Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| an nad + -independent l-lactate dehydrogenase from rhizopus oryzae. | 1971 | 4334855 | |
| freezing artifacts in frozen-etched rhizopus arrhizus sporangiospores. | 1972 | 4343254 | |
| some properties of an exocellular lipase from rhizopus arrhizus. | 1974 | 4597007 | |
| total lipid and sterol components of rhizopus arrhizus: identification and metabolism. | 1973 | 4705432 | |
| ultrastructure of dormant and germinated sporangiospores of rhizopus arrhizus. | 1973 | 4707020 | |
| roles of tryptophan residues on the rhizopus delemar lipase activity: chemical modification in a water-olive oil emulsion. | 1973 | 4778940 | |
| microbiological hydroxylation of steroids. ix. hydroxylation of diketones and keto-alcohols derived from 5 alpha-androstane with the fungi rhizopus arrhizus and rhizopus circinnans. steroidal 18- and 19-proton magnetic resonance signals. | 1973 | 4796662 | |
| studies on the structure and mechanism of action of glycoside hydrolases. i. purification and study of some factors affecting the activity of rhizopus arrhizus (1 leads to 3)-beta-d-glucanase. | 1974 | 4842010 | |
| influence of oxygen substituents at the c-11, -17, -20, and -21 positions on the extent of 6beta-hydroxylation of steroids by the mold rhizopus arrhizus. | 1974 | 4845230 | |
| ultrastructural details in germinating sporangiospores of rhizopus stolonifer and rhizopus arrhizus. | electron microscope examination of sporangiospore sections from rhizopus stolonifer (ehrenb. ex fr.) lind. and r. arrhizus fischer revealed details on intracellular organization not previously reported. aldehyde fixation followed by chromeosmium postfixation permitted clear depiction of ribosomes hitherto unrevealed in these cells. mitochondria were diversiform. spore wall structures in the two species were generally similar, but outer contours differed sufficiently to permit easy species identi ... | 1968 | 4876136 |
| the exocellular lipase from rhizopus arrhizus. | 1971 | 4997866 | |
| effect of dimethyl sulphoxide on growth, sporulation and spore germination of rhizopus arrhizus fischer. | 1972 | 5024636 | |
| total, polar and neutral lipids of rhizopus arrhizus fischer. | 1972 | 5038496 | |
| further studies on the exocellular lipase of rhizopus arrhizus. | 1972 | 5046808 | |
| tryptophan stimulation of growth and sporulation of rhizopus arrhizus fischer. | 1972 | 5052891 | |
| characteristics of an in vitro phenylalanine incorporating system from rhizopus arrhizus. | 1972 | 5061184 | |
| changes in esters of fatty acids of rhizopus arrhizus during germination and growth. | natural esters of fatty acids of rhizopus arrhizus fischer increased during the first 48 hr of growth. during the 48- to 72-hr period, there was a shift in concentration from the methyl esters to the ethyl esters of fatty acids. | 1972 | 5079078 |
| [i. transformation of scillarenin by rhizopus arrhizus. ii. structure clarification of the scillarenin transformation products of rhizopus arrhizus]. | 1971 | 5154908 | |
| [purification and properties of the beta-(1-3)-glucanase of rhizopus arrhizus]. | 1971 | 5159957 | |
| stereochemistry of microbiological hydroxylation. 3. hydroxylation of 3-benzoyl-3-azabicyclo[3.3.1]nonane with rhizopus arrhizus. novel chromic acid oxidations of substrate and product. | 1969 | 5357524 | |
| some properties of a lipase from rhizopus arrhizus. separation of a glycopeptide bound to the enzyme. | 1969 | 5363985 | |
| characterization of ribosomes from the phycomycetes, rhizopus arrhizus and pythium debaryanum. | 1970 | 5413474 | |
| hemolytic activity of rhizopus nigricans and rhizopus arrhizus. | 1970 | 5466029 | |
| lipids of rhizopus arrhizus fischer. | the lipids of rhizopus arrhizus fischer mycelia and sporangiospores were extracted, isolated, and separated by thin-layer, liquid, and gas chromatography. structural confirmations of the compounds were made by a gas chromatographmass spectrometer combination. the n-heptane fraction contained squalene (1%) as a major hydrocarbon constituent. other major lipid classes detected were free fatty acids, naturally occurring methyl esters of fatty acids, triglycerides, sterols, and polar lipids. the pol ... | 1970 | 5474875 |
| [some morphological and physiological properties of rhizopus delemar]. | 1970 | 5497282 | |
| pharmacological and toxicological studies on a starch diastatic enzyme "new sumyzyme" produced by rhizopus delemar var. multiplici-sporus. | 1970 | 5535754 | |
| [lipase of rhizopus arrhizus. isolation and properties of the pure enzyme]. | 1968 | 5716418 | |
| studies on carbohydrate-metabolizing enzymes. 11. the hydrolysis of lichenin by enzyme preparations from malted barley and rhizopus arrhizus. | 1964 | 5833367 | |
| role of proline and glutamic acid metabolism in spore germination of rhizopus arrhizus. | 1966 | 5901876 | |
| further studies on germination of sporangiospores of rhizopus arrhizus. | 1966 | 5912591 | |
| [lipase of rhizopus aerhizus. obtaining, purification and properties of the lipase of rhizopus arrhizus var. delemar]. | 1966 | 5958554 | |
| a kinetic method for the determination of number-average molecular weight of linear high polymer by using an exo-enzyme. number-average molecular weights of various fractions of amylose determined by using gluc-amylase of rhizopus delemar. | 1966 | 5970083 | |
| antifungal activity of isothiocyanates and related compounds. i. naturally occurring isothiocyanates and their analogues. | data are presented concerning the antifungal activity of 11 natural isothiocyanates and 27 synthetized analogues in aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as in 13 additional saprophytic and parasitic fungi. a remarkable antifungal activity was observed in some analogues of benzylisothiocyanate and beta-phenylethylisothiocyanate. the latter-mentioned compounds have not been described previously. in the group of benzylisothiocyanates, a correlation, which was inver ... | 1967 | 6049294 |
| antifungal activity of isothiocyanates and related compounds. ii. mononuclear aromatic isothiocyanates. | antifungal activity on aspergillus niger, penicillium cyclopium, and rhizopus oryzae, as well as on additional saprophytic and parasitic fungi, was determined in 57 substituted derivatives of phenylisothiocyanate. most of the investigated compounds displayed rather equal activity against the three mentioned fungi, in contradistinction to the analogues of natural benzyl- and beta-phenylethylisothiocyanate with their characteristic low activity against r. oryzae. differences occurred in the type o ... | 1967 | 6049295 |
| [on rhizopus arrhizus lipase. i. positional specificity]. | 1967 | 6055266 | |
| purification of a lipase from rhizopus arrhizus. identification of two active forms of the enzyme. | 1967 | 6078132 | |
| utilization of diacylglycerol in phospholipid bilayers by pig brain diacylglycerol kinase and rhizopus arrhizus lipase. | diacylglycerol kinase purified from pig brain cytosol could use sonication-dispersed diacylglycerol in the presence of its activator, phosphatidylcholine vesicles. however, the kinase failed to significantly use diacylglycerol cosonicated with phosphatidylcholine. similarly, the kinase could not use diacylglycerol generated in microsomes by the back reaction of diacylglycerol choline phosphotransferase, though phospholipase c treatment of microsomes yielded effective substrate for the kinase. in ... | 1984 | 6088535 |
| [further examinations of the influence of somatostatin on triglyceride absorption (author's transl)]. | 7 healthy subjects received an oral fat load containing 100 g neutral fat on two test days. the test meal was followed by an infusion of somatostatin (500 micrograms/h) over three hours. on the first test day 150,000 e of an active fungal lipase (extracted from rhizopus arrhizus) were given with the fat meal. on the second day placebo capsules were administered instead of lipase. no postprandial increase of serum triglyceride levels could be found in both examinations. these results suggest, tha ... | 1980 | 6104384 |
| antigenic characterization of some potentially pathogenic mucoraceous fungi. | the antigenic profiles of 10 mucoraceous fungi--absidia corymbifera, mortierella wolfii, mucor miehei, m. pusillus, m. racemosus, rhizopus arrhizus, r. microsporus, r. oryzae, r. rhizopodiformis, r. stolonifer,--candida albicans and aspergillus fumigatus were compared by crossed immunoelectrophoresis (xie). antigen-rich material was obtained from homogenized hyphae (or yeasts in the case of c. albicans), and antisera by multiple subcutaneous innoculation of rabbits with macerated but viable hyph ... | 1982 | 6182620 |
| hydroxamate siderophore production by opportunistic and systemic fungal pathogens. | it has been suggested that siderophores may function as virulence factors. there have been few studies on production of siderophores by opportunistic and pathogenic fungi. we examined siderophore production by absidia corymbifera, aspergillus niger, rhizopus arrhizus, rhizopus oryzae, blastomyces dermatitidis, histoplasma capsulatum, sporothrix schenickii, candida albicans, and trichophyton mentagrophytes. fungi were cultured at 37 and 27 degrees c in a chemically defined low-iron media (0.2 mic ... | 1983 | 6221998 |
| selective effects of two systemic fungicides on soil fungi. | bas 317 00f was not toxic to the total count of fungi after 2 days but was regularly significantly toxic at the three doses after 5, 20 and 40 days and toxic at the low and the high doses after 80 days. in the agar medium, it was toxic to the counts of total fungi. aspergillus, a. terreus, rhizopus oryzae and mucor racemosus at the high dose. only the mycelial growth of trichoderma viride which was significantly inhibited by the three doses when this fungicide was added to the liquid medium. pol ... | 1982 | 6290890 |
| damage to aspergillus fumigatus and rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. | our previous studies established that human neutrophils could damage and probably kill hyphae of aspergillus fumigatus and rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. these studies were extended, again quantitating hyphal damage by reduction in uptake of (14)c-labeled uracil or glutamine. neither a. fumigatus nor r. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative ... | 1982 | 6292103 |
| in vivo bronchoalveolar macrophage defense against rhizopus oryzae and aspergillus fumigatus. | the ability of bronchoalveolar macrophages from normal, diabetic, and cortisone-treated mice to inhibit spore germination and kill fungal spores in vivo was investigated. the data indicated that the normal host controls different fungal infections in the lungs by different mechanisms. prevention of mucormycosis required inhibition of fungal spore germination by alveolar macrophages. in contrast, pulmonary defense against aspergillosis depended on early killing of conidia by alveolar macrophages ... | 1984 | 6387001 |
| immunodiffusion and complement fixation assays with sera from mucormycotic-infected mice. | results using homogenate antigens of rhizomucor pusillus against immunized rabbit sera, mouse ascites fluid and sera from mucormycotic-infected mice, detected by immunodiffusion and complement fixation assays are presented. using immunodiffusion techniques, common antigens and unique antigens from rh. pusillus, rhizopus oryzae and absidia corymbifera were observed. complement fixation titers of greater than 256 and 128 were obtained in immunized rabbit sera and mouse ascites fluid, respectively. ... | 1983 | 6422303 |
| inhibition of pancreatic and microbial lipases by proteins. | we have compared the effect of several proteins, including melittin, beta-lactoglobulin a, serum albumin, ovalbumin and myoglobin, on the hydrolysis of tributyrin and triolein by lipases from various origins. all proteins tested inactivate pancreatic lipase in absence of colipase and bile salt. inhibition is not significantly reversed by colipase in absence of bile salt except in systems containing tributyrin and melittin or triolein and beta-lactoglobulin a. in all other cases, activation of pa ... | 1984 | 6477949 |
| [growth and biotin synthesis of sporobolomyces pararoseus and rhizopus delemar under different substrate ratios]. | biotin synthesis by the yeast sporobolomyces pararoseus and the microscopic fungus rhizopus delemar was studied in batch cultures at different substrate ratios in the medium. the overall (exocellular + endocellular) biotin content in the yeast was 4.5-6.0 micrograms per 1 g of biomass on different media and did not depend on the growth phase. the content of biotin in the fungus was 7.5-9.0 micrograms per 1 g of biomass and depended neither on the growth phase nor on the quantitative ratio betwee ... | 1984 | 6541287 |
| [desthiobiotin transformation to biotin by the fungus rhizopus delemar]. | the object of this work was to study the transformation of desthiobiotin (dtb), a biotin precursor, to biotin by rhizopus delemar which can accumulate rather large quantities of biotin in the medium. both the growing and resting culture transform dtb at a high rate. biotin excretion by the resting culture rises by 100% when 25 micrograms/ml of dtb are added to the medium. the content of biotin within the cells remains unchanged. l-methionine, sodium sulfite and sodium sulfate are most effective ... | 1983 | 6664312 |
| cerebral mucormycosis in diabetic mice after intrasinus challenge. | rhinocerebral involvement is the most common form of mucormycosis in uncontrolled diabetic patients. streptozotocin-induced diabetic and normal mice were challenged by intrasinus inoculation of rhizopus oryzae to determine their susceptibility to infection by this route. ninety percent of diabetic mice and no normal mice died after inoculation with 10(6) spores. intrasinus inoculations caused minimal direct trauma and low levels of brain inocula, but progressive cerebral infections were induced ... | 1984 | 6706405 |
| use of flow cytometry in industrial microbiology for strain improvement programs. | a flow cytometry (fcm) system was chosen to analyze and sort microbiological samples, e.g., bacteria, bacterial spores, yeasts, and fungal spores, without major changes in the commercially available state. the system was further improved by addition of a stepping motor-driven scanning table that accepts standard petri dishes or microtiter plates. the electronics of the sorting system were changed to enable the sorter to deliver only one particle at a time, working in a "handshake" mode with the ... | 1984 | 6714026 |
| antimicrobial effects of esters and amides of 3-(5-nitro-2-furyl)acrylic acid. | the effect of 18 newly synthesized esters and amides of 3-(5-nitro-2-furyl)acrylic acid on bacteria (escherichia coli, staphylococcus aureus), yeasts (saccharomyces cerevisiae, candida albicans), molds (aspergillus niger, penicillium cyclopium, rhizopus oryzae) and algae (chlorella pyrenoidosa, euglena gracilis, scenedesmus obliquus) was investigated. the mic values revealed antimycotic, antialgal and antibacterial activity of the studied derivatives. the antimycotic activity was found to decrea ... | 1984 | 6714854 |
| specific susceptibility to mucormycosis in murine diabetes and bronchoalveolar macrophage defense against rhizopus. | to assess the influence of diabetes mellitus in predisposing to pulmonary mucormycosis, a murine model of streptozotocin-induced diabetes was used. intranasal inoculation of rhizopus oryzae into diabetic mice resulted in mucormycotic infection with histopathology resembling pulmonary mucormycosis observed in humans. there was no mortality nor infection in inoculated normal mice. diabetic mice had fatal infections caused by r. oryzae but significantly reduced mortality following inoculation with ... | 1984 | 6736246 |
| inducibility of cytochrome p-450 and of nadph-cytochrome c reductase in progesterone treated filamentous fungi rhizopus nigricans and rhizopus arrhizus. | 1981 | 6785529 | |
| a mechanism of susceptibility to mucormycosis in diabetic ketoacidosis: transferrin and iron availability. | the defect in host defense that makes the diabetic ketoacidotic (dka) patient susceptible to mucormycosis has not been identified. sera from 10 dka patients and three normal volunteers were tested for their capacity to support the in vitro growth of a common etiologic agent of mucormycosis, rhizopus oryzae. after equilibration with room air none of the normal or dka sera, each of which was now extremely alkaline, supported growth of r. oryzae. when the sera were placed in a co2 atmosphere that p ... | 1982 | 6816646 |
| generation of chemotactic factors by rhizopus oryzae in the presence and absence of serum: relationship to hyphal damage mediated by human neutrophils and effects of hyperglycemia and ketoacidosis. | as our previous studies had shown that human neutrophils could kill rhizopus oryzae hyphae in vitro, interactions of these hyphae with neutrophils and serum were further explored. heated or fresh normal human sera suppressed hyphal metabolic activity as determined by [14c]uracil uptake, but severe ketoacidosis (8 x 10(-3) m beta-hydroxybutyric acid plus 2 x 10(-3) m acetoacetic acid at ph 7.0) negated this effect. hyperglycemia (500 mg/dl) and severe ketoacidosis did not affect damage to hyphae ... | 1982 | 6818145 |
| ultracytochemical observations on intracytoplasmic lipids by enzymic digestive methods: histochemical trials. | the penetration of rhizopus arrhizus lipase into ethionine fatty livers was examined histochemically. a free-floating, 30 microns thick vibratome section of the livers perfused with aldehydes was shown to be suitable for ultracytochemical analysis by the lipase digestive method. accessibility of phospholipids in the outer membrane of erythrocytes to naja naja phospholipase a2 was observed under a light microscope, and intact red cells were used for the ultracytochemical analysis by the phospholi ... | 1983 | 6827071 |
| the comparative virulence of thermotolerant mucorales species in mice. | the comparative virulence of thermotolerant mucorales was determined for cortisone-treated and untreated swiss mice by intravenous administration of spores. the measure of virulence was based on an ld50 value, calculated after the 30-day observation period. of the known etiological agents of mucormycosis, mucor meihei, m. pusillus, rhizopus arrhizus, r. chinensis, r. cohnii, r. microsporus, r. oryzae, r. rhizopodiformis and cunninghamella elegans were able to produce fatal infections in mice; wh ... | 1983 | 6877338 |
| [optimization of the medium for biotin biosynthesis by a culture of rhizopus delemar]. | the effect of nitrogen and carbon sources on the biotin biosynthesis by the fungal culture rhizopus delemar 1428 has been investigated. peptone and soy-bean flour have been shown to stimulate biotin synthesis. with the aid of mathematical planning methods the optimal composition of the nutrient medium and optimal concentrations of its components have been selected. the cultivation of rh. delemar 1428 on the thus developed medium leads to the accumulation of 600 micrograms/1 biotin in the culture ... | 1983 | 6878207 |
| fungistatic mechanism of human transferrin for rhizopus oryzae and trichophyton mentagrophytes: alternative to simple iron deprivation. | human serum, human transferrin (tf), and the iron chelator 1,10-phenanthroline (op) produce iron-reversible fungistatic activity which has been attributed to simple iron deprivation. in this study, the influence of the size of the inoculum on the inhibitory activity of serum, tf, and op prepared with the same iron-binding capacity (2.5 micrograms/ml) for rhizopus oryzae and trichophyton mentagrophytes was examined. inhibition was monitored in liquid microcultures maintained at 37 degrees c and p ... | 1983 | 6885162 |
| increase in the activity of rhizopus delemar lipase on water-soluble esters by its binding with phosphatidylcholine. | rhizopus (rh.) delemar (atcc 34612) c-lipase was found to exhibit a slight activity towards water-soluble esters. the hydrolytic reaction of this lipase on alpha-naphthyl acetate was competitively inhibited by the presence of olive oil or tween 80. this finding showed that both substrates, insoluble triglyceride and water-soluble ester, were hydrolyzed at the same site on the enzyme. the activities on water-soluble esters (alpha-naphthyl acetate, beta-naphthyl acetate, methyl acetylsalicylate an ... | 1983 | 6885742 |
| nosocomical rhizopus infection (zygomycosis) in children. | three children with the rare occurrence of zygomycosis are descibed: two had involvement of a solitary lesion of gangrenous cellulitis on the buttocks, and th third was a neonate with gastric performation and a gangrenous appendicitis. all three patients were compromised hosts (two with leukemia and one a premature infant with respiratory distress syndrome). all three patients appeared to have acquired the same organism. rhizopus oryzae, from the same fomites, elastic bondages (elastoplast). the ... | 1980 | 6928943 |
| some studies on the composition and surface properties of oil bodies from the seed cotyledons of safflower (carthamus tinctorius) and linseed (linum ustatissimum). | 1. the average oil-body diameter in intact cells of developing linseed (linum usitatissimum) and safflower (carthamus tinctorius) cotyledons was similar (about 1.4 micrometer), and there was little change in size after oil bodies were isolated and repeatedly washed. 2. the glycerolipid composition of washed oil bodies from both developing and mature cotyledons of the two species was similar; oil bodies from ten different batches of cotyledons contained 4.3 +/- 0.16 mumol of 3-sn-phosphatidylchol ... | 1980 | 7008782 |
| antimycotic activity of berberine sulphate: an alkaloid from an indian medicinal herb. | berberine sulphate in concentrations of 10-25 mg ml -1 inhibited the growth of 11/13 fungi, viz. alternaria, aspergillus flavus, asp. fumigatus, candida albicans, curvularia, drechslera, fusarium, mucor, penicillium, rhizopus oryzae and scopulariopsis. concentrations of 50 mg ml -1 could check the growth of syncephalastrum as well but asp. niger remained unaffected. | 1982 | 7038929 |
| is platelet-activating factor (paf-acether) synthesis by murine peritoneal cells (pc) a two-step process? | paf-acether (1-o-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) is released from several cell sources simultaneously with an inactive non-acylated compound (1-o-alkyl-glyceryl-3-phosphorylcholine) (lyso-paf-acether). formation of the latter probably results from the activation of a phospholipase a2 (pla2). indeed, the pla2 inhibitors, bromophenacyl bromide (bpb), mepacrine, 874cb (100 microm), and edta (5 mm), blocked the zymosan-induced release of paf-acether from pc. edta and bpb also markedl ... | 1981 | 7053072 |
| fungistatic capacity of sera from guinea pigs injected with various iron solutions: differences between trichophyton mentagrophytes and rhizopus oryzae. | the fungistatic capacity and serum ion levels (si) of guinea pigs given subcutaneous injections of various iron solutions were examined. the administration of 2.0 ml of 0.1 m ferric ammonium sulfate, ferric sulfate, or ferric chloride subcutaneously had no significant effect on the si 3 h after administration, whereas ferric ammonium citrate, ferric citrate, or ferrous sulfate elevated the si to 50 to 140 times that necessary to saturate the unbound transferrin in normal sera. the sera from 11 o ... | 1982 | 7054128 |
| stability and inhibition of aspergillus and rhizopus lipases. | a lipase (triacylglyceral aclhydrolase, ec 3.1.1.3) secreted by aspergillus contains two chains each of molecular weight 25000, with 12 mannose, two galactose and two n-acetylglucosamine residues per chain. the average hydrophobicity is close to the mean fov globular proteins. thermal denaturation was biphasic when followed by circular dichroism but the stability is not unusual for globular proteins, nor does partial removal of the carbohydrate affect it. (delta g=46kj). refolding did not occur. ... | 1982 | 7055576 |
| lipase-induced alterations of fatty acid synthesis by subcellular fractions from germinating pea (pisum sativum l.). | 1. the effect of exogenous lipases on fatty acid synthesis from [14c]malonyl-coa by the microsomal and soluble fractions from germinating peas was studied. 2. addition of phospholipase a2 or the lipase from rhizopus arrhizus had no effect on total fatty acid synthesis by the soluble fraction but caused severe inhibition of that by the microsomal fraction. 3. the addition of enzymes with phospholipase activity particularly inhibited the microsomal stearate elongase. 4. control studies indicated t ... | 1982 | 7115342 |
| monocyte-mediated damage to rhizopus oryzae hyphae in vitro. | clinicopathological correlations from human cases and experimental animal studies suggest that neutrophils are critical components of the host response to mucormycosis but that other cellular defense mechanisms appear to be important as well. since our previous studies demonstrated that rhizopus oryzae hyphae which are too large to be ingested completely can be damaged and probably killed by human neutrophils, we studied the antihyphal activity of human monocytes. as with neutrophils, light and ... | 1982 | 7141693 |
| [biotin formation by the fungus rhizopus delemar]. | the capability of 26 collection micromycete strains to synthesize biotin and transform destiobiotin was investigated. destiobiotin stimulated the biotin formation by the strains of the aspergillus genus (except a. sclerotiorum synthesizing vitamin traces), the fusarium genus, the rhizopus genus (except rh. nigricans), as well as thighemella hyalospora and sporotrichum sulfurescens. among the strains tested rhizopus delemar 1428 synthesized maximum quantities of biotin (over 300 micrograms/1). th ... | 1982 | 7145874 |
| preparation of thioester substrates and development of continuous spectrophotometric assays for phospholipase a1 and monoacylglycerol lipase. | current assays of phospholipase a1 (ec 3.1.1.32) and monoacylglycerol lipase (ec 3.1.1.23) activities in tissues are discontinuous, laborious, and expensive. some spectrophotometric substrates were synthesized to alleviate this problem. thioester analogs of phosphatidylcholine and phosphatidylethanolamine. rac-1,2-s,o-didecanoyl-3-phosphocholine-1-mercapto-2,3-propanediol and rac-1,2-s,o-didecanoyl-3-phosphoethanolamine-1-mercapto-2,3-propanediol, were synthesized from the diacylglycerol analog, ... | 1981 | 7240974 |
| [immobilized lipase from rhizopus oryzae 14-14]. | properties of lipase immobilized from rhizopus oryzae 14-14 immobilized on inorganic supports-aluminium oxide and silica gel-were investigated. aluminium oxide immobilized lipase retained 23% of its initial activity when tributyrin was used as substrate. ph optimum of action of immobilized lipase was at 8.75, i.e. it was shifted toward the more alkaline area as compared to ph optimum of the non-immobilized enzyme which was in the area of 7.0-7.8. the temperature optimum of action of the immobili ... | 1981 | 7279881 |
| fatty acid elongation by a particulate fraction from germinating pea. | the synthesis of fatty acids from [14c]malonyl-coa was studied with a high-speed particulate fraction from germinating pea (pisum sativum). the variety used (feltham first) produced mainly saturated fatty acids with palmitate (30--40%) and stearate (40--60%) predominating. several palmitate-containing lipids stimulated overall synthesis and, in addition, increased the percentage of label in stearate. the production of stearate was severely inhibited by preincubation of the microsomal fraction wi ... | 1980 | 7283974 |
| reversibility of the modification of rhizopus delemar lipases by phosphatidylcholine. | rhizopus (rh.) delemar (atcc 34612) lipase is modified by its binding with phosphatidylcholine (pc); such binding enhances the lipoprotein lipase (lpl) activity, shifts the isoelectric point (pi) to the acidic side and decreases its alpha-helical content ((1980) j. biochem. 88, 533-538). the results of density gradient ultracentrifugation proved that pc binding to lipase molecule was depleted by the treatment of pc-bound lipase with 0.3% triton x-100 and 0.1 m nacl. by this treatment, lpl activi ... | 1981 | 7287646 |
| susceptibility of zoopathogenic fungi to phytoalexins. | phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. they are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. employing a broth dilution technique, we have found the zoopathogens petriellidium boydii, aspergillus flavus, a. fumigatus, candida albicans, coccidioides immitis, crypto ... | 1980 | 7387136 |
| microbiological transformation of 1,2-diphenyl-3,5 dioxo-4-n-butyl pyrazolidine (phenylbutazone). | microbiological conversion of phenylbutazone with rhizopus arrhizus fischer gave 4-hydroxy phenylbutazone in excellent yield. spectral studies, mass spectrometry, in particular, were made of the obtained metabolite; other experiments performed with the intact microorganism or with the cell-free system, as well as studies with the well-known metabolic inhibitor, skf 525 a, indicated that the obtained 4-hydroxy phenylbutazone was not an artefact. similar experiments were performed on oxyphenbutazo ... | 1980 | 7389746 |
| fungi from interior organs of free-living small mammals in czechoslovakia and yugoslavia. | a total of 308 fungi was isolated from interior organs (lungs, spleen, liver) of 529 small mammals belonging to 21 species, 7 families and 3 orders (insectivora, chiroptera, rodentia), some of these being potentially pathogenic to vertebrates (e.g. aspergillus flavus, a. fumigatus, geotrichum candidum, mucor pusillus, rhizopus arrhizus). in one vole (microtus arvalis) captured in south moravia, adiaspiromycosis (emmonsia crescens) was demonstrated. comparison of mycoflora of hair and that of int ... | 1980 | 7419129 |
| modification of rhizopus delemar lipase by its binding with phospholipids. | rhizopus delemar (atcc 34612) lipase was modified by phospholipid (pl)-treatment so as to enhance its activity on lipoprotein. in order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove pl from the pl-treated lipase solution which included pl nonessential to enhancement of lipoprotein lipase (lpl) activity. it was found that treatment with a mixture of isopropyl ether: n-butanol (3 : 1) was suitable for this purpose because of the sta ... | 1980 | 7419510 |
| the fumr gene encoding fumarase in the filamentous fungus rhizopus oryzae: cloning, structure and expression. | the filamentous fungus rhizopus oryzae (ro) is known for its ability to overproduce and accumulate high levels of fumaric acid (fa) under stress conditions. in order to study the molecular mechanisms involved in the increased biosynthesis of fa, the gene (designated fumr) encoding ro fumarase was cloned and analysed for its structure and expression. nucleotide (nt) sequence and comparison of the fumr product with fumarases from various sources established that fumr contains nine introns and enco ... | 1995 | 7557464 |
| acute rhinocerebral mucormycosis caused by rhizopus arrhizus from sri lanka. | rhinocerebral mucormycosis is a rare fungus infection reported mainly from the united states of america and europe. the disease is caused by zygomycete fungi, most often by a rhizopus species. diagnosis is often made post-mortem and in many instances culture identification of the fungus responsible has not been performed. a case of culturally proven rhinocerebral mucormycosis is described for the first time in sri lanka. the patient was a 56-year-old male who had been treated for diabetes mellit ... | 1995 | 7563267 |
| contribution of selected fungi to the reduction of cyanogen levels during solid substrate fermentation of cassava. | the effect of six individual strains of the dominant microflora in solid substrate fermenting cassava on cyanogen levels was examined. six out of eight batches of disinfected cassava root pieces were incubated for 72 h after inoculation with either of the fungi geotrichum candidum, mucor racemosus, neurospora sitophila, rhizopus oryzae and rhizopus stolonifer, or a bacillus sp., isolated from on-farm fermented cassava flours from uganda. one non-inoculated batch was incubated as a reference. lev ... | 1995 | 7577362 |
| cloning of the rhizopus niveus pyr4 gene and its use for the transformation of rhizopus delemar. | we have cloned a pyr4 gene encoding orotidine-5'-monophosphate decarboxylase of the filamentous fungus rhizopus niveus. the pyr4 gene of r. nivens has an open reading frame composed of 265 amino-acid residues and has two putative introns. we have also isolated a pyr4 mutant of rhizopus delemar from 5-fluoroorotic acid-resistant mutants and transformed it with the pyr4 gene of r. niveus as a selectable marker. introduced dna was integrated into the chromosome in a multiple tandem array. the mitot ... | 1995 | 7586035 |
| effect of increasing inoculum sizes of pathogenic filamentous fungi on mics of antifungal agents by broth microdilution method. | inoculum size is a critical variable in development of methods for antifungal susceptibility testing for filamentous fungi. in order to investigate the influence of different inoculum sizes on mics of amphotericin b, 5-fluorocytosine, itraconazole, and miconazole, 32 clinical isolates (8 aspergillus fumigatus, 8 aspergillus flavus, 5 rhizopus arrhizus, 8 pseudallescheria boydii, and 3 fusarium solani isolates) were studied by the broth microdilution method. four inoculum sizes were studied: 1 x ... | 1995 | 7615745 |
| reducing cassava toxicity by heap-fermentation in uganda. | processing of cassava roots by the alur tribe in uganda includes a stage of solid substrate fermentation in heaps. changes in cyanogen levels during the process, microflora involved, and protein levels, amino acid patterns and mycotoxin contamination of the final products were studied. processing was monitored at six rural households and repeated at laboratory site, comparing it to sun-drying. flour samples from rural households were analysed for residual cyanogens, mutagenicity, cytotoxicity an ... | 1995 | 7621084 |
| inversion of lipase stereospecificity for fluorogenic alkyldiacyl glycerols. effect of substrate solubilization. | we synthesized enantiomeric 1-o-alkyl-2,3-diacyl-sn-glycerol and 3-o-alkyl-1,2-diacyl-sn-glycerol containing pyrene as a fluorescent reporter and the trinitrophenylamino residue as a fluorescence quencher; both reporter groups were covalently bound to the omega end of the acyl chains at positions sn-2 and sn-3(1), respectively. the fluorescence of the intact substrate molecules was very low. chemical or enzymic release of the fatty acyl chains lead to fluorescence dequenching. the rate of lipoly ... | 1995 | 7628484 |
| pyoderma gangraenosum caused by rhizopus arrhizus. | a rare case of pyoderma gangraenosum caused by rhizopus arrhizus is reported. the patient, a 50-year-old male farmer, was admitted to hospital complaining of gangrening and festering of the right upper arm with severe pain for nearly 2 months. a lesion was found on the inside of right upper arm. the central skin part of the lesion became black dry gangrenous, the periphery was deeply ulcerated with yellow-green pus. the necrotic crust, biceps, triceps and vessels inherited a histopathologically ... | 1995 | 7637685 |
| the dependence of the lipolytic activity of rhizopus arrhizus lipase on surfactant concentration in aerosol-ot/isooctane reverse micelles and its relationship to enzyme structure. | aerosol ot, bis(2-ethylhexyl)sodium sulfosuccinate/isooctane reverse micelles were used to investigate the dependence of the lipolytic activity of rhizopus arrhizus lipase on surfactant concentration. kinetic constants for the lipolytic reaction were measured in parallel with structural studies using protein fluorescence and circular dichroism (cd) spectroscopy. km values remained constant throughout the range of aot concentrations studied. the kcat values decreased with increasing surfactant co ... | 1993 | 7678504 |
| comparative and collaborative evaluation of standardization of antifungal susceptibility testing for filamentous fungi. | the purpose of the study was to evaluate the interlaboratory agreement of broth dilution susceptibility methods for five species of conidium-forming (size range, 2 to 7 microns) filamentous fungi. the methods used included both macro- and microdilution methods that were adaptations of the proposed reference method of the national committee for clinical laboratory standards for yeasts (m27-p). the mics of amphotericin b, fluconazole, itraconazole, miconazole, and ketoconazole were determined in s ... | 1995 | 7726488 |
| molecular and enzymatic properties of an aspartic proteinase from rhizopus hangchow. | an aspartic proteinase, rhizopuspepsin (ec 3.4.23.21), from rhizopus hangchow was purified. the m(r) and isoelectric point were determined as ca 37,000 and 4.5, respectively. the first 19 amino acids in the n-terminal region were sgsgvvpmtdyeydieyyg. the contents of the alpha-helix, beta-structure and random coil were calculated to be ca 7.5, 88.9 and 2.7%, respectively. the enzyme can activate trypsinogen at ph 3.0. the activity was completely inactivated by pepstatin a. the specificity and mod ... | 1995 | 7766059 |
| stereoselectivity of microbial lipases. the substitution at position sn-2 of triacylglycerol analogs influences the stereoselectivity of different microbial lipases. | in the present study, the stereoselectivity of purified lipases from candida rugosa, chromobacterium viscosum, pseudomonas species and rhizopus arrhizus towards triacylglycerols in comparison to various structural analogs were investigated. different triacylglycerol analogs with distinct polarities at position sn-2 of the glycerol backbone (1,3-diacyl-2-x-glycerol, where 2-x = 2-acyloxy, 2-alkyloxy, 2-deoxy-2-alkyl, or 2-deoxy-2-phenyl) were synthesized. substrate hydrophobicity and steric requi ... | 1995 | 7851405 |
| cloning and nucleotide sequence of cdna encoding a lipase from fusarium heterosporum. | fusarium heterosporum produces a solvent-tolerant lipase. a 1.3-kbp lipase cdna was isolated from the cdna library by colony hybridization with an oligonucleotide probe corresponding to the n-terminal amino acid sequence. nucleotide sequence analysis showed an open reading frame of 999 bp, and the deduced amino acid sequence contained the n-terminal sequence determined by edman degradation and the consensus pentapeptide (-gly-x-ser-x-gly-), which is conserved in lipase, esterase, and serine prot ... | 1994 | 7852271 |
| electrophoretic free mobility and viability of microbial cells: a preliminary study in preparation for space experiments. | electrophoretic free mobilities (efm) of four fungal spores and five bacterial cells were determined in 7 mm triethanolamine/acetate (tea) buffer by means of microscopic electrophoresis (me) and free flow electrophoresis (ffe). spores of aspergillus terreus, penicillium citrinum, gliocladium virens, and rhizopus oryzae had similar efm from 2.2 to 3.1 microns sec-1/v cm-1. the resolution of the spore mixture by ffe was therefore determined to be poor. bacterial cells of salmonella typhimurium lt2 ... | 1994 | 7880880 |
| cerebral mucormycosis associated with intravenous drug use: three case reports and review. | we describe three cases of cerebral mucormycosis in intravenous drug users and review 22 previously reported cases. involvement of the basal ganglia was demonstrated in all but two cases. seven of the 10 patients tested for antibodies to the human immunodeficiency virus (hiv) were seronegative. eight of the 25 patients survived and were discharged from the hospital; for 7 of 10 patients, cultures of brain lesions yielded rhizopus arrhizus. the radiographic findings varied, and in most cases, no ... | 1994 | 7888545 |
| cutaneous infection with rhizopus oryzae and aspergillus niger following bone marrow transplantation. | we describe a case of mixed rhizopus oryzae and aspergillus niger infection in a 38 year-old woman who had undergone allogeneic bone marrow transplantation for multiple myeloma. the fungi were isolated from a necrotic ulcer under a plaster cast stabilizing a pathological fracture of the left humerus. we emphasize the necessity for frequent inspection of any covered area of skin and the use of sterile dressings as far as possible in neutropenic patients. | 1993 | 7907626 |
| improvement of heavy metal biosorption by mycelial dead biomasses (rhizopus arrhizus, mucor miehei and penicillium chrysogenum): ph control and cationic activation. | fungal mycelial by-products from fermentation industries present a considerable affinity for soluble metal ions (e.g. zn, cd, ni, pb, cr, ag) and could be used in biosorption processes for purification of contaminated effluents. in this work the influence of ph on sorption parameters is characterized by measuring the isotherms of five heavy metals (ni, zn, cd, ag and pb) with rhizopus arrhizus biomass under ph-controlled conditions. the maximum sorption capacity for lead was observed at ph 7.0 ( ... | 1994 | 7917420 |
| conformational lability of lipases observed in the absence of an oil-water interface: crystallographic studies of enzymes from the fungi humicola lanuginosa and rhizopus delemar. | considerable controversy exists regarding the exact nature of the molecular mechanism of interfacial activation, a process by which most lipases achieve maximum catalytic activity upon adsorption to an oil water interface. x-ray crystallographic studies show that lipases contain buried active centers and that displacements of entire secondary structure elements, or "lids," take place when the enzymes assume active conformations [derewenda, u., a. m. brzozowski, d. m. lawson, and z. s. derewenda. ... | 1994 | 8014587 |
| current progress in crystallographic studies of new lipases from filamentous fungi. | lipases from filamentous fungi have been studied extensively over many years. they exhibit properties attractive for industrial applications, e.g. in laundry detergents, tanning and paper industries and stereospecific organic synthesis. enzymes from the fungi rhizomucor miehei and geotrichum candidum have been among the first neutral lipases to be characterized structurally by x-ray diffraction methods. in this paper we report a preliminary account of crystallographic studies of three other fung ... | 1994 | 8029211 |
| immunohistochemical identification of aetiological agents of systemic bovine zygomycosis. | the reactivity of a panel of rabbit hyperimmune antisera raised against somatic antigens of three zygomycetes, absidia corymbifera, rhizopus oryzae and rhizomucor (mucor) pusillus was assessed by crossed immunoelectrophoresis (xie) and indirect immunofluorescence (iif) staining of experimentally infected murine tissues. despite a widespread cross-reactivity of the neat antisera, heterologous absorption rendered antisera monospecific as assessed by xie. heterologous absorption also rendered the a ... | 1994 | 8040374 |
| alteration of chain length selectivity of a rhizopus delemar lipase through site-directed mutagenesis. | the coding sequences of the rhizopus delemar lipase and prolipase were altered by oligonucleotide-directed mutagenesis to introduce amino acid substitutions. the resulting mutant enzymes, synthesized by the bacterial host escherichia coli bl21 (de3), were tested for their ability to hydrolyze the triglycerides triolein (to), tricaprylin (tc) and tributyrin (tb). mutagenesis and lipase gene expression were carried out using plasmid vectors derived from previously described recombinant plasmids [j ... | 1994 | 8090057 |
| the normal mycoflora of commodities from thailand. 1. nuts and oilseeds. | a comprehensive study was carried out of the fungi occurring in commodities normally traded in thailand. samples of major commodities were obtained from farmers' stocks and middlemen in major producing areas throughout the country. retail samples were obtained from outlets in and around bankok. samples were divided into two portions, one being examined in bangkok, and the second in sydney. after surface disinfection, fungi were enumerated by direct plating on dichloran rose bengal chloramphenico ... | 1993 | 8110599 |
| zygomycosis and hiv infection. | the severe immunosuppression associated with hiv infection increases susceptibility to opportunistic fungi. we describe a primary gangrenous cutaneous infection caused by rhizopus arrhizus in an hiv-infected intravenous narcotic user. in addition, we review nine reported cases of zygomycosis in hiv-infected patients and discuss the frequency and outcome of zygomycosis in hiv infection. eight of 10 patients were intravenous drug users. cutaneous infection occurred in four patients. another case w ... | 1994 | 8169272 |
| production of extracellular acidic lipase by rhizopus arrhizus as a function of culture conditions. | thermophilic strain of rhizopus arrhizus accumulates an acidic lipase in culture fluid when grown in a medium containing ground nut oil, milk powder and inorganic salts. addition of 2.0% ground nut oil yielded the highest productivity of enzyme. soyabean meal and arabinose were found to be the best nitrogen and carbon sources for enzyme production respectively. addition of metal ions such as mncl2, sncl2 and cacl2 increased the enzyme productivity by 4 fold. the enzyme productivity in the fermen ... | 1993 | 8181953 |