Publications

biocatalytic properties and structural analysis of eugenol oxidase from rhodococcus jostii rha1: a versatile oxidative biocatalyst.	eugenol oxidase (eugo) from rhodococcus jostii rha1 had previously been shown to convert only a limited set of phenolic compounds. in this study, we have explored the biocatalytic potential of this flavoprotein oxidase, resulting in a broadened substrate scope and a deeper insight into its structural properties. in addition to the oxidation of vanillyl alcohol and the hydroxylation of eugenol, eugo can efficiently catalyze the dehydrogenation of various phenolic ketones and the selective oxidati ...	2016	27123962
three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (-)-pinoresinol via kinetic resolution.	pinoresinol is a high-value plant-derived lignan with multiple health supporting effects. enantiomerically pure pinoresinol can be isolated from natural sources, but with low efficiency. most chemical and biocatalytic approaches that have been described for the synthesis of pinoresinol furnish the racemic mixture. in this study we devised a three-step biocatalytic cascade for the production of enantiomerically pure pinoresinol from the cheap compound eugenol. two consecutive oxidations of eugeno ...	2016	27160378
possible involvement of myb44-mediated stomatal regulation in systemic resistance induced by penicillium simplicissimum gp17-2 in arabidopsis.	the plant growth-promoting fungus (pgpf), penicillium simplicissimum gp17-2 (gp17-2), induces systemic resistance against pseudomonas syringae pv. tomato dc3000 (pst) in arabidopsis thaliana. the molecular mechanisms underlying induced systemic resistance (isr) by gp17-2 were investigated in the present study. microscopic observations revealed that stomatal reopening by pst was restricted by elicitation with the culture filtrate (cf) from gp17-2. a gene expression analysis of myb44, which enhanc ...	2016	27301421
okaramine g, a new okaramine congener from penicillium simplicissimum atcc 90288.	okaramine g (1), a new okaramine congener, was isolated from penicillium simplicissimum atcc 90288. the structure of 1 was determined by a spectroscopic investigation. okaramine g exhibited insecticidal activity against silkworms.	1998	27392562
extremely acidic soils are dominated by species-poor and highly specific fungal communities.	highly acidic soils (ph < 3) represent an environment which might potentially offer new biotechnologically interesting fungi. nevertheless, only little data on fungal communities in highly acidic habitats are available. here, we focused on the diversity of cultivable filamentous microfungi in highly acidic soils (ph < 3) in the czech republic. altogether, 16 soil samples were collected from four sampling sites and were processed by various approaches. in total, 54 fungal taxa were isolated and i ...	2017	27687871
potential of endophytic fungi isolated from cotton roots for biological control against verticillium wilt disease.	verticillium wilt is a soil-borne disease, and severely limits the development of cotton production. to investigate the role of endophytic fungi on verticillium wilt, cef-818 (penicillium simplicissimum), cef-714 (leptosphaeria sp.), cef-642 (talaromyces flavus.) and cef-193 (acremonium sp.) isolated from cotton roots were used to assess their effects against cotton wilt disease caused by a defoliating v. dahliae strain vd080. in the greenhouse, all treatments significantly reduced disease incid ...	2017	28107448
surface imaging of the filamentous fungus penicillium simplicissimum growing in a solid-state fermentation system.	a comparative study on the lipase-producing fungus penicillium simplissicimum, grown on a tray type solid-state fermentation (ssf) bioreactor, was performed using stereoscopy, focus z-stacking stereoscopic images, field emission scanning electron microscopy (fe-sem) and environmental scanning electron microscopy (esem) to better characterize the morphology of filamentous fungi in ssf and their distribution over the solid matrix. the imaging of live fungal samples using a stereomicroscope with fo ...	2017	28395187
a novel study based on adaptive metal tolerance behavior in fungi and sem-edx analysis.	four fungal isolates: simplicillium chinense (iso 9, accession no. kx425621), penicillium simplicissimum (iso 10, kp713758), trichoderma asperellum (iso 11, kp792512), and coriolopsis sp. (1c3, km403574) were subjected to a series of induced-tolerance training under high metal concentrations to determine if greater tolerance could be achieved from constant exposure to such conditions. adaptive tolerance assay (tolerance index, ti) and field-emission scanning electron microscopy with energy dispe ...	2017	28407540
tolerance to heavy metals in filamentous fungi isolated from contaminated mining soils in the zanjan province, iran.	in the present study the population of fungi inhabiting metal contaminated soil samples from lead-zinc mining area was investigated, and their tolerance and biosorption capacity towards cd, pb, zn and cu were evaluated. fungal species, including acremonium persicinum, penicillium simplicissimum, seimatosporium pistaciae, trichoderma harzianum, alternaria chlamydosporigena and fusarium verticillioides were isolated. fungal tolerance was determined by measuring of the "minimum inhibitory concentra ...	2017	28700958
two tyrosine residues, tyr-108 and tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase.	a number of oxidoreductases from the vao/para-cresol methylhydroxylase flavoprotein family catalyze the oxidation of para-substituted phenols. one of the best-studied is vanillyl-alcohol oxidase (vao) from the fungus penicillium simplicissimum for oxidation of phenols by vao to occur, they must first be bound in the active site of the enzyme in their phenolate anion form. the crystal structure of vao reveals that two tyrosine residues, tyr-108 and tyr-503, are positioned to facilitate this depro ...	2017	28717004
an l319f mutation in transmembrane region 3 (tm3) selectively reduces sensitivity to okaramine b of the bombyx mori l-glutamate-gated chloride channel.	okaramines produced by penicillium simplicissimum ak-40 activate l-glutamate-gated chloride channels (glucls) and thus paralyze insects. however, the okaramine binding site on insect glucls is poorly understood. sequence alignment shows that the equivalent of residue leucine319 of the okaramine b sensitive bombyx mori (b. mori) glucl is a phenylalanine in the okaramine b insensitive b. mori γ-aminobutyric acid-gated chloride channel of the same species. this residue is located in the third trans ...	2017	28825521