). | in citrus, root temperature regulates rind colouration. however, few studies have investigated the range of temperatures and timing which determine rind colour break. the objective of this study was to determine the relationship between range of soil temperature (st) and rind colour development in the precocious ‘clemenpons’ clementine mandarin. reflective white plastic mulch was used to modify root temperature. | 2012 | 25520981 |
| [real world study of dengzhan xixin injection in treatment of cerebral infarction with medication]. | to analysis of dengzhan xixin injection (dzi) in treatment of cerebral infarction (ebhm) in the real world population characteristics and concomitant medication. by selecting the 20 hospital information system (his) used in the database of dzi and primary diagnosis of 2 484 cases of cerebral infarction patients information, use the apriori algorithm to construct the model, using clementine 12.0 analysis, cerebral infarction complicating diseases, commonly used drug combination analysis of dzi. t ... | 2014 | 25532393 |
| diversity in sensory quality and determining factors influencing mandarin flavor liking. | we evaluated the sensory quality of 42 mandarin varieties that belong to 7 different natural subgroups: common mandarin (citrus reticulata blanco), clementine (citrus clementina hort. ex. tan), satsuma (citrus unshiu marcovitch), mediterranean mandarin (citrus deliciosa tenore), king mandarin (citrus nobilis loureiro), and mandarin hybrids, such as tangor (citrus reticulata × citrus sinensis) and tangelo (citrus reticulata × citrus paradisi). consumer flavor acceptance tests revealed wide divers ... | 2015 | 25559381 |
| heat shock transcription factors expression during fruit development and under hot air stress in ponkan (citrus reticulata blanco cv. ponkan) fruit. | heat shock transcription factors (hsfs) play a role in plant responses to stress. citrus is an economically important fruit whose genome has been fully sequenced. so far, no detailed characterization of the hsf gene family is available for citrus. a genome-wide analysis was carried out in citrus clementina to identify hsf genes, named cchsfs. eighteen cchsfs were identified and classified into three main clades (clades a, b and c) according to the structural characteristics and the phylogenetic ... | 2015 | 25596345 |
| mapping the genetic and tissular diversity of 64 phenolic compounds in citrus species using a uplc-ms approach. | phenolic compounds contribute to food quality and have potential health benefits. consequently, they are an important target of selection for citrus species. numerous studies on this subject have revealed new molecules, potential biosynthetic pathways and linkage between species. although polyphenol profiles are correlated with gene expression, which is responsive to developmental and environmental cues, these factors are not monitored in most studies. a better understanding of the biosynthetic ... | 2015 | 25757470 |
| genome-wide comparative analysis reveals similar types of nbs genes in hybrid citrus sinensis genome and original citrus clementine genome and provides new insights into non-tir nbs genes. | in this study, we identified and compared nucleotide-binding site (nbs) domain-containing genes from three citrus genomes (c. clementina, c. sinensis from usa and c. sinensis from china). phylogenetic analysis of all citrus nbs genes across these three genomes revealed that there are three approximately evenly numbered groups: one group contains the toll-interleukin receptor (tir) domain and two different non-tir groups in which most of proteins contain the coiled coil (cc) domain. motif analysi ... | 2015 | 25811466 |
| diversity among mandarin varieties and natural sub-groups in aroma volatiles compositions. | mandarins constitute a large, diverse and important group within the citrus family. here, we analysed the aroma volatiles compositions of 13 mandarin varieties belonging to seven genetically different natural sub-groups that included common mandarin (c. reticulata blanco), clementine (c. clementina hort. ex. tan), satsuma (c. unshiu marcovitch), mediterranean mandarin (c. deliciosa tenore), king mandarin (c. nobilis loureiro), and mandarin hybrids, such as tangor (c. reticulata × c. sinensis) an ... | 2016 | 25824867 |
| genetic mapping of centromeres in the nine citrus clementina chromosomes using half-tetrad analysis and recombination patterns in unreduced and haploid gametes. | mapping centromere locations in plant species provides essential information for the analysis of genetic structures and population dynamics. the centromere's position affects the distribution of crossovers along a chromosome and the parental heterozygosity restitution by 2n gametes is a direct function of the genetic distance to the centromere. sexual polyploidisation is relatively frequent in citrus species and is widely used to develop new seedless triploid cultivars. the study's objectives we ... | 2015 | 25848689 |
| spatial and temporal spread of citrus tristeza virus and its aphid vectors in the north western area of morocco. | first report of citrus tristeza virus (ctv,closterovirus) in morocco datesback to 1961 in collections of citrus varieties. an exhaustive survey of citrus in the north of the country in 2009 revealed that ctv was spread all over the citrus production area. we attempted to evaluate the relative contribution of different aphid species in the spread of ctv disease in a citrus reticulata orchard at the loukkous region during 2 years (2012 and 2013). the overall ctv incidence estimated in the experime ... | 2016 | 25884375 |
| carotenoid bioaccessibility in pulp and fresh juice from carotenoid-rich sweet oranges and mandarins. | citrus fruits are a good source of carotenoids for the human diet; however, comparative studies of carotenoids in different citrus food matrices are scarce. in this work the concentration and bioaccessibility of carotenoids in sweet oranges and mandarins with marked differences in carotenoid composition were evaluated in pulp and compared to those in fresh juice. the pulp and juice of the red-fleshed cara cara sweet orange variety was highly rich in carotenes (mainly lycopene and phytoene) compa ... | 2015 | 25996796 |
| early embryo achievement through isolated microspore culture in citrus clementina hort. ex tan., cvs. 'monreal rosso' and 'nules'. | microspore embryogenesis is a method of achieving complete homozygosity from plants. it is particularly useful for woody species, like citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility. anther culture is currently the method of choice for microspore embryogenesis in many crops. however, isolated microspore culture is a better way to investigate the processes at the cellular, physiological, biochemical, and molecular levels as it avoids the i ... | 2015 | 26124764 |
| [mining analysis on composition and medication of menstruation prescriptions in fu qingzhu's obstetrics and gynecology]. | in this paper, menstruation prescriptions were selected from "fu qingzhu's obstetrics and gynecology" and analyzed by using gri algorithm, correlation analysis, hierarchical clustering method through spss, clementine and traditional chinese medicine (tcm) inheritance auxiliary systems, in order to screen out 15 menopathy prescriptions, which involve 45 traditional chinese medicine herbs. in the study, blood-tonifying and qi-tonifying herbs were found to be frequent in the prescriptions. the most ... | 2015 | 26281608 |
| presentation of a model-based data mining to predict lung cancer. | the data related to patients often have very useful information that can help us to resolve a lot of problems and difficulties in different areas. this study was performed to present a model-based data mining to predict lung cancer in 2014. | 2015 | 26411666 |
| degradation of imazalil, orthophenylphenol and pyrimethanil in clementine mandarins under conventional postharvest industrial conditions at 4°c. | the degradation of the postharvest fungicides imazalil, orthophenylphenol, and pyrimethanil was studied on clementine mandarins during packinghouse storage for a 28day period at 4°c. fruits to which orthophenylphenol was applied, were treated with imazalil and pyrimethanil at doses of 1000 and 2000mgl(-1), using cascade application for the later and cascade and wax for the former. the decay of the three fungicides was evaluated using an in-house validated analytical procedure that includes the e ... | 2016 | 26471663 |
| patterns of ambulatory dispersal in tetranychus urticae can be associated with host plant specialization. | dispersal can be an essential factor affecting the biological control of pests. tetranychus urticae koch (acari: tetranychidae) is a cosmopolitan and polyphagous species that may reach the pest status in many cropping systems including clementine orchards, where it may be found both in the trees and the associated flora. in a previous study, we demonstrated that the use of a ground cover of festuca arundinacea schreber (poaceae) offered a better regulation of t. urticae populations than traditio ... | 2016 | 26471983 |
| microspore embryogenesis through anther culture in citrus clementina hort. ex tan. | anther culture is a biotechnological method that allows to obtain, in one step, homozygous plants, very important to plant breeding, due to their numerous applications in mutation research, selection, genome sequencing, genetic analysis, and transformation. to induce the microspores, i.e., the immature male gametes, to switch from the normal gametophytic pathway to the sporophytic one, it is necessary to submit them to a type of stress, such as high or low temperature, starvation, or magnetic fi ... | 2016 | 26619882 |
| assessment of navel oranges, clementine tangerines, and rutaceous fruits as hosts of bactrocera cucurbitae and bactrocera latifrons (diptera: tephritidae). | export of citrus spp. fruits may require risk mitigation measures if grown in areas with established tephritid fruit fly (diptera: tephritidae) populations capable of infesting the fruits. the host status of citrus spp. fruits is unclear for two tephritid fruit fly species whose geographic ranges have expanded in recent years: melon fly, bactrocera cucurbitae (cocquillett), and bactrocera latifrons (hendel). in no choice cage infestation studies, b. latifrons oviposited into intact and punctured ... | 2015 | 26816484 |
| the usage of association rule mining to identify influencing factors on deafness after birth. | providing complete and high quality health care services has very important role to enable people to understand the factors related to personal and social health and to make decision regarding choice of suitable healthy behaviors in order to achieve healthy life. for this reason, demographic and clinical data of person are collecting, this huge volume of data can be known as a valuable resource for analyzing, exploring and discovering valuable information and communication. this study using foru ... | 2015 | 26862245 |
| inheritance in doubled-diploid clementine and comparative study with sdr unreduced gametes of diploid clementine. | tetraploid clementine displays mainly tetrasomic inheritance. genetic structures of 2n sdr and 2 × gametes from dd clementine are complementary and will guides triploids citrus breeding strategies. triploid breeding is developed worldwide to create new seedless cultivars. citrus triploid hybrids can be recovered from 2x × 2x sexual hybridizations as a consequence of the formation of unreduced gametes (2n), or from 4x × 2x interploid hybridizations in which tetraploid parents used are most often ... | 2016 | 27038940 |
| gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic citrus species. | citrus is a wide genus in which most of the cultivated species and cultivars are natural parthenocarpic mutants or hybrids (i.e. orange, mandarin, tangerine, grapefruit). the autonomous increase in ga1 ovary concentration during anthesis was suggested as being the stimulus responsible for parthenocarpy in citrus regardless of the species. to determine the exact ga-role in parthenocarpic fruit set, the following hypothesis was tested: ga triggers and maintains cell division in ovary walls causing ... | 2016 | 27095396 |
| the wrky transcription factor family in citrus: valuable and useful candidate genes for citrus breeding. | wrky transcription factors belong to a large family of plant transcriptional regulators whose members have been reported to be involved in a wide range of biological roles including plant development, adaptation to environmental constraints and response to several diseases. however, little or poor information is available about wrky's in citrus. the recent release of completely assembled genomes sequences of citrus sinensis and citrus clementina and the availability of ests sequences from other ... | 2016 | 27193354 |
| [effect of chinese herbs used in treating multiple sclerosis on t subsets using association rules]. | to analyze the effect of chinese herbs used by prof. li tao on peripheral blood t subsets in treating multiple sclerosis (ms) by using association rules and statistical methods, thereby providing evidence for optimizing prescriptions. | 2016 | 27323613 |
| lrr-rlk family from two citrus species: genome-wide identification and evolutionary aspects. | leucine-rich repeat receptor-like kinases (lrr-rlks) represent the largest subfamily of plant rlks. the functions of most lrr-rlks have remained undiscovered, and a few that have been experimentally characterized have been shown to have important roles in growth and development as well as in defense responses. although rlk subfamilies have been previously studied in many plants, no comprehensive study has been performed on this gene family in citrus species, which have high economic importance a ... | 2016 | 27515968 |
| clementine juice has the potential for drug interactions - in vitro comparison with grapefruit and mandarin juice. | adverse drug interactions due to grapefruit juice are well known prompting warnings even in drug labels. similar issues have not been reported for clementines and available data is scarce, despite of genetic descent. we observed substantially increased tacrolimus trough concentrations in a renal transplant patient consuming high clementine amounts and, thus, scrutinised the effects of clementine juice on drug metabolism and drug transporters in vitro and compared it to the effects of mandarin an ... | 2017 | 27890698 |
| genome-wide identification and functional analysis of s-rnase involved in the self-incompatibility of citrus. | s-rnase-based self-incompatibility is found in solanaceae, rosaceae, and scrophulariaceae, and is the most widespread mechanism that prevents self-fertilization in plants. although 'shatian' pummelo (citrus grandis), a traditional cultivated variety, possesses the self-incompatible trait, the role of s-rnases in the self-incompatibility of 'shatian' pummelo is poorly understood. to identify genes associated with self-incompatibility in citrus, we identified 16 genes encoding homologs of ribonucl ... | 2017 | 27933381 |
| effect of postharvest practices including degreening on citrus carpoplane microbial biomes. | to investigate the effect of commercial citrus packhouse processing steps on the fruit surface microbiome of clementines and palmer navel oranges. | 2017 | 28052466 |
| differential diagnosis of erythmato-squamous diseases using classification and regression tree. | differential diagnosis of erythmato-squamous diseases (esd) is a major challenge in the field of dermatology. the esd diseases are placed into six different classes. data mining is the process for detection of hidden patterns. in the case of esd, data mining help us to predict the diseases. different algorithms were developed for this purpose. | 2016 | 28077889 |
| influence of orange cultivar and mandarin postharvest storage on polyphenols, ascorbic acid and antioxidant activity during gastrointestinal digestion. | polyphenols, ascorbic acid content and antioxidant activity of two sweet oranges (navel-n and cara cara-cc) and mandarin (clementine-m) as well as their bioaccessibilities were evaluated in pulps and compared to those in fresh juice. thus, pulps of oranges and mandarins displayed higher hesperidin (hes), narirutin (nar), total flavonoids (tf), total phenols (tp) and antioxidant activity (aac) than their corresponding juices. also, cc products presented higher bioactive compounds content than n o ... | 0 | 28193404 |
| changes in anthocyanin production during domestication of citrus. | mandarin (citrus reticulata), citron (citrus medica), and pummelo (citrus maxima) are important species of the genus citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to citrus, but mandarins do not, and pummelo varieties that produce anthocyanins have not been repo ... | 2017 | 28196843 |
| experimental evidence and in silico identification of tryptophan decarboxylase in citrus genus. | plant tryptophan decarboxylase (tdc) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. the recent finding of tryptamine metabolites in citrus plants leads to hypothesize the existence of tdc activity in this genus. here, we report for the first time that, in citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successively deuterated n,n,n-trimethyltryptamine is formed. these results give an evidence of the occurrence of ... | 2017 | 28208655 |
| characterization of botanical and geographical origin of corsican "spring" honeys by melissopalynological and volatile analysis. | pollen spectrum, physicochemical parameters and volatile fraction of corsican "spring" honeys were investigated with the aim of developing a multidisciplinary method for the qualification of honeys in which nectar resources are under-represented in the pollen spectrum. forty-one corsican "spring" honeys were certified by melissopalynological analysis using directory and biogeographical origin of 50 representative taxa. two groups of honeys were distinguished according to the botanical origin of ... | 2014 | 28234308 |
| comprehensive comparative analysis of volatile compounds in citrus fruits of different species. | the volatile profiles of fruit peels and juice sacs from 108 citrus accessions representing seven species were analyzed. using gc-ms 162 and 107 compounds were determined in the peels and juice sacs, respectively. in the peels, monoterpene alcohols were accumulated in loose-skin mandarins; clementine tangerines and papedas were rich in sesquiterpene alcohols, sesquiterpenes, monoterpene alcohols and monoterpene aldehydes. β-pinene and sabinene were specifically accumulated in 4 of 5 lemon germpl ... | 0 | 28407917 |
| alternative oxidase (aox) constitutes a small family of proteins in citrus clementina and citrus sinensis l. osb. | the alternative oxidase (aox) protein is present in plants, fungi, protozoa and some invertebrates. it is involved in the mitochondrial respiratory chain, providing an alternative route for the transport of electrons, leading to the reduction of oxygen to form water. the present study aimed to characterize the family of aox genes in mandarin (citrus clementina) and sweet orange (citrus sinensis) at nucleotide and protein levels, including promoter analysis, phylogenetic analysis and c. sinensis ... | 2017 | 28459876 |
| tetraploid carrizo citrange rootstock (citrus sinensis osb.×poncirus trifoliata l. raf.) enhances natural chilling stress tolerance of common clementine (citrus clementina hort. ex tan). | low temperatures can disturb the development, growth and geographic distribution of plants, particularly cold-sensitive plants in the mediterranean area, where temperatures can reach seasonally low levels. in citrus crops, scion/rootstock combinations are used to improve fruit production and quality, and increase tolerance to biotic and abiotic stresses. in the last decade, several studies have shown that tetraploid citrus seedlings or rootstocks are more tolerant to abiotic stress than their re ... | 2017 | 28478318 |
| long-term effect of a leonardite iron humate improving fe nutrition as revealed in silico, in vivo, and in field experiments. | novel, cheap and ecofriendly fertilizers that solve the usual iron deficiency problem in calcareous soil are needed. the aim of this work is to study the long-term effect of an iron leonardite fertilizer on citrus nutrition taking into account a properly characterization, kinetic response with a ligand competition experiment, efficiency assessment using saccharomyces cerevisiae strain and finally, in field conditions with citrus as test plants. its efficiency was compared with the synthetic iron ... | 2017 | 28715216 |
| comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes. | citrus fruit flavor is an important attribute prioritized in variety improvement. the present study compared juice volatiles compositions from 13 selected citrus genotypes, including six mandarins (citrus reticulata), three sour oranges (citrus aurantium), one blood orange (citrus sinensis), one lime (citrus limonia), one clementine (citrus clementina) and one satsuma (citrus unshiu). | 2018 | 28731231 |
| profiles of free and bound phenolics extracted from citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties. | this study of selected plants of the rutaceae family was carried out to investigate their phenolic content, antioxidant activity, and the in vitro inhibitory potential of extracted phenolics towards enzymes relevant for hyperglycemia and hypertension. the phenolic content, antioxidant activity and phenolic extract-mediated inhibitory activities for α-glucosidase and α-amylase were evaluated by spectrophotometry. the content of individual phenolics and the angiotensin i-converting enzyme (ace) in ... | 2017 | 28805834 |
| [real world analysis to explore clinical features of shenxiong glucose injection combined with other medications]. | to explore the real world clinical medication and combination characteristics of shenxiong glucose injection. the basic information of patients with shenxiong glucose injection, traditional chinese and western medicine diagnosis information, doctor advice information and laboratory test information from the hospital information system(his) of 19 tertiary hospitals in china. apriori algorithm was adopted to establish the models, and clementine 12.0 was used for correlation analysis to analyze the ... | 2017 | 28945045 |
| prlpaat4, a putative lysophosphatidic acid acyltransferase from paeonia rockii, plays an important role in seed fatty acid biosynthesis. | lysophosphatidic acid acyltransferases (lpaats) are essential for the acylation of lysophosphatidic acid (lpa) and the synthesis of phosphatidic acid (pa), a key intermediate in the synthesis of membrane phospholipids and storage lipids. here, a putative lysophosphatidic acid acyltransferase gene, designated prlpaat4, was isolated from seed unsaturated fatty acid (ufa)-rich p. rockii. the complete prlpaat4 cdna contained a 1116-bp open reading frame (orf), encoding a 42.9 kda protein with 371 am ... | 2017 | 28994730 |