Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| efficacy of furunculosis vaccines in turbot, scophthalmus maximus (l.): evaluation of immersion, oral and injection delivery. | the commercial furunculosis vaccine aquavac furovac 5 and an autogenous vaccine, based on the challenge strain, induced immune protection in turbot, scophthalmus maximus (l.), as shown in challenge tests 120 days post-immunization by injection (relative percentage of survival, rps = 72-99%). this protective effect lasted for at least 6 months post-immunization at appreciable levels (rps = 50-52%). neither the autogenous vaccine nor the commercial vaccine was able to induce significant levels of ... | 2005 | 15752277 |
| experimental infection of turbot, scophthalmus maximus (l.), by aeromonas salmonicida subsp. achromogenes and evaluation of cross protection induced by a furunculosis vaccine. | turbot was shown to be sensitive to injection challenges by aeromonas salmonicida subsp. achromogenes (asa). a systemic disease was induced and the bacterium was isolated from various internal organs. histopathological changes involved haemorrhages, necrosis and degeneration in skin and muscle, haemorrhages and necrosis in kidney, degeneration in the heart muscle, and fusion of the secondary gill lamellae. a polyvalent commercial salmon vaccine, containing a. salmonicida subsp. salmonicida as on ... | 2005 | 15752279 |
| characterization of an iridovirus detected from cultured turbot scophthalmus maximus in korea. | juvenile turbot scophthalmus maximus that became sick during an outbreak of disease at mariculture facilities at go-chang, korea, in 2003, were examined to identify the cause of the disease. the fish had pale body color, an enlarged abdomen, protruding eyes, an enlarged spleen and kidney, and pale gills and/or liver. histopathogical examination revealed basophilic enlarged cells in the kidney, spleen, gills, heart, stomach, intestine, liver, pancreas and skin. hexagonal viral particles with a di ... | 2005 | 15918481 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| vaccination strategies to prevent emerging diseases for spanish aquaculture. | in recent years, three serious diseases have emerged in spanish aquaculture. these are lactococcosis caused by lactococcus garvieae, which is of economical importance in rainbow trout (oncorhynchus mykiss); pseudomonadiasis caused by pseudomonas anguilliseptica which affects gilthead seabream (sparus aurata) and turbot (scophthalmus maximus); and flexibacteriosis caused by tenacibaculum maritimum which became a devastating problem in the emerging culture of sole (solea spp). to obtain useful inf ... | 2005 | 15962472 |
| vibrio splendidus biotype 1 as a cause of mortalities in hatchery-reared larval turbot, scophthalmus maximus (l.). | to characterize bacteria associated with turbot larvae feeding on artemia and identify pathogens causing mortalities in larvae. | 2005 | 16033454 |
| molecular cloning and expression analysis of interferon regulatory factor-1 (irf-1) of turbot and sea bream. | the interferon regulatory factor (irf) family comprises transcription factors that regulate the expression of interferon and interferon-related cytokines. using the race technique, we have determined the complete cdna sequence of turbot (scophthalmus maximus) and sea bream (sparus aurata) irfs. these sequences shared characteristics with other irfs of fish, mammals and birds, and showed high similarity with irf-1. indeed, they were included in the irf-1 cluster of the phylogenetic tree construct ... | 2006 | 16112733 |
| isolation in cell culture and detection by pcr-based technology of ipnv-like virus from leucocytes of carrier turbot, scophthalmus maximus (l.). | a non-destructive procedure was utilized to determine the infectious pancreatic necrosis virus (ipnv) status of an apparently healthy turbot broodstock. blood samples were used to detect ipnv by reverse transcriptase-polymerase chain reaction (rt-pcr), southern blot hybridization and nested pcr. in addition, viral isolation from turbot leucocytes was performed. around 22% of the fish were ipnv positive by rt-pcr, and this increased to close to 60% when nested pcr was performed. the present repor ... | 2005 | 16336472 |
| experimental susceptibility of atlantic cod, gadus morhua (l.), and atlantic halibut, hippoglossus hippoglossus (l.), to different genotypes of viral haemorrhagic septicaemia virus. | viral haemorrhagic septicaemia (vhs) is a well-characterized disease of rainbow trout, oncorhynchus mykiss, which has also caused economic losses in marine turbot farms in the british isles. we have previously demonstrated that turbot, scophthalmus maximus, are susceptible to isolates of viral haemorrhagic septicaemia virus (vhsv) that are endemic in the marine environment, highlighting a potential risk to marine aquaculture. given the increasing interest in the intensive rearing of additional a ... | 2005 | 16336475 |
| [identification and phylogenetic study of pathogenic bacteria causing ulcer disease of cultured turbot (scophthalmus maximus)]. | an outbreak of ulcer disease of the cultured turbot scophthalmus maximus l. occurred in an indoor farm in haiyang, shandong province in the sweltering august of 2004 and caused significant mortality. at the beginning, infected turbots displayed sluggish swimming and anorexia. several days later, the turbot eyes swelled, the fins and tail turned red, and the back gradually ulcerated. dissection of the moribund fish showed that the ulcerative gills paled, the liver becamed bloodshot, the kidney an ... | 2005 | 16342752 |
| production of eicosapentaenoic acid (20:5 n-3) by vibrio pelagius isolated from turbot (scophthalmus maximus (l.)) larvae. | fourteen bacterial strains isolated from turbot, scophthalmus maximus (l.), larvae were screened for eicosapentaenoic acid (20:5 n-3) (epa) production. gas chromatography analysis revealed that one bacterial species, vibrio pelagius, contained a high proportion of epa in cellular lipid. this finding was confirmed by gas chromatography-mass spectrometry analysis. a higher concentration of epa was detected when the bacterium was cultured at 4 degrees c. | 1992 | 16348815 |
| comparative susceptibility of turbot scophthalmus maximus to different genotypes of viral haemorrhagic septicaemia virus. | viral haemorrhagic septicaemia (vhs) disease has exerted a significant impact on the development of turbot aquaculture in the british isles. the source of such outbreaks is believed to be naturally occurring marine isolates of viral haemorrhagic septicaemia virus (vhsv), which are endemic in the marine environment of northern europe. genetic studies have classified these marine vhsv isolates into genotypes based on their geographic rather than host-species origin. this study set out to explore t ... | 2005 | 16385805 |
| pathology of edwardsiella tarda infection in turbot, scophthalmus maximus (l.). | macroscopic and histopathological changes in cultured turbot, scophthalmus maximus (l.), in spain caused by infection with edwardsiella tarda are described. eye tumefaction, inflammation, haemorrhages, ascites and the presence of a purulent fluid were the main macroscopic lesions observed. histopathological lesions were found in the kidney, spleen and liver. in the kidney and spleen these were characterized by a severe apostematous inflammatory reaction, with a large number of abscesses. the liv ... | 2006 | 16436119 |
| involvement of vamp-2 in exocytosis of il-1 beta in turbot (scophthalmus maximus) leukocytes after vibrio anguillarum infection. | vibrio anguillarum is a major pathogen threatening the fish aquaculture in china. infection of cultivated turbot (scophthalmus maximus) with v. anguillarum induced rapid synthesis and secretion of il-1beta, which initiates the innate immune response. snare proteins are known to regulate vesicular trafficking and fusion in all eukaryotes. here, we determined whether snare proteins, specifically vesicle-associated membrane protein-2 (vamp-2), are involved in regulated exocytosis of il-1beta of leu ... | 2006 | 16487935 |
| molecular characterization and expression analysis of stanniocalcin-1 in turbot (scophthalmus maximus). | stanniocalcin-1 (stc-1) is an anti-hypercalcemic hormone that is produced by the corpuscles of stannius (cs) in the teleost. the hormone is a homodimeric glycoprotein involved in the calcium and phosphate regulation in both teleost fish and mammals. in the present study, we have cloned and characterized a full-length cdna of stc-1 from the turbot (scophthalmus maximus) cs and examined its expression pattern in various tissues. the turbot stc cdna (1,246 nucleotides) encodes a putative preprohorm ... | 2006 | 16524573 |
| comparison of the growth and survival of larval turbot in the absence of culturable bacteria with those in the presence of vibrio anguillarum, vibrio alginolyticus, or a marine aeromonas sp. | larval turbot (scophthalmus maximus) were reared on rotifers (brachionus plicatilis) in the absence of culturable bacteria for up to 14 days and exhibited growth and high rates of survival (>55% in five experiments). low numbers of known bacteria were introduced into similar cultures by exposure of the rotifers to a suspension of bacteria prior to addition of rotifers to the larval cultures; vibrio anguillarum 91079 caused a highly significant decrease (p <0.01) in the proportion of survivors in ... | 1995 | 16535196 |
| genotyping and pathogenicity of viral hemorrhagic septicemia virus from free-living turbot (psetta maxima) in a turkish coastal area of the black sea. | viral hemorrhagic septicemia (vhs) is one of the most serious fish viral diseases for cultured rainbow trout (oncorhynchus mykiss), although vhs virus (vhsv) seems to be ubiquitous among marine fishes. in the present study, vhsv isolation was performed with free-living and cultured turbot (psetta maxima) in the trabzon coastal area of the black sea to evaluate participation of vhsv in mass mortalities of seed-produced turbot larvae. vhsv was detected in 14 of 66 free-living spawners (positive ra ... | 2006 | 16597932 |
| endocrine alteration in juvenile cod and turbot exposed to dispersed crude oil and alkylphenols. | juvenile atlantic cod (gadus morhua) and turbot (scophthalmus maximus) were exposed for 3 weeks in a continuous water flow to 0.5 ppm of dispersed north sea crude oil, 0.5 ppm of dispersed north sea crude oil spiked with 0.1 ppm of a mixture of alkylphenols (offshore oil production), and 30 ppb of nonylphenol (np). as potential markers of endocrine alteration, key enzymatic activities involved in both synthesis (17beta-hydroxysteroid dehydrogenases and p450 aromatase) and metabolism (liver udp-g ... | 2006 | 16600395 |
| investigation of micronuclei and other nuclear abnormalities in peripheral blood and kidney of marine fish treated with crude oil. | the induction of micronuclei and other nuclear abnormalities (nuclear buds, bi-nucleated and fragmented-apoptotic cells) was analyzed in the erythrocytes of peripheral blood and cephalic kidney of turbot (scophthalmus maximus) and atlantic cod (gadus morua), treated with crude oil (statfjord b, norway) and with nonylphenol. significant increase in mn was observed in turbot kidney and blood after exposure to 30 ppb of nonylphenol, 0.5 ppm of oil, and after co-exposure to 0.5 ppm of oil spiked wit ... | 2006 | 16603255 |
| comparison of protein expression in plasma from nonylphenol and bisphenol a-exposed atlantic cod (gadus morhua) and turbot (scophthalmus maximus) by use of seldi-tof. | the overall objective of this study was to compare the expression of plasma proteins in juvenile cod and turbot after a 3 week exposure to two different chemicals known to be estrogenic: 4-nonylphenol (np, 29 microg/l) and bisphenol a (bpa, 59 microg/l). proteinchip) array technology in combination with surfaced enhanced laser desorption ionisation-time of flight (seldi-tof) was used to investigate general responses in plasma proteins. in addition, an indirect enzyme-linked immunosorbent assay ( ... | 2006 | 16621060 |
| innate and adaptive immune responses of turbot, scophthalmus maximus (l.), following experimental infection with enteromyxum scophthalmi (myxosporea: myxozoa). | the innate and adaptive immune responses against enteromyxum scophthalmi was studied in turbot (scopthalmus maximus (l.)) experimentally exposed to the parasite by cohabitation. haematological, histopathological, cellular and humoral factors were determined in samples taken from control (ctrl) and recipient (rcpt, naïve fish cohabited with donor infected fish) animals at 0, 20, 29, 40 and 43 days post exposure (p.e). infection was first detected at day 20 p.e. and prevalence reached 100% at 40 d ... | 2006 | 16621605 |
| the beep stavanger workshop: mesocosm exposures. | within the beep project (biological effects of environmental pollution in marine ecosystems) the work package 1 was addressed to the development of new and more sensitive biomarkers of exposure in several sentinel organisms. within this framework, common mesocosm exposures of organic pollutants relevant for marine ecosystems were conducted in the facilities of akvamiljø a/s (stavanger, norway). in the first experiment, atlantic cod (gadus morhua), turbot (scophthalmus maximus) and shore crab (ca ... | 2006 | 16635530 |
| response of ig-positive cells to enteromyxum scophthalmi (myxozoa) experimental infection in turbot, scophthalmus maximus (l.): a histopathological and immunohistochemical study. | in recent years, a new parasite that causes severe losses has been detected in farmed turbot, scophthalmus maximus (l.), in northwestern spain. dead fish showed emaciation and cachexia caused by severe necrotizing enteritis that affected all portions of the digestive tract. the parasite was classified as a myxosporean and named enteromyxum scophthalmi. this study was set up to gain insights into the immune response of fish against this parasitic infection. the kinetics of immunoglobulin positive ... | 2006 | 16679029 |
| genotypic diversity of vibrio isolates associated with turbot (scophthalmus maximus) culture. | one hundred environmental strains of vibrio isolated from seawater and skin of healthy turbot in an aquaculture system were analyzed. chromosomal dna was digested with hindiii and mlui, and hybridized using a digoxigenin-labeled probe complementary to 16s and 23s rrna of escherichia coli. nineteen riboclusters were defined by ribotyping analysis at a value of sd> or = 70%, using the dice coefficient (s(d)) and upgma. the phylogenetic position of each ribocluster was achieved by 16s rrna gene seq ... | 2006 | 16730431 |
| comparison of the bioaccumulation from seawater and depuration of heavy metals and radionuclides in the spotted dogfish scyliorhinus canicula (chondrichthys) and the turbot psetta maxima (actinopterygii: teleostei). | the bioaccumulation of selected heavy metals and radionuclides ((241)am, (109)cd, (57)co, (51)cr, (134)cs, (54)mn and (65)zn) from seawater was experimentally compared in the chondrichthyan scyliorhinus canicula (spotted dogfish) and the actinopterygian teleost psetta maxima (turbot), of comparable size, age and benthic feeding habits. the speciation of these elements in seawater (salinity 38 per thousand, ph 8.1, temperature 16.5 degrees c) was also calculated to determine their potential bioav ... | 2006 | 16730782 |
| a challenge model for tenacibaculum maritimum infection in turbot, scophthalmus maximus (l.). | 2006 | 16768718 | |
| scrapie infectivity is quickly cleared in tissues of orally-infected farmed fish. | scrapie and bovine spongiform encephalopathy (bse) belongs to the group of animal transmissible spongiform encephalopathy (tse). bse epidemic in the uk and elsewhere in europe has been linked to the use of bovine meat and bone meals (mbm) in the feeding of cattle. there is concern that pigs, poultry and fish bred for human consumption and fed with infected mbm would eventually develop bse or carry residual infectivity without disease. although there has been no evidence of infection in these spe ... | 2006 | 16776828 |
| risk factors associated with enteromyxum scophthalmi (myxozoa) infection in cultured turbot, scophthalmus maximus (l.). | an epidemiological cohort study of enteromyxum scophthalmi in cultured turbot was performed on a farm in north western spain. four different ongrowing stocks (a, b, c, d) were monitored monthly until market size. fish from stocks c and d were divided into 2 subgroups, receiving filtered (cf and df) or unfiltered (cuf and duf) water. the lack of water filtration was positively associated with infection prevalence, as all fish kept in filtered water remained uninfected. parasite abundance varied s ... | 2006 | 16780606 |
| molecular identification, polymorphism, and expression analysis of major histocompatibility complex class iia and b genes of turbot (scophthalmus maximus). | major histocompatibility complex (mhc) class ii has a central role in the adaptive immune system by presenting foreign peptides to the t-cell receptor. the full lengths of mhc class ii a and b cdna were cloned from turbot by homology cloning and rapid amplification of cdna ends polymerase chain reaction (race pcr), and genomic organization, molecular polymorphism, and expression of turbot class iib gene were examined to study the function of class iib gene in fish. the deduced amino acid sequenc ... | 2006 | 16832747 |
| immunohistochemical study on the neuroendocrine system of the digestive tract of turbot, scophthalmus maximus (l.), infected by enteromyxum scophthalmi (myxozoa). | in recent years a new parasite, causing severe losses, has been detected in farmed turbot, scophthalmus maximus (l.), in northwestern spain. dead fish showed emaciation and cachexia caused by severe necrotizing enteritis, which affected all areas of the digestive tract. the parasite was classified as a myxosporean and named enteromyxum scophthalmi. this study was designed to assess the response of the turbot neuroendocrine system against e. scophthalmi infection. immunohistochemical tests were a ... | 2007 | 16844386 |
| molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (scophthalmus maximus). | antimicrobial peptides (amps) are regarded as important components of the host innate immune system and play crucial roles in host defence against microbial invasion. a small number of hepcidin amps have been isolated from teleosts. here, we report the isolation of a hepcidin gene from the liver of turbot (scophthalmus maximus) (genbank accession numbers: and ). in the 1037 bp-long genomic sequence, three exons and two introns were identified. the full-length cdna is 778bp long and contains an o ... | 2007 | 16908195 |
| identification of intestinal bacteria from japanese flounder (paralichthys olivaceus) and their ability to digest chitin. | the aim of the present study was to clarify the taxonomic status of intestinal bacteria isolated from japanese flounder (paralichthys olivaceus) and describe their ability to digest chitin. | 2006 | 16910942 |
| characterization of edwardsiella tarda strains isolated from turbot, psetta maxima (l.). | the biochemical, serological and molecular characteristics of a group of 21 edwardsiella tarda strains isolated from turbot, psetta maxima, in two different areas of europe were analysed and compared with a total of 13 strains of this bacterial species with different geographical and host origins. all the turbot isolates were biochemically identical to the e. tarda strains included as reference. the use of different techniques including microagglutination, dot blot and western blot of lipopolysa ... | 2006 | 16948704 |
| levamisole activates several innate immune factors in scophthalmus maximus (l.) teleostei. | levamisole, originally synthesized as an anti-helminthic, has been widely used in human and veterinary medicine as an immunomodulator or adjuvant. however, data on its use in fish are scarce, and no information is available for turbot. to study the effects of levamisole treatment on the innate immune system of juvenile turbot, two different doses (d1=500 mg/kg; d2=250 mg/kg dry food) were orally administered for 2 weeks and samplings were performed at -10, 14, 28, 49 and 77 days post treatment ( ... | 2006 | 17166395 |
| a single residue change in vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (scophthalmus maximus). | vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase b by gas chromatography. site-directed mutagenesis revealed that a specific residue, ser153, was critical for its enzymatic activity and for its virulence in fish. | 2007 | 17220231 |
| molecular cloning, characterization and expression analysis of natural resistance associated macrophage protein (nramp) cdna from turbot (scophthalmus maximus). | nramp (natural resistance associated macrophage protein) has been identified as one of the major candidate genes for controlling natural resistance and/or susceptibility to intracellular pathogens in vertebrates. however, few reports are available about the structure and function of nramp in teleost. we have recently isolated the cdna encoding nramp from turbot (scophthalmus maximus). the full-length cdna of the nramp is 2584 bp in length, including 69 bp 5' terminal utr, 850 bp 3' terminal utr ... | 2007 | 17317252 |
| establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (epinephelus akaara). | a marine fish cell line from the snout of red spotted grouper epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. the grouper snout cell line (gsc) cells multiplied well in dulbecco's modified eagle's medium (dmem) medium supplemented with 10% fetal bovine serum. the optimal growth temperature was 25 degrees c, and morphologically the cells were fibroblastic. chromosome analysis revealed that the gsc cell line has a normal ... | 2007 | 17342554 |
| isolation and characterization of scophthalmus maximus rhabdovirus. | a rhabdovirus associated with a lethal hemorrhagic disease in cultured turbot scophthalmus maximus linnaeus was isolated. the virus induced typical cytopathogenic effects (cpe) in 9 of 15 fish cell lines examined and was then propagated and isolated from infected carp leucocyte cells (clc). electron microscopy observations revealed that the negatively stained virions had a typical bullet-shaped morphology with one rounded end and one flat base end. the bullet-shaped morphology was more obvious a ... | 2007 | 17432038 |
| dietary uptake of dioxins (pcdd/pcdfs) and dioxin-like pcbs in spanish aquacultured turbot (psetta maxima). | the human population is exposed to dioxins (pcdd/fs) and dioxin-like polychlorinated biphenyls (dl-pcbs) mainly through diet; bioaccumulation and biomagnification in aquatic environment results in fishery products and by-products being an important vector to humans. the determination of pcdd/fs and dl-pcbs in fillets of young turbots (psetta maxima) (0-2 years) from aquaculture plant (galicia, spain) (n = 21) and in feeding stuffs were carried out, and dietary accumulation values and lipid-norma ... | 2007 | 17454116 |
| immunolocalization of metallothioneins in different tissues of turbot (scophthalmus maximus) exposed to cd. | metallothioneins (mt) were localized by immunochemistry in different organs and cell compartments of turbot exposed to sublethal concentrations (100 ppb) of cd for 7 days. the polyclonal rabbit anti-cod mt antibody (niva, norway) applied herein exhibited positive cross-reactivity with turbot mts. immunoreactive mts were localized in the branchial epithelium, in the liver and in the kidney of turbot. in cd exposed fishes mts were demonstrated mainly in branchial chloride cells (cc) and to a lesse ... | 2007 | 17455146 |
| scuticociliate infection and pathology in cultured turbot scophthalmus maximus from the north of portugal. | during the years 2004 and 2005 high mortalities in turbot scophthalmus maximus (l.) from a fish farm in the north of portugal were observed. moribund fish showed darkening of the ventral skin, reddening of the fin bases and distended abdominal cavities caused by the accumulation of ascitic fluid. ciliates were detected in fresh mounts from skin, gill and ascitic fluid. histological examination revealed hyperplasia and necrosis of the gills, epidermis, dermis and muscular tissue. an inflammatory ... | 2007 | 17465310 |
| experimental infection of turbot, psetta maxima (l.), with strains of viral haemorrhagic septicaemia virus isolated from wild and farmed marine fish. | the susceptibility of turbot, psetta maxima, to infection with two strains of viral haemorrhagic septicaemia virus (vhsv) obtained from wild greenland halibut, reinhardtius hippoglossoides, and from farmed turbot was examined. a marine vhsv strain known to be highly pathogenic for turbot was also utilized for comparative purposes. fish were infected by intra-peritoneal (i.p.), immersion or cohabitation, and maintained at two different temperatures (8 and 15 degrees c). infection trials showed th ... | 2007 | 17501740 |
| cloning and early expression pattern of two melatonin biosynthesis enzymes in the turbot (scophthalmus maximus). | melatonin biosynthesis from serotonin involves the sequential activation of the arylalkylamine n-acetyltransferase (aanat) and hydroxyindole-o-methyltransferase (hiomt). photoperiod synchronizes a daily rhythm in pineal and retinal melatonin secretion through controlling aanat activity. teleost fish possess two aanat, one expressed in the retina (aanat1) and the other expressed in the pineal gland (aanat2). we report here the full-length cloning of aanat1, aanat2, smhiomt and otx5 (orthodenticle ... | 2007 | 17561818 |
| protective acquired immunity to enteromyxum scophthalmi (myxozoa) is related to specific antibodies in psetta maxima (l.) (teleostei). | the acquired protection of three groups of turbot that had survived enteromyxosis outbreaks was tested by challenging with e. scophthalmi in three different experiments. the relation of such a response with the kinetics and duration of antibody production (determined by an elisa) was studied. the progression of the infection was evaluated by pcr. in experiments 1 and 2, in which turbot had cohabited with highly infected fish during outbreaks, parasite prevalence and mortality were very low or nu ... | 2007 | 17587343 |
| effects of medetomidine on hepatic erod activity in three species of fish. | medetomidine, an antifouling candidate, was investigated for its effects on cytochrome p4501a (cyp1a) activity in fish. rainbow trout (oncorhynchus mykiss), turbot (psetta maxima), and atlantic cod (gadus morhua) were exposed to medetomidine either via i.p. injection (<5 micromol (1mg)/kg) or via water (<50 nm). enzyme activity was measured as ethoxyresorufin-o-deethylase (erod) activity in liver microsomes. there was a small (2-7-fold) increase in erod activity in rainbow trout. in turbot, erod ... | 2008 | 17588660 |
| cloning, characterization and expression analysis of a novel cxc chemokine from turbot (scophthalmus maximus). | chemokines represent a superfamily of chemotactic cytokines playing an important role in leucocyte chemotaxis. here we report a novel turbot cxc chemokine screened from a turbot spleen cdna library. the complete cdna of the turbot cxc chemokine contains an 81bp 5' utr, a 414bp open reading frame (orf) encoding 137 amino acids and a 449bp 3' utr. four exons and three introns are identified in the turbot cxc chemokine gene. phylogenetic analysis showed that the turbot cxc chemokine clustered apart ... | 2007 | 17604647 |
| in vitro activity of the nonsteroidal anti-inflammatory drug indomethacin on a scuticociliate parasite of farmed turbot. | the scuticociliatosis produced by the endoparasite philasterides dicentarchi is a severe parasitic infection of farmed turbot (scophthalmus maximus) characterized by several histopathological effects including extensive inflammation. indomethacin is a nonsteroidal anti-inflammatory drug that specifically inhibits synthesis of the proinflammatory mediator prostaglandins. the effect of indomethacin on the in vitro growth of p. dicentrarchi was investigated. in vitro growth of the scuticociliate wa ... | 2007 | 17681428 |
| histopathology of experimental scuticociliatosis in turbot scophthalmus maximus. | a scuticociliate strain (b-2), originally isolated from an outbreak in a turbot scophthalmus maximus (= psetta maxima) farm in galicia (northwestern spain) and maintained in axenic culture, was injected intracoelomically (lethal dose 80 equivalent, ld80) in healthy turbot (50 g). ciliate-injected fish were kept under controlled conditions in a recirculating seawater system and sampled on days 1 through 8, 10, 12 and 14 postinfection (pi). necropsies were conducted and included blood collection f ... | 2007 | 17760386 |
| phenotypic, serological and genetic characterization of pseudomonas anguilliseptica strains isolated from cod, gadus morhua l., in northern europe. | the biochemical, serological and genetic characteristics of six strains of pseudomonas anguilliseptica isolated from cod, gadus morhua, in scotland were compared to well characterized isolates of this same bacterial species but of different origin. biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. similar results were obtained for the six cod isolates in the serological assays, a ... | 2007 | 17958609 |
| immune response of turbot, psetta maxima (l.) (pisces: teleostei), to formalin-killed scuticociliates (ciliophora) and adjuvanted formulations. | the increasing frequency of scuticociliatosis in turbot culture has stressed the need of knowledge on the immune responses to these parasites, for further developing of prevention and control strategies. the immune response of turbot to killed parasites, alone (ag) or in combination with montanide isa 763a (mon), was studied in a laboratory-scale experiment. the variations of several innate immune factors and the antibody response were analysed in immunized vs. non-immunized fish at different ti ... | 2008 | 17980620 |
| tenacibaculum discolor sp. nov. and tenacibaculum gallaicum sp. nov., isolated from sole (solea senegalensis) and turbot (psetta maxima) culture systems. | two gram-negative, rod-shaped, gliding bacterial strains, designated strain ll04 11.1.1(t) and strain a37.1(t), were isolated from a diseased sole (solea senegalensis) and from seawater from a holding tank for turbot (psetta maxima), respectively. the strains grew on solid media as bright yellow colonies with uneven edges; the colonies did not adhere to the agar. the bacteria were able to grow at temperatures in the range 14 to 38 degrees c and from ph 6.0 to ph 8.0. the dna g+c contents of stra ... | 2008 | 18175676 |
| detection of carbohydrate terminals in the enteric parasite enteromyxum scophthalmi (myxozoa) and possible interactions with its fish host psetta maxima. | the existence and localisation of carbohydrate terminals in enteromyxum scophthalmi stages was investigated at light (lm) and transmission electron microscopes (tem) using lectin histochemistry techniques, with the aim of contributing to elucidate the participation of carbohydrate-lectin interactions in the parasite invasion and relationships with the fish host. the presence of abundant mannose and/or glucose residues was demonstrated by the intense staining by concanavalin a at both lm and tem. ... | 2008 | 18311571 |
| isolation of an unusual strain of edwardsiella tarda from turbot and establish a pcr detection technique with the gyrb gene. | the aim of this study was to report an unusual edwardsiella tarda and develop an effective method to identify this bacterium. | 2008 | 18341558 |
| development and characterization of monoclonal antibodies to spring viraemia of carp virus. | five monoclonal antibodies (mabs) against spring viraemia of carp (svcv0504, isolated from common carp in china) were produced from mice immunized with purified virus preparations. the virion of svcv contains five structural proteins, representing the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). western blotting analysis revealed that three mabs (1h5, 1e10, and 1h7) recognized specifically to a single protein of 47kda (n), the ... | 2008 | 18378003 |
| distribution of small plasmids in aeromonas salmonicida subsp. salmonicida strains isolated from nw spain and portugal: evidence of clonality in strains isolated from turbot, psetta maxima (l.). | 2008 | 18471103 | |
| detection of cutaneous antibodies in excised skin explants from grass carp, ctenopharyngodon idella (valenciennes), immune to scophthalmus maximus rhabdovirus. | this study determined whether cutaneous antibodies were present in excised skin explants of grass carp, ctenopharyngodon idella, immune to scophthalmus maximus rhabdovirus (smrv). culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (ielisa), western blot, indirect immunofluorescent assay (ifa) and flow cytometry (fcm). ielisa showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized ... | 2008 | 18482386 |
| environmental hypoxia as a metabolic constraint on fish: the case of atlantic cod, gadus morhua. | hypoxia is known to provoke a wide range of effects on aquatic animals. here we use laboratory and field data on atlantic cod, gadus morhua, to illustrate that many of these responses can be explained within the metabolic scope (ms) framework, i.e. taking into account the directive and limiting effects of dissolved oxygen (do) on the ability of animals to acquire energy for growth and activity. a ms model for cod shows that scope for activity (swimming, feeding, etc.) is proportional to do and b ... | 2008 | 18508091 |
| genotoxic evaluation of the gh transgenic synechocystis using mice and turbot (scophthalmus maximus l.). | in the present paper, the genotoxicity of transgenic synechocystis sp. pcc 6803 containing flounder (paralichthys olivaceus) growth hormone gene has been assessed using micronucleus tests in mice and fish. no micronucleus inductions in bone marrow erythrocyte of mice were observed at three doses of transgenic synechocystis (2.0, 5.0, and 10.0g/kg). the frequencies of micronucleus and other nuclear abnormalities in turbot (scophthalmus maximus l.) fed with 0.2%, 0.5% and 1.0% transgenic synechocy ... | 2008 | 18515177 |
| identification of immune-related genes from kidney and spleen of turbot, psetta maxima (l.), by suppression subtractive hybridization following challenge with vibrio harveyi. | suppression subtractive hybridization was used to investigate the response of turbot, psetta maxima (l.), to vibrio harveyi, by using a cdna library constructed from artificially infected turbot kidney and spleen mrna. forty-nine expressed sequence tags were obtained. several immune system genes were identified, including a major histocompatibility complex (mhc) class ia gene and a heat shock protein 70 gene. some signalling molecules were also present in the cdna libraries, including src-family ... | 2008 | 18577100 |
| severe scuticociliate (philasterides dicentrarchi) infection in a population of sea dragons (phycodurus eques and phyllopteryx taeniolatus). | scuticociliatosis is a disease of fish induced by ciliated parasites of the genus scuticociliatida. it has been described in sea horses (hippocampus sp.), flounders (paralichthys olivaceus), and turbots (scophthalmus maximus). here we present a case study of a population of sea dragons chronically infected with scuticociliates identified as philasterides dicentrarchi by histopathology and pcr. beginning in 2004, over a period of 19 months, 10 sea dragons (phycodurus eques and phyllopteryx taenio ... | 2008 | 18587104 |
| development of an effective edwardsiella tarda vaccine for cultured turbot (scophthalmus maximus). | since 2004 edwardsiella tarda has become one of the most important emerging pathogens in turbot aquaculture industry in europe causing serious economic losses. therefore, this study aimed to design an effective vaccination strategy to prevent edwardsiellosis in this fish species. two vaccine formulations, an adjuvanted vaccine and an aqueous bacterin, and different routes of administration, bath and intraperitoneal injection (i.p.), were tested. the effectiveness of the different immunization st ... | 2008 | 18602276 |
| living together: the marine amoeba thecamoeba hilla schaeffer, 1926 and its endosymbiont labyrinthula sp. | two protists isolated simultaneously from the same sample of gill tissue of psetta maxima (l.) were identified as thecamoeba hilla schaeffer, 1926 and labyrinthula sp. a labyrinthula strain (lth) derived from a mixed culture of both organisms was well established in a short time, while subcultures of t. hilla continued to be associated with labyrinthula cells despite all efforts to eliminate them. ultrastructural examination, repeated several times in the course of long-lasting subculturing of a ... | 2008 | 18619822 |
| detection of quorum-sensing-related molecules in vibrio scophthalmi. | cell-to-cell communication (also referred to as quorum sensing) based on n-acyl-homoserine lactones (ahls) is a widespread response to environmental change in gram-negative bacteria. ahls seem to be highly variable, both in terms of the acyl chain length and in the chemical structure of the radicals. another quorum sensing pathway, the autoinducer-2-based system, is present both in gram-positive and gram-negative bacteria. in this study the presence of signal molecules belonging to both quorum s ... | 2008 | 18700048 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| expressed sequence tags (ests) from immune tissues of turbot (scophthalmus maximus) challenged with pathogens. | the turbot (scophthalmus maximus; scophthalmidae; pleuronectiformes) is a flatfish species of great relevance for marine aquaculture in europe. in contrast to other cultured flatfish, very few genomic resources are available in this species. aeromonas salmonicida and philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in ... | 2008 | 18817567 |
| molecular cloning, characterization and expression analysis of cathepsin d gene from turbot scophthalmus maximus. | cathepsin d is a lysosomal endoproteolytic aspartic proteinase which also has been found in endosomes of macrophage. it is thought to play key roles in the developmental and physiological process of animals. the est sequence of turbot (scophthalmus maximus l.) cathepsin d was obtained from a subtractive cdna library. in the present study, 5'-race and 3'-race were carried out to obtain the complete cdna sequence of turbot cathepsin d, which contained a 91 bp 5'-utr, a 1191 bp open reading frame e ... | 2009 | 18948209 |
| phaeobacter and ruegeria species of the roseobacter clade colonize separate niches in a danish turbot (scophthalmus maximus)-rearing farm and antagonize vibrio anguillarum under different growth conditions. | members of the roseobacter clade colonize a spanish turbot larval unit, and one isolate (phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. here, we demonstrate that roseobacters with antagonistic activity against vibrio anguillarum also colonize a danish turbot larval farm that relies on a very different water source (the danish fiord limfjorden as opposed to the galician atlantic ocean). phylogenetic analyses based on 16s rrna and gyrase b gene sequences re ... | 2008 | 18952864 |
| response of mucosal and systemic sigm-positive cells in turbot (scophthalmus maximus l.) immunization with edwardsiella tarda. | indirect fluorescence analyze technology (ifat) and fluorescence-activated cell sorter (facs) analysis were used to detect surface igm positive cells (sigm+ cells) in lymphoid tissues of unvaccinated turbots and vaccinated ones with inactivated edwardsiella tarda. the results were as follows: (1) the percentage of sigm+ cells respectively was 0+/-0.00% of skin, 1.58+/-0.40% of gills, 17.05+/-0.39% of peripheral blood (pbl), 21.06+/-1.79% of kidney in unvaccinated healthy turbots (average length: ... | 2009 | 19178953 |
| real-time pcr detection and quantification of fish probiotic phaeobacter strain 27-4 and fish pathogenic vibrio in microalgae, rotifer, artemia and first feeding turbot (psetta maxima) larvae. | to develop a sybr green quantitative real-time pcr protocol enabling detection and quantification of a fish probiotic and two turbot pathogenic vibrio spp. in microcosms. | 2009 | 19187159 |
| rapid diagnosis of turbot reddish body iridovirus in turbot using the loop-mediated isothermal amplification method. | turbot reddish body iridovirus (trbiv) is a new piscine iridovirus that infects the turbot, scophthalmus maximus, cultured in northern china and can cause high mortality. in this study, a loop-mediated isothermal amplification (lamp) method was developed for the specific detection of this virus using primers designed from an msp i restriction dna fragment of the trbiv genome. mg(2+) concentrations, the reaction temperature, and the reaction time of lamp were optimized to 6mm, 65 degrees c, and 6 ... | 2009 | 19187785 |
| resveratrol promotes an inhibitory effect on the turbot scuticociliate parasite philasterides dicentrarchi by mechanisms related to cellular detoxification. | the mechanisms involved in antiparasitic activity of the natural nonflavonoid polyphenol resveratrol (resv) on the turbot (psetta maxima) scuticoliate parasite philasterides dicentarchi were investigated. at concentrations higher than 50microm, resv caused significant inhibition of the in vitro growth of the ciliates, which was apparent on the third day of culture and, at the same concentration, resv caused significant inhibition of o(2) consumption. resv, at a concentration of 100microm, produc ... | 2009 | 19246159 |
| characterization of edwardsiella tarda rpos: effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. | in this study, rpos gene was identified from edwardsiella tarda eib202 and its functional role was analyzed by using an in-frame deletion mutant rpos and the complemental strain rpos (+). compared with the wild type and rpos (+), rpos was impaired in terms of the ability to survive under oxidative stress and nutrient starvation, as well as the resistance to 50% serum of scophthalmus maximus in 3 h, demonstrating essential roles of rpos in stress adaptation. the rpos mutant also displayed markedl ... | 2009 | 19283379 |
| molecular characterizations of chloramphenicol- and oxytetracycline-resistant bacteria and resistance genes in mariculture waters of china. | in order to gain an understanding of the diversity and distribution of antimicrobial-resistant bacteria and their resistance genes in maricultural environments, multidrug-resistant bacteria were screened for the rearing waters from a mariculture farm of china. both abalone haliotis discushannai and turbot scophthalmus maximus rearing waters were populated with abundant chloramphenicol-resistant bacteria. these bacteria were also multidrug resistant, with vibriosplendidus and vibriotasmaniensis b ... | 2009 | 19303610 |
| cdna cloning, characterization, and expression analysis of the rac1 gene from scophthalmus maximus. | rac1 is a small gtp-binding protein that belongs to the rho small gtpases, which are important signaling molecules that regulate the dynamics of the actin cytoskeleton and mediate changes in cell morphology and motility. the est sequence of rac1 from turbot (scophthalmus maximus l.) was obtained from a subtractive cdna library previously. in this study, the full-length cdna sequence of turbot rac1 was obtained, which was 2420 nucleotides (nt) encoding a protein of 192 amino acids, with a putativ ... | 2009 | 19426825 |
| molecular cloning and tissue expression patterns of a small conductance calcium-activated potassium channel gene in turbot (scophthalmus maximus l.). | calcium-activated potassium channels on plasma membrane enable potassium influx into the cell with ensuing changes in plasma membrane potential and consequent effects on cellular metabolic functions. recently, this potassium channel was reported to regulate the cellular responses of mammalian immune cells. we have postulated the presence of such a channel in fish immune cells and its potential role in immunoregulation in fish. employing specific primers and rna template, we cloned a segment of a ... | 2009 | 19481607 |
| specific mhc class ii b alleles associated with resistance to edwardsiella tarda in turbot, psetta maxima (l.). | 2009 | 19486240 | |
| detection of different quorum-sensing signal molecules in a virulent edwardsiella tarda strain ltb-4. | the aim of this study was to elucidate the potential quorum-sensing (qs) signal molecules of an emerging pathogen (edwardsiella tarda strain ltb-4) of cultured turbot (scophthalmus maximus). | 2010 | 19548884 |
| dominance of dietary intake of metals in marine elasmobranch and teleost fish. | metal accumulation in marine fish is a global public health concern, because the consumption of seafood provides the largest dose of many toxic metals to humans. while water quality criteria often rely on aqueous exposures of metals to fish in developing safety guidelines, it is increasingly recognized that marine fish obtain an important fraction of their metal body burden from their diet. using experimental data, we modeled the accumulation of six metals (am, cd, cs, co, mn, zn) from diet and ... | 2009 | 19580992 |
| host and environmental risk factors associated with cryptosporidium scophthalmi (apicomplexa) infection in cultured turbot, psetta maxima (l.) (pisces, teleostei). | an epidemiological cohort study of cryptosporidium scophthalmi in cultured turbot psetta maxima l. of northwestern spain was conducted along a four-year period. four different ongrowing cohorts were monitored monthly from introduction into the ongrowing tanks (10-50 g) until reaching market size (400-1400 g). the association of host and environmental factors with five categories of parasite abundance was assessed using a multivariable regression framework. epidemiological factors assessed here w ... | 2009 | 19674844 |
| experimental pseudomonas anguilliseptica infection in turbot psetta maxima (l.): a histopathological and immunohistochemical study. | experimental infection with pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (psetta maxima) in order to evaluate the pathology induced. turbot was found to be sensitive to i.p. challenge (1.7x10(6) cfu/fish) but no to bath exposure. the i.p. challenge induced septicaemic infection and mortality. externally, moribund fish showed distended abdomen and pale areas at day 9. the gross pathological internal signs present were abundant ascitic ... | 2009 | 19683980 |
| vitellogenin of the chilean flounder paralichthys adspersus as a biomarker of endocrine disruption along the marine coast of the south pacific. part i: induction, purification, and identification. | we sought to provide a useful indicator of the presence of endocrine-disrupting contaminants along the marine coast of the south pacific using chilean flounder (paralichthys adspersus). in light of the lack of information on vitellogenin for this species, we induced, purified, and identified the plasma vitellogenin of chilean flounder inhabiting the chilean coast. vitellogenin (vg) from chilean flounder was purified by size exclusion and ion-exchange chromatography using plasma from juvenile mal ... | 2010 | 19685217 |
| bioremediation of bacteria pollution using the marine sponge hymeniacidon perlevis in the intensive mariculture water system of turbot scophthalmus maximus. | sessile filter-feeding marine sponges (porifera) have been reported to possess high efficiency in removing bacteria pollution from natural or aquaculture seawater. however, no investigation has been carried out thus far in a true mariculture farm water system. therefore this study sought to investigate the ability of the marine sponge hymeniacidon perlevis to bioremediate the bacteria pollution in the intensive aquaculture water system of turbot scophthalmus maximus. sponge specimens were hung i ... | 2010 | 19725041 |
| molecular cloning, characterization, and expression analysis of the cxcr4 gene from turbot: scophthalmus maximus. | chemokine receptor 4 (cxcr4) belongs to the large superfamily of g protein-coupled receptors. the est sequence of cxcr4 from turbot (scophthalmus maximus l.) was obtained from a subtractive cdna library. in the present study, the full-length cdna sequence of turbot cxcr4 was obtained, and sequence analysis indicated that its primary structure was highly similar to cxcr4 from other vertebrates. quantitative real-time pcr demonstrated that the highest expression level of turbot cxcr4 was in the sp ... | 2009 | 19750236 |
| design and performance of a turbot (scophthalmus maximus) oligo-microarray based on ests from immune tissues. | an expressed sequence tag database from immune tissues was used to design the first high-density turbot (scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. specific oligonucleotides (60 mers) were successfully designed for 2,716 out of 3,482 unique sequences of the database. an agilent custom oligo-microarray 8 x 15 k (five replicates/gene; eight microarrays/slide) was constructed. the performance of the microarray and the sources of va ... | 2010 | 19844759 |
| detection of quorum sensing signal molecules and mutation of luxs gene in vibrio ichthyoenteri. | some pathogenic species belonging to the vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. in this study, three kinds of n-acylhomoserine lactone (ahl) signal molecules were detected in vibrio ichthyoenteri da3, a pathogen of cultured turbot (scophthalmus maximus), with the agrobacterium tumefaciens reporter strain kyc55 (pjz372)(pjz384)(pjz410). da3 produced ahls during the entire growth phase, and the level of ahls increased, acc ... | 2010 | 19878720 |
| antibody responses of turbot psetta maxima against various antigen formulations of scuticociliates ciliophora. | the kinetics of the antibody production and the protection at challenge were studied in turbot inoculated with various scuticociliate antigen preparations: live ciliates putatively attenuated through long-term in vitro culture (trial 1) and formalin-killed ciliates without or with gerbu adjuvant in trials 2, 3, and 4. antigen used in killed preparations was a mixture of 3 different ciliate isolates (v3) in the case of trials 2 and 3, whereas in trial 4, monovalent (v1), trivalent (v3) or pentava ... | 2009 | 19902841 |
| zschokkella hildae auerbach, 1910: phylogenetic position, morphology, and location in cultured atlantic cod. | the myxozoan zschokkella hildae auerbach, 1910, was detected with a prevalence of 100% in cultured atlantic cod, gadus morhua l. aged 1+ from a culture facility on the west coast of scotland. sporogonic stages of z. hildae, plasmodia producing 2-5 mature spores, were located predominantly in the collecting ducts and ureters of the kidney, and spores were present in the urine collected from the bladder. less frequently, plasmodia were detected in the interstitial tissue of the kidney. the parasit ... | 2010 | 20026425 |
| transmission of lungworms of harbour porpoises and harbour seals: molecular tools determine potential vertebrate intermediate hosts. | harbour porpoises (phocoena phocoena) and harbour seals (phoca vitulina) from german waters are infected by six species of lungworms (metastrongyloidea). these nematodes parasitise the respiratory tract, are pathogenic and often cause secondary bacterial infections. in spite of their clinical and epidemiological significance, the life cycle and biology of lungworms in the marine environment is still largely unknown. regions of ribosomal dna (its-2) of all lungworms parasitising harbour porpoises ... | 2010 | 20123100 |
| cloning and analysis of a ferritin subunit from turbot (scophthalmus maximus). | ferritin is an evolutionarily conserved protein that plays an important role in iron storage and detoxification. in this study, the gene encoding a ferritin h subunit homologue (smfer1) was cloned from turbot (scophthalmus maximus) and analyzed at the expression and functional levels. the open reading frame of smfer1 is 534 bp and preceded by a 5'-untranslated region that contains a putative iron regulatory element (ire). the deduced amino acid sequence of smfer1 shares extensive sequence identi ... | 2010 | 20152905 |
| ribosomal rna gene sequences confirm that protistan endoparasite of larval cod gadus morhua is ichthyodinium sp. | an enigmatic protistan endoparasite found in eggs and larvae of cod gadus morhua and turbot psetta maxima was isolated from baltic cod larvae, and dna was extracted for sequencing of the parasite's small subunit ribosomal rna (ssu rrna) gene. the endoparasite has previously been suggested to be related to ichthyodinium chabelardi, a dinoflagellate-like protist that parasitizes yolk sacs of embryos and larvae of a variety of fish species. comparison of a 1535 bp long fragment of the ssu rrna gene ... | 2010 | 20225677 |
| identification and molecular analysis of a novel c-type lectin from scophthalmus maximus. | c-type lectins are calcium-dependent carbohydrate-binding proteins that play important roles in innate immunity. in this study, a c-type lectin homologue (smlec1) was identified from turbot (scophthalmus maximus) and analyzed at expression and functional levels. the open reading frame of smlec1 is 504 bp, with a 5'-untranslated region (utr) of 101 bp and a 3'-utr of 164 bp. the deduced amino acid sequence of smlec1 shares 34%-38% overall identities with the c-type lectins of several fish species ... | 2010 | 20226863 |
| identification and analysis of a scophthalmus maximus ferritin that is regulated at transcription level by oxidative stress and bacterial infection. | ferritins are conserved iron storage proteins that exist in most living organisms and play an essential role in iron homeostasis. in this study, we reported the identification and analysis a ferritin m subunit, smferm, from turbot scophthalmus maximus. the full length cdna of smferm contains a 5'-untranslated region (utr) of 232 bp, an open reading frame (orf) of 531 bp, and a 3'-utr of 196 bp. the orf encodes a putative protein of 176 amino acids, which shares extensive sequence identities with ... | 2010 | 20382253 |
| comparative susceptibility of turbot, halibut, and cod yolk-sac larvae to challenge with vibrio spp. | in intensive aquaculture systems, high mortalities are frequently observed during the early life stages of marine fish. the aim of this study was to investigate differences in the susceptibility of turbot scophthalmus maximus, halibut hippoglossus hippoglossus and cod gadus morhua to various strains of vibrio anguillarum (serotypes o1, o2alpha and o2beta), v salmonicida and v splendidus. the bath challenge experiments were performed using a multidish system, with 1 egg well-1. unchallenged eggs ... | 2010 | 20391910 |
| strains of uronema marinum (scuticociliatia) co-isolated with amoebae of the genus neoparamoeba. | mixed infections of histophagous ciliates and neoparamoeba spp. page, 1987 were diagnosed in gill tissue of farmed turbot psetta maxima (synonym: scophthalmus maximus) and atlantic salmon salmo salar during a study of amoebic gill disease. ciliates co-isolated from lesions grossly visible on gills of both fish hosts and from 2 species of red algae lithophyllum racemus and palmaria palmata were characterized morphologically and by using molecular markers. sequences of small subunit (ssu) rdna wer ... | 2010 | 20391914 |
| identification and analysis of a cpg motif that protects turbot (scophthalmus maximus) against bacterial challenge and enhances vaccine-induced specific immunity. | oligodeoxynucleotides (odns) containing unmethylated cpg motifs in certain contexts are known to be immunostimulatory in vertebrate systems. cpg odns with immune effects have been identified for many fish species but, to our knowledge, not for turbot. in this study, a turbot-effective cpg odn, odn 205, was identified and a plasmid, pcn5, was constructed which contains the cpg motif of odn 205. when administered into turbot via intraperitoneal (i.p.) injection, both odn 205 and pcn5 could (i) inh ... | 2010 | 20416262 |
| analysis of the expression and antioxidative property of a peroxiredoxin 6 from scophthalmus maximus. | peroxiredoxins (prxs) are a group of antioxidant proteins that protect cells from oxidative damage caused by various peroxides. to date, six different isoforms of peroxiredoxin (prx1 to prx6) have been identified, of which, prx6 belongs to the 1-cys prx subfamily. although prx6 of several fish species have been reported at sequence level, there are very few documented studies on the potential function of fish prx6. in this report, we describe the identification and analysis of a prx6 homologue, ... | 2010 | 20420920 |
| phylogenetic consistencies among chondrichthyan and teleost fishes in their bioaccumulation of multiple trace elements from seawater. | multi-tracer experiments determined the accumulation from seawater of selected radioactive trace elements (mn-54, co-60, zn-65, cs-134, am-241, cd-109, ag-110m, se-75 and cr-51) by three teleost and three chondrichthyan fish species to test the hypothesis that these phylogenetic groups have different bioaccumulation characteristics, based on previously established contrasts between the carcharhiniform chondrichthyan scyliorhinus canicula (dogfish) and the pleuronectiform teleost psetta maxima (t ... | 2010 | 20471062 |
| light and electron microscopic studies on turbot psetta maxima infected with enteromyxum scophthalmi: histopathology of turbot enteromyxosis. | in the last decade, a new parasite that causes severe losses has been detected in farmed turbot psetta maxima (l.), in north-western spain. the parasite was classified as a myxosporean and named enteromyxum scophthalmi. the aim of this study was to characterize the main histological changes that occur in e. scophthalmi-infected turbot. the parasite provoked catarrhal enteritis, and the intensity of the lesions was correlated with the progression of the infection and with the development of the p ... | 2010 | 20481088 |
| development and characterization of a new marine fish cell line from turbot (scophthalmus maximus). | a new marine fish cell line, tk, derived from turbot (scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. the tk cells were maintained in minimum essential medium eagle (mem) supplemented with hepes, antibiotics, fetal bovine serum (fbs), 2-mercaptoethanol (2-me), and basic fibroblast growth factor (bfgf). the suitable growth temperature for tk cells was 24°c, and microscopically, tk cells were comp ... | 2010 | 20496112 |
| establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus. | a turbot, scophthalmus maximus, fin (tf) cell line was established and susceptibility to turbot reddish body iridovirus (trbiv) was determined in this study. primary culture of tf cells was initiated from fin tissue pieces partially digested with trypsin, collagenase ii and hyaluronidase. digested tissue pieces were cultured at 24 degrees c in leibovitz-15 medium (ph 7.2), supplemented with 20% fetal bovine serum, carboxymethyl chitosan, n-acetylglucosamine hydrochloride, basic fibroblast growth ... | 2010 | 20502962 |