Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen vibrio anguillarum. | the transposon a sequence tn1 containing the ampicillin resistance determinants was transposed from rp4 to a plasmid of the marine fish pathogen vibrio anguillarum. curing experiments in which plasmid loss was determined by analysis of the segregation of the ampicillin resistance phenotype showed the association of virulence with the specific v. anguillarum plasmid class. | 1980 | 6247282 |
| iron uptake system medicated by vibrio anguillarum plasmid pjm1. | plasmid pjm1 from an invasive strain of vibrio anguillarum mediates an iron-sequestering system that is associated with the ability of this bacterium to cause septicemia in marine fishes. this plasmid-mediated iron uptake system was analyzed by using mutations caused by transposon tnl. restriction endonuclease analysis of iron uptake-deficient and -proficient derivatives generated by insertion of tnl and molecular cloning experiments permitted us to localize the plasmid regions involved in the p ... | 1983 | 6313622 |
| identification of vibrio anguillarum with the api 20 e system. | 1983 | 6341781 | |
| [study of various application methods for active immunization of fishes with aeromonas salmonicida, aeromonas punctata and vibrio anguillarum. 1. immunization with aeromonas salmonicida strain 314]. | 1983 | 6352234 | |
| lack of homology between the iron transport regions of two virulence-linked bacterial plasmids. | two plasmids involved in bacterial virulence, the escherichia coli plasmid pcolv-k30 and the vibrio anguillarum plasmid pjm1, have been studied with respect to the iron sequestering systems mediated by these two plasmids. bioassay results show that the two systems are not related functionally because specific iron uptake-deficient mutants in each system cannot be cross-fed by the heterologous bacteria using culture supernatants from iron-proficient strains containing wild-type plasmids. dna hybr ... | 1984 | 6363301 |
| the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water. | a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ... | 1984 | 6395571 |
| nucleotide base sequence of vibrionaceae 5 s rrna. | nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species. | 1984 | 6479333 |
| vibrio anguillarum: influence of temperature, ph, nacl concentration and incubation time on growth. | an analysis of the effect of growth conditions on the growth (o.d. values) of five vibrio anguillarum strains showed that the optima were as follows: ph 7, temperature 25 degrees c, nacl concentration 2%, and o.d. estimates increased with the incubation time. the independent parameters, as well as their interactions significantly influenced the growth of vibrio anguillarum (p less than 0.0001). only the strain-salinity interaction was not always statistically significant. a restriction of the pa ... | 1984 | 6501118 |
| phylogenetic relationships among vibrio anguillarum plasmids. | results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ... | 1984 | 6528000 |
| mitogenicity and adjuvanticity of lipopolysaccharide from a marine bacterium, vibrio anguillarum, in mice. | 1984 | 6529810 | |
| multiflagellate variants of vibrio anguillarum. | an ultrastructural examination of six strains of vibrio anguillarum of varying virulence for eels revealed an apparent correlation between pathogenicity and the possession of more than one flagellum. the relationship between v. anguillarum surface appendages and virulence is discussed. | 1983 | 6631410 |
| susceptibility and immunity to vibrio anguillarum in post-hatching rainbow trout fry, salmo gairdneri richardson 1836. | rainbow trout fry were tested for their susceptibility to experimental infection with vibrio anguillarum, and their ability to mount an immune response against it, from the age of 2 weeks post-hatch onwards. bath challenges were ineffective in inducing vibriosis until 6-8 weeks post-hatch, and then only low levels of specific mortality ensued, even at very high doses. however, at the earliest age tested, 7 weeks post-hatch, the fry were susceptible to infection by an intraperitoneal injection wi ... | 1983 | 6642043 |
| a numerical taxonomic study of species of vibrio isolated from the aquatic environment and birds in kent, england. | a numerical taxonomic study has been carried out to confirm the identity of strains of the family vibrionaceae isolated during an ecological study. a total of 237 strains were studied including 148 from the aquatic environment, 6 from estuarine birds, 1 from sheep faeces, and 61 control cultures. duplicates of 21 of the strains were randomly selected and included to estimate test and operator error. taxonomic resemblance was estimated on the basis of 148 characters using euclidean distance. the ... | 1983 | 6654766 |
| [antitumor activity and immunostimulating effect of culture filtrate of a marine bacterium, vibrio anguillarum, in mice]. | 1983 | 6663452 | |
| vibrio anguillarum: a comparative study of fish pathogenic, environmental, and reference strains. | 1983 | 6675456 | |
| drug resistance and r plasmids in vibrio anguillarum isolated in cultured ayu (plecoglossus altivelis). | two hundred twenty-six strains of vibrio anguillarum collected from cultured ayu ( plecoglossus altivelis ) between 1978 and 1980 were studied for their sensitivities to 10 chemotherapeutics. in order to determine whether the drug-resistant strains possessed transferable r plasmids, they were conjugated with escherichia coli. almost all the strains isolated during the 3 years showed resistance to nalidixic acid (na) and/or furazolidone (nf). na and nf resistance were not transferred to escherich ... | 1984 | 6727708 |
| cellular sugar pattern of vibrio anguillarum. | 1984 | 6748970 | |
| conjugation of drug-resistance plasmids from vibrio anguillarum to vibrio parahaemolyticus. | drug-resistant strains of vibrio anguillarum, a fish pathogen, were isolated from diseased fish in culture ponds. in investigations of these strains, the transfer of resistance to chloramphenicol, tetracycline, and sulfanilamide from multiple resistant organisms to laboratory recipients was observed. the plasmid from v. anguillarum was stably maintained in both recipient strains of vibrio parahaemolyticus and escherichia coli. the plasmids isolated from vibrio anguillarum belong to incompatibili ... | 1982 | 6752666 |
| characterization of plasmids in bacterial fish pathogen. | plasmid profiles of representative fish pathogens, aeromonas salmonicida, aeromonas hydrophila, vibrio anguillarum, pasteurella piscicida, yersinia ruckeri, edwardsiella tarda, and renibacterium salmoninarum, were determined by agarose gel electrophoresis with four different plasmid detection methods. a combination of two methods was required to detect the plasmids present in these strains and to calculate precisely the molecular weights of the plasmids. of 38 strains, 28 harbored one or more pl ... | 1983 | 6822413 |
| molecular factors associated with virulence of marine vibrios isolated from striped bass in chesapeake bay. | on the basis of cultural and biochemical properties as well as dna homology assays, 81 vibrio strains isolated from diseased striped bass and from chesapeake bay water were assigned to eight distinct groups. all organisms belonging to two of the groups were pathogenic for striped bass and were identified as vibrio anguillarum, whereas organisms classified in the other six groups were nonpathogenic and were designated as vibrio spp. unlike the pathogenic v. anguillarum strain 775 isolated in the ... | 1983 | 6840838 |
| antitumor activity of 2-keto-3-deoxyoctonate-free lipopolysaccharide of vibrio anguillarum in mice. | the antitumor activity of a marine bacterium, vibrio anguillarum, against ehrlich carcinoma cells in ddy mice was investigated. the aqueous layer obtained by the hot phenol-water procedure exhibited more antitumor activity than did the middle layer or the phenol layer. this finding indicates that lipopolysaccharide (lps) derived from v. anguillarum exhibits significant antitumor activity. in fact, mice injected with lps obtained by ultracentrifugation and treatment with rnase had a longer mean s ... | 1983 | 6862149 |
| transmission of r plasmids in vibrio anguillarum to vibrio cholerae. | 1983 | 6865805 | |
| the ulcus-syndrome in cod (gadus morhua). iv. transmission experiments with two viruses isolated from cod and vibrio anguillarum. | vibrio anguillarum is a serious fish pathogenic bacterium, but it was not found capable of producing the ulcus-syndrome by different inoculation methods in cod. it was possible to transmit the ulcus-syndrome from cod with the papulo-vesicular stage to apparently healthy cod, when they were kept in the same aquarium. scarification and inoculation with ground papule material lead to development of the ulcus-syndrome. inoculation of rhabdovirus failed to produce the ulcus-syndrome. intracardial ino ... | 1982 | 7177806 |
| outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen vibrio anguillarum 775. | cells of the marine fish pathogen vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. in contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. radioactive from (55fe3+) uptake experiments indicate that this plasmid-linked ability of v. anguillarum cells to grow under conditions of iron limitation is indeed due to a more rapid and efficient iron up ... | 1981 | 7194320 |
| mitogenicity and adjuvanticity of a marine bacterium, vibrio anguillarum, in mice. | the effects of whole cells of three different o serotypes of vibrio anguillarum on the murine immune response were studied. the addition of different doses (1-100 microgram/ml) of v. anguillarum cells, as well as salmonella typhimurium lipopolysaccharide, markedly increased the incorporation of [3h]thymidine into in vitro cultured spleen cells of c57bl/6 mice. all three serotype strains of v. anguillarum were able to induce the mitogenic effect at 10 microgram /ml and 100 microgram/ml, but serot ... | 1981 | 7311889 |
| isolation and characterization of 3-acetamido-3,6-dideoxy-l-glucose from the core oligosaccharides obtained from the aquatic gram-negative bacteria aeromonas hydrophila and vibrio anguillarum. | 1981 | 7332867 | |
| serum resistance and hemagglutination ability of marine vibrios pathogenic for fish. | representative strains of marine vibrios pathogenic for fish were shown to be resistant to the bactericidal activity of normal (nonimmmune) rainbow trout (salmo gairdneri) serum, and loss of this resistance coincided with a marked reduction in virulence. thermal lability and a requirement for mg2+, but not for ca2+, suggested that a mechanism for serum killing was the alternative complement pathway. in the case of vibrio anguillarum, serum resistance was not coded for by the virulence plasmid pj ... | 1981 | 7333667 |
| a plasmid associated with virulence in the marine fish pathogen vibrio anguillarum specifies an iron-sequestering system. | many of the high-virulence strains of the marine fish pathogen vibrio anguillarum isolated from epizootics of the widespread fish disease vibriosis, harbour a specific plamid class which is absent from low-virulence strains. curing experiments have confirmed a link between this specific plasmic class and the ability of v. anguillarum to establish infections. in general, all bacterial virulence factors promote growth in the antagonistic environment of the host defence mechanisms. one line of defe ... | 1980 | 7366725 |
| effects of sodium dichromate exposure on the immune responses of juvenile coho salmon, oncorhynchus kisutch, against vibrio anguillarum. | sublethal exposure of juvenile coho salmon (oncorhynchus kisutch) to cr (as sodium dichromate) in freshwater decreased their disease resistance and serum a-glutinin production against vibrio anguillarum. duplicate disease resistance experiments, in which groups of salmon were exposed to 0.0 or 0.5 mg cr/l for two weeks and then injected in subgroups with one of four tenfold dilutions of live v. anguillarum (ca. 3-4 x 10(2-5) bacteria/.1 ml dose), showed that disease mortality increased with dose ... | 1980 | 7387188 |
| characterization and regulation of the expression of fatb, an iron transport protein encoded by the pjm1 virulence plasmid. | the pjm1-encoded genes fatdcba are essential for iron acquisition via the siderophore anguibactin. sequence analysis indicated that the open reading frame corresponding to the fatb gene possesses domains that are characteristic of periplasmic proteins that bind the ferric siderophore. in this work, a monospecific antiserum against an oligopeptide containing the last 27 amino acids of the carboxy-terminal region from this open reading frame was used to demonstrate that fatb encodes a 35 kda prote ... | 1995 | 7476205 |
| ribotypes and plasmid contents of vibrio anguillarum strains in relation to serovar. | eighty-six strains of the 10 major agglutination types of vibrio anguillarum (serovars o1 to o10) and 6 nontypeable strains of v. anguillarum have been characterized by ribotyping with a probe complementary to 16s and 23s rrna of escherichia coli and by plasmid profile analysis. forty-four different ribotypes were observed with the restriction enzyme hindiii. ribotype similarity was compared by using the dice coefficient (sd), and three significantly different levels of homogeneity within the v. ... | 1993 | 7506897 |
| rapid detection and identification of vibrio anguillarum by using a specific oligonucleotide probe complementary to 16s rrna. | partial 16s rdna from vibrio collection type strains and recent isolates of vibrio-related strains were sequenced and compared with previously published sequences. a 24-base dna oligonucleotide (vav3) was designed and used as a specific probe for detection and identification of vibrio anguillarum. its specificity was tested against collection type strains and environmental isolates and no cross-reaction was found. the probe detected 8 of the 10 v. anguillarum serovars. it was applied to screen d ... | 1994 | 7510943 |
| monoclonal antibodies against vibrio anguillarum o2 and vibrio ordalii identify antigenic differences in lipopolysaccharide o-antigens. | monoclonal antibodies (mabs) that recognize distinct species-specific antigenic epitopes in o-antigens from vibrio anguillarum o2, o2a and certain o2b strains (mab 7b4) and from vibrio ordalii strains (mabs a16 and 7d11) were generated. western immunoblot analysis using these mabs revealed that vibrio strains grown in the presence of fresh rainbow trout blood expressed lipopolysaccharide (lps) with longer (high molecular mass) o-antigens and extracellular capsular layers when compared to strains ... | 1994 | 7527364 |
| a specific oligonucleotide probe based on 5s rrna sequences for identification of vibrio anguillarum and vibrio ordalii. | an oligonucleotide dna probe based on 5s rrna sequence data was constructed for the identification of the fish pathogens, vibrio anguillarum and vibrio ordalii. specificity of the probe was tested in a colony blot hybridization assay. the respective probe was found to be specific for both v. anguillarum and v. ordalii. no cross hybridization was observed against other fish pathogens and the closely related vibrionaceae genera. this specific probe may be useful for rapid identification of v. angu ... | 1995 | 7537929 |
| regulation of angr, a gene with regulatory and biosynthetic functions in the pjm1 plasmid-mediated iron uptake system of vibrio anguillarum. | angr and the product(s) encoded in the trans-acting factor (taf) region are necessary for the full expression of the pjm1 plasmid-mediated anguibactin iron-uptake system in vibrio anguillarum (va). in this report, we analyzed the factors that affect the expression of the angr gene. northern blot analysis showed that angr encodes a 3.1-kb transcript which is expressed only under iron-limiting conditions. measurement of steady-state rna levels show that, under iron-limiting conditions, angr is pos ... | 1995 | 7543065 |
| cloning and expression of rfb genes from vibrio anguillarum serotype o2 in escherichia coli: evidence for cross-reactive epitopes. | vibrio ordalii and vibrio anguillarum o2 express lipopolysaccharide (lps) o antigens containing both specific and cross-reactive epitopes. the localization of these epitopes on the o antigen is not known. we have cloned and expressed the rfb gene cluster for o-antigen synthesis from v. anguillarum o2 (rfbvao2) in escherichia coli. e. coli dh5 alpha containing the recombinant plasmid pam86 expressed o antigens which reacted with polyclonal antisera to v. ordalii and to v. anguillarum o2 lps and w ... | 1995 | 7543885 |
| iron transport genes of the pjm1-mediated iron uptake system of vibrio anguillarum are included in a transposonlike structure. | the pjm1 genes encoding the proteins involved in iron transport in the anguibactin iron uptake system were found to be flanked by insertion sequences in a composite transposonlike structure. these vibrio anguillarum insertion sequences, isv-a1 and isv-a2, are related to is903, is102, and the isvs found in vibrio parahaemoliticus, vibrio mimicus, and non-o1 vibrio cholerae flanking various tdh (thermostable direct hemolysin) genes. the inverted repeats at the ends of isv-a1 and isv-a2 have no mor ... | 1995 | 7568465 |
| antibiotic resistance of vibrio anguillarum, in relation to serovar and plasmid contents. | a total of 520 vibrio anguillarum strains, isolated from fish and the environment, were tested for their sensitivity to 20 different antibiotics. most isolates were of european origin. the results were compared with data on the o-serogroup and plasmid contents. all strains were sensitive to neomycin, spectinomycin, nitrofurantoin, flumequine and oxolinic acid, while most strains were sensitive to streptomycin, oxytetracycline, chloramphenicol, sulphonamides, trimethoprim, sulphonamides with trim ... | 1995 | 7572458 |
| sequence of a novel virulence-mediating gene, virc, from vibrio anguillarum. | previously, the double-transposon (tn) mutant van20 of vibrio anguillarum (va) 775.17b was isolated. this mutant lacked a major surface antigen (msa) suggested to be a lipopolysaccharide (lps) and showed a 10(5)-fold increase in the 50% lethal dose (ld50) when fish were infected intraperitoneally. in this study, the two tn insertion sites within the chromosome were identified, a plasmid insertion mutation was made at each locus in a more virulent strain of va, nb10, and the virulence was analyze ... | 1995 | 7590330 |
| ribotyping and plasmid profiling of vibrio anguillarum serovar o2 and vibrio ordalii. | one hundred and twenty-nine strains of vibrio anguillarum serovar o2 and 14 strains of vibrio ordalii were ribotyped and examined for plasmid contents. a total of 35 different ribotypes were detected. the v. anguillarum serovar o2 strains were divided into 32 different ribotypes. the v. ordalii strains showed three different ribotypes, clearly distinct from those of the v. anguillarum strains. ribotypes were separated into seven clusters, of which one comprised the v. ordalii strains. clustering ... | 1995 | 7592131 |
| the effect of incubation temperature and sodium chloride concentration on the growth kinetics of vibrio anguillarum and vibrio anguillarum-related organisms. | the effect of temperature and nacl concentration on the growth kinetics of vibrio anguillarum and v. anguillarum-related (var) strains was studied. for one wild var strain, nacl concentration interfered with growth temperature parameters, in particular, with the maximum growth temperature but also with the optimum temperature (defined as the temperature at which mumax equals its maximal value muopt), and with muopt itself. for the same strain, optimal growth required the adding of nacl to the me ... | 1995 | 7615418 |
| analysis of antigens present in the extracellular products and cell surface of vibrio anguillarum serotypes o1, o2, and o3. | antigens present in the extracellular products (ecp) and cell walls of strains of vibrio anguillarum of serotypes o1, o2, and o3 isolated from different fish species in distinct geographic areas were characterized. the usefulness of slide agglutination, dot blot assay, and quantitative agglutination for subtyping v. anguillarum serovars was also evaluated. the three serological assays used to establish the serogroups within v. anguillarum isolates demonstrated that serotype o1 constitutes a homo ... | 1995 | 7618861 |
| decrease in culturability of vibrio cholerae caused by glucose. | the culturability of vibrio cholerae o1 serotype inaba strain 569b was decreased by the addition of glucose to cell suspensions in starvation media. a similar effect was observed with sucrose, maltose, and fructose. we term this inhibitory effect glucose shock. it was not observed with arabinose or xylose or with carboxylates, such as acetate and pyruvate. no acidification of the medium occurred in the presence of these carbohydrates. glucose shock was prevented by the addition of nitrogen or ph ... | 1995 | 7618870 |
| characterization of vibrio anguillarum strains isolated from diseased fish in finland. | characterization of v. anguillarum strains (n = 109) isolated from diseased salmonids was performed. eight o serovars were found among the strains. serovar o1 was predominant (90%), while serovars o2, o3, o5, o8, o9, and a new serovar va nt2, were represented by 1 or 2 strains. two strains remained non-typeable. one of these was cross-reactive with several antisera, but had a lps profile identical to that of serovar o8. all serovars showed specific lps profiles. all but 1 of the o1 strains had a ... | 1994 | 7676917 |
| regulation of the expression of bacterial iron transport genes: possible role of an antisense rna as a repressor. | expression of the iron transport gene, fatb and fata, in the bacterium vibrio anguillarum 775 is negatively regulated by the iron concentration in the medium. here, we show that iron represses fatb and fata mrna levels and concomitantly induces the synthesis of an antisense rna (rna alpha). the presence of rna alpha correlates with the inhibition of fata protein synthesis and thus may play a role in the iron repression of fata expression. since the 5' end of rna alpha maps 125 nucleotides upstre ... | 1993 | 7678569 |
| mechanisms for negative regulation by iron of the fata outer membrane protein gene expression in vibrio anguillarum 775. | synthesis of the 86-kda fata outer membrane protein is repressed under iron-rich conditions. complementation of transposition mutants derived from clones containing the pjm1 iron uptake region revealed the existence of an antisense rna, rna alpha. this rna is only expressed under iron-rich conditions and acts as a negative regulator of fata synthesis, with slight but discernible decrease in the steady-state level of fata mrna determined by rnase protection and by northern blot analysis. primer e ... | 1993 | 7683679 |
| antibody specificities of polyclonal rabbit and rainbow trout antisera against vibrio ordalii and serotype 0:2 strains of vibrio anguillarum. | polyclonal rabbit antisera raised against vibrio ordalii and serotype 02 strains of vibrio anguillarum showed extensive cross-reactivity with lipopolysaccharide from these bacterial pathogens of fish when tested in western immunoblot analysis. results with absorbed polyclonal antisera indicated that lipopolysaccharide molecules from these strains had both common and strain-specific antigenic determinants, which allowed the antisera to be used to differentiate between v. ordalii and serotype 02 s ... | 1993 | 7687195 |
| genetic variation in the humoral immune response against vibrio salmonicida and in antibody titre against vibrio anguillarum and total igm in atlantic salmon (salmo salar). | total igm level and antibody titre to vibrio anguillarum o-antigen after bath-vaccination, and specific antibody response to v. salmonicida o-antigen at three different samplings were analysed in family material of atlantic salmon (salmo salar), consisting of 791 fish belonging to 34 maternal full-sib groups within 12 paternal half-sib groups. the fish were immunized twice, and blood samples collected three times. after the third blood sampling, the fish were challenged with v. anguillarum. medi ... | 1994 | 7725631 |
| vaccination in european salmonid aquaculture: a review of practices and prospects. | disease control by vaccination is widely used in european salmonid aquaculture against vibriosis (vibrio anguillarum), cold-water vibriosis (vibrio salmonicida), yersiniosis or enteric redmouth disease (yersinia ruckeri) and furunculosis (aeromonas salmonicida subsp. salmonicida). the vaccines against the vibrio spp. and y. ruckeri have proven effective especially when administered by injection. furunculosis vaccines have been less successful and have relied on combination with potent adjuvants ... | 1995 | 7735870 |
| the plasmid profiles of fish pathogenic isolates of aeromonas salmonicida, vibrio anguillarum, and vibrio ordalii from the atlantic and pacific coasts of canada. | the plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of aeromonas salmonicida, vibrio anguillarum, and vibrio ordalii were examined by agarose gel electrophoresis. bacterial isolates were from disease outbreaks in fish on the atlantic and pacific coasts. resistant isolates were examined when grown in the presence and absence of antibiotic. alkaline lysis methods were used for plasmid isolation. vibrio spp. were predominantly plasmidless, except for a 47-kilobase (kb) plas ... | 1995 | 7736353 |
| genetic transformation of vibrio anguillarum and pasteurella piscicida by electroporation. | vibrio anguillarum and pasteurella piscicida are gram-negative bacteria which are pathogenic for marine fish and we report here the first successful transformation of these two bacteria by electroporation. the optimal conditions for electroporation included a field strength of 12.5 kv cm-1 and a time constant of 5 ms using 0.2-cm cuvettes. with these parameters, three plasmids (psu2718, pcml, pev3) with molecular sizes of 2.6, 5 and 13.7 kb, respectively were successfully transformed into both p ... | 1995 | 7744241 |
| a histidine decarboxylase gene encoded by the vibrio anguillarum plasmid pjm1 is essential for virulence: histamine is a precursor in the biosynthesis of anguibactin. | we have identified and sequenced an hdc gene in the vibrio anguillarum plasmid pjm1 which encodes a histidine decarboxylase enzyme and is an essential component for the biosynthesis of anguibactin. the open reading frame corresponds to a protein of 386 amino acids with a calculated molecular mass of 44,259.69 da. the amino acid sequence has extensive homology with the pyridoxal-p-dependent histidine decarboxylases of morganella morganii, klebsiella planticola, and enterobacter aerogenes. tn3-hoh ... | 1995 | 7752899 |
| fatty acid profiles of "pasteurella" piscicida: comparison with other fish pathogenic gram-negative bacteria. | the fatty acid methyl ester (fame) profiles of "pasteurella" piscicida were determined by gas chromatography and subjected to numerical analysis in comparison with those obtained for vibrio anguillarum, aeromonas salmonicida and pasteurella species of clinical origin. the bacterial species studied shared important characteristics with respect to their fame content: in all of them the saturated and unsaturated fatty acids of 16 carbon atoms were the predominant fatty acids. however, distinguishin ... | 1995 | 7778977 |
| evidence for the existence of distinct populations of vibrio anguillarum serogroup o1 based on plasmid contents and ribotypes. | a total of 103 vibrio anguillarum serogroup o1 strains displaying 15 different plasmid profiles were characterized with respect to biochemical properties and ribotypes. the results confirmed that v. anguillarum o1 is a biochemically homogeneous group. the 103 strains could be allocated to three main clusters with high similarity coefficients. none of the biochemical properties were connected with the presence of plasmids. in total, 12 different ribotypes were demonstrated, with hindiii being use ... | 1995 | 7793949 |
| isolation and structure elucidation of acinetobactin, a novel siderophore from acinetobacter baumannii. | a novel siderophore, called acinetobactin, with both catecholate and hydroxamate functional groups was isolated from low-iron cultures of acinetobacter baumannii atcc 19606. the structure was elucidated by chemical degradation, fast-atom bombardment mass spectrometry and 1h and 13c nmr spectroscopy. acinetobactin was composed of omega-n-hydroxyhistamine, threonine and 2,3-dihydroxybenzoic acid, the last two components forming an oxazoline ring. acinetobactin was structurally related to anguibact ... | 1994 | 7802543 |
| characterization of turbot (scophthalmus maximus) associated bacteria with inhibitory effects against the fish pathogen vibrio anguillarum. | more than 400 isolates from the intestine and the external surface of farmed turbot, as well as from fish food and hatchery water were screened for inhibitory effects against the fish pathogen vibrio anguillarum hi 11345 and seven other fish pathogens. the bacteria with inhibitory effects were then characterized with regards to their sites of colonization, especially the intestinal regions and sites within each region. no correlations between the different biochemical phena, site of colonization ... | 1994 | 7866724 |
| monoclonal antibodies to turbot (scophthalmus maximus) immunoglobulins: characterization and applicability in immunoassays. | five monoclonal antibodies (mabs) to immunoglobulins (igs) of the turbot scophthalmus maximus were produced and characterized. all the mabs (denominated ur1, ur3, ur4, ur6 and ur7) are of isotype igg1/kappa and show good anti-turbot ig reactivity in enzyme-linked immunosorbent assay (elisa) and immunoblotting. results of competitive elisa and immunoblotting analysis indicate that these five mabs react with at least three different epitopes on the turbot ig h chain. except in the case of ur1, rea ... | 1994 | 7941313 |
| a medium for presumptive identification of vibrio anguillarum. | a medium (vam) for differentiation of vibrio anguillarum is described. the presence of bile salts, the high ph, and the high nacl concentration select mainly for vibrio species. the high salinity and the ampicillin select for a fraction of vibrio species, and sorbitol fermentation differentiates among those vibrios still able to grow. one hundred ninety-seven of 227 strains of v. anguillarum were identified with this medium. only 3 of 66 strains of vibrio that were not v. anguillarum or v. angui ... | 1994 | 8017947 |
| chromosome-mediated 2,3-dihydroxybenzoic acid is a precursor in the biosynthesis of the plasmid-mediated siderophore anguibactin in vibrio anguillarum. | we have isolated a recombinant clone harboring the chromosomal aroc gene, encoding chorismate synthase, from vibrio anguillarum 775 by complementation of the escherichia coli aroc mutant ab2849 which was transfected with a cosmid gene bank of the plasmidless v. anguillarum h775-3. the nucleotide sequence was determined, and an open reading frame that corresponds to a protein of 372 amino acids was found. the calculated mass of 40,417 da was correlated with the size of the v. anguillarum aroc pro ... | 1994 | 8021209 |
| characteristics of porin-like major outer membrane proteins of listonella anguillara serotypes j-o-1, -2 and -3. | major outer membrane protein (momp) was prepared from fish pathogen listonella anguillara. triton x-100 treatment could extract the momp from the bacterium but not from escherichia coli, suggesting loose association of the momp of l. anguillara to the membrane. properties of purified momp from l. anguillara were similar to omp c porin of e. coli. similar antigenicity of the porin like-momp was found among different serotypes of l. anguillara, although the molecular sizes of the momp were differe ... | 1994 | 8038711 |
| structural and functional analyses of mutant fur proteins with impaired regulatory function. | vibrio anguillarum fur mutants, 775met9 and 775met11, were characterized. v. anguillarum 775met9 had a change of d to g at position 104 located in the carboxy terminus resulting in impaired fur activity. computer analysis predicts perturbation of an alpha-helix in the carboxy terminus which may interfere with fur protein conformation. strain 775met11 had a change in the start codon resulting in no protein synthesis. the mutants are unstable, and reversion to the wild type occurs frequently. | 1994 | 8051025 |
| a microbiological assay for determining sarafloxacin and oxolinic acid concentrations in atlantic salmon plasma. | a microbiological assay was developed to quantify the concentrations of the quinolone antibiotics sarafloxacin and oxolinic acid in atlantic salmon (salmo salar) plasma. the assay was a modification of the association of official analytical chemists (aoac) method for antibiotic concentrations in feeds using disk diffusion and a lawn of vibrio anguillarum atcc 19264 as the test organism. with these modifications, sensitivities of 0.04 microgram/ml for sarafloxacin and 0.125 micrograms/ml for oxol ... | 1994 | 8065273 |
| diseases and injuries associated with mortality of hatchery reared baltic cod (gadus morhua l.) larvae. | a cod hatching plant was established in 1992 on the island of bornholm in the baltic sea in order to elucidate the possibilities for restocking of cod fry in this brackishwater system. the disease prevalence in 3 batches of hatchery-reared yolksac larvae from the baltic cod (gadus morhua l.) was monitored during the posthatch period. high prevalences of bacteriosis/mycosis, lordosis/scoliosis, injuries and protozoan endoparasitism were recorded. vibrio sp. and vibrio anguillarum serovar 04, 06, ... | 1993 | 8147291 |
| sequence determination of rrna genes of pathogenic vibrio species and whole-cell identification of vibrio vulnificus with rrna-targeted oligonucleotide probes. | a comparative analysis of seven new 16s rrna gene sequences of pathogenic vibrio species with previously published vibrio sequences confirmed that vibrio vulnificus represents a group that is not closely related to the core organisms of the genus vibrio. in addition, we found that v. vulnificus, listonella (vibrio) anguillarum and vibrio diazotrophicus branch off separately from the core group. a comparison of the 16s rrna gene sequences of v. vulnificus strains belonging to biotypes 1 and 2 rev ... | 1994 | 8186099 |
| phylogenetic relationships between some members of the genera deleya, halomonas, and halovibrio. | the genera halomonas and deleya, which constitute the family halomonadaceae, are difficult to differentiate on the basis of phenotypic and chemotaxonomic attributes. dna-rrna hybridization studies have indicated that some halomonas spp. have the same level of relationship to the type species of the genus deleya as some deleya spp. a phylogenetic analysis of the 16s rrna sequences of seven members of the halomonadaceae indicated that the members of the genera halomonas and deleya do not form sepa ... | 1993 | 8240950 |
| characterization of the vibrio anguillarum fur gene: role in regulation of expression of the fata outer membrane protein and catechols. | the chromosomally encoded vibrio anguillarum fur gene was characterized. the amino acid sequence of the fur protein showed a very high degree of homology with those of v. cholerae and v. vulnificus. the degree of homology was lower, although still high, with the escherichia coli and yersinia pestis fur amino acid sequences, while the lowest degree of homology was found with the pseudomonas aeruginosa fur protein. the c-terminal portion of fur is the least conserved region among these fur protein ... | 1994 | 8282699 |
| a single amino acid change in angr, a protein encoded by pjm1-like virulence plasmids, results in hyperproduction of anguibactin. | the siderophore anguibactin is produced in vivo in a diffusible form and is an important factor in the virulence of vibrio anguillarum. the natural isolate v. anguillarum 531a is a hyperproducer of anguibactin when compared with the prototype strain v. anguillarum 775. the angr gene was found to be responsible for this difference in levels of anguibactin produced. nucleotide sequence analysis showed that the angr531a differed in a single nucleotide from the angr775 present in the prototype plasm ... | 1993 | 8335354 |
| host range and transfer efficiency of incompatibility group hi plasmids. | hi plasmids are distinguished by their thermosensitive mode of conjugation (transfer efficiency is optimal at 22-30 degrees c) and their capacity to encode multiple antibiotic resistance. these traits have implicated hi plasmids as potential vectors in the dissemination of antibiotic resistance among pathogenic and indigenous bacterial species in water and soil environments. we compared the transfer efficiency of hi plasmids with that of plasmids from 13 other incompatibility groups at 37, 24, a ... | 1993 | 8358670 |
| high-molecular-weight protein 2 of yersinia enterocolitica is homologous to angr of vibrio anguillarum and belongs to a family of proteins involved in nonribosomal peptide synthesis. | the iron-regulated irp2 gene is specific for the highly pathogenic yersinia species and encodes high-molecular-weight protein 2 (hmwp2). despite the established correlation between the presence of hmwp2 and virulence, the role of this protein is still unknown. to gain insight into the function of hmwp2, the entire coding sequence and the promoter of irp2 were sequenced. two putative -35 and -10 promoter sequences were identified upstream of a large open reading frame, and two potential fur-bindi ... | 1993 | 8366034 |
| the tonb-dependent ferrichrome receptor fcua of yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity. | a yersinia enterocolitica receptor mutant was isolated which is impaired in ferrichrome uptake. the receptor-encoding gene fcua was cloned in escherichia coli k-12. a fcua mutant of y. enterocolitica could be complemented by the cloned dna fragment. the fcua-encoding region was sequenced and an open reading frame encoding 758 amino acids including a signal sequence of 36 amino acids was found. fcua shared 34.6% amino acid sequence homology with fata, the anguibactin receptor of vibrio anguillaru ... | 1993 | 8384682 |
| characterization of a novel chromosomal virulence locus involved in expression of a major surface flagellar sheath antigen of the fish pathogen vibrio anguillarum. | the fish pathogenic bacterium vibrio anguillarum 775.17b was mutated by the use of transposon tn5-132. two hundred independent exconjugants were isolated and screened for a reduction of virulence in experimental infections of rainbow trout (onchorhynchus mykiss). two of these exconjugants, van20 and van70, showed a significant reduction in virulence after both intraperitoneal and immersion infections. the avirulent mutants showed no loss of any previously suggested virulence determinants of v. a ... | 1993 | 8388864 |
| production and specificity of poly- and monoclonal antibodies raised against shewanella putrefaciens. | polyclonal antibodies were raised in rabbits and mice against shewanella putrefaciens. murine monoclonal antibodies were produced against the type strain (atcc 8071) as well as wild type strains isolated from fish products. the specificities of four polyclonal and 12 monoclonal antibodies were tested by dot-blotting, an indirect and a competitive elisa against 16 gram-negative strains; including six strains of s. putrefaciens and one strain of pseudomonas rubescens (nc 10695). all polyclonal ant ... | 1993 | 8486552 |
| development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum. | a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ... | 1995 | 8548693 |
| classification of fish-pathogenic vibrios based on comparative 16s rrna analysis. | no systematic classification of fish-pathogenic vibrios has been accomplished previously despite the use of serological, physiological, and genetical classification systems. in this study, a comparative 16s rrna analysis of 34 strains (representing seven species) of fish-pathogenic vibrios was performed. the 16s rrna sequences were obtained by using reverse transcriptase. nearly complete sequences were obtained for nine strains. on the basis of the results of this analysis, the remaining strains ... | 1995 | 8590667 |
| identification of heme-binding proteins in the cell membranes of vibrio anguillarum. | two strains of vibrio anguillarum belonging to o1 and o2 serotypes were examined for their ability to bind hemin and hemoglobin. whole cells as well as membrane extracts from both strains could clearly bind hemin and hemoglobin constitutively. hemoglobin binding was completely inhibited by a 100-fold excess of unlabelled hemoglobin and also by hemin, suggesting the existence of specific receptors for heme groups in the cell membranes. several hemin-binding and hemoglobin-binding bands with simil ... | 1996 | 8595867 |
| meso-substituted cationic porphyrins as efficient photosensitizers of gram-positive and gram-negative bacteria. | previous studies on the photosensitization of bacterial cells with different neutral or negatively charged porphyrins and phthalocyanines have demonstrated that, although gram-positive bacteria are efficiently photoinactivated, gram-negative bacteria become photosensitive only after modification of the permeability of their outer membrane. the results described in this paper show that two meso-substituted cationic porphyrins, namely tetra(4n-methyl-pyridyl)porphine tetraiodide and tetra(4n,n,n-t ... | 1996 | 8622178 |
| flagellin a is essential for the virulence of vibrio anguillarum. | a flagellin gene from the fish pathogen vibrio anguillarum was cloned, sequenced, and mutagenized. the dna sequence suggests that the flaa gene encodes a 40.1-kda protein and is a single transcriptional unit. a polar mutation and four in-frame deletion mutations (180 bp deleted from the 5' end of the gene, 153 bp deleted from the 3' end of the gene, a double deletion of both the 180- and 153-bp deletions, and 942 bp deleted from the entire gene) were made. compared with the wild type, all mutant ... | 1996 | 8631707 |
| vibrio trachuri sp. nov., a new species isolated from diseased japanese horse mackerel. | a new species, vibrio trachuri sp. nov., was isolated from the cultured japanese horse mackerel (trachurus japonicus). these vibrio were gram negative, motile rods and formed yellow colonies on btb teepol and tcbs plate, turned tsi medium to yellow and was sensitive to 150 microm o/129 (2,4-diamino-6,7-diisopropyl pteridine phosphate) like listonella anguillarum which has been described as vibrio anguillarum. however, the results of vp test and decarboxylation of lysine or dihydrolation of argin ... | 1995 | 8657009 |
| vibriosis in rainbow trout cultured in the krka estuary, croatia: occurrence and comments. | the frequency of vibriosis in cultured rainbow trout, maintained under different rearing conditions in the krka estuary, was examined over a 6 yr period. annual studies commenced regularly in october and ended in june of the following year. every month during study periods, 37-75 trouts were randomly taken from each of 4 farm sites for routine examination. twenty fish from these samples were subsequently employed for bacteriological analyses. based on morphological and biochemical properties, th ... | 1996 | 8686134 |
| antisense rna, fur, iron, and the regulation of iron transport genes in vibrio anguillarum. | the negative regulation of the expression of iron transport genes fata and fatb in vibrio anguillarum is mediated by a chromosome-encoded fur protein and a plasmid pjm1-derived antisense rna (rnaalpha), which is preferentially expressed under iron-rich conditions. in this work, we characterized the rnaalpha promoter region, and by using promoter fusion and rifampicin experiments we were able to demonstrate that iron regulates rnaalpha synthesis posttranscriptionally by stabilizing rnaalpha half- ... | 1996 | 8702549 |
| physical and structural characterization of yersiniophore, a siderophore produced by clinical isolates of yersinia enterocolitica. | clinical isolates of yersinia enterocolitca, which belong to mouse-lethal serotypes, produce the siderophore yersiniophore. siderophore production was shown to be iron regulated and to reach maximum production in late log phase. yersiniophore is a fluorescent siderophore with maximum excitation at 270 nm and a major emission peak at 428 nm. absorption maxima were seen at 210 and 250 nm with a low broad peak from 280 to 320 nm. purification of unchelated yersiniophore for structural analysis was ... | 1996 | 8744899 |
| identification and characterization of additional flagellin genes from vibrio anguillarum. | previously, the flagellar filament of vibrio anguillarum was suggested to consist of flagellin a and three additional flagellin proteins, flab, -c, and -d. this study identifies the genes encoding flab, -c, and -d and a possible fifth flagellin gene that may encode flae. the flagellin genes map at two separate dna loci and are most similar to the four polar flagellin genes of vibrio parahaemolyticus, also located at two dna loci. the genetic organization of these two loci is conserved between bo ... | 1996 | 8752337 |
| characterization of a porin from the outer membrane of vibrio anguillarum. | the outer membranes of the 10 serovars of vibrio anguillarum showed a common major protein with a size of around 40 kda. antibodies against the major outer membrane protein (momp) of v. anguillarum ao18 (serovar o1) cross-reacted with the momps of all the other serovars but not with the outer membrane proteins of escherichia coli. the momp of v. anguillarum serovar o1 was isolated, reconstituted to two-dimensional crystals, and structurally characterized by electron microscopy and image processi ... | 1996 | 8763947 |
| isolation of heme-binding proteins from vibrio anguillarum using affinity chromatography. | using affinity chromatography techniques, several hemin- and hemoglobin-binding proteins of 97, 56 and 39 kda were isolated from cell envelopes of vibrio anguillarum strain h775-3 (serotype o1) and of 56, 46 and 37 kda from strain rv22 (serotype o2). all these proteins were isolated under iron-rich as well as iron-poor conditions. proteins of 39 kda in h775-3 and of 37 kda in rv22 isolated by hemin affinity could also bind biotinylated hemoglobin after being transferred to nitrocellulose, which ... | 1996 | 8764506 |
| antisense rna regulation of the fatb iron transport protein gene in vibrio anguillarum. | we have recently identified an antisense rna (rna alpha) that regulates the expression of the fata iron transport gene encoding the outer membrane receptor for the iron-anguibactin complex. in this work, we demonstrate that rna alpha also inhibits the expression of fatb, which encodes a 35 kda iron transport protein and has domains homologous to other periplasmic transport proteins. the expression of fata and fatb is repressed under iron-rich conditions, in which rna alpha is induced. rna alpha ... | 1995 | 8801428 |
| application of a modified disc diffusion technique to antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida clinical isolates. | two techniques for antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida strains were compared. the first method was the reference test that determines minimal inhibitory concentrations (mic); the second was a modified diffusion test that measures the inhibitory concentrations in diffusion (icd) by carrying out the diffusion test with five discs of differing contents. icd measurement was not applicable for the susceptibility testing of oxytetracycline and sulfadime ... | 1996 | 8828130 |
| chemotactic motility is required for invasion of the host by the fish pathogen vibrio anguillarum. | the role of the flagellum and motility in the virulence of the marine fish pathogen vibrio anguillarum was examined. non-motile mutants were generated by transposon mutagenesis. infectivity studies revealed that disruption of the flagellum and subsequent loss of motility correlated with an approximate 500-fold decrease in virulence when fish were inoculated by immersion in bacteria-containing water. however, the flagellar filament and motility were not required for pathogenicity following intrap ... | 1996 | 8830252 |
| kinetics of adhesion of selected fish-pathogenic vibrio strains of skin mucus of gilt-head sea bream (sparus aurata l.). | the kinetics of adhesion of vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. a modified langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (vibrio alginolyticus dp1he4 and vibrio anguillarum-like dc12r8 and dc12r9) showed a saturation kinetics except for v. alginolyticus (can), which showed a proportional adsorption kinetics. the adhesive capability for skin mucus does not seem to be an essential viru ... | 1996 | 8837420 |
| overcoming a defect in generalized recombination in the marine fish pathogen vibrio anguillarum 775: construction of a reca mutant by marker exchange. | a defect in generalized recombination has prevented the use of marker exchange for the construction of specific chromosomal mutations in the marine fish pathogen vibrio anguillarum 775. through the use of large segments of homologous dna, we were successful in overcoming this defect and used marker exchange to construct a reca mutant of v. anguillarum h775-3. a recombinant cosmid carrying the reca gene of v. anguillarum 775 in the center of a 25-kb cloned dna insert was isolated by complementati ... | 1996 | 8837428 |
| cloning and detection of the hemolysin gene of vibrio anguillarum. | a 5 kb dna fragment encoding a hemolysin was cloned from the fish pathogenic bacterium vibrio anguillarum using the cosmid vector charomid9-36. an open reading frame of the hemolysin gene (vah1) was 2253 bp and corresponded to a protein of 751 amino acid residues. the deduced amino acid sequence of the vah1 gene and the previously reported vibrio cholerae ei tor hemolysin, v. vulnificus cytolysin-hemolysin, aeromonas hydrophila ahh1 hemolysin, and a. salmonicida ash4 hemolysin showed a significa ... | 1996 | 8878014 |
| the angr protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in vibrio anguillarum. | vibrio anguillarum virulence is associated with the presence of a plasmid-mediated iron-uptake system expressed under iron-limiting conditions, which consists of the siderophore anguibactin and specific iron-transport proteins. this system is maximally expressed under iron-limiting conditions and requires the angr protein that acts as a positive regulator of anguibactin biosynthesis and also possess an ente-like enzymatic function that may play a role in anguibactin biosynthesis. in this work, w ... | 1996 | 8899715 |
| structural studies of the lipopolysaccharide o-antigen and capsular polysaccharide of vibrio anguillarum serotype o:2. | vibriosis caused by vibrio anguillarum affects salmonid and marine fish species worldwide and is considered to be one of the most serious threats to the success of commercial fish farming. in the course of this study, it was found that v. anguillarum serotype o:2 strains produce an acidic capsular polysaccharide having the identical structure to that of the o-chain polysaccharide. one-dimensional and two-dimensional nuclear magnetic resonance techniques, together with partial hydrolysis and vari ... | 1996 | 8901266 |
| morphological, physico-chemical and biological variations in vibrio anguillarum cultured at low osmolarity. | the authors studied the morphological, biochemical, physico-chemical and biological characteristics of vibrio anguillarum cultured on different growth conditions, characterized by low osmolarity and high temperature (37 degrees). one culture was subcultured for several days in tryptone soya agar with 0.5% nacl at 37 degrees c incubation until the cell morphology was stabilized. the low osmolarity, through an osmotic shock, induced remarkable morphological modifications in the strain, evidenced b ... | 1996 | 8914133 |
| immunological reactivity of vibrio anguillarum sero-subgroups o2a and o2b, and comparison of their lipopolysaccharide profiles. | one hundred and twenty-nine vibrio anguillarum serogroup o2 strains were compared by slide agglutination and western blotting for their lipopolysaccharide (lps) structure. the strains showed six different lps profiles, four different reaction patterns in western blotting, and four different kinds of reaction in slide agglutination, when both unabsorbed and absorbed anti-o2a and anti-o2b sera were used. all in all, nine different groups were detected when the combination of these three methods wa ... | 1997 | 8939800 |
| plasmid profiles, restriction fragment length polymorphisms and o-serotypes among vibrio anguillarum isolates. | a total of 279 vibrio anguillarum strains were serotyped and examined for plasmid content. plasmids were subjected to digestion with restriction enzymes. most strains belonged to serogroup o1 (39%) and o2 (16%). in total 164 strains (53%) carried plasmids. of the o1 and o2 isolates, 92% and 30%, respectively, carried one or more plasmids. restriction fragment length polymorphism (rflp) analysis of plasmid dna indicated that plasmids belonged to several groups. each group seemed to be restricted ... | 1996 | 8972671 |
| antigenicity and n-terminal amino acid sequence of a 35 kda porin-like protein of listonella (vibrio) anguillarum: comparison among different serotypes and other bacterial species. | listonella (vibrio) anguillarum, an important fish pathogen, is divided into 10 serotypes according to o-antigens present on the outer membrane. however, the biochemical and immunological properties of porin proteins have not been reported. in this study, the antigenicity and n-terminal amino acid sequence of the 35 kda porin-like-major outer membrane protein (omp35la) were compared among different serotypes of l. anguillarum as well as other bacteria. in western blotting analysis, antisera agai ... | 1996 | 8987710 |
| detection of vibrio anguillarum by a sandwich enzyme-linked immunosorbent assay performed with monoclonal antibodies. | monoclonal antibodies (mabs) against v. anguillarum were produced and characterized by western blotting analysis, competitive binding assays and cross-reactivity tests. their ability to detect v. anguillarum in a liquid culture was tested in a sandwich enzyme-linked immunosorbent assay (elisa) performed with different combinations of these mabs used as capture or tracer antibodies. one combination was selected as the most suitable for diagnostic applications, showing the highest sensitivity and ... | 1996 | 9011153 |
| typing of the fish pathogen listonella (vibrio) anguillara by pyrolysis mass spectrometry. | twenty-eight representatives of listonella (vibrio) anguillara serovars o1, o2 and o3 were compared by curie-point pyrolysis mass spectrometry (pyms). the representatives of serovars o1 and o3 formed discrete, homogeneous groups in ordination plots of the pyms data. strains from serovar o2 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar o2, and the other included two strains which showed cross-reacti ... | 1997 | 9060156 |
| quorum sensing in vibrio anguillarum: characterization of the vani/vanr locus and identification of the autoinducer n-(3-oxodecanoyl)-l-homoserine lactone. | certain gram-negative pathogens are known to control virulence gene expression through cell-cell communication via small diffusible signal molecules termed autoinducers. this intercellular signal transduction mechanism termed quorum sensing depends on the interaction of an n-acylhomoserine lactone (ahl) auto-inducer molecule with a receptor protein belonging to the luxr family of positive transcriptional activators. vibrio anguillarum is a gram-negative pathogen capable of causing a terminal hem ... | 1997 | 9139920 |
| [recent knowledge on fish pathogenic bacteria aeromonas salmonicida, listonella anguillara and cytophaga columnaris, and their virulence factors]. | 1997 | 9155200 |