Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
exploring natural variation of photosynthetic, primary metabolism and growth parameters in a large panel of capsicum chinense accessions.collectively, the results presented improve upon the utility of an important genetic resource and attest to a complex genetic basis for differences in both leaf metabolism and fruit morphology between natural populations. diversity of accessions within the same species provides an alternative method to identify physiological and metabolic traits that have large effects on growth regulation, biomass and fruit production. here, we investigated physiological and metabolic traits as well as paramete ...201526007687
physiological quality and gene expression during the development of habanero pepper (capsicum chinense jacquin) seeds.phytohormones have different characteristics and functions, and they may be subject to changes in their gene expression and synthesis during seed development. in this study, we evaluated the physiological qualities of habanero peppers (capsicum chinense jacquin) during seed development and the expression of genes involved in germination. seeds were obtained from fruits harvested at different stages of development [i.e., 14, 21, 28, 35, 42, 49, 56, 63, and 70 days after anthesis (daa)]. immediate ...201526125700
biotechnological enhancement of capsaicin biosynthesis in cell suspension cultures of naga king chili (capsicum chinense jacq.).cell suspension cultures were initiated from hypocotyl derived callus to induce capsaicin biosynthesis in suspension cultures of naga king chili (capsicum chinense jacq.). efficient capsaicin production with high growth index (gi) was obtained by exposing cells to salicylic acid (sa) and calcium channel modulators in suspension cultures. the time course of capsaicin formation is related to the cell growth profile in a batch culture. cells cultivated in the standard medium (sm) initially showed l ...201626578343
a comparative study of the capsaicinoid and phenolic contents and in vitro antioxidant activities of the peppers of the genus capsicum: an application of chemometrics.this paper presents a comparison of the contents of capsaicin, dihydrocapsaicin and total phenolics as well as of the antioxidant activities of six types of peppers of the genus capsicum. the varieties were analyzed in terms of their in vitro antioxidant activity using ferric reducing antioxidant powder (frap), 1,1-diphenyl-2-picrylhydrazyl (dpph) and 2,2'-azinobis 3-ethylbenzothiazoline 6-sulfonate (abts(●+)) assays. the contents of phenolics and capsainoids as well as the antioxidant activitie ...201526604381
silencing of a germin-like protein gene (cchglp) in geminivirus-resistant pepper (capsicum chinense jacq.) bg-3821 increases susceptibility to single and mixed infections by geminiviruses phyvv and pepgmv.germin-like proteins (glps) are encoded by a family of genes found in all plants, and in terms of function, the glps are implicated in the response of plants to biotic and abiotic stresses. cchglp is a gene encoding a glp identified in a geminivirus-resistant capsicum chinense jacq accession named bg-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. to characterize the role of this glp in geminivirus resistance in the original ...201526610554
silencing of a germin-like protein gene (cchglp) in geminivirus-resistant pepper (capsicum chinense jacq.) bg-3821 increases susceptibility to single and mixed infections by geminiviruses phyvv and pepgmv.germin-like proteins (glps) are encoded by a family of genes found in all plants, and in terms of function, the glps are implicated in the response of plants to biotic and abiotic stresses. cchglp is a gene encoding a glp identified in a geminivirus-resistant capsicum chinense jacq accession named bg-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. to characterize the role of this glp in geminivirus resistance in the original ...201526703712
synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli.chilli (capsicum sp.) is one of the economically important spice and vegetable crops grown in india and suffers great losses due to the infection of begomoviruses. conventional breeding approaches have resulted in development of a few cultivars of chilli resistant to begomoviruses. a severe leaf curl disease was observed on one such resistant chilli cultivar (capsicum annuum cv. kalyanpur chanchal) grown in the experimental field of the jawaharlal nehru university, new delhi. four different vira ...201626780359
genetic diversity and molecular evolution of naga king chili inferred from internal transcribed spacer sequence of nuclear ribosomal dna.sequences of the internal transcribed spacer (its1-5.8s-its2) of nuclear ribosomal dnas were explored to study the genetic diversity and molecular evolution of naga king chili. our study indicated the occurrence of nucleotide polymorphism and haplotypic diversity in the its regions. the present study demonstrated that the variability of its1 with respect to nucleotide diversity and sequence polymorphism exceeded that of its2. sequence analysis of 5.8s gene revealed a much conserved region in all ...201626862481
increased availability of nadh in metabolically engineered baker's yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion.saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. it has a large potential to function as whole-cell biocatalyst for one-pot multistep synthesis of various organic molecules, and it may thus serve as a powerful alternative or complement to traditional organic synthetic routes for new chemical entities (nces). however, although the selectivity in many cases is high, the catalytic activity is ofte ...201626879378
water extractable phytochemicals from peppers (capsicum spp.) inhibit acetylcholinesterase and butyrylcholinesterase activities and prooxidants induced lipid peroxidation in rat brain in vitro.background. this study sought to investigate antioxidant capacity of aqueous extracts of two pepper varieties (capsicum annuum var. accuminatum (sm) and capsicum chinense (ro)) and their inhibitory effect on acetylcholinesterase and butyrylcholinesterase activities. methods. the antioxidant capacity of the peppers was evaluated by the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (abts) radical scavenging ability and ferric reducing antioxidant property. the inhibition of prooxidant ind ...201426904640
defensin γ-thionin from capsicum chinense has immunomodulatory effects on bovine mammary epithelial cells during staphylococcus aureus internalization.β-defensins are members of the antimicrobial peptide superfamily that are produced in various species from different kingdoms, including plants. plant defensins exhibit primarily antifungal activities, unlike those from animals that exhibit a broad-spectrum antimicrobial action. recently, immunomodulatory roles of mammal β-defensins have been observed to regulate inflammation and activate the immune system. similar roles for plant β-defensins remain unknown. in addition, the regulation of the im ...201626939717
pepper weevil (coleoptera: curculionidae) preferences for specific pepper cultivars, plant parts, fruit colors, fruit sizes, and timing.peppers (capsicum spp.) are an important crop in the usa, with about 32,000 ha cultivated in 2007, which resulted in $588 million in farm revenue. the pepper weevil, anthonomus eugenii cano (coleoptera: curculionidae), is the most troublesome insect pest of peppers in the southern united states. it is therefore urgent to find different vulnerabilities of pepper cultivars, fruit and plants parts, fruit colors and sizes, and timing to infestation by a. eugenii. also relevant is testing whether fru ...201626959066
digestion of chrysanthemum stunt viroid by leaf extracts of capsicum chinense indicates strong rna-digesting activity.csvd could not infect nicotiana benthamiana when the plants were pretreated with crude leaf extract of capsicum chinense 'sy-2'. c. chinense leaves were revealed to contain strong rna-digesting activity. several studies have identified active antiviral and antiviroid agents in plants. capsicum plants are known to contain antiviral agents, but the mechanism of their activity has not been determined. we aimed to elucidate the mechanism of capsicum extract's antiviroid activity. chrysanthemum stunt ...201627053224
tof-sims imaging of capsaicinoids in scotch bonnet peppers (capsicum chinense).peppers (capsicum spp.) are well known for their ability to cause an intense burning sensation when eaten. this organoleptic response is triggered by capsaicin and its analogs, collectively called capsaicinoids. in addition to the global popularity of peppers as a spice, there is a growing interest in the use of capsaicinoids to treat a variety of human ailments, including arthritis, chronic pain, digestive problems, and cancer. the cellular localization of capsaicinoid biosynthesis and accumula ...201627075215
fine mapping and identification of candidate genes for the sy-2 locus in a temperature-sensitive chili pepper (capsicum chinense).the sy - 2 temperature-sensitive gene from capsicum chinense was fine mapped to a 138.8-kb region at the distal portion of pepper chromosome 1. based on expression analyses, two putative f-box genes were identified as sy - 2 candidate genes. seychelles-2 ('sy-2') is a temperature-sensitive natural mutant of capsicum chinense, which exhibits an abnormal leaf phenotype when grown at temperatures below 24 °c. we previously showed that the sy-2 phenotype is controlled by a single recessive gene, sy- ...201627147070
variability in capsaicinoid content and scoville heat ratings of commercially grown jalapeño, habanero and bhut jolokia peppers.the variability of capsaicinoid content of three common, commercially-available hot pepper varieties, namely jalapeño, habanero and bhut jolokia, was investigated. for each variety tested, ten peppers were acquired from each of ten different suppliers resulting in 100 peppers per variety that were individually analyzed. the results showed that different pepper varieties had different distribution types. the habanero peppers showed a normal distribution; the bhut jolokia showed a skewed distribut ...201627211688
topical analgesic nanolipid vesicles formulation of capsaicinoids extract of bhut jolokia (capsicum chinense jacq): pharmacodynamic evaluation in rat models and acceptability studies in human volunteers.capsicum fruit is used for treating skeletomuscular disorders as a counterirritant analgesic around the globe. but its concentration-dependent irritation and concomitant withdrawal of therapy by the patients hampers its therapeutic usefulness. in the present study, a novel nanolipid approach based on elastic phospholipid vesicles was employed to encapsulate a semipurified extract of bhut jolokia for topical drug delivery application. the working hypothesis was that encapsulation of irritant extr ...201627306849
capsaicin synthesis requires in situ phenylalanine and valine formation in in vitro maintained placentas from capsicum chinense.capsaicinoids (cap) are nitrogenous metabolites formed from valine (val) and phenylalanine (phe) in the placentas of hot capsicum genotypes. placentas of habanero peppers can incorporate inorganic nitrogen into amino acids and have the ability to secure the availability of the required amino acids for cap biosynthesis. in order to determine the participation of the placental tissue as a supplier of these amino acids, the effects of blocking the synthesis of val and phe by using specific enzyme i ...201627338325
transcriptome analysis of capsicum chlorosis virus-induced hypersensitive resistance response in bell capsicum.capsicum chlorosis virus (cacv) is an emerging pathogen of capsicum, tomato and peanut crops in australia and south-east asia. commercial capsicum cultivars with cacv resistance are not yet available, but cacv resistance identified in capsicum chinense is being introgressed into commercial bell capsicum. however, our knowledge of the molecular mechanisms leading to the resistance response to cacv infection is limited. therefore, transcriptome and expression profiling data provide an important re ...201627398596
characterization of some indian himalayan capsicums through floral morphology and ema-based chromosome analysis.the north eastern himalayan (neh) regions of india are considered as one of the major repositories of the "capsicum annuum complex" which comprises of three cultivated species namely c. annuum, c. frutescens, and c. chinense. the interspecific delimitation within this large complex is ill-defined due to poorly developed crossing barriers and lack of discontinuous morphological characters. the present study elucidates the relationship among nine different cultivars of three capsicum species on th ...201727447699
in vitro assessment of plants growing in cuba belonging to solanaceae family against leishmania amazonensis.in this study, an in vitro antileishmanial assessment of plant extracts from 12 genera and 46 species growing in cuba belonging to solanaceae family was performed. a total of 226 extracts were screened against promastigotes of leishmania amazonensis, and cytotoxicity of active extracts [median inhibitory concentration (ic50 ) promastigotes <100 µg/ml] was determined on peritoneal macrophage from balb/c mice. extracts that showed selective index >5 were then assayed against intracellular amastigo ...201627480800
divergent evolution of multiple virus-resistance genes from a progenitor in capsicum spp.plants have evolved hundreds of nucleotide-binding and leucine-rich domain proteins (nlrs) as potential intracellular immune receptors, but the evolutionary mechanism leading to the ability to recognize specific pathogen effectors is elusive. here, we cloned pvr4 (a potyvirus resistance gene in capsicum annuum) and tsw (a tomato spotted wilt virus resistance gene in capsicum chinense) via a genome-based approach using independent segregating populations. the genes both encode typical nlrs and ar ...201727612097
esophageal rupture after ghost pepper ingestion.the ghost pepper, or "bhut jolokia," is one of the hottest chili peppers in the world. ghost peppers have a measured "heat" of > 1,000,000 scoville heat units (shu), more than twice the strength of a habanero pepper. to our knowledge, no significant adverse effects of ghost pepper ingestion have been reported.201627693067
synergistic complex from plants solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line hepg2.it had been demonstrated that sugars from various plants can act as potent agents, which induce apoptosis of cancer cells.201627756283
biochemical characterization and immunolocalization studies of a capsicum chinense jacq. protein fraction containing ding proteins and anti-microbial activity.the ding protein family consists of proteins of great biological importance due to their ability to inhibit carcinogenic cell growth. a ding peptide with mr ∼7.57 kda and pi ∼5.06 was detected in g10p1.7.57, a protein fraction from capsicum chinense jacq. seeds. amino acid sequencing of the peptide produced three smaller peptides showing identity to the ding protein family. g10p1.7.57 displayed a phosphatase activity capable of dephosphorylating different phosphorylated substrates and inhibited ...201627835848
difference in capsaicinoid biosynthesis gene expression in the pericarp reveals elevation of capsaicinoid contents in chili peppers (capsicum chinense).this research reveals that the up-regulated expression of multiple capsaicinoid biosynthetic genes in pericarp tissue leads to the elevation of total capsaicinoid content in chili pepper fruit. capsaicinoids are health-functional compounds that are produced uniquely in chili pepper fruits. a high capsaicinoid level is one of the major parameters determining the commercial quality and health-promoting properties of chili peppers. to investigate the mechanisms responsible for its high contents, we ...201727873007
comparative analysis of fruit metabolites and pungency candidate genes expression between bhut jolokia and other capsicum species.bhut jolokia, commonly known as ghost chili, a native capsicum species found in north east india was recorded as the naturally occurring hottest chili in the world by the guinness book of world records in 2006. although few studies have reported variation in pungency content of this particular species, no study till date has reported detailed expression analysis of candidate genes involved in capsaicinoids (pungency) biosynthesis pathway and other fruit metabolites. therefore, the present study ...201627936081
contents of capsaicinoids in chillies grown in denmark.we have studied 15 different cultivars of chillies (capsicum var.) grown in temperate climate denmark and determined the contents of the four major capsaicinoids: capsaicin, dihydrocapsaicin, nordihydrocapsaicin, and homocapsaicin. from these contents we have, as commonly done for chillies, calculated the so-called pungency in scoville heat units in order to compare with previous studies from other climatical zones. for three of the investigated cultivars, serrano, habanero and bih jolokia, for ...201727979294
improvement of whole-cell transamination with saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation.whole-cell biocatalysis based on metabolically active baker's yeast with engineered transamination activity can be used to generate molecules carrying a chiral amine moiety. a prerequisite is though to express efficient ω-transaminases and to reach sufficient intracellular precursor levels.201728049528
molecular cloning and functional analysis of a na(+)-insensitive k(+) transporter of capsicum chinense jacq.high-affinity k(+) (hak) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. these hak-type transporters participate in low- and high-affinity potassium (k(+)) uptake and are crucial for the maintenance of k(+) homeostasis under hostile conditions. in this study, the full-length cdna of cchak1 gene was isolated from roots of the habanero pepper (capsicum chinense). cchak1 expression was positively regulated by k(+) starvation in roots and was not inhibite ...201628083010
immunoreactivity of arcuate nucleus astrocytes in rats after intragastric administration of habanero peppers (capsicum chinese jacq.).habanero pepper fruits contain capsaicin (cap) characterised by a spicy taste. astrocytes express vanilloid receptor (trpv1), which interacts with cannabinoids including cap. only a few studies revealed that cap leads to alterations of the arcuate nucleus (arc) structures. the aim of this study was to analyse the gfap (gfap-ir) and s100β (s100β-ir) immunoreactive astrocytes of arc in adult rats after intragastric administration of habanero pepper fruits. adult, wistar rats received a peanut oil ...028092615
apocarotenoids determination in capsicum chinense jacq. cv. habanero, by supercritical fluid chromatography-triple-quadrupole/mass spectrometry.relatively few studies are available in the literature on the apocarotenoids occurrence in food which are based on liquid chromatographic separation approaches and no reports are available on the apocarotenoids separation by sfc-apci (+/-)/qqq/ms. the dual purpose of this research was to investigate the apocarotenoids presence in red habanero peppers and to develop a novel sfc-apci(+/-)/qqq/ms methodology, using a novel fused-core c30 column, for the apocarotenoids detection in a fast and effici ...201728450012
protective effect of capsaicin against methyl methanesulphonate induced toxicity in the third instar larvae of transgenic drosophila melanogaster (hsp70-lacz)bg9.capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) is the main component in hot peppers, including red chili peppers, jalapenos, and habanero, belonging to the genus capsicum. capsaicin is a potent antioxidant that interferes with free radical activities. in the present study, the possible protective effect of capsaicin was studied against methyl methanesulphonate (mms) induced toxicity in third instar larvae of transgenic drosophila melanogaster (hsp70-lacz)bg9. the third instar was allowed to ...201728527512
identification of colletotrichum isolates from capsicum chinense in amazon.chili pepper (capsicum chinense) is a great economic important culture on the state of amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. it is one of the hosts of colletotrichum genus in the north region of brazil. the aim of the study was to differentiate and to identify isolates of colletotrichum collected from c. chinense in amazon. molecular characterization, using rflp-pcr, eric-pcr and issr, was carried out initially for screening of morphologically si ...201728671253
direct online extraction and determination by supercritical fluid extraction with chromatography and mass spectrometry of targeted carotenoids from red habanero peppers (capsicum chinense jacq.).recently, supercritical fluid chromatography coupled to mass spectrometry has gained attention as a fast and useful technology applied to the carotenoids analysis. however, no reports are available in the literature on the direct online extraction and determination by supercritical fluid extraction with chromatography and mass spectrometry. the aim of this research was the development of an online method coupling supercritical fluid extraction and supercritical fluid chromatography for a detaile ...201728759173
novel approaches to extraction methods in recovery of capsaicin from habanero pepper (cnph 15.192).the objective of this study was to compare three capsaicin extraction methods: shoxlet, ultrasound-assisted extraction (uae), and shaker-assisted extraction (sae) from habanero pepper, cnph 15.192.201728808409
capsaicinoids, amino acid and fatty acid profiles in different fruit components of the world hottest naga king chilli (capsicum chinense jacq).the world hottest naga king chilli is cultivated and consumed in northeast india. capsaicinoids, amino acids and fatty acids were studied in fruit and fruit components of naga king chilli. capsaicinoid content was increased in each ripening stage and maximum level was observed at red color fruits. total protein and fat content of placenta was 19.41 and 20.36% respectively. capsaicinoids of placenta (7.35±2.241%) was higher followed by seed (3.83±1.358%) and pericarp (2.91±0.667%). similarly, ess ...201828867101
fast analysis of capsaicinoids in naga jolokia extracts (capsicum chinense) by high-performance liquid chromatography using fused core columns.a rapid high-performance liquid chromatography method with a c18 reverse-phase fused-core column has been developed for the determination and quantification of the main capsaicinoids (nornordihydrocapsaicin, nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin and homodihydrocapsaicin) present in naga jolokia peppers. a fused-core kinetex™ c18 column (50×2.1mm i.d.; 2.6μm) was used for the analysis. the chromatographic separation was obtained with a gradient method in which the mobile ...201828873562
fine mapping of the dominant potyvirus resistance gene pvr7 reveals a relationship with pvr4 in capsicum annuum.pepper mottle virus (pepmov) is the most common potyvirus infection of pepper plants and causes significant yield losses. the pvr7 gene from capsicum chinense pi159236 and the pvr4 gene from c. annuum cm334 both have been reported to confer dominant resistance to pepmov. the pvr7 locus conferring resistance to pepmov in c. annuum '9093' was previously mapped to chromosome 10. to develop a high-resolution map of the pvr7 locus in 9093, we constructed an intraspecific f2 mapping population consist ...201828945517
multiple genetic resistances in capsicum spp.this study aimed to identify capsicum genotypes with resistance to bacterial spot (bs), anthracnose and pepper yellow mosaic virus (pepymv). fifty-four genotypes of capsicum spp were evaluated. resistance reaction against bs was evaluated using three replicates, testing hypersensitivity and quantitative resistance in leaves. after evaluation, inoculated leaves were detached from the plants, being then cultivated until reproductive stage for evaluations anthracnose resistance in immature and matu ...201728973768
growth kinetics of escherichia coli o157:h7 on the epicarp of fresh vegetables and fruits.despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of escherichia coli o157:h7 on different plant substrates. this study analyzed the selective adhesion and growth of e. coli o157:h7 on chili habanero (capsicum chinense l.), cucumber (cucumis sativus), radish (raphanus sativus), tomato (lycopersicon esculentum), beet (beta vulgaris subsp. vulgaris), and onion (alliu ...201829037503
sweat bees on hot chillies: provision of pollination services by native bees in traditional slash-and-burn agriculture in the yucatán peninsula of tropical mexico.traditional tropical agriculture often entails a form of slash-and-burn land management that may adversely affect ecosystem services such as pollination, which are required for successful crop yields. the yucatán peninsula of mexico has a >4000 year history of traditional slash-and-burn agriculture, termed 'milpa'. hot 'habanero' chilli is a major pollinator-dependent crop that nowadays is often grown in monoculture within the milpa system.we studied 37 local farmers' chilli fields (sites) to ev ...201729200497
influence of water stresses on capsaicinoid production in hot pepper (capsicum chinense jacq.) cultivars with different pungency levels.although water stress reduces fruit yield, it also increases capsaicinoid accumulation in hot pepper. the aim of this study was to investigate the effect of different water regimes on capsaicinoid production in capsicum chinense jacq. having different pungency levels. four hot pepper (c. chinense) cultivars were planted with four water regimes after anthesis: daily irrigation (control; s1), every 2 days (s2), every 3 days (s3) and every 4 days (s4). the results found that akanee pirote with the ...201829287443
Displaying items 101 - 143 of 143