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bioactive phytochemicals from wild arbutus unedo l. berries from different locations in portugal: quantification of lipophilic components.the lipophilic composition of wild arbutus unedo l. berries, collected from six locations in penacova (center of portugal), as well as some general chemical parameters, namely total soluble solids, ph, titratable acidity, total phenolic content and antioxidant activity was studied in detail to better understand its potential as a source of bioactive compounds. the chemical composition of the lipophilic extracts, focused on the fatty acids, triterpenoids, sterols, long chain aliphatic alcohols an ...201526110390
contrasting impacts of continuous moderate drought and episodic severe droughts on the aboveground-biomass increment and litterfall of three coexisting mediterranean woody species.climate change is predicted to increase the aridity in the mediterranean basin and severely affect forest productivity and composition. the responses of forests to different timescales of drought, however, are still poorly understood because extreme and persistent moderate droughts can produce nonlinear responses in plants. we conducted a rainfall-manipulation experiment in a mediterranean forest dominated by quercus ilex, phillyrea latifolia, and arbutus unedo in the prades mountains in souther ...201526149833
mycorrhizal synthesis between lactarius deliciosus and arbutus unedo l.arbutoid mycorrhizae were synthesized in vitro between arbutus unedo l. and two isolates of lactarius deliciosus. the fungal isolates were obtained from sporocarps collected under pinus sylvestris and in a mixed forest stand of quercus suber and pinus pinea. synthesis tubes filled with a mixture of sterilized peat, vermiculite, and perlite imbibed with nutrient solution were used. two inoculation methods using solid and liquid media were tested. shoots from an adult selected clone of a. unedo we ...201626215114
low among-provenance differences in structural and functional plasticity in response to nutrients in saplings of the circum-mediterranean tree arbutus unedo l.the mediterranean region is an area of special interest for conservation where the incidence of multiple drivers of global change is expected to increase. one of the factors predicted to change is soil-nutrient availability, an essential factor for plant growth. thus, study of the effects of variation in this parameter is especially relevant in species with a circum-mediterranean distribution, such as arbutus unedo l., in which the different provenances grow in different habitats, which must dif ...201526377872
quantitative analysis of arbutin and hydroquinone in strawberry tree (arbutus unedo l., ericaceae) leaves by gas chromatography-mass spectrometry.the phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. in this study, an extraction procedure coupled with gas chromatography-mass spectrometry (gc-ms) was developed to determine arbutin and hydroquinone content in strawberry tree (arbutus unedo l., ericaceae) leaves. the method showed good linearity (r2>0.9987) in the tested concentration range (0.5-200 μg ml(-1)), as well as good precision ...201526444340
effects of methanol extracts from roots, leaves, and fruits of the lebanese strawberry tree (arbutus andrachne) on cardiac function together with their antioxidant activity.several plant-derived natural products have been used in clinical phase for applications in neurological, cardiovascular, and inflammatory diseases. arbutus andrachne l. (ericacea) is an evergreen shrub native to the mediterranean region. traditionally, the fruits and leaves of arbutus tree are well known and used as antiseptics, diuretics, blood tonic, and laxatives.201626511200
interactive effects of uv radiation and reduced precipitation on the seasonal leaf phenolic content/composition and the antioxidant activity of naturally growing arbutus unedo plants.the effects of uv radiation and rainfall reduction on the seasonal leaf phenolic content/composition and antioxidant activity of the mediterranean shrub arbutus unedo were studied. naturally growing plants of a. unedo were submitted to 97% uv-b reduction (uva), 95% uv-a+uv-b reduction (uv0) or near-ambient uv levels (uvba) under two precipitation regimes (natural rainfall or 10-30% rainfall reduction). total phenol, flavonol and flavanol contents, levels of eight phenols and antioxidant activity ...201526562808
somatic embryogenesis induction and plant regeneration in strawberry tree (arbutus unedo l.).somatic embryogenesis is a powerful tool both for cloning and studies of genetic transformation and embryo development. most protocols for somatic embryogenesis induction start from zygotic embryos or embryonic-derived tissues which do not allow the propagation of elite trees. in the present study, a reliable protocol for somatic embryogenesis induction from adult trees of strawberry tree is described. leaves from in vitro proliferating shoots were used to induce somatic embryo formation on a me ...201626619869
phylogeography of a widespread species: pre-glacial vicariance, refugia, occasional blocking straits and long-distance migrations.phylogeographic studies give us the opportunity to reconstruct the historical migrations of species and link them with climatic and geographic variation. they are, therefore, a key tool to understanding the relationships among biology, geology and history. one of the most interesting biogeographical areas of the world is the mediterranean region. however, in this area, the description of concordant phylogeographic patterns is quite scarce, which limits the understanding of evolutionary patterns ...201626768603
carotenoid composition of strawberry tree (arbutus unedo l.) fruits.the carotenoid composition of strawberry tree (arbutus unedo) fruits has been characterised in detail and quantified for the first time. according to the total carotenoid content (over 340 μg/g dw), mature strawberry tree berries can be classified as fruits with very high carotenoid content (>20 μg/g dw). (all-e)-violaxanthin and 9z-violaxanthin were found to be the major carotenoid pigments, accounting for more than 60%, responsible for the bright colour of the flesh of ripe fruits. in addition ...201626775958
population structure of a widespread species under balancing selection: the case of arbutus unedo l.arbutus unedo l. is an evergreen shrub with a circum-mediterranean distribution that also reaches the eurosiberian region in northern iberia, atlantic france, and a disjunct population in southern ireland. due to the variety of climatic conditions across its distribution range, the populations of a. unedo were expected to display local adaptation. conversely, common garden experiments revealed that diverse genotypes from a range of provenances produce similar phenotypes through adaptive plastici ...201526793231
pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method.many fruits soften during ripening, which is important commercially and in rendering the fruit attractive to seed-dispersing animals. cell-wall polysaccharide hydrolases may contribute to softening, but sometimes appear to be absent. an alternative hypothesis is that hydroxyl radicals ((•)oh) non-enzymically cleave wall polysaccharides. we evaluated this hypothesis by using a new fluorescent labelling procedure to 'fingerprint' (•)oh-attacked polysaccharides.201626865506
stability and metabolism of arbutus unedo bioactive compounds (phenolics and antioxidants) under in vitro digestion and colonic fermentation.the natural antioxidants of arbutus unedo highlight the importance of this fruit as natural source of bioactive compounds. in the present study, to evaluate the stability of phenolic compounds, ascorbic acid and fat-soluble antioxidants (α-tocopherol, β-carotene and lutein), in vitro gastrointestinal digestion was applied to a. unedo fruit. after that, the non-absorbable fraction was anaerobically incubated with human faeces and the metabolic pathway for gallotannins, ellagitannins, flavan-3-ols ...201626868556
understanding of human metabolic pathways of different sub-classes of phenols from arbutus unedo fruit after an acute intake.arbutus unedo is a small mediterranean fruit, commonly named strawberry tree, which is a rich source of different sub-classes of phenolic compounds, the more representative being the gallic acid derivatives, including its mono and oligomeric forms esterified with quinic and shikimic acids. in addition, galloyl derivatives, particularly gallotannins, described in a. unedo, are part of a very selective phenolic group, present in a reduced number of plant-products. the aim of the present study is t ...201626960019
timing of false ring formation in pinus halepensis and arbutus unedo in southern italy: outlook from an analysis of xylogenesis and tree-ring chronologies.mediterranean tree rings are characterized by intra-annual density fluctuations (iadfs) due to partly climate-driven cambial activity. iadfs are used as structural signals to gain information on relations between environmental conditions and eco-physiological processes during xylogenesis, with intra-annual resolution. to reach an unbiased synchronization of the iadf position within tree rings and seasonal fluctuations in environmental conditions, it is necessary to know the timing of cambial act ...201627252721
secondary metabolites from the endophytic fungus talaromyces pinophilus.endophytic fungi have a great influence on plant health and growth, and are an important source of bioactive natural compounds. organic extracts obtained from the culture filtrate of an endophytic strain of talaromyces pinophilus isolated from strawberry tree (arbutus unedo) were studied. metabolomic analysis revealed the presence of three bioactive metabolites, the siderophore ferrirubin, the platelet-aggregation inhibitor herquline b and the antibiotic 3-o-methylfunicone. the latter was the ma ...201728278635
strawberry-tree honey induces growth inhibition of human colon cancer cells and increases ros generation: a comparison with manuka honey.honey is a natural product known to modulate several biological activities including cancer. the aim of the present study was to examine the phytochemical content and the antioxidant activity of strawberry tree (arbutus unedo) honey (sth) and its cytotoxic properties against human colon adenocarcinoma (hct-116) and metastatic (lovo) cell lines in comparison with manuka (leptospermum scoparium) honey (mh). several unifloral sth and mh were analyzed for their phenolic, flavonoid, amino acid and pr ...201728287469
diversity of fungal assemblages in roots of ericaceae in two mediterranean contrasting ecosystems.the plants belonging to the ericaceae family are morphologically diverse and widely distributed groups of plants. they are typically found in soil with naturally poor nutrient status. the objective of the current study was to identify cultivable mycobionts from roots of nine species of ericaceae (calluna vulgaris, erica arborea, erica australis, erica umbellate, erica scoparia, erica multiflora, arbutus unedo, vaccinium myrtillus, and vaccinium corymbosum). the sequencing approach was used to am ...201728302364
midday stomatal closure in arbutus unedo leaves in a natural macchia and under simulated habitat conditions in an environmental chamber. 198028309088
midday stomatal closure in mediterranean type sclerophylls under simulated habitat conditions in an environmental chamber : ii. effect of the complex of leaf temperature and air humidity on gas exchange of arbutus unedo and quercus ilex.shrubs of the mediterranean sclerophyllous species arbutus unedo and quercus ilex were studied under simulated habitat conditions in an environmental chamber. temperature, humidity, and light intensity were altered stepwise to simulate diurnal changes in conditions similar to those measured in an evergreen macchia in sobreda, portugal. leaves were enclosed in cuvettes which reproduced the growth chamber climate and which allowed measurement of gas exchange. increasing atmospheric stress in the f ...198128310056
diurnal variations in abscisic acid content and stomatal response to applied abscisic acid in leaves of irrigated and non-irrigated arbutus unedo plants under naturally fluctuating environmental conditions.endogenous abscisic acid content (aba) of arbutus unedo leaves growing under natural conditions in a macchia near sobreda, portugal, was very high (0.25 to 2.3 μg g(1) fresh weight). highest concentrations were found during the very early morning hours and at midday. during the late morning hours and in the late afternoon aba concentrations decreased to between one-third and one-fourth of peak values. the samples for aba content were obtained from both irrigated (ψ between-10 and-25 bar) and non ...198328310657
the responses of stomata and leaf gas exchange to vapour pressure deficits and soil water content : i. species comparisons at high soil water contents.the responses of photosynthesis, transpiration and leaf conductance to changes in vapour pressure deficit were followed in well-watered plants of the herbaceous species, helianthus annuus, helianthus nuttallii, pisum sativum and vigna unguiculata, and in the woody species having either sclerophyllous leaves, arbutus unedo, nerium oleander and pistacia vera, or mesomorphic leaves, corylus avellana, gossypium hirsutum and prunus dulcis. when the vapour pressure deficit of the air around a single l ...198428311208
the control by atmospheric factors and water stress of midday stomatal closure in arbutus unedo growing in a natural macchia.midday closure of stomata of well-watered (ψ between-10 and-25 bar) or moderately stressed (ψ between-25 and-35 bar) arbutus unedo plants occurs when midday leaf temperatures increase above 30°c and vapor pressure difference between leaf air spaces and the external air increases above approximately 30 mbar/bar. moderate water stress decreases maximum conductance and may result in greater sensitivity to high leaf temperature and vapor pressure dificit, which results in earlier closure and later r ...198228311229
effects of humidity during diurnal courses on the co2- and light-saturated rate of net co2 uptake in the sclerophyllous leaves of arbutus unedo. 198528311330
use of an analytical model to study limitations on net photosynthesis in arbutus unedo under field conditions.from field gas-exchange measurements on arbutus unedo growing in portugal, parameter values necessary to apply an analytical, physiologicallybased model of c 3 photosynthesis were obtained. the model successfully simulated measured diurnal photosynthetic responses in arbutus during periods without water stress, under both natural and co2-saturating conditions. the model was used to analyze those factors limiting primary productivity during each of the experimental days. due to a large investment ...198628311926
a fast method to detect the occurrence of nonhomogeneous distribution of stomatal aperture in heterobaric plant leaves : experiments with arbutus unedo l. during the diurnal course.pressure infiltration of water into a leaf via the stomatal pores can be used to quickly determine whether all stomata are open, or as recently described for several mesophytic and xerophytic species, whether there is a non-homogeneous distribution of stomatal opening (stomatal patchiness) on the leaf surface. information about this phenomenon is important since the commonly used algorithms for calculation of leaf conductance from water vapor exchange measurements imply homogeneously open stomat ...199028313137
water relations, gas exchange, and growth of resprouts and mature plant shoots of arbutus unedo l. and quercus ilex l.resprout and mature plant shoot growth, leaf water status and gas exchange behavior, tissue nutrient content, flowering, and production were studied for co-occurring shallow-rooted (arbutus unedo l.) and deeprooted (quercus ilex l.) mediterranean tree species at the collserola natural park in northeast spain resprouts showed higher growth rates than mature plant shoots. during fall, no differences in eco-physiological performance of leaves were found, but mobilization of carbohydrates from burls ...199428313978
genetic diversity and divergence at the arbutus unedo l. (ericaceae) westernmost distribution limit.mediterranean forests are fragile ecosystems vulnerable to recent global warming and reduction of precipitation, and a long-term negative effect is expected on vegetation with increasing drought and in areas burnt by fires. we investigated the spatial distribution of genetic variation of arbutus unedo in the western iberia peninsula, using plastid markers with conservation and provenance regions design purposes. this species is currently undergoing an intense domestication process in the region, ...201728384294
functional teas from the leaves of arbutus unedo: phenolic content, antioxidant activity, and detection of efficient radical scavengers.the phenolic content/composition and antioxidant activity of hot/cold infusion and decoction from the leaves of arbutus unedo were studied for the first time. 1,1-diphenyl-2-picrylhydrazyl (dpph(●)), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (abts(●+)), crocin-bleaching, copper-reducing, and liposome accelerated oxidation assays were used for the evaluation of the activity in vitro. in vivo, the extracts were examined for their ability to protect s. cerevisiae cells ...201728421300
effects of cold stratification and ga3 on germination of arbutus unedo seeds of three provenances.arbutus unedo is a valuable mediterranean shrub as an ornamental plant as well as fruit tree. fresh fruits of a. unedo are a good source of antioxidants, of vitamins c, e and carotenoids and also are characterized by the high content of mineral elements.028480410
development and validation of a gc-ms method for the analysis of homogentisic acid in strawberry tree (arbutus unedo l.) honey.to confirm the botanical origin of strawberry tree (arbutus unedo l.) honey, a liquid-liquid extraction followed by gc-ms method was developed for the quantitative determination of homogentisic acid (hga), the main phenolic compound in this honey. different parameters affecting extraction, such as the type and volume of extraction solvents, ph of the solution, and amount of salt, were optimized. the method showed good linearity (r2 = 0.9990) over the tested concentration range (50-500 mg/kg) and ...201728560954
the effect of silver nanoparticles size, produced using plant extract from arbutus unedo, on their antibacterial efficacy.silver nanoparticles (agnps) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. several physical and chemical methods have been reported to synthesize agnps. however, these methods are expensive and involve heavy chemical reduction agents. an alternative approach to produce agnps in a cost-effective and environmentally friendly way employs a biological pathw ...201728698511
phyto-toponyms of arbutus unedo l. and their distribution in sardinia (italy).the study shows the results of an inventory of place names connected to arbutus unedo l., a mediterranean species, widespread throughout sardinia. the main aim was to compare the past distribution of place names, referring to the strawberry tree, to the current distribution of the species on the island. in addition, we investigated the meaning and the diversity of these local place names in the various communities. the result was a collection of 432 phyto-toponyms. 248 of them were used for an a ...201728704491
inhibition of cyclic adenosine monophosphate-specific phosphodiesterase by various food plant-derived phytotherapeutic agents.background: phosphodiesterases (pdes) play a major role in the regulation of cyclic adenosine monophosphate (camp)- and cyclic guanosine monophosphate (cgmp)-mediated pathways. their inhibitors exhibit anti-inflammatory, vasodilatory and antithrombotic effects. therefore, consumption of foods with pde-inhibiting potential may possess beneficial influence on the risk of cardiovascular diseases. methods: four plant extracts (arbutus unedo, camellia sinensis, cynara scolymus, zingiber officinale) w ...201729113064
multi-elemental composition and antioxidant properties of strawberry tree (arbutus unedo l.) honey from the coastal region of croatia: risk-benefit analysis.the concentration of 23 major and trace elements, total phenolic content (tpc) and 1,1-diphenyl-2-picrylhydrazyl (dpph) radical scavenging activity were determined in nine samples of strawberry tree honey and compared to other types of unifloral honeys. the most abundant elements in strawberry tree honey were potassium, calcium, magnesium and sodium, ranging between 1276 and 2367, 95.2-154, 14.4-74.4 and 13.4-64.3mg/kg, respectively. strawberry tree honey had generally higher tpc (range: 0.314-0 ...201829173488
the effects of strawberry tree water leaf extract, arbutin and hydroquinone on haematological parameters and levels of primary dna damage in white blood cells of rats.strawberry tree (arbutus unedo l., ericaceae) leaves represent a potent source of biologically active compounds and have been used for a long to relieve symptoms of various health impairments and diseases. two major compounds related to their beneficial activities in animals and humans are arbutin and hydroquinone.201729288828
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