Publications

evaluation of the genetic diversity of bupleurum using amplified fragment length polymorphism analysis.	radix bupleuri (chaihu), the dried root of the bupleurum plant, is an important component of traditional chinese medicine. in this study, we examined the genetic diversity of 11 bupleurum strains, originating from 7 provinces in china, using amplified fragment length polymorphism analysis. a total of 274 polymorphic bands were obtained using 6 primer combinations, indicating a high level of polymorphism across all strains. an estimation of the relative relationships among strains revealed geneti ...	2015	25867406
prescription of chinese herbal medicine in pattern-based traditional chinese medicine treatment for depression: a systematic review.	traditional chinese medicine (tcm) treatments are often prescribed based on individuals' pattern diagnoses. a systematic review of randomized controlled trials in chinese and english literatures on tcm pattern-based treatment for depression has therefore been conducted. a total of 61 studies, 2504 subjects, and 27 tcm patterns were included. due to the large variation of tcm pattern among participants, we only analyzed the top four commonly studied tcm patterns: liver qi depression, liver depres ...	2015	26180532
cytotoxic triterpenoid glycosides (saikosaponins) from the roots of bupleurum chinense.	as a part of our ongoing studies on cytotoxic triterpenoid saponins from herbal medicines, phytochemical investigation of the roots of bupleurum chinense dc. afforded four new saikosaponins (1-4), along with 16 known ones (5-20). their structures were established by direct interpretation of their spectral data, mainly hr-esi-ms, 1d nmr and 2d nmr, and by comparison with literature data. among them, compound 20 was isolated from the natural product for the first time. the cytotoxicities of all co ...	2015	26259802
antioxidant, anticancer and apoptotic effects of the bupleurum chinense root extract in ho-8910 ovarian cancer cells.	the purpose of this study was to evaluate the anticancer, apoptotic and antioxidant properties of bupleurum chinense (b.c) root extract against human epithelial ovarian cancer cells (ho-8910) in vitro.	2015	26537084
[clinical observation of breast hyperplasia treated with auricular point sticking therapy and xiaopijian].	to observe the efficacy difference in the treatment of breast hyperplasia between the combined therapy of auricular point sticking and xiaopijian and the simple application of xiaopijian.	2015	26571890
development of a c3c-based elisa method for the determination of anti-complementary potency of bupleurum polysaccharides.	traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. however, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. the main objective of our study was to develop a specific enzyme-linked immunosorbent assay (elisa) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. by using ...	2015	26579461
anti-melanoma activity of bupleurum chinense, bupleurum kaoi and nanoparticle formulation of their major bioactive compound saikosaponin-d.	bupleurum chinense is a traditional chinese medicinal herb which has been used to treat various inflammatory and infectious diseases, while bupleurum kaoi is an endemic plant in taiwan. we determined whether b. chinense and b. kaoi and their biologically active saikosaponin compounds possess anti-melanoma activity. in addition, we developed a novel saikosaponin-d nanoparticle system to improve its solubility, and evaluated its antiproliferative effects and molecular mechanisms in melanoma cells.	2016	26748071
neuroprotective oleanane triterpenes from the roots of bupleurum chinense.	the discovery of new natural compounds with pharmacological properties is an increasingly important field, and a continuous phytochemical investigation of the roots of bupleurum chinense d.c. has led to the isolation of 17 triterpenoids, including three new oleanane triterpenes (1-3) together with 14 known ones. their structures were determined on the basis of 1d and 2d nmr spectra as well as hr-esi-ms data. the cytotoxicities of all compounds against five selected human cancer cell lines were a ...	2016	26883148
[evaluation for merchandise character and quality of bupleurum chinense root from different habitats].	to evaluate the merchandise character and quality of bupleurum chinense root from different habitats.	2015	26983230
vinegar amount in the process affected the components of vinegar-baked radix bupleuri and its hepatoprotective effect.	bupleuri radix (in chinese chaihu), the dried roots of bupleurum chinense dc, is a traditional chinese medicine widely used to treat fever, hepatitis, jaundice, nephritis, dizziness. when baked with vinegar, its effect is more focused on liver related disease. this paper was undertaken to determine the best vinegar amount in the processing and explore its key efficacy components.	2016	27599678
antioxidant and cytotoxic lignans from the roots of bupleurum chinense.	in the search for biologically active compounds from the roots of bupleurum chinense d c., phytochemical investigation of its ethanol extract led to the isolation and identification of a new 8-o-4' neolignan glucoside, saikolignanoside a (1), along with eight known lignans (2-9). their structures were determined on the basis of ir, uv, hresims, and nmr spectroscopic analyses. the antioxidant and cytotoxic effects of isolated compounds were evaluated in vitro. the isolated compounds (ic50 > 200 μ ...	2017	27649745
toxic polyacetylenes in the genus bupleurum (apiaceae) - distribution, toxicity, molecular mechanism and analysis.	the genus bupleurum includes approximately 200 species that are widely distributed in the northern hemisphere, eurasia and north africa. certain species of this genus have long been used as antiphlogistic, antipyretic and analgesic agents in traditional folk medicine. as described in the chinese pharmacopoeia, the roots of bupleurum chinense dc. and b. scorzonerifolium willd. are the herbal materials that compose chaihu (radix bupleuri), a well-known tcm herb.	2016	27693772
bupleurum chinense polysaccharide inhibit adhesion of human melanoma cells via blocking β1 integrin function.	adhesive interaction contributes toward tumor metastasis and the transmembrane glycoprotein receptor, integrin has been recognized to mediate the adhesion to extracellular matrix thus upregulating tumor metastasis. in the current study, we evaluated the anti-adhesive mechanisms of a water-soluble polysaccharide (bcp) extracted from bupleurum chinense. bcp inhibited integrin-mediated adhesion of human melanoma a375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine. ...	2017	27842819
anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide bcap-1 from bupleurum chinense via nf-κb signaling pathway.	bupleurum chinense is a well-known traditional chinese medicine. polysaccharides extracted from medical plants possess multiple healthy benefits. in the present study, an alkali-extracted polysaccharide (bcap-1) was isolated from bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. bcap-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by deae-cellulose and sepharose cl-6b columns. bcap-1 markedly inhibite ...	2017	27884671