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development of a pcr-based line probe assay for identification of fungal pathogens.we report on a reverse-hybridization line probe assay (lipa) which when combined with pcr amplification detects and identifies clinically significant fungal pathogens including candida, aspergillus, and cryptococcus species. dna probes have been designed from the internal transcribed-spacer (its) regions of candida albicans, candida parapsilosis, candida glabrata, candida tropicalis, candida krusei, candida dubliniensis, cryptococcus neoformans, aspergillus fumigatus, aspergillus versicolor, asp ...200011015393
induction of cytotoxicity and production of inflammatory mediators in raw264.7 macrophages by spores grown on six different plasterboards.dampness and microbial growth in buildings are associated with respiratory symptoms in the occupants, but details of the phenomenon are not sufficiently understood. the current study examined the effects of growth conditions provided by six plasterboards on cytotoxicity and inflammatory potential of the spores of streptomyces californicus, penicillium spinulosum, aspergillus versicolor, and stachybotrys chartarum. the microbes were isolated from mold problem buildings and thereafter grown on six ...200111295859
allergic respiratory disease and fungal remediation in a building in a subtropical climate.an outbreak of allergic respiratory disease occurred in a new building that was characterized from initial occupancy by the presence of extensive visible mold (especially aspergillus versicolor) on interior surfaces. epidemiological study of the occupants of both the affected building and a comparison neighboring structure indicated high rates of respiratory and other symptoms among persons working in the affected building. subsequent clinical evaluations of some persons occupying the building f ...200111297052
aspergillus versicolor infection of the external auditory canal successfully treated with terbinafine. 200111306840
identification of aspergillus fumigatus and related species by nested pcr targeting ribosomal dna internal transcribed spacer regions.aspergillus fumigatus is the most common species that causes invasive aspergillosis. in order to identify a. fumigatus, partial ribosomal dna (rdna) from two to six strains of five different aspergillus species was sequenced. by comparing sequence data from genbank, we designed specific primer pairs targeting rdna internal transcribed spacer (its) regions of a. fumigatus. a nested pcr method for identification of other a. fumigatus-related species was established by using the primers. to evaluat ...200111376067
fungal flora on board the mir-space station, identification by morphological features and ribosomal dna sequences.this report is on the morphological and molecular biological identification, using 18s- and its1-rdna sequences, of the "space fungi" isolated on board the russian mir-space station as the major constituents of the fungal flora. the six fungal strains were isolated from air by using an air sampler or from condensation. strains were identified as penicillium chrysogenum, aspergillus versicolor, or penicillium sp. by both methods. the species of space fungi were common saprophytic fungi in our liv ...200111471823
air sampling of fungal spores on filters. an investigation on passive sampling and viability.in this study, glycerol was tested as a collection substrate for passive bioaerosol sampling. filters (mixed cellulose acetate and nitrate) were soaked in glycerol and exposed for an aerosol from three different fungal species: penicillum commune, aspergillus versicolor and paecilomyces variotii. the passive sampling method was compared with a closed-face polycarbonate filter sampling method. exposure was performed in an exposure chamber. the total number of spores was determined by microscopic ...199911529137
lipid peroxidation as a possible secondary mechanism of sterigmatocystin toxicity.sterigmatocystin (stg), a major secondary metabolite of aspergillus versicolor and a. nidulans, is the precursor of aflatoxin b1. in this study, male albino rats were treated with stg-contaminated diet for 30 days, resulting in reduced levels of glutathione, ascorbic acid, and alpha-tocopherol. the activity of catalase in liver was reduced, whereas an increase in the activities of superoxide dismutase and glutathione peroxidase was observed. the levels of cytochrome p450, cytochrome b5, cytochro ...200111727790
antifungal activities of posaconazole, ravuconazole, and voriconazole compared to those of itraconazole and amphotericin b against 239 clinical isolates of aspergillus spp. and other filamentous fungi: report from sentry antimicrobial surveillance program, 2000.posaconazole, ravuconazole, and voriconazole are new triazole derivatives that possess potent, broad-spectrum antifungal activity. we evaluated the in vitro activity of these investigational triazoles compared with that of itraconazole and amphotericin b against 239 clinical isolates of filamentous fungi from the sentry program, including aspergillus spp. (198 isolates), fusarium spp. (7 isolates), penicillium spp. (19 isolates), rhizopus spp. (4 isolates), mucor spp. (2 isolates), and miscellan ...200211897586
common environmental allergens causing respiratory allergy in india.respiratory allergy affects all age groups but the children are the worst affected by the respiratory allergy. bioparticles from different biological sources are the main cause of allergy. pollen grains, fungal spores, insect and other materials of biological origin form the most important allergen load in the air. for the efficient diagnosis of the allergy and its effective treatment it is very important to know about the prevalence, seasonal and annual variations of aeroallergens of the area. ...200212003301
indoor fungi and their ciliostatic metabolites.according to epidemiological studies, it is possible that some secondary metabolites of indoor airborne fungi could be responsible for health troubles which occupants suffer from. in our previous experiments, a model with tracheal rings of 1-day-old chicks in vitro was shown to be a very suitable method to study the ciliostatic chloroform-extractable endo- and/or exometabolites of filamentous fungi. in this study we isolated the filamentous fungi from walls of "mouldy" dwellings and schools (cul ...200212088399
fungal fragments as indoor air biocontaminants.the aerosolization process of fungal propagules of three species (aspergillus versicolor, penicillium melinii, and cladosporium cladosporioides) was studied by using a newly designed and constructed aerosolization chamber. we discovered that fungal fragments are aerosolized simultaneously with spores from contaminated agar and ceiling tile surfaces. concentration measurements with an optical particle counter showed that the fragments are released in higher numbers (up to 320 times) than the spor ...200212089037
occurrence of toxigenic aspergillus versicolor isolates and sterigmatocystin in carpet dust from damp indoor environments.over the past decade, there has been growing concern regarding the role of toxigenic fungi in damp indoor environments; however, there is still a lack of field investigations on exposure to mycotoxins. the goal of our pilot study was to quantify the proportion of toxigenic aspergillus versicolor isolates in native carpet dust from damp dwellings with mold problems and to determine whether sterigmatocystin can be detected in this matrix. carpet dust samples (n = 11) contained from <2.5 x 10(1) to ...200212147486
apparent antifungal activity of several lactic acid bacteria against penicillium discolor is due to acetic acid in the medium.fifty-six dairy bacteria belonging to the genera lactococcus, lactobacillus, pediococcus, propionibacterium, streptococcus, enterococcus, leuconostoc, and brevibacterium were screened for antifungal activity against four species of fungi relevant to the cheese industry (penicillium discolor, penicillium commune, penicillium roqueforti, and aspergillus vesicolor). most of the active strains belonged to the genus lactobacillus, whereas penicillium discolor was found to be the most sensitive of the ...200212182485
synthesis and antifungal and antioxidant properties of some new 5-substituted-4-amino(or aryl)-3-mercapto-4(h)-1,2,4-triazoles.the synthesis and antifungal and antimicrobial evaluation of some new 5-substituted-4-amino(or aryl)-3-mercapto-4(h)-1,2,4-triazoles is reported. the involvement of the new compounds in free radical processes is also investigated and their lipophilicity is calculated and related to the determined antioxidant activity. compounds 7a, 7b and 7c exhibited interesting antioxidant properties at concentration 0.01 mmol/l, expressed by their capacity to inhibit fe2+/ascorbate induced hepatic microsomal ...200212189782
indoor air microbes and respiratory symptoms of children in moisture damaged and reference schools.microbial indoor air quality and respiratory symptoms of children were studied in 24 schools with visible moisture and mold problems, and in eight non-damaged schools. school buildings of concrete/brick and wooden construction were included. the indoor environment investigations included technical building inspections for visible moisture signs and microbial sampling using six-stage impactor for viable airborne microbes. children's health information was collected by questionnaires. the effect o ...200212244747
volatile metabolites from microorganisms grown on humid building materials and synthetic media.growth of different microorganisms is often related to dampness in buildings. both fungi and bacteria produce complicated mixtures of volatile organic compounds that include hydrocarbons, alcohols, ketones, sulfur- and nitrogen-containing compounds etc. microbially produced substances are one possible explanation of odour problems and negative health effects in buildings affected by microbial growth. a mixture of five fungi, aspergillus versicolor, fusarium culmorum, penicillium chrysogenum, ulo ...200212400912
spores of aspergillus versicolor isolated from indoor air of a moisture-damaged building provoke acute inflammation in mouse lungs.microbial growth in moisture-damaged buildings has been associated with respiratory health effects, and the spores of the mycotoxin producing fungus aspergillus versicolor are frequently present in the indoor air. to characterize the potential of these spores to cause harmful respiratory effects, mice were exposed via intratracheal instillation to a single dose of the spores of a. versicolor (1 x 10(5), 1 x 10(6), 5 x 10(6), 1 x 10(7), or 1 x 10(8) spores), isolated from the indoor air of a mois ...200212454790
effect of liner and core materials of plasterboard on microbial growth, spore-induced inflammatory responses, and cytotoxicity in macrophages.microorganisms, when grown on wetted plasterboards, can produce bioactive compounds capable of inducing inflammatory and toxic reactions in mammalian cells. the paper liner of plasterboard is commonly regarded as the major substrate for microbial growth. in this study, we cultured stachybotrys chartarum, aspergillus versicolor, penicillium spinulosum, and streptomyces californicus on liners and cores of plasterboards in order to examine the role of these main plasterboard components on microbial ...200212454792
production of proinflammatory mediators by indoor air bacteria and fungal spores in mouse and human cell lines.we compared the inflammatory and cytotoxic responses caused by household mold and bacteria in human and mouse cell lines. we studied the fungi aspergillus versicolor, penicillium spinulosum, and stachybotrys chartarum and the bacteria bacillus cereus, pseudomonas fluorescens, and streptomyces californicus for their cytotoxicity and ability to stimulate the production of inflammatory mediators in mouse raw264.7 and human 28sc macrophage cell lines and in the human a549 lung epithelial cell line i ...200312515684
novel chromone derivatives from the fungus aspergillus versicolor isolated from the marine sponge xestospongia exigua.from the marine sponge xestospongia exigua collected in indonesia the fungus aspergillus versicolor was isolated. following cultivation in a seawater-based medium seven new angular tricyclic chromone derivatives (1-7) were obtained from the mycelia and culture filtrate. compounds 2-7 contain an additional dihydropyran ring system which is replaced by a pyridine ring in 1. the structures of the new natural products were established on the basis of extensive one- and two-dimensional nmr spectrosco ...200312542346
survival of filamentous fungi in hypersaline dead sea water.a variety of filamentous fungi have recently been isolated from the dead sea (340 g/l total dissolved salts). to assess the extent to which such fungi can survive for prolonged periods in dead sea water, we examined the survival of both spores and mycelia in undiluted dead sea water and in dead sea water diluted to different degrees with distilled water. mycelia of aspergillus versicolor and chaetomium globosum strains isolated from the dead sea remained viable for up to 8 weeks in undiluted dea ...200312545316
effect of building frame and moisture damage on microbiological indoor air quality in school buildings.the effect of building frame and moisture damage on microbial indoor air quality was characterized in 17 wooden and 15 concrete or brick school buildings. technical investigations to detect visible moisture and mold damage were performed according to a standardized protocol. viable airborne microbes were determined by using a six-stage impactor (andersen 10-800). mean concentrations of viable airborne fungi were significantly higher in wooden schools than in concrete schools, showing that the fr ...200312570403
the relation between growth of four microbes on six different plasterboards and biological activity of spores.microbial growth on water-damaged building materials is commonly associated with adverse health effects in the occupants. we examined the growth of stachybotrys chartarum, aspergillus versicolor, penicillium spinulosum, and streptomyces californicus, isolated from water-damaged buildings, on six different brands of plasterboards. the microbial growth was compared with the biological activity of the spores, that is the potential to induce cytotoxicity and proinflammatory mediators in raw264.7 mac ...200312608927
evaluation of a polymerase chain reaction reverse hybridization line probe assay for the detection and identification of medically important fungi in bronchoalveolar lavage fluids.an assay system in which polymerase chain reaction (pcr) amplification of the its-1 region of ribosomal dna (rdna) is combined with a reverse-hybridization line probe assay (lipa) was used for the identification of six candida species and four aspergillus species in pure cultures of clinical isolates, as well as in bronchoalveolar lavage (bal) fluid samples from 42 patients with various underlying diseases. the results were compared with the results obtained with conventional routine identificat ...200312627806
quantifying mold biomass on gypsum board: comparison of ergosterol and beta-n-acetylhexosaminidase as mold biomass parameters.two mold species, stachybotrys chartarum and aspergillus versicolor, were inoculated onto agar overlaid with cellophane, allowing determination of a direct measurement of biomass density by weighing. biomass density, ergosterol content, and beta-n-acetylhexosaminidase (3.2.1.52) activity were monitored from inoculation to stationary phase. regression analysis showed a good linear correlation to biomass density for both ergosterol content and beta-n-acetylhexosaminidase activity. the same two mol ...200312839773
a case of hypersensitivity pneumonitis caused by humicola fuscoatra.a 51-year-old housewife with hypersensitivity pneumonitis caused by humicola fuscoatra is reported. the diagnosis was made by an inhalation challenge with h. fuscoatra antigen. she was admitted for diagnosis and treatment of a fever and productive cough. auscultation of her lungs revealed inspiratory fine crackles. her chest ct showed diffuse miliary nodules in a centri-lobular distribution with patchy ground glass opacities. findings of transbronchial lung biopsy and bal fluid were compatible w ...200312856749
curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant.a presumed antimicrobial enzyme system, the curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. in the presence of hydrogen peroxide, curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. the curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (pseudomonas spp., escherichia coli, serratia marcescens, aeromonas salmonicida, s ...200312902249
[experimental lung carcinoma induced by fungi and mycotoxins--a review].up to now, most of the etiological studies of lung cancer have been focused on air pollution, radiation and smoking, etc, whereas in the past decades, we had found that fungi and mycotoxins in foodstuff might play important roles in the carcinogenesis of lung cancer. our preliminary work showed that the liquid in pickled vegetables consumed by residents of taihang mountain area in hebei province and corn flours inoculated with aspergillus flavus, aspergillus versicolor in which aflatoxin g1(afg1 ...200312920799
volatile metabolites from indoor molds grown on media containing wood constituents.since volatile mold metabolites are used for the detection of mold growth in buildings, it was interesting to determine whether different indoor mold species show different affinity for the major components of wood, a common building material. growth and volatile metabolites were studied when aspergillus versicolor, penicillium chrysogenum, and p. palitans were grown on laboratory substrates containing the major wood constituents cellulose, xylan and lignin. microbial volatile organic compounds ...200312943002
acute effects of aspergillus versicolor aerosols on murine airways.mice were exposed to aerosols from aspergillus versicolor extract by inhalation (for 15-20 min). changes in respiratory function parameters were monitored during the exposure to evaluate acute effects on the upper and lower respiratory tracts and the conductive airways. the single inhalation exposure to a. versicolor aerosols at the range of 12-151 mg/m3 provoked upper respiratory tract irritation in the airways of mice. the higher the exposure concentration the higher was the increase in the se ...200312950589
studies in the biochemistry of micro-organisms. 99. metabolic products of aspergillus versicolor (vuillemin) tiraboschi. 195713403887
studies in mycological chemistry. xvii. averythrin, an anthraquinonoid pigment from aspergillus versicolor (vuillemin) tiraboschi. 196514324400
mould specific igg antibodies connected with sinusitis in teachers of mould damaged school: a two-year follow-up study.the aim of this study was to describe the relationship between mould exposure induced by moisture damage and mould specific immunoglobulin g antibodies to 20 common mould species and their association with respiratory diseases.200314587535
mold-specific immunoglobulin g antibodies in a child population.the determination of immunoglobulin g (igg) antibodies to molds has been used as an objective evidence of significant mold exposure. until present, no data have been published on antibody responses to molds in healthy children living in normal housing conditions. the microbe-specific igg antibody concentrations of 21 molds and 3 actinobacteria were determined by enzyme-linked immunosorbent assay (elisa) in 103 1- to 6-year-old children (12.4% of the population of that age), and in 111 7- to 14-y ...200314641607
evolution of genomic diversity and sex at extreme environments: fungal life under hypersaline dead sea stress.we have found that genomic diversity is generally positively correlated with abiotic and biotic stress levels (1-3). however, beyond a high-threshold level of stress, the diversity declines to a few adapted genotypes. the dead sea is the harshest planetary hypersaline environment (340 g.liter-1 total dissolved salts, approximately 10 times sea water). hence, the dead sea is an excellent natural laboratory for testing the "rise and fall" pattern of genetic diversity with stress proposed in this a ...200314645702
[clinical, mycological and pathogenic observations on three cases of otomycosis with aspergillus versicolor]. 195014772347
synergistic interaction in simultaneous exposure to streptomyces californicus and stachybotrys chartarum.the microbial exposure associated with health complaints in moldy houses consists of a heterogeneous group of components, including both living and dead bacteria, fungi, and their metabolites and active compounds. however, little is known about the interactions between different microbes and their metabolites, although the cytotoxicity and inflammatory potential of certain individual microbes have been reported. in this study, we investigated the inflammatory responses of mouse raw264.7 macropha ...200415121507
fungal spore source strength tester: laboratory evaluation of a new concept.the airborne fungal spore concentration measured with air samplers during specific time intervals does not always adequately represent the maximum spore concentration levels, because of the sporadic nature of spore release. hence, a reliable method is needed to directly assess the indoor fungal sources with respect to their spore aerosolization potential. in this study, the newly developed fungal spore source strength tester (fssst), which aerosolizes spores from growth surfaces and samples the ...200415262159
[the mechanisms of gastric cancer development produced by the combination of helicobacter pylori with sterigmatocystin, a mycotoxin].it is considered that about sixty-five percent of people are suffering from helicobacter pylori infection in our country. in the east asian countries including japan, such fungus as aspergillus are ubiquitously found in the environment as a contaminant in human food stuffs and animal feeds. sterigmatocystin is a mycotoxin and a precursor of aflatoxin which is produced by aspergillus versicolor. the mechanisms of gastric carcinoma development induced by the combination of helicobacter pylori infe ...200415283159
rapid differentiation of aspergillus species from other medically important opportunistic molds and yeasts by pcr-enzyme immunoassay.we developed a pcr-based assay to differentiate medically important species of aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and dna probes in an enzyme immunoassay format (pcr-eia). oligonucleotide probes, directed to the internal transcribed spacer 2 region of ribosomal dna from aspergillus flavus, aspergillus fumigatus, aspergillus nidulans, aspergillus niger, aspergillus terreus, aspergillus ustus, and aspergillus v ...200415297489
amoebae and other protozoa in material samples from moisture-damaged buildings.mold growth in buildings has been shown to be associated with adverse health effects. the fungal and bacterial growth on moistened building materials has been studied, but little attention has been paid to the other organisms spawning in the damaged materials. we examined moist building materials for protozoa, concentrating on amoebae. material samples (n = 124) from moisture-damaged buildings were analyzed for amoebae, fungi, and bacteria. amoebae were detected in 22% of the samples, and they w ...200415364591
mites and fungi in heavily infested stores in the czech republic.toxigenic and allergen-producing fungi represent a serious hazard to human food and animal feed safety. ninety-four fungal species were isolated from mite-infested samples of seeds taken from czech seed stores. fungi were isolated from the surface of four kinds of seeds (wheat, poppy, lettuce, and mustard) and from the gut and external surface of five species of mites (i.e., acarus siro l., 1758, caloglyphus rhizoglyphoides (zachvatkin, 1973), lepidoglyphus destructor (schrank, 1781), tyrophagus ...200415666776
a simple multiplex polymerase chain reaction assay for the identification of four environmentally relevant fungal contaminants.historically, identification of filamentous fungal (mold) species has been based on morphological characteristics, both macroscopic and microscopic. these methods may often be time-consuming and inaccurate, necessitating the development of identification protocols that are rapid, sensitive, and precise. the polymerase chain reaction (pcr) has shown great promise in its ability to identify and quantify individual organisms from a mixed culture environment; however, the cost effectiveness of singl ...200515676191
pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices.microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ...200415691161
assessment of ribosomal large-subunit d1-d2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important aspergillus species.molecular approaches are now being developed to provide a more rapid and objective identification of fungi compared to traditional phenotypic methods. ribosomal targets, especially the large-subunit rna gene (d1-d2 region) and internal transcribed spacers 1 and 2 (its1 and its2 regions), have shown particular promise for the molecular identification of some fungi. we therefore conducted an assessment of these regions for the identification of 13 medically important aspergillus species: aspergill ...200515872227
biotic and abiotic degradation behaviour of ethylene glycol monomethyl ether (egme).glycol ethers are widely used in many processes in the chemical industry. their high water solubility means they are used as solvents for different purposes (e.g. lacquers and varnishes). since glycol ethers are known to produce toxic metabolites such as the teratogenic methoxyacetic acid during biodegradation, the biological treatment of glycol ethers can be hazardous. however, using oxidizing agents like hydrogen peroxide could be a feasible option for treating wastewater containing glycol eth ...200515878604
the production and characterization of monoclonal antibodies to the fungus aspergillus versicolor.fungal exposure measurements in indoor environments require accurate and precise monitoring methods. such techniques may be based on monoclonal antibodies (mabs) and enzyme-linked immunosorbent assays (elisa) and here we report the cross-reactivity patterns of mabs produced against aspergillus versicolor. balb/c mice were immunized with the particulate fraction of homogenized spores and 46 mabs (35 igm, nine igg3, two igg1) were produced and tested for cross-reactivity against 55 fungal species. ...200515910525
bacterial strains from moldy buildings are highly potent inducers of inflammatory and cytotoxic effects.we aimed to identify inflammatory and cytotoxic potential of individual indoor air bacterial and fungal strains, as well as extracts of indoor air filter samples containing bacteria and fungi. mouse raw264.7 macrophages were exposed in vitro to four bacterial strains; streptomyces californicus, mycobacterium terrae, bacillus cereus and pseudomonas fluorescens, and three fungal strains; penicillium spinulosum, aspergillus versicolor and stachybotrys chartarum. furthermore, raw264.7 macrophages we ...200515910531
determination of microbial volatile organic compounds adsorbed on house dust particles and gypsum board using spme/gc-ms.adsorption of microbial volatile organic compounds (mvocs) on house dust was analyzed by performing solid phase microextraction (spme) in combination with gas chromatography-mass spectrometry (gc-ms). settled dust samples were exposed to five selected mvocs and to cultures of the molds stachybotrus chartarum, aspergillus versicolor, and chaetomium globosum. considerable desorption of the mvocs adsorbed on the dust occurred spontaneously within a few hours at room temperature and within several d ...200515954244
rapid counting of liquid-borne microorganisms by light scattering spectrometry.fast and sensitive techniques are needed to determine microorganism presence in liquid samples. in this research, the feasibility of using light scattering spectrometry for enumerating the biological particles in liquid samples was investigated. a particle size spectrometer was used to count six commonly found microbial species suspended in liquid with and without microbiological stains applied: pseudomonas fluorescens, micrococcus spp. vegetative cells and bacillus subtilis var. niger endospore ...200516028880
improvement of xylanase production in solid-state fermentation by alkali tolerant aspergillus versicolor mku3.to optimize the media components for xylanase production by aspergillus versicolor mku3 in solid-state fermentation (ssf).200516033517
screening of flocculant-producing strains by ntg mutagenesis.screening of new microorganism being able to produce efficiently flocculants was carried out. a new model for screening efficient flocculant-producing strains was designed and tested. the results showed that this model for screening efficient flocculant-producing strains is very reliable and can greatly shorten the screening period. 13 flocculant-producing strains were isolated from activated sludge by conventional method. a strain, designated as hhe6, produced the bioflocculant with the turbidi ...200516083133
effects of microbial cocultivation on inflammatory and cytotoxic potential of spores.microbial growth on moisture-damaged building materials is commonly associated with adverse health effects in the occupants. in moisture damage situations, the environmental conditions as well as the dominant microbial species will vary, leading to a diversity of microbes and continual changes in the different microbial populations. currently, very little is known about the effects of microbial cocultures on the potential harmfulness of the microbial population. in this study we have investigate ...200516087574
immunoglobulin g antibodies against environmental moulds in a norwegian healthy population shows a bimodal distribution for aspergillus versicolor.immunoglobulin g (igg) antibodies against moulds related to indoor dampness problems are used as biomarkers to indicate exposure. in the present study, we evaluated the frequency of mould exposure in an adult healthy population by examining levels of mould-specific igg antibodies in norwegian blood donors. using enzyme-linked immunosorbent assay, 106 blood donor sera were analyzed for igg antibodies to aspergillus versicolor, penicillium chrysogenum, cladosporium herbarum, stachybotrys chartarum ...200516179015
endogenous aspergillus versicolor endophthalmitis in an immuno-competent hiv-positive patient.a 25-year-old white, hiv-positive, immuno-competent man was referred to us because of a progressive blurred vision in his right eye. clinical characteristics were suggestive for an unilateral fungal endophthalmitis, and thereby fluconazole firstly, followed by conventional amphotericin b were intravenously administered, without any significant improvement. thus, a pars plana vitrectomy was performed. aspergillus versicolor was isolated from the cultures of the vitreous sample and intravenous lip ...200516205976
nonpathogenic, environmental fungi induce activation and degranulation of human eosinophils.eosinophils and their products are probably important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to certain organisms. an association between environmental fungal exposure and asthma has been long recognized clinically. although products of microorganisms (e.g., lipopolysaccharides) directly activate certain inflammatory cells (e.g., macrophages), the mechanism(s) that triggers eosinophil degranulation is unknown. in this study we investigated whe ...200516210651
mold infestation of wet spray-applied cellulose insulation.mold investigations were conducted in four buildings that had been insulated with wet spray-applied cellulose insulation (wsaci). bulk wsaci samples were collected and analyzed by quantitative polymerase chain reaction (qpcr) methods. airborne mold was evaluated using both burkard total mold spore and andersen culturable/viable sampling methods. although reportedly treated with biocidal borates, qpcr analyses indicated that elevated concentrations of mold cells (reported as spore equivalents per ...200616499151
identification of volatile metabolites from five fungal species cultivated on two media.five fungal species, aspergillus versicolor, penicillium commune, cladosporium cladosporioides, paecilomyces variotii, and phialophora fastigiata, were cultivated on two media, malt extract agar and dichloran glycerol agar. culture flasks provided with inlet and outlet tubes were used and purified, and humidified air was constantly led through the flasks. air samples from the cultures were sorbed on tenax gr and analyzed by thermal desorption-gas chromatography. the produced volatile metabolites ...199516535095
[study on the quantitative and qualitative composition of fungi in dried medicinal plants].the quantitative and qualitative composition of fungi was determined in selected dried medicinal plants purchased in one of the herbal shops in szczecin, poland. the samples examined were as follows: chamomile (flos chamomillae), peppermint (folium menthae piperitae), lemon balm (folium melissae), st. john's wort (herba hyperici), and two herbal mixtures. the fungal composition depended on the specified sample. xerophilic fungi, i.e. eurotium amstelodami, e. herbariorum, e. rubrum and wallemia s ...200516610669
effects of amoebae on the growth of microbes isolated from moisture-damaged buildings.dampness, moisture, and mold in buildings are associated with adverse health outcomes. in addition to fungi and bacteria, amoebae have been found in moisture-damaged building materials. amoebae and a growing list of bacteria have been shown to have mutual effects on each other's growth, but the interactions between amoebae and microbes common in moisture-damaged buildings have not been reported. we co-cultivated the amoeba acanthamoeba polyphaga with bacteria and fungi isolated from moisture-dam ...200616699589
growth of aspergillus versicolor on higher paraffins. 193216744796
[comparative morphological, ecological, and molecular studies of aspergillus versicolor (vuill.) tiraboschi strains isolated from different ecotopes].cultural, morphological, ecological, and trophic properties (growth at different temperatures and on various organic substrates), as well as molecular and genetic peculiarities of aspergillus versicolor (vuill) tiraboschi strains of different origins, were determined. the strains were isolated from different ecotopes (upper horizons of modern soils of several geographic regions, ancient soils and peat, and permafrost). no essential distinctions in cultural and morphological properties were revea ...200616758871
chemical characterization and quantification of siderophores produced by marine and terrestrial aspergilli.ten aspergilli (five each from marine and terrestrial habitats) were screened for siderophore production. all test isolates produced siderophores as indicated by a positive reaction in the fecl(3) test, chrome azurol sulphonate assay, and chrome azurol sulphonate agar plate test. further, the test isolates were compared for their siderophore production potential and chemical characteristics. examination of the chemical nature of the siderophores revealed that all test isolates produced hydroxama ...200616788730
presence of opportunistic oil-degrading microorganisms operating at the initial steps of oil extraction and handling.hydrocarbon-degrading microorganisms from natural environments have been isolated and identified using culture-dependent or molecular techniques. however, there has been little research into the occurrence of microorganisms incorporated into crude oil in the initial steps of extraction and handling, which can reduce the quality of stored petroleum. in the present study, a packed-column reactor filled with autoclaved perlite soaked with crude oil was subjected to a continuous flow of sterile medi ...200616835842
[moulds and yeasts in bottled water and soft drinks].some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ...200617037258
a two-stage cyclone using microcentrifuge tubes for personal bioaerosol sampling.personal aerosol samplers are widely used to monitor human exposure to airborne materials. for bioaerosols, interest is growing in analyzing samples using molecular and immunological techniques. this paper presents a personal sampler that uses a two-stage cyclone to collect bioaerosols into disposable 1.5 ml eppendorf-type microcentrifuge tubes. samples can be processed in the tubes for polymerase chain reaction (pcr) or immunoassays, and the use of multiple stages fractionates aerosol particles ...200617075620
the effect of egyptian honeybee propolis on the growth of aspergillus versicolor and sterigmatocystin biosynthesis in ras cheese.propolis is a natural product collected by honeybee workers. the product was tested for its antifungal effect against aspergillus versicolor atcc 12996 as well as biosynthesis of sterigmatocystin during ripening of egyptian ras cheese. the use of different concentrations of aqueous propolis extract 250, 500 and 1000 part per million (ppm) on the cheese surface was investigated. mould growth and toxin production were completely inhibited at the highest concentration 1000 ppm, while the lower conc ...200717210093
secretion of keratinolytic enzymes and keratinolysis by scopulariopsis brevicaulis and trichophyton mentagrophytes: regression analysis.a survey on keratinophilic fungi from poultry-farm soils at namakkal and from feather dumping soils at chennai, india, revealed the existence of 34 species of fungi. most of the fungi exhibited variable efficiency in producing extracellular keratinase when grown in plates with chicken feathers as the sole carbon and nitrogen source. the fungi aspergillus flavus, aspergillus niger, aspergillus versicolor, chrysosporium state of arthroderma tuberculatum, paecilomyces carneus, scopulariopsis brevic ...200617215897
protracted aspergillus versicolor endophthalmitis caused by corneal microperforation.here we describe the characterization of a protracted aspergillus versicolor endophthalmitis caused by a corneal microperforation.200717458801
mass spectrometry-based strategy for direct detection and quantification of some mycotoxins produced by stachybotrys and aspergillus spp. in indoor environments.dampness in buildings has been linked to adverse health effects, but the specific causative agents are unknown. mycotoxins are secondary metabolites produced by molds and toxic to higher vertebrates. in this study, mass spectrometry was used to demonstrate the presence of mycotoxins predominantly produced by aspergillus spp. and stachybotrys spp. in buildings with either ongoing dampness or a history of water damage. verrucarol and trichodermol, hydrolysis products of macrocyclic trichothecenes ...200717483261
kipukasins, nucleoside derivatives from aspergillus versicolor.seven new aroyl uridine derivatives (kipukasins a-g; 1-7) were isolated from solid-substrate fermentation cultures of two different hawaiian isolates of aspergillus versicolor. the structures of compounds 1-7 were determined by analysis of nmr and ms data. the nucleoside portion of lead compound 1 was assigned as uracil-1-beta-d-ribofuranoside by spectral comparison with an authentic standard. the bioactivity of the original a. versicolor extracts was accounted for mainly by the presence of the ...200717608440
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
a small change in the design of a slit bioaerosol impactor significantly improves its collection characteristics.while several methods are available for bioaerosol monitoring, impaction remains the most common one, particularly for collecting fungal spores. earlier studies have shown that the collection efficiency of many conventional single-stage bioaerosol impactors falls below 50% for spores with an aerodynamic diameter between 1.7 and 2.5 microm because their cut-off size is 2.5 microm or greater. the cut-off size reduction is primarily done by substantially increasing the sampling flow rate or decreas ...200717671667
the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species.in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ...200717761478
german environmental survey for children (geres iv)--first results.german environmental surveys (geress) are large scale population studies which have been carried out on adults in 1985/86, 1990/92 and 1998 and on children aged 6-14 years in 1990/92. geres iv is the first survey focussing exclusively on children [becker, k., schulz, c., babisch, w., dürkop, j., roskamp, e., seiwert, m., szewzyk, r., ullrich, d., seifert, b., 2005. german environmental survey for children (geres iv) 2003-2006. pullut. atmos. 188, 475-479]. geres iv included a representative samp ...200717870665
mycotoxins in rice.mycotoxin contamination in rice is usually lower as in wheat or corn. however, there are some reports that rice has been contaminated with mycotoxins such as aflatoxin b1, b2, g1, g2 (afs), citrinin, deoxynivalenol (don), fumonisin b1, b2, b3 (fms), fusarenon-x (fus.-x), nivalenol (niv), ochratoxin a (ota), sterigmatocystin (ste), and zearalenone. rice in japan is preserved in warehouses where moisture content and temperature are regulated. therefore, mycotoxin contamination from post harvest fu ...200717913273
aerosolization of particulate (1-->3)-beta-d-glucan from moldy materials.mold-damaged building materials may contain biologically active agents, such as (1-->3)-beta-d-glucan, allergens, and mycotoxins, which have been associated with adverse health effects. the release of these components from contaminated surfaces into the air is not well understood. the purpose of this study was to characterize the release of particulate (1-->3)-beta-d-glucan from the surface of artificially mold-contaminated materials. aspergillus versicolor and stachybotrys chartarum were grown ...200818065630
a study on the adsorption mechanism of mercury on aspergillus versicolor biomass.the adsorption behavior of mercury on aspergillus versicolor biomass (avb) has been investigated in aqueous solution to understand the physicochemical process involved and to explore the potentiality of avb in pollution control management. this biomass has been successfully used for reducing the mercury concentration level in the effluent of chloralkali and battery industries to a permissible limit. the results establish that 75.6 mg of mercury is adsorbed per gram of biomass. the adsorption pro ...200718200852
identification of spore allergens from the indoor mould aspergillus versicolor.indoor mould growth and dampness are associated with respiratory health effects and allergies and several studies demonstrated that mainly aspergillus versicolor and penicillium expansum are responsible for indoor mould exposure. in contrast, commercialized test systems to diagnose allergic reactions to this mould species are not available. in this study, allergenic proteins from spores of the indoor relevant species a. versicolor and p. expansum should get detected and identified.200818315733
screening of aspergillus species for occurrence of lectins and their characterization.ten species of aspergillus were screened for occurrence of lectins. each of the species was investigated for the occurrence of extracellular, surface-bound and intracellular lectin activities. as many as four species namely, aspergillus niger, aspergillus versicolor, aspergillus rugulosus and aspergillus nidulans, were found to possess intracellular lectin activities, while none of the species showed extracellular or surface-bound lectin activities. each of the lectin was characterized with resp ...200818383222
isolation, structure elucidation, and biomimetic total synthesis of versicolamide b, and the isolation of antipodal (-)-stephacidin a and (+)-notoamide b from aspergillus versicolor nrrl 35600. 200818389509
[indoor moulds: results of the environmental study in office rooms].the aim of the study was to quantitatively and qualitatively identify moulds occurring in the air of selected office rooms.200718421934
quantitative pcr analysis of fungi and bacteria in building materials and comparison to culture-based analysis.prolonged moisture on building materials can lead to microbial growth on them. microbes can emit spores, metabolites and structural parts into the indoor air and thus, cause adverse health effects of people living and working in these buildings. so far, culture methods have been used for assessment of microbial contamination of building materials. in this work, we used quantitative pcr (qpcr) for the detection of selected fungal and bacterial groups in 184 building materials of different types a ...200818449403
[unusual fungal species causing onychomycosis.].dermatomycoses caused by non-dermatophytic mycelial fungi are very rare with the exception of onychomycoses. controversies regarding the pathogenic role often arise when these species and yeasts are isolated from nail scrapings. candida albicans and candida parapsilosis are the species identified more frequently from nails, particularly from finger nails. because they could be resident flora of the skin, cultures should be interpreted according to clinical data, direct microscopic observation of ...199918473597
mycobiota and aflatoxins in raw materials and pet food in brazil.commercial feedstuffs are a basic element in modern pet husbandry in the world. in dogs, the effect of mycotoxins is severe and can lead to death. few reports on the influence of dietary mycotoxins were found in the scientific literature. the aims of this work were to isolate and identify the mycoflora and to determine the aflatoxins (afs) natural occurrence in raw materials and ready dry pet food. therefore, the aflatoxigenic capacity of aspergillus flavus species was investigated. aspergillus ...200818477320
antimicrobial activity of paenibacillus polymyxa sce2 against some mycotoxin-producing fungi.the purpose of this study was to investigate the antagonistic activity of paenibacillus polymyxa strain sce2 against mycotoxigenic fungi and to characterize the antimicrobial compound.200818498348
effect of gaseous chlorine dioxide on indoor microbial contaminants.traditional and modern techniques for bioaerosol enumeration were used to evaluate the relative efficiency of gaseous chlorine dioxide (clo2) in reducing the indoor microbial contamination under field and laboratory conditions. the field study was performed in a highly microbially contaminated house, which had had an undetected roof leak for an extended period of time and exhibited large areas of visible microbial growth. air concentrations of culturable fungi and bacteria, total fungi determine ...200818512442
development of a novel pcr assay for the identification of the black yeast, exophiala (wangiella) dermatitidis from adult patients with cystic fibrosis (cf).cystic fibrosis (cf) patients may suffer increased morbidity and mortality through colonisation, allergy and invasive infection from fungi. the black yeast, exophiala dermatitidis (synonym wangiella dermatitidis) has been found with increasing frequency in sputum specimens of cf patients, with reported isolation rates ranging from 1.1 to 15.7%. at present, no diagnostic pcr exists to aid with the clinical laboratory detection and identification of this organism. a novel species-specific pcr-base ...200818571996
determination of volatile metabolites originating from mould growth on wall paper and synthetic media.microbial volatile organic compounds (mvocs) emitted from the mould species penicillium expansum, p. chrysogenum, aspergillus versicolor, a. fumigatus, a. niger and cladosporium cladosporoides were analyzed by means of solid phase microextraction (spme) and gcms. the mould species were cultivated on the synthetic agar dichloran chloramphenicol (dg 18) and on wet wall paper. the production of mvocs was monitored over several weeks to detect changes in the emission rates between the initial stage ...200818577403
interaction of chromium with resistant strain aspergillus versicolor: investigation with atomic force microscopy and other physical studies.the interaction of chromium and a chromate resistant aspergillus versicolor strain has been studied by atomic force (afm) and transmission electron (tem) microscopies. the nanomechanical properties such as cell wall rigidity and elasticity were measured by force spectroscopy and found to be 0.61 +/- 0.08 n/m, and 20.5 +/- 2.1 mpa, respectively. on chromium binding, ultrastuctural changes of the cell wall along with the formation of layered structures on the cell wall were observed. tem and afm m ...200818598062
chamber evaluation of a personal, bioaerosol cyclone sampler.a personal cyclone sampler (cyclone) was operated in a 0.9-m3 chamber, side by side with a 25-mm filter sampler (filter) and either a slit impactor (air-o-cell) or a single-stage, multiple-hole, agar impactor (n6). aerosols of two fungal spores were collected for 5 min to 5 hr-aspergillus versicolor: 10, 20, 40, 80, 160, and 320 min; concentration: 10(2)-10(5) spore m(-3); scopulariopsis brevicaulis: 5, 10, 15, 20, 25, and 30-min; concentration: 10(3)-10(5) spore m(-3) (six replicates for each s ...200818720289
preparation of a reference material containing sterigmatocystin.to assure the homogeneity of a reference material for the mycotoxin sterigmatocystin (ste), a study was first conducted to prepare a reference material of rice containing the colouring food red 106. the protocol developed was then used to prepare a reference material of rice containing ste. initially, a v-shaped mixer was used to mix food red 106 and ground brown rice, but the resulting mixture was non-homogeneous. however, when a shakemaster was used for the simultaneous grinding and mixing of ...200818798039
size-fractionated (1-->3)-beta-d-glucan concentrations aerosolized from different moldy building materials.release of submicrometer-sized fungal fragments (<1.0 microm) was discovered in earlier studies, which investigated the aerosolization of spores from moldy surfaces. however, the contribution of fungal fragments to total mold exposure is poorly characterized. the purpose of this study was to investigate the size-fractionated concentrations of particulate (1-->3)-beta-d-glucan and numbers of particles aerosolized from the surface of artificially mold-contaminated materials using a novel sampling ...200919012949
[aspergillus spp. in risk areas of transplant patients in a university hospital].as a consequence of the increase in the number of immunocompromised patients, cases of aspergillosis, due to the opportunist character of this fungus, have increased considerably. aspergillus fumigatus, aspergillus flavus, and aspergillus niger have been found in air and water samples of the majority of investigated hospitals. the aim of the present study was to investigate the presence of aspergilli in transplant patients areas at the hospital universitario of bogotá, colombia. samples of air w ...200819071892
effects on perceived air quality and symptoms of exposure to microbially produced metabolites and compounds emitted from damp building materials.this work investigated perceived air quality and health effects from exposure to low to high levels of volatile organic compounds (vocs) emitted from damp building materials and a mixture of molds growing on the materials. a mixture of wallemia sebi, fusarium culmorum, penicillium chrysogenum, ulocladium botrytis, and aspergillus versicolor was inoculated on pine wood and particle board. in study 1, each of 27 participants took part in two exposure conditions, one with air from molds growing on ...200919077173
mouse model of aspergillus and alternaria induced rhinosinusitis.fungi are known to induce the production of chemical mediators from respiratory epithelial cells and have been increasingly recognized as important pathogens in sinusitis. however, the exact role of fungi in the pathogenesis of rhinosinusitis has not been clearly established. this study was performed to improve our understanding of the role of fungi in the pathogenesis of rhinosinusitis by developing an animal model of fungus induced rhinosinusitis.200919084360
adsorption behavior of mercury on functionalized aspergillus versicolor mycelia: atomic force microscopic study.the adsorption characteristics of mercury on aspergillus versicolor mycelia have been studied under varied environments. the mycelia are functionalized by carbon disulfide (cs(2)) treatment under alkaline conditions to examine the enhance uptake capacity and explore its potentiality in pollution control management. the functionalized a. versicolor mycelia have been characterized by scanning electron microscopy-energy dispersive x-ray analysis (sem-edxa), attenuated total reflection infrared (atr ...200919115871
treatment of fungal bioaerosols by a high-temperature, short-time process in a continuous-flow system.airborne fungi, termed fungal bioaerosols, have received attention due to the association with public health problems and the effects on living organisms in nature. there are growing concerns that fungal bioaerosols are relevant to the occurrence of allergies, opportunistic diseases in hospitals, and outbreaks of plant diseases. the search for ways of preventing and curing the harmful effects of fungal bioaerosols has created a high demand for the study and development of an efficient method of ...200919201954
cottoquinazoline a and cotteslosins a and b, metabolites from an australian marine-derived strain of aspergillus versicolor.an australian marine-derived isolate of aspergillus versicolor (mst-mf495) yielded the known fungal metabolites sterigmatocystin, violaceol i, violaceol ii, diorcinol, (-)-cyclopenol, and viridicatol, along with a new alkaloid, cottoquinazoline a (1), and two new cyclopentapeptides, cotteslosins a (2) and b (3). structures for 1-3 and the known compounds were determined by spectroscopic analysis. the absolute configurations of 1-3 were addressed by chemical degradation and application of the c(3 ...200919245260
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