Publications

molecular epidemiology of a major subgroup of arthroderma benhamiae isolated in japan by restriction fragment length polymorphism analysis of the non-transcribed spacer region of ribosomal rna gene.	arthroderma benhamiae vectored by small animals, such as household pets, causes tinea lesions on human skin. the number of tinea cases caused by this species is increasing in japan. we attempted to develop a simple molecular method for strain discrimination, which is expected to be useful in molecular epidemiology. out of the 61 strains of a. benhamiae registered at our institute, 46 a. benhamiae strains showed very high degrees of sequence similarity on cluster analysis of the internal transcri ...	2012	22627305
[dermatomycoses due to pets and farm animals : neglected infections?].	dermatomycoses due to contact with pets and livestock frequently affect children and young adults. zoophilic dermatophytes are the main important causative agents. it has long been known that the often high inflammatory dermatophytoses of the skin and the scalp are caused mostly by microsporum canis. due to an absence of an obligation for reporting fungal infections of the skin to the public health office in germany, an unnoticed but significant change in responsible pathogens has occurred. toda ...	2012	23114507
[classification of dermatophytes by mating type (mat) gene analysis].	trichophyton mentagrophytes is epidemiologically divided into two distinct forms, zoophilic and anthropophilic. the zoophilic isolates of t.mentagrophytes have generally been identified by morphological and biochemical examination as well as through mating experiments. the confirmed teleomorphs of the zoophilic isolates of the t.mentagrophytes complex are arthroderma benhamiae, a. simii and a. vanbreuseghemii. on the other hand, no teleomorph has been identified in an anthropophilic isolate of t ...	2012	23149351
the dermatophyte species arthroderma benhamiae: intraspecies variability and mating behaviour.	arthroderma benhamiae is a zoophilic dermatophyte belonging to the trichophyton mentagrophytes species complex. here, a population of a. benhamiae wild strains from the same geographical area (switzerland) was studied by comparing their morphology, assessing their molecular variability using internal transcribed spacer (its) and 28s rrna gene sequencing, and evaluating their interfertility. sequencing of the its region and of part of the 28s rrna gene revealed the existence of two infraspecific ...	2013	23180475
the first human cases of americano-european race of arthroderma benhamiae infection in japan.		2012	23183215
a simple and rapid method to differentiate arthroderma benhamiae from microsporum canis.	arthroderma benhamiae is increasingly isolated in central europe. in culture, this dermatophyte is difficult to differentiate macroscopically from microsporum canis, which is microscopically also true for weak or non-sporulating strains. although there are valid molecular methods for differentiating between these two dermatophytes, in everyday practice it would be helpful for epidemiological and treatment considerations to have a simple and rapid method available for discrimination.	2013	23194462
a comparative study of nitrilases identified by genome mining.	escherichia coli strains expressing different nitrilases transformed nitriles or kcn. six nitrilases (from aspergillus niger (2), a. oryzae, neurospora crassa, arthroderma benhamiae, and nectria haematococca) were arylacetonitrilases, two enzymes (from a. niger and penicillium chrysogenum) were cyanide hydratases and the others (from p. chrysogenum, p. marneffei, gibberella moniliformis, meyerozyma guilliermondi, rhodococcus rhodochrous, and r. ruber) preferred (hetero)aromatic nitriles as subst ...	2013	23475593
[kerion caused by the zoophilic dermatophyte trichophyton species of arthroderma benhamiae in a child. a new emerging pathogen of dermatomycoses in germany].	trichophyton (t.) species of arthroderma (a.) benhamiae is a new emerging pathogen of dermatomycoses in children and adolescents. this zoophilic fungus seems to be more common than other zoophilic dermatophytes, e. g. microsporum canis transmitted from cats to humans, zoophilic strains of t. interdigitale, and t. verrucosum as cause of ringworm of cattle. trichophyton species of a. benhamiae is transmitted from guinea pigs (cavia porcellus form. domestica) to human beings and causes highly infla ...	2013	24008933
assessment of the cutaneous immune response during arthroderma benhamiae and a. vanbreuseghemii infection using an experimental mouse model.	dermatophytoses are common but poorly understood skin infections. most in vivo studies have been performed using the guinea pig as the experimental animal model, which has several limitations.	2014	24116688
[tinea barbae profunda by arthroderma benhamiae. a diagnostic challenge].	dermatomycoses due to pets and farm animals are often a clinical and diagnostic challenge for dermatologists. a 24-year-old man presented with inflammatory skin changes on his cheeks and chin. because of negative fungal culture and the clinical appearance of a highly inflammatory process, our first diagnosis was a bacterial pyoderma. polymerase chain reaction (pcr) identified arthroderma benhamiae in both the patient and his guinea pig. a. benhamiae is a zoophilic dermatophyte which belongs to t ...	2013	24150818
in-vitro activity of 10 antifungal agents against 320 dermatophyte strains using microdilution method in tehran.	dermatophyte fungi are the etiologic agents of skin infections commonly referred to as ringworm. these infections are not dangerous but as a chronic cutaneous infections they may be difficult to treat and can also cause physical discomfort for patients. they are considered important as a public health problem as well. no information is available regarding the efficacy of antifungal agents against dermatophytes in tehran. therefore, in this study we evaluated the efficacy of 10 systemic and topic ...	2013	24250660
frequent isolation of arthroderma benhamiae from dogs with dermatophytosis.	molecular analysis methods have led to many changes in the taxonomy of dermatophyte species.	2014	24267472
[taxonomic study of clinic isolates of trichophyton in rosario, argentina].	due to the pleomorphism and cultural variability displayed by species of the genus trichophyton, the identification methods based solely on morphological features are usually insufficient for their classification. the goal of the present work was to test a set of phenotypic methods in order to identify fungal isolates that belong to the aforementioned genus. these methods were based on a molecular taxonomic technique used as standard. clinical isolates (56) were used as samples along with 6 refe ...	2014	24401778
mating type gene (mat1-2) of trichophyton verrucosum.	trichophyton verrucosum is a zoophilic dermatophyte species that is the most frequent etiologic agent of bovine dermatophytosis throughout the world. since no teleomorph of t. verrucosum has been found, polymerase chain reaction (pcr) analysis on the genome of t. verrucosum isolated from the czech republic and japan was performed to confirm the presence of a mating type locus in the genome of the fungus and to clarify its classification and ecological characteristics. the mating type gene (mat1- ...	2014	24414097
mycology - an update. part 1: dermatomycoses: causative agents, epidemiology and pathogenesis.	dermatomycoses are caused most commonly by dermatophytes. the anthropophilic dermatophyte trichophyton rubrum is still the most frequent causative agent worldwide. keratinolytic enzymes, e.g. hydrolases and keratinases, are important virulence factors of t. rubrum. recently, the cysteine dioxygenase was found as new virulence factor. predisposing host factors play a similarly important role for the development of dermatophytosis of the skin and nails. chronic venous insufficiency, diabetes melli ...	2014	24533779
[dermatophytes transmitted by pets and cattle].	most inflammatory skin and hair dermatophytoses are caused by one of four zoophilic dermatophyte species: microsporum canis (from cats and dogs), trichophyton verrucosum (from cattle), arthroderma benhamiae (from guinea-pigs) and arthrodermna vanbreuseghemii (generally from cats and dogs). in cases of highly inflammatory tinea corporis, tinea faciae and tinea capitis in humans, it is important to identify with certainty the precise etiologic agent and to examine pets as the possible source of in ...	2014	24772808
trichophyton species of arthroderma benhamiae - a new infectious agent in dermatology.	in germany, infections due to the zoophilic dermatophyte trichophyton (t.) species of arthroderma benhamiae are being more frequently diagnosed. the source of infection of this emerging pathogen overlaps with that of the zoophilic species t. interdigitale. the most common source are guinea pigs. t. species of arthroderma benhamiae causes inflammatory dermatophytosis in children and adolescents. in addition to tinea capitis, it may cause both tinea corporis, tinea manus and frequently tinea facie ...	2014	24981469
exposition of dermatophyte trichophyton mentagrophytes to l-cystine induces expression and activation of cysteine dioxygenase.	cysteine dioxygenase (cdo) is involved in regulation of intracellular cysteine levels by catabolising the cysteine to sulphite and sulphate. in keratinolytic fungi, sulphite is actively excreted to reduce disulphide bridges in keratin before its enzymatic degradation. the pathogenicity role of cdo was confirmed in cysteine-hypersensitive and growth-defective δcdo mutant of arthroderma benhamiae on hair and nails. we analysed the cdo expression regulation in t. mentagrophytes (anamorph of a. benh ...	2014	25040038
nucleotide sequence analysis of beta tubulin gene in a wide range of dermatophytes.	we investigated the resolving power of the beta tubulin protein-coding gene (bt2) for systematic study of dermatophyte fungi. initially, 144 standard and clinical strains belonging to 26 species in the genera trichophyton, microsporum, and epidermophyton were identified by internal transcribe spacer (its) sequencing. subsequently, bt2 was partially amplified in all strains, and sequence analysis performed after construction of a bt2 database that showed length ranged from approximately 723 (t. a ...	2014	25079222
matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry using the vitek ms system for rapid and accurate identification of dermatophytes on solid cultures.	the objective of this research was to extend the vitek ms fungal knowledge base version 2.0.0 to allow the robust identification of clinically relevant dermatophytes, using a variety of strains, incubation times, and growth conditions. first, we established a quick and reliable method for sample preparation to obtain a reliable and reproducible identification independently of the growth conditions. the vitek ms v2.0.0 fungal knowledge base was then expanded using 134 well-characterized strains b ...	2014	25297329
occurrence of arthroderma benhamiae genotype in japan.	in this study, we epidemiologically investigated on clinical isolates of arthroderma benhamiae from humans and animals in japan by internal transcribed spacer (its) region sequence analysis and mating type (mat)-specific pcr. seven of 8 a. benhamiae isolates from a human, rabbits and guinea pigs were identified as group i (white phenotype) by morphological characters and its region sequence analysis. one strain isolated from a degus (octodon degus) produced colonies with few irregular folds and ...	2015	25433509
morphological and physiological features of arthroderma benhamiae anamorphs isolated in northern germany.	the anamorph of arthroderma benhamiae is an upcoming zoophilic dermatophyte that only in recent years has gained importance as a cause of tinea in humans. its identification by conventional methods can cause problems. in this study we have subjected seven genetically confirmed strains of a. benhamiae anamorphs from northern germany recently identified in our laboratory to a comprehensive assessment. their macroscopic and microscopic morphology was checked on various agars and enzyme release stim ...	2015	25530423
molecular identification and classification of trichophyton mentagrophytes complex strains isolated from humans and selected animal species.	species differentiation within trichophyton mentagrophytes complex group currently poses a major diagnostic challenge, with molecular methods increasingly supplementing classical identification based on the morphological and physiological properties of the fungi. diagnostic and epidemiological research aimed at determining the source and means of transmission of dermatophytoses in both humans and animals requires not only species differentiation of isolates but also differentiation within specie ...	2015	25643744
exploring the potential of fungal arylacetonitrilases in mandelic acid synthesis.	the application of arylacetonitrilases from filamentous fungi to the hydrolysis of high concentrations of (r,s)-mandelonitrile (100-500 mm) was demonstrated for the first time. escherichia coli strains expressing the corresponding genes were used as whole-cell catalysts. nitrilases from aspergillus niger, neurospora crassa, nectria haematococca, and arthroderma benhamiae (enzymes nitan, nitnc, nitnh, and nitab, respectively) exhibited different degrees of enantio- and chemoselectivity (amide for ...	2015	25652193
immunoresponses in dermatomycoses.	contact with fungal pathogens initiates a series of host responses beginning with innate immunity, which leads to fungal recognition and microbial killing. the innate immune system also modulates the adaptive immune responses, leading to the establishment of immunological memory and protection against pathogens. in the case of dimorphic fungi such as candida albicans and malassezia, the immune system plays an important role in tolerance and resistance when managing the organisms either as commen ...	2015	25736316
tinea faciei caused by trichophyton mentagrophytes (molecular type arthroderma benhamiae ) mimics impetigo : a case report and literature review of cases in japan.	a 36-year-old female elementary schoolteacher presented with aggregated serous papules surrounded by mild erythema, extending from both nasal wings/nostrils down to the upper lip. no improvement was seen following treatment of the lesions with topical antibiotics for impetigo. potassium hydroxide (koh) direct microscopy confirmed the presence of mycelia, and the infection was diagnosed as tinea faciei. the isolate was identified as trichophyton mentagrophytes using morphological analysis and as ...	2015	25855021
which fungus originally was trichophyton mentagrophytes? historical review and illustration by a clinical case.	several dermatophytes producing numerous pyriform or round microconidia were called trichophyton mentagrophytes. among these dermatophytes are the teleomorph species arthroderma benhamiae, arthroderma vanbreuseghemii and arthroderma simii, and other species such as trichophyton interdigitale, trichophyton erinacei and trichophyton quinckeanum for which only the anamorph is known. confusion exists about which fungus should be really called t. mentagrophytes and about the rational use of this name ...	2015	25912796
a molecular epidemiological survey of clinically important dermatophytes in iran based on specific rflp profiles of beta-tubulin gene.	surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. in this study beta tubulin gene (bt2), was used as the first time in a pcr-rflp format to clarify the distribution of dermatophytosis agents in some parts of iran.	2013	26060667
equine dermatophytosis due to trichophyton bullosum, a poorly known zoophilic dermatophyte masquerading as t. verrucosum.	trichophyton bullosum is a zoophilic dermatophyte from the arthroderma benhamiae complex with a poorly known distribution. in this study, we report a case of dermatophytosis caused by t. bullosum in a 6-year-old male horse who had a skin lesion located in a saddle area. the infection spread rapidly to the upper chest and to both sides of the trunk. the dermatophyte was isolated in culture and identified by sequence analysis of the internal transcribed spacer regions (its rdna). to date, this is ...	2015	26290003
molecular characterization and in vitro antifungal susceptibility of 316 clinical isolates of dermatophytes in iran.	dermatophytosis is a common mycotic infection of the skin, nail, and hair, associated with major public health concern worldwide. various species of dermatophytes show significant differences in susceptibility to antifungals. here, we present the antifungal susceptibility of a large collection of molecularly identified dermatophyte isolates obtained from tropical region of south of iran. a total of 9485 patients clinically suspected to have cutaneous fungal infections were examined. dermatophyto ...	2016	26369643
detection of common dermatophytes in clinical specimens using a simple quantitative real-time taqman polymerase chain reaction assay.	dermatophytes are common fungal pathogens causing mostly superficial infections in humans with a high prevalence worldwide. traditional detection techniques are time-consuming and insensitive, whereas molecular detection methods have proved to be much more rapid and sensitive.	2016	26406273
inactivation of human pathogenic dermatophytes by non-thermal plasma.	non-thermal plasma (ntp) was tested as an in vitro deactivation method on four human pathogenic dermatophytes belonging to all ecological groups including anthropophilic trichophyton rubrum and trichophyton interdigitale, zoophilic arthroderma benhamiae, and geophilic microsporum gypseum. the identification of all strains was confirmed by sequencing of its rdna region (internal transcribed spacer region of ribosomal dna). dermatophyte spores were suspended in water or inoculated on agar plates a ...	2015	26427826
[microsporum canis: current data on the prevalence of the zoophilic dermatophyte in central germany].	microsporum (m.) canis, whose source of infection is mostly cats, is still considered as the most frequently occurring zoophilic dermatophyte in germany and europe. in distinct areas of germany, the zoophilic dermatophyte trichophyton (t.) anamorph of arthroderma (a.) benhamiae also presents a frequent and emerging causative agent of dermatophytoses.	2015	26446665
a 9-month-old girl from iran with extensive erythematous plaques due to trichophyton simii, a zoophilic dermatophyte.	the incidence of dermatophytosis due to trichophyton simii is generally considered to be limited to endemic areas, particularly one area of india. however, the high similarity between the morphological features of atypical t. simii isolates and those of other dermatophytes such as trichophyton interdigitale and arthroderma benhamiae may lead to misidentification of the cause of dermatophytosis in many instances. we investigated a rare case of tinea corporis in a 9-month-old female with extensive ...	2016	26767834
epidemiological aspects of dermatophytosis in khuzestan, southwestern iran, an update.	dermatophytosis is among the most common superficial mycoses in iran. the purpose of this report was to update the clinical and mycological features of human dermatophytosis in the khuzestan, southwestern iran. in the framework of a one-year survey, a total of 4120 skin, hair and nail samples obtained from the outpatients with symptoms suggestive of tinea were analyzed by using direct microscopy, culture and molecular identification methods. strains isolated from cultures were subjected to ampli ...	2016	26886443
hapx mediates iron homeostasis in the pathogenic dermatophyte arthroderma benhamiae but is dispensable for virulence.	for many pathogenic fungi, siderophore-mediated iron acquisition is essential for virulence. the process of siderophore production and further mechanisms to adapt to iron limitation are strictly controlled in fungi to maintain iron homeostasis. here we demonstrate that the human pathogenic dermatophyte arthroderma benhamiae produces the hydroxamate siderophores ferricrocin and ferrichrome c. additionally, we show that the iron regulator hapx is crucial for the adaptation to iron starvation and i ...	2016	26960149
the transcriptional regulators stea and stua contribute to keratin degradation and sexual reproduction of the dermatophyte arthroderma benhamiae.	most superficial fungal infections are caused by dermatophytes, a specialized group of filamentous fungi which exclusively infect keratinized host structures such as hair, skin and nails. since little is known about the molecular basis of pathogenicity and sexual reproduction in dermatophytes, here we functionally addressed two central transcriptional regulators, stea and stua. in the zoophilic species arthroderma benhamiae a strategy for targeted genetic manipulation was recently established, a ...	2017	27170358
[arthroderma benhamiae strains in germany : morphological and physiological characteristics of the anamorphs].	anamorphs of arthroderma (a.) benhamiae, which can cause inflammatory tinea lesions in humans, have been progressively spreading in germany.	2016	27287462
molecular characterization of strains of the trichophyton verrucosum complex from tunisia.	trichophyton verrucosum is the most frequent etiologic agent of cattle dermatophytosis. throughout the world, it was the second most common agent of zoophilic dermatophytes in human. the aim of our study was to evaluate the efficacy of the pcr- rflp and pcr-sequencing methods for the identification and differentiation of t. verrucosum strains.thirty-six clinical strains identified by morphological characteristics as t. verrucosum were isolated from patients referred to parasitology-mycology labo ...	2016	27364650
[trichophyton species of arthroderma benhamiae : clinical therapeutic aspects of a new pathogen in dermatology].	cutaneous infections with trichophyton species of arthroderma (a.) benhamiae are increasingly being detected in germany. this dermatophyte typically causes tinea corporis, tinea faciei or tinea capitis with in part heavy clinical manifestation like kerion celsi. in special cases diagnosis and therapy can be difficult. in this article, four clinical cases are presented, whereby attention is given to special clinical situations and therapeutic aspects with regard to trichophyton species of a. benh ...	2016	27380384
[tinea in the genital area : a diagnostic and therapeutic challenge].	pubogenital tinea or tinea genitalis represents a rare type of dermatophytosis which, however, is increasingly being diagnosed. the mons pubis is affected, but also the outer regions to the penis shaft and the labia together with the groins. pubogenital tinea is a more superficial erythrosquamous type, but strong inflammatory dermatomycoses of the genital area as tinea genitalis profunda ranging to kerion celsi are observed. a total of 30 patients (14-63 years of age, 11 men and 19 women) with p ...	2016	27488308
tinea capitis: erregerspektrum und epidemiologie im zeitlichen wandel.	die tinea capitis ist die häufigste dermatomykose des kindesalters. das erregerprofil zeigt unterschiedliche geographische verteilungsmuster und variiert im laufe der zeit.	2016	27509418
tinea capitis: temporal shift in pathogens and epidemiology.	tinea capitis is the most common type of dermatomycosis in children. its pathogen profile shows geographic variations as well as temporal shifts.	2016	27509419
[tinea corporis due to the rare geophilic dermatophyte microsporum praecox].	a 10-year-old girl suffered from tinea corporis with erythematosquamous and centrifugal growing, sparse itching lesions of her right lower arm. fluorescence optical blankophor® preparation from skin scrapings revealed fungal hyphae. on sabouraud's dextrose agar, the fast growing dermatophyte formed flat, peripheral radiating and convolved colonies with white, slightly yellowish to beige brown stained granular and powdery surface. the reverse side of the colonies was smooth with luminous yellow c ...	2016	27586990
tinea corporis caused by arthroderma benhamiae in a child.		2016	27747922
modeling dermatophytosis in reconstructed human epidermis: a new tool to study infection mechanisms and to test antifungal agents.	dermatophytosis is a superficial fungal infection of keratinized structures that exhibits an increasing prevalence in humans and is thus requesting novel prophylactic strategies and therapies. however, precise mechanisms used by dermatophytes to adhere at the surface of the human epidermis and invade its stratum corneum are still incompletely identified, as well as the responses provided by the underlying living keratinocytes during the infection. we hereby report development of an in vitro mode ...	2016	27760830
rna sequencing-based genome reannotation of the dermatophyte arthroderma benhamiae and characterization of its secretome and whole gene expression profile during infection.	dermatophytes are the most common agents of superficial mycoses in humans and animals. the aim of the present investigation was to systematically identify the extracellular, possibly secreted, proteins that are putative virulence factors and antigenic molecules of dermatophytes. a complete gene expression profile of arthroderma benhamiae was obtained during infection of its natural host (guinea pig) using rna sequencing (rna-seq) technology. this profile was completed with those of the fungus cu ...	2017	27822542